Sigma 2-16KHL Operating Manual

Translation of the original operating manual
sb

02/2013
Rev. 1.19 of 25/04/2019
Sigma 2-16KHL; ;

part no. 0700802

Sigma 2-16KHL

from serial no. 147260

Refrigerated Centrifuge
with Heating System

Operating Manual

Please retain for later use!

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In case of inquiries, please state the following numbers:

Order number:
Serial number:

© Copyright by
Sigma Laborzentrifugen GmbH
An der Unteren Söse 50
37520 Osterode am Harz
Germany

Tel.: [phone] / 5007-0
Fax: [phone] / 5007-12
Web: www.sigma-zentrifugen.de
E-mail: [e-mail]

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Table of contents

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1 General information ............................................................................................................. 9

1.1 Importance of the operating manual .................................................................................. 9

1.2 Intended use ..................................................................................................................... 9

1.3 Warranty and liability......................................................................................................... 9

1.4 Copyright ........................................................................................................................ 10

1.5 Standards and regulations .............................................................................................. 10

1.6 Scope of supply .............................................................................................................. 10

2 Layout and mode of operation .......................................................................................... 11

2.1 Layout of the centrifuge .................................................................................................. 11

2.1.1 Functional and operating elements ............................................................................ 11

2.1.2 Name plate ................................................................................................................ 12

2.2 Mode of operation ........................................................................................................... 13

2.2.1 Centrifugation principle .............................................................................................. 13

2.2.2 Area of application ..................................................................................................... 13

2.2.2.1 Speed, radius, and relative centrifugal force ...................................................... 14

2.2.2.2 Density ............................................................................................................... 14

3 Safety .................................................................................................................................. 15

3.1 Marking of the unit ................................ .......................................................................... 15

3.2 Explanation of the symbols and notes ............................................................................. 16

3.3 Responsibility of the operator .......................................................................................... 17

3.4 Operating personnel ....................................................................................................... 17

3.5 Informal safety instructions ............................................................................................. 17

3.6 Safety instructions .......................................................................................................... 18

3.6.1 Electrical safety ......................................................................................................... 18

3.6.2 Mechanical safety ...................................................................................................... 18

3.6.3 Fire prevention ........................................................................................................... 19

3.6.4 Chemical and biological safety .................................................................................. 19

3.6.5 Safety instructions for centrifugation .......................................................................... 20

3.6.6 Resistance of plastics ................................................................................................ 20

3.6.7 Safety of rotors and accessories ................................................................................ 21

3.6.7.1 Service life ................................................................ ......................................... 21

3.7 Safety devices ................................................................................................................ 22

3.7.1 Lid lock device ........................................................................................................... 22

3.7.2 Standstill monitoring system ...................................................................................... 22

3.7.3 System check ............................................................................................................ 22

3.7.4 Earth conductor check ............................................................................................... 22

3.7.5 Imbalance monitoring system .................................................................................... 22

3.7.6 Temperature monitoring system ................................................................................ 22

3.7.7 Rotor monitoring system ............................................................................................ 22

3.8 Measures in the event of hazards and accidents ............................................................ 23

3.9 Remaining hazards ......................................................................................................... 23

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4 Storage and transport ....................................................................................................... 24

4.1 Dimensions and weight ................................................................................................... 24

4.2 Storage conditions .......................................................................................................... 24

4.3 Notes on transport ................................ .......................................................................... 24

4.4 Packaging ....................................................................................................................... 25

4.5 Transport safety device ................................................................................................... 25

5 Set-up and connection ...................................................................................................... 26

5.1 Installation site ................................................................................................ ................ 26

5.2 Power supply .................................................................................................................. 26

5.2.1 Type of connection .................................................................................................... 26

5.2.2 Customer-provided fuses ........................................................................................... 27

6 Using the centrifuge .......................................................................................................... 28

6.1 Initial start-up .................................................................................................................. 28

6.2 Switching the centrifuge on ............................................................................................. 28

6.2.1 Opening and closing the lid ........................................................................................ 28

6.2.2 Installation of rotors and accessories ......................................................................... 28

6.2.2.1 Installation of a rotor .......................................................................................... 28

6.2.2.2 Installation of an angle rotor with a hermetic lid .................................................. 29

6.2.2.3 Installation of the microhaematocrit rotor............................................................ 30

6.2.2.4 Installation of accessories .................................................................................. 31

6.2.2.5 Adapters ............................................................................................................ 33

6.2.2.6 Vessels .............................................................................................................. 33

6.3 Control system "Spincontrol L" ........................................................................................ 34

6.3.1 User interface ............................................................................................................ 34

6.3.2 Manual mode ............................................................................................................. 35

6.3.2.1 Starting a centrifugation run ............................................................................... 35

6.3.2.2 Interrupting a centrifugation run ......................................................................... 35

6.3.2.3 Interrupting a deceleration process .................................................................... 35

6.3.2.4 Selection, display, and modification of data ........................................................ 35

6.3.2.1 Speed / relative centrifugal force (RCF) ............................................................. 36

6.3.2.2 Runtime ............................................................................................................. 36

6.3.2.3 Temperature ("Temp") ....................................................................................... 38

6.3.2.4 Rotor selection ................................................................................................... 40

6.3.2.5 Acceleration and deceleration curves ................................................................. 41

6.3.2.6 Automatic lid opening function ("AutoOpen") ...................................................... 41

6.3.2.7 Sound signal ("Buzzer") ..................................................................................... 42

6.3.2.8 Program lock ("ProgLock") ................................................................................. 42

6.3.2.9 Cycle display ("Cycles") ..................................................................................... 43

6.3.2.10 Input lock ........................................................................................................... 44

6.3.3 Program mode ........................................................................................................... 44

6.3.3.1 Saving a program ............................................................................................... 45

6.3.3.2 Loading a program ............................................................................................. 45

6.4 Switching the centrifuge off ............................................................................................. 46

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7 Malfunctions and error correction .................................................................................... 47

7.1 General malfunctions ...................................................................................................... 47

7.1.1 Emergency lid release ............................................................................................... 48

7.2 Table of error codes ........................................................................................................ 49

7.3 Service contact ............................................................................................................... 50

8 Maintenance and service ................................................................................................... 51

8.1 Maintenance ................................................................................................................... 51

8.1.1 Centrifuge .................................................................................................................. 51

8.1.1.1 Condenser (only refrigerated centrifuges with an air-cooled refrigeration

system) .............................................................................................................. 52

8.1.2 Accessories ............................................................................................................... 52

8.1.2.1 Plastic accessories ............................................................................................ 53

8.1.3 Rotors, buckets and carriers ...................................................................................... 53

8.1.4 Microhaematocrit rotor ............................................................................................... 53

8.1.5 Load-bearing bolts ..................................................................................................... 54

8.1.6 Glass breakage ......................................................................................................... 55

8.2 Sterilisation and disinfection of the rotor chamber and accessories ................................ 55

8.2.1 Autoclaving ................................................................................................................ 56

8.3 Service ........................................................................................................................... 57

8.4 Return of defective centrifuges or parts .......................................................................... 58

9 Disposal .............................................................................................................................. 60

9.1 Disposal of the centrifuge ............................................................................................... 60

9.2 Disposal of the packaging ............................................................................................... 60

10 Technical data .................................................................................................................... 61

10.1 Ambient conditions ......................................................................................................... 62

10.2 Technical documentation ................................................................................................ 62

11 Appendix ............................................................................................................................ 63

11.1 Range of accessories ..................................................................................................... 63

11.1.1 Rotor radii .................................................................................................................. 63

11.2 Speed-gravitational-field-diagram ................................ ................................................... 65

11.3 Acceleration and deceleration curves ............................................................................. 66

11.4 Table of the service life of rotors and accessories ........................................................... 67

11.5 Resistance data .............................................................................................................. 68

11.6 EC declaration of conformity ........................................................................................... 73

11.7 Declaration of conformity – China RoHS 2 ...................................................................... 75

12 Index ................................................................................................................................... 77

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1 General information

1.1 Importance of the operating manual

A fundamental requirement for the safe and trouble-free operation of the
centrifuge is to be familiar with the fundamental safety instructions and all
possible hazards.

The operating manual includes important information concerning the safe
operation of the centrifuge.

This operating manual and, in particular, the notes on safety and hazards
must be observed by all persons operating the centrifuge.

In addition, the local rules and regulations for the prevention of accidents



must be complied with.

1.2 Intended use

Centrifuges are power-driven machines that separate liquids from solid
matter, liquid mixtures, or solid mixtures by centrifugal force. They are
solely intended for this purpose. Any other use beyond this area of
application is regarded as improper use. Sigma Laborzentrifugen GmbH
cannot be held liable for any damage resulting from such improper use.

The intended use also includes

• observation of all the notes and instructions included in the operating
manual and



• compliance with the care, cleaning, and maintenance instructions.

1.3 Warranty and liability

The warranty and liability are subject to our "General Conditions" that were
distributed to the operator upon the conclusion of the contract.

Warranty and liability claims are excluded if they are due to:

• improper use.

• non-compliance with the safety instructions and hazard warnings in the

operating manual.

• improper installation, start-up, operation, or maintenance of the
centrifuge.

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• 1 power cord with an IEC C13 connector

• 1 rotor wrench

Part no. 930 100

• 1 tube (30 g) heavy-duty grease for load-
bearing bolts

Part no. 71 401



1.4 Copyright

The copyright concerning the operating manual remains with
Sigma Laborzentrifugen GmbH.

The operating manual is solely intended for the operator and their
personnel. It includes instructions and information that must not be

• duplicated,

• distributed, or

• communicated in any other way.



Non-compliance may be prosecuted under criminal law.

1.5 Standards and regulations

EC declaration of conformity
(see chapter 11.6 - "EC declaration of conformity")

1.6 Scope of supply

The centrifuge comprises:

Documentation

Operating manual incl. EC declaration of conformity
(see chapter 11.6 - "EC declaration of conformity")

Accessories

According to your order, our order confirmation, and your delivery note.

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1 Lid
2 User interface (see

chapter 6.3.1 - "User
interface")

3 Mains switch

4 Name plate

(see chapter 2.1.2 ­"Name plate")

5 Mains power input



2 Layout and mode of operation



2.1 Layout of the centrifuge

2.1.1 Functional and operating elements

Fig. 1: Total view of the centrifuge

Fig. 2: Rear view of the centrifuge

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1 Manufacturer
2 Power consumption
3 Max. speed
4 Max. kinetic energy
5 Serial number
6 Part number
7 Type
8 Nominal voltage
9 CE mark in compliance

with the directive
2006/42/EC

10 Symbol for special

disposal (see chapter
9 - "Disposal")

11 Consult operating

manual
12 Date of manufacture
13 Max. density
14 Refrigerant data

2.1.2 Name plate

Fig. 3: Example of a name plate

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

2.2 Mode of operation



2.2.1 Centrifugation principle

Das

Centrifugation is a process for the separation of heterogeneous mixtures of
substances (suspensions, emulsions, or gas mixtures) into their
components. The mixture of substances, which rotates on a circular path, is
subject to centripetal acceleration that is several times greater than the
gravitational acceleration.

Centrifuges use the mass inertia inside the rotor chamber for separating the
substances. Due to their higher inertia, particles or media with a higher
density travel outwards. In doing so, they displace the components with a
lower density, which in turn travel towards the centre.

The centripetal acceleration of an object inside a centrifuge, as the effect of
centripetal force, depends on the distance between the object and the axis
of rotation as well as on the angular velocity. It increases linearly as a
function of the distance with regard to the axis of rotation and quadratically
as a function of the angular velocity. The bigger the radius in the rotor
chamber is and the higher the speed is, the higher the centripetal
acceleration is. However, the forces acting on the rotor also increase.

2.2.2 Area of application

Depending on the area of application of the centrifuge and also on the
particle size, solids content, and volume throughput of the mixture of
substances that is to be centrifuged, there are different types of centrifuges.

The areas of application go from household use as a salad spinner or
honey separator up to specialised technical applications in the clinical,
biological, or biochemical context:

• For numerous clinical examinations, cellular material must be separated
from the liquid to be analysed. The normal separation process can be
sped up considerably by using laboratory centrifuges.

• In the metal-working industry, centrifuges are used for separating oil
from metal cuttings. Dairies use centrifuges in order to separate cow’s
milk into cream and low-fat milk.

• Particularly big centrifuges are used in the sugar industry for separating
the syrup from the crystalline sugar.

• Ultracentrifuges are predominantly used in biology and biochemistry in
order to isolate particles, e.g. viruses. They are specifically designed for
high speeds up to 500,000 rpm. The rotor moves in a vacuum in order
to avoid air friction.

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)/2,1( Rho



2.2.2.1 Speed, radius, and relative centrifugal force

The acceleration g, which the samples are subject to, can be increased by
increasing the radius in the rotor chamber and by increasing the speed.
These three parameters are interdependent and linked with each other via
the following formula:

Relative centrifugal force RCF = 11.18 x 10-6 x r x n2

r = radius in cm
n = speed in rpm
RCF without any dimension

If two values are entered, the third value is determined by way of the stated
formula. If, afterwards, the speed or the radius is changed, the resulting
relative centrifugal force will be recalculated automatically by the control
unit. If the RCF is changed, the speed will be adapted while the specified
radius is maintained.

The speed-gravitational-field-diagram provides an overview of the
relationship between speed, radius, and RCF (see chapter 11.2 - "Speed-



gravitational-field-diagram").

2.2.2.2 Density

The laboratory centrifuge is suitable for the separation of constituents of
different densities in mixtures with a maximum density of 1.2 g/cm3. All
information concerning the speed of rotors and accessories refers to liquids
with a density corresponding to this specification. If the density is above this
value, the maximum permissible speed of the centrifuge must be reduced
based on the following formula:

n = n

Rho = density in g/cm3

max

x

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Dangerous voltage

I

On (Power)

Hot surface

0

Off (Power)

Caution! Risk of bruising

Name plate (see chapter 2.1.2

- "Name plate")

Protective earth (ground)

CE mark in compliance with
the directive 2006/42/EC

Earth (ground)

Do not dispose as part of
domestic waste

Unplug the mains plug

GS mark (tested safety; only
for Germany)

Arrow indicating the direction of
rotation

NRTL mark (only for the USA
and Canada)

Centrifuge with heating system

RCM mark
(only for Australia)

Rotor loading information
(see chapter 6.2.2.4 ­"Installation of accessories")

China RoHS 2 mark
(only for China)

NOTE

Safety indications on the centrifuge must be kept readable at all times. If
necessary, they must be replaced.

NOTE

Not all of the symbols/labels are used for this centrifuge type.



3 Safety



3.1 Marking of the unit

The following symbols are used for all types of centrifuges manufactured by Sigma:



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DANGER

This symbol stands for a direct hazard to the life and health of persons.

Non-observance of these symbols causes serious health problems up to
life-endangering injuries.

DANGER

This symbol stands for a direct hazard to the life and health of persons
due to electrical voltage.

Non-observance of these symbols causes serious health problems up to
life-endangering injuries.

WARNING

This symbol stands for a potential hazard to the life and health of persons.

Non-observance of these symbols can cause serious health problems up
to life-endangering injuries.

CAUTION

This symbol indicates a potentially hazardous situation

Non-observance of these notes can cause minor injuries or damage to
property.

NOTE

This symbol indicates important information.

3.2 Explanation of the symbols and notes

In this operating manual, the following names and symbols to indicate
hazards are used:

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

3.3 Responsibility of the operator

The operator is responsible for authorising only qualified personnel to work
on the centrifuge (see chapter 3.4 - "Operating personnel").

The areas of responsibility of the personnel concerning the operation,
maintenance, and care of the unit must be clearly defined.

The safety-conscious work of the personnel in compliance with the
operating manual and the relevant EC and national health and safety
regulations as well as with the accident prevention regulations must be
checked at regular intervals (e.g. every month).

Under the international rules for health and safety at work, the operator is
obliged to:

• take measures in order to prevent all danger to life or health during
work.

• ensure that centrifuges are operated properly and entirely as intended
(see chapter 1.2 - "Intended use").

• take protective measures against fire and explosion when working with
hazardous substances.



• take measures for the safe opening of centrifuges.

3.4 Operating personnel

Persons operating the unit must

• be familiar with the fundamental regulations concerning workplace
safety and accident prevention

• have read and understood this operating manual (and in particular the
safety sections and warning notes) and confirmed this with their



signature.

3.5 Informal safety instructions

• This operating manual is a part of the product.

• The operating manual must be kept at the location of use of the

centrifuge. Ensure that it is accessible at all times.

• The operating manual must be handed over to any subsequent owner
or operator of the centrifuge.

• Any changes, additions or updates received must be added to the
operating manual.

• In addition to the operating manual, the general and local rules and
regulations concerning the prevention of accidents and the protection of
the environment must also be supplied.

• Safety and danger indications on the centrifuge must be kept readable
at all times. If necessary, they must be replaced.

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DANGER

• Ensure that the wall socket is properly wired and grounded.

• Check that the mains voltage agrees with the nominal voltage listed on

the name plate.

• Do not place vessels containing liquid on the centrifuge lid or within the
safety distance of 30 cm around the centrifuge. Spilled liquids may get
into the centrifuge and damage electrical or mechanical components.

• Work on the power supply system must only be performed by certified
electricians.

• Inspect the electrical equipment of the unit regularly. Defects such as
loose or burnt cables must be eliminated immediately.

WARNING

• Do not open the lid when the rotor is in motion!

• Do not reach into the rotor chamber when the rotor is in motion!

• Do not use the centrifuge if it was installed incorrectly.

• Do not use the centrifuge without panels.

• Do not use the centrifuge if the rotors and inserts show signs of

corrosion or other defects.

• Only use the centrifuge with rotors and accessories that have been
approved by the manufacturer. In case of doubt, contact the
manufacturer (see chapter 7.3 - "Service contact").

• Do not hold your fingers between the lid and the housing when closing
the lid. Risk of crushing!

• Defective lid relieving devices could cause the centrifuge lid to fall
(contact the service department, if necessary). Risk of crushing!

• Do not hit or move the centrifuge during its operation.

• Do not lean against or rest on the centrifuge during its operation.

• Do not spin any substances that could damage the material of the

rotors and buckets of the centrifuge in any way. Highly corrosive
substances, for example, damage the material and affect the
mechanical strength of the rotors and buckets.

• Stop the centrifuge immediately in the event of a malfunction. Eliminate
the malfunction (see chapter 7 - "Malfunctions and error correction") or
inform the service department of the manufacturer (see chapter 7.3 ­"Service contact").

• Ensure that all repairs are performed only by authorised and
specialised personnel.



3.6 Safety instructions



3.6.1 Electrical safety

To reduce the risk of electrical shock, the centrifuge uses a three-wire
electrical cord and plug to connect the equipment to earth-ground. To



preserve this safety feature:

3.6.2 Mechanical safety

In order to ensure the safe operation of the centrifuge, observe the
following:

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WARNING

• Prior to any start-up, check the centrifuge, rotor, and accessories for
signs of damage that can be discerned from the outside. Special
attention must be paid to all of the rubber parts (e.g. motor cover, lid
seal, and adapters) in terms of visible structural changes. Defective
parts must be replaced immediately.

• Open the centrifuge when it is not in use so that moisture can
evaporate.

DANGER

• Do not spin explosive or inflammable substances.

• Do not use the centrifuge within hazardous locations.

DANGER

• Infectious, toxic, pathogenic, and radioactive substances may only be
used in special, certified containment systems with a bio-seal in order
to prevent the material from being released.

• Take suitable precautions for your own safety if there is a risk of toxic,
radioactive, or pathogenic contamination

• Materials that chemically react with each other with a high level of
energy are prohibited.

WARNING

• Keep informed about local measures to avoid harmful emissions
(depending on the substances to be centrifuged).

• Protective clothing is not required for the operation of the centrifuge.
The materials to be centrifuged may, however, require special safety
measures (e.g. centrifugation of infectious, toxic, radioactive, or
pathogenic substances).



3.6.3 Fire prevention



3.6.4 Chemical and biological safety

If pathogenic, toxic, or radioactive samples are intended to be used in the
centrifuge, it is in the responsibility of the user to ensure that all necessary
safety regulations, guidelines, precautions, and practices are adhered to
accordingly.

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WARNING

• Ensure that the centrifuge was set up properly (see chapter 5 - "Set-up
and connection").

• Maintain a safety distance of at least 30 cm (12 inches) around the
centrifuge.

• Do not store any dangerous goods in the centrifuge area.

• Do not stay in the safety area longer than what is absolutely necessary

for the operation of the centrifuge.

• Only use the centrifuge with rotors and accessories that have been
approved by the manufacturer. We explicitly warn against the use of
equipment of poor quality. Breaking glass or bursting vessels can
cause dangerous imbalances at high speeds

• Ensure that rotor and buckets are correctly fitted (see chapter 6.2.2.1 ­"Installation of a rotor").

• Observe the instructions on the installation of accessories (see chapter

6.2.2.4 - "Installation of accessories").

WARNING

• The rotor must be loaded in a rotationally symmetrical manner at equal
weights.

• If liquids with a density > 1.2 g/cm3 are used, reduce the speed (see
chapter 2.2.2.2 - "Density").

• Do not use the centrifuge if the rotor is loaded asymmetrically.

• Do not use the centrifuge with tubes that are excessively long.

NOTE

• Refer to the resistance data (see chapter 11.5 - "Resistance data")!



3.6.5 Safety instructions for centrifugation

For safe operation, observe the following before starting the centrifuge:



3.6.6 Resistance of plastics

Chemical influences have a strong effect on the polymeric chains of
plastics, and, therefore, on their physical properties. Plastic parts can be
damaged if solvents, acids, or alkaline solutions are used.

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WARNING

Perform regular checks (at least once per month) for safety reasons!

• Pay special attention to changes, such as corrosion, cracks, material
abrasion, etc.

• After 10 years, they must be inspected by the manufacturer.

• After 50,000 cycles, the rotor must be scrapped for reasons of safety.

• If other data concerning the service life are engraved on the rotor or

bucket, these data shall apply accordingly. For example, a bucket with
the engraving "max. cycles = 10,000" has a service life of 10,000
cycles, and a rotor with the engraving "Exp. date 02/20" must be
scrapped in February 2020 at the latest (see figure).

• If a specification concerning the maximum number of cycles and a
specification concerning the service life (i.e. a date) are provided, the
specification that occurs first shall apply.

NOTE

• Refer to the table of rotors and accessories with a different service
life (see chapter 11.4 - " Table of the service life of rotors and
accessories ")!

3.6.7 Safety of rotors and accessories

3.6.7.1 Service life

The rotors and accessories have a limited service life.

Fig. 4: Different service life – engraving on the bucket/rotor

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3.7 Safety devices

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3.7.1 Lid lock device

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3.7.2 Standstill monitoring system

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3.7.3 System check

The centrifuge can only be started when the lid is properly closed. The
electrical lock must be locked. The lid can only be opened when the rotor
has stopped. If the lid is opened by way of the emergency release system
during operation, the centrifuge will immediately switch off and decelerate
brakeless. If the lid is open, the drive is completely separated from the
mains power supply, i.e. the centrifuge cannot be started (see chapter 7.1.1

- "Emergency lid release").

Opening of the centrifuge lid is only possible if the rotor is at a standstill.
This standstill is checked by the microprocessor.

An internal system check monitors the data transfer and sensor signals with
regard to plausibility. The system continuously performs a self-check and
identifies malfunctions. Error messages are displayed as "Error" followed
by a code number (see chapter 7.2 - "Table of error codes").

3.7.4 Earth conductor check

An earth conductor check can be carried out by authorised and specialised
personnel using a suitable measuring instrument. Please contact the Sigma

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service department (see chapter 7.3 - "Service contact").

3.7.5 Imbalance monitoring system

The indication "Imbalance" in the rotor field and, in some cases, also a
sound signal indicate that the centrifuge is in the impermissible imbalance
range.The drive will be switched off in the acceleration phase or during the

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run.

3.7.6 Temperature monitoring system

If the temperature inside the rotor chamber rises above 50°C, the drive
system will be switched off automatically. The centrifuge cannot be

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restarted until it has cooled.

3.7.7 Rotor monitoring system

When a rotor number and, if applicable, a bucket number are selected, the
computer will automatically check whether the entered speed or the
entered gravitational field are permissible for the selected rotor.

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DANGER

• If an emergency arises, switch off the centrifuge immediately!

• If in doubt, call the emergency doctor!

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3.8 Measures in the event of hazards and accidents

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3.9 Remaining hazards

The centrifuge was built in accordance with the state of the art and in
compliance with the generally recognized safety rules. However, danger to
life and limb of the operator, or of third parties, or impairments of the unit or
other material assets cannot be completely excluded when the unit is being
used.

• Use the unit only for the purpose that it was originally intended for (see
chapter 1.2 - "Intended use").

• Use the unit only if it is in a perfect running state.

• Immediately eliminate any problems that can affect safety.

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Sigma 2-16KHL

Height:

310 mm

Height with open lid:

705 mm

Width:

550 mm

Depth:

570 mm

Weight:

60 kg

CAUTION

The centrifuge weighs approx. 60 kg!

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4 Storage and transport

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4.1 Dimensions and weight

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4.2 Storage conditions

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The centrifuge can be stored in its original packaging for up to a year.

• Store the centrifuge only in dry rooms.

• The permissible storage temperature is between -20°C and +60°C.

• If you would like to store it for more than one year, or if you intend to

ship it overseas, please contact the manufacturer.

4.3 Notes on transport

• Install the transport safety device (see chapter 4.5 - "Transport safety
device")

• Always lift the centrifuge with a lifting device or with a sufficient number
of people helping you.

• When lifting the centrifuge, always reach under the centrifuge from the
side.

• For transport use suitable packaging and, if at all possible, the original
packaging (see chapter 4.4 - "Packaging").

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CAUTION

The centrifuge weighs approx. 60 kg!

CAUTION

The transport safety device must be removed prior to start-up!

4.4 Packaging

The centrifuge is packaged in a threepart slip-lid box.

• Take off the lid.

• Remove the box containing the accessories and the packaging material.

• Remove the ring of the slip-lid box.

• Lift the centrifuge upwards with a lifting device or with a sufficient

number of people to lift it safely. When lifting the centrifuge, always

4.5 Transport safety device

reach under the centrifuge from the side.

• Retain the packaging for any possible future transport of the centrifuge.

The transport safety device consists of a foamed plastic piece in the rotor
chamber.

Removal

• Open the lid by pressing the lid key. If the centrifuge is not connected to
the power supply, use the emergency release of the lid (see chapter

7.1.1 - "Emergency lid release").

• Unscrew the rotor tie-down screw.

• Remove the foamed plastic piece from the rotor chamber by lifting it

carefully on one side.

• Retain the transport safety device for the possibility of the return of the
centrifuge.

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DANGER

The operating voltage on the name plate must correspond to the local
supply voltage!

NOTE

The removable power cord must not be replaced with a power cord of
inadequate rating!

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5 Set-up and connection

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5.1 Installation site

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Operate the centrifuge only in closed and dry rooms.
All the energy supplied to the centrifuge is converted into heat and emitted

to the ambient air.

• Ensure sufficient ventilation.

• Keep a safety distance of at least 30 cm around the centrifuge so that

the vents in the centrifuge remain fully effective.

• Do not subject the centrifuge to thermal stress, e.g. by positioning it
near heat generators.

• Avoid direct sunlight (UV radiation).

• The table must be stable and have a solid, even surface.

• Attention: During transport from cold to warmer places, condensational

water will collect inside the centrifuge. It is important to allow sufficient
time for drying (min. 24 h) before the centrifuge can be used again.

5.2 Power supply

5.2.1 Type of connection

Sigma centrifuges are units of protection class I. The centrifuges of this
model series have a three-wire power cord with an IEC C13 connector.

The centrifuges are equipped with a mains power switch with an integrated
thermal circuit breaker.

• Switch the unit off by actuating the mains power switch.

• If it has tripped, let the circuit breaker cool for approximately 2 minutes.

• Switch the unit on.

The centrifuge is now ready for operation.

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5.2.2 Customer-provided fuses

Typically, the centrifuge must be protected with 16 Amp L or B fuses that
are to be provided by the customer.

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DANGER

• Before the initial start-up, please ensure that your centrifuge is properly
set up and installed (see chapter 5 - "Set-up and connection").

WARNING

Do not place your fingers between the lid and the housing when closing
the lid. Risk of crushing!

WARNING

Once a day or after 20 cycles, the rotor tie-down screw must be loosened
by some turns, and the rotor must be lifted and fastened again. This
ensures a proper connection between the rotor and the motor shaft.

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6 Using the centrifuge

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6.1 Initial start-up



6.2 Switching the centrifuge on

• Press the mains power switch.



6.2.1 Opening and closing the lid

The display then illuminates. The centrifuge is ready for operation.

The lid can be opened if the centrifuge is at a standstill and if the lid key is
illuminated.

• Press the lid key in order to open the lid.
The centrifuge cannot be started if the lid is opened.

• To close, press with both hands slightly on the lid until the lock is
audibly locked.



6.2.2 Installation of rotors and accessories



6.2.2.1 Installation of a rotor

• Open the centrifuge lid by pressing the lid key.

• Unscrew the rotor tie-down screw from the motor shaft (counter-

clockwise).

• Lower the rotor with its central bore straight down onto the motor shaft.

• Tighten the rotor tie-down screw clockwise with the supplied rotor

wrench with 3 Nm. In doing so, hold the rotor at its outer rim.

• Follow the safety instructions and hazard warnings (see chapter 3 ­"Safety")!

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CAUTION

When using rotors for microtiter plate formats:
Ensure that the plate holders are inserted together with the plates into the

buckets.

NOTE

The lid screw serves for the fastening of the lid onto the rotor only, not for
the fastening of the rotor onto the motor shaft.

NOTE

The rotor can also be used without a cover.

NOTE

The lid screw serves for the fastening of the lid onto the rotor only, not for
the fastening of the rotor onto the motor shaft.

Removal of a rotor

• Loosen the rotor tie-down screw by turning it anti-clockwise and remove



the rotor.

6.2.2.2 Installation of an angle rotor with a hermetic lid

• Open the centrifuge lid by pressing the lid key.

• Unscrew the rotor tie-down screw from the motor shaft (counter-

clockwise).

• Screw the rotor lid onto the rotor and tighten it by turning it clockwise.

• Lower the rotor with the lid with its central bore straight down onto the

motor shaft.

• Insert the rotor tie-down screw into the motor shaft. Tighten the rotor tie­down screw clockwise with the supplied rotor wrench with 3 Nm. In
doing so, hold the rotor at its outer rim.

• Follow the safety instructions and hazard warnings (see chapter 3 ­"Safety")!

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1 Rotor tie down screw
2 Lid
3 Rotor

CAUTION

Please consider the maximum permissible gravitational field of 12,000 x g.
An excess gravitational field will result in an increased risk of glass
breakage!

Removal of a rotor

• Unscrew the rotor tie-down screw (counter-clockwise) and remove the
rotor with the lid.

6.2.2.3 Installation of the microhaematocrit rotor

• Open the lid by turning it counter-clockwise and remove the lid.

Fig. 5: Angle rotor with a hermetic lid

• Open the centrifuge lid by pressing the lid key.

• Replace the rotor tie-down screw with the rotor tie-down screw of the

microhaematocrit rotor (part no. 81543, included in the scope of supply
of the microhaematocrit rotor), and tighten it loosely.

• Lower the rotor with its central bore straight down onto the motor shaft.

• Tighten the rotor tie-down screw clockwise with 3 Nm using the supplied

rotor wrench. In doing so, hold the microhaematocrit rotor with one hand
and tilt it slightly in order to prevent the motor shaft from slipping
through.

• Check the rotor for a correct and tight fit.

• Follow the safety instructions and hazard warnings (see chapter 3 -

"Safety")!

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Operation

• Fill the capillary tubes with blood and seal them at one end with putty or
by fusion.

• Place the capillary tubes into the recesses of the rotor with the sealed
end against the rubber ring. Ensure that the capillary tubes fit tightly
against the rubber ring. The opposite places must be loaded.

• Screw on the rotor cover.

• Close the centrifuge lid.

• Enter the following parameters: RCF 12,000 x g, runtime approx.

5 minutes.

• Start the centrifuge.

• Open the centrifuge lid when the rotor has stopped.

• Unlock the rotor cover and lift it off.

Evaluation

• Put the reader onto the microhaematocrit rotor.

• Turn the reader and perform a fine adjustment with the aid of the central

eccentric mechanism in order to localize the O-point and the maximum
liquid point in the capillary tubes. You can now read the percentage
value.

• Remove the capillary tubes. Some of the tubes can also be evaluated
with the reader outside of the rotor (see the instructions for use on the



back of the reader).

6.2.2.4 Installation of accessories

• Only use vessels that are suitable for the rotor.

• In the case of swing-out rotors, all of the compartments must be

equipped with buckets.

• Always load opposite compartments of the rotors with the same
accessories and fill to avoid imbalance.

Centrifugation with vessels of various sizes

Working with vessel of various sizes is possible. In this case, however, it is
very important that the rotationally symmetrical inserts are identical.

Fig. 6: Permissible and impermissible loading of a swing-out rotor with vessels of various
sizes (example illustration)

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NOTE

Pay attention to the marking of the centrifuge (see the illustration below)!
Safety indications on the centrifuge must be kept readable at all times. If

necessary, they must be replaced.

Centrifugation with low capacity

• Install the sample vessels in a rotationally symmetrical manner so that
the buckets and their suspensions are loaded evenly.

• It is not permissible to load angle rotors on only one axis.

Fig. 7: Permissible and impermissible loading of an angle rotor and a swing-out rotor
(example illustrations)

Fig. 8: Safety indication on the centrifuge: Loading of a swing-out rotor

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NOTE

When using glass vessels, the maximum value of 4,000 x g must not be
exceeded (except special high-strength glass tubes; please refer to the
information provided by the manufacturer).



6.2.2.5 Adapters

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6.2.2.6 Vessels

In order to ensure easy handling, even if vessels of various sizes are used,
carrier systems were developed.

• Load the opposite adapters with the same number of vessels and with
the same weights in order to avoid imbalance.

• If all of the compartments of a carrier are not used, the buckets must be
loaded evenly. Loading the edges of a bucket only is not permissible.

• Load the vessels outside of the centrifuge. Liquids in the buckets or
multiple carriers cause corrosion.

• Fill the vessels carefully and arrange them according to their weight.
Imbalances result in the excessive wear of the bearings.

• Always fill the tubes up to their useful volume (= the volume that is
stated for the tube).

• Follow the safety instructions and hazard warnings (see chapter 3 ­"Safety")!

Maximum speed for tubes

Some tubes, such as centrifuge glass tubes, microtubes, culture tubes,
fluoropolymer tubes and especially high-volume tubes can be used in our
rotors, buckets, and adapters at higher speeds than their breaking limit.

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1 Start key
2 Function knob
3 Display
4 Stop key
5 Lid key

1 Speed/RCF field
2 Several display fields

(e.g. for rotor,
deceleration curve or

program selection)
3 Time field
4 Temperature field



6.3 Control system "Spincontrol L"



6.3.1 User interface

The centrifuge is operated via three buttons with integrated light-emitting
diodes and one function knob. The display is divided into several different
fields. The various functions of the system can be called up by pressing
and turning the function knob.

Fig. 9: User interface of the Spincontrol L control system

Display

The centrifuge display has the following display fields:

Fig. 10: Display of the Spincontrol L control system

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6.3.2 Manual mode

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6.3.2.1 Starting a centrifugation run



6.3.2.2 Interrupting a centrifugation run



6.3.2.3 Interrupting a deceleration process



6.3.2.4 Selection, display, and modification of data

The centrifuge is ready for operation when the start key is illuminated.

• Press the start key in order to start a centrifugation run.

• Press the stop key in order to interrupt a centrifugation run. The

centrifugation run will be terminated prematurely.

Quick stop

• Press the stop key for more than two seconds.
The centrifuge decelerates with the maximum deceleration curve.
After a quick stop, the centrifuge lid must be opened before a new

centrifugation run can be started.
A quick stop can also be triggered during a normal deceleration, e.g. in

order to speed up the deceleration.
When a quick stop is triggered, ―fast‖ will be displayed in the lower right-

hand corner of the display.

• Press the start key during a deceleration process in order to interrupt it
and to restart the centrifuge.

• Turn the function knob in order to select a field. The selected field will
be preceded by the indication "set", "run", or "save".

• Press the function knob. The indication ("set", "run", or "save") flashes
and the modification mode is active.

• Turn the function knob in order to modify the set value of the selected
field.

• Press the function knob again to confirm the input and to quit the
modification mode

• The process will be interrupted if the stop key is pressed or after 60
seconds without any further input.

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NOTE

If the runtime is changed during an active centrifugation run, the time that
has already elapsed will not be taken into consideration. The centrifuge will
perform a complete run with the new runtime.



6.3.2.1 Speed / relative centrifugal force (RCF)

The set speed of the centrifuge is displayed in the upper area of the
Speed/RCF field. The actual value is displayed right below. The speed is
stated as the number of revolutions per minute (min-1 = rpm) and the RCF
values as a multiple of the gravitational acceleration (x g). The values are
interdependent (see chapter 2.2.2.1 - "Speed, radius, and relative
centrifugal force"). The maximum speed/RCF values depend on the rotor
that is used.

The parameters speed and RCF can be changed during the centrifugation.



6.3.2.2 Runtime

Fig. 11: Setting the speed value or the RCF value

The set runtime is displayed in the upper section of this field, with the
remaining runtime shown below. The runtime is counted down from the set
value, starting with the start of the centrifuge and ending with the start of
the deceleration phase. The maximum runtime is 99 h:59 min:59 sec. As of
59 min:59 sec, the unit switches from "h:min" to "min:s".

The parameter runtime can be changed during the centrifugation.

Fig. 12: Setting the runtime, here in the time unit "min:s"

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Runtime as of the set speed

If the runtime is to be counted as of the moment when the set speed is
reached, the symbol (see the illustration) behind the set speed value must
be activated:

• Select the clock symbol with the cursor and confirm the selection. The
symbol and the bar under the symbol start to flash.

• Activate the function by turning the function knob. The symbol remains
displayed in a permanent manner and the bar continues to flash.

• Further turning of the function knob will deactivate the function. In this
case, the symbol disappears but the bar continues to flash.

• Press the function knob in order to activate the desired setting. The bar
remains visible as long as the cursor is placed on the symbol.

Fig. 13: The function "Runtime as of the set speed" is activated

Continuous run

During the continuous run, the runtime of the centrifuge is unlimited and
must be stopped manually. The centrifuge accelerates during the
continuous run until the set speed is reached.

• Select the "Time" field and press the function knob. The indication "set"
flashes in the activated status.

• Turn the function knob from the time 0:00:10 anti-clockwise or from the
time 99:59:59 clockwise. The indication "Cont" will be displayed in the
"Time" field. During the centrifugation run, the elapsed time will be
displayed.

• Deactivate the continuous run by pressing the stop key or by entering a
specific runtime.

Fig. 14: Indication “Cont” during a continuous run

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CAUTION

Unmoved air in the rotor chamber distorts the measuring and control
behavior and causes the compressor to freeze over. At temperatures
below 0°C, aqueous liquids will freeze, making sedimentation impossible.

• Ensure that the rotor temperature will not fall below 0 °C when the rotor
is at a standstill!

Short run

A short run can be started if no run is active.

• Keep the start key pressed during the short run.
During the short run, the centrifuge accelerates with the acceleration curve

9 (maximum) until the maximum permissible speed of the rotor is reached.
The runtime is counted and in the "Time" field the indication "Short" is
displayed.

When the start key is released, the centrifuge decelerates to a standstill
based on the maximum deceleration curve.

When the short run is completed, the original parameters (curves, runtime,
and final speed) are restored and displayed.

Fig. 15: Indication “Short” during a short run

6.3.2.3 Temperature ("Temp")

The set value is displayed in the upper area of the field and the actual
sample temperature is displayed in the lower area. Temperatures between

-10 °C and +60 °C can be preselected.

Fig. 16: Setting the temperature

Temperature control

Depending on the substances to be centrifuged, it may make sense to
perform a temperature control of the centrifuge. The temperature control
prevents the temperature-controlled samples in the non-temperature­controlled centrifuge from heating or cooling to an inadmissible
temperature.

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NOTE

The automatic lid opening function ("AutoOpen") is suppressed after a
temperature control run in order to prevent the system from reheating or
cooling down.

NOTE

If the "Rapid Temp" program is used, the temperature of the unloaded
aluminium bucket will be displayed. If samples, which have not been
precooled, are placed into the buckets after a ―Rapid Temp‖ run, the
displayed temperature will deviate from the actual sample temperature.

Program "Rapid Temp"

The centrifuge has a special "Rapid Temp" program that brings the
centrifuge rapidly to a specified temperature under defined conditions.

• Select the program with the aid of the menu item "run Prog" (see
chapter 6.3.3.2 - "Loading a program"). The speed display shows ⅓ of
the maximum rotor speed, the deceleration (brake) and acceleration
curves correspond to curve 9 and the runtime field indicates "Cont"
(continuous run). "Rapid Temp" will flash next to the ―run‖ display.

Fig. 17: "Rapid Temp" program

• Press the start button in order to start the temperature control process.

• During the temperature control process, only the set temperature can

be modified.

The "Rapid Temp" program will be stopped under the following conditions:

• The set value is reached. The program will stop with a sound signal if
this function is activated.

• The stop button is pressed. The program will be stopped prematurely.

• A parameter is changed (except for the temperature and

rotors/buckets). In this case, the program will be aborted.

• A short run is started. In this case, the program will also be aborted.

After the stop, the previous program will be reloaded or the changed
parameters will be adopted as the new settings.

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NOTE

The rotor selection can only be changed when the centrifuge is at a
standstill.




6.3.2.4 Rotor selection

This field shows the rotor that is currently being used.

• Select the "Rotor" field and confirm the selection. The indication "set"

• Select the desired rotor. If there are rotors with several different types of

• Confirm the input. The selected rotor or rotor/bucket combination will be

flashes in the activated status.

possible buckets, the various combinations will be displayed one after
the other.

adopted.

Fig. 18: Preselection of a rotor or a rotor/bucket combination

Automatic rotor identification system

If the centrifuge is equipped with an automatic rotor identification system,
the input mode will be activated automatically if the system detects a
different rotor with several different types of possible buckets than the rotor
that is set. The bucket that is displayed is the bucket with the lowest
maximum speed. You can only select different types of buckets for the
identified rotor. If the input mode is aborted, this bucket will be stored
nonetheless.

This prevents the maximum permissible speed from being exceeded.

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6.3.2.5 Acceleration and deceleration curves

Acceleration

This function is used to select an acceleration curve. The system offers 10
fixed, programmed acceleration curves (curves 0-9).

Brake

This function is used in order to select a curve that decelerates the
centrifuge to a standstill. Deceleration curves are inverted images of the
acceleration curves and are labelled with identical numbers. Deceleration
curve no. 0 represents a brakeless deceleration.



Fig. 19: Preselection of a curve; here: preselection of an acceleration curve

6.3.2.6 Automatic lid opening function ("AutoOpen")

The automatic lid opening function must be activated so that the lid opens
automatically at the end of the operation.

In order to activate the automatic lid opening function:

• Select the "Auto Open" symbol with the cursor and confirm the
selection. The symbol and the bar under the symbol start to flash.

• Activate the function by turning the function knob. The symbol remains
displayed in a permanent manner and the bar continues to flash.

• Turning the function knob further will deactivate the function. In this
case, the symbol disappears but the bar continues to flash.

• Press the function knob in order to activate the desired setting. The bar
remains visible as long as the cursor is placed over the symbol.

Fig. 20: The automatic lid opening function "Auto Open" is activated

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

6.3.2.7 Sound signal ("Buzzer")

This function is used to set an acoustic warning signal that sounds at the
end of the centrifugation run and also in the event of an imbalance or error
message.

In order to activate the sound signal:

• Select the "Buzzer" symbol with the cursor and confirm the selection.
The symbol and the bar under the symbol start to flash.

• Activate the function by turning the function knob. The symbol remains
displayed in a permanent manner and the bar continues to flash.

• Turning the function knob further will deactivate the function. In this
case, the symbol disappears but the bar continues to flash.

• Press the function knob in order to activate the desired setting. The bar
remains visible as long as the cursor is placed over the symbol.

Fig. 21: The sound signal “Buzzer” is activated

6.3.2.8 Program lock ("ProgLock")

When the program lock is active, it is impossible to save any new
programs. In this case, the function "save program" is disabled.

In order to activate the program lock:

• Select the "ProgLock" symbol with the cursor and confirm the selection.
The symbol and the bar under the symbol start to flash.

• Activate the function by turning the function knob. The symbol remains
displayed in a permanent manner and the bar continues to flash.

• Turning the function knob further will deactivate the function. In this
case, the symbol disappears but the bar continues to flash.

• Press the function knob in order to activate the desired setting. The bar
remains visible as long as the cursor is placed over the symbol.

Fig. 22: The program lock “ProgLock” is activated

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WARNING

When the maximum number of cycles of the rotor or bucket is reached, the
parts must be replaced immediately for safety reasons (see chapter

3.6.7.1 - "Service life ").

6.3.2.9 Cycle display ("Cycles")

In order to activate the cycle display:

• Select the "Cycles" symbol with the cursor and confirm the selection.
The symbol is displayed and "set" flashes in front of the rotor display.

• All of the rotors and buckets can be selected by turning the function
knob. The cycles of the selected rotor and, if applicable, also of the
selected bucket are displayed.

• Press the function knob in order quit the cycle display.

Fig. 23: Cycle display for rotor 11650 and bucket 13421

Reaching the maximum number of cycles

When the maximum number of cycles is reached for a rotor or bucket, a
corresponding warning signal will be displayed every time that the
centrifuge is started: the start key, lid key, and entire display will flash.

Fig. 24: Display when the maximum number of cycles is reached (flashing)

When the start key is pressed, "CYCLES" will be displayed. The centrifuge
will not be started and the display will not return to its normal state until the
start key is pressed again.

The cycle display will be reset after the rotor and buckets have been
replaced by the service department of Sigma Laborzentrifugen GmbH (see
chapter 7.3 - "Service contact").

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6.3.2.10 Input lock

In order to prevent the centrifuge from being manipulated by unauthorised
persons, inputs can be disabled via the menu. Inputs via the keypad, i.e. for
starting or stopping a centrifugation run or for opening the lid, are enabled.

Activating a simple input lock:

• Position the cursor over the symbol " " in the lower right-hand corner
of the display.

As long as the symbol is displayed, the parameters of the centrifuge cannot
be changed.

Activating a permanent input lock:

• Press the start key three times and hold for approximately 2 seconds
when pressing it for the third time.

After the activation of this function, the padlock symbol flashes. The input
lock is activated.

• Proceed in the same manner in order to deactivate the permanent input
lock.



Fig. 25: "Padlock" symbol indicating an activated input lock

6.3.3 Program mode

A program contains all the data that are required for a centrifuge run.
Certain sedimentation results can be repeated under identical conditions.

Programs can be saved, loaded, executed, and edited when the centrifuge
is at a standstill.

A maximum of 50 programs can be stored under the numbers 1 - 50. The
rapid cooling program "Rapid Temp" does not occupy any storage location
and cannot be deleted. It is used to cool the centrifuge without any vessels.

"--" means that the values that are currently set are not a stored program.
The programs can be protected against unauthorised use, modification, or

deletion with the aid of an input lock (see chapter 6.3.2.10 - "Input lock").

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NOTE

This function is only available if the centrifuge is at a standstill.

NOTE

The functions "Auto Open", "Buzzer", "ProgLock", and "Cycles" cannot be
entered as part of a program.




6.3.3.1 Saving a program

• Enter the parameters that are to be included in the program.

• Select the menu item "save Prog" and confirm the selection. The

indication "save" flashes in the activated status.

• Select a random storage location from the program selection list. Free
storage locations are indicated by a flashing display. Any storage
locations that are already occupied will be overwritten during the saving
process.

• Save the program in the desired location.

The program is now saved.

Fig. 26: Saving a program

6.3.3.2 Loading a program

• Select the menu item "run Prog" and confirm the selection. The
indication "run" flashes in the activated status.

• Select the desired program and confirm the selection by pressing the
function knob.

The program is now loaded.



Fig. 27: Loading a program

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6.4 Switching the centrifuge off

• Open the centrifuge when it is not in use so moisture can evaporate.

• Switch the centrifuge off by pressing the mains power switch.

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NOTE

Error messages can be eliminated by pressing the lid key. The error itself
will not be eliminated, but the centrifuge can be operated again.

Type of error

Possible reason

Correction

No indication on the display

No power in the mains supply

Check fuse in the mains supply

Power cord is not plugged in

Plug in power cord correctly

Fuses have tripped

Reactivate temperature fuse (see
chapter 5.2.1 - "Type of connection")

Mains power switch off

Switch mains power switch on

Centrifuge cannot be started:
start key LED is not illuminated

Several possible causes

Power off/on. If the error occurs
again, contact service

Centrifuge cannot be started:
lid key LED flashes

The lid lock is not closed correctly

Open and close the lid. If the error
occurs again, contact service

Centrifuge decelerates during
operation

Brief mains power failure

Press start key in order to restart the
centrifuge

System error

Power off/on. If the error occurs
again, contact service

Centrifuge decelerates during
operation, imbalance message is
displayed

– Improper loading
– Centrifuge is inclined
– Drive problem
– Centrifuge was moved during run

Balance load and restart the
centrifuge. If the error occurs again,
contact service (see chapter 7.1.1 ­"Emergency lid release")

– Ungreased load- bearing bolts

Clean and grease load- bearing bolts

Lid cannot be opened

Lid lock has not released

Unlock the lid manually and contact
service

Lid seal sticks

Clean the lid seal and apply talcum
powder

Temperature value cannot be reached
(only for refrigerated centrifuges)

Condenser dirty (only air-cooled units)

Clean the condenser. If the error
occurs again, contact service



7 Malfunctions and error correction



7.1 General malfunctions

Error messages are displayed as "Error" followed by a code number. If the
acoustic signal is activated, it sounds when the error message is displayed.

• Eliminate the source of the problem (see table below).

• Acknowledge the error messages by pressing the lid key.

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WARNING

Do not unlock or open the lid unless the rotor is at a standstill.

7.1.1 Emergency lid release

In the event of a power failure, it is possible to manually open the centrifuge
lid.

• Switch off the mains power switch and disconnect the power cord from
the socket.

• Remove the plug (see figure, item 1) from the opening at the side panel,
e.g. with a screwdriver.

Fig. 28: Position of the opening for the emergency lid release

• The plug is connected with a string. Pull the string in order to release
the lid.

• Then, reinsert the stopper into the opening.

If the lid is opened via the emergency lid release system during a centrifuge
run, the centrifuge will be switched off immediately and decelerate in an
unbraked manner.

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Error no.

Kind of error

Measures

Note

1-9

System error

• Allow to slow down

• Power off/on

All these errors stop the
centrifuge or cause it to
decelerate brakeless

10-19

Speedometer error

• Allow to slow down

• Power off/on

20-29

Motor error

• Power off

• Ensure ventilation

30-39

EEPROM error

• Allow to slow down

• Power off/on

With error 34, 35, and 36, the
centrifuge will stop;
with error 37 and 38 only an
error message will be given

40-45

Temperature error
(only for refrigerated centrifuges)

• Allow to slow down

• Power off

• Allow to cool down

• Provide better ventilation (only

air cooled centrifuges)

• Provide sufficient water
throughput (only water cooled
centrifuges)

46-49

Imbalance error (only for centrifuges
with imbalance monitoring system)

• Allow to slow down

• Power off

• Eliminate the imbalance

50-59

Lid error

• Press lid key

• Close lid

• Remove foreign matter from

the opening of the lid lock
device

With error 50 and 51, the
centrifuge will stop

60-69

Process error

• Allow to slow down

• Power off/on

With error 60, the message
"power failure during run"will
be displayed, with error 61,
the message "stop after
power on" will be displayed

70-79

Communication error

• Allow to slow down

• Power off/on

80-89

Parameter error

• Power off

• Allow to cool down

• Provide for better ventilation

With error 83, error message
only

90-99

Other errors

• Check connections

• Provide sufficient water

throughput (only water cooled
centrifuges)

NOTE

If it is impossible to eliminate the errors, contact the service!



7.2 Table of error codes

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NOTE

• If you would like to utilise our service, please state the type of your
centrifuge and its serial number.



7.3 Service contact

In the event of queries, malfunctions, or spare part enquiries:

From Germany:

Contact
Sigma Laborzentrifugen GmbH

An der Unteren Söse 50
37520 Osterode (Germany)
Tel. [phone]
E-mail: [e-mail]

Outside Germany:

Contact our agency in your country. All agencies are listed at
www.sigma-zentrifugen.de  [Sales Partners]

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CAUTION

If corrosion or other damage occurs due to improper care, the
manufacturer cannot be held liable or subject to any warranty claims.

WARNING

Take suitable precautions for your own safety if there is a risk of toxic,
radioactive, or pathogenic contamination.



8 Maintenance and service

The centrifuge, rotor, and accessories are subject to high mechanical
stress. Thorough maintenance performed by the user extends the service



life and prevents premature failure.

• Use soap water or other water-soluble, mild cleaning agents with a pH
value between 6 and 8 for cleaning the centrifuge and accessories (see
also chapter 8.2 - "Sterilisation and disinfection of the rotor chamber
and accessories").

• Avoid corrosive and aggressive substances.

• Do not use solvents.

• Do not use agents with abrasive particles.

• Do not expose the centrifuge and rotors to intensive UV radiation or

thermal stress (e.g. by heat generators).

8.1 Maintenance



8.1.1 Centrifuge

• Unplug the mains power plug before cleaning.

• Carefully remove all liquids, including water and particularly all the

solvents, acids, and alkaline solutions from the rotor chamber using a
cloth in order to avoid damage to the motor bearings.

• If the centrifuge has been contaminated with toxic, radioactive, or
pathogenic substances, clean the rotor chamber immediately with a
suitable decontamination agent (depending on the type of
contamination).

• After every cleaning process, grease the motor shaft slightly with a
small amount of heavy-duty grease for load-bearing bolts (part no.

71401) and distribute the grease with a cloth so that it forms a thin
layer.

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CAUTION

For the care of the accessories, special safety measures must be
considered as these are measures that will ensure operational safety at
the same time!

CAUTION

Do not clean the accessories in a dishwasher!

Cleaning in a dishwasher removes the anodised coating; the result is
cracking in areas that are subject to stress.



8.1.1.1 Condenser (only refrigerated centrifuges with an air-cooled refrigeration system)

In order to cool the refrigerant that is compressed by the refrigeration unit,
centrifuges with an air-cooled refrigeration system use a lamellar
condenser. It is cooled by air.

Dust and dirt obstruct the cooling flow of air. The dust on condenser pipes
and lamellas reduces the heat exchange and thus the performance of the
refrigeration unit.

This is why the installation site should be as clean as possible.

• Check the condenser at least once a month for dirt and clean it if
necessary.

• If you have any queries, please contact service (see chapter 7.3 -



8.1.2 Accessories

"Service contact").

• Immediately rinse off the rotor, buckets, or accessories under running
water if they have come into contact with any liquids that may cause
corrosion. Use a brush for test tubes in order to clean the bores of angle
rotors. Turn the rotor upside down and allow it to dry completely.

• Clean the accessories outside the centrifuge once a week or preferably
after each use. Adapters should be removed, cleaned and dried.

• If the rotors or accessories have been contaminated with toxic,
radioactive, or pathogenic substances, clean them immediately with a
suitable decontamination agent (depending on the type of
contamination). Take suitable precautions for your own safety if there is
a risk of toxic, radioactive, or pathogenic contamination.

• Dry the accessories with a soft cloth or in a drying chamber at



approx. 50°C.

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WARNING

Plastic accessories must not be greased!

8.1.2.1 Plastic accessories

The chemical resistance of plastic decreases with rising temperatures (see
chapter 11.5 - "Resistance data").

• If solvents, acids, or alkaline solutions have been used, clean the plastic



accessories thoroughly.

8.1.3 Rotors, buckets and carriers

Rotors, buckets and carriers are produced with the highest precision, in
order to withstand the permanent high stress from high gravitational fields.

Chemical reactions as well as stress-corrosion (combination of oscillating
pressure and chemical reaction) can affect or destroy the metals. Barely
detectable cracks on the surface can expand and weaken the material
without any visible signs.

• Check the material regularly (at least once a month) for
– cracks
– visible damage of the surface
– pressure marks
– signs of corrosion
– other changes.

• Check the bores of the rotors and multiple carriers.

• Replace any damaged components immediately for your own safety.

• After every cleaning process, grease the rotor tie-down screw slightly

with a small amount of heavy-duty grease for load-bearing bolts (part
no. 71401) and distribute the grease with a cloth so that it forms a thin



layer.

8.1.4 Microhaematocrit rotor

• Remove the microhaematrocrit rotor for cleaning.

• Wipe the centrifuge chamber clean.

• Replace the rubber ring in the event of wear or glass breakage (part no.

16001 for rotor 11409).

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NOTE

The load-bearing bolts of the rotor must not be greased if plastic buckets
are used!



8.1.5 Load-bearing bolts

Plastic bucket

Aluminium buckets

Only greased load-bearing bolts ensure a uniform swing-out of the buckets
and, therefore, the smooth operation of the centrifuge. Load-bearing bolts
that are insufficiently greased may cause the centrifuge to stop due to an
imbalance.

• Clean the load-bearing bolts and bucket groove in order to remove the
old grease.

• Apply a small amount of heavy-duty grease for load-bearing bolts (ref.
no. 71401, see the following picture) to both load-bearing bolts of a
bucket.

Fig. 29: Sufficient quantity of grease for one bolt

• Install the bucket and swing it manually back and forth once in order to
distribute the grease.

• Repeat this process with all the other buckets.

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CAUTION

In the case of glass breakage, immediately remove all glass particles (e.g.
with a vacuum cleaner). Replace the rubber cushions since even thorough
cleaning will not remove all glass particles.

DANGER

If dangerous materials (e.g. infectious and pathogenic substances) are
used, the centrifuge and accessories must be disinfected.



8.1.6 Glass breakage

Glass particles will damage the surface coating (e.g. anodising) of the
buckets, which will then lead to corrosion.

Glass particles in the rubber cushions of the buckets will cause glass
breakage again.

Glass particles on the pivot bearing of the load- bearing bolts prevent the
buckets and carriers from swinging evenly, which will cause an imbalance.

Glass particles in the rotor chamber will cause metal abrasion due to the
strong air circulation. This metal dust will not only pollute the rotor chamber,
rotor, and materials to be centrifuged but also damage the surfaces of the
accessories, rotors, and rotor chamber.

In order to completely remove the glass particles and metal dust from
the rotor chamber:

• Grease the upper third of the rotor chamber with e.g. Vaseline.

• Then, let the rotor rotate for a few minutes at a moderate speed

• Remove the grease with the glass and metal particles with a cloth.



• If necessary, repeat this procedure.

(approx. 2000 rpm). The glass and metal particles will now collect at the
greased part.

8.2 Sterilisation and disinfection of the rotor chamber and
accessories

• Use commercially-available disinfectants such as, for example,
Sagrotan, Buraton, or Terralin (available at chemist’s shops or
drugstores).

• The centrifuge and the accessories consist of various materials. A
possible incompatibility must be considered.

• Before using cleaning or decontamination agents that were not
recommended by us, contact the manufacturer to ensure that such a
procedure will not damage the centrifuge.

• For autoclaving, consider the continuous heat resistance of the
individual materials (see chapter 8.2.1 - "Autoclaving").

Please contact us if you have any queries (see chapter 7.3 - "Service
contact").

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NOTE

It cannot be excluded that plastic parts, e.g. lids or carriers, may deform
during autoclaving.

Accessories

Max. temp.

(°C)

Min. time

(min)

Max. time

(min)

Max. cycles

Aluminium buckets

134-138

3 5 -

Aluminium rotors

134-138

3 5 -

Glass tubes

134-138

3

40

-

Polyallomer / polycarbonate rectangular carriers

115-118

30

40

-

Polyallomer / polycarbonate round carriers

115-118

30

40

-

Polyamide buckets

115-118

30

40

10

Polycarbonate / polyallomer lids for angle rotors

115-118

30

40

20

Polycarbonate tubes

115-118

30

40

20

Polyphenylsulfone (PPSU) caps for buckets

134-138

3 5 100

Polypropylene balance adapter for blood-bag systems

115-118

30

40

n.s.

Polypropylene copolymer tubes

115-121

30

40

20

Polypropylene rectangular carriers

115-118

30

40

-

Polypropylene rotors

115-118

30

40

20

Polypropylene round carriers

115-118

30

40

-

Polysulfone caps for buckets

134-138

3 5 100

Polysulfone lids for angle rotors

134-138

3 5 100

Rubber adapters

115-118

30

40

-

Stainless-steel balance weight for blood-bag systems

121

30

30

n.s.

Teflon tubes

134-138

3 5 100



8.2.1 Autoclaving

The service life of the accessories essentially depends on the frequency of
autoclaving and use.

• Replace the accessories immediately when the parts show changes in
colour or structure or in the occurrence of leaks etc.

• During autoclaving, the caps of the tubes must not be screwed on in
order to avoid the deformation of the tubes.

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DANGER

In the event of service work that requires the removal of the panels, there
is a risk of electric shock or mechanical injury. Only qualified specialist
personnel is authorised to perform this service work.

NOTE

• If you would like to utilise our service, please state the type of your
centrifuge and its serial number.



8.3 Service

The centrifuge is subject to high mechanical stress. In order to be able to
withstand this high level of stress, high-quality components were used
during the production of the centrifuge. Nevertheless, wear cannot be
excluded and it may not be visible from the outside. Especially the rubber
parts that are – among other things – part of the motor suspension, are
subject to ageing.

This is why we recommend having the centrifuge checked by the
manufacturer during an inspection once per year in the operating state and
once every three years in the dismantled state. Rubber parts should be
replaced after three years.

Information and appointments:

In Germany:

Contact
Sigma Laborzentrifugen GmbH
An der Unteren Söse 50
37520 Osterode (Germany)
Tel. [phone]
E-mail: [e-mail]

Outside Germany:

Contact our agency in your country. All agencies are listed at
www.sigma-zentrifugen.de  [Sales Partners]

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NOTE

We will return the part/unit if no declaration of decontamination is provided!

NOTE

If the form does not include a description of the malfunction, neither a
refund nor a credit note can be issued. In this case, we reserve the right to
return the part/unit to you at your expense.



8.4 Return of defective centrifuges or parts

Although we exercise great care during the production of our products, it
may be necessary to return a unit or accessory to the manufacturer.

In order to ensure the quick and economical processing of returns of
centrifuges, spare parts, or accessories, we require complete and extensive
information concerning the process. Please fill in the following forms
completely, sign them, enclose them with the return package, and send
them together with the product to:

Sigma Laborzentrifugen GmbH
An der Unteren Söse 50
37520 Osterode (Germany)

1. Declaration of decontamination
As a certified company and due to the legal regulations for the
protection of our employees and of the environment, we are obliged to
certify the harmlessness of all incoming goods. For this purpose, we
require a declaration of decontamination.

• The form must be filled in completely and signed by authorised and

specialised personnel only.

• Affix the original form in a clearly visible manner to the outside of the

packaging.

2. Form for the return of defective parts
This form is for the product-related data. They facilitate the assignment,
and they enable the quick processing of the return. If several parts are
returned together in one packaging, please enclose a separate problem
description for every defective part.

• A detailed problem description is necessary in order to perform the

repair quickly and economically.

• Upon request, we will prepare and submit to you a cost estimate
prior to performing the repair. Please confirm such cost estimate
within 14 days. If the cost estimate has still not been confirmed after
4 weeks, we will return the defective part/unit. Please note that you
must bear the incurred costs.

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NOTE

The defective part/unit must be packaged in a transport-safe manner.
Please use the original packaging for the unit, if at all possible.

If the product is dispatched to us in unsuitable packaging, you will be
charged the cost for returning it to you in new packaging.

The forms can be downloaded online from
www.sigma-zentrifugen.de  [Service]  [Overhaul and repair].

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In accordance with the directive 2002/96/EC, SIGMA centrifuges are
marked with the symbol shown to the left. This symbol means that it is not
permissible to dispose of the unit among household waste.

• You can return these centrifuges free of cost to
Sigma Laborzentrifugen GmbH.

• Ensure that the unit is decontaminated. Fill in a declaration of
decontamination (see chapter 8.4 - "Return of defective centrifuges or
parts").

• Comply with any other applicable local rules and regulations.



9 Disposal



9.1 Disposal of the centrifuge



9.2 Disposal of the packaging

• Use the packaging to return the centrifuge for disposal or

• dispose of the packaging, after having separated the individual

materials.

• Comply with all local rules and regulations.

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Manufacturer

Sigma Laborzentrifugen GmbH
An der Unteren Söse 50
37520 Osterode (Germany)

Type:

2-16KHL

Connection requirements
Electr. connection:

Protection class:
IP code:

see name plate
I
20

Connected load (kVA):
Power consumption (kW):
Max. current consumption (A):
Input fuse (AT):

1.01

0.95

4.5 (at 220-240 V / 50/60 Hz)

6.0 (at 220-240 V / 50/60 Hz)

temperature fuses

Performance data
Max. speed (rpm):

Max. capacity (ml):
Max. gravitational field (x g):
Max. kin. energy (Nm):

15,300
400
21,913
9,962

Other parameters
Time range:

Temperature range:
Storage locations:

10 sec – 99 h 59 min,
short run, continuous run

-10°C to +60°C

50

Physical data
Height (mm):

Height with open lid (mm):
Width (mm):
Depth (mm):
Weight (kg):

310
705
550
570
60

Noise level (dB(A)):

65 (at maximum speed)

Refrigerant data
Refrigerant:

Filling quantity (kg):
Pressure (bar):
CO2 equivalent (t):

R134a

0.255

23

0.332



10 Technical data

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10 Technical data

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NOTE

At a nominal voltage of 100V or 200V, a tolerance of +10% / -5% applies.



10.1 Ambient conditions

• The figures are valid for an ambient temperature of +23°C and a
nominal voltage  10 %. The minimum temperature is ≤ +4°C and
depends on the rotor type, speed, and ambient temperature.

• For indoor use only.

• Allowable ambient temperature +5°C to +35°C.

• Max. allowable relative humidity of air 80% from 5°C up to 31°C with a

linear decrease to 67% relative humidity of air at 35°C.



• Maximum altitude 2,000 m above sea level.

10.2 Technical documentation

For environmental reasons, the comprehensive technical documentation of
the centrifuge (e.g. circuit diagrams) and the safety data sheets of the
manufacturers of refrigerants and lubricants are not attached to this
documentation.

You can order these documents from our service department.

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

11 Appendix



11.1 Range of accessories

The complete list of accessories can be downloaded from

11.1.1 Rotor radii

www.sigma-zentrifugen.de.

The information in the accessories table concerning the radius refers to the
values of the respective rotor as shown below. The radius calculation is
described in chapter 2.2.2.1 - "Speed, radius, and relative centrifugal
force".

Fig. 30: Minimum and maximum radius of an angle rotor

Fig. 31: Minimum and maximum radius of an swing-out rotor

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Fig. 32: Microhaematocrit rotor

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

11.2 Speed-gravitational-field-diagram

Fig. 33: Speed-gravitational-field-diagram

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Linear curve no.

Slope

0

4 [rpm/sec]

1

6 [rpm/sec]

2

8 [rpm/sec]

3

17 [rpm/sec]

4

25 [rpm/sec]

5

33 [rpm/sec]

6

50 [rpm/sec]

7

100 [rpm/sec]

8

200 [rpm/sec]

9

1.000 [rpm/sec]



11.3 Acceleration and deceleration curves

Linear curves are numbered in the direction of increasing acceleration
(from right to left).

The deceleration curves are inverted images of the acceleration curves and
are assigned the same numbers. An exception is curve 0. It decelerates
brakeless (spin-out).

In general, the runtime, until the set speed is reached, depends on the
moment of inertia of the rotor.

Linear curves

The slope of the fixed acceleration curves defines the time that is required
to accelerate the rotor by 1,000 rpm.

Curve 9 is a special case compared to the other curves. The centrifuge
accelerates with maximum power. The runtime, until the set speed is
reached, depends solely on the moment of inertia of the rotor.

Fig. 34: Slope of linear curves

Fig. 35: Diagram of linear curves

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Rotor /
bucket

Cycles

Service life

("Exp.Date")

Autoclaving Suitable for centrifuge Remarks

9100 15,000

4-15C, 4K15C, 4-16, 4-16S,
4-16K, 4-16KS, 6-15, 6K15,
6-16, 6-16K

without engraving, only
"spincontrol professional"
and "spincontrol S"

11026 7 years

1-14, 1-14K

11805 10 years*

8K, 8KB, 8KS, 8KBS

*in combination with
bucket 13850 or 13860

12033 5 years

1-16 Edition, 1-16K Edition

12082 7 years

1-14, 1-14K

12083 7 years

1-14, 1-14K

12084 7 years

1-14, 1-14K

12085 7 years

1-14, 1-14K

12092 5 years 20x

1-14, 1-14K

12093 5 years 20x

1-14, 1-14K

12094 5 years 20x

1-14, 1-14K

12096 5 years 20x

1-14, 1-14K

12097 5 years 20x

1-14, 1-14K

12101 5 years 20x

1-15, 1-15K, 1-15P, 1-15PK

12124 5 years 20x

1-15, 1-15K, 1-15P, 1-15PK

12126 5 years 20x

1-15, 1-15K, 1-15P, 1-15PK

12134 5 years 20x

1-16, 1-16K

12135 5 years 20x

1-16, 1-16K

12137 5 years 20x

1-16, 1-16K

12500 7 years

6-15, 6K15, 6-16, 6-16K

12600 7 years

6-16S, 6-16KS

13218 20,000

4-16, 4-16S, 4-16K, 4-16KS,
6-16, 6-16S, 6-16K, 6-16KS

13296 35,000 5 years 10x

2-6, 2-6E, 2-7,
2-16P, 2-16KL, 2-16KHL

13299 5 years 10x

2-6, 2-6E, 2-7,
2-16P, 2-16KL, 2-16KHL

13635 25,000

6-16, 6-16K, 6-16S, 6-16KS

13650 20,000

4-5L, 4-16S, 4-16KS,
4-16KHS, 6-16S, 6-16HS,
6-16KS, 6-16KHS

13845 20,000

8K, 8KB, 8KS, 8KBS

13850 10,000 10 years*

8K, 8KB, 8KS, 8KBS

*in combination with
rotor 11805

13860 15,000 10 years*

8K, 8KB, 8KS, 8KBS

*in combination with
rotor 11805

13864 1,000

8K, 8KB, 8KS, 8KBS without engraving

13865 1,000

8K, 8KB, 8KS, 8KBS without engraving

13866 1,000

8K, 8KB, 8KS, 8KBS without engraving

13867 2,500

8K, 8KB, 8KS, 8KBS without engraving

13870 5,000

8K, 8KB, 8KS, 8KBS



11.4 Table of the service life of rotors and accessories

• If no other data concerning the service life are engraved on the rotor or accessory, rotors and
buckets must be checked by the manufacturer after 10 years.

• If a specification concerning the maximum number of cycles and a specification concerning the
service life (i.e. a date) are provided, the specification that occurs first shall apply.

• After 50,000 cycles, rotors must be scrapped for safety reasons.

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NOTE

The data refer to resistance at 20°C.

- no data
1 resistant
2 practically resistant
3 partially resistant
4 not resistant

Concentration

High Density

Polyethylene

Polyamide

Polycarbonate

Polyoxymethylene

Polypropylene

Polysulfone

Polyvinyl chloride, hard

Polyvinyl chloride, soft

Polytetrafluorethylene

Acrylonitrile-butadiene-

caoutchouc

Aluminium

Medium

Formula

[%]

HDPE

PA

PC

POM

PP

PSU

PVC

PVC

PTFE

NBR

AL

Acetaldehyde

C2H4O

40

3 2 4 2 3 4 4 - 1 4 1

Acetamide

C2H5NO

saturated

1 1 4 1 1 4 4 - 1 - 1

Acetone

C3H6O

100

1 1 4 1 1 4 4 - 1 4 1

Acrylonitrile

C3H3N

100

1 1 4 3 3 4 4 4 1 4 1

Allyl alcohol

C3H6O

96

1 3 3 2 2 2 2 4 1 1 1

Aluminium chloride

AlCl3

saturated

1 3 2 4 1 - 1 - 1 1 4

Aluminium sulfate

Al2(SO4)3

10

1 1 1 3 1 1 1 1 1 1 1

Ammonium chloride

(NH4)Cl

aqueous

1 1 1 2 1 1 1 1 1 1 3

Ammonium hydroxide

NH3 + H2O

30

1 3 4 1 1 2 1 - 1 - 1

Aniline

C6H7N

100

1 3 4 1 2 4 4 4 1 4 1

Anisole

C7H8O

100

3 4 4 1 4 4 2 - 1 4 1

Antimony trichloride

SbCl3

90

1 4 1 4 1 - 1 - 1 - 4

Benzaldehyde

C7H6O

100

1 3 4 1 1 3 4 4 1 4 1

Benzene

C6H6

100

3 2 4 1 3 4 4 - 1 4 1

Boric acid

H3BO3

aqueous

1 3 1 2 1 - - - 1 1 1

Butyl acrylate

C7H12O2

100

1 2 4 2 3 4 4 4 1 - 1

Butyl alcohol, normal

C4H10O

100

1 1 2 1 1 2 2 4 1 1 1

Calcium chloride

CaCl2

alcoholic

1 4 2 3 1 - - 4 1 1 3

Carbon disulfide

CS2

100

4 3 4 2 4 4 4 4 1 3 1

Carbon tetrachloride
(TETRA)

CCl4

100

4 4 4 2 4 4 4 4 1 3 1

Chlorine

Cl2

100

4 4 4 4 4 4 4 4 1 - 3

Chlorine water

Cl2 x H2O

3 4 4 4 3 - 3 3 1 - 4

Chlorobenzene

C6H5Cl

100

3 4 4 1 3 4 4 4 1 4 1

Chloroform

CHCl3

100

3 3 4 4 3 4 4 4 1 4 3



11.5 Resistance data

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- no data
1 resistant
2 practically resistant
3 partially resistant
4 not resistant

Concentration

High Density

Polyethylene

Polyamide

Polycarbonate

Polyoxymethylene

Polypropylene

Polysulfone

Polyvinyl chloride, hard

Polyvinyl chloride, soft

Polytetrafluorethylene

Acrylonitrile-butadiene-

caoutchouc

Aluminium

Medium

Formula

[%]

HDPE

PA

PC

POM

PP

PSU

PVC

PVC

PTFE

NBR

AL

Chromic acid

CrO3

10

1 4 2 4 1 4 1 - 1 4 1

Chromic potassium
sulphate

KCr(SO4)2 x
12H2O

saturated

1 2 1 3 1 - 1 - 1 - 3

Citric acid

C6H8O7

10

1 1 1 2 1 1 1 1 1 1 1

Citric acid

C6H8O7

50

1 3 1 2 1 - - - 1 1 1

Copper sulphate

CuSO4 x 5H2O

10

1 1 1 1 1 1 1 1 1 1 4

Cyclohexanol

C6H12O

100

1 1 3 1 1 1 1 4 1 2 1

Decane

C10H22

100

- 1 2 1 3 - - - 1 2 1

Diaminoethane

C2H8N2

100

1 1 3 1 1 - 3 4 1 1 1

Diesel fuel — 100

1 1 3 1 1 - 1 3 1 1 1

Dimethyl formamide
(DMF)

C3D7NO

100

1 1 4 1 1 4 3 - 1 3 1

Dimethyl sulfoxide
(DMSO)

C2H6SO

100

1 2 4 1 1 4 4 - 1 - 1

Dimethylaniline

C8H11N

100

- 3 4 2 4 - - - 1 - 1

Dioxane

C4H8O2

100

2 1 4 1 3 2 3 4 1 3 1

Dipropylene glycol
(mono)methyl ether

C4H10O

100

3 1 4 1 4 4 4 4 1 - 1

Ethyl acetate

C4H8O2

100

1 1 4 1 1 4 4 4 1 4 1

Ethylene chloride

C2H4Cl2

100

3 3 4 1 3 4 4 4 1 - 1

Ferrous chloride

FeCl2

saturated

1 3 1 3 1 1 1 1 1 - 4

Formaldehyde solution

CH2O

30

1 3 1 1 1 - - - 1 2 1

Formic acid

CH2O2

100

1 4 3 4 1 3 3 1 1 2 1

Furfural

C5H4O2

100

1 3 3 2 4 - - - 1 4 1

Gasoline

C5H12 - C12H26

100

2 1 3 1 3 3 2 - 1 1 1

Glycerol

C3H8O3

100

1 1 3 1 1 1 1 2 1 1 1

Heptane, normal

C7H16

100

2 1 1 1 2 1 2 4 1 1 1

Hexane, n-

C6H14

100

2 1 2 1 2 1 2 4 1 1 1

Hydrogen chloride

HCl 5 1 4 1 4 1 1 1 - 1 2 4

Hydrogen chloride

HCl

concentrated

1 4 4 4 1 1 2 3 1 4 4

Hydrogen peroxide

H2O2 3 1 3 1 1 1 1 1 - 1 3 3

Hydrogen peroxide

H2O2

30

1 4 1 4 1 1 1 - 1 3 3

Hydrogen sulphide

H2S

10

1 1 1 1 1 1 1 3 1 3 1

Iodine, tincture of

I2 1 4 3 1 1 - 4 4 1 1 1

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- no data
1 resistant
2 practically resistant
3 partially resistant
4 not resistant

Concentration

High Density

Polyethylene

Polyamide

Polycarbonate

Polyoxymethylene

Polypropylene

Polysulfone

Polyvinyl chloride, hard

Polyvinyl chloride, soft

Polytetrafluorethylene

Acrylonitrile-butadiene-

caoutchouc

Aluminium

Medium

Formula

[%]

HDPE

PA

PC

POM

PP

PSU

PVC

PVC

PTFE

NBR

AL

Isopropyl alcohol

C3H8O

100

1 1 1 1 1 1 1 4 1 - 2

Lactic acid

C3H6O3

3

1 3 1 2 1 1 2 - 1 1 1

Magnesium chloride

MgCl2

10

1 1 1 1 1 1 1 1 1 1 1

Mercuric chloride

HgCl2

10

1 4 1 3 1 1 1 1 1 1 4

Mercury

Hg

100

1 1 1 1 1 1 1 3 1 1 3

Methyl acetate

C3H6O2

100

1 1 4 2 1 - 4 4 1 - 1

Methyl alcohol

CH4O

100

1 2 4 1 1 3 1 3 1 2 1

Methyl benzene

C7H8

100

3 1 4 1 3 4 4 4 1 4 1

Methyl ethyl ketone
(MEK)

C4H8O

100

1 1 4 1 1 4 4 4 1 4 1

Methylene chloride

CH2Cl2

100

4 3 4 3 3 4 4 4 1 - 1

Mineral oil — 100

1 1 1 1 1 1 1 - 1 1 1

Nitric acid

HNO3

10

1 4 1 4 1 1 1 - 1 4 3

Nitric acid

HNO3

100

4 4 4 4 4 - 4 - 1 4 1

Nitrobenzene

C6H5NO2

100

3 4 4 3 2 4 4 4 1 4 1

Oleic acid

C18H34O2

100

1 1 1 2 1 - 1 - 1 3 1

Oxalic acid

C2H2O4 x 2H2O

100

1 3 1 4 1 1 1 1 1 2 1

Ozone

O3

100

3 4 1 4 3 1 1 - 1 4 2

Petroleum — 100

1 1 3 1 1 1 1 3 1 1 1

Phenol

C6H6O

10

1 4 4 4 1 4 1 3 1 3 1

Phenol

C6H6O

100

2 4 4 4 1 3 4 3 1 3 1

Phosphoric acid

H3PO4

20

1 4 2 4 1 - - - 1 2 4

Phosphorus
pentachloride

PCl5

100

- 4 4 4 1 - 4 4 1 - 1

Potassium hydrogen
carbonate

CHKO3

saturated

1 1 2 1 1 - - - 1 - 4

Potassium hydroxide

KOH

30

1 1 4 3 1 1 1 1 1 - 4

Potassium hydroxide

KOH

50

1 1 4 3 1 1 1 1 1 - 4

Potassium nitrate

KNO3

10

1 1 1 1 1 - - - 1 1 1

Potassium
permanganate

KMnO4

100

1 4 1 1 1 - 1 - 1 3 1

Pyridine

C5H5N

100

1 1 4 1 3 4 4 4 1 4 1

Resorcinol

C6H6O2

5

1 4 2 3 1 4 2 - 1 - 2

Silver nitrate

AgNO3

100

1 1 1 1 1 1 1 1 1 2 4

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- no data
1 resistant
2 practically resistant
3 partially resistant
4 not resistant

Concentration

High Density

Polyethylene

Polyamide

Polycarbonate

Polyoxymethylene

Polypropylene

Polysulfone

Polyvinyl chloride, hard

Polyvinyl chloride, soft

Polytetrafluorethylene

Acrylonitrile-butadiene-

caoutchouc

Aluminium

Medium

Formula

[%]

HDPE

PA

PC

POM

PP

PSU

PVC

PVC

PTFE

NBR

AL

Sodium bisulphite

NaHSO3

10

1 1 2 4 1 - - - 1 1 1

Sodium carbonate

Na2CO3

10

1 1 1 1 1 - - - 1 - 3

Sodium chloride

NaCl

30

1 1 1 1 1 1 1 1 1 1 3

Sodium hydroxide

NaOH

30

1 1 4 1 1 1 1 1 1 2 4

Sodium hydroxide

NaOH

50

1 1 4 1 1 1 1 - 1 2 4

Sodium sulfate

Na2SO4

10

1 1 1 1 1 1 1 1 1 1 1

Spirits

C2H6O

96

1 1 1 1 1 1 1 3 1 - 1

Styrene

C8H8

100

4 1 4 1 3 - 4 4 1 4 1

Sulphuric acid

H2SO4 6 1 4 1 4 1 1 1 - 1 2 3

Sulphuric acid

H2SO4

fuming

4 4 4 4 4 4 4 4 1 4 3

Tallow — 100

1 1 1 1 1 - 1 1 1 1 1

Tetrahydrofuran (THF)

C4H8O

100

3 1 4 1 3 4 4 4 1 3 1

Tetrahydronaphthalene

C10H12

100

3 1 4 1 4 4 4 4 1 - 1

Thionyl chloride

Cl2SO

100

4 4 4 2 4 4 4 4 1 - 3

Tin chloride

SnCl2

10

1 4 2 2 1 - - - 1 1 4

Transformer oil

—

100

1 1 3 3 1 1 1 - 1 1 1

Trichloroethane

C2H3Cl3

100

3 3 4 2 4 4 4 4 1 4 4

Urea

CH4N2O

10

1 1 1 1 1 - - - 1 1 1

Urine — 100

1 1 1 1 1 - 1 1 1 - 2

Vinegar

C2H4O2

10

1 4 1 1 1 1 1 1 1 2 1

Vinegar

C2H4O2

90

1 4 4 4 1 3 1 4 1 - 1

Wax

—

100

- 1 1 1 - - - 1 - 1

Wines — 100

1 1 1 2 1 1 1 1 1 - 4

Xylene

C8H10

100

3 1 4 1 4 4 4 4 1 4 1

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11.6 EC declaration of conformity

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11.7 Declaration of conformity – China RoHS 2

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Index

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12 Index

A

Acceleration ............................................... 41

Acceleration curves .............................. 41, 66

Accessories, cleaning and care .................. 52

Acid................................................ 20, 51, 53

Activating the modification mode ................ 35

Adapters .............................................. 33, 52

Alkaline solutions ........................... 20, 51, 53

Ambient conditions ..................................... 62

Ambient temperature ................................. 62

Anodised coating ....................................... 52

Application examples ................................. 13

Autoclaving ................................................ 56

Automatic lid opening function ................... 41

AutoOpen .................................................. 41

B

Brake ......................................................... 41

Brief mains power failure ............................ 47

Bucket ........................................................ 33

Buckets, cleaning and care ........................ 53

Buzzer ....................................................... 42

C

Capacity ..................................................... 61

Capillary tubes ........................................... 31

Carrier ........................................................ 33

Carrier systems .......................................... 33

Carriers, cleaning and care ........................ 53

CE mark in compliance with the directive

2006/42/EC ............................................ 15

Centrifugation of infectious, toxic, radioactive,

or pathogenic substances ....................... 19

Centrifugation principle .............................. 13

Centrifugation with low capacity ................. 32

Centrifugation with vessels of various sizes 31

Centrifuge cannot be started ...................... 47

Centrifuge decelerates during operation .... 47

Centrifuge is inclined .................................. 47

Centrifuge was moved during run .............. 47

Centrifuge, cleaning and care .................... 51

Centrifuges

- definition ................................................. 9

Changes in colour ...................................... 56

Changes in structure .................................. 56

Chemical and biological safety ................... 19

Chemical resistance of plastic .................... 53

China RoHS 2 – Declaration of conformity . 75

Cleaning agents ......................................... 55

Cleaning the bores of angle rotors ............. 52

Cleaning the centrifuge .............................. 51

Closing the lid ................................ ...... 18, 28

CO2 equivalent ........................................... 61

Communication error ................................. 49

Condensation ............................................. 26

Condenser dirty ......................................... 47

Condenser, cleaning and care ................... 52

Connected load .......................................... 61

Contamination ...................................... 19, 52

Continuous heat resistance ........................ 55

Continuous run .......................................... 37

Control system ........................................... 34

Copyright ................................................... 10

Corrosion ................ 18, 21, 33, 51, 52, 53, 55

Cost estimate ............................................. 58

Cracking .................................................... 52

Cracks ....................................................... 53

Current consumption .................................. 61

Customer-provided fuses ........................... 27

Cycle display (Cycles) ................................ 43

Cycles ........................................................ 28

Cycles (cycle display) ................................ 43

D

Damage of the surface ............................... 53

Dangerous goods....................................... 20

Dangerous materials .................................. 55

Date of manufacture .................................. 12

Deceleration curves ............................. 41, 66

Declaration of conformity ..................... 10, 73

Declaration of conformity – China RoHS 2 . 75

Declaration of decontamination ............ 58, 60

Decontamination agent ........................ 52, 55

Deformation of tubes .................................. 56

Density ................................................. 12, 20

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Different service life of rotors and accessories

......................................................... 21, 67

Dimensions ................................................ 24

Direct hazard to the life and health ............. 16

Directive 2002/96/EC ................................. 60

Disinfectants .............................................. 55

Disinfection of the rotor chamber and

accessories ................................ ............ 55

Display ....................................................... 34

Disposal of the centrifuge ........................... 60

Disposal of the packaging .......................... 60

Documentation ........................................... 10

Drive problem ............................................ 47

E

Earth conductor check ............................... 22

EC declaration of conformity ................ 10, 73

EEPROM error ........................................... 49

Electrical connection .................................. 61

Electrical safety .......................................... 18

Emergency lid release ............................... 48

Error correction .......................................... 47

Error message ........................................... 47

Explanation of the symbols and notes ........ 16

Explosive substances ................................ 19

Imbalance monitoring system .................... 22

Importance of the operating manual ............. 9

Important information ................................. 16

Improper loading ........................................ 47

Infectious substances ................................ 55

Inflammable substances ............................ 19

Informal safety instructions ........................ 17

Initial start-up ................................ ............. 28

Input fuse ................................................... 61

Input lock ................................................... 44

Inspection by the manufacturer .................. 57

Installation of a rotor .................................. 28

Installation of accessories .......................... 31

Installation of angle rotors with a hermetic lid

............................................................... 29

Installation of rotors and accessories ......... 28

Installation of the microhaematocrit rotor ... 30

Installation site ........................................... 26

Insufficiently greased load-bearing bolts .... 54

Intended use ................................................ 9

Interrupting a centrifugation run ................. 35

Interrupting a deceleration process ............ 35

IP code ...................................................... 61

K

F

Filling quantity (refrigerant) ........................ 61

Fire preventions ......................................... 19

Form for the return of defective parts ......... 58

Functional and operating elements ............ 11

Fuses have tripped .................................... 47

G

General conditions ................................ ....... 9

Glass breakage .......................................... 55

Glass particles ........................................... 55

Gravitational field ....................................... 61

Grease for load-bearing bolts ............... 51, 54

H

Hazard warnings ........................ 9, 28, 29, 33

Heavy-duty grease for load-bearing bolts ... 54

Highly corrosive substances ...................... 18

I

Imbalance ............................................ 31, 33

Imbalance error .......................................... 49

Kinetic energy ...................................... 12, 61

L

Layout of the centrifuge.............................. 11

Leaks ......................................................... 56

Lid cannot be opened ................................ 47

Lid error ..................................................... 49

Lid lock device ........................................... 22

Lid lock has not released ........................... 47

Lid seal sticks ............................................ 47

Linear curves ............................................. 66

Loading a program ..................................... 45

M

Mains power switch off ............................... 47

Mains switch .............................................. 11

Mains voltage ............................................. 18

Maintenance .............................................. 51

Malfunctions and error correction ............... 47

Manual mode ............................................. 35

Manufacturer .............................................. 61

Marking of the unit ..................................... 15

Refrigerated Centrifuge with Heating System Sigma 2-16KHL

Index

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Translation of the original operating manual, part no. 0700802

Maximum number of cycles ....................... 43

Maximum speed for tubes .......................... 33

Measures in the event of hazards and

accidents ................................................ 23

Mechanical safety ...................................... 18

Microhaematocrit rotor, cleaning and care . 53

Mode of operation ...................................... 13

Motor error ................................ ................. 49

Motor shaft ..................................... 28, 29, 51

Multiple carrier ........................................... 33

N

Name plate .......................................... 11, 12

No indication on the display ....................... 47

No power in the mains supply .................... 47

Noise level ................................................. 61

Nominal voltage ......................................... 12

Notes on safety and hazards ....................... 9

Notes on transport ..................................... 24

O

Online download of forms .......................... 59

Opening the lid ........................................... 28

Operating personnel .................................. 17

Operating voltage....................................... 26

Operational safety ...................................... 52

Overseas shipping ..................................... 24

P

Packaging .................................................. 25

Parameter error ......................................... 49

Pathogenic substances .................. 19, 51, 55

Plastic accessories, cleaning and care ....... 53

Potential hazard to the life and health ........ 16

Potentially hazardous situation................... 16

Power consumption ............................. 12, 61

Power cord is not plugged in ...................... 47

Power supply ............................................. 26

Preselection of a curve............................... 41

Pressure (refrigerant) ................................. 61

Pressure marks .......................................... 53

Prevention of accidents ................................ 9

Problem description ................................... 58

Process error ............................................. 49

ProgLock program lock .............................. 42

Program "Rapid Temp" .............................. 39

Program lock (ProgLock) ........................... 42

Protection class ................................... 26, 61

Q

Quick stop .................................................. 35

Quitting the modification mode ................... 35

R

Radioactive substances ....................... 19, 51

Radius ....................................................... 14

Rapid Temp program ................................. 39

Reaching the maximum number of cycles .. 43

Refrigerant ................................ ................. 61

Refrigerant data ......................................... 61

Relative centrifugal force (RCF) ........... 14, 36

Removal of a rotor ............................... 29, 30

Removal of the transport safety device ...... 25

Remove glass particles and metal dust from

the rotor chamber ................................... 55

Responsibility of the operator ..................... 17

Return of centrifuges, spare parts, or

accessories ............................................ 58

Return of defective parts ............................ 58

Risk of electrical shock............................... 18

Rotor chamber ........................................... 51

Rotor monitoring system ............................ 22

Rotor radii .................................................. 63

Rotor selection ........................................... 40

Rotor tie-down screw ..................... 28, 29, 53

Rotor wrench ....................................... 28, 29

Rotors and accessories with a different

service life ........................................ 21, 67

Rotors for microtiter plate formats .............. 29

Rotors, cleaning and care .......................... 53

Runtime ..................................................... 36

Runtime as of the set speed ...................... 37

S

Safety area ................................................ 20

Safety devices ........................................... 22

Safety distance .............................. 18, 20, 26

Safety instructions ...................... 9, 28, 29, 33

Safety instructions for centrifugation .......... 20

Safety of rotors and accessories ................ 21

Safety, chemical and biological .................. 19

Safety, electrical ........................................ 18

Safety, mechanical ..................................... 18

Safety-conscious work ............................... 17

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Index

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Translation of the original operating manual, part no. 0700802

Saving a program....................................... 45

Scope of supply ......................................... 10

Selection, display, and modification of data 35

Serial number ................................ 12, 50, 57

Service ....................................................... 57

Service contact .......................................... 50

Service life ................................................. 51

Service life of rotors and accessories ... 21, 67

Service life of the accessories .................... 56

Service work .............................................. 57

Set-up and connection ............................... 26

Short run .................................................... 38

Solvents ......................................... 20, 51, 53

Sound signal (buzzer) ................................ 42

Spare part enquiries ................................... 50

Speed ...................................... 12, 14, 36, 61

Speed-gravitational-field-diagram .............. 65

Speedometer error ..................................... 49

Standards and regulations ......................... 10

Standstill monitoring system ...................... 22

Starting a centrifugation run ....................... 35

Sterilisation of the rotor chamber and

accessories ................................ ............ 55

Stopping of the centrifuge due to an

imbalance ............................................... 54

Storage and transport ................................ 24

Storage conditions ..................................... 24

Storage locations ....................................... 61

Stress-corrosion

see

corrosion ............................................. 53

Structural changes ................................ ..... 19

Supply voltage ........................................... 26

Switching the centrifuge off ........................ 46

Switching the centrifuge on ........................ 28

System check ............................................ 22

System error ........................................ 47, 49

T

Table of error codes ................................... 49

Table of rotors and accessories with a

different service life .......................... 21, 67

Technical data ........................................... 61

Technical documentation ........................... 62

Temperature .............................................. 38

Temperature control ................................... 38

Temperature error ...................................... 49

Temperature inside the rotor chamber ....... 22

Temperature monitoring system ................. 22

Temperature value not reached ................. 47

Thermal stress ........................................... 51

Time range................................................. 61

Toxic substances ................................. 19, 51

Transport safety device .............................. 25

Tubes ......................................................... 33

Type........................................................... 12

Type of connection ..................................... 26

Type of the centrifuge .................... 50, 57, 61

U

Ungreased load- bearing bolts ................... 47

Useful volume

- volume that is stated for the tube .......... 33

User interface ............................................ 34

UV radiation ......................................... 26, 51

V

Vents ......................................................... 26

Vessels ...................................................... 33

W

Warranty and liability .................................... 9

Wear .......................................................... 57

Weight ....................................................... 24

Work on the power supply system ............. 18