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ADVIA®2400 Chemistry System
Operator's Guide
073D0403-01 Rev. A, 2009-06
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The ADVIA® 2400 Chemistry system is for in vitro diagnostic use.
The information in this guide was correct at the time of release. However,
Siemens Healthcare Diagnostics continues to improve products and reserves the right to
change specifications, equipment, and maintenance procedures at any time without
notice.
If the system is used in a manner not specified by Siemens, the protection provided by
the equipment may be impaired. Observe all warning and hazard statements.
The ADVIA Chemistry system is manufactured in Japan for
Siemens.
THE UNIVERSAL RACK HANDLING SYSTEM IS MANUFACTURED IN GERMANY FOR
S
IEMENS.
RIGIN: JAPAN
O
Copyright and trademarks
ADVIA Centaur and ADVIA are trademarks of Siemens Healthcare Diagnostics.
Acrobat is a trademark of Adobe Systems Incorporated, San Jose, CA.
Clorox is a trademark of The Clorox Company, Oakland, CA.
Windows is a trademark of Microsoft Corporation.
© 2009 Siemens Healthcare Diagnostics Inc. All rights reserved.
No part of this manual or the products it describes may be reproduced by any means or
in any form without prior consent in writing from Siemens.
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Table of Contents
1 System Overview ...................................................................................................................................13
Operating principle ...................................................................................................................................13
Hardware Overview...................................................................................................................................14
Display and power panel..........................................................................................................................................15
Analyzer back view ..................................................................................................................................................15
Analyzer top view.....................................................................................................................................................16
Vertical Pumps.........................................................................................................................................................17
Horizontal Pumps ....................................................................................................................................................17
Workstation (front view) ...........................................................................................................................................18
PC (front view) .........................................................................................................................................................18
PC (back view).........................................................................................................................................................19
Sampling and analysis .............................................................................................................................................19
Sampling mechanisms.............................................................................................................................................19
Sampling operation .............................................................................................................................................19
Sample probe......................................................................................................................................................20
Sample pump ......................................................................................................................................................20
Clot detection...........................................................................................................................................................21
Reaction tray............................................................................................................................................................21
Reaction tray mixers ................................................................................................................................................22
Reaction tray wash mechanisms .............................................................................................................................22
Reaction tray washer operation ...............................................................................................................................24
Reaction tank...........................................................................................................................................................25
Sample tray..............................................................................................................................................................25
Sample tray .........................................................................................................................................................25
Sample tray operation .........................................................................................................................................26
Container types ...................................................................................................................................................26
SMP Pause .........................................................................................................................................................26
Spectrophotometer ..................................................................................................................................................27
Reagents ....................................................................................................................................................27
Reagent mechanisms ..............................................................................................................................................27
Reagent trays ..........................................................................................................................................................28
Reagent probes .......................................................................................................................................................29
Reagent pumps .......................................................................................................................................................29
Reagent tray operation ............................................................................................................................................30
Reagent container types .....................................................................................................................................30
RGT Pause button...............................................................................................................................................30
System Overview 3
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Sample aspiration and dilution mechanisms............................................................................................................30
Dilution tray mixer................................................................................................................................................31
Dilution tray mixer operation................................................................................................................................31
Dilution tray wash mechanisms...........................................................................................................................31
Dilution tray wash mechanism operation.............................................................................................................33
ISE (electrolyte analyzer)..........................................................................................................................34
Universal rack handler – functional description (optional) ..................................................................35
Software Overview .................................................................................................................................... 36
Startup window ........................................................................................................................................................36
Operation Panel.......................................................................................................................................................36
Buttons ................................................................................................................................................................37
Status and message boxes .................................................................................................................................37
Other tasks accessed from System(s) on the Operation Panel...........................................................................37
Menu Panel..............................................................................................................................................................38
Other tasks accessed from System(s) on the Menu Panel..................................................................................38
Description of software windows ............................................................................................................ 40
Request windows.....................................................................................................................................................40
Order Entry window.............................................................................................................................................40
Sample Log window ............................................................................................................................................40
Test Result Monitor window ................................................................................................................................41
Sample test status color codes ...........................................................................................................................41
Review/Edit window ............................................................................................................................................43
Reaction Monitor window ....................................................................................................................................43
RealTime Monitor window ...................................................................................................................................43
Print Report window*...........................................................................................................................................44
Test Select window*............................................................................................................................................44
Cup/Tube Assign window* ..................................................................................................................................44
Test Utilization Statistics window ........................................................................................................................44
Correlation window* ............................................................................................................................................45
Calibration windows.................................................................................................................................................45
View Calibration Curve window...........................................................................................................................45
Calibration/RBL History .......................................................................................................................................45
Sample Select window*.......................................................................................................................................46
Calibration Setup window* ..................................................................................................................................46
Maintenance windows..............................................................................................................................................46
System Startup/Shutdown Setting window..........................................................................................................46
User Maintenance window ..................................................................................................................................47
System Monitor window ......................................................................................................................................47
System Maintenance Monitor window.................................................................................................................47
Lamp Energy Monitor window .............................................................................................................................47
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ISE Operation window.........................................................................................................................................47
ISE Monitor window.............................................................................................................................................48
Manual Operation window...................................................................................................................................48
On-Line Monitor window......................................................................................................................................48
Error Report window* ..........................................................................................................................................49
Reagent windows ....................................................................................................................................................49
Reagent Inventory window..................................................................................................................................49
CTT Monitor window ...........................................................................................................................................49
Reagent Container Settings window ...................................................................................................................49
Active Test List window*......................................................................................................................................50
Reagent Information window* .............................................................................................................................50
Reagent Barcode Maintenance window*.............................................................................................................51
QC windows.............................................................................................................................................................51
ADVIA QC window ..............................................................................................................................................51
Real-time QC window..........................................................................................................................................52
Daily Precision Control window ...........................................................................................................................52
QC Cumulative window.......................................................................................................................................53
Control Data Setup window* ...............................................................................................................................53
Sample Select window*.......................................................................................................................................54
QC Sample Definition window* ...........................................................................................................................54
Setup windows.........................................................................................................................................................54
System Specification Settings window*...............................................................................................................54
Analytical Parameters (Chemistry) window*........................................................................................................55
System Test List window.....................................................................................................................................56
Analytical Parameters (Serum) window* .............................................................................................................56
Process Sequence window*................................................................................................................................57
Ratio Parameters window* ..................................................................................................................................57
Contamination Settings window*.........................................................................................................................57
Ctrl/Cal Sample Setup window* ..........................................................................................................................57
User Code Settings window*...............................................................................................................................58
New Test Definition window*...............................................................................................................................58
ISE Parameter Settings window* ........................................................................................................................58
Print Form Settings window* ...............................................................................................................................58
Online Settings window*......................................................................................................................................59
Alarm Buzzer Settings window* ..........................................................................................................................59
System Parameters Settings window*.................................................................................................................60
Reflex Test Settings window*..............................................................................................................................60
Other Tasks .............................................................................................................................................................60
Version Information window .................................................................................................................... 61
ADVIA o.p. ..........................................................................................................................................................61
System Overview 5
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Controller.............................................................................................................................................................61
Safety Message ..................................................................................................................................................61
Etc.......................................................................................................................................................................62
Memo ..................................................................................................................................................................62
Using online help ......................................................................................................................................62
Viewing the content .............................................................................................................................................63
2 Operating the System............................................................................................................................ 65
Start the System........................................................................................................................................65
Logging on ...............................................................................................................................................................65
Starting the system ..................................................................................................................................................65
Performing a system-assisted startup (Start set) .....................................................................................................66
Checking the analyzer .............................................................................................................................................67
Times required to perform prime, washes, and cell blank ...................................................................................71
Daily Operation.......................................................................................................................................... 72
Using workorders.....................................................................................................................................................72
Using host computer workorders.........................................................................................................................72
Creating workorders at the analyzer....................................................................................................................73
Creating multiple workorders...............................................................................................................................73
Deleting or changing test selectivity for multiple workorders ...............................................................................74
Creating a profile .....................................................................................................................................................75
Creating a load list ...................................................................................................................................................75
Loading patient samples.......................................................................................................................... 76
Loading patient samples on the STT .......................................................................................................................76
Loading patient samples on the optional universal rack handler..............................................................................77
Urgent samples and manual reruns are run from the onboard sampler (STT) ....................................................79
Starting the run ........................................................................................................................................................79
Running calibrators and reagent baselines (RBL)...............................................................................................80
Running control samples.....................................................................................................................................80
Running an interrupt (STAT) sample...................................................................................................................81
Monitoring an analysis while it is running.................................................................................................................82
Processing status................................................................................................................................................82
Sample information and system status................................................................................................................82
Monitoring samples loaded on the optional universal rack handler..........................................................................83
Using the Sample Log..............................................................................................................................................84
Viewing the sample log entries............................................................................................................................84
Searching the sample log....................................................................................................................................84
Deleting a specific sample log entry....................................................................................................................85
Deleting all sample log entries ............................................................................................................................85
Printing a list of the sample log entries................................................................................................................85
Exporting the sample log entries .........................................................................................................................86
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Reviewing the calibration results .............................................................................................................................86
Reviewing the sample results ..................................................................................................................................86
Reporting results......................................................................................................................................................86
Real-time reporting..............................................................................................................................................86
Batch transmission to a host computer ...............................................................................................................87
Printing sample data ................................................................................................................................................87
Batch Printing......................................................................................................................................................88
Shutting down the ADVIA Chemistry system ........................................................................................88
Examples of shutdown settings ...............................................................................................................................89
System available during the night .......................................................................................................................89
Automatic startup from the Sleep state ...............................................................................................................89
Automatic startup with analyzer power kept ON..................................................................................................89
Performing a system-assisted shutdown (Shutdown set) ........................................................................................90
Additional Operating Instructions........................................................................................................... 91
Switching Process Sequence and System Test List numbers.............................................................................91
Loading the reagent trays....................................................................................................................................91
Updating the Cumulative QC...............................................................................................................................91
Running automatic calibration ............................................................................................................................92
Using multiple reagent containers for the same test ...........................................................................................92
3 Calibration ..............................................................................................................................................93
Calibration overview ................................................................................................................................. 93
When to calibrate.....................................................................................................................................................93
Setting up the calibration .........................................................................................................................................93
Entering absolute or single-point (STD) calibration methods ..............................................................................93
Entering multi-standard calibration methods .......................................................................................................94
How to calibrate a multi-standard method ...........................................................................................................95
Running calibration ..................................................................................................................................................95
Reviewing the calibration .........................................................................................................................................96
4 Quality Control .......................................................................................................................................99
Quality control overview .......................................................................................................................... 99
When to run control samples ...................................................................................................................................99
Setting up quality control .....................................................................................................................................99
Running the control samples...............................................................................................................................99
ADVIA QC window.................................................................................................................................................100
Reviewing the control results at different windows ................................................................................................100
5 Maintenance .........................................................................................................................................101
Maintenance schedule............................................................................................................................101
Daily maintenance...................................................................................................................................103
Inspecting and cleaning the probes .......................................................................................................................103
System Overview 7
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Cleaning the probes using automatic advance probe motion ............................................................................103
Cleaning the probes using manual probe motion ..............................................................................................104
Probe cleaning procedure (optional method) ....................................................................................................106
Inspecting and cleaning the mixing rods and mixer wash cups .............................................................................107
Checking reagents and system solutions...............................................................................................................108
Inspecting and cleaning the reaction (WUD) and dilution (DWUD) cuvette washers ............................................. 108
Inspecting and cleaning the cuvette splash covers................................................................................................111
Inspecting and cleaning the probe wash cups .......................................................................................................112
Checking pumps for leaks......................................................................................................................................113
Checking the SP and DIP vertical pumps for leaks ...........................................................................................113
Checking other vertical pumps for leaks............................................................................................................114
Checking the horizontal pumps for leaks...........................................................................................................114
Performing the startup wash (WASH3)..................................................................................................................115
Performing the shutdown wash..............................................................................................................................117
Performing additional ISE electrode washes ....................................................................................... 118
Recording ISE slopes ............................................................................................................................................119
Weekly maintenance...............................................................................................................................119
Performing the weekly wash..................................................................................................................................119
Checking and replenishing the lamp coolant .........................................................................................................121
Checking lamp energy ...........................................................................................................................................122
Reading lamp energy data ................................................................................................................................123
Measuring cuvette blanks ......................................................................................................................................124
Cell blank measurement results.............................................................................................................................124
Cleaning the analyzer and rack handler exterior panels ........................................................................................125
Monthly maintenance .............................................................................................................................126
Cleaning the turntable interiors (STT/CTT and RTT) .............................................................................................126
Cleaning the inside of the STT/CTT housing.....................................................................................................126
Cleaning the inside of the reagent tray refrigerated housing .............................................................................127
Cleaning or replacing the wash solution reagent containers (47 – 50) ..................................................................127
Cuvette wash and cuvette conditioner usage....................................................................................................128
Cleaning and replenishing the dilution bottle .........................................................................................................128
Cleaning and replenishing the cuvette wash bottle................................................................................................129
Cleaning the chiller filter.........................................................................................................................................130
Every 2 months maintenance ................................................................................................................131
Cleaning the dilution tray cuvettes .........................................................................................................................131
Cleaning and replenishing the cuvette conditioner bottle.......................................................................................132
Every 3 months maintenance ................................................................................................................133
Replacing the lamp ................................................................................................................................................133
Washing the ISE electrodes lines ..........................................................................................................................135
Every 4 months maintenance ................................................................................................................137
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Cleaning the ancillary reagent bottle filters ............................................................................................................137
Cleaning the pure-water bottle filter .......................................................................................................................137
Replacing the reaction and dilution cuvettes..........................................................................................................140
As required maintenance .......................................................................................................................143
Backing up system files .........................................................................................................................................143
Restoring system files............................................................................................................................................144
Replacing the SPP, RPP1, and RPP2 probes .......................................................................................................144
Remove the probe.............................................................................................................................................145
Install a new probe.................................................................................................................................................146
Replacing DPP probes equipped with crash detection ..........................................................................................147
Removing the DPP probe..................................................................................................................................147
Install the new probe..............................................................................................................................................149
Priming the system ................................................................................................................................................150
Replenishing the RRV (reaction) bath oil bottle .....................................................................................................150
Preventive cleaning of the wash station lines ........................................................................................................151
Washing all the ISE lines .......................................................................................................................................155
Washing the lines ..............................................................................................................................................156
Rinsing the lines................................................................................................................................................156
Priming and initializing the ISE module .............................................................................................................157
Conditioning the ISE Na and K electrodes.............................................................................................................157
Replacing ISE electrodes.......................................................................................................................................158
Removing electrodes.........................................................................................................................................159
Installing electrodes...........................................................................................................................................159
Priming the ISEs ...............................................................................................................................................159
Calibrating the ISEs...........................................................................................................................................160
Storing the reference electrode .........................................................................................................................160
Cleaning the dilution bowl and waste-drain nozzle ................................................................................................161
Cleaning the dilution bowl .................................................................................................................................161
Cleaning the waste-drain nozzle .......................................................................................................................162
Maintaining the ISE unit after the dilution bowl and waste-drain nozzle are clean ............................................163
Recovering from a power failure ............................................................................................................................163
Preparing the system for an expected power outage ........................................................................................163
Preparing the system for power return (if power was unexpectedly lost while system was on).........................163
Recovering from an unexpected power outage (after power returns,
when power was unexpectedly lost while system was on) ...............................................................................164
6 Troubleshooting...................................................................................................................................166
7 File Management .................................................................................................................................. 168
Saving the error log to a test file ...........................................................................................................168
Capturing the error report:.................................................................................................................................168
Saving the test data from the User Maintenance window ......................................................................................169
System Overview 9
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Saving test data ................................................................................................................................................169
Saving reaction data in CSV format.......................................................................................................................170
Saving a data archive from User Maintenance window .........................................................................................171
Viewing data that was previously archived........................................................................................................172
8 System Setup .......................................................................................................................................174
Calibrating the touch screen monitor ................................................................................................... 174
Calibrating the touch screen..............................................................................................................................174
Configuring serum indices.....................................................................................................................174
Connecting to your host computer .......................................................................................................174
Cup/Tube Assignment ............................................................................................................................ 175
Setting up the ISE ...................................................................................................................................176
Activating the ISE..............................................................................................................................................176
Setting up ISE parameters ................................................................................................................................176
ISE calibration ...................................................................................................................................................176
Appendix A – Safety Information ..........................................................................................................178
Warning and hazard statements............................................................................................................ 178
Biohazard warning .................................................................................................................................................178
Electrical warning...................................................................................................................................................178
Laser warning ........................................................................................................................................................178
Household bleach warning.....................................................................................................................................179
Regulatory compliance ..........................................................................................................................................179
Laser information ..............................................................................................................................................179
Appendix B – Warranty and Support Information ...............................................................................183
Limited Instrument Warranty and Service Delivery Policy ................................................................. 183
Warranty period .....................................................................................................................................................183
Additional service period........................................................................................................................................183
Service calls...........................................................................................................................................................184
Service during normal hours .............................................................................................................................184
Extent of a service call ......................................................................................................................................184
Service outside normal hours............................................................................................................................184
Replacement of parts.............................................................................................................................................184
Warranty and service exclusions ...........................................................................................................................184
Design changes and retrofitting of instruments .................................................................................................186
OSHA requirements ..........................................................................................................................................186
Software License ...................................................................................................................................................186
ADVIA Contact information ....................................................................................................................................187
Siemens Worldwide...........................................................................................................................................187
Appendix C –Customer replaceable parts............................................................................................188
Appendix D – Specifications.................................................................................................................. 190
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Specifications – all .................................................................................................................................................190
Appendix E – Symbols ...........................................................................................................................194
Explanations of symbols associated with the ADVIA 2400 system ..................................................194
Warning and caution symbols................................................................................................................................194
System operation symbols.....................................................................................................................................195
System rating label symbols ..................................................................................................................................196
Hardware component symbols ..............................................................................................................................197
Ancillary reagent symbols......................................................................................................................................197
Connector symbols ................................................................................................................................................198
System packaging label symbols...........................................................................................................................198
System Overview 11
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1 System Overview
Figure 1-1. The ADVIA 2400 Chemistry System
The ADVIA® 2400 Chemistry System is an automated, clinical chemistry analyzer that
can run tests on human serum, plasma, or urine in random access and batch modes at a
throughput rate of 1800 photometric tests per hour and 600 electrolyte tests per hour.
The ADVIA 2400 Chemistry System is for in vitro diagnostic use.
Operating principle
This sequence summarizes a photometric analysis on the Chemistry System:
1. The first reagent (R1) for a test is aspirated from reagent tray 1 and dispensed by the
reagent probe into the cuvette in the reaction tray.
2. Samples on the sample tray or rack handler are aspirated and diluted by the dilution
probe, then dispensed into cuvettes in the dilution tray.
3. The dilution mixer stirs the diluted sample.
4. The sample probe dispenses the required amount of diluted sample into the RRV
cuvettes (the reagent is already in the cuvettes).
The system can use the remaining diluted sample in the DTT cuvettes for additional
tests on a workorder, a rerun, dilution, or reflex testing.
5. The reaction mixer 1 mixes the first reagent and the sample.
6. The second reagent (R2) for a test is aspirated from reagent tray 2 and dispensed by
the reagent probe into the cuvette in the reaction tray.
7. The reaction mixer 2 mixes sample and reagent 1 and reagent 2.
8. The reaction takes place for the amount of time designated in the assay.
9. The spectrophotometer obtains the concentration data every six seconds.
System Overview 13
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For each measurement, the RRV moves the cuvettes in front of the
spectrophotometer.
10. Cell blank measurements are performed at each wavelength.
11. The RRV cuvettes are washed when measurement is complete.
12. When the analysis is complete, the lamp energy is checked at each wavelength.
Hardware Overview
ISE location
1)
2) Pure water bottle
3) Horizontal pumps
4) Vertical pumps
5) ISE buffer bottle
Figure 1-2. Analyzer front view
6) Reaction bath oil bottle
7) Isotonic saline diluent bottle
8) Cuvette detergent bottle
9) Cell conditioner bottle
10) Reaction bath oil heater
11) Refrigerator filter
12) Reaction bath oil pump
13) Reaction bath oil filter
14) Power panel
15) Display panel
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Display and power panel
Figure 1-3. Power panel
1) READY lamp lights when the instrument is
ready.
2) START lamp lights when analysis is being
performed.
3) ALARM lamp lights when a problem occurs.
4) SYSTEM RESET button resets the computer
controlling the instrument (not normally used).
5) EMERGENCY STOP button is pressed to
stop the instrument in an emergency.
WARNING
If you stopped the system using the
Emergency Stop button, you must
perform a Weekly wash (WASH2)
prior to processing samples.
6) OPERATE/STANDBY switch turns the
analyzer power ON (OPERATE) or OFF
(STANDBY).
7) POWER lamp lights when the analyzer power
is ON.
Analyzer back view
Figure 1-4. Analyzer back view
1) Main Power Switch
(powers the entire
instrument)
2) Water Supply and
Drainage Panel
3) Circuit breaker panel
System Overview 15
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External Circuits
Maximum supply current of outlet = 7.5 A at 100 Vac
Connected Equipment
Rack handler, Workstation
Analyzer top view
1) Sample tray
2) Sample-dilution probe (DPP)
3) Dilution mixer (DMIX)
4) Dilution tray (DTT)
5) Dilution washer (DWUD)
6) Sample probe (SPP)
7) Reaction tray washer (WUD)
Figure 1-5. Analyzer top view
8) Reaction mixer 2 (MIXR2)
9) Reaction mixer 1 (MIXR1)
10) Reaction tray (RRV)
11) Reagent tray 2 (RTT2)
12) Reagent tray 1 (RTT1)
13) Reagent probe 1 (RPP1)
14) Reagent probe 2 (RPP2)
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Vertical Pumps
1)
Dilution wash pump (DCP)
6) Reagent dispensing pump 1 (RP1)
2) Dilution aspiration pump (DIP)
3) Dilution discharge pump (DOP)
4) Sampling wash pump (SCP)
5) Sampling pump (SP)
Figure 1-6. Vertical pumps
Horizontal Pumps
1) Dilution cuvette wash pump 1 (DWP1)
2) Dilution cuvette wash pump 1 (DWP2)
3) Dilution cuvette wash pump 1 (DWP3)
7) Reagent wash pump 1 (RWP1)
8) Reagent dispensing pump 2 (RP2)
9) Reagent wash pump 2 (RWP2)
7) Reaction cuvette wash pump 1 (WP1)
8) Reaction cuvette wash pump 2 (WP2)
9) Reaction cuvette wash pump 3 (WP3)
4) Dilution cuvette wash pump 1 (DWP4)
5) Switching valve (WCV2)
6) Switching valve (WCV1)
Figure 1-7. Horizontal pumps
System Overview 17
10) Reaction cuvette detergent pump 1 (DTP1)
11) Reaction cuvette detergent pump 2 (DTP2)
Page 18
Workstation (front view)
Figure 1-8. Workstation front view
PC (front view)
1) Mouse
2) Touch-screen monitor
3) Printer
4) Keyboard
5) Personal computer (PC)
Figure 1-9. PC front view
1)
Access door
2) CD-RW drive.
3) PC power switch. Used to turn ON or OFF the power
for the personal computer. Normally, it is left ON.
4) PC hard disk drive access lamp. Lights when reading
or writing to the PC hard disk.
5) PC power lamp. Lights when the power for the
personal computer is ON.
6) USB ports
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PC (back view)
Figure 1-10. PC back view
PC power connector
1)
2) Mouse connection
3) Keyboard connector
4) Communication port 1 (LIS-TDC)
5) Printer connector
6) LCD monitor connection
7) Analyzer Ethernet connection
8) Communication port 2 (URH)
9) Sleep ITF board potentiometer
Sampling and analysis
Sampling mechanisms
The sample probe (SPP) aspirates sample from the dilution tray (DTT) and dispenses it
into reaction tray (RRV) cuvettes for analysis, according to specified conditions. The
sampling pump (SP) handles the aspiration and dispensing functions.
Sampling operation
At initialization, the SPP moves (in the up position) to the RRV cuvette, then stops above
the wash port. The pumps stop when the SPP is positioned over the RRV cuvettes.
Each sampling cycle consists of these steps:
1. The SPP moves to the aspiration position of the DTT and aspirates the sample.
2. The SPP moves back to the wash port, where deionized water washes the SPP’s
outside surface.
3. The SPP moves to the RRV and dispenses the sample into a cuvette.
4. The SPP returns to the wash port, where degassed water washes the inside and
outside of the SPP.
The SPP is now ready for another cycle.
System Overview 19
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If an assay is not running, you can operate the unit manually from the Manual Operation
window.
Sample probe
1) Wash port
2) Sampling probe (SPP)
Figure 1- 11 Sample probe
After aspiration, the SPP is lowered into an RRV cuvette, where it dispenses the sample.
The probe’s tip is 2 mm deep into the solution, ensuring that no droplet remains on the tip
after withdrawal.
NOTE: Unlike the sample-dilution (DPP) and the reagent probes (RPP1 and RPP2), the
SPP does not have liquid level sensing. This is because the DTT cuvettes are always the
same size.
Sample pump
1) Sampling pump (SP)
2) Sampling wash pump (SCP)
Figure 1- 12 Sample pumps
The sampling pump (SP) withdraws sample from DTT, and also dispenses sample into
the RRV cuvettes. The SPP executes both actions. During both actions, the sampling
pump valves (SPEV1 and SPEV2) are closed.
After the sample is aspirated, the SPP moves to the wash port, where its outside is
washed. Sample probe valve (SPEV2) is open during this process, allowing water to flow
over the outside of the SPP.
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After the sample is dispensed, the SPP moves back to the wash port, where its inside is
washed. Sample probe valve (SPEV1) opens, allowing the SCP to send degassed water
through the SPP’s inside.
After the wash, the water drains down the wash port.
Clot detection
The clot detection system utilizes a pressure transducer to monitor the pressure in the
sample dilution probe line (DPP) for a complete obstruction during the sample aspiration
and dispensation cycle. Clot detection is available on the DPP line only.
The system monitors the pressure at the transducer at four points during the sampling
cycle and will display an error if a predefined limit is exceeded at any of the checkpoints.
A fifth check occurs at the start of sampling.
• Clot detection can be toggled on and off.
• Clot detection can be deactivated for calibrator and control materials that are run
• Clot detection is active for samples run from the STT, CTT, rack handler or other
The clot detector is active each time the DPP probe enters a sample, even when multiple
aspirations are required. If a sample is to be aspirated three times, the DPP will make all
three aspirations, even if a clot is detected. This may cause partial results to be reported.
When a sample is flagged for a clot, all of the ordered tests for the sample must be rerun.
from the STT or CTT.
LAS device. Clot detection is inactive when the DPP aspirates from the DTT.
Reaction tray
Figure 1- 13 Reaction tray
For each sample assay, the reagent probes (RPP1 and RPP2) dispense reagent into
cuvettes in the reaction tray (RRV). Then the sample probe (SPP) dispenses diluted
sample into the cuvettes. The mixture is then stirred by the reaction mixers (MIXR1 and
MIXR2), producing the desired reaction.
For analysis, the reaction tray rotates the cuvette in front of the spectrophotometer, where
the cuvette’s absorbance is measured. After analysis, the cuvettes are washed by the
reaction washer (WUD).
Reaction tray (RRV)
1)
2) RRV cuvettes (immersed in oil
heat bath)
System Overview 21
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The RRV contains 340 reusable cuvettes (20 sets of 17 cuvettes).
The RRV cuvettes are immersed in the reaction tank in an oil-heat bath. This keeps the
cuvettes at a constant temperature of 37°C for sample analysis.
Reaction tray mixers
Figure 1-14 Reaction tray mixers
The reaction tray (RRV) mixers (MIXR1 and MIXR2) stir the contents (sample and
reagent) of RRV cuvettes brought to their respective mixer positions.
1)
Mixer 1 (MIXR1)
2) Mixer 1 wash port
3) Mixer 2 (MIXR2)
4) Mixer 2 wash port
Mixing is performed using a spinning, reciprocating, and vibrating paddle. Strong and
weak stirring options are available.
Both mixers are located behind the reaction tray. MIXR1 mixes sample with reagent 1
(R1). MIXR2 mixes sample with reagent 2 (R2).
Reaction tray wash mechanisms
Reaction washer (WUD)
1)
22 ADVIA 2400 Operator’s Guide
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Figure 1-15 Reaction tray washer
The reaction washer (WUD) washes reaction tray (RRV) cuvettes after sample analysis is
complete. This allows the reuse of cuvettes without the risk of contaminating the next
sample.
The WUD has seven nozzles, each performing a stage of the wash. Each nozzle works on
a different cuvette, so the WUD washes seven cuvettes simultaneously (the cuvettes are
being washed in different stages at the same time).
After a cuvette is washed by one nozzle, it moves to the next until washing is complete.
While the RRV rotates, the WUD is in the up position.
The wash liquids pass through a preheater before they reach the WUD.
System Overview 23
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Reaction tray washer operation
When initialized, the WUD moves to the up position. If it is already up, it is lowered,
then raised.
To advance the cuvettes to the next wash nozzle, the RRV rotates a full-turn.
During each rotation (2-second cycle), the nozzles operate as follows:
Nozzle Probe Description
1 First nozzle A Aspirates reaction liquid.
B1 Dispenses wash water.
H1 Absorbs overflow liquid (abnormal
2 Second nozzle C1 Aspirates wash water.
D Dispenses detergent.
H2 Absorbs overflow liquid (abnormal
3 Third nozzle E Aspirates detergent.
B2 Dispenses wash water.
H3 Absorbs overflow liquid (abnormal
4 Fourth nozzle C2 Aspirates wash water.
F Dispenses cell conditioner.
H4 Absorbs overflow liquid (abnormal
5 Fifth nozzle G Aspirates cell conditioner.
B3 Dispenses wash water.
H5 Absorbs overflow liquid (abnormal
6 Sixth nozzle C3 Aspirates wash water.
7 Seventh
nozzle
D
conditions).
conditions).
conditions).
conditions).
NOTE: The fourth (4) and fifth (5) nozzles are
separated by a width of six cuvettes.
conditions).
Figure 1-16. Nozzle separation
24 ADVIA 2400 Operator’s Guide
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Reaction tank
Figure 1-17 Reaction tank
The reaction tank contains non reactive oil, which keeps the temperature of the liquid in
reaction tray (RRV) cuvettes at a constant 37 °C ±0.1 °C. The temperature is controlled
by a heater and a thermostat.
Sample tray
Reaction tank
1)
2) Three liquid surface level sensors
Sample tray
The sample tray holds patient samples, controls, calibrators, and diluents for
measurement. The tray rotates to move the samples to the aspiration position.
The sample tray has two sections:
STT (outer section): Used for general samples and reference samples for multipoint
calibrations. It has two rows, each with 42 positions (total 84). You can place serum or
urine samples into each position.
A barcode reader identifies samples in the STT. It can interpret barcode formats Code 39,
Interleaved 2 of 5, Codabar, and Code 128 format A, B and special characters ( . - + / * $
% ).
CTT (inner section): Used for calibrators, controls, and special purpose diluents. It has
two rows. The outer row has 34 positions and the inner row has 27 positions (total 61).
The CTT is water-cooled.
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1) Sample Barcode Reader
2) Sample Tray (STT)
3) Sample Tray (CTT)
Figure 1-18. Sample tray
Sample tray operation
At initialization, the tray rotates clockwise until STT position 1 is in the aspiration
position.
After you start a run, the sample tray moves clockwise to position 1, then it rotates
clockwise to move samples successively to the aspiration position.
If samples are identified by barcode, each sample detected by the barcode reader stops (in
turn) in the aspiration position. If samples are identified by position number, they move
by position number to the aspiration position. Regardless of the mode, you must enter
samples by a workorder, or they are not processed.
Monitor the progress of samples on the sample tray in the Test Result Monitor window.
If an assay is not running, you can operate the unit manually from the Manual Operation
window.
Container types
You place sample containers defined in the Order Entry window in the STT and CTT.
You can use 5-mL, 7-mL, or 10-mL collection tubes or 2-mL sample cups. You have to
put the small sample cups into plastic holders before you place them in the tray
positions..
The dead volume for the collection tubes is 200 µL, and the dead volume for the sample
cups is 50 µL.
You can use barcode labels on collection tubes but not on sample cups.
SMP Pause
Use the SMP Pause button at the Operation Panel to temporarily stop sampling so you
can add samples to or replace the STT/CTT tray.
NOTES
• The text on the SMP Pause button located on the Operation Panel is gray when
inactive and black when active.
• For more information on using the SMP Pause button, refer to the online
Operator’s Guide.
26 ADVIA 2400 Operator’s Guide
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Spectrophotometer
Figure 1-19. Location of the Spectrophotometer
The spectrophotometer measures the amount of light absorbed at 14 specific wavelengths
by liquids contained in reaction cuvettes.
Every six seconds, the reaction tray (RRV) moves cuvettes containing reaction liquid
(sample and reagent) in front of a halogen lamp, which sends light through the cuvettes.
Each time, a different wavelength is measured.
1) Photometer
2) Halogen Lamp
3) Cooling Tank
The photometer then measures the absorbance based on the lamp energy and the optical
density of the cuvettes. This process is repeated for as many times and wavelengths as
required by the assay conditions.
A cooling tank maintains the lamp temperature.
The system monitors the output energy of the halogen lamp during the cell blank check
and after each assay. The operator is alerted if the lamp performance is abnormal.
Use the Lamp Energy Monitor window to ensure that the halogen lamp is functioning
normally.
Reagents
Reagent mechanisms
The reagent probes (RPP1 and RPP2) aspirate reagent from the reagent trays (RTT1 and
RTT2) and dispense it into reaction tray (RRV) cuvettes for analysis, according to
specified conditions. Reagent pumps (RP1 and RP2) handle the aspiration and dispensing
functions.
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Reagent trays
Reagent trays 1 and 2 (RTT1 and RTT2) contain reagents used for assays (positions 1-46)
and the detergents used for daily washing and contamination prevention (positions 47-
50). The reagent probes (RPP1 and RPP2) aspirate the required reagent and dispense it
into the reaction tray (RRV) cuvettes for analysis.
Figure 1-20. Reagent trays
1)
Reagent tray 1 (RTT1)
2) Reagent tray 2 (RTT2)
Each tray has 50 positions. RTT1 contains the first reagent (R1); RTT2 contains the
second reagent (R2).
You can use any reagent container for more than one test item; a test item may require
more than one reagent container.
Each reagent tray has a barcode reader (RBC-1 and RBC-2).
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Reagent probes
Figure 1-21 Reagent probes
Reagent pumps
1)
Reagent probe 1 (RP1)
2) Reagent wash pump 1 (RWP1)
3) Reagent wash pump 2 (RWP2)
4) Reagent probe 2 (RP2)
1)
Reagent pump 1 (RP1)
2) Reagent pump 2 (RP2)
3) Reagent wash pump 1 (RWP1)
4) Reagent wash pump 2 (RWP2)
Figure 1-22 Reagent pumps
The reagent pumps (RP1 and RP2) withdraw reagent from the reagent trays, and also
discharge the reagent into the RRV cuvettes. The reagent probes execute both actions.
During both actions, the reagent pump valves (RPEV1-1, RPEV2-1, RPEV1-2, and
RPEV2-2) are closed.
After the reagent is aspirated, the probes move to the wash ports, where their outsides are
washed. RPEV2-1 and RPEV2-2 (wash cup valves for RPP1 and RPP2, respectively) are
open during this process. This allows water to flow over the outside of the probes.
After the reagent is dispensed, the probes move back to the wash ports, where their
insides are washed. RPEV1-1 and RPEV1-2 (valves for RTT1 and RTT2, respectively)
open, allowing the sample and reagent wash pump (SRWP) to send degassed water
through the probes’ insides.
After the wash, the water drains down the wash port.
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Reagent tray operation
At initialization, the trays rotate clockwise until reagent bottle 1 is in the aspiration
position.
After you start a run, the reagent trays move clockwise to position 1, then they rotate
clockwise or counterclockwise (whichever results in a smaller rotation) to move reagents
to the aspiration position. The number of trays and reagents used depends on the
specified assay conditions.
To check the reagent volume and number of tests remaining in a container, use the
Reagent Inventory window.
Reagent container types
Place reagent containers defined at the Reagent Container Settings window in the reagent
trays. The trays can hold 20-mL, 40-mL, or 70-mL wedge-shaped containers.
At this window, you also specify the reagent type (R1 or R2) for each reagent container.
Barcode labels on reagent containers have the test name, expiration date, lot number, and
container ID number. You can initiate a barcode scan at the Reagent Inventory window.
RGT Pause button
Use the RGT Pause button to temporarily stop testing, on the analyzer or from a lab
automation system, so that reagents can be added or removed at the reagent trays.
NOTES
• Do not press the RGT Pause button while a barcode scan is executing.
• The text on the RGT Pause button is gray when inactive and black when active. After
the button is selected, the RGT Pause process can take some time. The system will
safely complete any tests on samples that have been aspirated.
• For more information on using the RGT Pause button, refer to the online
Operator’s Guide under Reagent Management.
Sample aspiration and dilution mechanisms
The sample-dilution probe (DPP) aspirates sample from the sample tray (STT), from a
laboratory automation system (LAS), or from the universal rack handler, and dispenses it
into cuvettes in the dilution tray (DTT), according to the specified assay conditions. The
dilution pumps handle the aspiration and dispensing functions.
Using these mechanisms, you dispense the following items into the DTT cuvettes:
• sample diluted with standard diluent
• sample diluted with special purpose diluent
• undiluted sample
For more detailed information on how sampling is dispensed, refer to the online
Operator’s Guide under Hardware Overview – Sample dilution.
30 ADVIA 2400 Operator’s Guide
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Dilution tray mixer
The dilution tray (DTT) mixer (DMIX) stirs the contents of DTT cuvettes brought to the
mixer position. Mixing is performed using a reciprocating rod.
1) Dilution Mixer (DMIX)
Figure 1-23. Dilution tray mixer
Dilution tray mixer operation
At the time of system initialization, the mixer moves to the mixer position, then goes to
the down position. If it is already at the mixer position, it is raised, then lowered.
During each 2-second cycle on the DTT:
• The mixer moves up and over to the wash port, where it is washed with deionized
water. During this period, the dilution mixer wash valve (DMEV) is open, and
(briefly) the mixer rod is on.
• The mixer is raised and moved to the mixer position, where it is lowered into the
DTT cuvette. The rod is turned on to mix the sample.
• The mixer is raised from the cuvette and moved back to the wash port. The next
cycle begins.
If an assay is not running, you can operate the unit manually at the Manual Operation
window.
Dilution tray wash mechanisms
The dilution washer (DWUD) washes dilution tray (DTT) cuvettes after sample analysis
is complete, so they can be reused without risk of contaminating the next sample.
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1)
Dilution Washer (DWUD)
Figure 1-24. Dilution tray wash mechanism
The DWUD has ten nozzles, each performing a stage of the wash. Each nozzle works on
a different cuvette, so the DWUD washes ten cuvettes simultaneously. The cuvettes are
being washed in different stages at the same time.
After a cuvette is washed by one nozzle, it moves to the next until washing is complete.
While the DTT rotates, the DWUD is in the up position.
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Dilution tray wash mechanism operation
At initialization, the DWUD moves to the up position. If it is already up, it is lowered,
then raised.
During each 2-second cycle on the DTT, the 10 nozzles operate as follows:
Nozzle Probe Description
1
First nozzle
2 Second nozzle
3 Third nozzle
4 Fourth nozzle
5 Fifth nozzle
6 Sixth nozzle
7 Seventh nozzle
8 Eighth nozzle
9 Ninth nozzle
C1
A1
B1
C2
A2
B2
C3
A3
B3
C4
A4
A5
C5
Aspirates wash water.
Dispenses sample and wash water.
Absorbs overflow liquid (abnormal
conditions).
Aspirates wash water.
Dispenses sample and wash water.
Absorbs overflow liquid (abnormal
conditions).
Aspirates wash water.
Dispenses sample and wash water.
Absorbs overflow liquid (abnormal
conditions).
Aspirates wash water.
Dispenses sample and wash water.
Absorbs overflow liquid (abnormal
conditions).
Aspirates wash water.
10 Tenth nozzle
Figure 1-25. Nozzle separation
If an assay is not running, you can operate the unit manually at the Manual Operation
window.
System Overview 33
C6
D
Aspirates wash water.
Vacuums remaining liquid from cuvette.
Page 34
ISE (electrolyte analyzer)
The ISE measures the amount of sodium (Na), potassium (K), and chloride (Cl) in serum
or urine samples through voltage measurement by ion-selective electrodes.
The sample-dilution probe (DPP) aspirates the sample for electrolyte analysis. The
electrolyte analysis uses buffer as reagents.
In a two-stage process, the buffer voltage is measured, then the sample voltage is
measured. The difference between these voltages, the reference voltage, and the
temperatures of the liquids determine the concentration of Na, Cl, and K in the sample.
1) Reference Electrode
2) Na Electrode
3) K Electrode
4) Cl Electrode
5) Mixer
Figure 1-26. ISE components
To ensure data accuracy, undiluted sample is aspirated from the sample tray and
electrolyte analysis is always performed before photometric sampling.
34 ADVIA 2400 Operator’s Guide
6) Dilution Probe (DPP)
7) Sample
8) BPEV
9) ISE Degassing Unit
10) Buffer Solution
11) Buffer Pump (BP)
12) Temperature Stabilizer
13) DPEV
14) DP
15) Waste Block
Page 35
Universal rack handler – functional description (optional)
The universal rack handler is a separate sample delivery mechanism designed to allow
operators to continuously load samples. The operator can load five sample containers into
each rack and up to fifteen racks (75 samples) on each rack carrier to improve workflow
efficiency. The sample-dilution probe (DPP) aspirates the sample on the rack handler just
as it does from the sample tray, from an aspiration position specifically designed for the
universal rack handler or another laboratory automation system (LAS).
Use the universal rack handler for routine patient sample analysis only.
Process calibrators, controls, special diluents, and reruns with the onboard sample tray
(STT).
1)
Laser Station 1 (LS1)
2) Conveyor 1
3) Conveyor 2
4) Main PCB Chassis
5) Cross Drive
6) Conveyor 3
7) Sampling Station (LS2)
8) Outfeed Buffer
Figure 1-27. Universal rack handler components
System Overview 35
9) Outfeed Pusher
10) READY/STANDBY Switch
11) Infeed Pusher Arm
12) Infeed Tray
13) Display Panel
14) Rack-load Status Indicator
15) Outfeed Tray
Page 36
Software Overview
This section of the manual describes the software windows available to configure and run
your chemistry system. It does not explain how to use these windows. For detailed
information on the use of the windows, refer to the online Operator’s Guide.
The software controls most of the functions run by the chemistry system. The software,
which runs under the Windows operating system, starts automatically when you turn on
the PC.
You start the software from the Startup window, and stop it from the Menu Panel.
After the software has started, the Menu Panel and the Operation Panel appear at the top
of your screen.
As long as the software is running, these windows remain in the same position (unlike
most Windows XP windows). Use the top display option (Systems(s)) to allow other
windows to display on top of the Menu Panel and Operation Panel.
Startup window
The Startup window is the first window you see when you turn on the PC and the last
window when you stop the system.
Perform any of the following tasks at this window:
• new start
• restart
• back up and restore system files
• set or clear the system data prior to startup.
• shut down and restart Windows
1. At the Menu Panel, select
2. Select
If you select
able to perform the above functions). To open the Startup window again, restart
Windows.
Yes when prompted, then select Yes when prompted again.
The
Startup window appears.
Cancel, the Startup window closes, leaving you in Windows (you are not
System (s), then select Exit (X).
Operation Panel
This window opens after you start the system software from the Startup window.
Use this window for the following tasks:
• perform routine tasks on the Chemistry system
• check the system status and alarm messages
36 ADVIA 2400 Operator’s Guide
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Buttons
You select buttons on the Operation Panel to perform routine tasks.
Start
RGT
Wash Initialize
Pause
Stop
SMP
Pause
Figure 1-28. Operation Panel buttons
Prime Host
Error log Audible
On/Off
Alarm
Status and message boxes
The boxes on the right of the Operation Panel provide information on current system
conditions.
1)
Operating modes
3) Alarm message box
Operator's
Guide
2) Load status indicator
Figure 1-29 Status Message boxes
4) Time remaining indicator
Other tasks accessed from System(s) on the Operation Panel
Use the System(s) list to perform these tasks. To open the menu, select System(s) at the
top left of the panel.
System Overview 37
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Menu Panel
The Menu Panel opens after you start the system software (from the Startup window).
The window contains the buttons shown below. Use these buttons to access other menus.
Request
Calibration
Maint.
Reagent
Figure 1-30 Menu Panel
QC
Setup
Other tasks accessed from System(s) on the Menu Panel
1. To access the drop-down menu, select System(S) at the top left of the window.
2. Perform any of the following tasks:
• log on at a different access level
• change the system password
• display version information, and read or write a memo
• print the screen
• log on as a different user
• exit the system
Logging on at a different access level
Use this procedure to log on as a supervisor or tech_manager (when you start the system,
you are automatically logged in as a user). If you are already logged in as supervisor or
tech_manager, you can log back in as a user.
1. At the System(S) menu, select
Password.
2. Enter the new user name (access level) and the corresponding password.
Or, do not type a password at the Password window to log in as a user.
3. Select
OK.
You are now logged in as the new user.
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Changing the system password
Use this procedure to change the password for the supervisor ID.
1. At the System menu, select
Change password.
2. At the Change Password window, enter the old password, then enter the new
password.
3. Enter the new password again in the Confirm password box.
If you are setting the password for the first time, leave the Old password box blank.
4. To change the password, select
Change.
The next time someone logs in with this ID, the new password will be required.
Displaying version information and reading or writing a memo
Use this procedure to get information about the files currently in the system software.
1. At the System menu, select
Version info.
The Version Information window displays the system version number and other
information.
2. To close the window, select Return.
Printing the screen
1. At the System menu, select
Screen Print.
The entire screen (as it displays on your monitor) prints.
2. Print the screen to the clipboard.
Logging on as a different user
Use this procedure to log on a different user. All results obtained for this operator
annotates to the corresponding user code from the User Code Settings window.
1. At the System(S) menu, select
Change User.
2. In the Please enter password box, enter the user password.
User passwords assigned in the User Code Settings window are not case sensitive.
3. Select
OK.
You are now logged in as the new user.
Exiting the system
Use this procedure to close the system software.
1. At the System(S) menu, select
2. When prompted, select
Yes, then select Yes when prompted again.
Exit(x).
In a few seconds, the Startup window opens. From there, you can restart the software
or shut down Windows.
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Description of software windows
Menu items followed by 1 asterisk (*) are only available from the tech_manager logon.
Request windows
Select the Request button to display the following Request menu items
Order Entry window
Each patient sample must have a workorder that contains a sample number and a request
for at least one test.
Workorders can be created at your host computer or at the chemistry system.
Perform any of the following tasks at this window:
• use workorders created at your host computer
• create workorders at the analyzer
• create an individual workorder
• create multiple workorders (batch)
• change an individual workorder
• change multiple workorders (batch)
• create a load list
• create a profile
• instruct the system to use system-assigned position numbers
• configure the Order Entry window
Sample Log window
Perform any of the following tasks at this window:
• view the sample log entries
The following information is provided for each sample log entry:
Asp Date and Time External Type
STT Sample Id Rerun
CTT Description Results
• search the sample log
• delete a specific sample log entry
• delete all sample log entries
• print a list of the sample log entries
• export the sample log entries
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Test Result Monitor window
Use this window to monitor an analysis while it is running.
At the Test Result Monitor window, you can monitor the following:
• processing status
• The center of the window resembles the sample tray. The outer ring is the STT tray;
the inner ring is the CTT tray. Each tray position is a circle.
• As the run continues, the circles containing samples change color. The colors indicate
the current status of each sample. The color codes are displayed at the lower left of
the window.
NOTE: When running controls, please wait, there is a delay before the color coding
updates.
Sample test status color codes
The Test Result Monitor window uses seven color codes to represent sample status,
and the seven codes are displayed on the window. The Review/Edit window uses
these seven plus an eighth to indicate that a test for the sample was ordered but not
completed. Discrepancies in color are due to the windows’ displaying different
information. For example, a "complete" sample appears as dark blue on the Test
Result Monitor window, indicating that sampling is complete, while in the Review/
Edit window the same sample appears as pale blue, indicating that some tests are
missing.
No sample (white)
The position number does not contain a sample for this run
Untested (gray)
The run began and a workorder was created for this sample, but the sample was
not yet aspirated from the sample tray.
In process (pink)
At the Test Result Monitor window – the sample was aspirated from the sample
tray and was added to the dilution tray.
At the Review/Edit window – the sample is being analyzed or is being rerun.
Complete (blue)
At the Test Result Monitor window – the sample has been aspirated
At the Review/Edit window – analysis or the rerun for the sample is complete,
and the resultant data is produced.
Pending run (yellow)
The sample was analyzed but it must be reanalyzed. The reanalysis has not yet
started.
System Overview 41
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Being rerun (pink)
The sample is in the process of being reanalyzed.
Not complete (light blue)
The sample is in the process of being reanalyzed.
Rerun completed (green)
The requested rerun for this sample is complete and the resultant data is
produced.
Figure 1-31. Sample test status color codes
Sample information and system status
The button bar at the top of the window displays the Sample Search and Rack or LAS.
Sample Info. buttons.
Press the
Sample Search button to display a dialog box where you can search for sample
information by sample number by STT position (tray number and sample position), or by
rack/LAS position (rack number and sample position).
The search returns a Sample Information window showing the sample number, position
number, sample status, and the time remaining to complete the analysis of the sample.
If a rack handler or laboratory automation system (LAS) is in use, select the
LAS. Sample Info.
button to display the following sample information in the Rack or
Rack or
LAS. Sample Information window:
• Sample barcode number.
• Sample Status (see the status codes listed on the Test Result Monitor window).
• The time remaining to complete the processing of the sample.
NOTE: If processing has completed or if there are no samples available for processing
when you select the Rack or LAS. Sample Info. button, the system displays a message
that no sample information is available.
The left side of the Test Result Monitor window displays three panels.
The System Status panel shows the current operating mode of the system.
The Sample information panel shows the sample number and sample position of the
currently selected position on the STT/CTT graphic. If this is a barcode analysis, the
barcode number displays, and the position number is 0-00.
The code panel shows the color codes used in the STT/CTT graphic to represent sample
status.
In the middle of the sample tray display, the tray (TT) number for the current run displays
in the TT No. list box. If this is a barcode analysis, the TT number is 0.
To view the status of prior tray samples that are still in process, select the down arrow of
the TT No. list box, then select the number of the tray you want to view.
42 ADVIA 2400 Operator’s Guide
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Review/Edit window
CAUTION
Tests on the Review/Edit window must be in the same order as they appear in the
System Test List window to avoid incorrect positioning of results on the print report.
You must not modify the order of the test items on the System Test List window after
initial setup. If you reposition any test items on the System Test List window, results
for samples already run could be associate with the wrong test name.
Perform any of the following tasks at this window:
• review sample results
• configure reruns
• print selected patient results
• transmit selected patient results
Reaction Monitor window
Use this window to observe changes in reaction data. The changes are depicted as a
function of time, or of the 98 detection points in an analysis.
In the window, you choose a test and a sample for which you want to display a reaction
over time. Once you make your choice, a graph is created. You can choose several plots
for the graph.
Perform any of the following tasks at this window:
• Check test reaction data over time
• Show the reaction data
• Check the time courses for all wavelengths
• Check cell blank values
• Change the scale of absorbance graph
• Create a file containing test data
• Print the data list
RealTime Monitor window
The system monitors calibration, control, and patient sample results in real time. Only
patient and control sample results can be transmitted to a host computer. Results are
reported after the sample analysis is complete.
System Overview 43
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Print Report window*
Use this window to print sample data using report layouts created in the Print Format
Settings window.
This window is only available from the supervisor logon. To print reports, go to the
Review/Edit window and using the Print Report button.
CAUTION
Tests on the Print Report form must be in the same order as they appear in the System
Test List window, to avoid incorrect positioning of results on the print report. Do not
modify the order of the test names on the System Test List window after the initial
setup. Any repositioning of test names on the System Test List window could result
in already run being associated with the wrong test name.
Test Select window*
You select tests in this window for patient and control sample analysis and calibrations.
Use this window for the following tasks:
• Disable ("down") a test.
For example, a test may be downloaded from a host system to analyzers in your lab,
but that test is not run on the ADVIA
• To "down" a test, select one of the Test Select radio buttons, and deselect it from the
corresponding test table.
Chemistry System.
NOTE: You can remove tests for calibrators and controls from the current run using the
Calibration and Control windows, but they stay selected for subsequent runs.
Cup/Tube Assign window*
Use this window to assign container types to each of the 84 positions in the STT sample
tray. For the current analysis, you can override the settings in the Temp cup/tube select
window.
Test Utilization Statistics window
Use this window to monitor the test utilization against Patient, Calibrations, and Controls.
NOTE: All tests defined on the system are displayed.
44 ADVIA 2400 Operator’s Guide
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Correlation window*
Use this window to create and display correlation charts and the data that displays in the
charts.
When needed, use the correlation data to create a real time correction formula in the
Analytical Parameters (Chemistry) window (or ISE Parameters of Setting window).
Perform any of the following tasks at this window:
• display charts
• display chart data
• create charts (add data automatically)
Use this procedure if the measurement data comes from the system
• create charts (add data manually)
Use this procedure if the measurement data comes from another blood chemistry system
• print charts
• delete charts
Calibration windows
Select Calibration to display the following Calibration menu.
View Calibration Curve window
Use the View Calibration Curve window to review the detailed calibration curve data for
a method, to restore a calibration, to monitor calibration trends, to obtain a summary of
all calibration information and to view RBL and calibration check information.
NOTE: While running calibrations or processing samples, the View Calibration Curve
window is closed and cannot be viewed until the analyzer returns to the Ready or Wait
mode.
Calibration/RBL History
The system stores data for up to 100 calibration/RBL events for each of 2
different reagent lot pairs (R1 and R2), for a total of 200 data sets per method.
Use the Calibration/RBL History window to:
• review the calibration history for all methods run on the system
• print the displayed calibration data
• generate a CSV file for displayed calibration data
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Sample Select window*
Use this window to select the samples for control sample analysis and calibrations, and to
temporarily disable a sample so that the rest of a run can proceed.
For example, if you only want to run ISE calibration on serum samples, disable the ISE
calibration urine samples. The run proceeds normally, but it does not include urine
samples.
Perform any of the following tasks at this window:
• select samples available for control analysis calibration
• print the settings
• clear the settings
Calibration Setup window*
Use the Calibration Setup window to enter the information required to calibrate each
photometric test.
You can request automatic recalibration for each test whenever a new reagent container is
loaded or after a user-specified time interval. When a time interval expires, the system
recalibrates at the start of the next run.
NOTE: You can run tests with 2 different lots of the same reagent but you cannot
calibrate the 2 lots on the system at the same time. You have to calibrate them one at a
time. After the system calibrates the lots separately, you can place both reagents back on
the system and run tests.
Perform any of the following tasks at this window:
• enter absolute or single-point (STD) calibration methods
• enter multi-standard calibration methods
• print the calibration setup information
• delete the calibration setup information
• configure the automatic calibration feature
Maintenance windows
Select Maint. to display the following Maintenance menu.
System Startup/Shutdown Setting window
Perform any of the following tasks at this window:
• perform a system-assisted startup (Start set)
• perform a system-assisted shutdown (Shutdown set)
• perform an automatic startup (Auto start set)
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User Maintenance window
Perform any of the following tasks at this window:
• water blank measurement
• cell blank measurement
• batch printing
• filing of measurement data
• save of text file
System Monitor window
Perform any of the following tasks at this window:
• verify operating conditions (daily procedures)
• set the system monitor (system settings)
System Maintenance Monitor window
Perform any of the following tasks at this window:
• enter schedules for maintenance tasks
• monitor the maintenance status of the system
• delete maintenance records
Lamp Energy Monitor window
Perform any of the following tasks at this window:
• check the spectrophotometer lamp energy
• perform offset analog to digital (AD) measurement
ISE Operation window
Use this window to perform various ISE tasks.
There is a display area at the top of the window where you can view the running status.
Perform any of the following tasks at this window:
• buffer prime
• calibration
• wash electrode
• CV check
• interval check
• selectivity check
• final operation
• manual operation
• ISE line wash
• Dilution bowl drain
• initialize
• batch print
• periodic wash
• enter information about each electrode
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ISE Monitor window
Perform any of the following tasks at this window:
• monitor and verify calibration data as a function of time, using history graphs
• monitor serum or urine calibrations
• monitor calibration trace data
• display data from past calibrations
• *monitor selectivity check data
• print the results
• delete calibration trace data
• change y-axis scale
• transfer calibration data to the ISE
* The selectivity check must be performed under the supervision of authorized Siemens
service personnel. Please call your local technical support provider or distributor.
Manual Operation window
Perform any of the following tasks at this window:
• operating a unit
• activating or deactivate a unit
• checking unit position values
BIOHAZARD
Wear personal protective equipment. Use universal precautions.
Manual operation of units may be necessary when performing maintenance and
troubleshooting tasks.
The window contains a graphical image of the analyzer which depicts the operation you
are performing.
IMPORTANT
The operating mode must be READY or WAIT for you to run manual operations.
Also, if you cannot perform an operation due to system restrictions, a buzzer goes off and
a message displays at the Alarm message box (in the Operation Panel). Select
ALARM in
the Operation Panel for more information and instructions regarding the buzzer.
On-Line Monitor window
Use this window to monitor the exchange of data between the system and the host
computer. The monitor displays the data being exchanged and the control signal codes
that ensure accurate transmission of information.
You can view up to 300 data items in the window.
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Error Report window*
Use the Error Report window to review system status and error messages. If the column
has a drop-down arrow you can sort the column. You can also search by Samp ID, Safe
No, and Test Name.
For each message, the following information displays:
Icon Samp.ID Safe. No.
No. Test Name Contents
Date Time Measures
Section FNO
Mode INDEX
NOTE: While the Error Report window is displayed, it is not updated. To view new
messages, you must close the window, and then reopen it.
Reagent windows
Select the Reagent button to display the following Reagent menu:
Reagent Inventory window
Use this window to monitor the reagents in the reagent trays (RTT1 and RTT2). The
Reagent Inventory window displays the status of all reagents loaded on the reagent trays.
The window continuously refreshes with new information, and it updates after each
barcode scan.
The Reagent Inventory window automatically opens at start up and remains opened.
When using other windows or when you select the X in the upper right corner, the
window minimizes to the taskbar at the bottom.
The reagents listed in the window are in alphabetical order.
Perform any of the following tasks at this window:
• monitor reagent positions, number of tests, volumes, and days remaining.
• execute a barcode scan
• deselect a reagent
• print a reagent summary report
• view the Total Test summary
• view the calibration interval review
• pre-calibrate a new reagent lot
CTT Monitor window
Use this window to monitor the amount of liquid in containers on the CTT tray.
Reagent Container Settings window
Use this window to enter and display information for non-barcoded reagent containers
and start their Days Remaining counter. This window is updated automatically for
barcoded reagents.
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This information is used by the Reagent Inventory window.
Perform any of the following tasks at this window:
• set the reagent container type, lot number, and expiration date
• start the Days Remaining counter for non-barcoded reagent containers
• print the settings
• clear the settings
Active Test List window*
Use the Active Test List window to temporarily deactivate a method, so that methodrelated alarm messages (for example, missing reagents) are not generated. You would use
this function if, for example, for tests that you do not run every day.
After you make your selections, activating or deactivating a method, you must initialize
the system for the changes to take effect.
The Active Test List window displays all tests loaded on the system, but the deactivated
or unchecked tests are grayed out.
NOTE: At the Active Test List window, you must select all the tests you expect to run. If
a test is not selected, the system skips that test. An error message does not display in the
Alarm message box on the Operation Panel, but is recorded in the error message log.
Active is checked if the test is active.
Test Name displays the name and number of the test.
R1 Pos No displays the position of R1 on the reagent tray.
R2 Pos No displays the position of R2 on the reagent tray.
Reagent Information window*
Use this window to review and edit reagent information, such as on-system stability,
calibration frequency, and fill volumes for barcoded reagents and to enter information for
non-barcoded reagents.
The Reagent Inventory and Reagent Container Set windows use this information.
Assay Name is the test method name.
R-Code is the reagent code for each Siemens barcoded reagent container or a 5 digit user
defined code for non-barcoded reagents.
Days Remaining specifies the on-system reagent stability.
Comment is a user entered remark or reminder.
R1 and R2 Fill Volume are the fill volume in mL for reagent containers R1 and R2.
NOTE: For user-defined methods, the Assay Name, R-code, Days Remaining and R1-
R2 Fill Volume must be entered correctly. R2e is not used.
After system installation, all default information for Siemens-defined methods appears in
this window.
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Reagent Barcode Maintenance window*
NOTE: Access to this window is through a tech_manager and service logon level only.
Please contact your technical service provider or distributor in order to change the
default settings on this window.
Use this window to maintain barcode information for the reagents in RTT1 and RTT2. To
confirm that the correct barcode is scanned, use the Reagent Barcode Confirmation
window.
In this window, you can delete specific reagent codes and specific reagent barcodes from
the ADVIA reagent barcode database. Only use this function to delete an old reagent
code or reagent barcode that may have the same lot number and container number as a
new reagent container you want to place on the system. In most circumstances, you do
not have to use this functionality.
The barcode maintenance database can store a maximum of 200 reagent codes (assays)
and up to 2000 individual reagent barcodes. If the database exceeds the limit of 2000
barcodes, the oldest stored barcode is replaced with the most current barcode.
The reagents are listed in the same order as they display in the System Test List window.
The Reagent Barcode and Date Opened on System information for one or more reagents
displays when a Reagent Code is selected.
QC windows
Select the QC button to display the following QC menu.
ADVIA QC window
ADVIA QC provides both real-time and long term evaluation of analyzer and method
performance.
To automatically transfer data to this QC manager, at the System Monitor window, in the
ADVIA QC Transfer area, select Yes.
Perform any of the following tasks at this window:
• collect control results
• calculate and display statistical data
• assess data errors
• identify QC violations
• review control results
• create printed reports
• identify and report events such as lot changes and calibration dates
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Real-time QC window
Use this window to review the performance for two controls simultaneously using a
Levey-Jennings chart (x-chart) or a twin chart.
NOTE: This window is intended to be used when two controls are run for each test.
However, you can still use this window if you are running only one control.
Daily Precision Control window
Use this window to view daily control results.
CAUTION
You must not modify the order of the tests on the System Test List window after
initial setup. To avoid incorrect positioning of results on the print report, tests on the
Daily QC window must be in the same order as they appear in the System Test List
and Process Sequence windows. If you reposition any tests on the System Test List
window, results for samples already run could be associated with the wrong test
name.
NOTE: The maximum number of results displayed in the Daily Precision Control window
is 200. When this number is reached, the system deletes the results starting from the first
entry and adds new results after the latest entry. The system does not display a message
that the maximum number of results was reached.
Perform any of the following tasks at this window:
• view the Levey-Jennings charts (x-charts)
• view the detailed control data information and omit invalid control results
• view the QC summary list
• view the daily QC list
• use daily statistics to establish the Daily QC mean and 1 SD (standard deviation)
values
• print the daily x-charts
• delete the daily QC data
• update the QC Cumulative window
The daily mean and fluctuation range (R) for every test in each selected control are saved
in the QC cumulative window as a data point for the current day.
IMPORTANT
To avoid losing QC statistics, please observe the following:
Update the QC Cumulative window before more than 20 daily control samples are run.
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The QC Daily Precision Control window can manage a maximum of 20 results for each
control product (level). Only the most current QC data are saved when more than twenty
controls are run in a day. (If an additional control sample is run, the oldest control sample
is deleted to make room for the new one. Similarly, if multiple repetitions (up to 5) of a
control are requested when 20 results have already been stored, the system aspirates the
sample multiple times and saves the results as the last ones in the sequence, discarding
the same number at the beginning of the sequence.)
To avoid losing control results, you must perform a QC cumulative save on the QC Daily
Precision Control window at the end of each day, and perform a New Start when the
system is turned on the next day.
Do not update the QC Cumulative window more than once each day.
If you return to the QC Daily Precision Control window after performing a "QC
cumulative save," you must not perform another update.
You must not perform an update when there are no daily control data available. The
cumulative data point will be deleted for today and no additional control data can be
saved for the day.
QC Cumulative window
Use this window to manage the day-to-day (cumulative) QC information.
Perform any of the following tasks at this window:
• specify the time interval you want to review
• view the Levey-Jennings charts (x-r charts)
• view the detailed control data information and omit invalid control results
• view the QC summary list
• use cumulative statistics to establish the Daily QC mean and 1 SD values
• print the cumulative x-r charts
• delete the cumulative QC data
Control Data Setup window*
Use the Control Data Setup window to manually enter the control mean and 1SD limits
for evaluation of the daily and the cumulative control statistics. Typically, you enter
values obtained from the package insert of a commercial control.
You can also obtain the control mean and 1SD values from the actual control results
using the Daily Precision Control and QC Cumulative windows, and then allow the
system to load them automatically into the Control Data Setup window. For example: use
the procedures to enter data for a pool of patient samples you want to run as a control.
Perform any of the following tasks at this window:
• enter the mean and 1SD values
• print control data registration information
• delete control data registration information
• copy control data registration information
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Sample Select window*
Use this window to select the samples for control sample analysis and calibrations, and to
temporarily disable a sample so that the rest of a run can proceed.
For example, if you only want to run ISE calibration on serum samples, disable the ISE
calibration urine samples. The run proceeds normally, but it does not include urine
samples.
Perform any of the following tasks at this window:
• select samples available for control analysis calibration
• print the settings
• clear the settings
QC Sample Definition window*
Use the QC Sample Definition window for the following tasks:
• entering the information required to run samples of up to 26 control products
• requesting an automatic run of control(s) after a user-specified number of samples are
processed
Perform any of the following tasks at this window:
• enter the sample information for a control
• configure the automatic control feature
• print the control sample information
Setup windows
NOTE: You must log on as a supervisor, tech_manager, or service to access window
under the Setup button.
System Specification Settings window*
Use this window to enter settings related to the system configuration.
You can enter or change settings for the areas listed below:
• Settings that take effect after the next New Start
• basic system composition
• basic system operation
Settings that take effect after startup operation from READY state
• sample containers
• reagent bottles
• Serum indices specifications
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The settings below are related to serum information items (serum indices), which are
involved in creating workorders. If the serum indices for each sample are not selected in
the Order Entry window, select one of these settings:
• Request. item range
• Compulsory item analysis
• Not handled
• Set the system monitor
• Set system parameters
• Basic system composition
These settings allow you to customize general components of the system:
♦ ISE ♦ Sample delivery
♦ Sample bar code ♦ On-line
♦ Concentrated waste ♦ Reagent bar code
• Basic system operation
• These settings are used for retesting of samples
• Auto. retest
• Manual retest
• Sample containers
The settings below specify the sample containers used by the system. You can specify up
to 9 container types.
CAUTION
Normal operation and the volume of sample aspirated depend on the specifications
you enter here. Incorrect settings may damage the probes and nozzles.
♦ Type ♦ Container name
♦ Container diameters ♦ Container heights
♦ LLS sensitivity ♦ Liq volume judge
• Reagent bottles
The settings below specify the reagent bottles used by the system. These settings are used
in the Reagent Inventory, Reagent Container Set, and Lamp Energy Monitor windows.
CAUTION
Incorrect settings may result in abnormal data.
♦ Type ♦ Container name
♦ Bottle section ♦ LLS sensitivity
Analytical Parameters (Chemistry) window*
Use this window to set up chemistry methods.
Siemens methods are predefined when the system is received. For predefined methods,
only expected values, rerun conditions, and correction formulas should be modified.
You can define new methods. The system can store up to 200 chemistry methods in this
window (including predefined methods).
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Methods 1–150 are predefined methods. The analytical conditions are predefined. Some
of the fields of the analytical conditions are grayed out and cannot be edited.
NOTE: These are Siemens defined methods, only edit user-defined parameters.
Methods 151–200 are reserved for user-defined methods.
NOTE: When you change and save your settings in the analytical parameter fields the
version number in the upper-right corner of the window moves up one numerical
increment and the date is updated to the current date, for example, from Ver.
2.0.0.yyyymmdd to Ver. 2.0.1.yyyymmdd (in this example the 2 is the software version
and the first 0 is the method parameter revision minor number).
System Test List window
Perform any of the following tasks at this window:
• Add tests that are used regularly by your system. The tests on the list appear on other
windows
• Assign a reagent tray position number for non-barcoded reagents or a reagent code
(R-Code) for barcoded reagents
CAUTION
To avoid incorrect reporting of results at the Review and Edit, the Daily QC
windows, and the Print Report form, you
items on the System Test List window after initial setup. If you reposition any test
items on the System Test List window, results for samples already run could be
associated with the wrong test name.
must not modify the order of the test
Perform any of the following tasks at this window:
• add tests to list
• remove tests from list
• set the test processing order
Analysis order oet window
Use this window to set the order in which tests on the list run. To run tests in the order
they displays at the System Test List window, you do not need to use this window.
• print test list items
• assign reagent codes or position numbers
Analytical Parameters (Serum) window*
Use this window to set the parameters for evaluating the serum indices (lipemia,
hemolysis, and icterus).
Perform any of the following tasks at this window:
• define up to five different flagging levels of concern for each serum indices
• select a test item to perform the serum indices analyses
• enter the factor values required to calculate the serum indices result values
NOTE: Operation of the serum indices feature is controlled in the Serum set area of the
System Specifications Set window.
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Process Sequence window*
CAUTION
To avoid having quality control data reported for the wrong tests, do not switch
Process Sequence numbers
after control results have been accumulated. Patient
results are also affected.
Use this window to set the order in which:
• tests display at windows such as Order Entry, Test Select, and Real-Time QC
• tests print
Only tests set up in the windows listed below can be arranged at the Process Sequence
window:
• System Test List
• Analytical Parameters (Serum)
• ISE Parameter Settings
• Ratio Parameters
Ratio Parameters window*
Use this window to create up to 20 ratios with two test items in each. The ratio can
contain 2 tests.
Contamination Settings window*
Use this window to set the conditions for preventing contamination of reagent probes and
reaction tray cuvettes.
Each condition specifies an action to be taken if a specific interferer contaminates a
specific receptor. You can set up to 100 conditions each for reagent probes and RRV
cuvettes.
Perform any of the following tasks at this window:
• set conditions for avoiding reagent probe contamination
• set conditions for avoiding RRV cuvette contamination
• set conditions for anti-contamination detergent
• print the conditions
• clear the conditions
Ctrl/Cal Sample Setup window*
Perform any of the following tasks at this window:
• get or view measurement times, container types and comments for control and
calibration samples
The settings are available in the Calibration Setup, QC Sample Definition, and ISE
Parameter Settings windows for entering sample tray information.
• determine the tests set to run on a given position.
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User Code Settings window*
Use this window to enter a user code, user name, and user password for up to 50
operators who are authorized to use the chemistry system.
Perform any of the following tasks at this window:
• create/change entries for an authorized user
• delete the user code entries for a specific user
• delete all user code entries
• print a list of the user code entries
New Test Definition window*
Use this window to set up new tests, calibration definitions, sample container definitions,
and QC sample definitions. The New Test Definition window provides easy step-by-step
procedures.
ISE Parameter Settings window*
Use this window to set the analysis parameters for ISE measurement of Na, Cl, and K
electrolytes.
Perform any of the following tasks at this window:
• enter settings for individual electrolytes (Na, Cl, and K)
• enter settings for all electrolytes
• check CTT settings
Print Form Settings window*
Use this window to design or edit a print form for sample results. When you print reports
from the Print Report or the Review/Edit window, you will select a print form created
here.
There are two sample report formats provided:
• A Patient Chart report (Chart.frm) is formatted for a single sample.
• A Consolidated report (Consolid.frm) is formatted for multiple samples.
You create or edit each print form by placing design elements, such as free-text, sample
attributes, and result data (for example, test name, result value, and flags) on a form page
that has the same dimensions and orientation as the printed page. A grid overlays the
form page. This helps you to position precisely the design elements.
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Perform any of the following tasks at this window:
• start a new print form or open an existing one
• create or edit a print form
a. Select the page size, paper source, orientation, and margins
b. Determine the number of reports you want printed on each page
c. Select the default font
d. Set up the page grid
e. Define the "temporary text" that appears on form page in place of the actual data
f. Select the test items to be printed
g. Create the fixed character strings for use as free-text and labels
h. Select the sample attributes to be printed
i. Create the item cells to contain the results for each test item
j. Select the results data to be printed for each test item
k. Create the item comments (units), select packed or unpacked reports, and specify
different flag letters
l. Save the print form
• view the print preview
• print the print form
Online Settings window*
Use this window to setup the communications between the system and a host computer.
NOTE: Refer to the publication titled LIS Interface Guide for detailed information about
this communications protocol.
Perform any of the following tasks at this window:
• set the communication parameters that control the exchange of messages between the
system and your host computer
• test the serial port
• select the types of sample results and workorders you want transmitted
• select each test to be transmitted, and assign a host computer test number
• set up the Data Clean check to automatically validate results before transmission
Alarm Buzzer Settings window*
Use this window to determine when and how the alarm buzzer sounds to alert the
operator to status changes and system errors.
• set an alarm buzzer for a system error
• set an alarm buzzer for a status change
• print alarm buzzer settings
• delete alarm buzzer settings
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NOTE: Please contact your local technical support provider or distributor before
changing the default settings on this window.
System Parameters Settings window*
Use this window to enter or change system parameters, which define the system
processing environment.
Perform any of the following tasks at this window:
• define and edit system parameters
• view system parameters
• print system parameters
Reflex Test Settings window*
Use this window to define reflex testing for reagent methods. You can define up to three
reflex tests for any given test method based on a set of user defined conditions. When all
conditions are true, the specified reflex tests are added to a sample workorder. The
system tracks the workorder as a pending rerun until the reflex tests are completed.
Reflex tests display in the Prev. val. column in the Review/Edit window.
To define reflex testing for a given method, you must list the reagent in the System Test
List window.
Other Tasks
Use the System(S) menu in the top left corner to perform the following tasks:
• change the Top display option
• log on at a different access level
Use this procedure to log on as a tech_manager or a supervisor (when you start the
system, you are automatically logged on as a user). If you are already logged on as a
tech_manger or supervisor, you can log back on as a user.
• change the system password
Use this procedure to change the password for the supervisor ID.
• display version information and read or write a memo (see Version Information
Use this procedure to get information about the files currently in the software.
• print the screen
• log on a different user
Use this procedure to log on a different user. All results obtained for this operator will be
annotated with the corresponding user code from the User Code Settings window.
• exit the system
Use this procedure to close the software.
window below)
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Version Information window
Use this window to display version information for files listed below and currently in the
software. Select one of the options in the lower left corner of the window.
ADVIA o.p.
This option displays information on the program files in the system.
Column
Group Indicates the type of program. Programs related to the overall
Process Brief description of the program’s function (often the name of
Version The version of the program being used in this version of the
File name The name of the program file. A .EXE extension indicates the
File date/time The date the program file was created or last modified.
Size The size (in bytes) of the program file.
Description
system are in the System group. Other programs are part of
groups corresponding to their Menu Panel buttons (for
example, Request, Calib.).
the window it opens).
software (does not have to match).
file is a program.
Controller
This option displays information on the data tables in the system.
Column Description
File name The name and path of the table. A .TBL extension indicates
the file is a table.
Version The version of the program that is being used in this version
of the software (does not have to match).
Explanation Brief description of the file.
Safety Message
This option displays information on safety message files in the system.
Column Description
File name The name of the file. A .MSG extension indicates the file is a
message.
File date/time The date the file was created or last modified.
Size The size (in bytes) of the file.
Explanation Brief description of the file’s function.
Version
information
The version of the file being used in this version of the
software (does not have to match).
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Etc.
This option displays information on other files in the system, generally text files shown
for informational purposes.
Column Description
File name The name and path of the file. A .TXT extension indicates the
file is a text file.
File date/time The date the file was created or last modified.
Size The size (in bytes) of the file.
Explanation Brief description of the file’s function.
Version
information
The version of the file being used in this version of the
software (does not have to match).
Memo
Use this window to write or display memos about the system.
At the Memo window, you can do the following:
• read a memo
• write or edit a memo
• print a memo
• clear a memo
Using online help
The online Operator's Guide viewer has three functional areas: top (navigation toolbar),
left (navigation frame), and right (content frame).
Use the navigation toolbar to access the table of contents, index, search, glossary, and
print functions. The following are descriptions of the different areas of the online help.
1. Select
2. Select a given subject and a cascading list of relevant subtopics open. Then select a
3. Select
4. Enter the first few letters of the term you want to search for in the input box. The
5. Select the
6. The search interface is displayed. Enter the word you want to search for in the input
Contents tab and a table of contents displays.
subtopic to display it in the content frame.
Index and a searchable keyword index displays.
index scrolls automatically to the first occurrence of the input text string. Associated
help topics for the selected keyword are displayed in the bottom frame. Select a topic
name to display it in the content frame.
box and select the
occurrence of the word. This may take a few seconds. Help topics which contain an
instance of the word are displayed in the bottom frame. Select a topic name to display
it in the content frame.
Search tab.
button. The function searches the entire help database for any
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7. Select Glossary. A glossary of relevant terms displays. Select any word in the list to
display its definition in the bottom frame.
8. Select the
Print tab to print information displayed in the content frame. To print the
contents of a popup, right-select in the popup and select Print from the menu. If a
large image is partially displayed in the content frame, you must enlarge the viewer
to display the full image before it can be printed properly.
Viewing the content
Help topics display in a content frame. Some topics, including reference documents and
images, appear in a second window. Navigation from these windows, including
minimizing, maximizing and closing, use standard Windows conventions.
Some popups display partially outside the viewer content frame. In this case, to view the
entire popup, select and drag the lower-right corner of the viewer window to enlarge it
and view the complete popup.
Topics have hyperlinks (displayed in teal) or shaded hotspots embedded in text or
illustrations that link to displays of related or more detailed information.
Select teal text to activate hyperlinks.
The hyperlink is bold when the mouse pointer is over it. Hyperlinks using a cascading
menu point to additional links. Graphics may have hotspots (shaded green or tabs) that
are more detail information, such as a table of values.
1. Select the back arrow
on the toolbar to return to the previously viewed topic or
the forward arrow
to navigate to the topic that was displayed prior to going back.
2. To make a popup go away, select outside the popup window.
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default size.
System Overview 63
Page 64
64 ADVIA 2400 Operator’s Guide
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2 Operating the System
Start the System
Logging on
There are 4 access levels to the system software:
Level Password Comment
user no Intended for routine operation
supervisor yes Access to some system setup and advanced
maintenance features
tech_manager yes Access to most system setup and advanced
maintenance features
You have to contact your technical service provider
or distributor in order to change the default settings
on these windows.
service yes Access for Siemens service personnel
You are automatically logged on at the user level during startup. No operator is required.
If you log on at the supervisor or tech_manager levels, when done, you should log on to
the user level (no password required).
Starting the system
1. After the power is applied and the Windows operating system is loaded, the ADVIA
Chemistry system Startup window displays.
2. Turn on the optional universal rack handler:
a. For the rack handler, set the control panel Standby/On switch to | (ON).
The ready indicator is green.
b. For the universal rack handler, set the display panel Ready/Standby switch to
READY.
The power indicator is green.
3. At the analyzer power panel, set the Operate/Standby switch (
1) to Operate.
Operating the System 65
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Figure 2-1. Power panel at Startup
The power indicator (2) is on, and the Start (4) and Ready (3) indicators start flashing
when the communication to the PC is established.
4. At the Startup window, do the following:
a. In the Please enter password box, enter the user password.
User passwords assigned in the User Code Settings window are not case
sensitive.
b. Select New Start or Re-start, then select
OK.
After a few minutes, the Menu Panel and the Operation Panel open.
CAUTION
Make sure that all probes and mixers are free to move without obstruction and that all
analyzer covers are in place to avoid possible injury and damage to the analyzer
5. When the Start and the Ready indicators are off, and the Initialize button on the
Operation Panel activates (turns black), select
Initialize.
6. At the Windows desktop, double-select the rack handler icon.
7. Log on as
supervisor or tech_manager, if required.
Performing a system-assisted startup (Start set)
1. Verify that sufficient system reagents and wash solutions are available for the startup
you want to run.
2. At the Menu Panel, select
3. In the Mode set list, select
66 ADVIA 2400 Operator’s Guide
Maint. then select System Startup/Shutdown Settings.
Start set.
Page 67
4. Verify the selections for the startup you want to run.
If no startup is defined or you want to change an existing one, proceed as follows:
a. For each of the Proc. set, Set1, Set2, or Set3, you can select one of the following:
(1) In the PRIME list, select the prime you want run or select
NONE.
A Prime is required only if you replenish a system reagent or replace a
related component.
(2) Select the check box next to each wash you want to perform.
(3) Select
WASH3 for the startup wash.
(4) If you want to run Cell blank, select the check box next to Cell blank.
Run Cell blank weekly.
b. Select
c. To confirm, select
5. In the Select wash routine area, select
Save.
Yes.
Startup.
6. In the Proc.set list, select the startup setting (Set1, Set2, or Set3) you want to run.
7. Select
8. To confirm, select
Start.
Yes.
The current operating mode displays in the System mode box, and the time remaining
to complete the startup displays in the Time until end box. If a cell blank is run, the
system prompts you to save the results. When done, the system enters the Ready
mode.
9. To halt the startup, do the following:
a. At the System Startup/Shutdown Settings window, select
b. In the Select wash routine area, select
c. At the Operation Panel, select
Checking the analyzer
1. Inspect the following components:
NOTE: If any of the components require maintenance, refer to the Maintenance
Section of this guide or to the online Help for step-by-step procedures.
a. probes
b. mixing rods
c. dilution-cuvette washers (DWUD)
d. reaction-cuvette washers (WUD)
e. probe wash cups
f. cuvette covers
g. pumps for leaks
Stop.
Cancel.
Stop.
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2. Verifying system operating conditions.
a. At the Menu Panel, select
Maint., then select System Monitor.
Normal and abnormal indicators are listed below.
b. If there are abnormal indicators, take the appropriate corrective action.
NOTE: If you see an abnormal condition, check the Error Report window for more
information.
Operating condition Normal
RRV Bath Temp.
Temperature status of the heating bath fluid
in the reaction tray. The normal temperature
range is 37.0 ± 0.5°C. A temperature
exceeding 50°C with the thermostat on is
overheated.
RRV Bath Control
Circuit control that keeps the reaction
(RRV) bath temperature in range.
RRV Bath Circ.
Flow rate status of the heating bath fluid.
The status is determined by the flow rate in
the RRV Bath Circ. field.
RRV Bath Circ. Rate
Flow rate of the heating bath fluid, in
mL/minute. A flow rate <2000 mL/minute
or >6000 mL/minute is considered
abnormal.
RRV Oil Level
Status of the quantity of heating bath fluid.
Insufficient liquid is detected by liquid level
sensors in the bath. If insufficient liquid is
detected, more oil is pumped into the bath.
Oil Bottle Level
Status of the quantity of heating bath fluid
in the bottle. Insufficient liquid is detected
by a liquid level sensor in the bottle.
Wash vol.
Status of the water volume of the deionized
water bottle. Insufficient liquid is detected
by the deionized water bottle level sensor.
Abnormal indicator and corrective
indicator
OK
OK
OK
3000 -
5000
mL/min.
OK
OK
OK
action
NG
Check the level of heating bath fluid and
replenish it, if needed.
NG
Check the level of bath oil and replenish,
if needed.
NG
Make sure that there is a sufficient supply
of bath oil. If needed, add more oil to the
reaction bath oil bottle.
<3000 or >5000 mL/min.
Fill the incubation bath oil bottle with
more oil. Adjust the flow regulator valve.
Make sure the bath oil is circulating
properly in the constant temperature bath.
NG
Check the level of heating bath fluid and
replenish it, if needed.
Check the bath oil bottle.
NG
Fill the incubation bath oil bottle with
more oil. Make sure the liquid level
sensors are positioned correctly.
NG
Fill the pure-water (deionized) bottle.
Deionizers produce less water at colder
temperatures. If the temperature is lower
than normal, a supply problem may exist.
Also, check your exterior water supply to
confirm that it is adequately supplying the
internal water bottle.
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Operating condition Normal
indicator
Abnormal indicator and corrective
action
Diluent vol.
Indicates the status of the liquid volume of
diluent (saline solution). Insufficient liquid
is detected by a liquid level sensor in the
bottle.
Cuv. Wash Vol.
Status of the liquid volume of cuvette wash
solution. Insufficient liquid is detected by a
liquid level sensor in the bottle.
Cuv. Cond. Vol.
Status of the liquid volume of cuvette
conditioner. Insufficient liquid is detected
by a liquid level sensor in the bottle.
Conc.wast.tank (if installed)
Status of the volume of the optional
concentrated waste tank. A sensor monitors
whether the tank is filled with waste.
Waste tank
Status of the volume of the optional waste
tank. A sensor monitors whether the tank is
filled with waste.
OK
OK
OK
OK
OK
NG
Fill the diluent bottle with 0.9% saline
solution.
Make sure the sensor is in the proper
position.
NG
Fill the cuvette wash solution bottle.
Make sure the sensor is in the proper
position.
NG
Fill the cuvette conditioner bottle. Make
sure the sensor is in the proper position.
NG
Empty the concentrated waste tank.
If there is still a problem, check the tubes
for obstructions.
NG
Verify that the waste tubes are free of
obstructions. If the problem persists,
contact your local support provider or
distributor.
3. Checking the availability of the reagents and wash solutions.
Visually check the system reagents.
a. Perform a prime after replacing any system ancillary reagents.
b. At the Operation Panel, select
c. Select
PRIME 2, then select Execute.
Prime.
d. Visually check the ISE reagents.
e. Perform a prime after replacing the ISE Buffer:
(1) At the Menu Panel, select
Maint., then select ISE Operation.
(2) In the Times box of the Bufferprime area, type the number of prime cycles
you want (
(3) Select
20 is recommended).
Execute.
f. Visually check the controls and calibrators on the calibrator/control tray.
g. Visually check the wash solutions.
h. Visually check the lamp coolant level.
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i. Check the method reagents in the reagent trays:
(1) At the Menu Panel, select
Reagent, then select Reagent Inventory.
(2) At the Reagent Inventory window, determine if any reagents need
replenishing.
(3) Replace any expired reagents.
IMPORTANT
Do not move reagent containers on RTT1 or RTT2 after a barcode scan has been
performed. This can cause erroneous results.
If the operator accidentally switches barcoded reagents (R1 reagent is loaded on
RTT-2 and R2 reagent is loaded on RTT-1) and performs a reagent barcode scan,
and error message displays to alert the operator.
j. Replace barcoded reagent containers as follows:
1. Place the reagent container(s) in any empty position.
2. For multiple reagent methods, place R1 on reagent tray 1, and place R2 on
reagent tray 2.
3. Load multiple containers for each reagent on one tray.
A maximum of 8 reagents can be loaded for the same method.
• Replace nonbarcoded reagent containers as follows:
1. Place the reagent container(s) in the same position.
2. For multiple reagent methods, place R1 on reagent tray 1, and place R2 on
reagent tray.
You can load more than one container for each reagent. You must specify the
reagent at the System Test List window.
3. After replacing the reagent(s), you must establish the correct on-system
stability.
a. Minimize the Reagent Inventory window.
b. At the Menu Panel, select
Settings
.
Reagent, then select Reagent Container
c. At the Reagent Container Settings window, locate the replaced
nonbarcoded reagent.
d. Enter the correct reagent Exp. date in the form YYYYMMDD.
e. Select the
O (Open) button to open the replaced reagent container and
initialize the Days Remaining counter.
NOTE: If you replace only 1 of the reagents of a 2-reagent method and
the replaced reagent has a new lot number, the system alerts you that a
reagent pair does not exist. You should change both reagents at the same
time. If the 2 reagents that you replaced have new lot numbers, you must
re-calibrate the new set of reagents before you continue to run samples.
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(1) At the Menu Panel, select Reagent, then select Reagent Inventory.
(2) Execute a Barcode Scan at the Reagent Inventory window.
(3) Evaluate calibration status.
4. Perform a start-up wash.
WASH 3 or Startup wash, 26 minutes
CTT position 51 DI water (tube)
RTT1 position 50 DI water (70-mL container)
RTT2 position 50 DI water (70-mL container)
WASH 2 or Shutdown wash, 38 minutes
CTT position 15 ISE detergent (cup)
CTT position 16 DI water (tube)
CTT position 49 10% cuvette wash (tube)
CTT position 50 DI water (tube)
RTT1 position 49 10% cuvette wash (70-mL
container)
RTT1 position 50 DI water (70-mL container)
RTT2 position 49 10% cuvette wash (70-mL
container)
RTT2 position 50 DI water (70-mL container)
Weekly wash set up
Same as WASH 2, except replace the 10% cuvette wash with 5%
reagent probe wash in both the CTT and RTT positions.
Times required to perform prime, washes, and cell blank
Typical times
Operation Cycles Time (minutes)
PRIME1 5 1:40
WASH1 1 9:10
WASH2 2 38
WASH3 1 26
Cell blank NA 14
Times for combined operations during startup and shutdown
Startup
• PRIME1 + WASH3 = 28 minutes
• PRIME1 + WASH3 + Cell blank = 42 minutes
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Shutdown
• WASH2 + System End + Power OFF = 39 minutes
• Automatic startup
• PRIME1 + WASH3 = 28 minutes
• PRIME1 +WASH3 + Cell blank (wait for CB Temp) = 72 minutes
• PRIME1 + WASH3 + Cell blank (no wait for CB TEMP) = 42 minutes
Daily Operation
Using workorders
Using host computer workorders
1. Create the workorders at your host computer.
Each patient sample must have a workorder that contains a sample number and at
least one test request.
NOTE: Stop here if you do not want to download workorders manually from the host
computer before operation.
Instead, use the Automatic item select feature in the Automatic transfer area on the
Online Settings window.
2. Download workorders to the chemistry system:
a. At the Menu Panel, select
b. Select
c. In the Entry format area, select the means for identifying the first workorder in
d. In the Last no. entry format area, select the means for identifying the last
e. In the Start no. box, identify the first workorder you want downloaded.
f. In the Last no. box, identify the last workorder you want downloaded or enter the
g. Select Execute.
After the workorders are downloaded, you can manage them in the same way as the
system workorders.
If a workorder already exists for the sample, the host workorder is used. See
managing host and system workorders for details.
Host request.
step 2e.
workorder in step 2f.
number of workorders you want downloaded.
Request, then select Order Entry.
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Creating workorders at the analyzer
1. At the Menu Panel, select Request, then select Order Entry.
2. Select
Routine or Interr.
3. In the Posi.no. boxes, enter the sample position number.
4. In the Samp.no. box, enter the sample identification number.
5. Verify that the System Dilution Mode, Container Type, Sample Type, Dil. factor,
Sex, and Blood collection date entries are correct.
6. As needed, provide entries for Comment and Age.
7. Order tests by any of the following methods:
• In the Test table, select each test or ratio you want to run.
• In the Test-tbl no. box, enter the number of each test you want, then press the
period (.) key.
• In the Profiles area, select each profile you want to run.
8. To de-select all tests, select
9. To confirm, select
Yes.
10. To erase all entries, select
11. To confirm, select
12. Select
Enter.
Yes.
Delete Tests.
Batch Func.
The Number of workorder box increments. Automatic incrementing is assigned using
the Entry Setup window. If autoincrement is on, a new workorder displays with the
next sample number and position number incremented.
13. You can create another workorder or select
If necessary, select
New to clear the window for entry of the next workorder.
Exit to leave.
Creating multiple workorders
1. At the Menu Panel, select Request then select Order Entry.
2. Enter information for the first sample:
NOTE: Enter only those items you want replicated.
a. Select New located above the Enter.
b. Select Routine or Interr.
c. In the Posi.no. boxes, enter the starting sample position number (Tray and Cup
numbers).
d. In the Samp.no. box, enter the starting sample identification number.
e. Verify that the System Dilution Mode, Container type, Sample type, Dil. factor,
Sex, and Blood collection date entries are correct.
f. As needed, provide entries for Comment and Age.
g. Order tests by any of the following methods:
Operating the System 73
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• In the Test table, select each test or ratio you want to run.
• In the Test-tbl no. box, enter the number of the test you want followed by a
period (.).
• In the Profiles area, select each profile you want to run.
3. Select
4. Select
Batch Entry.
Samp.no., Posi.no., or Batch entry button, then enter corresponding
information in the selected box.
5. Select
NOTE: The Posi.no. and Samp.no. fields increment by the number of workorders
requested from Batch Entry.
Execute.
Deleting or changing test selectivity for multiple workorders
1. At the Menu Panel, select Request then select Order Entry.
2. Select
3. Perform any of the following tasks at the window:
4. In the Entry format area, select the means of identifying the first workorder in step 6.
5. In the Last no. entry format area, select the means of determining the last workorder
6. In the Start no. box, identify the first workorder you want changed.
Batch Func.
a. To erase workorders, select
Delete workorder.
b. To change the test selectivity, select
in step 7.
Test correct.
7. In the Last no. box, identify the last workorder you want changed or type the number
of workorders you want changed.
8. If Testcorrect was selected in step 3, select
Test Table.
a. To add a test, select it.
The check mark must appear bold.
b. To delete a test, double-select it. The check mark must appear dim.
9. Select
10. Select
Return.
Execute.
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Creating a profile
1. At the Menu Panel, select Request, then select Order Entry.
2. Select
Create Profile.
3. In the Profile set no. box, enter the profile number (1 to 150).
4. In the Comment box, enter appropriate text.
Because the first 8 characters are used to identify the profile on the Order Entry
window, you can use these characters for the profile name, and the remaining space
for any additional information.
5. Select
Test table.
6. Select each test you want in the profile, then select
Select buttons below the Test table to get more tests.
7. Select
Execute.
Creating a load list
NOTE: A load list contains only workorders that have position numbers. To include
workorders currently without position numbers, use Creating List.
1. At the Menu Panel, select Request, then select Order Entry.
2. Select
Create List.
Return.
3. Select
4. If you selected
All or, if you want to specify a range of position numbers, select Posi.no.
Posi.no., define the tray and cup position.
a. In the Last no. entry format area, select the means of determining the last sample
in step 4c.
b. In the Start no. box, enter the position number for the first sample in the load list.
c. In the Last no. box, enter the position number for the last sample in the load list
or type the number of samples you want in the load list.
5. Select
6. To print the load list, select
Execute.
The system creates the load list and displays it at the
Print.
Worksheet window.
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Loading patient samples
Loading patient samples on the STT
BIOHAZARD
Wear personal protective equipment.
Use universal precautions.
NOTES
Do not stare into the beam.
LASER WARNING
Before starting a run, you must load control and calibrator samples on the onboard
sampler.
To load patient samples while the system is sampling, select the SMP Pause button at the
Operation Panel or on the analyzer.
1. Remove the sample tray evaporation cover.
2. Remove any completed samples and dispose of them in accordance with laboratory
procedure.
3. Make sure that on the Operation Panel, the Load Status Indicator line is green and
that SAMPLE LOAD OK displays
4. Load samples while the sample tray is in the sampler, or remove the tray.
To remove the sample tray, release the safety latches at position numbers 49 and 70,
then grasp the sample tray by its handles and lift it out of the sampler well.
5. Loading the samples.
a. When using barcode labels:
• Clean and properly position barcode labels on the sample tube.
• Make labels face outward and visible through the slot in the sample-tube
holder.
• Labels on tubes loaded in the inner ring (positions 43 through 84) must be
visible between the sample-tube holders in the outside ring.
b. When not using barcode labels, you must load each sample into the sample
position number entered on the workorder.
c. Load sample cups into a plastic adapter.
This adapter can hold two cup sizes. If the cup does not fit, remove the adapter
and try the other end.
76 ADVIA 2400 Operator’s Guide
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d. Using barcode labels with a sample cup, insert the sample cup into a sample tube
that has the correct label.
You can use Ezee Nest cups in primary sample tubes.
CAUTION
Make sure that all sample containers (including tube-cup combinations) are defined
at the System Specification Settings window to avoid probe crashes.
6. Replace the sample tray if it was removed in step 3 and press down on the locking
pins to secure the tray.
7. Replace the sample tray evaporation cover.
CAUTION
Seat the STT cover within the two alignment pins. The dilution probe access holes
must be at the back, and the arrow labels must be aligned next to each other to avoid
probe crashes.
Loading patient samples on the optional universal rack handler
BIOHAZARD
Wear personal protective
equipment.
LASER WARNING
Only field personnel trained by
Siemens should access laser assemblies.
Use universal precautions.
IMPORTANT
Define container types for use on either the universal rack handler (LAS) or the onboard
sample tray (STT).
When you use the same sample rack on the rack handler and on an ADVIA Centaur
system, select allowable tube sizes from the table below. You must use the same tube size
for every tube in a rack.
Tube Type Size Manufacturer Description
Ht+Sample cup
Hitachi
13 x 38 Hitachi Conical bottom in 16 x 15 round
bottom holder
Small transfer 12 x 75 Various Round bottom
Large transfer 16 x 100 Various Round bottom
Because the aspiration positions for the rack handler and the sample tray may be at
different heights, each container type must be defined separately in each sampling mode,
LAS, or STT. That is, if the same container is to be used on the rack handler and the
sample tray, it has to be defined as two different container types, one for LAS and one for
STT. The system defaults to container set #1 when a container is not specified with the
host workorder.
Operating the System 77
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NOTE: If you are running patient samples on the onboard sample tray, you must load
the samples before starting the run. On the optional universal rack handler, load the rack
carrier on the infeed tray and push the Start/Pause switch to start the run. Control and
calibrator samples must be loaded on the onboard sampler (STT).
1. If the rack handler is not turned on, set the rack handler READY/STANDBY switch
to
READY.
2. At the Windows desktop, double-select the rack handler icon.
You must use barcode labels to identify each sample. (Position numbers cannot be
used.) Properly affix each barcode label and make it visible through the slot in the
rack.
Figure 2-2. Loading sample onto rack
3. Load the racks with the sample tubes and slide the loaded racks onto the rack carrier.
CAUTION
You must have a unique barcode label on each rack to avoid universal rack handler
user errors.
If you use the same sample rack on the rack handler and on an
ADVIA Centaur system with the same ID labels but different series numbers (2, 3,
4), the rack handler reads the barcodes as duplicate rack IDs. This can cause software
errors.
4. Load racks onto the carrier.
5. Lift the carrier by the handle and place it on the infeed tray
6. Push the Start/Pause switch to start the rack handler.
CAUTION
If for any reason you must remove the carriers manually from the system, do not
push the carriers back into the outfeed buffer.
When the top cover is raised and the infeed pusher arm is in the up position, avoid
injury by bending over and coming into contact with the pusher arm.
78 ADVIA 2400 Operator’s Guide
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7. For continuous loading of new rack carriers, push the Start/Pause switch and hold it
for ten seconds until the rack handler status display reads Continuous feed.
When the barcode reads a label, the corresponding sample indicator turns green.
The sample indicator turns red if the sample tube is detected, but the barcode label
cannot be read. If the sample tube is not detected, the sample indicator will not glow
green or red.
Urgent samples and manual reruns are run from the onboard
sampler (STT)
1. Verify that a workorder or rerun request exists for each STT sample.
2. At the Operation Panel, select
The operation mode changes to Pause Shift, then to Pause. A short delay may occur
while the analyzer changes operational states.
3. Load samples on the STT.
4. At the Operation Panel, select
5. Complete the Start Conditions window and start the run.
Make sure to select the
After aspirating the STT samples, the system resumes sampling from the rack
handler if racks are available, or it will enter the Watch mode while waiting for the
next rack.
Starting the run
WARNING
If the system was stopped using the Emergency Stop button, you must perform a
Weekly wash (WASH2) prior to processing samples.
1. In the Operation Panel, select
Pause.
Start.
Analyze box for the onboard sampler.
Start.
The Start Conditions window displays.
2. Perform any of the following tasks on this window:
a. run calibrators and reagent baselines (RBL)
b. run control samples
c. You cannot run multipoint calibrations, while you are running patient samples
from the STT tray.
d. Run patient samples:
e. from the STT
f. from the optional universal rack handler or external transport
3. To begin the run, select
Operating the System 79
Start.
Page 80
Running calibrators and reagent baselines (RBL)
1. Select Multipnt.smp. Analyze, then select TT No. 98 or 99 to select the STT tray
number.
2. Select
One-pnt.smp. Analyze.
You can select both calibration types.
3. Select
4. To remove any tests you do not want calibrated in this run, select
5. To remove any calibrators you do not want used in this run, select
Ordinary calib. or Special calib.
.
select
Temp.item select.
Temp.sample
To run only a reagent baseline (RBL) or blank rate, select only the blank solution.
Running control samples
1. Select Control smp. Analyze.
2. To remove any tests you do not want run on the controls, select
3. To remove any controls you do not want used in this run, select
.
select
From STT
1. Make sure the samples are loaded.
2. In the Ordinary sample area, next to Routine smp, select
Analyze.
This activates other fields.
Temp.item select.
Temp.sample
3. To specify how the samples are identified, in the Ordinary sample area, next to
Analyze mode, select
If you select
Barcode, the system scans the entire STT tray for barcodes.
Barcode or Cup posi.
You can choose to scan for a single tray position or for a range of positions as
follows:
4. In the boxes to the right of Analyze, enter the range of positions you want to scan.
For example: If you enter 50 and 60. The system scans for barcodes within this range
and will aspirate only from positions at which it found barcodes.
If you want the system to aspirate sample from a specific position or specific range of
positions, select
Cup posi.
Enter the tray number.
In the boxes to the right of Analyze, enter the position or range of positions from
where you want the system to aspirate samples.
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5. Select Temp.cup/tube select to change container types and select samples for
priority processing.
You can select more than one position at a time and to assign the same container type
to all.
6. To begin the run, select
Start.
From the optional universal rack handler or external transport
1. Select the second General smp. Analyze box (below Out side analyze).
2. To begin the run, select
Start.
3. Load the racks on the rack handler or the universal rack handler.
Running an interrupt (STAT) sample
Running an interrupt sample when sampling is in progress (START)-SMP
LOAD NG
1. Download or create the sample workorder and select
workorder.
NOTE: You can also select the priority using the Cup/Tube Assign window if the
sample is already on the sample tray and already has a workorder.
2. At the Operation Panel, select Pause to halt sampling.
3. Load the new sample on the sample tray (STT).
4. At the Operation Panel, select
Start to resume sampling.
Running an interrupt sample when sampling has stopped (STOP)-SMP
LOAD OK
Interr. (interrupt) option on the
1. Select Interr. (interrupt) option on the sample workorder.
2. Load the sample on the sample tray (STT).
NOTE: Priority can be set using workorder Interr. designation and the Cup/Tube
Assign or Sample confirmation window that displays after reading the barcoded
samples.
3. To begin sampling, at the Operation Panel, select
Start.
Operating the System 81
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Monitoring an analysis while it is running
1. At the Menu Panel, select Request, then select Test Result Monitor.
2. Monitor any of the following at this window:
a. processing status
b. sample information and system status
c. result data information
When the run is complete, the operating mode display becomes END, then returns to
READY if you ran the samples from the STT. The system returns to WAIT if you ran
samples from the rack handler or lab automation system.
3. Close the window.
Processing status
The center of the window resembles the sample tray. The outer ring is the STT tray and
the inner ring is the CTT tray. Each tray position is a circle.
As the run continues, the circles containing samples change color. The colors indicate the
current status of each sample. The color codes are displayed at the lower-left of the
window.
For additional information about the color codes see page 41.
Sample information and system status
The button bar at the top of the window displays the Sample Search and Rack or LAS.
Sample Info. buttons.
• Select
• If a rack handler or laboratory automation system (LAS) is in use, select the
Sample Search to display a dialog box where you can search for sample
information by sample number by STT position (tray number and sample position),
or by rack/LAS position (rack number and sample position).
The search returns a Sample Information window showing the sample number,
position number, sample status, and the time remaining to complete the analysis of
the sample.
Rack or
LAS. Sample Info. button to display the following sample information in the Rack or
LAS. Sample Information window:
♦ Sample barcode number.
♦ Sample status (see the status codes listed on the Test Result Monitor window).
♦ The time remaining to complete the processing of the sample.
NOTE: If processing has completed or if there are no samples available for
processing when you select the Rack or LAS. Sample Info. button, the system
displays a message that no sample information is available.
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The left side of the Test Result Monitor window displays three panels.
• The System Status panel shows the current operating mode of the system.
• The Sample information panel shows the sample number and sample position of the
currently selected position on the STT/CTT graphic. If this is a barcode analysis, the
barcode number displays, and the position number is 0-00.
• The code panel shows the color codes used in the STT/CTT graphic to represent
sample status.
In the middle of the sample tray display, the tray (TT) number for the current run displays
in the TT No. list box. If this is a barcode analysis, the TT number is 0.
To view the status of prior tray samples that are still in process, select the down arrow of
the TT No. list box, then select the number of the tray you want to view.
Monitoring samples loaded on the optional universal rack handler
1. At the universal rack handler Operation Panel window, select View on the menu bar,
then select
Perform any of the following tasks at the Rack Handler Operation Monitor window:
a. follow the progress of each rack through the rack handler
b. locate racks that contain sample tubes with non-read labels
Rack Handler Operation Monitor or press F8.
c. find a specific rack or tube currently on the rack handler
d. skip un-sampled racks
e. clear rack ID numbers where non-read ID samples occurs
f. edit non-read tube IDs
Perform the following tasks at the universal rack handler:
a. enter a sample ID for non-read labels at the Edit Non-Read Tube ID window
b. obtain a summary of the current rack handler activity at the Data Monitor
window
c. view and print all the racks/samples processed by the rack handler on a day-by-
day basis at the Review Data window
2. Use the Review/Edit window on the ADVIA software to determine when results are
available.
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Using the Sample Log
A
1. At the Menu Panel, select Request, then select Sample Log.
2. Perform any of the following tasks at this window:
a. view the sample log entries
b. search the sample log
c. delete a specific sample log entry
d. delete all sample log entries
e. print a list of the sample log entries
f. export the sample log entries
3. Close the window.
Viewing the sample log entries
1. In the list box, select the file containing the sample log entries you want displayed.
The file for the current day is selected automatically.
2. Select the types of sample entries you want to display:
a. Select
b. Select
c. Select
ALL to view all sample entries.
Routine/Ctl to view patient and control sample entries.
Routine Only to view patient sample entries.
The following information is provided for each sample log entry:
sp Date and Time External Type
STT SampleID Rerun
CTT Description Results
3. To view entries for recently aspirated samples, select
Update.
Searching the sample log
1. Select the search condition(s) you want and enter the applicable information:
Sample ID: Enter the sample identification. Search is case sensitive (C0101 is not the
same as c0101).
Asp. Date: Enter the aspiration date using the format YYYYMMDD where YYYY
is the year (2006), MM is the month (01 to 12), and DD is the day (01 to 31).
Asp. Time: Enter the aspiration time using the format HH:MM:SS where HH is the
hour (00 to 24), MM are the minutes (00 to 60), and SS are the seconds (00 to 60).
You can enter an approximate aspiration time (for example, 14:00:00), but the time
entry must be complete (that is, 14:00 causes an error, it should be 14:00:00).
2. Select
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Execute.
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Deleting a specific sample log entry
1. Select the sample log entry you want to delete.
2. Select
3. To confirm, select
Clear.
Yes.
Deleting all sample log entries
1. Select All Clear.
2. When the All Clear dialog box opens, do one of the following:
a. If you want to delete all entries for a specific day, select
Day.
b. In the list box, select the file containing the sample log entries you want to delete.
The file for the current day is selected automatically.
c. If you want to delete the entire sample log, select
3. Select
4. To confirm, select
OK.
Yes.
All.
Printing a list of the sample log entries
1. At the File menu, select Print Setup.
2. Verify that all printing options are correct, then select OK.
3. Select
Print Log Summary.
4. Verify that the aspiration dates and times represent the range of sample log entries
you want printed.
a. Change an aspiration date using the format YYYYMMDD where YYYY is the
year (2006), MM is the month (01 to 12), and DD is the day (01 to 31).
b. Change an aspiration time using the format HH:MM:SS where HH is the hour
(00 to 24), MM are the minutes (00 to 60), and SS are the seconds (00 to 60).
5. Select
Print.
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Exporting the sample log entries
You can export the current sample log file in ASCII format for use by another program
such as Microsoft Excel.
1. At the File menu, select
2. Select or create the folder in which you want to save the file.
3. In the File name box, type the file name you want.
The sample log file name is entered by default.
4. In the Save as type list, select the file format.
The CSV (comma separated values) file format is selected by default.
5. In the Deliminator list, select the character used to separate sample log data items.
6. Select
Save.
Export.
Reviewing the calibration results
• Use the View Calibration Curve window to review the calibration details for
photometric methods.
• Use the ISE Monitor to review the calibration details for electrolyte methods.
See Checking ISE Calibration for information about the criteria used to accept or reject
the ISE calibration.
Reviewing the sample results
Sample results can be reviewed on and report from the RealTime Monitor and
Review/Edit windows.
Reporting results
The system prints calibration, control, and patient sample results. Only patient and
control sample results are transmitted to a host computer.
Results are reported automatically as the sample analysis is complete and results become
available, or the operator can manually select results to report in batches.
Real-time reporting
• During operation, the System set area of the System Monitor window manages the
printing of the sample results (usually for laboratory purposes).
• The Automatic transfer area of the Online Settings window manages the real-time
transmission of the control and patient samples to a host computer.
• Use the Data Clean feature of the Online Settings window to prevent the transmission
of questionable sample results. The Transfer Results List dialog box of the
Review/Edit window indicates which samples failed this data check and were not
transmitted.
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Batch transmission to a host computer
Use the Review/Edit window to transmit selected patient results. The data clean feature is
available to check the sample results for problems.
Printing sample data
The Print report window is only available from the supervisor logon. To print reports, go
to the Review/Edit window and use the Print report button.
CAUTION
Tests on the Print Report form must be in the same order as they appear at the System
Test List window, to avoid incorrect positioning of results on the print report. Do not
modify the order of the test names at the System Test list window after the initial
setup. Any repositioning of test names on the System Test list window could result in
associating already-run tests with the wrong test name.
1. At the Menu Panel, select
2. At the Review/Edit window, select the
Request, then select Review/Edit.
Print Report button.
3. At the Print Report window, select a Sample Type(T) and File Data(F).
a. If you select Archive in the File Data (F) field, the Filing File Name (I) field is
activated.
b. Select the
Open button.
c. Select a file name at the Open window.
4. In the Specification range (R) field, select the
Range
or All from the list.
5. In the Number entry format (N) field, select the
arrow in the field and select Input
arrow in the field and select a format
from the list.
6. In the Last no. entry format (E) field, select the
arrow in the field and select a format
from the list.
7. In the Report Form File (O) field, select a print format for the report.
a. Select the
Open button.
b. The Open window displays, defaulting to the ETC folder.
c. Two sample report formats are available:
• the Chart.frm file is formatted for a single sample.
• the Consoled.frm file is formatted for multiple samples.
d. Customer defined print formats also display in the folder.
e. At the Open window, select a file name for the report format.
f. Select Open.
8. Enter a start number in the Start number (S) field and a last number in the Last
number (L) field.
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9. To print the report, select the
Print button.
10. Close the window.
Batch Printing
Use the Review/Edit or Print Report windows to print selected patient results.
Print Format Settings window can create up to ten report layouts.
The
Shutting down the ADVIA Chemistry system
NOTE: When an automatic shutdown is performed, a "1903 system error ... Shut down
the system and reboot it." message is generated to the system log. Ignore this message.
1. At the System(S) drop-down list of the Menu Panel, select Exit.
2. When the confirmation message box displays, select
3. When the second confirmation message box displays, select
Yes.
Yes.
After a brief delay, the Startup window displays.
4. Select
Shutdown.
After a brief delay, the message box opens to allow you to turn off the computer.
5. At the power panel, set the Operate/Standby switch (
The power indicator (
2) is off.
1) to Standby.
Figure 2-3. Power panel at Shut down
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Examples of shutdown settings
The following examples demonstrate some common scenarios that you can implement
with the Shutdown Set feature of the System Startup/Shutdown Settings window.
System available during the night
To have both the computer and analyzer available for night emergencies, use the
following:
System p.s. Power OFF check box is cleared (no check mark).
System Select
PRIME Select
WASH1 Do check box is cleared (no check mark).
WASH2 Do check box is selected (check mark present).
WA SH 3
Automatic startup from the Sleep state
In the Sleep state, the Power Panel Operate/Standby switch (1) remains set to OPERATE.
However, electrical power is supplied only to the necessary components, and the Power
indicator (
To place system in the SLEEP state, use the following:
System p.s. Power OFF check box is selected (check mark present).
System Select
PRIME Select
WASH1 Do check box is cleared (no check mark).
WASH2 Do check box is selected (check mark present).
WASH3 Do check box is cleared (no check mark).
Do check box is cleared (no check mark).
2) is off.
Not do.
None.
System end.
None.
Automatic startup with analyzer power kept ON
To keep analyzer power on until the automatic start up, use the following:
System p.s. Power OFF check box is cleared (no check mark).
System Select
PRIME Select
WASH1 Do check box is cleared (no check mark).
WASH2 Do check box is selected (check mark present).
WASH3 Do check box is cleared (no check mark).
NOTE: If System is set to Not do, you must select System end in the System list before
the automatic startup setting time occurs. Otherwise, the automatic startup does not
occur.
In this case, you cannot shut down Windows even if you select Shutdown on the
[ADVIA] Startup window. Additionally, the CRT screen saver does not operate.
Not do.
None.
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Performing a system-assisted shutdown (Shutdown set)
1. Verify that sufficient system reagents and wash solutions are available for the startup
you want to run.
2. At the Menu Panel, select
3. In the Mode set list, select
Maint., then select System Startup/Shutdown Settings.
Shutdown set.
4. Verify the selections for the shutdown you want to run.
If no shutdown is defined or you want to change an existing one, proceed as follows:
a. For each of the Proc. set, Set1, Set2, or Set3, you can select one of the following:
(1) In the System p.s. area, if you want the system to turn off analyzer power
after the shutdown is completed, check the box next to Power OFF.
(2) In the System end area, select the software shutdown mode you want.
(3) In the PRIME area, select the prime you want to run or select
NONE.
A Prime is required only if a system reagent is replenished or a related
component is replaced.
(4) Select the check box next to each wash you want to perform.
WASH2 for the shutdown wash.
Select
b. Select
c. To confirm, select
5. In the Select wash routine area, select
Save.
Yes.
Shutdown.
6. In the Proc.set list, select the shutdown setting (Set1, Set2, or Set3) you want to run.
7. Verify that the system is in Ready mode.
8. In the Start selected routine area, select
Yes.
The current operating mode displays in the System mode box, and the time remaining
to complete the shutdown displays in the Time until end box.
9. To halt the shutdown, do the following:
a. At the System Startup/Shutdown Settings window, select
b. In the Start instructions area, select
c. At the Operation Panel, select
NOTE: To avoid entering the Wait state and not shutting down properly, do not use the
Cancel.
Stop.
Exit command from the System(S) menu on the Menu Panel.
Stop.
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Additional Operating Instructions
To avoid errors or interruptions during operation, please read the following before using
the Chemistry System.
Switching Process Sequence and System Test List numbers
To avoid having quality control data reported for the wrong tests, do not switch
Process Sequence Numbers
results are also affected.
To avoid incorrect reporting of results at the Review and Edit, the Daily QC windows,
and the Print Report form,
System Test List window after initial setup. If you reposition any test items at the System
Test List window, results for samples already run could be associated with the wrong test
name.
Loading the reagent trays
CAUTION
Make sure that each reagent container (wedge) is loaded on the correct reagent tray to
avoid erroneous results when using reagent containers without a barcode. Reagent Tray 1
(RTT-1) is the front tray, while Reagent Tray 2 (RTT-2) is the rear tray.
after control results have been accumulated. Patient
you must not modify the order of the test items at the
The operator is alerted to this problem by the QC results.
Corrective action is to reload the reagents on the correct trays.
Updating the Cumulative QC
Update the QC Cumulative window before more than 200 daily control samples are run.
The QC Daily Precision Control window can manage a maximum of 200 results for each
control product (level). If an additional control sample is run, the current 200
(Px200) is overwritten by the new control result. Similarly, if multiple repetitions of a
control are requested, the system aspirates only one sample and its result replaces the
existing 200
th
result (Px200).
To avoid losing control results, you must perform a "QC cumulative save" at the QC
Daily Precision Control window at the end of each day, and perform a New Start when
the system is turned on the next day.
Do not update the QC Cumulative window more than once each day.
If you return to the QC Daily Precision Control window after performing a "QC
cumulative save," you must not perform another update.
Specifically,
you must not select Yes when prompted by "Add daily data to QC
cumulative data." If Yes is selected and there is no daily control data available, the
cumulative data point will be deleted for the day and no additional control data can be
saved for the day.
th
result
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Running automatic calibration
Controls requested at the Start Conditions window are run before the automatic
calibration samples.
You can use the Auto Calibration Setting dialog box at the Calibration Setup window to
request automatic calibration after a specific time interval or after a new reagent
container is loaded. If the calibration interval expires while the system is not running
samples, the automatic calibration is performed at the beginning of the next run.
However, if the calibration interval has expired and
controls are requested at the Start
Conditions window, the controls are run before the automatic calibration.
At the start of a run, calibration and control samples are run using the following priority
order:
1. Manual calibrations requested at the Start Conditions window
2. Manual controls requested at the Start Conditions window
3. Automatic calibrations requested on the Auto Calibration Setting dialog box
4. Automatic controls requested on the Auto Control Setting dialog box
Using multiple reagent containers for the same test
You can use multiple, barcode-labeled reagent containers for the same test by loading the
reagent containers on Reagent Tray 1 (RTT-1) or Reagent Tray 2 (RTT-2).
After the reagent barcode labels are read, the reagent container with the lowest reagent
tray number for each test is used first. If that reagent container is empty, the reagent
container with the next lowest reagent tray number is used.
Before performing a reagent barcode read or a reagent reset, you should remove any
empty reagent containers from the reagent tray.
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3 Calibration
Calibration overview
When to calibrate
Refer to the ADVIA Chemistry system methods documentation for the calibration and
the reagent blank/rate recommendations.
Setting up the calibration
• Use the Calibration Setup window to set up calibration for the photometric methods.
• Absolute and single-point calibration samples are aspirated from the
calibrator/control tray (CTT), while multipoint calibration samples are aspirated from
the sample tray (STT). The blank solution (water) used to run the reagent blanks/rates
is typically assigned to CTT-1.
• For IgA, IgG, and IgM methods only: Use the Multi-Standards Set Calculator to
determine standard values. Refer to the IgA, IgG, and IgM method sheets for
instructions about setting standard values and calibrator handling and preparation.
NOTE: If you are running IgA_2, IgG_2, or IgM_2, this does not apply.
• For multi-point calibration methods, simplified calibration is available after a valid
multi-point calibration is performed.
• Use the ISE Parameter Settings window to set up calibration for the electrolyte
methods.
ISE Calibration samples are aspirated from the calibrator/control tray (CTT):
serum - low cal, CTT-11; high cal, CTT-12
urine - low cal, CTT-13; high cal, CTT-14
Entering absolute or single-point (STD) calibration methods
1. At the Calibration Setup window, in the Proc. Test No. list area, locate the applicable
method.
2. In the Blk posi. Box for the method, enter the cup position for the blank solution on
the calibrator/control tray (CTT).
3. In the STD posi. Box, enter the cup position for the calibrator on the
calibrator/control tray (CTT).
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4. In the Coeff (FV) box, enter the applicable value.
NOTE: Do not enter the factor values in the View Calibration Curve window at this
time. These values may not be saved. Instead, enter the factor values at the
Calibration Setup window.
5. Select Ctrl/Cal Setup.
6. Locate the cup positions occupied by the blank solution and the calibrator.
7. For each item, do the following:
a. At the container Type, select the correct tube or sample cup.
b. In the Meas. Times box, enter the number of aspirations you want taken.
c. In the Comment box, enter the applicable text that describes the blank or
calibrator.
d. Close the winnow.
8. At the window button menu, select
9. To confirm, select
Yes.
Save.
Entering multi-standard calibration methods
1. At the Calibration Setup window, in the Proc. Test No. list area, locate the applicable
method.
Multipoint calibration methods are identified by a Setting button in the MSTD
column of the Procec.test no. list.
2. To view the Multi Standard Setup window, select
3. In the TT no. area, select
98 or 99.
4. In the Posi. Box of the Blank row, enter the cup position for the blank solution.
5. For each standard do the following:
a. Using the information in the calibrator package insert, enter the Lot No., Lot
Name, and Exp. Date.
b. In the Posi. box, enter the cup position of the standard.
c. This is the location of the calibrator on the tray.
d. In the Coeff (FV) box, enter the analyte concentration in the standard.
Setting.
You can get this information from the calibrator package insert or calculate the
value using the standard calculator available on the Method Directory. This
directory may already be loaded with the Operator's Guide. From the table of
contents on your left, select
NOTE: Do not enter the factor values in the View Calibration Curve window at
this time. These values may not be saved. Instead, enter the factor values in the
Calibration Setup window.
e. Select Return.
f. At the Calibration Setup window, select
94 ADVIA 2400 Operator’s Guide
Methods, then select Methods Directory.
Save.
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How to calibrate a multi-standard method
1. At the Analytical Parameter (Chemistry) window, in the Standard Setting area, select
Multipoint Cal Setting.
2. When the Multi-Standards Set window opens, verify that all the parameter
information is already pre-defined.
3. If this is not the case, enter method-specific values from the method's parameter
sheet.
NOTE: The parameters for each method are available on the Method Directory. This
directory may already be loaded with the Operator's Guide. From the table of
contents on your left, select Methods, then select Methods Directory.
4. Add the test name to the System Test List window.
5. Add the test to the Process Sequence window.
6. At the Calibration Setup window, select
calibration information into the Multi Standard Setup window.
7. Select
Return.
8. At the Calibration Setup window, select
9. At the Calibration Setup window, select
Container Type.
10. Select
Save.
11. At the Test Select window, select the test.
12. At the Sample Select window, select the calibrators.
Running calibration
• Use the Start Conditions window to request a calibration run. If needed, you can run
the calibration samples followed by controls and patient samples as appropriate. You
can limit calibration to selected calibration samples or to selected methods.
For example, if you want to run only a reagent blank or blank rate, select only the
blank solution.
• The Ordinary calibration meas. list at the Test Select window determines which tests
are available to calibrate. The Sample Select window determines which calibrators
are available.
• Use the ISE Operation window to manually request an ISE calibration for
maintenance or troubleshooting purposes.
• Use the Calibration Setup window to automatically perform calibration after a user-
specified time interval.
• The Auto calibration meas. and Auto calib. control samp. meas. lists on the Test
Select window determine which tests are available for calibration and control,
respectively. The Sample Select window determines which calibrators and controls
are available.
Setting and enter the multi-standard
Save before continuing.
Ctrl/Cal Setup and define Meas.time and
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Reviewing the calibration
NOTE: For detailed information on how to use the View Calibration Curve and ISE
Monitor windows, refer to the online Operator’s Guide.
• Use the View Calibration Curve window to review the calibration of photometric
methods.
• Use the ISE Monitor window to review the calibration of electrolyte methods.
Calibration/RBL History Window
Use the Calibration/RBL History window to review the calibration history for all
methods that are run at the system and to create CSV files.
1. At the Menu Panel, select Calibration, then select Calibration/RBL History.
2. Select the calibration data to display by selecting Latest or Select:
• Latest–Displays the most recent calibration data for each method on the system.
• Select–Activates the Test Name box to allow a more detailed test selection.
3. If the Select option is chosen, then select one of the 3 Test Name options:
• Photometric–Displays all calibration history data for all photometric methods.
• ISE Tests–Displays all calibration history data for all ISE methods.
• Other–Activates the test list box to allow specific test selection (highlight each
test to display).
4. Specify a date range, to display data for calibrations performed on specific days for
the tests selected:
a. Type the Start Date (yyyymmdd) of the range (if other than the default, which is
the current date).
b. Type the End Date (yyyymmdd) of the range (if other than the default, which is
the current date).
c. Select Search to display the calibration data for the tests selected for the
specified date range.
NOTE: Select and drag the column heading borders to resize the columns in the
calibration data table.
d. To clear the data for the method selected, select Clear.
e. When prompted, select Yes.
5. To reset the calibration select the Reset check box.
NOTE: You cannot cancel the reset once you select Save. If you resize the reset
column an save your changes, those changes are retained when you exit out and back
into the window.
6. Select Print, to print the data displayed.
7. To create a CSV file of the displayed data for archiving, or for exporting the data to
another application, do the following:
a. First, select the data you want to save.
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b. Select Create CSV.
c. Browse and select a folder in which to save the CSV file.
d. Select Save.
8. Close the window.
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4 Quality Control
Quality control overview
When to run control samples
Refer to the ADVIA Chemistry system methods documentation for the quality control
recommendations.
Setting up quality control
• Use the QC Sample Definition window to define each of the 26 controls that can
reside on the calibrator/control tray (CTT).
• Use the Control Data Setup window to enter the control average and standard
deviation (SD) data for each control.
Running the control samples
• Use the Start Conditions window to request control samples. You can run control
samples alone, after calibration, or before patient samples. You can request to run
only specific control samples or selected methods.
• The Ordinary control samp. meas. list on the Test Select window determines which
tests are available to run. The Sample Select window determines which controls are
available.
• Use the QC Sample Definition window to automatically run controls after a specified
number of assays.
• The Auto control samp. meas. list on the Test Select window determines which tests
are available to run. The Sample Select window determines which controls are
available.
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ADVIA QC window
Use this window to view both real-time and long term evaluation of analyzer and method
performance.
Perform any of the following tasks at this window:
• collect control results.
• calculate and display statistical data.
• assess data errors.
• identify QC violations.
• review control results.
• create printed reports.
• identify and report events such as lot changes and calibration dates.
1. At the Menu Panel, select QC then select ADVIA QC.
Reviewing the control results at different windows
In addition to the ADVIA QC you can use the following windows for reviewing QC:
• Use the Real-Time QC window to review control performance during the day.
• Use the Daily Precision Control window to review the control results at the end of
the day, and then to update the cumulative statistics.
• Use the QC Cumulative window to evaluate long-term trends in control performance.
If control results fail to meet the laboratory’s established criteria for acceptability, reevaluate all patient test results obtained in the unacceptable test run to determine if
patient test results were adversely affected.
The laboratory should take and document appropriate corrective actions, which may
include recalibration, before reporting patient results.
Verify that the controls and reagents were prepared properly and have not expired.
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