Installation and Operation instructions
AOC-20i Dual Tower with and without AOC-20s
Figure 1: AOC20i Dual Tower with and without AOC-20s
1.1 Compatibility:
• AOC-20i towers used for any Dual Tower installation must have the same Firmware
generation:
Both must have Version 2.1 or lower (min. 1.8) or Version 3.0 or higher (current
Version is 3.20).
The Version of AOC-20s is independent but must be Version 1.4 or higher (current
version is 3.50).
• On GC-2010 (Plus) independent what software control is used the communication
parameter setting on both AOC-20i towers is F83=001 and F84=000.
• AOC-20i Dual Tower works with internal and external control board.
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1.2 Mounting:
On GC-2010 (Plus) with two analytical lines there are two different arrangements of the
AOC-20i mounting pins (two mounting kits are required; P/N 221-44548-93). AOC-20i Dual
Tower with AOC-20s works only if the injectors are mounted on position 6 and 7.
1) Mounting pin arrangement for one AOC-20i Tower individually used on line one or
two (swapping tower).
Figure 2: Mounting pins for AOC-20i or AOC-20i+s on GC-2010 (Plus) with two analytical lines. Second
picture shows the mounting kit. From left to right the number or the pins in the picture are 4, 2, 1, and 3. In
older mounting kits (as used in the pictures) Pin 4 is identical to Pin 3, in new mounting kits pin 4 has changed..
2) Mounting pin arrangement for one AOC-20i Dual Tower. The rear side Tower is
turned 180° thus the mounting pins have a “mirrored” arrangement.
Figure 3: Mounting pins for AOC-20i Dual Tower with and without AOC-20s
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On the AOC-20i control board (internal or external) the front AOC-20i Tower (Main) must be
connected to “Injector 1”, the rear side AOC-20i Tower (Sub) to “Injector 2” and the AOC20s to “Sampler”
Connect the Start/Ready cables. For ClassVP/EZchrom software it is essential that the two
Ready cables are not connected and that they have no contact closure (isolate them with tape).
For all other software packages they should be connected to the GC Ready out.
2.1 AOC-20i Dual Tower configuration with AOC-20s:
In order to have defined settings of all functions in both AOC-20i a “hard” reset of both AOC20i was performed. Normally the reset in not required but avoids that former parameter
settings on the AOC-20i might cause problems with Dual Tower functionality.
It is not needed to have the rear side (SUB) Tower correctly mounted for the following
procedure. Both towers can be placed as shown below.
Figure 4: Initializing (reset) and function setting on both towers. It is not necessary to do this on the correctly
mounted Dual Tower. Displayed is the Firmware Version F61.
Reset of all Functions of AOC-20i to default settings:
The procedure must be done with both Towers separately
1) Check F84=000
2) In GC-2010 (Plus) “Option / AOC-20i Menu” switch AOC-20i OFF (see Fig. 6)
3) Keep the SAMPLE WASH key pressed and turn AOC-20i ON.
4) Release SAMPLE WASH key and set F60=001; press Enter
All LED on AOC-20i flashes shortly
5) Check F84 again. If it has changed F84=001 the “hard” reset was successful.
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Now the parameter settings for Dual Tower can be made.
Set on both AOC-20i Towers:
F83=001, press Enter
F84=000, press Enter
F97=000, press Enter
After these settings were made on both towers switch them OFF on the GC-2010 (Plus). If not
done already mount the two Towers correctly, and switch them ON again.
For Dual Tower with AOC-20s F97 must be 000, for Dual Tower without AOC-20s F97 must
be 001, on both Towers!
On the GC you need to configure a Dual Line System:
Figure 5: Line configuration GC-2010 (Plus) for AOC-20i dual tower with and without AOC-20s
Press the “Option” button (if necessary several times) until you see the AOC-20i menu on
screen (Fig. 8). Most likely the GC has already recognized the dual tower configuration and
displays the headline “Dual AOC-20i+s” (Fig.8). If the display shows only “Single AOC20i+s” (Fig.6) you need to go into the sampler menu by pressing the PF1 button (in necessary
press toggle button until “Sampler” appears above PF1 button, Fig. 7).
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Figure 6: If GC-2010 (Plus) still displays “Single AOC-20i+s” go into sampler settings
Change sampler settings:
Figure 7: In GC-2010 (Plus) Samper setting set Samper (AOC-20s) and Sub AOC to “Use”
Go back into main menu. Switch AOC POWER Off and ON again (see Fig. 6). Wait for
“AOC link OK” message. In AOC options the GC should display:
Figure 8: Correct display of GC-2010 (Plus) for AOC-20i Dual Tower with AOC-20s. With the PF1 button
(Main/Sub) you can toggle between the Main and Sub Tower parameter settings.
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3.1 AOC-20i Dual Tower configuration without AOC-20s:
Important:
AOC-20s must not be connected to the “Sampler” port of the AOC-20i control board (internal
or external). The GC recognizes the connection and AOC-20i Dual Tower without AOC-20s
cannot be configured!
AOC-20i Dual Tower without AOC-20s is still a special configuration. You might get the
error message “LE2” due to communication problems. This happens if F97 is not set
correctly.
“Hard” Reset
In order to have defined settings of all functions in both AOC-20i a “hard” reset of both AOC-
20i was performed. Normally the reset in not required but avoids that former parameter
settings on the AOC-20i
It is not needed to have the rear side (SUB) Tower correctly mounted for the following
procedure. Both towers can be placed as shown in Fig. 4.
Reset of all Functions of AOC-20i to default settings:
The procedure must be done with both Towers separately
1) Check F84=000
2) In GC-2010 (Plus) “Option / AOC-20i Menu” switch AOC-20i OFF
3) Keep the SAMPLE WASH key pressed and turn AOC-20i ON.
4) Release SAMPLE WASH key and set F60=001; press Enter
All LED on AOC-20i flashes shortly
5) Check F84 again. If it has changed F84=001 the “hard” reset was successful.
Now the parameter settings for Dual Tower can be made. Set on both AOC-20i Towers:
F83=001, press Enter
F84=000, press Enter
F97=001, press Enter
After these settings were made on both towers switch them OFF on the GC-2010 (Plus). If not
done already mount the two Towers correctly, and switch them ON again.
For Dual Tower without AOC-20s F97 must be 001, for Dual Tower with AOC-20s F97 must
be 000, on both Towers!
Make a two line configuration as shown in Fig. 5.
If everything worked in AOC-20i Options the Headline Shows “Dual AOC-20i Main” (and
you can toggle to “Dual AOC-20i Sub” via PF1. In the PF menu of the display you find no
“Sampler” button anymore (see Fig.6) only the “Sub AOC” choice.
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Figure 9: In case of AOC-20i Dual Tower configuration there should be only the choice “Use” or ”Not use”
Sub AOC. Set should be “Use” Sub AOC
If GC-2010 (Plus) still offers the “Sampler” settings (as shown in Fig. 7) select:
Use of Sampler Not used
Use of Sub AOC Use
Bar Code Reader Not Used
In the AOC Parameter settings switch “AOC POWER” OFF and ON again (see Fig. 6). Wait
for the “AOC link OK” message. Now the display should show:
Figure 10: Correct display of GC-2010 (Plus) for AOC-20i Dual Tower without AOC-20s. With the PF1 button
(Main/Sub) you can toggle between the Main and Sub Tower parameter settings.
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Please notice:
If AOC-20i Dual Tower without AOC-20s is configured and you switch GC Power ON the
“AOC-20i link OK” message needs much longer than normal. If you go into the AOC-20i
settings via “Option button” before the “AOC link OK” message appears, the GC displays
“Dual AOC20i+s Main” which is of course wrong. This is corrected as soon as the link is
established. After “AOC-20i link OK” message, go out and then back to the AOC-20i
settings. The headline of the screen has changed to “Dual AOC-20i Main”
4.1 Trouble shooting:
• Default of F97=000. F97 is included in “hard” reset of AOC-20i firmware 3.0 or
higher. It might be not affected by “hard” reset in case of Firmware 2.1 or lower.
Please check!
• For any AOC-20i configuration:
If the first run of a sequence is done properly but then the AOC-20i stops working
most likely on the GC the parameter “Number of Injections” is not set to one.
Unfortunately this parameter is not downloaded by most software packages. If it is
changed on the GC it is not overwritten by software download. Using software control
of the GC this parameter must be one in the Main and Sub Tower settings!
5.1 Software control
5.2 Compatibility
Software
GCsolution Yes Yes Yes
LabSolution Yes Yes Yes
ClassVP 7.4SP4/
EZchrom
Chromeleon Yes No No
Empower Yes No No Only single line Systems
Thermo Atlas Not released yet
GCMSsolution Yes Yes Yes
Single AOC-20i
Single AOC-20i+s
Not released yet
AOC20i Dual
with AOC20s
AOC20i Dual
without AOC20s
Comments
Chromeleon 6.8. Only GC2010 driver
Table 1: Software compatibilit y with different AOC-20i configurations .
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5.3 Software configuration AOC-20i Dual Tower with AOC-20s – example LabSolution
GC
Please notice:
Both Towers must have F97=0. If any of the used towers has set F97=1 no error message will
be shown but the Dual Tower configuration will not work properly.
Figure 11: LabSolution configuration scheme for AOC-20i Dual Tower without AOC-20s. The AOC-20d (M)
should be the front tower and connected to “injector 1 port” on the AOC-20i control board. AOC-20d(S) is the
rear side tower and connected to “injector 2 port”. AOC-20s is connected to Sampler
Figure 12: In case of AOC-20i Dual Tower with AOC-20s the syringe size can be chosen individually on both
towers. ROM versi on of AOC-20s is only read in case of AOC-20s Version 3.0 or higher. Here AOC-20s with
Firmware version 1.4 was used. This has no influences on functionality of AOC-20i Dual Tower with AOC-20s
despite of the fact that the two AO C-20i towers have Firmware Version 3.2.
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5.4 Software configuration AOC-20i Dual Tower without AOC-20s – example
LabSolution GC
In LabSolution the auto configuration does not configure the AOC-20i. Those must be
configured manually. Add AOC-20d (M) and (S) – Dual Main and Sub - to Analytical Line 1
and 2 respectively (see Fig.11).
In AOC-20d(M) the checkbox use AOC-20s must be unchecked (default it is checked). This
checkbox is used only software internally - it does not set F97 on the AOC-20i.
Figure 13: In AOC-20d (M) the checkbox Use AOC-20s” must be unchecked for Dual Tower without AOC-20s.
AOC-20i serial number is not read by LabSolution even with Firmware 3.20.
AOC-20i Dual Tower without AOC-20s allows additionally individual “Rack Types” for
both Towers. So the Main Tower (front) could be operated with long rack (Fig. 13) and the
Sub Tower (rear) with short rack (Fig. 14).
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Figure 14: In case of AOC-20i Dual Tower without AOC-20s the Rack type can be chosen individually for Main
and Sub Tower.
6.1 Appendix
6.2 Racks for AOC-20i Dual Tower with AOC-20s
On principle the standard rack for AOC-20i+s is needed two times but for Sub tower it must
be assembled differently.
Figure 15: Standard AOC-20i+s rack for 1.5ml sample vials
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Figure 16: Racks for AOC-20i+s. The default rack (identical to figure 15) is shown in the front. The rear side
one is used with of AOC-20 dual tower (Sub). The rack for the rear side tower has a mirrored configuration and
wash A is right-aligned with the rack guide. In the front tower rack the standard AOC-20i+s rack is used (1.5ml
vial hand over is left aligned)
6.3 Racks for AOC-20i Dual Tower without AOC-20s
This configuration allows different rack assembling for Main and Sub Tower. Any from the following choices
can be used individually.
6.3.1 Six sample standard rack - delivered with any AOC-20i
(P/N 221-45610-91)
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Figure 17: AOC-20i six sample rack P/N 221-45610-91
The rack consists of:
221-45608-91 Rack guide short (see (a) in drawing above)
221-32949-01 4ml vial rack (solvent wash insert; see (b) in drawing above)
221-45609-92 1.5ml vial rack 6 positions (see (c) in drawing above)
6.3.2. Twelve sample rack (P/N 221-45622-91)
Figure 18: AOC-20i twelve sample rack P/N 221-45622-91
6.3.3 Twelve sample rack modified for three solvent wash
With time the micro syringe plunger movement becomes harsh due to contamination inside
the syringe. Washing the syringe several times with solvents of different polarity makes it
smooth again. This regular maintenance of micro syringes can be extended using the three
solvent wash with solvents of different polarity.
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Figure 19: Twelve sample rack modified for three wash positions
Required are:
1x
221-45622-91 Twelve sample rack
2x
221-32949-01 4ml vial rack (solvent wash insert; large vial holder)
Please notice:
After installation of the three solvent wash option the AOC-20i cannot reach the 1.5ml vial
position twelve anymore. Means maximum vial number is eleven.
If you operate this rack and address vial number 12 in GCsolution single or batch run the
AOC-20i would not display an error massage but would inject sample from vial eleven
(checked with AOC-20i Firmware 2.1)!
To operate this rack with three solvent vials correctly on AOC-20i the Function 28 must be
set to 1 (default is 0).
This can be done in GCsolution Method parameters for AOC-20i:
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Figure 20: Settings in GCsolution and LabSolution for three solvent vials
Additionally the number of rinses must be adjusted. The wash stations are named A,B,C.
Same as shown in figure 8. Thus wash station A is at the right end of the rack in figure 3.
Figure 21: Setting of rinse steps in GCsolution and LabSolution
If the # of Rinses with solvent is set to 1 only solvent A would be used. In case of “# of
Rinses =2” solvent A and B is used. With setting 3 all solvents A,B,C are used to clean the
syringe (same for # rinses pre run).
If # of rinses is set to 4 the syringe rinse procedure would be A,B,C,A.
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6.3.4 Fifteen sample rack
If one wash position is sufficient the twelve sample rack can be extended for fifteen samples:
Figure 22: Fifteen sample rack
Required parts:
221-45622-91 12 sample rack
221-45609-91 Small vial holder (6 positions)
Please notice:
Despite that it has 18 positions for 1.5ml vials the AOC-20i can reach only 15!
This rack solution works with GCsolution V 2.21or higher, LabSolution, and AOC-20i ROM
version 2.1 or higher.
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