Sheldon SL Incubator, Lab Water Jacket CO2 Installation and user manual

WATERJACKET CO2

INCUBATORS

WITH MICROPROCESSOR CONTROL

MODELS: 3517, 3517J, & 3524

INSTALLATION AND OPERATION MANUAL

Sheldon Manufacturing Inc. P.O. Box 627 Cornelius, Oregon 97113

EMAIL: [e-mail] INTERNET: http://www.Shellab.com/~Shellab

1-800-322-4897 [phone] FAX [phone]

2

TABLE OF CONTENTS

SECTION 1.0 RECEIVING AND INSPECTION
SECTION 2.0 GRAPHIC SYMBOLS
SECTION 3.0 INSTALLATION
SECTION 4.0 CONTROL PANEL OVERVIEW
SECTION 5.0 OPERATION
SECTION 6.0 DATA LOGGER FUNCTION
SECTION 7.0 FYRITE CO CHECKING
SECTION 8.0 HEPA C02 FILTER
SECTION 9.0 MAINTENANCE
SECTION 10.0 TROUBLESHOOTING
SECTION 11.0 PARTS LIST
UNIT SPECIFICATIONS
SCHEMATICS

REV 04/08

4861607

These units are CO Water Jacketed incubators for professional, industrial or educational
use where the preparation or testing of materials is done at approximately atmospheric
pressure and no flammable, volatile or combustible materials are being heated. These
units are not intended for hazardous or household locations or use.

3

INTRODUCTION

Thank you for choosing a water jacket incubator. These units are not intended for use at
hazardous or household locations.

Before you use the unit, read this entire manual carefully to understand how to install,
operate, and maintain the unit in a safe manner. Your satisfaction with the unit will be
maximized as you read about its safety and operational features.

Keep this manual on-hand so it can be used by all operators of the unit. Be sure all
operators of the unit are given appropriate training before you put the unit in service.

Note: Use the unit only in the way described in this manual. Failure to follow the

guidelines and instructions in this manual may be dangerous and illegal.

General Safety Considerations

Your incubator and its recommended accessories have been designed and tested to
meet strict safety requirements.

For continued safe operation of your incubator, always follow basic safety precautions
including:

 Read this entire manual before using the incubator.
 Be sure you follow any city, county, or other ordinances in your area regarding the use

of this unit.

 Use only approved accessories. Do not modify system components. Any alterations or

modifications to your incubator may be dangerous and will void your warranty.

 Always plug the unit’s power cord into a grounded electrical outlet that conforms to

national and local electrical codes. If the unit is not grounded, parts such as knobs
and controls may conduct electricity and cause serious injury.

 Do not connect the unit to a power source of any other voltage or frequency beyond

the range stated on the power rating on the dataplate inside the door of the unit.

 Do not modify the power cord provided with the unit. If the plug does not fit an outlet,

have a proper outlet installed by a qualified electrician.

 Avoid damaging the power cord. Do not bend it excessively, step on it, place heavy

objects on it. A damaged cord can easily become a shock or fire hazard. Never use a
power cord after it has become damaged.

 Do not position the equipment in such a manner as to make it difficult to disconnect

power cord or coupler.

 Do not attempt to move the unit while in operation or before the unit has been allowed

to cool.

4

Section

1

RECEIVING AND INSPECTION

IMPORTANT: READ THIS INSTRUCTION MANUAL IMMEDIATELY!

Your satisfaction and safety require a complete understanding of this unit, including
its proper function and operational characteristics. Be sure operators are given
adequate training before attempting to put the unit in service. NOTE: This equipment
must be used only for its intended application; any alterations or modifications will
void your warranty.

1.1 Inspection: The carrier, when accepting shipment, also accepts responsibility for
safe delivery and is liable for loss or damage claims. On delivery, inspect for visible
exterior damage, note and describe on the freight bill any damage found and enter
your claim on the form supplied by the carrier.

1.2 Inspect for concealed loss or damage on the unit itself, both interior and exterior. If
any, the carrier will arrange for official inspection to substantiate your claim. Save the
shipping crate until you are sure the unit has been delivered in good condition.

1.3 Return Shipment: If for any reason you must return the unit, contact your customer
service representative for authorization and supply nameplate data. For information
on where to contact Customer Service please see the manual cover.

1.4 Accessories: Make sure that all of the equipment indicated on the packing slip is
included with the unit. Carefully check all packaging before discarding. Each
chamber comes equipped with 3 shelves, 4 shelf standards, 1 humidity pan and a
power cord. Each chamber of the 3517J comes equipped with 5 shelves, 4 shelf
standards, 1 humidity pan and a power cord. All units are shipped with 4 leveling
feet and supply hose kit.

5

GRAPHIC SYMBOLS

Section

2

Your incubator has been provided with a display of graphic symbols which is designed to
help in identifying the use and function of the available user adjustable components.

2.1 Indicates that you should consult your manual for further description or

discussion of a control or user item.

2.2 Indicates "Temperature".

2.3 Indicates "Over-temperature safety".

2.4 C Indicates "Degrees Centigrade".

2.5 CO

2.6 Indicates "Gas" (CO2 for this unit).

2.7 Indicates "AC Power ".

2.8 Indicates "Water Jacket Low".

2.9 Indicates "ON/I" and "OFF/O".

2.10 Indicates "Relative Humidity".

2.11 %RH Indicates "Percent Relative Humidity".

2.12 Indicates "Potential Shock Hazard" behind this protective partition.

2.13 Indicates "Protective Earth Ground".

Indicates "Carbon Dioxide".

2

2.14 Indicates “Unit should be recycled” (Not disposed of in land-fill)

6

INSTALLATION

Section

3

Local city, county, or other ordinances may govern the use of this equipment. If you have any
questions about local requirements, please contact the appropriate local agency. Installation may be
performed by the end user.

Under normal circumstances this unit is intended for use indoors, at room temperatures between 5
and 40C, at no greater than 80% Relative Humidity (at 25) and with a supply voltage that does not
vary by more than 10%. The unit’s technical and environmental specifications includes: altitude not
exceeding 2000 m operating and Pollution Degree of 2. Customer service should be contacted for
operating conditions outside of these limits.

CAUTION: Make sure that the incubator is located in its intended position and level before
filling the water jacket. Directions for filling the jacket are in Section 5.2.

3.1 Power Source: The unit power requirements are listed on the data plate. PLUG THE UNIT

INTO A PROPERLY GROUNDED AND RATED RECEPTACLE OF THE CORRECT
STYLE. A separate circuit is recommended for this unit to prevent loss of product due to
overloading or circuit failures caused by other equipment. The power supply must be
properly grounded (earthed) and correctly sized to match the unit nameplate rating. The
supply voltage must match the nameplate voltage within +/- 10%. The US market units
are designed for 110 volts @ 60 Hz, 5 Amps. The EU market units are designed for 220
volts @ 50 Hz, 3.5 Amps. If supplied with a detachable cord set, plug the female end into
the inlet on the unit and the male plug into the supply. Assure that units requiring fuses
have them installed. The fuse is a T type, 10 amp for 110 vol units, 6.3 amp for 220 volt
units. This fuse may be at the inlet or a part of the cord set male plug. The unit must be
located to easily allow for it to be unplugged or disconnected.

3.2 Location: When selecting a site for the unit, consider conditions which may affect
performance, such as heat from steam radiators, ovens, autoclaves, etc. Avoid direct sun,
fast-moving air currents, heating/cooling ducts, and high-traffic areas. To ensure air
circulation around the unit, allow a minimum of 10cm of clearance between the incubator
and surrounding walls, or obstructions to free air flow.

3.3 Lifting / Handling: These units are heavy and care should be taken to use appropriate
lifting devices that are sufficiently rated for these loads. Units should only be lifted from their
bottom surfaces. Doors, handles and knobs are not adequate for lifting or stabilization. The
unit should be completely restrained from tipping during lifting or transport. All moving parts,
such as shelves and trays should be removed and doors need to be positively locked in the
closed position during transfer to prevent shifting and damage.

3.4 Leveling: The unit must sit level and solidly. Leveling feet are supplied and must be
installed in the four holes in the bottom corners of the unit. With the feet installed and the
unit standing upright, each foot can be raised by turning it in a counterclockwise direction.
Adjust the foot at each corner until the unit stands level and solid without rocking. If the unit
must be moved, drain all of the water from the unit and turn the leveling feet in (clockwise)
all the way to prevent damage while moving.

7

3.5 Cleaning: The unit chamber should be cleaned and disinfected prior to use. The
operating conditions and appropriate protocol will determine the correct procedure for
decontamination. A typical decontamination procedure that is adequate for many
situations has been described below. As well, certain steps are listed that will help reduce
the likelihood of contamination and the necessity of decontamination. Whatever process
is appropriate, it needs to be done on a regularly scheduled basis. Depending on usage
and protocol, this may be monthly, quarterly or otherwise. Regardless of the
decontamination procedure used, certain precautions will need to be taken:

A. Always disconnect the unit from the electrical service when cleaning. Assure all

volatile or flammable cleaners are evaporated and dry before reconnecting the unit
to the power supply.

B. Special care should be taken when cleaning around sensing heads to prevent

damage.

C. If you use chlorine-based bleaches or abrasive cleaners this will modify the

stainless steel interior finish. DO NOT USE hard tools such as metal wire brushes
or steel wool. Use non-abrasive cleaners and soft tools such as plastic brushes.

D. In the event hazardous material is spilled onto or into the equipment, appropriate

decontamination must be carried out. If there is any doubt about the compatibility
of decontamination or cleaning agents with parts of the equipment or with material
contained, please contact the manufacturer or his agent. No decontamination or
cleaning agents should be used which could cause a hazard as a result of a
reaction with parts of the equipment or with the material contained in it.

TYPICAL DECONTAMINATION PROCEDURE

1. Remove the humidity pan every week and autoclave, or wash with soap and water

then disinfect with 70% alcohol solution. Replace in the incubator with fresh,
DISTILLED water.

2. Flush the sample port tubing with 70% alcohol solution. Replace any lines that

have a green color.

3. Remove the door gaskets, clean and disinfect. Clean and disinfect all mounting

grooves for the door gaskets.

4. Remove all shelves, shelf supports, shelf standards and shields. Autoclave, or

wash and disinfect as described in item 1.

5. Wash and disinfect all interior surfaces.

6. Give special attention to cleaning and disinfecting all access ports, air bleeds,

shaft holes, electrical pass-through and any other passages into the chamber.

7. Replace all air and CO2 filters every six months or when noticeably dirty on the

upstream side. CO2 filters are located in the shadow box just behind the GAS IN
fitting and in line with the CO2 tubing kit. Units with flow meters require operator­supplied air pumps. These pumps should be checked for proper air filtering.

OPERATION FOR MINIMIZING CONTAMINATION

1. Keep the outside of the incubator, including the air in the laboratory, as clean as

possible. This is particularly important for units placed directly on the floor. Do
not place incubators near doors, air vents or other areas of high air movement or
traffic.

8

2. The floor around the unit needs to be clean. Units that are placed on the floor

should be mounted higher – typically on a caster platform – for ease of moving the
unit during cleaning and access to the back of the unit.

3. Minimize the number of times access is made to the chamber during normal

operation.

4. Do not depend on the use of antibiotics to maintain uncontaminated conditions, as

this is an inadequate technique for sterilization. Preferably use aseptic techniques
as described above for maintaining sterile conditions in the incubator.

3.6 Shelves and Interior Parts: Shelving, shelf supports and a humidity pan are supplied with
each unit. See Figure 1, for proper placement of these accessories.

Figure 1

9

CONTROL PANEL OVERVIEW (See Figure 2)

Section

4

All controls are located on the front panel. Units with a detachable cord have a fused inlet
located at the top rear of the control panel. This inlet has a recessed male plug, fuse and
an EMI filtering system designed to filter out electrical interference. This inlet also prevents
any internally generated interference from feeding out to the power grid.

4.1 Power Switch: The I/O (ON/OFF) switch controls all of the power for the incubator
and must be in the I/ON position before any systems are operational. Both
temperature and CO2 displays will illuminate when the power switch is in the ON
position.

4.2 Water Low Light: This pilot lamp is on whenever the internal water jacket float
switch has been tripped to the closed position. When the water drops low enough
the float switch closes the circuit turning on the indicator light.

4.3 Over Temperature Light: This pilot lamp is on whenever the Over Temperature
Safety thermostat has been activated and taken control of the element. During
normal operating conditions this indicator should never be on.

4.4 Main Temperature Control: This controller is marked in C and indicates the
actual temperature within the chamber to .1C. The UP/DOWN buttons are used for
imputing the set point, calibrating the display, and muting or unmuting the audible
alarm. The HIGH and LOW alarm indicators will light whenever there is an alarm
condition associated with the temperature within the chamber. The MUTE indicator
will light whenever the audible alarm has been deactivated. Some alarm conditions
have a delay programmed in.

4.5 Heating Light: This pilot lamp is on whenever the Temperature Controller is
activating the heating element.

4.6 CO2 Control on Water Jacket Incubators: The controller is marked “CO2 %”
and has a resolution of .1 %. It has an up and down arrow that is used for
imputing CO2 set points, calibrating the CO2 concentration level, and muting or
unmuting the audible alarm. It also has three led indicating lights for alarm
conditions, one for high CO2 levels, one for low CO2 levels, and one for alarm
mute. On some older model the up and down arrows can also be used to reset the
control integrator value. On newer Models it isn’t necessary, but can be done also.

4.7 Injection Light: This pilot lamp is on whenever the CO2 controller is injecting CO2
into the chamber.