Secomam UVIKON XL, UVIKON XS Operating Manual

UVIKON XL

UVIKON XS

Operating manual

Ref. 0M7717B

SUMMARY

1. REMARKS..................................................................................................................................................... 3

2. WARRANTY.................................................................................................................................................5

3. INFORMATION ...........................................................................................................................................7

4. PRECAUTION OF USE............................................................................................................................... 9

4.1. GENERAL WARNINGS ........................................................................................................................9

4.2. DESCRIPTION OF SYMBOLS............................................................................................................10

4.3. QUALITY, RELIABILITY AND SAFETY..........................................................................................10

5. INSTRUMENT DESCRIPTION ............................................................................................................... 13

5.1. GENERAL POINTS..............................................................................................................................13

5.2. SPECIFICATIONS ...............................................................................................................................14

5.3. SYSTEM CONCEPT ............................................................................................................................ 15

5.3.1. Display...........................................................................................................................................15

5.3.2. Electronics.....................................................................................................................................15

5.3.3. Optics.............................................................................................................................................15

6. INSTALLATION ........................................................................................................................................17

6.1. UNPACKING AND INSTRUMENT SETUP....................................................................................... 17

6.1.1. Unpacking......................................................................................................................................17

6.1.2. Checking the serial number of the instrument............................................................................... 18

6.2. INSTALLING THE INSTRUMENT ....................................................................................................19

6.2.1. Environmental conditions..............................................................................................................19

6.3. INITIAL INSTALLATION...................................................................................................................20

6.3.1. Connexion of the power supply......................................................................................................20

6.3.2. Connection to the Personal Computer...........................................................................................20

6.3.2.1. PC minimum requirements.........................................................................................................................20

6.3.3. Software Installation, LabPower JUNIOR ....................................................................................20

7. OPERATING INSTRUCTIONS................................................................................................................ 21

8. MAINTENANCE ........................................................................................................................................23

8.1. DECONTAMINATION........................................................................................................................23

8.1.1. Requirements.................................................................................................................................23

8.1.2. Risks...............................................................................................................................................23

8.2. CLEANING THE INSTRUMENT........................................................................................................24

9. OPTIONS AND ACCESSORIES .............................................................................................................. 25

9.1. INTERNAL ACCESSORIES................................................................................................................25

9.2. EXTERNAL ACCESSORIES............................................................................................................... 27

9.3. ACCESSORY CONFIGURATIONS....................................................................................................27

1.

REMARKS

The Automatic aspiration system kit is manufactured by AQUALABO ANALYSE.
The information contained in this document may be the object of patents or patent applications by

AQUALABO ANALYSE. The possession of this document does not confer any license to such
patents.

The following names are AQUALABO ANALYSE’s registered trademarks throughout the world.

SECOMAM
UVIKON XL, UVIKON XS

This manual is updated periodically. The updates are included in the new editions.
All information supplied in this edition of the manual may be amended before the products described

herein are available.

All reproduction rights reserved.

2.

WARRANTY

The new equipment and material sold by AQUALABO ANALYSE is guaranteed against any
manufacturing defects for one year (unless otherwise stated by AQUALABO ANALYSE) with effect:

− From the technical acceptance of the equipment in the factory by the buyer or his designee,

− or failing this :

* For Metropolitan France: from the date on the delivery note.
* For other destinations: from the date of factory shipment certified by air waybill,

consignment note or bill of lading.

The AQUALABO ANALYSE company guarantee applies exclusively to defectiveness arising from a
design fault or from a concealed defect. It is strictly limited to the free dispatching of replacement parts
(except for consumable items) or to the repairing of the equipment in our workshops within a deadline
of 10 working days (shipping delay not included).

By express agreement, the following are strictly excluded from our guarantee:

− All damages, notably for staff costs, loss of earnings, business trouble, etc

− Any breakdown due to an incorrect use of the equipment (non adapted mains, fall, attempt at

transformation, etc) or to a lack of maintenance by the user or to poor storage conditions.

− Any breakdown due to the use of parts not supplied by AQUALABO ANALYSE, on AQUALABO
ANALYSE equipment

− Any breakdown due to the transporting of the equipment in packaging which is not its original
packaging

− The lamps, the cells and generally any item which appears in the "accessories" section on the
price list.

Our customers are kindly asked to apply for our consent before returning any instrument for repair. No
return of materials may be accepted without the prior written consent of our Servicing after Sales
Management which will precise the terms of such return.
If the above consent is given, articles shall be returned in their original packaging on a prepaid basis to
the following address:

AQUALABO ANALYSE – USINE SECOMAM - 91 avenue des Pins d’Alep - 30100 ALES - France

We reserve the right to reship all instruments received collect failing such consent.
Whatever method and conditions of transport are chosen for the shipment of the equipment to be

repaired under guarantee, in the original packaging, the corresponding costs and the insurance costs
will be payable by the customer.

Any damage connected to the return transport of the equipment falls within the framework of the
guarantee on the express condition that the customer has sent his complaint within forty-eight hours
by registered letter with acknowledgement of receipt to the carrier. A copy of the letter should be sent
to AQUALABO ANALYSE.

For equipment with a guaranty card, this is only applicable if the card delivered with the equipment is
returned to AQUALABO ANALYSE duly completed.

UVIKON XL/XS

- 6 -

SOFTWARE GUARANTEE

The software is guaranteed by the designer or the distributor of the software under the conditions
specified in the literature accompanying the aforementioned software packages.
Under no circumstances whatsoever will AQUALABO ANALYSE supply any type of guarantee for
software packages.
By express agreement, all damages, notably for staff costs, lost of earnings; business trouble, etc are
strictly excluded from our guarantee.
The customer is informed that the software cannot be guaranteed exempt from defects or bugs.

TRADE SECRET AND PROPERTY RIGHTS

This document is protected by a AQUALABO ANALYSE copyright (c) 2003 and the copying rights are
explicitly reserved. The software supplied with the equipment or referenced contains trade secrets and
confidential information which are AQUALABO ANALYSE's property. It is legally protected by the
international copyright (c) laws.

AQUALABO ANALYSE grants a license to use its software to the user. This may not be disclosed,
used or duplicated with the intention to save it, without AQUALABO ANALYSE's written permission.
The beneficiary must attach a copy of this document to all authorized partial or total reproductions.

3.

INFORMATION

The AQUALABO ANALYSE - SECOMAM equipment has been designed, manufactured, tested and
inspected according to the ISO 9001 standards (Version 2000).

If the unit is not immediately installed, it should be stored in a dry and clean area. The storage
temperature should be between 10 and 35°C.

AQUALABO ANALYSE – SECOMAM equipment is carefully inspected before it is packed. As soon as
you receive your equipment, check the condition of the packaging and if you notice any problems,
notify your carrier within 48 hours. Then consult the packing list and check that everything is in order.
Finally, if you discover that something is missing, or if the goods are damaged immediately notify :

AQUALABO ANALYSE

USINE SECOMAM

91, Avenue des Pins d’Alep

30100 ALES

FRANCE
Tél. : [phone]
Fax : [phone]

E-mail : [e-mail]

Web: www.secomam.fr

4.

PRECAUTION OF USE

The user should, before using this equipment, have read and understood this operating
manual, particularly the general warnings given below

4.1. GENERAL WARNINGS

Always disconnect the mains plug before starting any work inside the
instrument

Any maintenance operation inside the instrument, including lamp
replacement, must be carried out by a service engineer or trained
personnel authorised by SECOMAM. The user is permitted
access only to the inside of the sample compartment

Keep the sample compartment clean
The Deuterium lamp used in this unit emits UV radiation

Install the instrument in a ventilated area because it is likely to generate
ozone, which, beyond the limits below, can harm health.

Exposure average value = 100 ppb
Exposure limits value = 200 ppb

It is important that where the equipment and accessories can come into
contact with human or animal tissues or fluids (particularly blood),
they should always be regarded as contaminated and potentially
hazardous (See Section 5 for decontamination)

UVIKON XL/XS

- 10 -

4.2. DESCRIPTION OF SYMBOLS

Caution (refer to enclosed documents). In the lamp
compartment, it indicates the presence of high temperature and
ozone generated by the Deuterium lamp.

Power on

Power off

AC (alternating current)

DC (direct current)

Protective earth conductor terminal

Caution, risk of electric shock

Caution, hot surface

4.3. QUALITY, RELIABILITY AND SAFETY

This equipment has been designed with emphasis on QUALITY, RELIABILITY and
SAFETY. AQUALABO ANALYSE will accept responsibility for these aspects only if the
following conditions are met:

a) All electrical installations in the room or building in which the equipment is to

be used must comply with regulations specified in the country where the
equipment is to be used.

b) The equipment must be used in accordance with the instructions for use

provided by AQUALABO ANALYSE.

c) All modifications and repairs to the equipment must be carried out by

authorised AQUALABO ANALYSE Personnel, their Agents or authorised
Technicians.

d) Modifications must not be carried out unless they conform to approve

Engineering Service Information issued according to the appropriate
AQUALABO ANALYSE procedure.

e) The equipment installation must be carried out in accordance with local

requirements for responsibility and warranty.

NOTE: Routine tests
Routine test are performed on 100% of the produced equipment according to

the requirements specified in Annex K of the Safety Standard EN 61010­ 1+A2: + Dev. UL/CSA

5.

INSTRUMENT DESCRIPTION

5.1. GENERAL POINTS

AQUALABO ANALYSE - SECOMAM is proud to introduce the newest series of UVIKON
ultraviolet-visible spectrophotometers: the UVIKON Simplicity Concept with DSP
Technology.
The Advanced Digital Signal Processing Technology inside the UVIKON combines its
high-performance true double beam optical design with rapid scanning and powerful yet
intuitive software.

The UVIKON is engineered to make it quick and easy for all to use – regardless of the
analytical method – without sacrificing capabilities.

The UVIKON Xx spectrophotometer is a double beam spectrophotometer, which employs
state of the art technology for collection, presentation, storage and retrieval of analysis
data.

The UVIKON Xx is completely controlled via the RS-232 interface by an external personal
computer.

The UVIKON Xx software, provided with the spectrophotometer, runs under Windows NT
operating software.

Light sources Deuterium and tungsten-halogen lamps
Monochromator High energy low stray light diffraction grating with 1300

lines/mm, 175mm focal length.

Detector R955 Photomultiplier (Uvikon XL)

Photodiode (Uvikon XS)

Dimensions W 680 mm x D 565 mm x H 275 mm
Weight 35 Kg net
Mains Voltage

100 - 240VAC ±10%

Power 200VA
Frequency 50 - 60 Hz

UVIKON XL/XS

- 14 -

5.2. SPECIFICATIONS

UVIKON Xx is a double beam high performance spectrophotometer with the following
specifications:

UVIKON XL UVIKON XS
Wavelength range 180-900 nm 190-1100 nm
Wavelength step 0.05-10 nm 0.05-10 nm
Spectral bandwidth 0.2 - 0.5 – 1 – 2 - 4 nm;

0.5– 1–2- 4 (reduced height)

1.8 fixed

Scan speed 10-5000 nm/min 10-2000 nm/min
Transfer speed 7000 nm/min 5000 nm/min
Wavelength accuracy

Holmium peaks,
10nm/min, 0.05nm step

± 0.25 nm ± 0.3 nm

Wavelength precision
Holmium Oxide filter shoulder

± 0.025 nm ± 0.03 nm

Baseline flatness
200-800nm, 2nm, 200nm/min

≤ ±1mAbs

≤ ±1mAbs

Stray light
NaI, 220nm, 1nm Bw,

≤ 0.015 %T ≤ 0.03 %T

Linearity
1sec, 2nm, r² = 0.999 (250nm)

0-3.3 Abs 0-3.0 Abs

Photometric range ± 5 Abs ± 3.5 Abs
Photometric accuracy

1Abs NIST, 1sec, 590nm

± 3 mAbs ± 3 mAbs

Photometric precision
1Abs NIST, 1sec, 590nm

± 0.5 mAbs ± 0.5 mAbs

Noise RMS
0Abs, 500-600nm, 2nm, 1sec

40 µAbs 30 µAbs

Drift
580nm, 2nm, 1 sec - warm-up 2 hour

0.1 mAbs/h 0.1 mAbs/h

Response time 0.02-5 sec 0.02-5 sec

5.3. SYSTEM CONCEPT

UVIKON Xx is a double beam high performance spectrophotometer controlled from an
external PC running under MS Windows NT.

The communication between PC and spectrophotometer is via a standard RS232
connection
Minimum system requirements to run the UVIKON SW see section 3; as minimum a 17
inch monitor is suggested.

5.3.1. Display

Below is the layout of the UVIKON XL status display with the description of the
symbols.

SYSTEM FAILURE: Red light indicates a system error
MEASURE: Green light shows that UVIKON XL is measuring
HALOGEN LAMP: Green lamp indicates halogen lamp on
DEUTERIUM LAMP: Green light indicates Deuterium lamp on or igniting
POWER ON Green light indicates system power is on

5.3.2. Electronics

Data processing is executed using single channel analogy electronics with 20 bit
A/D converter and DSP processor. High speed microcontrollers control the
photometer drive and the communication with PC. Firmware is stored in Flash
memory and can be upgraded by PC download.

5.3.3. Optics

The basic optical concept is as follows:
Light is provided by D2 and Halogen lamps with a switching mirror and single

monochromator. The beam is split by a rotating chopper wheel which reflects
light via a set of mirrors onto either a photomultiplier detector (XL) or photodiode
(XS).

System failure

Measure

Halogen lamp

Deuterium lamp
Power on

UVIKON XL/XS

- 16 -

6.

INSTALLATION

6.1. UNPACKING AND INSTRUMENT SETUP

6.1.1. Unpacking

The UVIKON Xx is contained in one single packing case. Open the case, lift the
instrument out of the box and place it on a table.

The instrument is heavy, do not lift alone. Have at least one other
person help whenever lifting or moving the instrument.
Most of the accessories are contained in a separate enclosure.
Please make sure that you have removed these before storing the
box, do not discard.

Allow suitable space for connecting and disconnecting the power
cable and switching the instrument on/off.

Check the consignment against the shipping list. If any parts are
damaged, please inform your local AQUALABO ANALYSE representative
immediately.

UVIKON XL/XS

- 18 -

NOTE: Use only the original packing case to return the unit to the factory for

any reason.

LAB POWER JUNIOR Set up program
APPLICATIONS Set up program
LAB POWER JUNIOR Software User Manual
VALIDATION Software User Manual
DNA/RNA Purity Check - Software User Manual

6.1.2. Checking the serial number of the instrument

The plate showing the serial number is mounted to the rear right hand side of the
instrument. This number must be identical with the serial number on the delivery
note. Should this not be the case please inform the local AQUALABO ANALYSE
representative immediately.

RS232 - PC connection

Mains plug

6.2. INSTALLING THE INSTRUMENT

6.2.1. Environmental conditions

Performance and safety of the equipment are guaranteed only if it is operated
under the following environmental conditions:

The instrument is designed for internal use and altitude below 2000 metres
Installation category II with smaller transient over-voltages (IEC 664)
Pollution degree 2 (CEI EN 61001-1)
Room temperature between 15°C (59°F) and 35°C (95°F)
In locations where the relative humidity is lower than 80%

The UVIKON Xx contains many sensitive optical components, to obtain optimum
performance avoid using this instruments under the following conditions:

In locations subjected to dust or vapours of solvents and acids
In locations prone to vibrations
On an unstable or uneven surface
Near appliances generating strong magnetic fields

UVIKON XL/XS

- 20 -

6.3. INITIAL INSTALLATION

It is most important that there is sufficient free space around the instrument for
adequate ventilation (10cm).

6.3.1. Connexion of the power supply

The power is supplied via the enclosed plug-in power pack. The power pack supplies the photometer
with low voltage (12 VDC).

Warning
The line voltage of the usage location must fulfil the specifications stated on the power pack.

Always use the supplied 12 V original power pack only. The outlet must have a protective earth
connection.

1) Connect the miniplug of the power pack to the main plug of the spectrophotometer.

2) Connect the power pack to an easily accessible power socket.

The main switch is located in the lower left side of the equipment

6.3.2. Connection to the Personal Computer

Use PC COM1 for the connection of the spectrophotometer serial cable and
COM2 for external UVIKON XL accessories (see section 6). Connect the serial
cable between UVIKON Xx 9 poles DIN connector located on the rear right hand
side and PC serial port.

Baud rate must be set to 38400 bit/sec
NOTE: Use only equipment, e.g. PC, monitor, keyboard etc., which

comply with the standard EN60950
Refer to the specific documentation for hardware connection and software

installation

6.3.2.1. PC minimum requirements

CPU: 300 MHz
RAM: 64 MB
Hard Disk 2 GB
CD ROM drive
Operating system Windows NT 4.0 + Service pack 4 only

6.3.3. Software Installation, LabPower JUNIOR

1. Insert the CD-ROM (70/91-91640)

2. Select Run from the Start menu

3. Select the Set up program from the CD_ROM Lab Power Junior directory

4. Press OK to start the Set up program

5. Follow the on-screen instructions

7.

OPERATING INSTRUCTIONS

Verify the proper connection of the spectrophotometer and PC via RS232 cable serial
cable, part number 70/93-90307.

Use PC COM1 for the connection of the spectrophotometer serial cable and COM2 for
external UVIKON Xx accessories (see section 6).

Switch on the spectrophotometer and the PC.
Launch Windows NT and press the corresponding LAB POWER icon in order to load the

UVIKON spectrophotometer software.
If the communication is not active the sequence of messages will finish with:

Connect: Service terminated with error

In case of this message check that:

• the spectrophotometer is on;

• for correct connection between spectrophotometer and PC.

Also verify in the UVIKON SW communication window that:

• serial port = COM1

• baud rate = 38400

8.

MAINTENANCE

WARNING
Any maintenance operation inside the instrument, including the lamp

replacement, must be carried out by a specialised service engineer
authorised by AQUALABO ANALYSE

The user is permitted access only to the inside of the sample compartment.

Your UVIKON Xx has been constructed using precision components and will require very little
daily maintenance other than keeping the unit clean.

8.1. DECONTAMINATION

8.1.1. Requirements

Due to the nature and seriousness of diseases such as the Human
Immunodeficiency Virus (HIV) and Hepatitis B, it is important that, where
equipment and accessories can come into contact with human or animal tissues
or fluids (particularly blood), they should always be regarded as contaminated
and potentially hazardous.

Contaminated equipment and accessories must be decontaminated using the
hospital decontamination and disinfecting procedures appropriate for the device.

Decontamination must be carried out by properly trained staff.
If you have any doubt regarding contamination or decontamination, consult your

local infection control officer.

8.1.2. Risks

All human or animal tissues or fluids in the hospital and laboratory environment
are capable of transmitting infection. Therefore, special precautions must be
taken in hospitals and laboratories that deal with patients who have highly
infectious disease.

Infection can occur by the following routes:
Through broken skin;
Needles or other sharp objects;
Contamination of cuts, abrasions or burns;
Ingestion (placing a contaminated object in
or near the mouth, e.g. pen, finger);
Direct contact with mucous membranes (e.g. eyes);
Inhalation of contaminated dust or aerosols.

UVIKON XL/XS

- 24 -

8.2. CLEANING THE INSTRUMENT

The sample compartment windows are not accessible from inside the sample
compartment. Keep the sample compartment clean.

To clean the instrument and the sample compartment use only a dry or
water/neutral detergent wet cloth, do not use organic solvents or abrasive agents.

Do not use solvents, e.g. acetone, to clean the instrument

.

9.

OPTIONS AND ACCESSORIES

9.1. INTERNAL ACCESSORIES

Accessories driven by the spectrophotometer to be connected to the mechanical and
electrical connections in the sample compartment.

70/91-91672 The integrating sphere is designed for measuring the transmittance of

very turbid or colloidal solutions either in liquid or solid form, highly light
scattering solid samples, the colour of solid samples and the diffuse or
specular reflectance of solid samples.

70/91-00604 QuickLock cell holder for 10 mm cells, thermostatable. Supplied as

standard with the instrument.
70/91-00606 QuickLock cell holder for 10/20 mm cells, thermostatable.
70/91-00601 QuickLock long path rectangular cell holder 10 to 100 mm path length.
70/91-00603 QuickLock cylindrical cell holder
70/91-00580 Magnetic stirrer accessory, for stirring of reference and sample. The

speed is variable. Supplied with 5 Teflon-coated bars (8x3 mm). To be

used with 70/91-00604.
70/91-00616 Teflon-coated magnetic bars, set of 5 bars (8x3 mm).
70/91-91671 The automatic sipper system is based on a peristaltic pump. Different tube

materials can be used.

Includes thermostatting fluid connection block and tube set.

It can be directly connected to the Thermopack or an external circulating

water bath.
70/91-00608 Temperature sensor for cell content. Miniaturised sensor with inert glass

body to measure the temperature within the cell. A special protective

holder is supplied, which can be fixed in the sample compartment.
70/91-00607 Temperature sensor for holder. Measures the temperature of the cell

holders.
70/91-00586 QuickLock automatic 6+6 cell changer. Two independently driven cell

holders allow the measurement of up to 10 kinetic samples in one run.

Thermostattable cell holders and unique climate chamber for best

temperature distribution are included.

Requires connection to an external circulating water bath, includes

thermostatting fluid connection block.
70/91-00627

Standard flow cell 160 µµL. This cell is used together with the Quick Lock

automatic sipper for 10 mm cells. The flow cell has an internal volume of

160 µL. Other flow cells can be used.

UVIKON XL/XS

- 26 -

70/K-0710-Q

Flow cell, 80µµL, 10 mm path
70/K-2210-Q2 Open microcells, 4 mm inner width, 10 mm path, set of 2
70/K-2410-Q2 Stoppered microcells, 4 mm inner width, 10 mm path, set of 2
70/K-2710-Q2 Open microcells, 2 mm inner width, 10 mm path, set of 2
70/K-2810-Q2 Stoppered microcells, 2 mm inner width, 10 mm path, set of 2
70/K-2910-Q2 Open rectangular cells, 10 mm path, set of 2
70/K-2910-Q4 Open rectangular cells, 10 mm path, set of 4
70/K-3010-Q2 Stoppered rectangular cells, 10 mm path, set of 2
70/K-3010-Q4 Stoppered rectangular cells, 10 mm path, set of 4

The following picture shows the connectors of the internal accessories in the sample compartment.

Cell Changer - Sipper

Temperature sensor

Stirrer

RS232 optional

9.2. EXTERNAL ACCESSORIES

Accessories with an external controller to be driven by the PC via RS232 COM2.

70/91-91488 The thermopack accessory can be used to control the temperature of all

thermostatable cell holders and of the cell changer. The fluid circulated is

thermostated via Peltier effect.

70/91-91637

The THERMOSYSTEM consists of a quick lock automatic 6+6 Cell

Changer with Peltier effect thermostatting system and magnetic stirring

capability.

The Thermo System must be connected to an external liquid circuit for

cooling of the Peltier elements.

9.3. ACCESSORY CONFIGURATIONS

QuickLock cell holder for 10 mm cells

The drawing overleaf shows all available options, which can be used together with the
standard cell holder. The holder is shown with the tube set (70/91-00622) and the
thermostatting fluid connection block (70/91-00610), which are used for external
temperature control.

Temperature sensor for holder (70/91-00607)

This sensor measures the temperature of the sample or reference cell holder. The sensor
fits into a threaded hole in the cell holder. This sensor can be used together with the
magnetic stirrer (70/91-00580).

Temperature sensor for cell content (70/91-00608)

This sensor measures the sample temperature in a standard 10 mm cell. This sensor
can be used together with the magnetic stirrer (70/91-00580).

Magnetic stirrer accessory (70/91-00580)

The magnetic stirrer accessory fits into the base of the cell holder. It can be used together
with any external temperature control system, the temperature sensor for cell content
(70/91-00608) and the temperature sensor for holder (70/91-00607).

Water bath and thermopack.

We recommend use of the thermostatting fluid connection block and the tube set (70/91-

00622) should an external water bath be employed for temperature control of the cell
holder.

QuickLock automatic 6+6 cell changer
Temperature sensor for cell content (70/91-00608)

This sensor measures the temperature of a sample contained in a standard 10 mm cell in
any one of the 12 sample positions of the cell changer. It cannot be used for external
temperature control but is very useful for monitoring the actual sample temperature. The
tubes of an external circulating water bath can be directly connected to the fluid
connection block of the cell changer.

UVIKON XL/XS

- 28 -

For more information, go to www.aqualabo-group.com

© 2015 AQUALABO-GROUP - All rights reserved

AQUALABO
90 rue du Professeur P. Milliez
94506 Champigny-sur-Marne - FRANCE
Tel.: [phone]- Fax : [phone]
[e-mail] - www.aqualabo.fr