Secomam Basic User manual

BASIC

70VB0358
70VB0370

User’s manual

Ref. 0NVBAU

Manual version: 1.19

SECOMAM, a NOVA ANANLYTICS Company,

91, Avenue des Pins d’Alep
30319 ALES FRANCE
Tel: [phone]
Fax: [phone]
E-mail: [e-mail]
URL: www.secomam.fr

SYSTEME QUALITE CERTIFIE

BASIC

SUMMARY

1 REMARKS......................................................................................................................................................... 5

2 WARRANTY.....................................................................................................................................................7

3 INFORMATION................................................................................................................................................ 9

4 PRECAUTIONS OF USE............................................................................................................................... 11

5 PRELUDE........................................................................................................................................................ 13

6 DESCRIPTION................................................................................................................................................ 15

6.1 GENERAL OVERVIEW 15

6.2 SCREEN AND KEYBOARD 15

6.3 DISPLAY DESIGN 17

7 SET-UP............................................................................................................................................................. 19

7.1 GENERAL SET-UP 19

7.2 LANGUAGE SET-UP 20

7.3 DATE AND CLOCK SET-UP 21

7.4 PUMP SET-UP 21

7.5 PRINTER SET-UP 23

7.6 TRACEABILITY SET-UP 24

7.6.1 Downloading to a PC : F1 « BASIC –PC » 25

7.6.2 Downloading to a printer : F2 « BASIC-PRINTER » 26

7.6.3 KINETICAL REPORT 28

7.7 QUALITY CONTROL SET-UP 28

8 PROGRAMMATION ..................................................................................................................................... 33

8.1 PROGRAM KEYS 33

8.2 RECOMMANDATION FOR DAILY ANALYSIS 41

8.3 ERROR MESSAGES 43

8.4 SAMPLE NUMBERING 45

9 MAINTENANCE............................................................................................................................................. 47

9.1 TECHNICAL CHARACTERISTICS 47

10 MEMO............................................................................................................................................................ 49

11 GLOSSARY................................................................................................................................................... 53

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BASIC

1 REMARKS

The Automatic aspiration system kit is manufactured by SECOMAM.
The information contained in this document may be the object of patents or patent applications by

SECOMAM. The possession of this document does not confer any license to such patents.
The following names are SECOMAM’s registered trademarks throughout the world.

SECOMAM
BASIC

This manual is updated periodically. The updates are included in the new editions.
All information supplied in this edition of the manual may be amended before the products described

herein are available.

All reproduction rights reserved.

Reference Date of last

modification

Version Date of document. Writer Controller Approving

0NVBAU 07/12/04

1.19

JM

- 5 -

BASIC

2 WARRANTY

The new equipment and material sold by SECOMAM is guaranteed against any manufacturing defects
for one year (unless otherwise stated by SECOMAM) with effect:

− From the technical acceptance of the equipment in the factory by the buyer or his designee,

− or failing this :

* For Metropolitan France: from the date on the delivery note.
* For other destinations: from the date of factory shipment certified by air waybill,

consignment note or bill of lading.

The SECOMAM company guarantee applies exclusively to defectiveness arising from a design fault or
from a concealed defect. It is strictly limited to the free dispatching of replacement parts (except for
consumable items) or to the repairing of the equipment in our workshops within a deadline of 10
working days (shipping delay not included).

By express agreement, the following are strictly excluded from our guarantee:

− All damages, notably for staff costs, loss of earnings, business trouble, etc

− Any breakdown due to an incorrect use of the equipment (non adapted mains, fall, attempt at

transformation, etc) or to a lack of maintenance by the user or to poor storage conditions.

− Any breakdown due to the use of parts not supplied by SECOMAM, on SECOMAM equipment

− Any breakdown due to the transporting of the equipment in packaging which is not its original

packaging

− The lamps, the cells and generally any item which appears in the "accessories" section on the
price list.

Our customers are kindly asked to apply for our consent before returning any instrument for repair. No
return of materials may be accepted without the prior written consent of our Sales Management which
will precise the terms of such return.
If the above consent is given, articles shall be returned in their original packaging on a prepaid basis to
the following address:

SECOMAM - 91 avenue des Pins d’Alep – 30100 ALES FRANCE

We reserve the right to reship all instruments received collect failing such consent.
Whatever method and conditions of transport are chosen for the shipment of the equipment to be

repaired under guarantee, in the original packaging, the corresponding costs and the insurance costs
will be payable by the customer.

Any damage connected to the return transport of the equipment falls within the framework of the
guarantee on the express condition that the customer has sent his complaint within forty-eight hours
by registered letter with acknowledgement of receipt to the carrier. A copy of the letter should be sent
to SECOMAM.

For equipment with a guaranty card, this is only applicable if the card delivered with the equipment is
returned to SECOMAM duly completed.

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BASIC

SOFTWARE GUARANTEE

The software is guaranteed by the designer or the distributor of the software under the conditions
specified in the literature accompanying the aforementioned software packages.
Under no circumstances whatsoever will SECOMAM supply any type of guarantee for software
packages.
By express agreement, all damages, notably for staff costs, lost of earnings; business trouble, etc are
strictly excluded from our guarantee.
The customer is informed that the software cannot be guaranteed exempt from defects or bugs.

TRADE SECRET AND PROPERTY RIGHTS

This document is protected by a SECOMAM copyright (c) 2003 and the copying rights are explicitly
reserved. The software supplied with the equipment or referenced contains trade secrets and
confidential information which are SECOMAM's property. It is legally protected by the international
copyright (c) laws.

SECOMAM grants a license to use its software to the user. This may not be disclosed, used or
duplicated with the intention to save it, without SECOMAM's written permission. The beneficiary must
attach a copy of this document to all authorized partial or total reproductions.

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BASIC

3 INFORMATION

The SECOMAM equipment has been designed, manufactured, tested and inspected according to the
ISO 9001 standards.

If the unit is not immediately installed, it should be stored in a dry and clean area. The storage
temperature should be between 10 and 35°C.

SECOMAM equipment is carefully inspected before it is packed. As soon as you receive your
equipment, check the condition of the packaging and if you notice any problems, notify your carrier
within 48 hours. Then consult the packing list and check that everything is in order. Finally, if you
discover that something is missing, or if the goods are damaged immediately notify SECOMAM.

IMPORTANT:
In order to benefit from SECOMAM’s service (application notes, SECOMAM information, technical
assistance, etc.) immediately complete the attached guarantee card and return it to the following
address:

SECOMAM

Service PRODUIT

91 Avenue des Pins d’Alep

30319 ALES Cedex

FRANCE

Tel: [phone]

Fax: [phone]

E-mail: [e-mail]

Service Department: [phone]

Technical support: [phone]

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BASIC

4 PRECAUTIONS OF USE

Always make sure that the instrument is connected on the good voltage.



(Between 100 – 240V 50-60Hz)

 Always disconnect the mains plug before starting any work inside the

instrument.

 When dangerous substances for health and environment are used, the

laboratory or site rules, where the instrument is installed must be
followed.



Take all the necessary precautions, during the use the instrument, to
protect the operator from eventual liquids leaks or spills or possible
radiations (protective gloves, anti radiation glasses , protected clothes,
etc)

All operations made inside the instrument, must be done by SECOMAM or by

SECOMAM’s authorized technicians.

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BASIC

5 PRELUDE

The BASIC was designed within the framework of In Vitro Diagnostic to cover the need of
semi-automatization in clinical biochemistry analysis.

It includes the principal functions required by this kind of analysis such as:

- Wavelength range 340-700 nm,

- Peltier thermostated cell holder

- Calculation method adapted to enzymatic reading of results and parameters

- Quality control management

- Partial management of laboratory practice

BASIC complies with the requirements in CE marking of medical devices for In Vitro
Diagnostics (98/79/EEC)

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BASIC

6 DESCRIPTION

6.1 GENERAL OVERVIEW

Front view :{

{ XE "Screen" }{ XE "Keyboard" }

6.2 SCREEN AND KEYBOARD

XE "Front view" }

2

1

3

5

4

← Program keys +

number keys.

↑ Control keys.
→ Screen.
↓ Function keys.
° Cursors.

Function and screen keys

Thanks to these keys, working
with BASIC is simplified. Screen
instructions as well as the
function keys guide you
throughout the analysis.

On the display, Function keys
correspond to an icon or a value.
Simply validate by a light stroke.

:

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BASIC

• Cursor keys
other functions of the keyboard such as :

{ XE "Cursor" }{ XE "Program keys" }

Program keys

secondary position (secondary position are M1 to M47). These program keys are preassigned to give
you an idea of the program capacity and calculation method the instrument is capable of. You can
modify any given test method at any time, experiment your own parameters, place it at a different key
than the original and call it just by one stroke. Before modifying, it is recommended to print the existing
test method.

Number keys

(date, hour, sample values, sample numbers, ...).
{ XE "Number keys" }

: each program key can contain two test methods : on the primary position and the

: otherwise indicated on the display, numbers are to be entered by way of program keys

: Use these keys to have access to

- elevator mouvement : BASIC test
methods on the primary and
secondary positions, next screen
display,

- right arrow for double rinse

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BASIC

)

• BASIC’s software control keys :

6.3 DISPLAY DESIGN

Programmed

temperature

Validate an operation

Quit active screen

Reach software configuration screen

Erase & correct data

Test name

Calculating method

Concentration value,

measuring unit

Name of sample measured

(standard, sample X ...)

When using cuvette by cuvette method, you will see :

While for pumping aspiration :

Absorbance value

(blank value

deducted

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BASIC

7 SET-UP

7.1 GENERAL SET-UP

{ XE "General set-up" }
Step-by-step set-up of BASIC is done through the « CONF »key

CLOCK

Use (F1) to (F5) keys to modify

the values. Abort modification

with ESC key, register with VAL

key.

CONF

PRINTER

Access to set-up
menu for the printer

STAND-BY

Access to a sub-menu

allowing to choose the

duration before starting the

stand-by (screen

DATA FILES

Access to : -Patient
identification –Patient
report –Data archiving
to PC or Printer –
Memory reset.

saver)

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BASIC

Access to program

NOTE :

modification

PUMP

.

QUALITY CONTROL

Confirm (F1) or skipping (F4) the

quality control function. Display (F3)

values and dates of previous control.

Access to pump set-

up menu

CONFIGURATION screen is active when you see an elevator in
the middle of the screen. Use upward and downward cursor
keys to activate and have access to the next display screen.{
XE "Elevator" }

7.2 LANGUAGE SET-UP

This would be the first configuration to check upon starting the instrument. Successively press
« CONF », « downward cursor key », (F1) « Language set-up » then (F3) « English ». { XE "Language
set-up" }

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BASIC

Y

7.3 DATE AND CLOCK SET-UP

D+1

M+1

+1

Use the corresponding function key to increment date and time. Then « VAL » to { XE "Date set-up" }{
XE "clock set-up" }store, « ESC » to return to previous display.

7.4 PUMP SET-UP

{ XE "Pump set-up" }

Access to pump functions & set-up : debit , dead volume,
aspiration volume.
For your convenience, these volumes are factory
preadjusted.
(Note : upon changing pump and tubings , you have to
readjust setting).

Press (F3) « PUMP SET-UP » , you will obtain :

T+1

M+1

Press (F5) « NO » for manual cuvette by cuvette operation (recommended to use on highly­contaminant tests).
By answering (F2) « YES », you obtain three choices :

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BASIC

Pump rate (calibration) :

Prepare ahead :

• four test tubes with 2000 µl of distilled water each,

• one test tube with 1000 µl of distilled water to use as standard.

• One graduated pipette (vol. up to 1000 µl)

To adjust pump volume, you need to: { XE "Pump calibration" }

• Return to « CONFIGURATION ».

• Go to menu « PUMP ».

• Use pump « YES ».

• Exit from « CONFIGURATION » (ESC twice).

• Rince twice consecutively (using right cursor button) to fill the tube with water.

Then :

• Again return to « CONFIGURATION »,« PUMP »,« YES ».,« PUMP RATE »

• Introduce the first tube containing 2000 µl of distilled water to the aspiration noozle.

• Press (F3) « SUCK » and « VALidate » the message of 1000 µl. The pump should have aspirated

1000 µl. Compare the remaining volume with the volume of standard tube. Or better yet, aspirate
the remaining distilled water with the pipette and verify the volume. If the remaining volume is more
(or less) than 1000 µl, « DELete» the message of 1000 µl on display and enter the real volume
sucked by the pump using the number keys (calculation example : initial volume of 2000 µl –
remaining volume = new volume to program). Introduce the second tube containing 2000 µl of
distilled water.

• Press (F3) « SUCK » and « VALidate ». The remaining volume should be 1000 µl, same as the
volume of standard tube (or verify again with the pipette). If another adjustment is to be done,
repeat this process untill the instrument aspirated the right 1000 µl volume.

Always validate at the end of process by (F6) «OK». (To keep previous volume, press « ESC »).

Delay :

Is the frequency (in seconds) to program for sample introduction following one re ading.
You can work faster by shortening pump aspiration delay when you are more familiar with the system.

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BASIC

beginning of the dead volume

Dead volume

Sample

Joint

Flow through cell

.{ XE "Volume" }
200 µl is the factory preprogrammed volume:

Dead volume :

Dead volume is needed to avoid contamination of samples.
It is represented by a certain volume of air. Dead volume is
influenced by the length of aspiration tube. It is
recommended to program dead volume in such a way that
the end of the aspirated sample remains approximately 2
cm on top of the cell tip (see drawing).
{ XE "Dead volume" }

Upon selecting F3 Dead Volume, you will get a choice of 6
volumes.

7.5 PRINTER SET-UP

{ XE "Printer set-up" }

Select « PRINTER OPTION » by pressing F3.
Printer should be an 80 column or has an 80 column set-up.

When using a printer, select (F2)« YES », (F1) « TYPE » , enter the type of printer.
A selection of 4 printers are proposed : a KYOLINE, CITIZEN IDP562S, HEWLETT PACKARD and

EPSON. Choose one. Next, check the type of port « connection » either serial RS232C or Parallel.
Connect IDP or KYOLINE printer using its serial

printer cable to BASIC’s serial RS232C port.

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BASIC

Warning : never connect a serial printer with a 25-pin cable to the parallel port. This will do serious
damage to BASIC and the instrument will emit a continuous beeping noise. This type of error is not
covered by the warranty.

Exit from set-up by « ESC ».

7.6 TRACEABILITY SET-UP

{ XE "Trace-ability set-up" }

Data identification, filing, report etc. are given in this (F6) TRACE-ABILITY function :
F1 BASIC - PC : this function allows you to download data (patient reports) to the PC
(see chapter 5.6.1)
F2 BASIC – PRT : use this function to download data to a printer. Two types of printed
documents can be obtained :

• global daily report of all patients (enter Nr 0) or

• patient by patient report (selectable : example Nr 114, 121).

You can print previous day’s result (if it is not crushed/erased yet) by reprogramming the date (see
CONFIG, DATE).
Note :For two samples bearing the same number, only the latest will be edited.
F3 INIT is used to clear result memory. Be sure to save/print results before deleting.
F4 YES : by choosing this function, BASIC will report kinetical reading points per
segment.
F5 NO : by this function, BASIC will give brief kinetical report.
F6 ORDER is to select the printing order of a given patient, for example Nr 114 first then
Nr 121.

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BASIC

7.6.1 Downloading to a PC : F1 « BASIC –PC »

Connect BASIC to the PC using ref : 0X6807 cable and if needed, an adaptator.

{ XE "Download to a PC (Win 95)" }

FOR WINDOWS 95

♦ Click on « START », PROGRAM », « ACCESSORIES », « HYPERTERM », windows 95 opens

a window where you will find «

♦ Open « HYPERTRM.EXE » which will give access to other window « NEW CONNECTION »

and «

DESCRIPTION OF A CONNECTION ».

♦ Choose the name and icone of this connection (bearing in mind the type of instrument

which is your source of data) then «

♦ You’ll see « DESCRIPTION » disappear to leave space to a new windows called « TEL

NUMBER », also « NEW CONNECTION HYPERTERMINAL » will appear as « NAME OF
INSTRUMENT

plugged. Validate «

-HYPERTERMINAL ». Select the COM number where the connecting cable is

OK ».

♦ When « PROPERTY COMx » shows up, and you should feed the following informations

:

Bits / second : 9600
Data bit : 8
Parity : none
Stop bit : 1
Flux control : none

♦ Then validate « OK ».
♦ Check that option « ADD LINE MODIFICATION AT THE END OF ENTERING LINES », in menu

«

FILES », « PROPERTY » index « PARAMETERS » button « CONFIG ASCII » at framed

level « ASCII

RECEPTION » is activated.

♦ To save the parameters, select « FILES » then « SAVE ». Windows register selected

parameters under predefined name.

♦ To receive result file from the instrument, click on « TRANSFER » then « CAPTURE

» menu.

TEXT

HYPERTRM.EXE ».

OK ».

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BASIC

♦ Windows opens a dialog box « CAPTURE TEXT ». Choose in which repertory you will

save the data result file also under which name, either by entering the pathway or the
name with « .

♦ Type « start », and your terminal will be on reception mode.
♦ On BASIC itself, press « BASIC-PC » button, you will see its data on the PC screen.

Open « TERMINAL » program which is usually under program group « ACCESSORIES ».
To create acquisition program, do the following :

Select «
option.
Then select «

Continue by selecting «
follows :

{

XE "Download to a PC (Windows 3.x)" }

Then choose COM number where the connecting cable is plugged.
To register the parameters, select «

box «
name with « .

To receive result file from the instrument click menu «

TEXT FILE

A dialog box «
save the data result file, also under which name with « .
your terminal will be on reception mode.

• On BASIC itself, press « BASIC-PC », data will appear on PC’s screen.

SAVE ». Choose in which repertory you want to save the program, also give a

TXT » extension, or by selecting « FOLLOW » icone.

FOR WINDOWS 3.x

PARAMETERS », « TERMINAL EMULATION » and select « DEC VT 100 (AINSI) »

PARAMETERS », « TERMINAL PARAMETERS », then the following options :

Character terminal : terminal
column : 80

CR > CR/LF ⌧ Entry  Exit

PARAMETERS » « COMMUNICATION » and define the page as

transmission rate : 9600 bauds
Data bits : 8 bits
Stop bits : 1 bit
Parity : none
Flux control : none
Parity control :  (non selected)
Beared detection :  (non selected)

FILE » then « SAVE ». Windows will open a dialog

TRM » then enter « OK ». That file name is shown on PC’s title bar.

TRANSFER » then « RECEIPT A

».

RECEIPT A TEXT FILE » is opened. Choose in which repertory you will

TXT » extension. Press « OK »,

7.6.2 Downloading to a printer : F2 « BASIC-PRINTER »

{ XE "Download to a printer" }

Connect BASIC to a printer.
Press « B
You will obtain :

ASIC-PRT ».

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BASIC

1 2 3 4 5

M 47;E; 0 0.0;%; 29/08/1997;08H17; -;?
M 47;E; 1 210.4;%; 29/08/1997;08H19; L;?
M 47;E; 2 -0.0;%; 29/08/1997;08H19; ;?
M 47;E; 3 -0.0;%; 29/08/1997;08H20; ;?
M 47;E; 4 9.9;%; 29/08/1997;08H20; ;?
M 47;E; 5 9.9;%; 29/08/1997;08H20; ;?
M 47;E; 6 9.8;%; 29/08/1997;08H21; ;?
M 47;E; 7 9.8;%; 29/08/1997;08H22; ;?

Column 1 : Sample number + characteristics :

B = Reagent blank,
S = Standard,
E = Sample,
C = Control,
b = Blank of reagent blank,
s = Standard blank,
e = Sample blank,
c = control blank.

Column 2 : Sample number.
Colonne 3 : Concentration value and unit.
Column 4 : Date and time.
Column 5 : Status :

- : No problem,

> : Result superior to normal value,
< : Result inferior to normal value,

L : result superior to linearity limit,

− : negative concentration value
B : Blank value superior to linearity limit,
Q : Control non performed,

! : Control badly performed,

- : Control normal.
Memory initialisation{ XE "Memory initialisation" }
To erase the results stored in memory, press « init ».

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BASIC

7.6.3 KINETICAL REPORT

On methods using KINETICS, you have a choice of having a short one line final reading report by
choosing F5 « NO » or segment per segment reading through F4 « YES ».

7.7 QUALITY CONTROL SET-UP

Each Quality Control runned is memorized automatically.
F4« NO » gives you the choice not to run QC.
F3« DISPLAY » allows to display on screen as graph the values and dates of previous control (only
after at least two controls of the same analysis method have been made). Printout of QC can be
obtained after programming TRACE-ABILITY function, YES.
{ XE "Quality control set-up" }

« NO ».

Upon validating (F1) « YES » , you will be reminded to run
Quality Control later on upon running a test method (CHECK
ANALYSIS) and flags will be given according to the
« MAXIMUM & MINIMUM TOLERANCE VALUES » entered.
Q preceding result indicates that QC was not performed.

Note : Upon using a test method for the first time in the day,
you will be suggested to run a control. However, once QC is
done for the day, no need to run another one, hence F6

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BASIC

This control can be done at a regular interval, defined in days :

To do so, return to « PROGRAMMING », F1« MODIFY », select the corresponding analysis method,
and validate till you obtain the following display :

{ XE "Control frequency" }
Enter the frequency (in days) and validate . This procedure determines QC periodicity. Control can be
done daily, for 31 days.

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BASIC

This way, daily/periodically, the instrument memorizes analysis results & quality controls.

Status of control

Use upward/downward

Chronological filing of

control

Printing control results

Exemple of print-out (analysis comprising one quality control per day :

SECOMAM - Polyphotometer UV-Vis BASIC V1.11
Date : 04/06/1997 Hour : 09H25

User :

Analysis : ALBUMIN 37°C

Sample Abs. Concentration Status

arrows to visualize

controls .

Reagent Bl. 0.000
Standard 0.100
Control 0.150
OK Control - Le 05/06/97

Normal value: 38.00 à 54.00 g/l

S. # 1
S. # 2

Std. # 3
Std. # 4
Std. # 5

0.150

0.300

0.700

1.300

1.250

0.00 g/l

1.00 g/l

1.50 g/l OK

1.50 g/l

3.00 g/l

7.00 g/l

13.00 g/l

12.50 g/l

<
<

<
<
<

- 30 -

BASIC

SECOMAM - Polyphotometer UV-Vis BASIC V1.11
Date : 05/06/1997 Hour : 09H26

User :

Analysis : ALBUMIN 37°C

Sample Abs. Concentration Status

nd

2

day

Reagent Bl. 0.000
Contrôle 2.100
Control Pb Le 06/06/97

Normal value : 38.00 à 54.00 g/l

S. # 1
S. # 2

0.100

1.600

0.00 g/l

21.00 g/l PB

1.00 g/l

16.00 g/l

<
<

SECOMAM - Polyphotometer UV-Vis BASIC V1.11
Date : 06/06/1997 Hour : 09H27

User :

Analysis : ALBUMIN 37°C

Sample Abs. Concentration Status

Reagent Bl. 0.000
Control 0.990

Control 0.990
Control Pb Le 07/06/97

0.00 g/l

9.90 g/l PB

9.90 g/l PB

rd

3

day

Normal value: 38.00 à 54.00 g/l

Status :

> : Sample concentration superior to upper limit.
< : Sample concentration inferior to lower limit..

PB : Problem on control.
OK : satisfactory control.
Q : Appears next to analysis result when control is programmed but not runned.
! : Appears next to analysis result when the control is out of tolerance range.
L : The result in concentration is no longer within the linearity limit of the analysis.

− : The result in concentration is negative.

Upon modifying analysis method, memorized results are erased.

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BASIC

8 PROGRAMMATION

Through configuration, select « PROGRAMMING » to modify a preset analysis method or to create a
new one.

Programmable parameters are :

− suction volume,

− temperature,

− unit

− accuracy,

− calculation mode,

− analysis mode

− time (lag time, interval time and number of intervals),

− wavelength,

− linearity limit,

− normal range (high/low)

− check frequency,

−

Note : At any case, arbitrary values are labeled by small arrows on the right or the left side of the
value.

{ XE "Program keys" }

8.1 PROGRAM KEYS

To program an analysis test, simply return to set-up procedure : « CONFIGURATION », then
« PROGRAM », PROGRAMMING : F1« MODIFY » :

Next you will obtain the « ANALYSIS » selector panel : simply press ↑ or ↓ or cursor key for access to
program keyboard.

previous results & standard control will be crushed upon saving a new method.

Primary position Secondary position

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BASIC

Select one of the program key to place the new test method.

ADJUSTMENTS RELATED TO ANALYSIS

Name the analysis method should be done first. It is recommended not to use the same

name as the one found in the primary position. DELete test method name and replace. Choose letters
and numbers with F1 to F6 keys and ↑ or ↓ cursor. Validate at the end.

Aspiration volume :

 800 µl is the recommended pump aspiration volume. However a selection of six volumes from 500

to 1500 µl are available.

To modify the volume, press the F key corresponding to the volume to program.

Temperature :

{XE "Temp"}
BASIC is equiped with a Peltier-effect temperature control cell-holder.
Recommended temperature to use is given by the test method technical sheet.
Use F key to program the temperature. Note :during warm-up, the programmed temperature digits will
blink, rather fast at first, slowing down afterwards. It takes 15 minutes to stabilize the cell-holder and
cuvette to 37°C Once temperature is reached, the digits will be displayed permanently.
Peltier-effect is deactivated when programmed at XX (Room temperature/F1)
Note on work organization : it is adviced to run a ll test methods using the same temperature in one
session to avoid warm-up delay. For your convenience, the cell-holder will remain on the last

Room temp

temperature used.

Unit :

Choose measuring unit according to the recommendation of the reagent technical sheet.

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BASIC

{ XE "Unit" }

Result resolution (decimals) : Concentration result is given by the number of decimals

programmed.

{ XE "Result resolution" }

Calculation mode :

{ XE "Calcul mode" }
A choice of three calculation modes are proposed :

F1 Factor

Enter factor value indicated on the reagent technical sheet (DELete, then enter numbers). Results will
be calculated using this factor.

: in Kinetic mode, enzymatic factor (coefficient) value is used instead of calibration factor.

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BASIC

F2 Linear : In End-point, End-point with blank and Two-points-kinetics analysis modes,

calibration is needed (which means, the calculation is done using standard(s)). Enter
standard(s) value(s) (DELete, then enter numbers).

F3 Non-linear

: E.I.A tube methods use this calculation mode (polynomial, log/logit, spline, multi-

points). Enter the number of standards used, then the values of each. Up to 6
standards can be used.

Wavelength :

{ XE "Wavelength" }

Wavelength to use is indicated by the reagent technical sheet. You may not find the exact same
wavelength among those proposed by BASIC. In that case, select one closest to the ideal
wavelength .

{ XE "Analysis mode" }

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BASIC

∑∑∑

Analysis mode :

End point without

blank serum

End point with

blank serum

Enzymes kinetics

.F1. End-point analysis mode :
When using this mode, reading is done at the final absorbance (optical density) point (at the
end of the reaction process). Zero is adjusted using reagent alone (reage nt blank) or
distilled water.

F2. End point with
A number of environments, in particular biological ones, may be the object of
spectral interferences at the wavelength selected for the dosage. This is the case for
colored molecules (haemoglobin, bilirubin, ...) These interferences which are independent of the
parameter to be measured, will be eliminated by measuring the sample alone or with a reagent which
does not trigger a coloring effect. : this is called a sample/serum blank.
When there is only one reagent and such a reagent may add a parasitical color to the wavelength of
the reading, it is deducted from such reading; this is referred to as reagent blank.

End point with serum blank:

Conc. Std = (abs. Std – Abs. reagent Blank) x coef. Calibr.

End point without serum blank:

Conc. Std. (Abs. Std.- Abs. Serum Blank )- (Abs. Blank - Abs Blank blank)= Coef .

F3 Enzyme kinetics{
method calculates the evolution of a sample, in Absorbance, against a time period
defined in a certain number of intervals. BASIC computes the straight regression line which will
average the means of the points.
BASIC allows you to select the duration of analysis reading as well as the number of intervals to be
measured. Also lag time between each intervals should be indicated.

[]

XE "Enzyme kinetics"} : Called also zero order kinetics. This

: {XE "End point with serum blank "}

.{ XE "End point without blank serum" }

slope

N : Total number of point of the kinetic curve (nb of segments x segment time).

ψX : 1+2+3+...+N x (N+1) /2
ψY : total of absorbances at points 1, 2, 3, ... N=A+A2+A3+...+AN
ψXY : (A1 x 1)+(A2 x 2)+(A3 x 3)+...+(AN x N)

2

ψX

: 1+4+9+...+N2 = N(N+1)(2N+1) /6

The slope thus calculated is expressed in delta of Abs./min.

F4 Kinetics with standards{
first order kinetics. This analysis method allows to perform measurements of Concentration
in Kinetics mode. That is, to calculate the absorbance variation of a solution within the time

and to multiply the differences of Absorbance by a coefficient or to compare to a standard
measured with the same measuring conditions.
Thus, global waiting time of measurement should be indicated. Then BASIC requires confirmation on
the time consumed between the two point reading.

blank:

()( )

Nx XY Xx Y

()()

Nx X X

XE "Kinetics with standards"} : Called also two-point kinetics or

−

22

−

∑∑

Kinetics with

standards

Bichromatism

x=

60

- 37 -

BASIC

ABS ABS x f x

R

=

ABS t1 : Abs of the solution at the end of the lag time.
ABS t2 : Abs of the solution at the end of the measuring time.
factor : Coefficient of molecular absorption.
tm : Measuring time.
60/tm : Ratio reducing the result to one minute.

The slope thus calculated is expressed in delta of Abs./min.

F5 Bichromatism
Reading is done using 2 wavelengths multiplied by a factor.

Abs Smpl Abs. Smpl.

.

()

: {XE "Bichromatism"}is recommended when sample is cloudy (turbid).

− F

12

λλ

−() t t2 actor 160

tm

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BASIC

For both KINETIC modes, enter Lag time (the waiting time before reaction starts), number of intervals
and time segments of each interval. Indicate also whether it is an increasing/decreasing type of
reaction.

5

10

- 39 -

BASIC

Linearity limit :

{ XE "Linearity limit" }

The maximum measuring limit is given in the reagent technical
sheet. If result is beyond this limit, it is recommended to dilute the
sample or rerun the test.
CAUTION : in hyper-active samples having decreasing kinetic-type
of reaction, total substrate can be consumed before first measure
starts. (Shown by reading result close to zero, below the depletion
limit). It is user’s responsibility to interprete reading result taking into
account this element.

Normal high : Normal High & Low values border the acceptable pathological range. It is also

called the usual expected values. Flags will be shown on abnormal result.

{ XE "Normal high" }

Normal low:

{ XE "Normal low" }

CHECK FREQUENCY:

At the end of programming, you are asked the frequency of analysis quality control checking : whether
daily or periodically. A signal will remind you to do the quality control every day or once every x days.
Result of the QC can be found at « CONFIGURATION », « QUALITY CONTROL », and you can ask
for « DISPLAY ».

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BASIC

8.2 RECOMMANDATION FOR DAILY ANALYSIS

Zero measurement will be asked at the beginning of each day.

SAMPLE ASPIRATION :

Introduce tube to the noozle upon
instructed by display. Audio beeper
indicates aspiration. Remove tube as
soon as aspiration ceased. Resume
with next sample simply by introducing
the tube.
In case of aspiration detector failure,

MODIFICATION OF STANDARD VALUE :
Standard concentration value can be modified during analysis.
SINGLE STANDARD : use F3 « MODIFY ».{XE "Single standard"} Enter new standard value.

use F1 button to start aspiration.

MULTI-STANDARDS : { XE "Multi-standard" }

Confirm whether standard values to use are as programmed
(« OK ») or remeasurement are to be done (« Repeat »).

- 41 -

BASIC

If you confirm the latter, you will see :

Modification of each standard value can be done through the
corresponding F button.

If the values on display are correct, validate.
Otherwise modify using numerical keys, then
validate.

Start with reagent blank reading. This will be deducted automatically
from other readings :

Measuring results will be displayed as :

The standards will be called one after another to establish standard curve to calculate sample values.

- 42 -

BASIC

As soon as the standards are read , the curve will be displayed. Absorbance values of standards are
also displayed.

By validating F6 « OK », the values are stored in memory.

8.3 ERROR MESSAGES

{ XE "Error messages" }

Sample absorbance value is superior to 2,5 (above the reading limit
of BASIC) : in this case the software will show « -,--- ». Check any
possible manipulation errors : the sample not prepared correctly or
the cleanlinest of the cuvette.

The result in Concentration is superior or inferior to
the normal (high or low) value. In this case, a sign
« < » or « > » will be displayed following the result.
Negative Concentration value will be indicated by the
sign −.

The result in Concentration is no longer in the linearity limit of the
analysis : another sign « < » shows up in front of the result (in this
case the result is also beyond the normal high or low range).

Non executed quality control procedure will be signaled by « Q».

During decreasing kinetics method, if the difference of Optical
Density between the initial O.D. at T0 and at T1 (end of the latent
time) is superior to the depletion limit, the software does not display
any result.

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BASIC

If the initial O.D. value of the blank is superior or inferior to the limit ,
a warning sign appears following the concentration result.
If the Quality Control value is beyond the specified limit, the sign
« ! » appears in front of the Concentration result.
In case of Peltier failure Tsign appears instead of the temperature
programmed.

PATIENT REPORT & PRINT-OUT

PATIENT REPORT :
Daily analysis are memorized automatically by BASIC and indicated at the bottom of the result display
as a blinking line. User is recommended to empty the memory at the end his working session.
This can be done by :
transferring all data to print ( see chapter 5.6.2.) or
downloading all data to file on a PC ( see chapter 5.6.1.) or
selecting particular data to print (see chapters 5.6.2 & 5.6.3.). In this case, remember to
use patient number as selection criteria. Note : the latest of sample bearing identical number will be
reported.

{XE "Print out"}
When quality control is programmed, error messages will be given on « status » column.

SECOMAM - Polyphotometer UV-Vis BASIC V1.11
Date : 04/06/1997 Hour : 09H24

User :

Analysis : ALBUMIN 37°C

Regeant Bl. 0.000 0.00 g/l
Standard 1.000 1.00 g/l
Control 0.900 0.90 g/l

Sample

Abs.

Concentration

Status

PB

- 44 -

BASIC

SECOMAM - Polyphotometer UV-Vis BASIC V1.11
Date : 04/06/1997 Hour : 09H24

User :

Analysis : ALBUMIN 37°C

Reagent Bl. 0.000 0.00 g/l
Std 1.000 1.00 g/l
Control 0.600 0.60 g/l
Control 0.990 0.99 g/l
Control 0.990 0.99 g/l
S. # 0.500 0.50 g/l
S. # 0.500 0.50 g/l
S. # 1.200 1.20 g/l
Std. # 2.000 2.00 g/l

When everything goes well OK will be indicated:

SECOMAM - Polyphotometer UV-Vis BASIC V1.11
Date : 04/06/1997 Hour : 09H24

User :

Analysis : ALBUMIN 37°C

Bl. Reagent 0.000 0.00 g/l
Sample 1.000 1.00 g/l
Control 1.500 1.50 g/l

Sample

Standard

Abs.

Abs.

Concentration

Concentration

Status

Status

PB
PB
PB

<
<
<
<

OK

8.4 SAMPLE NUMBERING

Sample numbers will be incremented automatically. However you can assign another number through
F2 key. Example : Sample # 3 is to be modified. Press F2, and you will see Sample Nr.1 on the
screen. Press Delete and replace by the number you want using number keys.

- 45 -

BASIC

9 MAINTENANCE

9.1 TECHNICAL CHARACTERISTICS

{ XE "Technical characteristics" }

PARAMETERS IN MEMORY 94
READING MODE SIMPLE ABSORBANCE
END POINT WITH SERUM BLANK
END POINT WITHOUT SERUM BLANK
REAL KINETICS
KINETIC TWO POINTS
BICHROMATISM
CALIBRATION LINEAR / NON LINEAR
6 STANDARDS
LIGHT SOURCE HALOGEN LAMP
MONOCHROMATOR HOLOGRAPHIC GRATING
DETECTOR SILICIUM DIODE ARRAY
SAMPLE COMPARTMENT DRY TEMPERATURE PELTIER SYSTEM
OPERATING TEMPERATURE AMBIENT - 25 - 30 - 37°C
TYPE OF CUVETTE FLOW-THROUGH µCUVETTE 30 µl

10 mm OPTICAL PATH
ASPIRATION VOLUME PROGRAMMABLE, 500 to 1500µl
QUALITY CONTROL MANAGEMENT OF QC RESULTS/MONTHS
POWER REQUIREMENTS 110 / 220V
INTERFACE SERIAL RS232C AND PARALLEL
CERTIFICATION AND

APPROVALS
LANGUAGES MULTILINGUAL

ISO 9001 AFAQ N°1995/4102 - CE - EMC

- 47 -

BASIC

10 MEMO

BASIC

- 49 -

Note down the analysis names you have modified.

SUBSTRATES ENZYMES

M1

..................................

M4

..................................

M7

..................................

M10

..................................

M13

..................................

M16

..................................

M19

..................................

M22

..................................

M2

.....................................

M5

.....................................

M8

.....................................

M11

.....................................

M14

.....................................

M17

.....................................

M20

.....................................

M3

.....................................

M6

.....................................

M9

.....................................

M12

.....................................

M15

.....................................

M18

.....................................

M21

.....................................

M23

..................................

M25

..................................

M27

..................................

M29

..................................

M31

..................................

M33

..................................

M24

.....................................

M26

.....................................

M28

.....................................

M30

.....................................

M32

.....................................

M34

.....................................

Note down the analysis names you have modified.

SUBSTRATES ENZYMES

Albumin

..................................

Anti Thrombin

..................................

Calcium

..................................

HDL Choles.

..................................

Glucose

..................................

RBC Magnesium

..................................

T. Protein

..................................

Uric Acid

..................................

Alcohol

.....................................

Bicarbonate

.....................................

Chloride

.....................................

LDL Choles.

.....................................

Iron

.....................................

Phos. Lipids

.....................................

Triglycerides

.....................................

Ammonia

.....................................

Bilirubin

.....................................

Cholesterol

.....................................

Creatinine

.....................................

Magnesium

.....................................

Phosphorus

.....................................

Urea

.....................................

Acid Phosphat.

..................................

Amylase

..................................

Apo-B

..................................

Alat / GPT

..................................

Gamma GT

..................................

Lipase

..................................

Alk. Phosphat.

.....................................

Apo-A1

.....................................

Asat / GOT

.....................................

Ck. NAC

.....................................

LDH

.....................................

Nucleotidase

.....................................

BASIC

11 GLOSSARY

A

Analysis mode 30

M

Memory initialisation 21
Multi-standard 35

B

Bichromatism 32

C

Calcul mode 29
clock set-up 15
Control frequency 23
Cursor 10

D

Date set-up 15
Dead volume 17
Download to a PC (Win 95) 19
Download to a PC (Windows 3.x) 20
Download to a printer 21

N

Normal high 34
Normal low 34
Number keys 10

P

Print out 38
Printer set-up 17
Printer set-up 17
Program keys 10, 27
Pump calibration 16
Pump set-up 15

Q

Quality control set-up 22

E

Elevator 14
End point with serum blank 31
End point without blank serum 31
Enzyme kinetics 31
Error messages 37

F

Front view 9

R

Result resolution 29

S

Screen 9
Single standard 35

T

G

General set-up 13

Technical characteristics 41
Temp 28
Trace-ability set-up 18

K

Keyboard 9
Kinetics with standards 32

L

Language set-up 14
Linearity limit 34

U

Unit 29

V

Volume 17

W

Wavelength 30

- 53 -