USER MANUAL
D3024 & D3024R
High Speed (Refrigerated) Micro-Centrifuge
SCILOGEX, LLC
www.scilogex.com
Before using centrifuge, please carefully read this user manual for efficient operation and safety.
USER MANUAL
Contents
Copyright
Safety Reminder
1. Specifications
2. Declaration of Conformity
3. Required Operation Conditions
3.1 Basic Operation Conditions ..........................................................................................................................................7
3.2 Transport and Storage Conditions .................................................................................................................................7
4. Installation
4.1 Location ........................................................................................................................................................................7
4.2 Connection of the Power Cord and Grounding .............................................................................................................7
5. Structure
6. Operation Panel
7. Rotor Preparation
7.1 Prepare the Samples ....................................................................................................................................................12
7.2 Inject the Samples into the Tubes ...............................................................................................................................12
7.3 Keep the Tubes Balanced ............................................................................................................................................12
7.4 Inspect the Rotor .........................................................................................................................................................12
7.5 Symmetrically Load Centrifuge Tubes to Rotor .........................................................................................................12
8. Operation
8.1 Normal Operation .......................................................................................................................................................13
8.2 RCF Operation ............................................................................................................................................................16
8.3 Pulse Operation ...........................................................................................................................................................16
...........................................................................................................................................................................3
................................................................................................................................................................4
.....................................................
...........................................................................................................................................6
..................................................................................................................................7
.............................................................................
........................................................................................................................................................................8
..........................................................................................................................................................10
.........................................
.....................................................................................................................................................................13
............................................................................................................6
........................................................................................7
..............................................................................................................12
9. Maintenance
9.1 Cleaning ......................................................................................................................................................................17
9.2 Consumables ...............................................................................................................................................................18
9.3 The Replacement of Seal Rings ..................................................................................................................................18
9.4 Routine Inspection ......................................................................................................................................................18
10. T r oubleshooting
10.1 Possible Problems and Solutions ..............................................................................................................................19
10.2 How to Open the Door ..............................................................................................................................................19
10.3 Replacement of Fuses ...............................................................................................................................................20
11. Instructions for the Rotor and Tubes
11.1 Rotor Instructions ......................................................................................................................................................20
11.2 Tubes .........................................................................................................................................................................22
12. Calculate RCF
13. Ordering Information
14. Warranty
14.1 Warranty of the Centrifuge........................................................................................................................................25
14.2 Warranty of the Rotor................................................................................................................................................25
15. After-sales Service
................................................................................................................................................................17
.........................................................................................................................................................19
....................
...........................................................................................................................................................23
..............................................................................................................................................24
..................................
.....................................................................................................................................................25
..................................................................................................................................25
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Page 2
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High Speed (Refrigerated) Micro-Centrifuge
Copyright:
No part of this manual may be reproduced or transmitted without prior written permission of SCILOGEX.
We can not be responsible to inform at real-time if the outline and specifications of centrifuge are subject to change for improvement.
VERSION201104
www.scilogex.com
Page 3
USER MANUAL
Safety Reminder
Common safety precautions
Carefully read t
Follow the instructions and procedures described in this manual to operate this centrifuge safely.
Carefully read all safety messages in this manual and the safety instructions on the centrifuge.
Safety messages are labeled as indicated below. They are in combination with signal words of ĀWARNINGāand
ĀCAUTIONā with the safety alert symbol to call your attention to items or operations that could be dangerous
to you or other persons using this centrifuge. The definitions of signal words are as follows:
WARNING:Personal Danger
ʽ
Warning notes indicate any condition or practice, which if not strictly observed, could result in personal injury
or possible death.
CAUTION:Possible damage to centrifuge
ʽ
Caution notes indicate any condition or practice, which if not strictly observed or remedied, could result in
damage or destruction of the centrifuge.
NOTE:Notes indicate an area or subject of special merit, emphasizing either the productÿs capability or
common errors in operation or maintenance.
Do not operate this centrifuge in any manner not described in this User manual. When in doubt or have any troubles
with this centrifuge, ASK FOR HELP.
The precautions described in this User manual are carefully developed in an attempt to cover all the possible risks.
However, it is also important that you are alert for unexpected incidents. Be careful operating this centrifuge.
he following safety precautions for a thorough understanding.
ʽ
WARNING
This centrifuge is not explosion-proof. Never use explosive or flammable samples.
Do not install the centrifuge in or near places where inflammable gases are generated or chemicals are stored.
Do not place dangerous materials within 30cm around the centrifuge.
Prepare all necessary safety measures before usi
pathogenic micro-organisms. Use of these is at your own responsibility.
If the centrifuge, rotor and accessories that have been contaminated by solutions with toxic, radioactive or
pathogenic materials, clean it according to the decontamination procedure as specified.
If you require service at site, please sterilize and decontaminate the centrifuge in advance, and then notify the
service center the details of the materials and procedure.
To avoid electrical shocks, insure hands are dry before handling the power cord or turn on/off the power
switch.
For safety purposes, do not enter within 30cm around this centrifuge when it is in operation.
While the rotor is rotating, never release the door lock.
Unauthorized repairs, disassembly or modifying the centrifuge except by our service center are strictly
prohibited.
ng samples that are toxic, radioactive or contaminated with
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High Speed (Refrigerated) Micro-Centrifuge
CAUTION
ʽ
This centrifuge must be located on a firm and level table.
Make sure the centrifuge is horizontal before running.
Make sure the angle between the door and cover is greater than 70 degrees when opening the door.
Be careful not put your fingers or hands between the door and cover while the door is open.
Do not move or relocate the centrifuge when it is running.
If fluid spills in the rotor chamber, please promptly clean and dry with a dry cloth to avoid sample
contamination.
Ensure to remove any objects and fragments of the tubes dropped inside the rotor chamber before running the
centrifuge.
Cautions with rotor
(1) Always check for corrosion and damages on the rotor surface before using it. Do not use the rotor if an
abnormality is found.
(2) Do not set the speed beyond the allowable minimum speed of the rotor kits (rotor or adapters). Make sure to
run it below the allowable minimum speed.
(3) Do not exceed the allowable imbalance.
(4) Use the rotor and tubes within their actual capacities.
(5) If the rotor is attached with a lid, ensure it is tightened before operation.
If any abnormal condition occurs during operation, please stop it immediately and contact our service center.
Notify the service center is a warning code if displayed.
Vibrations are likely to damage the centrifuge, contact our service center if abnormality observed.
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Page 5
USER MANUAL
1. Specifications
Maximum speed 15000rpm(200-15000rpm), increment: 10rpm
Maximum RCF 21380hg, increment: 10hg
Maximum capacity 2mlh24, 0.5mlh36, 4-PCR8 serial tubes
Temperature range -20ć~40ć(D3024R)
Timer 30seconds -99minutes-HOLD, continuous operation
Driving Motor Brushless DC motor
Safety devices
Power requirements
Dimensions(mm)
Weight D3024: 12kg D3024R: 30kg
Additional features Speed/RCF switch; Pulse operation; Processing display; Voice reminder
Dual door interlock; Over-speed detector; Over-temperature detector; Automatic internal
diagnosis
D3024:
Single-
phase, 220V-240V, 50Hz/60Hz, 5A.
110V-120V, 50Hz/60Hz, 5A
D3024R:
Single-phase, 220V-240V, 50Hz/60Hz, 10A.
110V-120V, 50Hz/60Hz, 10A
D3024:(L)280h(W)364h(H)266
D3024R:(L)338h(W)580h(H)324
2. Declaration of Conformity
Construction in accordance with the following safety standards:
EN 61010-1
EN 61010-2-20
Construction in accordance with the following EMC standards:
EN 61326-1/ FCC Part 15 Subpart B/ IECS 001
Associated EU guidelines:
EMC-guidelines: 2004/108/EC
Centrifuge guidelines: 2006/95/EC
This ISM device complies with Canadian ICES-001.
Cet appareil ISM est conforme à la norme NMB-001 du Canada.
Changes o
authority to operate the equipment.
NOTE: This centrifuge has been tested and found to comply with the limits for a Class A digital device, pursuant to
Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference
when the centrifuge is operated in a commercial environment. The centrifuge generates, uses, and can radiate radio
frequency energy and, if not installed and used in accordance with the user manual, may cause harmful interference
to radio communications. Operation of centrifuge in a residential area is likely to cause harmful interference in which
case the user will be required to correct the interference.
r modifications not expressly approved by the party responsible for compliance could void the user's
Page 6
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High Speed (Refrigerated) Micro-Centrifuge
3. Required Operation Conditions
3.1 Basic operation conditions
1Power:
D3024: Single-phase, 220V-240V, 50Hz/60Hz, 5A; 110V-120V, 50Hz/60Hz, 5A
D3024R: Single-phase, 220V-240V, 50Hz/60Hz, 10A; 110V-120V, 50Hz/60Hz, 10A
2Ambient temperature: 2ć~40ć.
3Relative humidity: ≤80%.
4No vibration and airflow around.
5No electric dust, explosive and corrosive gases around.
3.2 Transport and storage conditions
1Storage temperature: -40ć~55ć.
2Relative humidity: ≤93%.
4. Installation
This section describes the instr
optimum performance. Before moving the centrifuge, the rotor must be removed.
ʽ
WARNING
Improper power supply may damage centrifuge.
Make sure the power source conforms to the required power supply before connecting.
4.1 Location
Place this centrifuge on a firmǃflat and level surface, ensure the four feet of this centrifuge stand on the counter firmly.
Avoid installing on a slippery surface or surface prone to vibration.
Ideal ambient temperature is 20ćf5ć, avoid placing the centrifuge in direct sunlight if temperature exceeds 30ć.
Keep clear of the centrifuge at least 10cm on both sides and at least 30cm behind it
Keep away from heat or water to avoid sample temperature issues or centrifuge failures.
4.2 Connection of the power cord and grounding
uctions that you should abide when install the centrifuge to ensure your safety and the
to guarantee the cooling efficiency.
ʽ
WARNING
To avoid electrical shocks, ensure your hands are dry when touching the power cord.
This centrifuge must be grounded properly.
A minimum 10A outlet providing a sufficient ground is required, and this must meet local safety requirements.
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Page 7
USER MANUAL
5. Structure
D3024
Seal ring of door
Operation panel
Door lock hook
Cover
POWER switch
Door
Figure 5.1 Front view of centrifuge D3024
View port
Air vents
Outlet
Page 8
Figure 5.2 Rear view of centrifuge D3024
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D3024R
High Speed (Refrigerated) Micro-Centrifuge
Door lock hook
Seal ring of door
Cover
Operation panel
Outlet
POWER switch
Figure 5.3 Front view of centrifuge D3024R
View port
Door
Air vents
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Figure 5.4 Rear view of centrifuge D3024R
Page 9
USER MANUAL
6. Operation Panel
Speed display area Temperature display area Time display area
5> 6
>B== = $
5? 6
}z $
$$$$$$$? B $¥$$$$ $$$$$$$$>?
zv{
5B 6
5@ 6
5A 6
Item
Symbol
1
2
3
4
5
Page 10
Name
Pulse button
Open/ lock button
Select button
Start/ Stop button
Program button
Function
The speed can be accelerated and held at the set speed when pressing PULSE button.
Press the button to open the door. The button is not available when the centrifuge is running.
Press the button to choose the program which you want to modify.
Press the button to start running. The centrifuge will brake and stop running if pressed during
centrifugation.
Clockwise rotate to increase program values. Rotate anti-clockwise to decrease program values.
Press the button, shift between speed and RCF display.
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High Speed (Refrigerated) Micro-Centrifuge
15000
Main interface of D3024 model is as figure 6-2. The speed is set to be 15000rpm, temperature of centrifugal chamber
is 25ć and the running time is 12 minutes. When speed symbol is rotating, this indicates the centrifuge is
running. If the rotation is faster, the speed is higher. Temperature of chamber is displayed and cannot be controlled.
Time symbol displays the ratio of working to time setting. The total time setting is divided into 10 sections.
15000
rpm
25 ć 12
Figure 6-2 The main interface (D3024)
rpm
4 ć 12
min
min
Figure 6-3 The main interface (D3024R)
Main interface of D3024R model is as figure 6-3. The speed is set to be 15000rpm, temperature of centrifugal
chamber is 4ć, and the running time is 12 minutes. When speed symbol is rotating, indicating the centrifuge is
running. The rotation is faster, the speed is higher. When temperature symbol is blinking, indicating refrigeration is
running, no blinking means refrigeration stop working.
Time symbol displays the ratio of working to time setting. The total time setting is divided into 10 scales.
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P a g e 1 1
USER MANUAL
7. Rotor Preparation
7.1 Prepare the samples
7.2 Inject the samples into tubes
ʽ
CAUTION
Do not overload samples into the centrifuge which will cause leaking.
Do not exceed the actual capacity allowed in the user manual.
7.3 Keep the tubes balanced
Although the centrifuge can accept sample balancing by eye, we recommend that you keep this centrifuge in a
well-balanced condition to extend its life expectancy.
Never intentionally run the centrifuge under an unbalanced condition even thou
exceeded.
gh the allowable imbalance is not
7.4 Inspect the rotor
Check the rotor for corrosion or scratches before using.
ʽ
CAUTION
If any abnormality such as corrosion or scratches are found, stop using the rotor and contact our service center.
Only manufacturer's rotors must be used with the unit.
7.5 Symmetrically load centrifuge tubes to rotor
ʽ
CAUTION
Make sure the rotor lid is securely fixed on the rotor, as well as the rotor and shaft are t
rotor may be moved off while rotating and cause damage to the centrifuge and rotor.
Firmly tighten the rotor lid to the rotor.
ightened. Otherwise, the
Page 12
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High Speed (Refrigerated) Micro-Centrifuge
8. Operation
ʽ
CAUTION
Do not push or lean against the centrifuge while it is running.
Do not run the centrifuge when fragments or sample solutions are left in the centrifuge chamber. Always keep
the centrifugal chamber clean.
If the centrifuge makes strange noise during operation, stop it immediately and contact our service center. Notify
them of the warning code if displayed.
8.1 Normal operation
Turn on the power switch, centrifuge will start self-diagnostic checks, see figure 8-1 below:
HELLO 88 ć 88
Figure 8-1 Self-checking interface
After self-diagnostics, the centrifuge will display the accumulative running time, see figure 8-2 below:
min
00312 56 45
Figure 8-2 Accumulative running time interface
Figure 8-2 indicates the centrifuge has accumulated running time 312 hours 56 minutes and 45 seconds, and then the
centrifuge displays the last running values, see figure 8-3 below:
15000
Speed: 15000rpm; running time: 12minutes; centrifugal chamber temperature: 25
Release the door.
rpm
25 ć 12
Figure 8-3 Last running interface
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min
ć
Page 13
USER MANUAL
8.1.1 Load and replace the rotor
Locking nut
Rotor lid
knob
Load the rotor to the shaft Symmetrically load centrifuge tubes to rotor Load the rotor lid
Figure 8-4 Load the rotor
ʽ
CAUTION
Attach the rotor to the rotor shaft. Ensure the rotor is in position and connected with the shaft, tightening the
locking nut to secure the rotor with shaft, to prevent the rotor damaging the centrifuge.
Ensure the rotor lid is firmly tightened to the rotor.
Load the rotor to shaft to ensure rotor is in position until it connected with the shaft.
You should feel a þclickÿ when the rotor is properly loaded to the shaft. If not, there may be something stuck
between the rotor and the shaft. Double check and clean it if necessary.
Rotate the rotor slightly with your fingers to check if the rotor vibrates. If so attach the rotor again.
Rotate the nut clockwise using the wrench to tighten the rotor to the shaft firmly.
Close the rotor lid, firmly tighten clockwise the lid to the rotor and ensure is in position. Close the door and then
start running.
The method of removing the rotor is as same as the above mentioned by turning the locking nut counterclockwise.
8.1.2 Set the operation programs
Press the button to select required program. The value can be modified when the program is flashing. Rotate the
program button clockwise to increase parameter value. Rotate the program button clockwise to decrease
parameter value. Rotate the program button faster, and the parameter value will increase faster. The minimum
speed increment is 100 rpm, the minimum time increment is 1 second.
(1) Set the speed
Press the select button until the speed rpm is displayed.
When the speed button is selected, the speed symbol will flash the speed value.
The minimum speed value you can set 200rpm, the minimum increment is 10rpm.
Rotate program button clockwise to increase speed value. Rotate the program button anti-clockwise to
decrease speed value.
You can speed-up set the speed value by rotating program button quickly.
There is a circulating function to increase/decrease the speed values. Rotate the program button clockwise to
change settings from small ė large ė maximum ė minimum. Rotate the program button anti-clockwise to
change settings from large ė small ė minimum ė maximum.
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High Speed (Refrigerated) Micro-Centrifuge
(2) Set the time
Press button , time value flashes in the time setting mode.
Rotate the program button to set running time from 30 seconds to 99 minutes.
When the time displays HD, this is a continuous running mode.
(3)Set the temperature (D3024R)
Press select button , until the temperature set flashes for the temperature setting mode.
Rotate the program button , and input tempera
When temperature symbol flashes, this indicates the refrigeration system is running, not flashing indicates the refrigeration
is not running.
8.1.3 Start the operation
(1) Press button to start running
The door must be locked before rotor starts spinning.
Timer will operate once the speed setting value is reached, the screen displays the remaining run time.
ture value range from -20ć to 40ć.
(2) View and modify the operation programs
Operation programs can be modified after the centrifuge reaches the set speed.
Pressing button , returns the display to the program interface and displays setting programs. Press button
to the desired program. When flashing, rotate parameter button to modify values. Release the button after 5
seconds, and the centrifuge will return to normal operation mode and run according to the new value.
If the set time value has been modified, the operation time is not affected and will continue.
(3) Warning display
If an error occurs during the operation, the centrifuge will brake to stop automatically, and display the error code
on the time/display area. The error code can be checked in the table 10-1, and corrective actions can be applied
accordingly.
8.1.4 End the operation
(1) The centrifuge will brake when it reaches the set time or button is pressed.
When the rotor stops rotating, the centrifuge will start beeping to alert the operation has finished.
(2) Open the door
The door can be released automatically when the operation has stopped (D3024).
With the door closed, you are able to press button to open it.
After ending the operation, the program will store the setting values of this operation, and will recall these values
when restarting the program.
(3) Open the door and take out the rotor and samples.
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USER MANUAL
8.2 RCF operation
(1) Turn on the power switch
(2) Set a RCF (Relative Centrifugal Force) value
ʽ
CAUTION
Do not exceed the allowable maximum RCF value of the rotor and adapters.
Press the select button and choose speed unit hg, the speed symbol will flash into RCF value input status.
If no button is pressed after the speed value has flashed after 5 seconds, the input mode will be shut down.
Rotate program button
(3) Set operating conditions
The other operation, please refer to the section 8.1.
to input a RCF value, RCF increment is10hg.
8.3 Pulse operation
This function is used to remove the residual samples adhered to the interior of the tubes or for quick spins.
Note:The button works only while the rotor stopped and the door is locked.
(1) Turn on the power switch and load the rotor to the shaft, tighten the rotor lid and make sure it is in secured
position, and then close the door.
(2) The centrifuge goes into preparation mode and displays last running values. The values can be reset.
(3) Press knob and hold, the centrifuge will speed up to the setting speed. While releasing the knob
during acceleration, the centrifuge will start to decelerate and stop.
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High Speed (Refrigerated) Micro-Centrifuge
9. Maintenance
9.1 Cleaning
ʽ
CAUTION
If do not follow the recommended instructions for cleaning or disinfecting this may damage the centrifuge.
(1) Centrifuge
If the centrifuge is exposed to ultraviolet rays for a long time, the color of the door may be changed or the label
may be peel off. After using, cover the centrifuge with a piece of cloth to protect it from direct exposure.
If the centrifuge needs cleaning, clean it with a
Sterilize the centrifuge by wiping with a cloth moistened with 70% ethanol solution.
(2) Rotor chamber
ʽ
CAUTION
Do not directly pour water, neutral detergent or disinfectant solution into the rotor chamber, otherwise fluids may
leak into the drive units and cause corrosion or deterioration to the bearings.
cloth or sponge moistened with a neutral detergent solution.
If the rotor needs cleaning, clean with cloth or sponge moistened with a neutral detergent solution. Sterilize the
centrifuge by wiping with a cloth moistened with 70% ethanol solution.
(3) Drive shaft
We recommend regular maintenance for drive shaft. You can wipe the drive shaft with soft cloth, and then apply a
thin coat of silicon grease.
(4) Door
Clean and sterilize the door using the same method as the section(1)above.
(5) Rotor
To prevent corrosion, remove the rotor from rotor chamber. If not in use for a long term, then detach the rotor lid
and turn upside down to dry the tube holes and keep clean.
For sample leaks in the rotor, rinse the rotor with water. Apply a thin coat of silicon grease to the rotor when it is
completely dry.
The rotor should be checked every 3 months to ensure the tube and rotor holes keep are clean and apply a thin coat
of silicon grease.
(6) Drain (D3024R)
The centrifuge is equipped with a drain pipe for excess water. Drain off excess water in drain pipe.
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USER MANUAL
9.2 Consumables
Replaceable wearing parts listed below. It is recommended to replace these according this table.
Item Replacement parts
1 Seal ring of door
2 Rubber housing of temperature sensor
3 Seal rings of centrifuge chamber (D3024R)
Replacement conditions
Cracked
9.3 The replacement of seal rings
9.3.1 Instructions
There are three high-temperature rubber seal rings that are fitted to the rotor to achieve a bio-safe condition. The seal
rings may come loose or age after numerous autoclaving and need to be replaced or re-installed.
Seal ring 1 2 3 Seal ring 2 3
Figure 9-1 Seal rings of rotor
9.3.2 Replacement methods
(1) Clean the seal ring slot with neutral detergent solution and ensure its dry.
(2) Evenly coat with glue (501) in the seal ring slot and place seal ring into slot, press evenly to make it evenly
contact to the slot bottom and bond firmly.
(3) Leave for 20 minutes for the glue to completely solidify and set.
9.4 Routine inspection
(1) Check the centrifuge is on a firm, flat and level surface, ensure four feet are seated on the surface firmly.
(2) Check the centrifuge is grounded properly. Use a multi-meter to check if there is a short circuit between the power
cord grounding pin and the motor shaft. If an open circuit is found, check for reasons and troubleshooting before use.
Page 18
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High Speed (Refrigerated) Micro-Centrifuge
10. Troubleshooting
10.1 Possible problems and solutions
This centrifuge has a self-diagnostic function. If a problem occurs, an error/warning code will be displayed on the
time display screen, and the operator can determine the malfunction with the warning code below.
Symptom Causes Solutions
Nothing appears on the screen when
the power is turned on.
E-02
Door fault
Error code
appeared on the
time display
screen
E-04
Temperature fau
E-06
Set wrong value
E-1086 Read the service manual. Contact the service center.
lt
Facility power circuit breaker tripped.
The fuse has blown.
• The door opened while running.
• Press the button with the door
opened.
• The air vents are blocked.
• Radiator fan is damaged.
• The setting value exceeds the
allowable range.
Correct and turn on the power.
Replace the fuse.
Close the door immediately.
• Close the door,and then start to
operate.
• Clean air vents.
• Replace radiator fan.
• Modify the set value.
Table 10-1 Possible problems and solutions
Warning codes E-1E-9 are related to incorrect operation/programming. You can continue running the centrifuge
after implementing corrective procedures.
10.2 How to open the door
10.2.1 In the case of power on
ʽ
CAUTION
The door just can be opened while the power is on and rotor stops rotating.
(1) Turn on the power switch, release the door automatically.
(2) The door will be released automatically once the operation is finished.
(3) It is available to release the door by pressing button once the rotor stops
10.2.2 In the case of power outage
The door cannot be opened automatically if there is a power outage. It is available to be opened manually as follows.
(1) Ensure if the rotor has stopped rotating.
Listen carefu
(2) Insert a screw driver into a hole to open door.
Holes are located on the left and right sides of the unit.
Insert a screw driver into the two holes and push forward to release the door.
lly to ensure no rotating sound can be heard.
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Page 19
USER MANUAL
10.3 Replacement of fuses
(1) There are two fuses of D3024, 250V, 5A time-delay type, size: Ï5h20. There are two fuses of D3024R, 250V, 10A
time-delay type, size: Ï5h20.
(2) The fuse holder is located in the power inlet. Pull out the fuse holder from power inlet and replace the fuses if
necessary.
11. Instructions for the Rotor and Tubes
ʽ
CAUTION
Read the instructions thoroughly, to properly load and use rotor.
Do not exceed the allowable maximum speed of rotorǃtube and adapters etc. Ensure the allowable maximum
speed of adapters is lower than the rotorÿs maximum speed.
11.1 Rotor instructions
11.1.1 Rotor structure
Knob Locking nut Rotor lid
Page 20
Seal rings Shaft Tube holes
Figure 11-1 The rotor structure
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High Speed (Refrigerated) Micro-Centrifuge
11.1.2 Available rotors and adapters
All rotors are bio-safe when the rotor lid is tightened to the rotor and if centrifuge tubes are enclosed into rotor to
ensure the sample does not leak in centrifugal process. If a rotor lid is not available, the rotor is not in a bio-safe
condition. The rotors can be used as follows:
Rotor type
1 AS24-2
2 AS36-05
3 AS4-PCR8
11.1.3 Notice
(1) The centrifuge rotor can separate samples with a density lower than 2.0 g/ml. I
ml, please calculate allowable speed depending on the following formula.
Allow Speed (rpm) = Maximum speed h (2.0(g/ml) /Sample density (g/ml))1/2
ID
code
Tubes Adapters
2/1.5ml tube 15000 8.5 21380
0.2ml PCR tube A02P2 15000 6.9 17350
0.5ml micro
tube
0.5ml micro
tube
PCR8 serial
tube
PCR8 serial
tube
Table 11.1 Rotors and adapters
A05P2 15000 7.6 19100
A02P05 15000 7.6 19100
Maximum
speed
(rpm)
15000 8.5 21380
Maximum
centrifugal
radius
rmax (cm)
6.5/7.2 16350/18100
f the samples density is over 2.0 g/
Maximum RCF
Rcf (hg)
(2) To prevent corrosion, remove the rotor from rotor chamber if not in use for a long term, then detach the rotor lid
and place upside down to dry the tube holes.
(3) If samples have leaked in the rotor holes, wash th
surface after drying.
(4) It is necessary for a regular rotor maintenance and should be cleaned every 3 months to keep the tube holes and
shaft clean. Apply a thin coat of silicon grease.
11.1.4 Autoclaving
The rotor is manufactured in high-strength aluminum alloy material and can be autoclaved: 121ć˄1.0kg/cm2˅20
minutes.
11.1.5 Bio-safe seal ring
The rotor is sealed with
autoclaving and may need to be replaced or re-installed. For replacement methods please refer to the section 9.3.
three bio-safe high-temperature sealing rings. The seal rings wear or age after numerous
e hole with water, apply a thin coat of silicon grease on the rotor
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USER MANUAL
11.2 Tubes
11.2.1 Cleaning and sterilizing tubes
Conditions
Cleaning
Sterilization
PA: Polyallomer PC: Polycarbonate PP: Polypropylene
Cleaning fluids
Ultrasonic cleaning Neutral detergent(pH7) O O O
Autoclaving
Boiling 15 to 30 minutes O O O
Ultraviolet sterilization 200-300nm X X X
Gas sterilizati
on
Materials
Acidic(pH5 or lower) X X X
Acidic(higher than pH5 ) O O O
Alkaline(higher than pH9 ) O X O
Alkaline(pH9 or lower) O O O
Neutral(pH7) O O O
Warm water(up to 70ć) OOO
115ć(0.7kg/cm2)30minutes O O O
121ć(1.0kg/cm2)20 minutes X O O
126ć(1.4kg/cm2)15 minutes X X X
Ethylene oxide O X O
Formaldehyde O O O
PA PC PP
11.2.2 Cleaning PC tubes
PC material is low in chemical resistance against alkaline solutions. Avoid using neutral detergents with pH
higher than 9. Note that pH of some neutral detergents are still higher than 9 even if diluted according to the
manufacturerÿs instructions. Use detergent with its pH between 7 and 9.
11.2.3 Autoclaving P AǃPC and PP tubes
PA begins softening at about 12
minutes, PC and PP tubes at 121ć˄0.1kg/cm2˅for 20 minutes. If a certain temperature is exceeded, the tubes may
be deformed.
When use a sterilizing chamber, please operate as follows:
˄1˅Place tubes in vertical position, mouths upward. If tubes are placed sideways, they may deform into an oval
shape due to gravity.
˄2˅Remove locking nut and lid to prevent from deformation or rupture.
˄3˅Wait until the sterilizing chamber cools down to the room temperature before removing tubes.
0ć, PC and PP at about 130ć. Autoclave PA tubes at 115ć˄0.7kg/cm2˅for 30
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High Speed (Refrigerated) Micro-Centrifuge
11.2.4 Conditions and life expectancy of tubes
The life expectancy of plastic tubes depends on the characteristics of samples, speed of the rotor used, temperature
applied and so on. When the plastic tubes are used for ordinary aqueous samples (pH between 5.0 and 9.0),
expectancies are defined as follows.
Be operated at the maximum speed:
High quality tubes (PAǃPCǃPP): 30-50 operations
Ordinary tubes(PAǃPCǃPP): around 10 operations˄Using in low speed can extend the tube life˅.
Life expectancy of tubes also depends on the pretreatment conditions such as cleaning and sterilization, lifetime can
be cut down.
Notice: Do not use damaged or cracked tubes.
their life
12. Calculate RCF
Relative Centrifuge Force (RCF) can be determined with the following calculation formula.
RCF = 1.118 h r h n2 h 10-5
R ü rotating radius unit: cm; n ü rotating speed, unit: rpm
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USER MANUAL
13. Ordering information
Cat. No. Model Descriptions
912015139999 D3024
912115129999 D3024
912215129999 D3024
912315129999 D3024
922015139999 D3024R
922115129999 D3024R
922215129999 D3024R
922315129999 D3024R
Accessories
19400006 AS24-2
19400007 AS36-05
19400008 AS4-PCR8
High Speed Micro-Centrifugeˈwith AS24-2 rotor kits, US plug,
110V/50Hz/60Hz
High Speed Micro-Centrifugeˈwith AS24-2 rotor kits, Cn plug,
220V/50Hz/60Hz
High Speed Micro-Centrifugeˈwith AS24-2 rotor kits, Euro plug ,
220V/50Hz/60Hz
High Speed Micro-Centrifugeˈwith AS24-2 rotor kits, UK plug,
220V/50Hz/60Hz
High Speed Refrigerated Micro-Centrifugeˈwith AS24-2 rotor kits, US plug,
110V/50Hz/60Hz
High Speed Refrigerated Micro-Centrifugeˈwith AS24-2 rotor kits, Cn plug,
220V/50Hz/60Hz
High Speed Refrigerated Micro-Centrifugeˈwith AS24-2 rotor kits, Euro
plug, 220V/50Hz/60Hz
High Speed Refrigerated Micro-Centrifugeˈwith AS24-2 rotor kits, UK
plug, 220V/50Hz/60Hz
Rotor kits with cover, fixing clips and O' ring, 15000rpm, 2ml*24, used with
D3024 & D3024R
Rotor kits with cover, fixing clips and O' ring, 15000rpm, 0.5ml*36, used with
D3024 & D3024R
Rotor kits with cover, fixing clips and O' ring, 15000rpm, 4-PCR8, used with
D3024 & D3024R
19500001 A02P2 0.2ml rotor adapter, used with A12-2 & AS24-2 rotorsˈ24pcs/pk
19500002 A05P2 0.5ml rotor adapter, used with A12-2 & AS24-2 rotorsˈ24pcs/pk
19500003 A02P05 0.2ml rotor adapter, used with AS36-05 rotorˈ36pcs/pk
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High Speed (Refrigerated) Micro-Centrifuge
14. Warranty
14.1 Warranty of centrifuge
This centrifuge is guaranteed for two years from the date of delivery provided that it has been operated and
maintained properly.
14.2 Warranty of the rotor
The rotor is guaranteed for 5 years from the date of delivery upon manufacturer. Please pay attention, do not use the
rotor once it has been corrosion or fatigue damage. The warranties of the centrifuge and the rotor become invalid in
the case of the following conditions even if within the guarantee period expires:
˄1˅Failures caused by incorrect installation.
˄2˅Failures caused by rough or improper handling.
˄3˅Failures caused by conveyance or relocation after installation.
˄4˅Failures caused by unauthorized disassembly or modification.
˄5˅Failures caused by using non-standard spare parts or accessories and unauthorized modification of the rotor or
centrifuge.
˄6˅Failures caused by natural disasters including fire, earthquakes and so on.
˄7˅Consumables and parts have a limited guarantee period.
15. After-sales Service
In order to ensure to operate centrifuge safely and efficiently, it is necessary for regular maintenance. If centrifuge
has problems, do not attempt to repair it by yourself. Contact our sales or service center.
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Page 25
SCILOGEX, LLC
1275 Cromwell Ave.
Suite C6
Rocky Hill, CT 06067
USA
Tel:1- (860) 436-9221
Fax:1- (860) 436-9745
info@scilogex.com
www.scilogex.com
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