Sartorius Vivaspin 500 Operating Instructions Manual
85030-511-58
Technical data and operating instructions
Vivaspin® 500 µl and 2 ml
Vivaspin® 500 and 2 10K devices for in vitro diagnostic use
Vivaspin
®
500 and 2 3K, 5K, 30K, 50K, 100K, 300K, 1000K and 0.2 µm
devices for research use only; not for use in diagnostic procedures
For French, Italian, and Spanish manuals, please go to
www.sartorius.com/en/product-family/product-family-detail/m-centrisart-i/
2 |
Vivaspin® 500 µl and 2 ml – Introduction
Storage conditions|shelf life
Vivaspin
®
ultrafiltration spin columns
should be stored at 15 – 30°C. The devices
should be used before the expiry date
printed on the box.
Introduction
Vivaspin
®
Concentrators are disposable
ultrafiltration devices for the concentration
of biological samples. Vivaspin
®
500 is suitable for sample volumes of 100–500 µl and
the Vivaspin
®
2 can handle samples up to
2 ml. Vivaspin
®
2 can effectively be used in
either swing bucket or fixed angle rotor
accepting 15 ml centrifuge tubes.
The patented vertical membrane design
and thin channel filtration chamber
(US 5,647,990) minimises membrane fouling
and provides high speed concentrations,
even with particle laden solutions.
Vivaspin
®
500 can be used in a benchtop
fixed angle rotor, accepting 2.2 ml
centrifuge tubes.
C
The Vivaspin® 500 & 2 product line includes
9 different cutoffs (Molecular Weight Cutoff,
MWCO):
– Vivaspin
®
500 & 2 3K device: 3,000 MWCO
– Vivaspin
®
500 & 2 5K device: 5,000 MWCO
– Vivaspin
®
500 & 2 10K device:
10,000 MWCO
– Vivaspin
®
500 & 2 30K device:
30,000 MWCO
– Vivaspin
®
500 & 2 50K device:
50,000 MWCO
– Vivaspin
®
500 & 2 100K device:
100,000 MWCO
– Vivaspin
®
500 & 2 100K device:
300,000 MWCO
– Vivaspin
®
500 & 2 100K device:
1000,000 MWCO
– Vivaspin
®
500 & 2 100K device: 0.2 µm
Vivaspin
®
500 & 2 10K filter devices are for
in vitro diagnostic use and can be used to
concentrate serum, urine, cerebrospinal
fluid, and other body fluids prior to analysis.
Vivaspin
®
500 & 2 3K, 5K, 30K, 50K, 100K,
300K, 1.000K and 0.2 µm filter devices are
for research use only and not for use in
diagnostic procedures. The Vivaspin
®
500 &
2 devices are supplied non-sterile and are for
single use only.
| 3
Vivaspin® 2
The Vivaspin
®
2 is specifically designed with
low internal surface and membrane area in
order to achieve superior recoveries from
very dilute solutions.
Another feature of the Vivaspin
®
2 is the
choice of directly pipetting the concentrate
from the dead stop pocket built into the
bottom of the concentrator, or alternatively
reverse spinning the concentrate into the
recovery cap.
Membrane Alternatives
In addition to the proven high flux
polyethersulfone (PES) membrane range
which is recommended with most solutions,
Vivaspin
®
2 is additionally offered with
cellulose triacetate (CTA) and Hydrosart
®
.
CTA is particularly recommended when
high recovery of the filtrate solution is of
primary importance. Hydrosart
®
is a stabilised
cellulose based membrane that has been
optimised for the biotechnological industry.
The Hydrosart
®
membrane is a stable polymer
that features a broad pH range. Hydrosart
®
is also extremely hydrophilic, making it
non-protein binding, virtually non-foul,
and has extremely high flux. Hydrosart
®
is
available in 5k, 10k, and 30k molecular
weight cutoffs.
Please note that membrane behaviour largely
depends on the specific characteristics of the
solution being processed. Sartorius Stedim
Biotech recommends that users experiment
with alternative membranes in seeking to
optimise their process performance.
Equipment Required
1. Centrifuge with swing bucket of fixed
angle (minimum 25°) rotor.
Device Carrier Required
Vivaspin
®
500 2.2 ml/11 mm d
Vivaspin
®
2 15 ml/17 mm d
2. Pipettes for sample delivery and removal.
For maximum recovery a thin gel loader type
is recommended.
4 |
Operation
1. Select the most appropriate membrane
for your sample. For maximum recovery
select a MWCO at least 50% smaller than
the molecular size of the species of interest.
2. Fill concentrator with up to maximum
volumes shown in table 1. (Ensure lid is fully
seated).
3. Insert assembled concentrator into
centrifuge (when fixed angle rotors are
used, angle concentrator so that the
printed window faces upwards|outwards).
4. Centrifuge at speeds recommended
in table 2, taking care not to exceed the
maximum g force indicated by membrane
type and MWCO.
5. Once the desired concentration is
achieved, (see tables 3a & 3b for guide
to concentration times), remove assembly
and recover sample from the bottom of
the concentrate pocket with a pipette.
Removing the Vivaspin
®
2 body from
the filtrate tube
The sleeve (seen from the end) is oval in
cross section. The tube is round in cross
section to give a tight fit to the sleeve. To
release the tube from the sleeve, you must
pinch the tube – to press it into an oval
shape – before removing it with a twisting
action.
Press
here
Press
here
Tube Sleeve
Reverse spin with Vivaspin® 2
Depending on user preference and need
for sample storage, the concentrate can be
reverse spun into the concentrate recovery
cap (when fixed angle rotors are used, angle
concentrator so that the printed window
faces upwards|outwards). In this procedure
remove filtrate tube, invert the concentrator
body, insert concentrate recovery cap into
filtrate tube and then spin at up to 3,000 g
for 2 minutes.
Desalting|Buffer Exchange
1. Concentrate sample to desired level.
2. Empty filtrate container.
3. Refill concentrator with an appropriate
solvent.
4. Concentrate the sample again and repeat
the process until the concentration of
contaminating microsolute is sufficiently
reduced. Typically 3 wash cycles will remove
99% of initial salt content.
| 5
Vivaspin® 2 Reverse Spin
Filtrate and
concentrate
can be sealed
for storage.
Concentrate
recovery cap
Concentrate
Position with printed
window upwards|outwards
Concentrator body
Filtrate tube
Filtrate
Equipment required Vivaspin® 500 Vivaspin® 2
Centrifuge
Rotor type Fixed angle Swing bucket or Fixed angle
Minimum rotor angle 40° 25°
Rotor cavity To fit 2.2 ml (11 mm) To fit 15 ml (17 mm)
conical bottom tubes conical bottom tubes
Concentrate recovery
Pipette type Fixed or variable volume Fixed or variable volume
Recommended tip Thin gel loader type Thin gel loader type
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