Technical data and operating instructions
Vivaspin® 4
Vivaspin® 4 10K device for in vitro diagnostic use
85030-516-16
Vivaspin® 4 ml – Introduction
Storage conditions|shelf life
Vivaspin
®
4 ultrafiltration spin columns
should be stored at 15 – 30°C. The devices
should be used before the expiry date
printed on the box.
Introduction
Vivaspin
®
4 ml concentrators are disposable
ultrafiltration devices for the concentration
of biological samples. Maximum initial
sample volumes range from 1 ml to 4 ml.
They can be effectively used in either swing
bucket or fixed angle rotors accepting
15 ml conical bottom centrifuge tubes.
The patented vertical membrane design
and thin channel filtration chamber (US
5,647,990, second patent pending) minimises membrane fouling and provides high
speed concentrations, even with particle
laden solutions. Vivaspin
®
4 is available with
the high flux polyethersulfone membrane
range which is recommended for most
solutions.
The Vivaspin
®
4 product line
includes 1 cutoffs (Molecular Weight Cutoff,
MWCO):
– Vivaspin
Vivaspin
®
4 10K device: 10,000 MWCO
®
4 10K filter devices are for in vitro
diagnostic use and can be used to concentrate serum, urine, cerebrospinal fluid, and
other body fluids prior to analysis. The
Vivaspin
®
4 devices are supplied non-sterile
and are for single use only.
Operation
1. Select the most appropriate membrane
for your sample. For maximum recovery
select a MWCO at least 50% smaller than
the molecular size of the species of interest.
2. Fill concentrator with up to maximum
volume shown in table 1. (Ensure lid is fully
seated).
3. Insert assembled concentrator into
centrifuge (when fixed angle rotors are
used, angle concentrator so that the
printed window faces upwards | outwards).
4. Centrifuge at speeds recommended
in table 2 taking care not to exceed the
maximum g force indicated by membrane
type and MWCO.
5. Once the desired concentration is
achieved, (see tables 3a and 3b, for a guide
to concentration times), remove assembly
and recover sample from the bottom of
the concentrate pocket with a pipette.
The filtrate tube can be sealed for storage.
Desalting | Buffer exchange
1. Concentrate sample to desired level or
at least 5+.
2. Empty filtrate container.
3. Refill concentrator with an appropriate
solvent.
4. Concentrate the sample again and repeat
the process until the concentration of
contaminating microsolute is sufficiently
reduced. Typically 3 wash cycles will remove
99% of initial salt content.
Equipment required Vivaspin
®
4
Centrifuge
Rotor type Swing bucket or
Fixed angle
Minimum rotor angle 25°
Rotor cavity To fit 15 ml (17 mm)
conical bottom tubes
Concentrate recovery
Pipette type Fixed or variable
volume
Recommended tip Thin gel loader type
2 |
Technical Specifications
Technical specifications
Vivaspin
®
4
Concentrator capacity
Swing bucket rotor 4 ml
Fixed angle rotor 4 ml
Dimensions
Total length 122 mm
Width 17 mm
Active membrane area 2.0 cm
2
Hold up volume of <10 µl
membrane
Dead stop volume* 20 µl
Materials of construction
Body Polycarbonate
Filtrate vessel Polypropylene
Concentrator cap Polycarbonate
Membrane Polyethersulfone
Recommended spin speed (xg)
Vivaspin
®
4
Membrane Fixed Swing
angle bucket
10K PES 10,000 4,000
* Dead stop volume as designed in moulding
tool. This volume may vary depending on
sample, sample concentration, operation
temperature and centrifuge rotor.
Usage Tips
4. Chemical Compatibility
Vivaspin
®
concentrators are designed for use
with biological fluids and aqueous solutions.
For chemical compatibility details, refer to
table 4.
1. Flow Rate
Filtration rate is affected by several parameters, including MWCO, porosity, sample concentration, viscosity, centrifugal force and
temperature. Expect significantly longer spin
times for starting solutions with over 5% solids. When operating at 4°C flow rates are
approximately 1.5 times slower than at 25°C.
Viscous solutions such as 50% glycerine will
take up to 5 times longer to concentrate
than samples in a predominantly buffer
solution.
2. Pre-rinsing
Membranes fitted to Vivaspin
®
concentrators
contain trace amounts of Glycerine and
Sodium azide. Should these interfere with
analysis they can be removed by rinsing fill
volume of buffer solution or deionised water
through the concentrator. Decant filtrate
and concentrate before processing sample
solution. If you do not want to use the
pre-rinsed device immediately, store it in the
refrigerator with buffer or water covering
the membrane surface. Please do not allow
the membrane to dry out.
3. Sterilisation
Polyethersulfone membranes should not
be autoclaved as high temperatures will
substantially increase membrane MWCO.
To sterilise, use a 70% ethanol solution or
sterilising gas mixture.
4. Chemical Compatibility
Vivaspin
®
concentrators are designed for use
with biological fluids and aqueous solutions.
Refer to the chemical compatibility table on
this datasheet.
| 3
Performance Characteristics
Table 3: Performance Characteristics Vivaspin® 4
Time to concentrate up Concentrate
to 30x [min.] at 20°C recovery %
Start volume 4 ml 4 ml
BSA 1.0 mg/ml (66,000 MW)
5,000 MWCO PES 15 96%
10,000 MWCO PES 10 96%
30,000 MWCO PES 10 95%
IgG 0.25 mg/ml (160,000 MW)
30,000 MWCO PES 10 95%
50,000 MWCO PES 10 95%
100,000 MWCO PES 10 95%
4 |
Chemical Compatibility
Table 4: Chemical Compatibility
Vivaspin
Solutions PES
Compatible pH range pH 1–9
Acetic Acid (25.0%) OK
Acetone (10.0%) NO
Acetonitrile (10.0%) NO
Ammonium Hydroxide (5.0%) ?
Ammonium Sulphate (saturated) OK
Benzene (100%) NO
n-Butanol (70%) ?
Chloroform (1.0%) NO
Dimethyl Formamide (10.0%) ?
Dimethyl Sulfoxide (5.0%) NO
Ethanol (70.0%) OK
Ethyl Acetate (100%) NO
Formaldehyde (30%) OK
Formic Acid (5.0%) OK
Glycerine (70%) OK
Guanidine HCI (6 M) OK
Hydrocarbons, aromatic NO
Hydrocarbons, chlorinated NO
Hydrochloric Acid (1 M) OK
Imidazole (300 mM, max 2 hrs) OK
Isopropanol (70%) OK
Lactic Acid (5.0%) OK
Mercaptoethanol (10 mM) OK
Methanol (60%) OK
Nitric Acid (10.0%) OK
Phenol (1.0%) NO
Phosphate Buffer (1.0 M) OK
Polyethylene Glycol (10%) OK
Pyridine (100%) NO
Propanol (70%) OK
Sodium Carbonate (20%) NO
Sodium Deoxycholate (5.0%) OK
®
4
| 5
Vivaspin® 4
Solutions PES
Compatible pH range pH 1–9
Sodium Dodecylsulfate (0.1 M) OK
Sodium Hydroxide (2.5 M) NO
Sodium Hypochlorite (200 ppm) NO
Sodium Nitrate (1.0%) OK
Sulfamic Acid (5.0%) OK
Tetrahydrofuran (5.0%) NO
Toluene (1.0%) NO
Trifluoroacetic Acid (10%) OK
Tween 20 (0.1%) OK
Triton X-100 (0.1%) OK
Urea (8 M) OK
OK = Acceptable ? = Questionable NO = Not recommended
In Vitro Diagnostic Product Labeling
The following tabe defines the symbols found on Vivaspin
®
4 10K device labels.
Symbol SymbolDefinition Definition
In vitro diagnostic medical device
Catalogue number
Do not reuse
Use by
Batch code
Date of manufacture
Manufacturer
Temperature limitation
Non-sterile product
CE conformity marking
6 |
Ordering Information
Vivaspin® 4 Polyethersulfone Qty per box Prod. no.
10,000 MWCO 25 VS0403
10,000 MWCO 100 VS0404
| 7
Sartorius Stedim Lab Ltd
Sperry Way
Stonehouse Park
Gloucestershire
GL10 3UT, UK
www.sartorius.com
Copyright by
Sartorius Lab Instruments
GmbH & Co. KG, Goettingen,
Germany.
All rights reserved. No part
of this publication may
be reprinted or translated in
any form or by any means
without the prior written
permission of Sartorius Lab
Instruments GmbH & Co. KG.
The status of the information,
specifications and illustrations
in this manual is indicated
by the date given below.
Sartorius Lab Instruments
GmbH & Co. KG reserves the
right to make changes to the
technology, features,
specifications and design of the
equipment without notice.
Status:
June 2016,
Sartorius Lab Instruments
GmbH & Co. KG,
Goettingen, Germany
Printed in the EU on paper bleached
without chlorine.
Publication No.: SLU2004-e160608
Ver. 06 | 2016
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