85034-538-74
Technical data and operating instructions
Vivacon® 500
For in vitro use only
2 |
Vivacon® 500 µl – Introduction Operation
Storage conditions | shelf life
Vivacon® ultrafiltration spin columns
should be stored at room temperature.
The devices should be used before the
expiry date printed on the box.
Introduction
Vivacon
®
500 concentrators are disposable
ultrafiltration devices optimally suited for
DNA and protein concentration. For optimal
performance with DNA and protein samples,
they are equipped with the patented regenerated cellulose membrane Hydrosart
®
.
Vivacon
®
500 can be used in a benchtop
fixed angle rotor, accepting 1.5 | 2.2 ml
centrifuge tubes.
Equipment required for
Vivacon
®
500
Centrifuge
Rotor type Fixed angle
Minimum 40°
rotor angle
Rotor cavity To fit 1.5 | 2.2 ml/11
mm
conical bottom tubes
1. When working with DNA samples, select
a molecular weight cut off (MWCO) which
retains the fragment size of double
stranded DNA (ds DNA) as shown in
Table 5. When working with proteins,
select a MWCO at least 50% smaller than
the molecular size of the protein of
interest.
2. Fill concentrator with up to maximum
volumes shown in Table 1. (Ensure lid
is fully sealed).
3. Insert assembled concentrator into
centrifuge.
4. Centrifuge at speeds recommended in
Table 2, taking care not to exceed the
maximum g force indicated by the MWCO.
5. Once the desired concentration is
achieved, (see Table 3 or 4 for guide to
concentration times), remove assembly
and recover sample by reverse spinning
the concentrate into a fresh collection
tube. In this procedure remove filtrate
tube, invert the concentrator body into
new filtrate tube and then spin at up
to 2,500 g for 2 minutes (or pulse for
20–30 seconds).
The filtrate can be sealed for storage by
closing the filtrate tube cap.
Note:
It is not possible to do a backflush
with the device.
Table 1: Technical Specifications
Concentrator capacity
Fixed angle rotor 0.5 ml
Dimensions
Total length 45 mm
(Concentration)
Total length (Back-spin) 47.5 mm
Width 12.4 mm
Active membrane area 0.32 cm
2
Hold-up volume < 5 µl
Dead stop volume 5 µl (40° rotor)
Materials of construction
Body Polycarbonate
Filtrate vessel Polypropylene
Membrane
2kDa - 100 kDa Hydrosart
®
125 kDa Cellulose
Acetate (CA)
O-ring Medical grade
silicone
Table 2: Recommended Spin Speed in
Fixed Angle Rotor (x g)
Membrane cut off For DNA For proteins
2 kDa MWCO 7,500 14,000
10 kDa MWCO 7,500 14,000
30 kDa MWCO 5,000 14,000
50 kDa MWCO 5,000 14,000
100 kDa MWCO 3,000 8,000
125 kDa MWCO 2,500* 5,000
Technical Specifications
Desalting | Buffer Exchange
1. Concentrate sample to desired level.
2. Empty filtrate container.
3. Refill concentrator with an appropriate
solvent.
4. Concentrate the sample again and repeat
the process until the concentration of
contaminating microsolute is sufficiently
reduced. Typically 3X wash cycles will
remove 99% of initial salt concentration.
Vivacon
®
500 Reverse Spinning
| 3
Hydrosart
membrane
Reverse
spinning
concentrate
into fresh
collection
tube
Collection tube
fits standard
1.5 | 2.2 ml tube
carriers
Filtrate can
be sealed
for storage
* Spin speed 2,500 + g for DNA samples > 900 bp when using a 125 kDa MWCO.
For DNA samples >650 bp, spin at 1,000 + g.