Sartorius Tacta User Manual
User Manual
Tacta® Mechanical Pipette
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Table of Contents
1. Introduction ................................................................................................................................................................................................................................................... 3
1.1 Intended Use .............................................................................................................................................................................................................................................. 3
1.2 Pipette Parts and Materials ........................................................................................................................................................................................................... 3
1.3 Sartorius Pipette Tips ...................................................................................................................................................................................................................... 4
1.4 Adjustment Tool ................................................................................................................................................................................................................................ 4
2. Package Contents ......................................................................................................................................................................................................................................... 4
3. Tagging the Pipette ..................................................................................................................................................................................................................................... 5
4. Stands and Holders ...................................................................................................................................................................................................................................... 5
4.1 Installing the Pipette Holder ......................................................................................................................................................................................................... 5
4.2 Using the Carousel Stand Adapter ............................................................................................................................................................................................. 6
5. Preparing the Pipette .................................................................................................................................................................................................................................. 6
5.1 Setting the Volume .......................................................................................................................................................................................................................... 6
5.2 Attaching and Ejecting Tips .......................................................................................................................................................................................................... 7
5.3 Using Protective Tip Cone Filters .............................................................................................................................................................................................. 10
6. Operating the Pipette ............................................................................................................................................................................................................................... 11
6.1 Good Pipetting Practices ............................................................................................................................................................................................................. 11
6.2 Forward Pipetting .......................................................................................................................................................................................................................... 11
6.3 Reverse Pipetting ...........................................................................................................................................................................................................................12
6.4 Repetitive Reverse Pipetting ......................................................................................................................................................................................................12
7. Maintaining the Pipette ............................................................................................................................................................................................................................. 12
7.1 Cleaning the Outer Surface of the Pipette (daily) ................................................................................................................................................................13
7.2 Cleaning the Lower Part of the Pipette (every three months) .........................................................................................................................................13
7.3 Sterilizing the Pipette ................................................................................................................................................................................................................... 15
8. Testing and Adjusting the Pipette .........................................................................................................................................................................................................15
8.1 Testing Pipette Performance ......................................................................................................................................................................................................16
8.2 Adjusting the Pipette ................................................................................................................................................................................................................... 17
9. Troubleshooting ..........................................................................................................................................................................................................................................18
10. Order Information ....................................................................................................................................................................................................................................19
11. Warranty Information .............................................................................................................................................................................................................................20
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1. Introduction
1.1 Intended Use
The Tacta® pipette is intended, designed and manufactured for dispensing liquids in a variety of
applications, and to be used in combination with Sartorius Optifit Tips or SafetySpace Filter Tips.
The Sartorius pipette and tip combination, fall within the scope of in-vitro diagnostics, and can
be used as a diagnostic medical device in related applications. Thereby, Tacta® and Sartorius tips
fulfil the relevant demands of the Directive 98/97/EC of the European Parliament.
The Tacta® product range covers a volume range of 0.1 μl to 10 ml. It is recommended that Sartorius Optifit Tips or SafetySpace Filter
Tips are used with Sartorius pipettes to ensure optimum compatibility and performance.
The Sartorius pipette is a general purpose laboratory device that fulfils ISO 9001 and ISO 13485 standards.
Read this user manual carefully before using the pipette for the first time. Additional copies can be downloaded from www.sartorius.
com or hardcopies ordered by email from lhinfo.finland@sartorius.com.
NOTE: Prolonged pipetting can cause Work Related Upper Limb Disorder (WRULD). The manufacturer is not responsible for WRULD or
any related injuries caused by using a pipette.
1.2 Pipette Parts and Materials
1. Operating button (polyamide (PA), silicone (SI), stainless steel (SS))
2. Finger support (polypropylene (PP))
3. Tip ejector (PA)
4. Handle (PP)
5. Volume lock (SI)
6. Display (polycarbonate (PC))
7. Tip ejector collar (PP)
8. Tip cone (polyvinylidenefluoride (PVDF) in 3, 10, 20, and 100 μl pipettes, polyetherimide (PEI) in 200 and 300 μl pipettes,
polyphenylenesulphide (PPS) in 1000 μl, 5000 μl, and 10 ml pipettes)
9. Safe-Cone Filter (polyethylene (PE))
10. Adjustment window (PC)
11. Tip cone housing (PA)
12. Tip ejector bar (PA)
13. Stainless steel, corrosion-resistant metal ring (SSt EN 1.4404 / AISI 316L)
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SafetySpace
air gap
]
1.3 Sartorius Pipette Tips
We recommend only using Sartorius pipette tips with the Tacta® pipette. Using fully
compatible tips guarantees the pipette’s performance specifications and ensures pipetting
accuracy and precision.
Sartorius tips are made of pure virgin polypropylene and manufactured in protected
cleanroom conditions.
Sartorius non-filter tips are available in trays, bulk packages, and space-saving refill systems.
Both non-filter tips and trays are autoclavable at 121°C (252°F), 20 min, 1 bar (15 psi). All
Single Tray racks and clean Refill Packs are certified RNase, DNase, and endotoxin-free.
To avoid aerosol contamination, use SafetySpace Filter Tips and discard them after each
pipetting. These can also be used to avoid sample loss caused by the sample coming into
contact with the filter. The extra space between the sample and the filter (see picture)
ensures that even foaming or viscous liquids do not come into contact with the filter in
reverse pipetting or repetitive/multiple dispensing modes.
For more information, please visit www.sartorius.com or contact your local Sartorius
representative.
2. Package Contents
The Tacta® pipette package contains the following items:
− Tacta® mechanical pipette
− Sartorius Optifit tip(s)
− Adjustment tool
− Four identification tags
− Safe-Cone Filters (except 3 and 10 μl models)
− Autoclavable grease, 1 g
− Pipette holder
− Carousel stand adapter
− QC certificate from gravimetric testing according to ISO 8655-6 standard
− Quick Start Guide
If any item in the package is missing or damaged, contact your local Sartorius
representative.
1.4 Adjustment Tool
For situations where factory calibration is not applicable, the adjustment tool allows you to
calibrate the pipette accurately.
NOTE: For more information on adjusting of the Tacta® pipette see 8.2, Adjusting the pipette.
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3. Tagging the Pipette
You can use personalized tags to make each Tacta® pipette easily identifiable.
NOTE: If you intend to autoclave the pipette, make sure you use suitably resistant ink.
1. Write the identifying information on the tag.
2. Attach the tag to the finger support.
4. Stands and Holders
For convenience and safety, always keep the pipette upright in its holder or stand when not in use.
4.1 Installing the Pipette Holder
1. Clean the shelf surface with ethanol.
2. Remove the protective paper from the adhesive tape.
3. Install the holder by pressing it against the edge of the shelf.
4. Hook the finger support onto the holder.
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4.2 Using the Carousel Stand Adapter
The Carousel Stand (Order Code 725600) is made compatible with Tacta® by using the adapter provided in the Tacta® package.
5. Preparing the Pipette
5.1 Setting the Volume
The volume to be aspirated is shown on the display. To adjust the volume, you can use the volume lock button in two different ways.
We recommend that in normal use the volume lock is engaged, with the red bar hidden. This prevents accidental volume changes.
To adjust the volume:
1. Press and hold the volume lock button.
2. Turn the operating button to adjust the volume.
3. Release the button to lock the volume adjustment again.
OR
1. Slide the volume lock button up. The red bar becomes visible and volume adjustment is now unlocked.
2. Turn the operating button to adjust the volume.
NOTE: A Carousel Stand that holds six Tacta® pipettes is available separately see 10.1 Order Information and Specifications.
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!
Always lock after setting the volume
5.2 Attaching and Ejecting Tips
The pipette has a spring-loaded tip cones for optimal sealing.
To attach a tip:
1. Make sure the pipette tip cones are clean and undamaged.
2. Press the tip cone carefully into a tip held in a tip tray.
If the volume is unlocked (the red bar is visible):
1. Turn the operating button to adjust the volume.
2. Slide the volume lock button down. Volume adjustment is now locked, preventing accidental volume changes.
NOTE: If the volume lock is not used, volume can be accidentally changed.
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