Sartorius Proline User Manual

User Manual
Bedienungsanleitung

Mode d'emploi

Manual Usuario

Instruzioni d'impiego

Инструкция пользователя

Proline

®

pipette

User Manual ............................................................1

Bedienungsanleitung .......................................... 15

Mode d’ emploi ..................................................... 29

Manual Usario ....................................................... 43

Instruzioni d’impiego ..........................................57

Инструкция пользователя

.................................73

Specifications ........................................................ 88

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på adressen www.sartorius.com

Proline

®

pipette

1

Contents

1. Intended use ..................................................................................................... 2

2. Product description ........................................................................................2

2.1. Sartorius Proline adjustable volume pipettes ....................... 2

2.2. Sartorius Proline fixed volume pipettes .................................. 3

2.3. Sartorius pipette tips ......................................................................3

3. Unpacking .......................................................................................................... 4

4. Installing the pipette holder ....................................................................... 4

5. Pipette Materials ............................................................................................. 5

6. Operating the pipette .................................................................................... 6

6.1. Volume setting .................................................................................. 6

6.2. Sealing and ejecting tips ............................................................... 6

6.3. Two-in-one pipettes ....................................................................... 6

6.4. Protective filters .............................................................................. 7

7. Pipetting techniques...................................................................................... 7

7.1. Forward pipetting ............................................................................ 7

7.2. Reverse pipetting ............................................................................. 8

8. Storage ................................................................................................................8

9. Testing the performance and recalibration ..........................................8

9.1. Testing the performance ............................................................... 9

9.2. Recalibration procedure ..............................................................11

10. Maintenance ................................................................................................... 11

10.1. Daily cleaning of the outer surface of the pipette ...........11

10.2. In-house maintenance .................................................................12

11. Troubleshooting ............................................................................................. 13

12. Warranty information ................................................................................. 14

2

1. Intended use

Proline pipette is intended to be used in liquid
handling applications for dispensing liquids
in total volume range from 0,1 µl to 5 ml. It is
recommended to use Optifit Tips or SafetySpace™
Filter tips to ensure the best compatibility and
performance with Sartorius pipettes.

This liquid handling device is designed and
manufactured to be used as general purpose
laboratory equipment. Before operating it is
recommended to read the user manual, which
contains useful information e.g. for good
pipetting practice.

2. Product description

Your new Proline hand-held pipette is a general
purpose pipette, for the accurate and precise
sampling and dispensing of liquid volumes. All
Proline pipettes operate on the basis of the air
displacement principle and use disposable tips.

The range of Sartorius Proline pipettes cover a
volume range from 0.1 µl to 5 ml.

2.1. Sartorius Proline adjustable
volume pipettes

Single channel pipettes:

Cat. No. Volume

Range/µl

Increment /µlTip/

µl

Safe-Cone Filters

50 pcs/bag

Standard Plus

720010 0.1-2.5 0.05 10 - -

720015 0.5-10 0.1 10, 200 - -

720080 2-20 0.5 200 721008 721018

720025 5-50 0.5 200, 350 721008 721018

720050 10-100 1 200, 350 721007 721017

720070 20-200 1 200, 350 721007 721017

720060 100-1000 5 1000 721006 721016

720110 1000-5000 50 5000 721005 721015

3

Multichannel pipettes:

2.2. Sartorius Proline fixed
volume pipettes

Single channel pipettes:

2.3. Sartorius pipette tips

Sartorius pipette tips are recommended for use
with Proline® Plus pipettes. The use of fully
compatible tips will ensure the maximal pipetting
accuracy and precision, and thereby guarantee
the performance specifications given for the
pipette. Sartorius tips are made of pure virgin
polypropylene and manufactured in protected
clean room conditions.

Sartorius offers a full range of standard Optifit,
and SafetySpace™ Filter tips. Sartorius non-filter
tips are available in trays, bulk packages and
space-saving refill systems. Sartorius non-filtered
tips and trays are autoclavable at 121°C (252F),

Cat. No. Volume Range/µlIncrement /µlTip/

µl

Safe-Cone Filters
50 pcs/bag
Standard

720210 8-ch 0.50-10 01 10 -

720220 8-ch 5-50 0.5 200, 350 721014

720240 8-ch 50-300 5 350 721014

720310 12-ch 5-10 0.1 10 -

720320 12- ch 5 -50 0.5 200, 350 721014

720340 12-ch 50-30 0 5 350 721014

Cat. No. Volume

Range/µl

Tip/
µl

Safe-Cone Filters

50 pcs/bag

Standard Plus

722001 5 10, 200 - -

722004 10 10, 200 - -

722010 20 200, 350 721008 721018

722015 25 200, 350 721008 721018

722020 50 200, 350 721008 721018

722025 100 200, 350 721007 721017

722030 200 200, 350 721007 721017

722035 250 1000 721006 721016

722040 500 1000 721006 721016

722045 1000 1000 721006 721016

722050 2000 5000 721005 721015

722055 5000 5000 721005 721015

4

20 min, 1 bar (15 p.s.i). All Single Tray Racks and
clean Refill Packs are certified RNase, DNase and
endotoxin-free.

For more information, please go to www.sartorius.
com or contact your local Sartorius support.

3. Unpacking

The pipette package contains the following items:

1. Pipette

2. Calibration/Opening tool

3. Grease

4. Instructions for use

5. Pipette holder

6. Tip

7. Filters (pipettes > 10 µl)

8. Performance certificate according to ISO
8655-6

4. Installing the pipette

holder

For convenience and safety always keep the
pipette vertically on its own holder when not in
use. When installing the holder, please follow the
instructions below:

1. Clean the shelf surface with ethanol.

2. Remove the protective paper from the
adhesive tape

3. Install the holder as described.

(Make sure the holder is pressed against the

edge of the shelf.)

4. Place the pipette onto the holder.

NOTE: An ergonomic, convenient carousel stand

for 6 Proline pipettes is also available (Cat.
No.725600).

5

5. Pipette Materials

Proline Single Channel

Push button Push button
rod (ABS) (Operating button)
(PA)
Grip Cover
(PA) Tip ejector
(PA)

Handle
(ASA/PC)
Display cover
(PC)

Tip Ejector
collar (PA)

Tip cone

2.5 –1000 µl (PVDF)
2000–5000 µl (PPS)

Push button Push button rod
(ABS) (PA)

Grip cover
(PA) Tip Ejector
(PA)

Handle
(ASA/PC)
Display cover
(PC)

Tip cone
housing
(ASA/PC)

Tip cone,
soft sealing
Tip ejector (PVDF)
bar (PETP) (TPU)
(PVDF)
Hard tip
cone
(PC)

Proline Multichannel

6

6. Operating the pipette

6.1. Volume setting

The volume of the pipette is clearly shown through
the handle display window. The delivery volume
(variable volume pipettes only) is set by turning
the operating button clockwise or anticlockwise.
When setting the volume, please make sure that:

1. The desired delivery volume clicks into place.

2. The digits are completely visible in the display
window.

3. The selected volume is within the pipette’s
specified range.

Do not turn the operating button outside the
volume range because it may jam the mechanism
and damage the pipette.

6.2. Sealing and ejecting tips

Sartorius tips are recommended for use with
Proline pipettes. Before fitting a tip ensure that
the pipette tip cone is clean. Press the tip on the
cone of the pipette firmly to ensure an airtight
seal. The seal is tight when a visible sealing ring
forms between the tip and the black tip cone.

Each pipette is fitted with a tip ejector to help
eliminate the safety hazards associated with
contamination. The tip ejector needs to be pressed
firmly downwards to ensure proper tip ejection.
Make sure that the tip is disposed of into a
suitable waste container.

6.3. Two-in-one pipettes

The Proline fixed volume pipettes 5 and 10 µl, and
variable volume pipettes 0.5-10 µl can be used
with both 10 µl and 200 µl tips. The pipettes are
supplied with two ejector collars and are factory
fitted with the ejector suitable for 10 µl tips.
When using 200 µl tips, please change the ejector
collar by following the instructions below:

1. Push the tip ejector down (1.).

2. Push the opening tool pin between the
ejector bar and ejector collar to release the
locking mechanism (2.).

3. Pull the ejector collar off (3.).

4. Install the ejector collar by pushing the collar
locking pin into the ejector bar hole, keeping
the tip ejector pushed down.

1.

2.

3.

7

5. Release the tip ejector and test that it runs
smoothly.

6.4. Protective filters

The new tip cones of Sartorius pipettes (>10
µl) allow the use of a exchangeable filter as an
option. The filter prevents liquids and aerosols
from entering the pipette. Two types of filters are
available: a Safe-Cone Filter Standard and a Safe­Cone Filter Plus (see cat.no. in Chapter 2.1). Plus
filters block the passage of liquids completely. The
filters do not affect the calibration of the pipette.

NOTE: Change the filter on the tip cone regularly

(after 50-250 pipettings).

7. Pipetting techniques

1. Make sure that the pipette, tips and the liquid
are at the same temperature.

2. Make sure that the tip is firmly attached to
the tip cone.

3. Hold the pipette vertically when aspirating
the liquid and place the tip only a few
millimetres into the liquid.

4. Prerinse the tip before aspirating the liquid
by filling and emptying the tip 5 times. This is
important especially when dispensing liquids
which have a viscosity and density different
from water.

5. Always control the operating button
movements with the thumb to ensure
consistency.

7.1. Forward pipetting

This technique employs the blow-out function
ensuring complete delivery of the liquid.

1. Depress the operating button to the first stop.

2. Place the tip(s) just under the surface of the
liquid (2-3 mm) and smoothly release the
operating button up to the starting position.
Carefully withdraw the tip from the liquid,
touching against the edge of the container
to remove excess.

3. Liquid is dispensed by gently depressing the
operating button to the first stop. After a
short delay continue to depress the operating
button to the second stop (= blow-out). This

Starting position

8

procedure will empty the tip(s) and ensure
accurate delivery.

4. Release the operating button to the starting
position. If necessary change the tip(s) and
continue pipetting.

7.2. Reverse pipetting

A selected volume plus an excess is aspirated
into the tip. The delivery is done without blow­out, and so the excess volume remains in the tip.
The reverse technique is recommended for high
viscosity, biological or foaming liquids, or very
small volumes of liquid.

1. Depress the operating button all the way to
the second stop. Place the tip(s) just under the
surface of the liquid (2-3 mm) and smoothly
release the operating button up to the
starting position.

2. Withdraw the tip(s) from the liquid touching
against the edge of the container to remove
excess.

3. Deliver the preset volume by smoothly
depressing the operating button to the first
stop. Hold the operating button at the first
stop. The liquid that remains in the tip(s)
should not be included in the delivery.

4. The remaining liquid should now be discarded
with the tip(s) or delivered back into the
container vessel.

NOTE: If reverse pipetting technique is used the

pipette might need recalibration.

8. Storage

When not in use it is recommended that your
Proline pipette is stored in a vertical position. See
Installing the pipette holder (Chapter 4).

9. Testing the

performance and
recalibration

It is recommended to check the performance of
your pipettes regularly (e.g. every 3 months) and
always after in-house maintenance. However,
the user should establish a regular testing
routine for their pipettes with regard to accuracy

First stop

Second stop

9

requirements of the application, frequency of use,
number of operators using the pipette, nature of
the liquid dispensed and the acceptable maximum
permissible errors established by the user. (ISO
8655-1.)

9.1. Testing the performance

Performance testing should take place in a
draught-free room at 15 – 30°C, constant to
+/- 0.5°C and humidity above 50%. The pipette,
tips and the test water should have stood in the
test room a sufficient time (at least 2 hours) to
reach equilibrium with the room conditions. Use
distilled or deionised water (grade 3, ISO 3696).
Use an analytical balance with a readability of

0.01 mgs. (ISO 8655-6.)

Weighing

1. Adjust the desired test volume Vs.

2. Carefully fit the tip onto the tip cone.

3. Fill the tip with test water and expel to waste
five times to reach a humidity equilibrium in
the dead air volume.

4. Replace the tip. Pre-wet the tip by filling it
once with test water and expel to waste.

5. Aspirate the test water, immersing the tip
only 2-3 mm below the surface of the water.
Keep the pipette vertical.

6. Withdraw the pipette vertically and touch the
tip against the inside wall of the test water
container.

7. Pipette the water into the weighing vessel,
touching the tip against the inside wall of
the vessel just above the liquid surface at an
angle of 30° to 45°. Withdraw the pipette by
drawing the tip 8-10 mm along the inner wall
of the weighing vessel.

8. Read the weight in mgs (mi).

9. Repeat the test cycle until 10 measurements
have been recorded.

10. Convert the recorded masses (mi) to volumes
(Vi)

Vi = mi Z Z = correction factor (Table 1)

11. Calculate the mean volume (⊽) delivered:

⊽ = (∑Vi)/10

12. For conformity evaluation calculate the
systematic error es of the measurement:

in µl: es= ⊽ - Vs Vs = selected test volume

or in %: es = 100 (⊽ - Vs)/V

s

13. For conformity evaluation calculate the
random error of the measurement:

10

as standard deviation s = ∑(Vi - ⊽)2

n - 1

n = number of measurement (10)

or as coefficient of variation CV = 100s/⊽

14. Compare the systematic error (inaccuracy)
and random error (imprecision) with the
values in the performance specifications

- (p. 88) or the specifications of your own
laboratory.

If the results fall within the specifications,

the pipette is ready for use. Otherwise check
both systematic and random errors and,
when necessary, proceed to the recalibration
procedure (Chapter 9.2).

NOTE: Systemic error (inaccuracy) is the

difference between the dispensed volume and the
selected test volume. Random error (imprecision)
is the scatter of the dispensed volumes around
the mean of the dispensed volume. (ISO 8655-1.)

NOTE: Sartorius specifications are achieved in

strictly controlled conditions (ISO 8655-6). The
user should establish own specifications based on
the field of use and the accuracy requirements
placed on the pipette (ISO8655-1).

Table 1

Z-values (µl/mg):

Temp. (°C) Air Pressure (kPa)

95 100 101.3 105

20.0

20.5

21.0

21.5

22.0

22.5

23.0

23.5

1.0028

1.0029

1.0030

1.0031

1.0032

1.0033

1.0034

1.0036

1.0028

1.0029

1.0031

1.0032

1.0033

1.0034

1.0035

1.0036

1.0029

1.0030

1.0031

1.0032

1.0033

1.0034

1.0035

1.0036

1.0029

1.0030

1.0031

1.0032

1.0033

1.0034

1.0036

1.0037

NOTE: This method is based on ISO 8655.

11

9.2. Recalibration procedure

1. Place the calibration tool into the holes of
the calibration adjustment lock (under the
operating button).

2. Turn the adjustment lock anticlockwise
to decrease and clockwise to increase the
volume.

3. Repeat Performance test (Chapter 9.1.)
procedure from step 1 until the pipetting
results are correct.

10. Maintenance

To maintain the best results from your pipette
each unit should be checked every day for
cleanliness. Particular attention should be paid to
the tip cone(s).

Proline pipettes have been designed for easy in­house service. However, Sartorius also provides
complete repair and recalibration service
including a service report and performance
certificate(s). Please return your pipette to your
local representative for repair or performance
test/ recalibration. Before returning please
make sure that it is free from all contamination.
Please advise our Service Representative of any
hazardous materials which may have been used
with your pipette.

NOTE: Check the performance of your pipette

regularly e.g. every 3 months and always after in­house service or maintenance.

10.1. Daily cleaning of the outer

surface of the pipette

Your Proline pipette should be checked every day
for cleanliness. To clean and decontaminate the
outer surface of your pipette use ethanol (70%),
isopropanol (60%) or a mild detergent and a soft
lint-free cloth.

Gently clean the surface of the pipette with
moistened cloth and wipe dry. Pay special
attention to the tip cone. Change the tip cone
filter if needed.

+

–

12

10.2. In-house maintenance

1. Hold down the tip ejector.

2. Place the tooth of the opening tool between
the tip ejector and the tip ejector collar to
release the locking mechanism.

3. Carefully release the tip ejector and remove
the tip ejector collar.

4. Place the wrench end of the opening tool
over the tip cone, turning it anticlockwise. Do
not use any other tools. The 5 ml tip cone is
removed by turning it anticlockwise without
any tools.

5. Remove the tip cone, piston, and spring.
Remove the filter if fitted.

6. Clean the tip ejector collar, the tip cone
holder, the tip cone (cylinder) and the piston
with ethanol (70%), isopropanol (60%) or mild
detergent and soft lint-free cloth.

7. Clean the interior of the tip ejector collar and
the tip cone (cylinder) with a cotton swab.

8. Before replacing the tip cone it is
recommended to grease the piston and
O-ring slightly by using the silicone grease
provided.

9. After reassembling press the operating
button several times to ensure that the grease
has spread evenly.

10. Check the pipette calibration.

NOTE: On models 720010, 720015, 722001 and

722004 (10 µl pipettes) the O-ring cannot be
accessed for maintenance.

NOTE: Excessive use of grease may jam the piston.

1.

2.

3.

2.

1.

2.

13

11. Troubleshooting

Symptom Possible cause Solution

Droplets left
inside the tip

Unsuitable tip Use original

Sartorius tips

Non-uniform wetting
of the tip plastic

Attach new tip

Leakage or pipetted
volume too small

Tip incorrectly
attached

Attach firmly

Unsuitable tip Use original

Sartorius tips

Foreign particles
between tip and cone

Clean the tip cone,
attach new tip

Instrument
contaminated

Clean and grease
O-ring and piston,
clean the tip cone

Insufficient amount of
grease on piston and
O-ring

Grease accordingly

O-ring not correctly
positioned or damaged

Change the O-ring

Incorrect operation Follow instructions

carefully

Calibration altered
or unsuitable for the
liquid

Recalibrate
according to
instructions

Instrument damaged Send for service

Push button
jammed or moves
erratically

Piston contaminated Clean and grease

O-ring and piston,
clean the tip cone

Penetration of solvent,
vapours

Clean and grease
O-ring and piston,
clean the tip cone

Pipette blocked,
aspirated volume
too small

Liquid has penetrated
tip cone and dried

Clean and grease
O-ring and piston,
clean the tip cone

Tip ejector jammed
or moves erratically

Tip cone and/or ejector
collar contaminated

Clean the tip cone
and the ejector
collar

14

12. Warranty information

The Proline pipettes are covered by warranty
for 2 years against defects in materials and
workmanship. Should your pipette fail to function
at any time, please contact your local Sartorius
representative.

ANY WARRANTY WILL, HOWEVER, BE DEEMED AS
VOID IF FAULT IS FOUND TO HAVE BEEN CAUSED
BY MALTREATMENT, MISUSE, UNAUTHORIZED
MAINTENANCE OF SERVICE OR NEGLIGENCE
OF REGULAR MAINTENANCE AND SERVICE,
ACCIDENTAL DAMAGE, INCORRECT STORAGE
OR USE OF THE PRODUCTS FOR OPERATIONS
OUTSIDE THEIR SPECIFIED LIMITATIONS, OUTSIDE
THEIR SPECIFICATIONS, CONTRARY TO THE
INSTRUCTIONS GIVEN IN THIS MANUAL OR WITH
OTHER THAN THE MANUFACTURER’S ORIGINAL
TIPS.

Each pipette is tested before shipping by the
manufacturer. The Sartorius Quality Assurance
Procedure guarantees that the Sartorius Proline
pipette you have purchased is ready for use.

Specifications

Proline Single Channel Adjustable Volume Pipette

Cat.No. Volume

range/µl

Volume
µl

Inaccuracy% Imprecision

%

720005 0.1-2.5 2.50

1.25

0.25

2.50

3.00

12. 00

2.00

3.00

6.00

720000 0.5-10 10

5
1

1.00

1.50

2.50

0.80

1.50

1.50

720080 2-20 20

10
2

0.90

1.20

3.00

0.40

1.00

2.00

720020 5-50 50

25
5

0.60

0.90

2.00

0.30

0.60

2.00

720050 10-100 100

50
10

0.80

1.00

3.00

0.20

0.40

1.00

720070 20-200 200

100
20

0.60

0.80

2.50

0.20

0.30

0.80

720060 100-1000 1000

500
100

0.60

0.70

2.00

0.20

0.25

0.70

720110 1-5 5

2.5
1

0.50

0.60

0.70

0.20

0.30

0.30

Proline Multichannel Adjustable Volume Pipettes

Cat.No. Volume

range/µl

Volume
µl

Inaccuracy
µl

Imprecision
µl

720210 8-ch

0.5-10

10
5
1

1.50

2.50

4.00

1.50

2.50

4.00

720220 8-ch

5-50

50
25
5

1.00

1.50

3.00

0.50

1.00

2.00

720240 8-ch

50-300

300
150
50

0.70

1.00

1.50

0.25

0.50

0.80

720310 12-ch

0.5-10

10
5
1

1.50

2.50

4.00

1.50

2.50

4.00

720320 12-ch

5-50

50
25
5

1.00

1.50

3.00

0.50

1.00

2.00

720340 12-ch

50-300

300
150
50

0.70

1.00

1.50

0.25

0.50

0.80

Proline Fixed Volume Pipettes

Cat.No. Volume

range/µl

Volume
µl

Inaccuracy
µl

Imprecision
µl

722001 5 5 1.30 1.20

722004 10 10 0.80 0.80

722010 20 20 0.60 0.50

722015 25 25 0.50 0.30

722020 50 50 0.50 0.30

722025 100 100 0.50 0.30

722030 200 200 0.40 0.20

722035 250 250 0.40 0.20

722040 500 500 0.30 0.20

722045 1000 1000 0.30 0.20

722050 2000 2000 0.30 0.15

722055 5000 5000 0.30 0.15

Liquid: Distilled water (grade 3, ISO 3696)
Reference temperature: 22°C. constant to ± 0.5°C
Tested: According to ISO 8655 using original

Sartorius Optifit Tips

4002040 • 03/2013 • © Sartorius Biohit Liquid Handling Oy

Sartorius Biohit Liquid Handling Oy

Laippatie 1

FI-00880 Helsinki
Finland

Phone +358.755.951
[e-mail]
www.sartorius.com

Headquarter

Sartorius Corporate

Administration GmbH
Weender Landstrasse 94-108
37075 Goettingen, Germany

Phone [phone]
Fax [phone]
www.sartorius.com

Copyright by Sartorius, Helsinki,
Finland.

All rights reserved. No part of this
publication may be reprinted or
translated in any form or by any
means without the prior written
permission of Sartorius.

The status of the information,
specifications and illustrations in this
manual is indicated by the date given
aside.

Sartorius reserves the right to make
changes to the technology, features,
specifications and design of the
equipment without notice.

All trademarks are Sartorius property
unless otherwise stated. Patents
granted or pending.