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PICUS,PICUS NXT
User Manual
44 pgs3.94 Mb0

Sartorius PICUS,PICUS NXT User Manual

User Manual
Picus® & Picus® NxT Electronic Pipette
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Table of Contents
1. Introduction 3
1.1. Intended Use 4
1.2. Product Overview 4
1.3. Contents of Delivery Package 6
2. Getting Started 7
2.1. Charging 7
2.2. Power Up 7
3. Operation 8
3.1. Operating Principles 8
3.2. Pipetting Modes 9
3.3. Saving Pipetting Programs to Memory 20
3.4. Setup 21
4. Care and Maintenance 30
4.1. Cleaning and Maintenance 30
4.2. Sterilizing 33
4.3. Performance Testing 33
4.4. Replacing the Battery 35
4.5. Replacement Parts 35
4.6. Storage 35
5. Warranty 35
6. Hazardous Substances (RoHS II) 36
7. Disposal (WEEE) 36
8. Troubleshooting 36
8.1. Hardware Reset 36
8.2. Troubleshooting Guide 36
9. Technical Data 37
9.1. Performance Specifications 38
9.2. Speed Table 38
10. Ordering Information 40
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1. Introduction
The Sartorius Picus®/Picus® NxT electronic pipette brings ergonomics to a completely new level with its exceptionally lightweight and compact design, and its ease of operation. This versatile air displacement pipette has been designed by an experienced R&D team in cooperation with laboratory personnel and ergonomists to ensure safe and comfortable pipetting.
The Picus®/Picus® NxT is available in single and multichannel models (8 and 12-channel). Tips can be attached and ejected safely and comfortably using the Optiload tip-loading feature and the electronic tip ejection function. To improve safety by reducing the risk of contamination, replaceable Safe-Cone Filters can be used in all Picus®/Picus® NxT models greater than 10 µl.
The fully electronic operation, unique DC motor, and the electronic brake and piston control system guarantee excellent accuracy and precision. The desired volume can be quickly selected using the light and easy-to-use adjustment wheel on top of the pipette.
Picus® NxT offers advanced safety and efficiency features for highly regulated laboratories. These features include a certificate of accredited 3-point calibration, user-definable pipetting protocols, and calibration and service reminders.
Congratulations on becoming a new Picus®/Picus® NxT owner!
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1.1. Intended Use
The Picus®/Picus® NxT pipette is intended, designed and manufactured for dispensing liquids in a variety of applications and to be used in combination with Optifit Tips or SafetySpace Filter Tips. The aforementioned pipette and tip combination, fall within the scope of in-vitro diagnostics, and can be used as a diagnostic medical device in related applications. Thereby, Picus®/Picus® NxT and its accessories fulfil the relevant demands of the Directive 98/97/EC of the European Parliament.
The Picus®/Picus® NxT product range covers a volume range of 0,2 µl to 10 ml. It is recommended that Sartorius Optifit Tips or SafetySpace Filter Tips are used with Sartorius pipettes to ensure optimum compatibility and performance.
It is a general purpose laboratory device that fulfils ISO 9001 and ISO 13485 standards.
This manual contains useful information, including information on good pipetting practice.
NOTE: Prolonged pipetting can cause Work Related Upper Limb Disorder
(WRULD). The manufacturer is not responsible for WRULD or any related injuries caused by using pipettes.
1.2. Product Overview
Before using the Sartorius Picus®/Picus® NxT electronic pipette for the first time, please review this operating manual carefully. This manual can be downloaded from the Sartorius website at: www.sartorius.com. Hardcopies may be ordered by e-mail from lhinfo.finland@sartorius.com.
1.2.1. Single and Multichannel Pipettes
1. Electronic tip ejector
2. Charging contacts
3. ON/OFF button
4. USB-charging port
5. Operating button & volume range colour-code
6. Adjustment wheel
7. Hotkey for memory places (black in Picus® NxT)
8. Softkeys for programming (black in Picus® NxT)
9. Dot-matrix display
10. Maximum volume
11. Dispensing head (tip ejector collar and tip-cone), autoclavable (excl. 8/12-ch 1200 µl)
12. Optiload, spring-loaded tip-cones in multichannel pipettes
13. Safe-Cone Filters (excl. <10 µl pipettes)
14. Pipette tip
15. Tip ejector collar. (When using non-Sartorius tips, it can be adjusted for suitable tip ejection, in 10µl, 120µl, 300µl and 1000µl single-channel models)
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SafetySpace air gap
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1.2.2 Display
The multi-colour backlit dot-matrix display is clear and is logically structured.
1. Softkey functions
2. Current mode
3. Pipetting volume and aliquots in multidispensing modes
4. Speed
5. Battery charging indicator
6. Arrows indicating aspiration and dispensing
1.2.3 Optifit Tips and SafetySpace Filter Tips
We recommend using Sartorius Optifit Tips or SafetySpace Filter Tips. These tips are designed for Sartorius pipettes and therefore ensure optimal compatibility, accuracy, and precision.
For optimum results you should also:
−Ensure that the liquid and the pipette/tip combination are at approximately the same temperature
−Choose the right tip volume for your pipette: the colour of the tip tray should match the colour code on the pipette
−Use Optifit Tips in rack, refill, or bulk packaging, and choose the purity level your application requires: free of DNase, RNase and endotoxin, and/or sterilized
−If aerosol contamination needs to be avoided, choose Safe-Cone Filters, which are attached to the tip-cone and should be changed daily, or SafetySpace Filter Tips, which are discarded after each pipetting
−SafetySpace Filter Tips should also be chosen when you want to avoid sample loss due to the sample reaching the filter. The extra space between the sample and the filter ensures that even foaming or viscous liquids don’t reach the filter in reverse pipetting or repetitive/multiple dispensing modes
−Pre-rinse the tip three to five times before pipetting (this is especially important in the forward pipetting mode)
−Wipe the tip against the receiving vessel’s wall to catch the last droplet after dispensing
−Change the tip after every pipetting
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1.3. Contents of Delivery Package
−Picus®/Picus® NxT electronic pipette
− Universal USB charger (EU, UK, US, JPN, KOR, AUS, and CHN plugs)
− Sartorius tip rack (10x96 tips) of corresponding volume with up to 1000 µl single-channel models / 1200 µl multichannel models
− One tip with 5 ml and 10 ml single-channel models
− Safe-Cone Filters and tweezers with >10 µl models
− Autoclavable grease with the single-channel models
− QC-certificate (Picus®), certificate of accredited 3-point calibration (Picus® NxT)
− Quick Start Guide
If any item in this delivery package is missing or damaged, please contact your local Sartorius representative.
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2. Getting Started
Please read this manual before using your Picus®/Picus® NxT electronic pipette.
1. Your Picus®/Picus® NxT is delivered, with the battery partially charged. We recommend the pipette is charged fully, before first use; charge at least one (1) hour.
2. Insert a replaceable Safe-Cone Filter into the tip-cone before use, to prevent contamination of the pipette.
3. Press the ON/OFF button on the top of the pipette to turn the power on.
4. Press the tip ejector button when advised by the display.
5. The pipette is now ready to be programmed and used.
2.1. Charging
It is recommended that you charge the pipette before first use for one (1) hour.
−Charging via USB: Connect the USB cable to the pipette and plug the charger into the mains power outlet.
−Charging using charging stand or carousel: Make sure that the charging stand is connected to the mains power outlet via the AC charger, and that the charging contacts of the pipette are properly in contact with the charging grooves of the charging stand.
Charging options for Sartorius electronic pipettes:
−USB charger, universal, included in the package
− Sartorius Charging Stand for one pipette, order code: 730981
− Sartorius Charging Carousel for four pipettes, order code: 730991
The battery sign in the bottom right corner of the display indicates the battery’s level of charge. When the battery is low, the indicator blinks LOW and the pipette needs to be charged.
NOTE! Before connecting the AC charger to the charging stand and the mains
electrical outlet, make sure that the power supply output voltage level and power capacity are correct. The use of incorrect power supplies may damage the device. Only use the power supplies recommended by the manufacturer.
2.2. Power Up
1. Press ON/OFF button: pipette will turn on.
2. Press the tip ejector button as advised on the display. The pipette is now ready to be programmed and used.
3. While being used and/or charged, the pipette is in active mode, all the processor functions are activated and the display backlight is on.
4. One minute after last activity the pipette will switch to power saving mode and the backlight will dim. The pipette returns to active mode when any buttons are pressed or if the adjustment wheel is turned.
5. 10 minutes after last activity the backlight turns off, but the display is still visible. The pipette returns to active mode when any buttons are pressed or if the adjustment wheel is turned.
6. 60 minutes after last activity the pipette turns off. To power up the pipette again, press either operating button or the ON/OFF button. The pipette will also turn on when charged.
If the pipette is turned off from the ON/OFF button, it can only be turned on by again using the same button.
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3. Operation
3.1. Operating Principles
Pipetting functions are controlled using the operating button, adjustment wheel, and Softkeys. The Hotkey is used to store or activate programs.
Operating button
−Confirms settings (can be used as an alternative to the Softkey for OK) and initiates piston movement for aspiration, dispensing and repeated blow-out.
Adjustment wheel
−Scrolls the menu and adjusts volume settings.
−Moves the piston in manual and titrating modes for aspiration and dispensing.
−Unlocks volume adjustment for editing when turned fully in one direction.
Electronic tip ejector
−Ejects the tip(s) with the light touch of a finger
Left Softkey
− MENU: Displays the main mode selection.
−BACK: Exits the currently displayed, MENU, EDIT, or ADV functions, without saving changes.
−QUIT: Quits the pipetting task.
−LOCK: Shown when reminders are triggered. Locks the pipette.
Middle Softkey
−EDIT: Activates the editing mode so that settings can be changed.
−NEXT: Active in editing mode. Confirms a setting and moves to the next one.
−SAVE: Active in memory settings. Saves the current program to the selected memory place.
− ABC/abc/123/#@!/CLEAR: Active in memory settings and setup settings. Allows you to select letters, numbers, or symbols, or clear existing text.
−RESET: Active in the reminders menu. Resets the date and cycle counters.
−PREV: Active in editing mode. Moves back to the previous setting.
−SNOOZE: Shown when reminders are triggered. Snoozes the reminder.
−UNLOCK: Active when pipette is locked. Unlocks the pipette. If password protection is enabled, the administrator password is required.
Right Softkey
−ADV: Displays available advanced functions for the activated main mode.
−OK: Confirms the setting or selection and exits.
Hotkey
−10 memory places to save and activate frequently used or favorite pipette settings.
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3.2. Pipetting Modes
The Picus® NxT electronic pipette has nine (9) main pipetting modes, while the Picus® has eight (8). The Picus® NxT has seven (7) advanced functions and the Picus® has six (6).
Pipetting Modes Advanced Functions (ADV)*
Tracker Mixing Counter Excess Volume
Adjustment
Auto Dispensing
Repeated Blow-out**
Fast Dispensing
Pipetting
Reverse Pipetting
Multi-Dispensing
Manual Pipetting
Diluting
Sequential Dispensing
Multi-Aspiration
Titration
Protocols **
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* Are used in conjunction with pipetting modes ** Only in Picus® NxT models. *** Availabilty of the advanced functions varies according to the pipetting mode selected for the protocol.
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Press the Softkey for MENU
Press the Softkey for EDIT
3.2.1. Pipetting (Forward Pipetting)
Pipetting mode aspirates and then dispenses the selected volume of liquid. It is recommended for aqueous liquids, liquids containing small amounts of detergent or proteins, and solvents.
To select Pipetting mode:
1. Select MENU by pressing the left Softkey.
2. Confirm Pipetting mode by pressing the operating button or the right Softkey for OK. The latest settings used will then be displayed.
To edit the volume and speed settings:
1. Press the middle Softkey for EDIT or turn the adjustment wheel all the way to the right or left. The first setting to be edited is now highlighted.
2. Turn the adjustment wheel to set the desired value.
3. Confirm the setting by pressing
a. the operating button or the right Softkey for OK, which will exit the
editing mode.
OR
b. the middle Softkey for NEXT, to move on to edit the next highlighted
setting.
4. Repeat steps 2 and 3 for all the settings you wish to edit.
5. To exit the editing mode without saving changes, press the left Softkey for BACK.
To use the pipette with the selected program:
1. Press the operating button to aspirate the liquid.
2. Press the operating button again to dispense the liquid.
3. Eject the tip by pressing the electronic tip ejector button.
Advanced Functions
Counter, Mixing, and Tracker can be used in conjunction with Pipetting mode. In Picus® NxT, Repeated Blow-out can also be used. Repeated Blow-out helps to remove residual liquid from the tip, for liquids where normal dispensing does not empty it sufficiently.
−Counter counts the number of times a liquid is dispensed. Counting can be set to start at any number.
−Mixing mixes liquids manually or automatically. The mixing volume can be adjusted according to the pipette’s maximum volume.
−Tracker displays the location to next dispense liquid in a microplate.
For more information on using advanced functions see section 3.2.10.
NOTE! Only one advanced function can be selected at a time. Repeated Blow-
out can be combined with other advanced functions.
3.2.2. Reverse Pipetting
Reverse Pipetting aspirates the selected volume as well as an excess volume. It is recommended for biological, foaming, and viscous liquids. In Reverse Pipetting mode the excess is left inside the tip and then discarded.
To select Reverse Pipetting mode:
1. Select MENU by pressing the left Softkey.
2. Use the adjustment wheel to select Reverse and confirm by pressing the operating button or the right Softkey for OK. The latest settings used will then be displayed.
To edit the volume and speed settings:
1. Press the middle Softkey for EDIT or turn the adjustment wheel all the way to the right or left. The first setting to be edited is now highlighted.
Change settings and press OK or NEXT
Choose the mode
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2. Turn the adjustment wheel to set the desired value.
3. Confirm the setting by pressing
a. the operating button or the right Softkey for OK, which will exit the
editing mode.
OR
b. the middle Softkey for NEXT, to move on to edit the next highlighted
setting.
4. Repeat steps 2 and 3 for all settings you wish to edit.
5. To exit the editing mode without saving changes, press the left Softkey for BACK.
To use the pipette with the selected program:
1. Press the operating button to aspirate the liquid (selected volume + excess).
2. Press the operating button again to dispense the selected volume.
3. To continue reverse pipetting without discarding the excess volume, press the left Softkey for NO and return to step 1.
4. To discard the excess volume and empty the tip, press the operating button twice.
5. Eject the tip by pressing the electronic tip ejector button.
Advanced Functions
Counter, Excess Adjustment, and Tracker can be used in conjunction with Reverse Pipetting.
−Counter counts the number of times a liquid is dispensed. Counting can be set to start at any number.
−Excess Adjustment can be used to set the excess volume. Otherwise a default value is used.
−Tracker displays the location to next dispense liquid in a microplate.
For more information on using advanced functions see section 3.2.10.
NOTE! Only one advanced function can be selected at a time.
3.2.3. Multi-Dispensing
Multi-Dispensing aspirates the total volume as well as an excess volume, then repetitively dispenses equal volumes of liquid. It is recommended for long pipetting series and microplate dispensing.
To select Multi-Dispensing mode:
1. Select MENU by pressing the left Softkey.
2. Use the adjustment wheel to select Multi-Disp and confirm by pressing the operating button or the right Softkey for OK. The latest settings used will then be displayed.
To edit the settings for volume, speed, and number of dispensings:
1. Press the middle Softkey for EDIT or turn the adjustment wheel all the way to the right or left. The first setting to be edited is now highlighted.
2. Turn the adjustment wheel to set the desired value.
3. Confirm the setting by pressing
a. the operating button or the right Softkey for OK, which will exit the
editing mode.
OR
b. the middle Softkey for NEXT, to the next highlighted setting.
4. Repeat steps 2 and 3 for all settings you wish to edit.
5. To exit the editing mode without saving changes, press the left Softkey for BACK.
To use the pipette with the selected program:
1. Press the operating button to aspirate the liquid (selected volume + excess).
2. Press the operating button again to discard the prime excess.
Dispensing 10 µl twelve times.
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3. Press the operating button repeatedly until all aliquots have been dispensed.
4. To continue repetitive dispensing without discarding the excess volume, press the left Softkey for NO and return to step 1.
5. To discard the excess volume and to empty the tip, press the operating button twice.
6. Eject the tip by pressing the electronic tip ejector button.
Advanced Functions
Excess Adjustment, timed Automated Dispensing, and Tracker can be used in conjunction with Multi-Dispensing.
−Excess Adjustment can be used to set the excess volume.
−Timed Automated Dispensing dispenses automatically without needing the operating button to be pushed each time.
−Tracker displays the location to next dispense liquid in microplate dispensing.
For more information on using advanced functions see section 3.2.10.
NOTE! Only one advanced function can be selected at a time.
3.2.4. Manual Pipetting
In Manual Pipetting the piston movement in aspiration and dispensing is controlled manually by turning the adjustment wheel. It is ideal for measuring reagents, and for applications in which the pipetting speed needs to be controlled manually.
To select Manual Pipetting mode:
1. Select MENU by pressing the left Softkey.
2. Use the adjustment wheel to select Manual and confirm by pressing the operating button or the right Softkey for OK.
To edit the volume and speed settings:
1. Press the middle Softkey for EDIT or turn the adjustment wheel all the way to the right or left. The first setting to be edited is now highlighted.
2. Turn the adjustment wheel to set the desired value.
3. Confirm the setting by pressing
a. the operating button or the right Softkey for OK, which will exit the
editing mode.
OR
b. the middle Softkey for NEXT, to move on to edit the next highlighted
setting.
4. Repeat steps 2 and 3 for all settings you wish to edit.
5. To exit the editing mode without saving changes, press the left Softkey for BACK.
To use the pipette with the selected program:
1. Press the operating button to begin aspiration.
2. To aspirate the liquid, press the operating button again and hold it down, or turn the adjustment wheel to the right. The aspiration speed can be adjusted by how far the adjustment wheel is turned. To pause aspiration, briefly release the operating button or adjustment wheel.
3. To begin dispensing, turn the adjustment wheel to the left. Hold the adjustment wheel to the left or press the operating button down to continue dispensing.
4. When the entire volume is dispensed, press the operating button to empty the tip or press the left Softkey for NO to continue with aspiration.
5. Eject the tip by pressing the electronic tip ejector button.
Manual pipetting
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Advanced Functions
Repeated Blow-out, available with Picus® NxT, can be used in conjunction with Manual Pipetting mode for liquids where normal dispensing does not completely empty the tip.
For more information on using advanced functions see section 3.2.10.
3.2.5. Diluting
In Dilution mode liquids separated by an air gap are aspirated and then dispensed simultaneously. Diluting can be used to dilute samples and reagents. The diluent is aspirated first followed by an air gap, then the sample or reagent to avoid contamination.
To select Diluting mode:
1. Select MENU by pressing the left Softkey.
2. Use the adjustment wheel to select Diluting and confirm by pressing the operating button or the right Softkey for OK.
To edit the volume and speed settings:
1. Press the middle Softkey for EDIT or turn the adjustment wheel all the way to the right or left. The first setting to be edited is now highlighted.
2. Turn the adjustment wheel to set the desired value.
3. Confirm the setting by pressing
a. the operating button or the right Softkey for OK, which will exit the
editing mode.
OR
b. the middle Softkey for NEXT, to move on to edit the next highlighted
setting.
4. Repeat steps 2 and 3 for all settings you wish to edit.
5. To exit the editing mode without saving changes, press the left Softkey for BACK.
To use the pipette with the selected program:
1. Press the operating button to aspirate the diluent.
2. Press the operating button again to aspirate the air gap.
3. Press the operating button a third time to aspirate the sample.
4. Dispense the entire volume by pressing the operating button.
5. Empty the tip by pressing the operating button again.
6. Eject the tip by pressing the electronic tip ejector button.
Advanced Functions
Repeated Blow-out (Picus® NxT only) and Mixing can be used in conjunction with Diluting.
−Mixing mixes liquids manually or automatically. The mixing volume can be adjusted according to the pipette’s maximum volume.
−Repeated Blow-out helps to remove residual liquid from the tip, for liquids where normal dispensing does not empty it sufficiently.
For more information on using advanced functions see section 3.2.10.
3.2.6. Sequential Dispensing
Sequential Dispensing repeatedly dispenses selected volumes in any desired order. This is a useful mode for diluting series and making calibration curves.
To select Sequential Dispensing mode:
1. Select MENU by pressing the left Softkey.
2. Use the adjustment wheel to select Seq. Disp. and confirm by pressing the operating button or the right Softkey for OK. The latest settings used will then be displayed.
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To edit the settings for speed, number of aliquots, and aliquot volumes:
1. Press the middle Softkey for EDIT or turn the adjustment wheel all the way to the right or left. The first setting to be edited is now highlighted.
2. Turn the adjustment wheel to set the desired value.
3. Confirm the setting by pressing
a. the operating button or the right Softkey for OK, which will exit the
editing mode.
OR
b. the middle Softkey for NEXT, to move on to edit the next highlighted
setting.
4. Repeat steps 2 and 3 for all settings you wish to edit.
5. To exit the editing mode without saving changes, press the left Softkey for BACK.
To use the pipette with the selected program:
1. Press the operating button to aspirate the selected volume.
2. Press the operating button again for pre-out to make sure that the first aliquot will be of the correct volume.
3. Dispense the set aliquots by pressing the operating button each time.
4. After the last aliquot has been dispensed, press the operating button to empty the tip or press NO to start aspirating without emptying the remaining liquid.
5. Eject the tip by pressing the electronic tip ejector button.
Advanced Functions
Repeated Blow-out (Picus® NxT only) and Excess Adjustment can be used in conjunction with Sequential Dispensing.
−Excess Adjustment can be used to set the excess volume.
−Repeated Blow-out helps to remove residual liquid from the tip, for liquids where normal dispensing does not empty it sufficiently.
For more information on using advanced functions see section 3.2.10.
3.2.7. Multi-Aspirating
Multi-Aspiration aspirates a selected volume a set number of times. Multi­Aspirating is useful for sample pooling and microplate washing. Select the volume and the number of aspirations, aspirate until the series is completed, then discard the full aspirated volume in a single step.
To select Multi-Aspiration mode:
1. Select MENU by pressing the left Softkey.
2. Use the adjustment wheel to select Multi-Aspiration and confirm by pressing the operating button or the right Softkey for OK. The latest settings used will then be displayed.
To edit the settings for speed, volume, and number of aspirations:
1. Press the middle Softkey for EDIT or turn the adjustment wheel all the way to the right or left. The first setting to be edited is now highlighted.
2. Turn the adjustment wheel to set the desired value.
3. Confirm the setting by pressing
a. the operating button or the right Softkey for OK, which will exit the
editing mode.
OR
b. the middle Softkey for NEXT, to move on to edit the next highlighted
setting.
4. Repeat steps 2 and 3 for all settings you wish to edit.
5. To exit the editing mode without saving changes, press the left Softkey for BACK.
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