Sartorius mLINE User Manual
User Manual
Bedienungsanleitung
Mode d'emploi
Manual Usuario
Instruzioni d'impiego
Инструкция пользователя
mLINE
®
pipette
User Manual ............................................................1
Bedienungsanleitung .......................................... 17
Mode d’ emploi ..................................................... 35
Manual Usario ....................................................... 53
Instruzioni d’impiego ..........................................71
Инструкция пользователя
.................................89
Specifications ......................................................109
Huom! Suomenkielinen käyttöohje ladattavissa
osoitteesta: www.sartorius.com
Obs: Manual på svenska kan du ladda ner
på adressen www.sartorius.com
mLINE
®
pipette
1
Contents
1. Intended use ..................................................................................................... 2
2. Product description ........................................................................................2
3. Sartorius pipette tips .....................................................................................4
4. Unpacking .......................................................................................................... 4
5. Personal identification .................................................................................. 5
6. Pipette holder and carousel stand ............................................................5
6.1. Installing the pipette holder ........................................................ 5
6.2. Carousel stand ..................................................................................5
7. Calibration tool (also tube and ID window opener) ........................... 5
8. Operating the pipette .................................................................................... 6
8.1. Setting the volume ..........................................................................6
8.2. Sealing and ejecting tips ............................................................... 6
8.3. Protective tip cone filters ............................................................. 6
9. Pipetting techniques...................................................................................... 7
9.1. Forward pipetting ............................................................................ 7
9.2. Reverse pipetting ............................................................................. 7
9.3. Repetitive reverse technique ....................................................... 8
10. Recommendations for good pipetting ....................................................8
11. Maintenance ..................................................................................................... 9
11.1. Daily cleaning of the outer surface of the pipette ............. 9
11.2. Cleaning the lower part of the pipette ....................................9
11.3. Sterilization of the pipette ......................................................... 11
12. Testing the performance and recalibration ........................................11
12.1. Testing the performance ............................................................. 12
12.2. Recalibration....................................................................................13
13. Troubleshooting ............................................................................................. 15
14. Warranty information .................................................................................16
15. Performance specifications ......................................................................16
2
1. Intended use
mLINE pipette is intended to be used in liquid
handling applications for dispensing liquids in
volume range of 0,1 µl to 10 ml. It is recommended
to use Optifit Tips or SafetySpace™ Filter Tips to
ensure the best compatibility and performance
with Sartorius pipettes.
This liquid handling device is designed and
manufactured to be used as general purpose
laboratory equipment. Before operating it is
recommended to read the user manual, which
contains useful information e.g. for good
pipetting practice.
2. Product description
Your new mLINE is an autoclavable air
displacement pipette. The attractive and
ergonomical design of the mLINE pipette together
with its very low pipetting forces reduce the risk
of repetitive strain injuries (RSI). The pipette is
designed for both right- and left-handed use.
NOTE: It is known that prolonged pipetting can
cause RSI. The manufacturer is not responsible for
RSI or any related diseases caused by prolonged
pipetting.
Cat. No. Colour
Code
Channels Volume
Range/µl
Increment
/µl
Tip
/µl
Safe-Cone Filters
50 pcs/bag
Standard Plus
725010 Grey 1 0.1-3 0.002 10 - -
725020 Grey 1 0 . 5 -10 0.01 10 - -
725030 Yellow 1 2-20 0.02 200 721014 -
725050 Yellow 1 10-100 0.1 200, 350 721008 721018
725060 Yellow 1 20-200 0.2 200, 350 721007 721017
725070 Blue 1 100-1000 1 1000 721006 721016
725080 Green 1 500-5000 10 5000 721005 721015
725090 Red 1 1-10 ml 20 10 ml 721005 721015
725120 Grey 8 0.5-10 0.01 10 - -
725130 Yellow 8 5-10 0 0.1 200, 350 721008 721018
725140 Orange 8 30-300 0.2 350 721007 721017
725220 Grey 12 0.5-10 0.01 10 - -
725230 Yellow 12 5 - 100 0.1 200, 350 721008 721018
725240 Orange 12 30 - 300 0.2 350 721007 721017
mLINE single- and multichannel pipettes
3
Pipette description
Cap .......................
Operating
button ...................
Tip
ejector .......
Display .............
Volume
lock ..................
Colour
coding...............
Tip ejector
collar ......................
Tip cone....................
ID window
and tag
Finger
support
........ Handle
... Calibration
nut
Safe-Cone
....... Filter
Tip cone ...............
........ Tip cone
housing
Tip
........ ejector bar
........ Safe-Cone
Filter
4
The special tip cone design of the pipette offers
the possibility to use replaceable Safe-Cone
Filters to help prevent the contamination and
damage of the pipette. The operating button of
the mLINE allows safe and easy filter ejection. The
pipette uses disposable tips.
3. Sartorius pipette tips
Sartorius pipette tips are recommended for
use with mLINE pipettes. The use of fully
compatible tips will ensure the maximal pipetting
accuracy and precision, and thereby guarantee
the performance specifications given for the
pipette. Sartorius tips are made of pure virgin
polypropylene and manufactured in protected
clean room conditions.
Sartorius offers a full range of standard Optifit,
and SafetySpace™ Filter tips. Sartorius nonfilter tips are available in trays, bulk packages
and space-saving refill systems. Sartorius nonfiltered tips and trays are autoclavable at 121°C
(252F), 20 min, 1 bar (15 p.s.i). All Single Tray
Racks and clean Refill Packs are certified RNase,
DNase and endotoxin-free.
For more information, please go to www.
sartorius.com or contact your local Sartorius
support.
4. Unpacking
The mLINE pipette package contains the following
items:
1. Pipette
2. Tip
3. Caps for colourcoding
4. Calibration tool (also tube and ID window
opener)
5. Pipette holder
6. Identification tags
7. Safe-Cone Filters (pipettes >10 µl)
8. Grease
9. Instruction manual
10. Performance certification in accordance
with ISO 8655-6
Please check that all items are included and that
no damage has occurred during shipment.
Loading...