How it Works
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eLINE
User Manual
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Sartorius eLINE User Manual

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eLINE® Electronic Pipette
User Manual
Contents
1. Intended Use .................................................................................................................................. 2
2. eLINE® Electronic Pipettes ........................................................................................................2
3. Unpacking ....................................................................................................................................... 4
4. Pipette Description ...................................................................................................................... 5
5. Charging of the Pipette ............................................................................................................. 9
5.2. Charging Directly ............................................................................................... 10
5.3. Electrical Specifications .................................................................................. 11
6. Programming Principle.............................................................................................................11
6.1. Programming Map .............................................................................................13
6.2. Speed Adjustment .............................................................................................14
7. Pipette modes ..............................................................................................................................14
7.1. Super Pipetting (SP), Pipetting mode (P) and pipetting with
mixing (P+MIXING) ............................................................................................15
7.2. Reverse pipetting (rP) ....................................................................................... 16
7.3. Pipetting with cycle counter (P COUNT) ................................................... 17
7.4. Manual Pipetting (P MANUAL) ..................................................................... 18
8. Multi-modes ................................................................................................................................. 19
8.1. Multi-dispensing (d) ..........................................................................................20
8.2. Diluting (dd) and Diluting With Mixing (dd+MIXING) ..........................21
8.3. Sequenced Dispensing (Sd) ............................................................................23
8.4. Multi-aspirating (SA) ........................................................................................ 24
8.5. Automatic Dispensing (Ad) .............................................................................25
9. Prog Mode .....................................................................................................................................26
9.1. Store operating mode to storage locations .............................................27
9.2. Recall stored mode from the storage location ....................................... 27
10. Pipetting Recommendations ..................................................................................................28
10.1. Dispensing With Blow-out .............................................................................28
10.2. Dispensing Without Blow-out.......................................................................28
10.3. Other Recommendations ................................................................................28
11. Maintenance ................................................................................................................................29
11.1.1 Cleaning the Outer Surface of the Pipette (daily) ................................. 29
11.1.2. Cleaning the Lower Part of a Single Channel Pipette
(every three months) .......................................................................................30
11.2.1 Cleaning and Autoclaving the Lower Part ................................................30
11.2.2. Sterilizing ..............................................................................................................31
11.2.3. Autoclaving .......................................................................................................... 32
11.2.4. UV Sterilization ...................................................................................................32
11.3. Battery replacement ......................................................................................... 32
11.4. Storage ...................................................................................................................33
12. Performance Testing .................................................................................................................33
12.1. Recalibration........................................................................................................35
13. Troubleshooting ..........................................................................................................................37
14. Warranty Information ..............................................................................................................39
15. Recycling Instruction (WEEE) ...............................................................................................40
16. Performance Specifications ...................................................................................................40
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1. Intended Use
Sartorius eLINE® Electronic Pipettes are intended, designed and manufactured for dispensing liquids in a variety of applications, and are to be used in combination with Sartorius Optifit Tips or SafetySpace Filter Tips. The Sartorius eLINE® Electronic Pipette and tip combination, fall within the scope of in-vitro diagnostics, and can be used as a diagnostic medical device in related applications. Thereby, Sartorius electronic pipettes and Sartorius tips fulfil the relevant demands of the Directive 98/97/EC of the European Parliament.
The electronic pipette product range covers a volume range of 0,1 µl to 5 ml. It is recommended that Sartorius Optifit Tips or SafetySpace Filter Tips are used with Sartorius pipettes to ensure optimum compatibility and performance.
2. eLINE® Electronic Pipettes
The eLINE® electronic pipettes offer you the specialised technology and innovations in the field of the hand held liquid handling devices. The attractive and ergonomic design of the eLINE® pipettes together with electronic tip ejection operation take the effort out of pipetting and reduce the risk of repetitive strain injuries (RSI) that are common in mechanical pipetting.
The microprocessor controlled system and the superior construction of the eLINE® pipettes enables maximum liquid handling performance with high levels of accuracy and precision. The microprocessor controlled piston movements reduces the possibility of human error and thus also contamination of the pipette interior. The tip cone design of the eLINE® pipettes offer the possibility to use replaceable Safe-Cone Filters to help prevent contamination and damage of the pipette.
The eLINE® electronic pipettes offers a comprehensive range of liquid handling protocols, typically required by laboratories currently. The simple keyboard and easy-to­read display together with user-friendly operation and programming allows these tasks to be performed quickly and effortlessly. The eLINE® pipettes operate on the air displacement principle and use disposable tips.
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2.1. eLINE® Single, 8 and 12 Channel Pipettes
Order Code Incl. Charger
Order Code Excl. Charger*
Channels Volume
Range
(µl)
Incre­ment
(µl)
Optifit Tips***
(µl)
Safety­Space Filter Tips*** (µl)
Safe-Cone Filters Standard Plus
730011 730010 1
0,1-5 0,05 10 10 – –
730021 730020 1
0,2-10 0,05 10 10 – –
730041 730040 1
5-120 0,50 200
350
120 721008 721018
730061 730060 1
10-300 1,00 350 300 721007 721017
730081 730080 1
50-1000 5,00 1000 1000 721006 721016
730101** 730100** 1
100-5000 10,0 5000 – 721006 721016
730321 730320 8
0,2-10 0,05 10 10 – –
730341 730340 8
5-120 0,50 200
350
120 721008 721018
730361 730360 8
10-300 1,00 350 300 721007 721017
730391 730390 8
50-1200 5,00 1200 1200 721006 721016
730421 730420 12
0,2-10 0,05 10 10 – –
730441 730440 12
5-120 0,50 200
350
300 721008 721018
730461 730460 12
10-300 1,00 350 300 721007 721017
730491 730490 12
50-1200 5,00 1200 1200 721006 721016
Pipettes are supplied with a universal charger (EU, UK, US | JPN, AUS, KOR and CHN plugs) * Pipettes excluding a charger were discontinued as of 1st January, 2016.
** NOTE: Minimum volume in P-mode is 500 µl. 100 µl is possible in multi-dispensing (d) mode.
***
NOTE: Low Retention Tips are available in volumes up to
1200 µl.
2.2. Sartorius Pipette Tips
Sartorius pipette tips are recommended for use with eLINE® pipettes. The use of fully compatible tips will ensure the maximal pipetting accuracy and precision, and thereby guarantee the performance specifications given for the pipette. Sartorius tips are made of pure virgin polypropylene and manufactured in protected clean room conditions.
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Sartorius offers a full range of standard Optifit, and SafetySpace Filter and Low Retention tips. Sartorius non-filter tips are available in trays, bulk packages and space-saving refill systems. Sartorius non-filter tips and trays are autoclavable at 121°C (252F), 20 min, 1 bar overpressure (15 p.s.i). All Single Tray Racks and clean Refill Packs are certified RNase, DNase and endotoxin-free.
For more information, please go to www.sartorius.com or contact your local Sartorius support.
3. Unpacking
The eLINE® electronic pipettes may be delivered with or without an AC-adaptor, depending from the type of order. However, all eLINE® pipette packages contains:
- Pipette
- Tip
- Safe-Cone Filters and Filter Forceps (>10 µl)
- Grease (in single-channel units only)
- Instructions for use
- Performance certification in accordance with ISO 8655-6
Please check that all items are included and that no damage has occurred during shipment.
NOTE: The battery is protected against discharging
during storage with a plastic protector. BEFORE USE PLEASE REMOVE THE PLASTIC PROTECTOR UNDER THE BATTERY COVER.
NOTE: The pipette is supplied with discharged battery.
It is recommended to recharge the pipette for at least 12 hours before initial use.
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Battery Cover
Charging
Contacts
Reset Button
Finger Hook
Tip Cone
(5000 µl)
Tip Ejector
Assembly
(5000 µl)
Display
Keyboard
Tip Ejection Buttons
Start Button
Handle
Handle Locking Collar
MCP Connecting Collar
Tip Ejector Collar
(10 µl –1000 µl)
Tip Cone Housing
Tip Ejector Bar
Tip Cone(s)
(excl. 5000 µl)
Safe-Cone Filter
Operating Mode Symbol
Mode Range Aliquots/Counter Volume
Speed in/out Battery Symbol
Aspirating/Dispensing direction
Display:
4. Pipette Description
4.1. Display and Controls
The control and programming of the eLINE® pipette is performed using the display, keyboard and operating buttons.
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NOTE: When the pipette is not connected to the
charging unit the pipette/display will automatically switch off after 10 minutes of non-use. Pipette can be switched on by pressing any key.
4.2. Reset Button
To reset the pipette, press the reset button on the battery cover. The display shows RESET and PRESS TIP in a blinking form and pressing the tip ejector button will reset the pipette to the first programming level in PIPET mode.
During the reset function the pipette automatically determines the home position for the piston and tip ejector by running the piston up and down. During the reset function the display shows AUTO TEST and the completed reset function is confirmed by showing the text DONE on the display.
NOTE: The pipette must never be reset with the tip
attached! The attached tip can induce an error in home position determination. However, if resetting is accidentally done with the tip attached reset the pipette again without the tip attached.
4.3. Start Button
The start button triggers the aspiration and dispensing operations according to selected operating mode. Only a soft touch is required to operate the button. If the start button is held down during dispensing, the piston will stop in the lowest position until the button is released. In the operating modes with automatic blow-out (P, dd) the piston will automatically return to the home position after the start button is released.
If the start button is held down in the reverse pipetting mode or during the dispensing of the last aliquot in the multi-dispensing modes the piston direction symbol will change within one second and the pipette will be ready to aspirate the next sample when the start button is released.
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Keyboard:
Select Arrow up/down Enter
- Press to select - Press to select - Press to confirm the mode range or the operating the selections or to activate/confirm mode or to make setting changes setting changes setting changes (volume, aliquots, speed, etc.)
Operating buttons:
Start button Tip ejection buttons Reset button
- Triggers all piston - Press to remove tip - Press to reset the movements pipette
Mode ranges and operating mode symbols:
PROG
PROG 1-6
= 6 storage
locations
MULTI
d = multi-dispensing dd = diluting dd+
MIXING = diluting
with mixing Sd* = sequencial
dispensing SA = multi-aspirating Ad = automatic
multi-dispensing *Not available in eLINE® 5 µl
PIPET
SP* = super pipetting P = pipetting rP = reverse
pipetting P+MIXING = pipetting with mixing P COUNT = pipetting with cycle counter P MANUAL** = manual
pipetting *Only available in eLINE® 5 µl
**Not available in multichannels
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4.4. Tip Sealing and Electronic Tip Ejection
The pipette tips are recommended for use with eLINE® pipettes. The eLINE® multichannel pipettes are provided with a spring loaded OPTILOAD tip cones for optimal tip sealing.
For easy and effortless tip ejection all eLINE® electronic pipettes are equipped with the electronic tip ejection feature. There are two tip ejection buttons conveniently placed on the either side of the start button to ensure easy reach for both right and left handed users.
Tip ejection: When the pipetting cycle is completed, either of the buttons can be depressed to eject the tip.
NOTE: The tip ejection function is frozen during the
pipetting cycle and can only be activated after the tip is emptied. If needed the pipetting cycle can be interrupted by pressing the enter button. The display will show E (E=empty) and the tip is then emptied by pressing the start button.
4.5. Safe-Cone Filters
The tip cone design of the eLINE® pipettes (>10 µl) allows the use of Safe-Cone Filters in the tip cones. These removable filters prevent liquids and liquid vapours from entering the pipette and thus protects the pipette from cross contamination and damage.
The Safe-Cone Filters are available as Standard or Plus versions. It is recommended to use the Standard Filter for general applications and the Plus Filter for more demanding applications. The filters need to be changed regularly. The interval for filter changing is application dependant but the recommendation is to change the filter daily (after 50 – 250 pipetting cycles) and always in case of over-aspiration. The filter forceps should be used to avoid touching the soiled filters by hand. (See Chapter 2.1. for ordering information.)
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5. Charging of The Pipette
The battery is protected against discharging during storage with a plastic protector. BEFORE USE PLEASE REMOVE THE PLASTIC PROTECTOR UNDER THE BATTERY COVER. However, should the battery become discharged during storage and recharging is required, a battery symbol will be observed in the bottom right corner of the display.
The eLINE® pipettes should be charged using the original Sartorius Charging Stand or Charging Carousel. However, the eLINE® also features direct charging by using the eLINE® AC-adaptor.
NOTE: If the battery is fully discharged, the display
will be empty and there will be no response from the operating buttons. After a few minutes on charge, the battery symbol will be displayed and the + and – symbols will continue to blink until the battery is fully charged.
5.1. Charging with the Sartorius Charging Stand and Carousel
The charging stands and carousels are bench top charging units. The charging stand accommodates one eLINE® pipette and the charging carousel four eLINE® pipettes. The stands and carousels operate through the metal contacts placed in the sides of the pipette and in the head of the charging units.
Before operating the AC-adaptor must be connected to the socket at the rear of the charging stand or at the side of the charging carousel as well as to the electrical outlet. Before connecting the AC-adaptor to the electrical outlet, check that its voltage settings corresponds to the voltage settings of your electrical outlet.
Cat. No. Product 730981 Charging Stand for one pipette 730991 Charging Carousel for 4 pipettes
With universal AC-adaptor (Euro, USA/JPN, UK, AUS, KOR and China plugs)
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1. Check that the battery protector is removed and
the battery cover is properly closed.
2. Place the pipette into the charging unit. Make
sure that the metal contacts at the rear of the pipette are properly fitted to the tracks of the metal contacts in the top of the charging unit.
3. When charging, the battery symbol will be
displayed with the + and – symbols flashing. The charging time for completely discharged batteries is 4 hours.
4. When the battery is full the battery symbol
disappears from the display. If display shows RESET and PRESS TIP, press the tip ejection button to reset the pipette, the pipette will then be ready for use.
NOTE: Never reset the pipette with the tip attached
as the attached tip can induce an error in the home position determination of the piston and tip ejector collar!
NOTE: When the pipette is not in use it is recommended
to keep the pipette in the charging unit. This ensures that the battery is kept charged and the pipette is ready for use.
5.2. Charging Directly
The AC-adaptor supplied with the eLINE® pipette or single charging stand can be used for the charging of the eLINE® electronic pipette. The socket for the AC­adaptor is situated on the back of the pipette.
1. Before connecting the AC-adaptor to the electrical
outlet, check that its voltage settings corresponds to the voltage settings of your mains electrical outlet.
2. Check that the battery protector is removed and
the battery cover is properly closed.
3. Connect the AC-adaptor to the mains electrical
outlet as well as to the socket at the back of the pipette.
4. The charging time is 12 hours for a completely
discharged battery.
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NOTE: It is possible to continue pipetting while the
eLINE® pipette is charging, connected to the AC­adaptor. With a fully discharged battery a 30 minute initial charging is required before continuing the pipetting.
5.3. Electrical Specifications
Battery
- Rechargeable NiMH battery pack with protection circuit
- 4.8 V, 500 mAH
- Charge empty batteries for a minimum of 12 hours
AC-adaptor for Charging Stand or direct charging
- Input voltages and main plug according to local requirements
- Output voltage 7,5V DC, 800 mA
AC-adaptor for Charging Carousel
- Input voltages and main plug according to local requirements
- Output voltage 9 V DC, 2000 mA
6. Programming Principle
The eLINE® pipettes features 10-11 different operating modes and six different storage locations for frequently used modes with user-selected settings. The operating modes are divided in to three Mode Ranges (PIPET, MULTI, PROG) and each Mode Range includes the following operating modes:
Mode Range: Operating Modes: PIPET SP, P, rP, P+MIXING, P COUNT, P MANUAL* MULTI d, dd, dd+MIXING, Sd, SA, Ad PROG 1, 2, 3, 4, 5, 6 (=storage locations)
Programming is performed by using the Select, Enter and arrow keys. The mode range is selected with the Select key, the desired operating mode is then selected with the arrow key and selection confirmed with Enter key.
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The setting changes (volumes, aliquots, etc.) are made with arrow keys and confirmed with either Select or Enter key. In the shortcut programming the changes are confirmed with the Enter key. In the shortcut programming the speed adjustment is not possible. The use of the Select key in confirming the setting changes enables also the speed adjustment.
NOTE: When the programming or pipetting cycle is
completed it is possible to make the setting changes inside the operating mode simply by pressing the arrow key and confirming with Enter or Select.
The eLINE® pipette includes also six storage locations (Prog 1–6). The operating mode with user-selected settings can be stored in to the storage location under PROG mode for future recalls. After completing the setting changes of the selected operating mode simply press Enter and Select keys simultaneously, choose the storage location (PROG 1-6) with arrow key and press Enter. The stored operation mode can now be recalled from the PROG mode.
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or
or
Repeat until
programming
is complete
or
PIPET MULTI PROG
SP* d PROG 1
P dd PROG 2
rP dd + MIXING PROG 3
P + MIXING Sd*** PROG 4
P MANUAL** SA PROG 5
P COUNT Ad PROG 6
To change all settings To change settings When pre-stored including speed: excluding speed: Prog 1–6 was chosen
Start pipetting
Store the
operating mode with Change selected settings with settings to Prog 1–6
+
Start pipetting +
Start pipetting Start pipetting
*SP is available only in eLINE® 5 µl **P MANUAL is not available in multichannel models ***Sd is not available in eLINE® 5 µl
6.1. Programming map
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6.2. Speed Adjustment
There are 9 speeds available for both dispensing and aspiration. The speed is indicated in the bottom left hand corner of the display. The default speed is 5 for all operating modes.
Speed can be adjusted during the programming by completing the setting changes with the select key. In every operating mode the speed adjustment is the last programming step. After adjusting the “speed out” the programming is completed with enter or select key and the pipette is then ready for operation.
NOTE: The P
MANUAL mode includes 5 different
dispensing and aspirating speeds.
7. Pipette Modes
The mode range PIPET includes 6 different operating modes.
Symbol Description SP Super Pipetting performs pipetting
with super blow-out function enabling dispensing of very small volumes from the air. SP-mode is available only in eLINE® 5 µl pipette
P Pipetting with automatic blow-out rP Reverse pipetting without blow-out
P + MIXING Pipetting with blow-out followed
with mixing function
P COUNT Pipetting with blow-out and
displayed cycle counter
P MANUAL Manual pipetting with on-line
volume display
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7.1. Super Pipetting (SP), Pipetting mode (P) and Pipetting With Mixing (P+MIXING)
1. Press to display PIPET
2. Scroll with
until the symbol SP, P or P+MIXING
is displayed.
3. Confirm by pressing
4. Select the desired pipetting volume by using the
NOTE: When holding down the the volume display
starts to scroll faster.
5. Press
to confirm selection and to display the
speed in or press
and continue from Step
10.
6. Select the speed in with
(1= Min and 9 =
Max)
7. Press
or to confirm selection and to
display the speed out
8. Select the speed out with
(1= Min and 9 =
Max)
9. Press
or to confirm selections
10. Position the tip to aspirate and press the START button
11. Position the tip to dispense. Press the START button
to dispense the sample. The tip is emptied with blow-out and the piston returns automatically back to the home position.
NOTE: When the START button is held down during
dispensing the piston will stop in the lowest position. When the start button is released the piston will automatically return to the home position.
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To mix:
1.
Position the tip in to the solution, then press and hold the START button
.
Mixing is performed automatically as long as the
START button
is held down.
2. Position the tip to dispense. Press START button twice
NOTE: The mixing is performed with about 70% of the
total volume.
7.2. Reverse Pipetting (rP)
A selected volume plus and excess is aspirated into the tip. After dispensing the selected volume the excess volume remains in the tip and is discarded.
1. Press
to display PIPET
2. Scroll with
until the symbol rP is displayed.
3. Confirm by pressing
4. Select the desired pipetting volume by using
NOTE: When holding down the volume display
starts to scroll faster.
5. Press
to confirm selection and to display the
speed in
or press
and continue from Step 10.
6. Select the speed in with
(1= Min and 9 = Max)
7. Press
or to confirm selection and to
display the speed out
8. Select the speed out with
(1= Min and 9 =
Max)
9. Press
or to confirm selections
10. Position the tip to aspirate and press the START button
11. Position the tip to dispense. Press the START button
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12. Position the tip to discard excess liquid and press the START button twice
NOTE: It is also possible to continue to aspirate and
dispense the same volume without the empty function. To continue, keep the START button depressed during dispensing and within one second the direction arrow will change. Continuing to hold the START button down, place the tip into the liquid again and then release the START button to aspirate the next sample.
7.3. Pipetting With Cycle Counter
(P COUNT)
The eLINE® performs pipetting with blow-out and the cycle counter function. The cycle counter counts up to 99 pipettings and then returns back to 0. It is possible to choose the cycle number to begin with. It is also possible to change the cycle number or reset the counter during the pipetting by using the arrow keys.
1. Press
to display PIPET
2. Scroll with
until the symbol P and COUNT is
displayed.
3. Confirm by pressing
4. Select the desired pipetting volume by using the
NOTE: When holding down the volume display
starts to scroll faster.
5. Press
or to confirm selection.
6. Cycle counter will show 1. Use
to select the
sample number you want to begin with (0-99).
7. Press
to confirm the selection and to display
the speed in
or press
and continue from Step 12.
8. Select the speed in with
(1= Min and 9 = Max)
9. Press
or to confirm selection and to
display the speed out
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10. Select the speed out with (1= Min and 9 = Max)
11. Press
or to confirm selections
12. Position the tip to aspirate and press the START button
13. Position the tip to dispense and press the START button
. The tip is emptied with blow­out and the piston returns automatically back to the home position. The pipette is ready for next pipetting.
NOTE: To change the cycle number or to reset the
counter during the pipetting, press the
to display
the desired cycle number and confirm with
.
7.4. Manual Pipetting (P MANUAL)
(Not available in multichannel models)
In the manual pipetting mode the aspiration and dispensing are controlled manually. The aspiration or dispensing continues as long as the START button is held down (within the selected volume range). The aspiration/dispensing direction can be changed during pipetting by using the arrow key. In the manual pipetting mode the display always shows the actual amount of sample in the tip.
1. Press
to display PIPET
2. Scroll with
until the symbol P MANUAL is
displayed.
3. Confirm by pressing
4. Select the desired pipetting volume by using
NOTE: When holding down the volume display
starts to scroll faster.
5. Press
to confirm selection and to display the
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speed in or press or continue from Step 10.
6. Select the speed in with
(1= Min and 5 = Max)
7. Press
or to confirm selection and to
display the speed out
8. Select the speed out with
(1= Min and 5 =
Max)
9. Press
or to confirm selections
10. Position the tip to aspirate and press the START button
NOTE: The aspiration can be interrupted at any time
simply by releasing the START button and continued by pressing the START button again. The aspiration/ dispensing direction can be changed at any time by pressing the arrow key.
11. Press the
to change the aspiraton/dispensing
direction.
12. Position the tip to dispense. Press the START button
13. When the tip is empty (volume reaches 0 µl), within one second the display will show E. Position the tip to empty the tip with blow-out and press the START button twice
.
8. Multi-modes
The mode range MULTI includes 6 different operating modes.
Symbol Description d Multi-Dispensing dd Diluting dd + MIXING Diluting with mixing function Sd Sequenced dispensing SA Multi-aspirating Ad Automatic multi-dispensing
with selected interval
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8.1. Multi-dispensing (d)
The pipette performs repetitive dispensing of a selected volume. The sum of the dispensing aliquots and an automatically selected excess volume is aspirated into the tip. The excess volume is needed to ensure equal operating conditions for each dispensing step.
1. Press
to display MULTI
2. Scroll with
until the symbol d is displayed.
3. Confirm by pressing
4. Select the desired dispensing volume by using
NOTE: When holding down the the volume display
starts to scroll faster.
5. Press
or to confirm the selection and to
display the aliquots
6. Select the desired number of aliquots with
7. Press to confirm selection and to display the speed in or press
and continue from Step 12.
8. Select the speed in with
(1= Min and 9 = Max)
9. Press
or to confirm selection and to
display the speed out
10. Select the speed out with
(1= Min and 9 =
Max)
11. Press
or to confirm selections
12. Position the tip to aspirate and press the START button
. The display shows PRE OUT and arrow
down to indicate the reset function.
13
. Position the tip to discard priming excess and press
the start button
.
14. To dispense, position the tip, press START button
and repeat until the cycle is complete.
21
15. Finally position the tip to discard any remaining excess and press the START button twice
.
NOTE: It is also possible to continue to aspirate
and dispense the same volume without the empty function. To continue, keep the START button pressed during the last dispensing and within one second the direction arrow will change. Keeping the START button depressed, place the tip into the liquid again and then release the START button to aspirate the next sample.
8.2. Diluting (dd) and Diluting With
Mixing (dd+MIXING)
Two different solutions separated with an air gap are aspirated and then dispensed together with an automatic blow-out. The purpose of the air gap is to avoid contamination when aspirating the second volume but it will not prevent the two liquids from mixing in the tip. In dd+mixing the dilution is followed with the mixing function.
1. Press
to display MULTI
2. Scroll with
until the symbol dd or dd+MIXING
is displayed.
3. Confirm by pressing
4. Select the desired diluent volume (volume 1) by using
NOTE: When holding down the volume display
starts to scroll faster.
5. Press
or to confirm the selection
6. Select the desired sample volume (volume 2) with
7. Press to confirm selection and to display the speed in or press
and cont inu e from Step 12.
8. Select the speed in with
(1= Min and 9 = Max)
9. Press
or to confirm selection and to
display the speed out
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10. Select the speed out with (1= Min and 9 = Max)
11. Press
or to confirm selections
12.
Position the tip to aspirate the volume 1 and press the START button
13. With the tip in the air press the START button again to aspirate an air gap
14. Position the tip to aspirate the volume 2 and press the START button
15. Position the tip to dispense and press the START button
To mix:
1.
Position the tip in to the solution, then press and hold the START button
The mixing is performed automatically as long as
the START button
is held down.
2. Release the START button and position the tip to dispense.
3. Empty the tip by pressing the START button twice
NOTE: The mixing is performed with about 70% of the
total volume.
8.3. Sequenced dispensing (Sd)
A series of different volumes of same solution can be delivered in any desired order. The sum of dispensed volumes can not exceed the nominal volume of the pipette.
1. Press
to display MULTI
2. Scroll with
until the symbol Sd is displayed.
3. Confirm by pressing
23
4. Select the number of dispensing steps by using
5. Press or to confirm the selection
6. Select the first dispensing volume with
NOTE: When holding down the volume display
starts to scroll faster.
7. Pres s
or to confirm selection and to
select the next dispensing volume.
8. Press
to confirm the last volume selection to
display speed in or press
and continue from
Step 13.
9. Select the speed in with
(1= Min and 9 = Max)
10. Press
or to confirm selection and to
display the speed out
11. Select the speed out with
(1= Min and 9 =
Max)
12. Press
or to confirm selections
13. Position the tip to aspirate and press the START button
. The display shows PRE OUT and arrow
down to indicate the reset function.
14. Position the tip to discard priming excess and press the start button
15. Position the tip to dispense and press the START button
. Repeat until the cycle is complete.
16. Finally position the tip to discard any remaining excess and press the START button twice
.
NOTE: It is also possible to continue to aspirate
and dispense the same volumes without the empty function. To continue, keep the START button pressed during the last dispensing and within one second the direction arrow will change. Keeping the START button depressed, place the tip into the liquid again and then release the START button to aspirate the next sample.
24
8.4. Multi-aspirating (SA)
The pipette performs consecutive aspiration operations of the programmed volume. This mode allows e.g. emptying a microwell plate, sample pooling and other special applications.
1. Press
to display MULTI
2. Scroll with
until the symbol SA is displayed.
3. Confirm by pressing
4. Select the desired aspirating volume by using
NOTE: When holding down the volume display
starts to scroll faster.
5. Press
or to confirm the selection
6. Select the desired number of aspirations with
7. Press to confirm selection and to display the speed in or press
and continue from Step 12.
8. Select the speed in with
(1= Min and 9 = Max)
9. Press
or to confirm selection and to
display the speed out
10. Select the speed out with
(1= Min and 9 =
Max)
11. Press
or to confirm selections
12. Position the tips to aspirate and press the START button
repeating the action until the cycle is
complete. The total volume is displayed.
13. Position the tips to dispense and press START button twice
.
8.5. Automatic dispensing (Ad)
The pipette performs repetitive dispensing of a selected volume automatically at the specified interval (0.1–9.9 seconds). The sum of the dispensing aliquots and an automatically selected excess volume is aspirated into the tip. The excess volume is needed to ensure equal operating conditions for each dispensing step.
25
1. Press to display MULTI
2. Scroll with
until the symbol Ad is displayed.
3. Confirm by pressing
4. Select the desired dispensing volume by using the
NOTE: When holding down the volume display
starts to scroll faster.
5. Press
or to confirm the selection and to
display the aliquots
6. Select the desired number of aliquots with
7. Press or to confirm selection
8. Select the desired dispensing pace with
(0.1 -
9.9 seconds)
9. Press
to confirm the selection and to display
the speed in or press
and continue from Step
14.
10. Select the speed in with
(1= Min to 9 = Max)
11. P ress
or to confirm selection and to
display the speed out
12. Select the speed out with
(1= Min to 9 = Max)
13. Press
or to confirm selections
14. Position the tip to aspirate and press the START button
. The display shows PRE OUT and arrow
down to indicate the reset function.
15.
Position the tip to discard priming excess and press
the start button
.
16. To dispense, position the tip, press the START button
once and the pipettor dispenses the
aliquots at the specified pace.
17. Finally position the tip to discard any remaining excess and press the START button twice
.
26
NOTE: It is possible to stop the second counter and
interrupt dispensing at any time by pressing the START button. Releasing the START button activates the counter again and dispensing continues.
NOTE: It is also possible to interrupt the dispensing
by pressing the START button and then ENTER key simultaneously. The display will show E and the tip can be emptied by pressing the START button.
NOTE: Furthermore it is possible to continue to
aspirate and dispense the same volume without the empty function. To continue, press the START button immediately after E is displayed and within one second the direction arrow will change. Keeping the START button depressed, place the tip into the liquid again and then release the START button to aspirate the next sample.
9. Prog Mode
The Prog mode includes six different storage locations (Prog 1–6). The favourite operating modes with user­selected settings can be stored to these locations for future recalls.
9.1. Store operating mode to storage
locations
To store an operating mode to Prog 1–6 locations
1. Complete the programming of the selected operating mode as described in paragraphs 6 and 7
2. Press first
and then simultaneously to
display the PROG 1
3. Choose the desired storage location (Prog 1–6) with
NOTE: The display shows simultaneously the mode
symbol of the previously stored mode in the storage location in question.
4. Press
to store the operating mode in to the
selected storage location
27
NOTE: This procedure overwrites the possible
previously stored mode from the selected storage location.
9.2. Recall stored mode from the
storage location
To recall a stored mode from Prog 1–6:
1. Press
to display PROG
2. Scroll with
until the desired storage location
is displayed (1–6)
NOTE: The display shows simultaneously the mode
symbol of the operating mode stored in to the storage location in question.
3. Confirm by pressing
4. The pipette is ready to perform according to the stored mode. Position the tip to aspirate and press START button
.
10. Pipetting
Recommendations
The wide selection of different operating modes makes it possible to perform several different liquid handling tasks with eLINE® pipettes. The operating modes P, P+MIXING, P COUNT, dd, dd+MIXING and SA feature an automatic blow-out and others leave an excess liquid in the tip. The user should follow the recommendations below to ensure optimal performance.
10.1. Dispensing With Blow-out
The automatic blow-out function, in different pipetting and diluting modes, is followed by an immediate return of the piston to the home position. To avoid accidental aspiration of the liquid back into the tip, it is recommended that the dispensing is always performed above the liquid surface.
28
By holding the START button down during dispensing the piston will stop in the lowest position. This allows the tip to be placed under the liquid surface or against the bottom or the wall of the container during dispensing. Once the liquid is dispensed, the tip can be removed from the container and the START button released.
10.2. Dispensing Without Blow-out
The pipette will not perform the blow-out function in modes rP, P MANUAL, d, Ad, and Sd. Therefore it is recommended that dispensing in these modes is always performed with the tip set against the wall or bottom of the container.
10.3. Other Recommendations
- Hold the pipette vertically when aspirating the liquid and place the tip only a few millimetres into the liquid.
- Pre-rinse the tip before aspirating the liquid by filling and emptying the tip for three to five times. This is important especially when pipetting liquids with a viscosity and density greater than water.
- Check that the pipette, tip and liquid are at the same temperature.
- To avoid the contamination, do not rest the pipette on its side especially when the tip attached.
- Change the Safe-Cone Filter regularly.
- Always place the pipette in the charging unit when not in use.
- Never strike the tip cone against the tip tray when mounting the tips as this can damage several internal components.
- Do not use excessive force when mounting the tips as this can hamper the tip ejection.
- Avoid rough handling, light pressure is all that is required when using the keyboard or mounting the tips.
- Do not drop the pipette or charging unit as this may cause excessive shock.
-
Avoid exposing the unit to extreme temperature changes, humidity and dust (operating temperature from 15°C to 40°C and maximum humidity 80%).
29
11. Maintenance
As with any precision instrument, pipettes have many mechanical and electronic parts that are subject to wear and tear. By taking care of your pipette and carrying out regular maintenance and adjustment, its functionality and performance can be ensured. Cleaning is always recommended after possible contamination or after dispensing corrosive or aggressive chemicals.
To avoid pipette and sample contamination and to extend the pipette’s lifetime, Sartorius Safe-Cone Filters (available for pipettes >10 µl) or Sartorius SafetySpace Filter Tips should be used.
Sartorius provides service, maintenance, and calibration services through its global network of service centres. Please contact your nearest service centre for assistance.
The warranty will be voided if the pipette is disassembled or assembled by an unauthorized person.
NOTE: Make sure the pipette has been decontaminated
before you send it for repair or calibration. Be sure to note any hazardous material that the pipette has been exposed to. For more information see 4.3, Sterilizing the pipette.
NOTE: Turn off the pipette prior to cleaning.
NOTE: It is recommended that gloves are always worn when cleaning the pipette.
11.1.1 Cleaning the Outer Surface of
the Pipette (daily)
To clean and decontaminate the outer surface of the pipette, use a disinfectant liquid or mild detergent , for example 70% ethanol, and a soft, lint-free cloth. Gently clean the surface of the pipette with the moistened cloth and wipe it dry. Pay special attention to the tip cone. Always check the chemical compatibility between the pipette materials and the disinfectant or decontaminant liquid.
30
Change the Safe-Cone Filter regularly with the tweezers provided with the pipette.
Do not let liquids enter the inner parts of the pipette.
11.1.2. Cleaning the Lower Part of a
Single Channel Pipette (every three months)
If the pipette is in daily use, it is recommended to clean, decontaminate, and grease it every three months.
NOTE: We recommend sending multichannel pipettes to your local Sartorius service center for cleaning and greasing.
11.2. Cleaning and Autoclaving
The Lower Part of Pipettes
Single-channel pipettes
The lower parts of the eLINE® single-channel pipettes are autoclavable (NOTE: The 5000 µl model does not have parts no. 2, 3, and 5). To clean, decontaminate or autoclave the lower parts of the pipette follow these steps:
Disassembly:
1. Remove the battery pack (1).
2. 10 µl, 120 µl, 300 µl, 1000 µl: Unscrew the tip ejector collar (2) counter-clockwise to remove it.
5000 µl: Unscrew the white tip cone (4) counter-
clockwise to remove it. Release the black tip ejector assembly from the tip cone by turning it counter-clockwise and then pull it out. Remove the tip cone filter if fitted and continue from Step 4.
3. Unscrew the tip cone holder (3) counter­clockwise and remove the tip cone holder (3), tip cone (4) and spring (5). Remove the Safe-Cone Filter if fitted.
4. Unscrew the exposed piston (6) counter­clockwise and remove it.
Reassembly:
1. Screw the piston (6) clockwise into position.
2. 10 µl, 120 µl, 300 µl, 1000 µl: Place the spring (5) around the piston.
2
3
4
5
6
1
31
5000 µl: Attach the white tip cone by screwing it clockwise. Place the tip ejector assembly to the tip cone and attach by turning it clockwise. Replace the Safe-Cone Filter with the tool provided and continue from Step 5.
3. Push the tip cone (4) into position and attach it by screwing the tip cone holder (3) back. Replace the Safe-Cone Filter with the tool provided.
4. Attach the tip ejector collar (2) by screwing it clockwise.
5. Replace the battery pack (1). Reset the pipettes and press the start button several times to test the movement. Test the tip ejection operation.
NOTE: When reassembling the pipette do not over-
tighten the parts.
Cleaning
To clean the exposed parts use ethanol (70%), or a mild detergent and a soft lint-free cloth. The interior of the tip ejector collar and tip cone can be cleaned with a cotton swab. Rinse with distilled water if needed. Leave the parts to dry. Grease the piston thinly with the grease provided.
Multichannel Pipettes
Cleaning the outer surface (all models):
To clean the exposed parts use ethanol (70%), or a mild detergent and a soft lint-free cloth. Wipe dry.
Cleaning and maintaining the lower parts of all multichannel models
Opening the lower part of a multichannel pipette should only be done by an authorized Sartorius service provider. Please contact your nearest Sartorius service provider or distributor.
11.2.1 Sterilizing
The lower parts of Sartorius electronic pipettes can be sterilized by autoclaving, with UV or by using disinfectant or decontaminant liquids such as 70% ethanol, 60% isopropanol, mild detergent, or similar. Always make sure the materials of the pipette and the disinfectant or decontaminant liquid are chemically compatible.
32
Autoclavable
Not autoclavable
Always follow the autoclaving instructions below.
11.2.2. Autoclaving
The lower parts of Sartorius electronic pipettes are autoclavable, excluding the 1200 µl multichannel model. Please see the autoclaving symbol printed on the lower part of your pipette to ensure the section is autoclavable.
Autoclaving Instructions: Remove the Safe-Cone Filter if attached. Disassemble the lower part:
a. with single channel pipettes, unscrew the tip
ejector collar, tip cone and piston by turning them counter-clockwise. Place these parts in a sterilisation bag
OR
b. with multichannel pipettes, unscrew the
connecting collar counter-clockwise to remove the tip cone housing, and place it in a
sterilisation bag.
Sterilize the parts at 121°C and 1 bar overpressure for 20 minutes. Let the parts cool and dry before reassembling.
11.2.4. UV Sterilization
Sartorius electronic pipettes are made of UV-resistant materials. Sartorius pipettes tolerate temporary exposure to UV radiation. Take note that prolonged or frequent exposure to UV radiation may cause yellowing and brittling of the pipette.
NOTE: The autoclavable parts in the 5000 µl model are
the white tip cone (4), the black tip ejector assembly and the piston (6).
NOTE: Avoid applying too much grease. Use only the
grease provided with the pipette.
NOTE: Before reassemby check that there are no lint
or particles on the surface of the piston.
NOTE: It is always necessary to check the performance
of the pipette after in-house service or maintenance.
33
11.3. Battery Replacement
The design of the eLINE® pipettes enables fast and easy battery replacement. The battery replacement pack includes 4 NiMH batteries fixed to the battery cover. If the batteries do not hold a sufficient charge for proper operation follow these steps for replacement of the battery pack.
NOTE: The battery pack fits in to the compartment
one way only. If the pack is inserted incorrectly the battery cover will not close properly.
NOTE: Only use battery packs supplied by the
manufacturer. The use of any other type of battery will immediately invalidate the warranty!
NOTE: Dispose the used battery pack in accordance of
your local regulations.
1. Hold the pipette in a horizontal position with the
display facing up.
2. Press the two charging contacts simultaneously to
release the battery pack.
3. Place the new battery pack into the battery
compartment and close the battery cover by pressing it lightly.
11.4. Storage
When not in use it is recommended that the pipette is always stored in the eLINE® Charging Stand or Carousel. However, during long storage periods (several months) it is recommended to disconnect the charging stand and carousel from the main outlet.
It is also advisable to remove the battery pack from the pipette. This protects the batteries against discharging during the long storage period.
12. Performance Testing
It is recommended to check the performance of your eLINE® pipettes regularly (e.g. every 3 months) and always after in-house maintenance. However, users should establish a regular testing routine for their
34
pipettes having regard to the accuracy requirements of the application, frequency of use, number of operators using the pipette, nature of the liquid dispensed and the acceptable maximum permissible errors established by the user. (ISO 8655-1.)
Performance test should take place in a draught-free room at 15–30°C, constant to ±0.5°C and relative humidity above 50%. The pipette, tips and the test water should have stood in the test room sufficient time (at least 2 hours) to reach equilibrium with the room conditions. Use distilled or de-ionised water (grade 3). Use an analytical balance with a readability of 0.01 mgs. (ISO 8655-6.)
Weighing:
1. Adjust the desired test volume Vs.
2. Carefully fit the tip onto the tip cone.
3. Fill the tip with test water and expel to waste five
times to reach a humidity equilibrium in the dead air volume.
4. Replace the tip. Pre-wet the tip by filling it once
with the test water and expel to waste.
5. Aspirate the test water, immersing the tip only
2–3 mm below the surface of the water. Keep the pipettor vertical.
6. Withdraw the pipette vertically and touch the tip
against the side wall of the test water container.
7. Pipette the water into the weighing vessel,
touching the tip against the inside wall of the vessel just above the liquid surface at an angle of 30° to 45°. Withdraw the pipette by drawing the tip 8–10 mm along the inner wall of the weighing vessel.
8. Read the weight in mgs (mi).
9. Repeat the test cycle until 10 measurements have
been recorded.
10. Convert the recorded masses (mi) to volumes (Vi)
by multiplying the mass with the correction factor Z (Z-values in the table below):
Vi = mi *Z
11. Calculate the mean volume (
⊽) delivered:
⊽ = (∑V
i
)/10
12. For conformity evaluation calculate the systematic
error es of the measurement:
in µl: es =
⊽ –V
s
Vs = selected test volume
or in %: es = 100 (
⊽ –V
s
)/V
s
35
13. For conformity evaluation calculate the random
error of the measurement:
as standard deviation s = ∑(Vi –
⊽)
2
n –1
n = number of measurement (10)
or as coefficient of variation CV = 100s/
⊽
14. Compare the systematic error (inaccuracy) and
random error (imprecision) to the performance specification values in chapter 14., or to the specifications of your own laboratory.
NOTE: Systematic error is the difference between
the dispensed volume and the selected test volume. Random error is the scatter of the dispensed volumes around the mean of the dispensed volume. (ISO 8655-
1.)
NOTE: Sartorius specifications are achieved in
strictly controlled conditions (ISO 8655-6). Users should establish acceptable maximum permissible errors based on the field of use and the accuracy requirements placed on the pipette (ISO 8655-1).
Z-values (µl/mg): Temp. (°C) Air Pressure (kPa)
95 100 101.3 105
20.0
20.5
21.0
21.5
22.0
22.5
23.0
23.5
1.0028
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0036
1.0028
1.0029
1.0031
1.0032
1.0033
1.0034
1.0035
1.0036
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0035
1.0036
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0036
1.0037
NOTE:
This method is based on ISO 8655.
12.1. Recalibration
The eLINE® electronic pipette’s calibration has been factory checked and certified at 22°C using grade 3 distilled water according to ISO 8655. The pipette’s specifications are guaranteed only with genuine Sartorius tips.
36
NOTE: Sartorius offers an accredited calibration
service. Please contact your local Sartorius representative for further information.
The length of the piston stroke is electronically monitored and the pipette does not normally need recalibration. However, for special applications the eLINE® pipettes can be recalibrated in PIPET modes (excluding P MANUAL) for one selected volume at a time as follows:
1. Press
to display PIPET
2. Scroll with
until the desired mode (P, rP,
P+MIXING or P COUNT) is displayed
3. Confirm the selection by pressing
4. Select the pipetting volume to be recalibrated by
using
5. Press START and simultaneously to enter
the recalibration mode
NOTE: When pressing both buttons, the display will
be blank except for the µl and CAL sign. Releasing the buttons will display the volume to be recalibrated.
6. Use the
to increase or decrease the volume in
incremental steps (±4 increments).
7. Pres s
to confirm the recalibration and to
display speed in or
press
and continue from point 12.
8. Select the speed in with
(1= Min to 9 = Max)
9. Press
or to confirm selection and to
display the speed out
10. Select the speed out with
(1= Min to 9 = Max)
11. Press
or to confirm selections
12. Position the tip to aspirate and press the START
button
13. Position the tip to dispense and press the START
button
37
NOTE: After recalibration the display will show the
operating mode symbol followed with letters CAL to indicate that the mode and the volume in question is recalibrated to give out the new volume.
NOTE: Changing the mode will reset the recalibration.
However, it is possible to store the recalibrated mode to the storage location in PROG mode as described in paragraph 8.
13. Troubleshooting
The eLINE® pipettes have a onboard monitoring program to control the performance of each pipetting action. If any error message appears to the display it means that the pipette has been unable to perform the attempted action properly. In case of an error the display will show Error and PRESS Ent. message. To clear the message please follow these steps:
1. As this procedure will reset the pipette and the tip
ejector please first remove the tip by hand.
2. Recharge the pipette for 15 minutes.
3. Clear the error message by pressing
. The
display will show RESET and PRESS TIP.
3. Reset the pipette by pressing either of the tip
ejector buttons
.
NOTE: Repeated occurrence of error messages
indicates an internal error causing failure of the eLINE® to complete the execution of the pipetting. The eLINE® therefore needs to be returned to the nearest Sartorius Service Centre or your local Sartorius service representative for repair.
38
Symptom Possible cause Solution
Droplets left inside the tip Unsuitable tip Use original Sartorius
tips
Leakage or pipetted volume too small
Non uniform wetting of the plastic
Attach new tip
Tip incorrectly attached Attach firmly Unsuitable tip Use original Sartorius
tips
Foreign particles between tip and cone
Clean the tip cone, attach new tip
Instrument contaminated
Clean and grease the piston and the tip cone
Insufficient amount of grease on a piston and o-ring
Grease accordingly
Pipette out of established specifications
Instrument damaged Return to your Sartorius
service representative for servicing
Pipette blocked, aspirated volume too small
Liquid has penetrated tip cone and dried
Clean and grease the piston and the tip cone
Tip ejector jammed or moves erratically
Tip ejector collar has been contaminated
Remove and clean ejector collar and tip cone
Continuous error messages
Instrument damaged Return to your Sartorius
service representative for servicing
39
14. Warranty Information
eLINE® electronic pipettes are covered by a warranty for two years against defects in materials and workmanship (exept the batteries). Should your eLINE® pipette fail to function according to specifications at any time, please contact your local Sartorius representative immediately.
ANY WARRANTY WILL, HOWEVER, BE DEEMED AS VOID IF FAULT IS FOUND TO HAVE BEEN CAUSED BY MALTREATMENT, MISUSE, UNAUTHORIZED MAINTENANCE OF SERVICE OR NEGLIGENCE OF REGULAR MAINTENANCE AND SERVICE, ACCIDENTAL DAMAGE, INCORRECT STORAGE OR USE OF THE PRODUCTS FOR OPERATIONS OUTSIDE THEIR SPECIFIED LIMITATIONS, OUTSIDE THEIR SPECIFICATIONS, CONTRARY TO THE INSTRUCTIONS GIVEN IN THIS MANUAL OR WITH OTHER THAN THE MANUFACTURER’S ORIGINAL TIPS.
Each eLINE® electronic pipette is tested before shipping by the manufacturer. The Sartorius Quality Assurance Procedure guarantees that the eLINE® electronic pipette you have purchased is ready for use. Each eLINE® electronic pipette is CE marked, fulfilling the requirements of the EN 55014, 1993/EN 55104, 1995/ISO 13485:2003 and Directive (98/79 EC).
40
15. Recycling Instruction
(WEEE)
In compliance with European Directive, WEEE (2002/96EC) on waste and reducting of hazardous substances in electical and electronic equipments, this device must not be recycled as unsorted municipal waste. Instead this device must be collected separately in accordance the local recycling regulations. The solid bar used in conjuction with the crossed-out wheeled bin indicates that the product was put on the European market after 13 August 2005.
16. Performance
Specifications
The manufacturer's specifications are guaranteed only when the manufacturer's original tips are used. The manufacturer's specifications should be used as guidelines when establishing your own performance specifications in accordance with ISO 8655.
Specifications
Order Code
Channels Volume
Range (µl)
Increment (µl)
Test Volume (µl)
Systematic Error* (%)
Random Error* (%)
730011 1
0,1-5 0,05 5
2,5 0,5
1,20 2,00 12,00
0,70 1,20 8,00
730021 1
0,2-10 0,05 10
5 1
1,00 1,40 4,00
0,40 0,70 2,30
730041 1
5-120 0,50 120
60 12
0,60 0,90 3,00
0,20 0,20 1,00
730061 1
10-300 1,00 300
150 30
0,60 0,60 1,50
0,15 0,20 0,80
730081 1
50-1000 5,00 1000
500 100
0,40 0,60 1,60
0,15 0,20 0,50
730101** 1
100-5000 10,0 5000
2500 500
0,50 0,80 1,60
0,17 0,20 0,40
730321 8
0,2-10 0,05 10
5 1
0,90 1,50 4,00
0,60 0,90 3,50
730341 8
5-120 0,50 120
60 12
0,80 0,80 3,00
0,30 0,35 1,50
730361 8
10-300 1,00 300
150 30
0,70 0,90 3,00
0,20 0,30 1,20
730391 8
50-1200 5,00 1200
600 120
0,90 1,20 2,50
0,30 0,30 0,80
730421 12
0,2-10 0,05 10
5 1
0,90 1,50 4,00
0,60 0,90 3,50
730441 12
5-120 0,50 120
60 12
0,80 0,80 3,00
0,30 0,35 1,50
730461 12
10-300 1,00 300
150 30
0,70 0,90 3,00
0,20 0,30 1,20
730491 12
50-1200 5,00 1200
600 120
0,90 1,20 2,50
0,30 0,30 0,80
All pipettes are supplied with a universal charger (EU, UK, US | JPN, AUS, KOR and CHN plugs) * The listed systematic and random error values are valid for Pipetting (P) mode only and are
achieved under strictly controlled conditions during type tests per ISO 8655. Due to the continuous product development by Sartorius, the systematic and random error values may change without prior notice.
** NOTE: Minimum volume in P-mode is 500 µl. In multi dispensing (d) mode, 100 µl is possible.
Sartorius Biohit Liquid Handling Oy Laippatie 1 FI-00880 Helsinki Finland
Phone +358.755.951 lhinfo.finland@sartorius.com www.sartorius.com
Sartorius Lab Instruments GmbH & Co. KG Weender Landstrasse 94 108 37075 Goettingen Germany Phone +49.551.308.0 Fax +49.551.308.3289
www.sartorius.com
Copyright by Sartorius, Helsinki, Finland.
All rights reserved. No part of this publication may be reprinted or translated in any form or by any means without the prior written permission of Sartorius.
The status of the information, specifications and illustrations in this manual is indicated by the date given aside.
Sartorius reserves the right to make changes to the technology, features, specifications and design of the equipment without notice.
All trademarks are Sartorius property unless otherwise stated. Patents granted or pending.
Publication No. SSUL6018-e161105• Order No. 85037-550-53 • Ver. 11 | 2016 © Sartorius Biohit Liquid Handling Oy
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