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eLINE
User Manual
44 pgs1.2 Mb0

Sartorius eLINE User Manual

eLINE® Electronic Pipette
User Manual
Contents
1. Intended Use .................................................................................................................................. 2
2. eLINE® Electronic Pipettes ........................................................................................................2
3. Unpacking ....................................................................................................................................... 4
4. Pipette Description ...................................................................................................................... 5
5. Charging of the Pipette ............................................................................................................. 9
5.2. Charging Directly ............................................................................................... 10
5.3. Electrical Specifications .................................................................................. 11
6. Programming Principle.............................................................................................................11
6.1. Programming Map .............................................................................................13
6.2. Speed Adjustment .............................................................................................14
7. Pipette modes ..............................................................................................................................14
7.1. Super Pipetting (SP), Pipetting mode (P) and pipetting with
mixing (P+MIXING) ............................................................................................15
7.2. Reverse pipetting (rP) ....................................................................................... 16
7.3. Pipetting with cycle counter (P COUNT) ................................................... 17
7.4. Manual Pipetting (P MANUAL) ..................................................................... 18
8. Multi-modes ................................................................................................................................. 19
8.1. Multi-dispensing (d) ..........................................................................................20
8.2. Diluting (dd) and Diluting With Mixing (dd+MIXING) ..........................21
8.3. Sequenced Dispensing (Sd) ............................................................................23
8.4. Multi-aspirating (SA) ........................................................................................ 24
8.5. Automatic Dispensing (Ad) .............................................................................25
9. Prog Mode .....................................................................................................................................26
9.1. Store operating mode to storage locations .............................................27
9.2. Recall stored mode from the storage location ....................................... 27
10. Pipetting Recommendations ..................................................................................................28
10.1. Dispensing With Blow-out .............................................................................28
10.2. Dispensing Without Blow-out.......................................................................28
10.3. Other Recommendations ................................................................................28
11. Maintenance ................................................................................................................................29
11.1.1 Cleaning the Outer Surface of the Pipette (daily) ................................. 29
11.1.2. Cleaning the Lower Part of a Single Channel Pipette
(every three months) .......................................................................................30
11.2.1 Cleaning and Autoclaving the Lower Part ................................................30
11.2.2. Sterilizing ..............................................................................................................31
11.2.3. Autoclaving .......................................................................................................... 32
11.2.4. UV Sterilization ...................................................................................................32
11.3. Battery replacement ......................................................................................... 32
11.4. Storage ...................................................................................................................33
12. Performance Testing .................................................................................................................33
12.1. Recalibration........................................................................................................35
13. Troubleshooting ..........................................................................................................................37
14. Warranty Information ..............................................................................................................39
15. Recycling Instruction (WEEE) ...............................................................................................40
16. Performance Specifications ...................................................................................................40
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1. Intended Use
Sartorius eLINE® Electronic Pipettes are intended, designed and manufactured for dispensing liquids in a variety of applications, and are to be used in combination with Sartorius Optifit Tips or SafetySpace Filter Tips. The Sartorius eLINE® Electronic Pipette and tip combination, fall within the scope of in-vitro diagnostics, and can be used as a diagnostic medical device in related applications. Thereby, Sartorius electronic pipettes and Sartorius tips fulfil the relevant demands of the Directive 98/97/EC of the European Parliament.
The electronic pipette product range covers a volume range of 0,1 µl to 5 ml. It is recommended that Sartorius Optifit Tips or SafetySpace Filter Tips are used with Sartorius pipettes to ensure optimum compatibility and performance.
2. eLINE® Electronic Pipettes
The eLINE® electronic pipettes offer you the specialised technology and innovations in the field of the hand held liquid handling devices. The attractive and ergonomic design of the eLINE® pipettes together with electronic tip ejection operation take the effort out of pipetting and reduce the risk of repetitive strain injuries (RSI) that are common in mechanical pipetting.
The microprocessor controlled system and the superior construction of the eLINE® pipettes enables maximum liquid handling performance with high levels of accuracy and precision. The microprocessor controlled piston movements reduces the possibility of human error and thus also contamination of the pipette interior. The tip cone design of the eLINE® pipettes offer the possibility to use replaceable Safe-Cone Filters to help prevent contamination and damage of the pipette.
The eLINE® electronic pipettes offers a comprehensive range of liquid handling protocols, typically required by laboratories currently. The simple keyboard and easy-to­read display together with user-friendly operation and programming allows these tasks to be performed quickly and effortlessly. The eLINE® pipettes operate on the air displacement principle and use disposable tips.
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2.1. eLINE® Single, 8 and 12 Channel Pipettes
Order Code Incl. Charger
Order Code Excl. Charger*
Channels Volume
Range
(µl)
Incre­ment
(µl)
Optifit Tips***
(µl)
Safety­Space Filter Tips*** (µl)
Safe-Cone Filters Standard Plus
730011 730010 1
0,1-5 0,05 10 10 – –
730021 730020 1
0,2-10 0,05 10 10 – –
730041 730040 1
5-120 0,50 200
350
120 721008 721018
730061 730060 1
10-300 1,00 350 300 721007 721017
730081 730080 1
50-1000 5,00 1000 1000 721006 721016
730101** 730100** 1
100-5000 10,0 5000 – 721006 721016
730321 730320 8
0,2-10 0,05 10 10 – –
730341 730340 8
5-120 0,50 200
350
120 721008 721018
730361 730360 8
10-300 1,00 350 300 721007 721017
730391 730390 8
50-1200 5,00 1200 1200 721006 721016
730421 730420 12
0,2-10 0,05 10 10 – –
730441 730440 12
5-120 0,50 200
350
300 721008 721018
730461 730460 12
10-300 1,00 350 300 721007 721017
730491 730490 12
50-1200 5,00 1200 1200 721006 721016
Pipettes are supplied with a universal charger (EU, UK, US | JPN, AUS, KOR and CHN plugs) * Pipettes excluding a charger were discontinued as of 1st January, 2016.
** NOTE: Minimum volume in P-mode is 500 µl. 100 µl is possible in multi-dispensing (d) mode.
***
NOTE: Low Retention Tips are available in volumes up to
1200 µl.
2.2. Sartorius Pipette Tips
Sartorius pipette tips are recommended for use with eLINE® pipettes. The use of fully compatible tips will ensure the maximal pipetting accuracy and precision, and thereby guarantee the performance specifications given for the pipette. Sartorius tips are made of pure virgin polypropylene and manufactured in protected clean room conditions.
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Sartorius offers a full range of standard Optifit, and SafetySpace Filter and Low Retention tips. Sartorius non-filter tips are available in trays, bulk packages and space-saving refill systems. Sartorius non-filter tips and trays are autoclavable at 121°C (252F), 20 min, 1 bar overpressure (15 p.s.i). All Single Tray Racks and clean Refill Packs are certified RNase, DNase and endotoxin-free.
For more information, please go to www.sartorius.com or contact your local Sartorius support.
3. Unpacking
The eLINE® electronic pipettes may be delivered with or without an AC-adaptor, depending from the type of order. However, all eLINE® pipette packages contains:
- Pipette
- Tip
- Safe-Cone Filters and Filter Forceps (>10 µl)
- Grease (in single-channel units only)
- Instructions for use
- Performance certification in accordance with ISO 8655-6
Please check that all items are included and that no damage has occurred during shipment.
NOTE: The battery is protected against discharging
during storage with a plastic protector. BEFORE USE PLEASE REMOVE THE PLASTIC PROTECTOR UNDER THE BATTERY COVER.
NOTE: The pipette is supplied with discharged battery.
It is recommended to recharge the pipette for at least 12 hours before initial use.
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Battery Cover
Charging
Contacts
Reset Button
Finger Hook
Tip Cone
(5000 µl)
Tip Ejector
Assembly
(5000 µl)
Display
Keyboard
Tip Ejection Buttons
Start Button
Handle
Handle Locking Collar
MCP Connecting Collar
Tip Ejector Collar
(10 µl –1000 µl)
Tip Cone Housing
Tip Ejector Bar
Tip Cone(s)
(excl. 5000 µl)
Safe-Cone Filter
Operating Mode Symbol
Mode Range Aliquots/Counter Volume
Speed in/out Battery Symbol
Aspirating/Dispensing direction
Display:
4. Pipette Description
4.1. Display and Controls
The control and programming of the eLINE® pipette is performed using the display, keyboard and operating buttons.
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NOTE: When the pipette is not connected to the
charging unit the pipette/display will automatically switch off after 10 minutes of non-use. Pipette can be switched on by pressing any key.
4.2. Reset Button
To reset the pipette, press the reset button on the battery cover. The display shows RESET and PRESS TIP in a blinking form and pressing the tip ejector button will reset the pipette to the first programming level in PIPET mode.
During the reset function the pipette automatically determines the home position for the piston and tip ejector by running the piston up and down. During the reset function the display shows AUTO TEST and the completed reset function is confirmed by showing the text DONE on the display.
NOTE: The pipette must never be reset with the tip
attached! The attached tip can induce an error in home position determination. However, if resetting is accidentally done with the tip attached reset the pipette again without the tip attached.
4.3. Start Button
The start button triggers the aspiration and dispensing operations according to selected operating mode. Only a soft touch is required to operate the button. If the start button is held down during dispensing, the piston will stop in the lowest position until the button is released. In the operating modes with automatic blow-out (P, dd) the piston will automatically return to the home position after the start button is released.
If the start button is held down in the reverse pipetting mode or during the dispensing of the last aliquot in the multi-dispensing modes the piston direction symbol will change within one second and the pipette will be ready to aspirate the next sample when the start button is released.
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Keyboard:
Select Arrow up/down Enter
- Press to select - Press to select - Press to confirm the mode range or the operating the selections or to activate/confirm mode or to make setting changes setting changes setting changes (volume, aliquots, speed, etc.)
Operating buttons:
Start button Tip ejection buttons Reset button
- Triggers all piston - Press to remove tip - Press to reset the movements pipette
Mode ranges and operating mode symbols:
PROG
PROG 1-6
= 6 storage
locations
MULTI
d = multi-dispensing dd = diluting dd+
MIXING = diluting
with mixing Sd* = sequencial
dispensing SA = multi-aspirating Ad = automatic
multi-dispensing *Not available in eLINE® 5 µl
PIPET
SP* = super pipetting P = pipetting rP = reverse
pipetting P+MIXING = pipetting with mixing P COUNT = pipetting with cycle counter P MANUAL** = manual
pipetting *Only available in eLINE® 5 µl
**Not available in multichannels
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4.4. Tip Sealing and Electronic Tip Ejection
The pipette tips are recommended for use with eLINE® pipettes. The eLINE® multichannel pipettes are provided with a spring loaded OPTILOAD tip cones for optimal tip sealing.
For easy and effortless tip ejection all eLINE® electronic pipettes are equipped with the electronic tip ejection feature. There are two tip ejection buttons conveniently placed on the either side of the start button to ensure easy reach for both right and left handed users.
Tip ejection: When the pipetting cycle is completed, either of the buttons can be depressed to eject the tip.
NOTE: The tip ejection function is frozen during the
pipetting cycle and can only be activated after the tip is emptied. If needed the pipetting cycle can be interrupted by pressing the enter button. The display will show E (E=empty) and the tip is then emptied by pressing the start button.
4.5. Safe-Cone Filters
The tip cone design of the eLINE® pipettes (>10 µl) allows the use of Safe-Cone Filters in the tip cones. These removable filters prevent liquids and liquid vapours from entering the pipette and thus protects the pipette from cross contamination and damage.
The Safe-Cone Filters are available as Standard or Plus versions. It is recommended to use the Standard Filter for general applications and the Plus Filter for more demanding applications. The filters need to be changed regularly. The interval for filter changing is application dependant but the recommendation is to change the filter daily (after 50 – 250 pipetting cycles) and always in case of over-aspiration. The filter forceps should be used to avoid touching the soiled filters by hand. (See Chapter 2.1. for ordering information.)
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5. Charging of The Pipette
The battery is protected against discharging during storage with a plastic protector. BEFORE USE PLEASE REMOVE THE PLASTIC PROTECTOR UNDER THE BATTERY COVER. However, should the battery become discharged during storage and recharging is required, a battery symbol will be observed in the bottom right corner of the display.
The eLINE® pipettes should be charged using the original Sartorius Charging Stand or Charging Carousel. However, the eLINE® also features direct charging by using the eLINE® AC-adaptor.
NOTE: If the battery is fully discharged, the display
will be empty and there will be no response from the operating buttons. After a few minutes on charge, the battery symbol will be displayed and the + and – symbols will continue to blink until the battery is fully charged.
5.1. Charging with the Sartorius Charging Stand and Carousel
The charging stands and carousels are bench top charging units. The charging stand accommodates one eLINE® pipette and the charging carousel four eLINE® pipettes. The stands and carousels operate through the metal contacts placed in the sides of the pipette and in the head of the charging units.
Before operating the AC-adaptor must be connected to the socket at the rear of the charging stand or at the side of the charging carousel as well as to the electrical outlet. Before connecting the AC-adaptor to the electrical outlet, check that its voltage settings corresponds to the voltage settings of your electrical outlet.
Cat. No. Product 730981 Charging Stand for one pipette 730991 Charging Carousel for 4 pipettes
With universal AC-adaptor (Euro, USA/JPN, UK, AUS, KOR and China plugs)
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1. Check that the battery protector is removed and
the battery cover is properly closed.
2. Place the pipette into the charging unit. Make
sure that the metal contacts at the rear of the pipette are properly fitted to the tracks of the metal contacts in the top of the charging unit.
3. When charging, the battery symbol will be
displayed with the + and – symbols flashing. The charging time for completely discharged batteries is 4 hours.
4. When the battery is full the battery symbol
disappears from the display. If display shows RESET and PRESS TIP, press the tip ejection button to reset the pipette, the pipette will then be ready for use.
NOTE: Never reset the pipette with the tip attached
as the attached tip can induce an error in the home position determination of the piston and tip ejector collar!
NOTE: When the pipette is not in use it is recommended
to keep the pipette in the charging unit. This ensures that the battery is kept charged and the pipette is ready for use.
5.2. Charging Directly
The AC-adaptor supplied with the eLINE® pipette or single charging stand can be used for the charging of the eLINE® electronic pipette. The socket for the AC­adaptor is situated on the back of the pipette.
1. Before connecting the AC-adaptor to the electrical
outlet, check that its voltage settings corresponds to the voltage settings of your mains electrical outlet.
2. Check that the battery protector is removed and
the battery cover is properly closed.
3. Connect the AC-adaptor to the mains electrical
outlet as well as to the socket at the back of the pipette.
4. The charging time is 12 hours for a completely
discharged battery.
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NOTE: It is possible to continue pipetting while the
eLINE® pipette is charging, connected to the AC­adaptor. With a fully discharged battery a 30 minute initial charging is required before continuing the pipetting.
5.3. Electrical Specifications
Battery
- Rechargeable NiMH battery pack with protection circuit
- 4.8 V, 500 mAH
- Charge empty batteries for a minimum of 12 hours
AC-adaptor for Charging Stand or direct charging
- Input voltages and main plug according to local requirements
- Output voltage 7,5V DC, 800 mA
AC-adaptor for Charging Carousel
- Input voltages and main plug according to local requirements
- Output voltage 9 V DC, 2000 mA
6. Programming Principle
The eLINE® pipettes features 10-11 different operating modes and six different storage locations for frequently used modes with user-selected settings. The operating modes are divided in to three Mode Ranges (PIPET, MULTI, PROG) and each Mode Range includes the following operating modes:
Mode Range: Operating Modes: PIPET SP, P, rP, P+MIXING, P COUNT, P MANUAL* MULTI d, dd, dd+MIXING, Sd, SA, Ad PROG 1, 2, 3, 4, 5, 6 (=storage locations)
Programming is performed by using the Select, Enter and arrow keys. The mode range is selected with the Select key, the desired operating mode is then selected with the arrow key and selection confirmed with Enter key.
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The setting changes (volumes, aliquots, etc.) are made with arrow keys and confirmed with either Select or Enter key. In the shortcut programming the changes are confirmed with the Enter key. In the shortcut programming the speed adjustment is not possible. The use of the Select key in confirming the setting changes enables also the speed adjustment.
NOTE: When the programming or pipetting cycle is
completed it is possible to make the setting changes inside the operating mode simply by pressing the arrow key and confirming with Enter or Select.
The eLINE® pipette includes also six storage locations (Prog 1–6). The operating mode with user-selected settings can be stored in to the storage location under PROG mode for future recalls. After completing the setting changes of the selected operating mode simply press Enter and Select keys simultaneously, choose the storage location (PROG 1-6) with arrow key and press Enter. The stored operation mode can now be recalled from the PROG mode.
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