Sakura Tissue-Tek Prisma Plus Operating Manual

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Tissue-Tek Prisma® Plus

Automated Slide Stainer

Operating Manual

Part Number M01-019E-04 Revison Letter B Revised 2018-02-01

Introduction

The automated slide stainer DRS series "Tissue-Tek Prisma® Plus (hereinafter referred to as the "instrument")" is an instrument that automatically stains paraffin strips, frozen strips, cells and other tissues pasted on slides.

Read this Operating Manual carefully so that you can fully utilize the performance of the instrument based on correct procedures. Since the instrument uses organic solvents, exercise due caution when operating the instrument.

Manufactured for:

Sakura Finetek U.S.A., Inc. Torrance, CA 90501, USA Sakura Finetek Europe B.V., 2408 AV Alphen aan den Rijn, NL Sakura Finetek Japan Co., Ltd. Tokyo, Japan 103-0023

Manufactured by:

Sakura Seiki Co., Ltd. Nagano, Japan

(a)

Table of Contents

Safety Precautions ................................................................................................................................... (e)

Explanation of Terms................................................................................................................................ (p)

Chapter 1 Basics of the System

Installation Method .................................................................................................................................. 1-1

Installing the Instrument ...................................................................................................................... 1-1

Flow of Installation ........................................................................................................................... 1-1

Required Tools ................................................................................................................................. 1-1

Installation Environment .................................................................................................................. 1-1

Checking the Installation Conditions ................................................................................................... 1-2

Cecking the Work Area ........................................................................................................................ 1-3

Transportation ..................................................................................................................................... 1-3

Unpacking Procedure .......................................................................................................................... 1-4

Unpacking the System ..................................................................................................................... 1-4

Removing the Securing Members Inside the Instrument ................................................................ 1-6

Switching the Power-supply Voltage Setting ....................................................................................... 1-7

Checking the Accessories ................................................................................................................... 1-7

Checking the Installation (Securing the Instrument) ........................................................................... 1-9

Installing the parts around the instrument ........................................................................................... 1-9

Installation of Accessories ................................................................................................................. 1-14

Turning on the Power ........................................................................................................................ 1-16

Specification .......................................................................................................................................... 1-17

Name of Each Part ................................................................................................................................ 1-20

Standard Accessories/Options .............................................................................................................. 1-22

Basics .................................................................................................................................................... 1-24

Basics on This Instrument ................................................................................................................. 1-24

Instrument Overview ...................................................................................................................... 1-24

Stain Process Modes ..................................................................................................................... 1-24

User Management by Password ................................................................................................... 1-24

Logon/Logoff Function ................................................................................................................... 1-25

Safety Mechanism of the Instrument ............................................................................................. 1-25

How to Open and Close the Cover/Door ....................................................................................... 1-25

Handling the Touch-panel Display ................................................................................................. 1-26

Various Gateways .......................................................................................................................... 1-27

Relationship of Solution Configurations and Station Numbers ...................................................... 1-29

Explanation of Menu Screens ............................................................................................................... 1-32

Stain Process Menu .......................................................................................................................... 1-32

Explanation of Icons in the Stain Process Menu ........................................................................... 1-33

Utility Menu ........................................................................................................................................ 1-34

(b)

Explanation of Icons in the Utility Menu ....................................................................................... 1-34

Edit Menu ......................................................................................................................................... 1-35

Explanation of Icons in the Edit Menu ........................................................................................... 1-35

System Setup ........................................................................................................................................ 1-36

System Setup .................................................................................................................................... 1-36

Setting Date and Time ....................................................................................................................... 1-38

Entering Year ................................................................................................................................. 1-38

Entering Month .............................................................................................................................. 1-38

Entering Day .................................................................................................................................. 1-38

Entering Hours and Minutes .......................................................................................................... 1-38

am/pm ............................................................................................................................................ 1-38

Setting User IDs, Passwords and Accessible Functions ................................................................... 1-39

Setting an ID .................................................................................................................................. 1-39

Setting a Password ........................................................................................................................ 1-39

Setting Accessible Functions ......................................................................................................... 1-40

Stain Process ................................................................................................................................. 1-40

Utility .............................................................................................................................................. 1-40

Edit ................................................................................................................................................. 1-40

Fume Filter Management .................................................................................................................. 1-41

Whether or Not to Use Fume Filter Management.......................................................................... 1-41

Setting a Time Limit ....................................................................................................................... 1-41

Resetting the Actual Time .............................................................................................................. 1-41

Adjusting the Water Flow................................................................................................................... 1-42

Export Data ........................................................................................................................................... 1-43

Names of Files Exported to a CF Card .......................................................................................... 1-44

Chapter 2 Operating Procedure

Operating Procedure ............................................................................................................................... 2-1

Flow of Stain Process Operation ......................................................................................................... 2-1

Flow of Stain Process Operation ..................................................................................................... 2-1

Preparation for Stain Process ............................................................................................................. 2-2

Opening the Water Supply ............................................................................................................... 2-2

Logging On to the Instrument .......................................................................................................... 2-2

Selecting and Saving a Solution Configuration and Setting Solutions ................................................ 2-3

Setting Slides into Baskets .................................................................................................................. 2-4

Start of Stain Process .......................................................................................................................... 2-5

Operations Permitted during Stain Process ........................................................................................ 2-7

Adding a Basket ............................................................................................................................... 2-7

Setting Priority Start ......................................................................................................................... 2-8

Checking the End Station Status ..................................................................................................... 2-8

Checking the Staining Status .............................................................................................................. 2-9

Menu Operations Permitted during Staining Process ................................................................... 2-10

Pausing and Aborting Stain Process ............................................................................................. 2-11

Removing a Basket during Stain Process ..................................................................................... 2-12

(c)

End of Stain Process ......................................................................................................................... 2-12

Removing Stained Baskets............................................................................................................ 2-12

End of Operation ............................................................................................................................... 2-12

How to Start Stain Process by Specifying the Start and End Steps ................................................. 2-13

Setting a Solution Configuration ........................................................................................................... 2-15

Creating a New Solution Configuration ............................................................................................. 2-15

Saving a Staining Program Name ................................................................................................. 2-15

Setting Up a Solution Configuration .............................................................................................. 2-16

Setting an Edit Solution Configuration ........................................................................................... 2-17

Mix Parameter Setup ..................................................................................................................... 2-20

Enhanced Wash Parameter Setup ................................................................................................ 2-20

Basket Lifting Speed and Water Wash Cycle Time Parameter Setup ........................................... 2-21

Temperature Settings ..................................................................................................................... 2-21

Editing a Solution Configuration ........................................................................................................ 2-22

Copying a Solution Configuration ...................................................................................................... 2-22

Editing a Solution Name .................................................................................................................... 2-23

Creating and Editing a Solution Name .......................................................................................... 2-23

Copying a Solution Name .............................................................................................................. 2-24

Setting a Staining Program ................................................................................................................... 2-25

Creating a New Staining Program ..................................................................................................... 2-25

Saving a Staining Program Name ................................................................................................. 2-25

Creating a Staining Program ......................................................................................................... 2-26

Editing a Staining Program ................................................................................................................ 2-31

Copying a Staining Program ............................................................................................................. 2-32

Checking a Staining Program............................................................................................................ 2-33

How to Use the Bar Code Reader, and It's Function (Options) ............................................................ 2-34

Overview ............................................................................................................................................ 2-34

Applicable Solutions .......................................................................................................................... 2-34

How to Use Sakura Staining Solutions ............................................................................................. 2-34

Editing a Solution Configuration .................................................................................................... 2-34

How to Enter Sakura Solution Bar Code Data............................................................................... 2-35

How to Use Sakura Staining Kit ........................................................................................................ 2-36

Editing a Solution Configuration .................................................................................................... 2-36

How to Enter Kit Component Solution Bar Code Data .................................................................. 2-37

Resetting the Solution Usage Information ......................................................................................... 2-39

Outputting Process Report ................................................................................................................ 2-40

Solution Usage Information Report ............................................................................................... 2-40

Process Report .............................................................................................................................. 2-41

Solution Configuration Report........................................................................................................ 2-42

Barcode History Report ................................................................................................................. 2-43

Deleting a Solution Configuration/Staining Program/Solution Name/Process Report ...................... 2-44

Deleting a Solution Configuration .................................................................................................. 2-44

Deleting a Staining Program .......................................................................................................... 2-45

Deleting a Solution Name .............................................................................................................. 2-45

Deleting a Process Report ............................................................................................................. 2-45

(d)

Action to Be Taken upon Power Outage ............................................................................................... 2-46

Power Recovery Operation ............................................................................................................... 2-46

Action to Be Taken upon Error .............................................................................................................. 2-47

Error Handling ................................................................................................................................... 2-47

Errors with a Number ..................................................................................................................... 2-47

Other Errors ................................................................................................................................... 2-47

Chapter 3 Maintenance and Inspection

Daily Inspection and Cleaning ................................................................................................................ 3-1

Inspection and Cleaning Methods ....................................................................................................... 3-1

Cleaning the Solution Reservoirs and Baskets ............................................................................... 3-1

Cleaning the Drying Stations ........................................................................................................... 3-1

Cleaning the Heating Stations ......................................................................................................... 3-1

Replacing Activated Carbon Filters ................................................................................................. 3-2

Cleaning the Arm Holder and Basket Adapter ................................................................................. 3-2

Cleaning the Water Supply Strainer ................................................................................................ 3-3

Cleaning the Cabinet ....................................................................................................................... 3-3

Cleaning the Wash Station Nozzle .................................................................................................. 3-4

Cleaning the Drain Hose .................................................................................................................. 3-4

Chapter 4 Troubleshooting

Troubleshooting ...................................................................................................................................... 4-1

Error Codes Displayed during System Operation ............................................................................... 4-1

Instrument Conditions and Recovery Actions ..................................................................................... 4-7

(e)

Safety Precautions

Designation of "Instrument Control Manager"

● Operation of the instrument requires expert knowledge of the target application, method of use, and so on. So that the instrument can be used correctly and safely, designate a "Instrument Control Manager."

● When the instrument is delivered, the Instrument Control Manager should receive explanation on the handling of the instrument directly from our sales representative.

* Before using the instrument, read "Safety Precautions" to ensure the correct use of the instrument. * The cautionary instructions provided herein are intended to ensure that the instrument will be used

safely in a manner preventing operator from injury and property damage.

* In this manual, instructions pertaining to different levels of potential hazards are classified as and

indicated under , , and .

The definition of each hazard is defined below.

pertains to a potential hazard where failure to observe the specified instruction may result in death or

serious injury of the operator or other person.

pertains to a potential hazard where failure to observe the specified instruction may result in a serious

injury of the operator or other person.

pertains to a potential hazard where failure to observe the specified instruction may result in damage to

this instrument or other property, or affect the process results.

pertains to an item to pay attention to or other useful information.

* The symbols used on the labels attached to the instrument are explained.

The labels bearing one of the following symbols provide particularly important information you must know in order to ensure safety of the operator, improve work efficiency and protect the instrument from damage. Be sure to check these labels and understand the specified instructions before commencing your work.

A label bearing this symbol specifies an action that must be taken. Always follow the instructions.

A label bearing this symbol specifies a prohibited item. Always follow the instructions.

A label bearing this symbol specifies a precaution. Wrong handling against the instruction may potentially expose the operator to danger or result in damage to the instrument. Always follow the instructions.

A label bearing this symbol is provided near a high-temperature area. Exercise caution against burns caused by high temperatures.

Safety Precautions

(f)

WARNING

● Read the unpacking instructions carefully before unpacking the instrument.

When removing the instrument from the pallet, two or more persons should work together because the instrument weighs 150 kg. When removing the instrument from the pallet this way, indicate so in the shipping document and inspect the cargo for damage. As a rule, the instrument is unpacked/installed by Sakura Finetek or our local representative.

● Those who have experience operating a Sakura Finetek product or products other than the

instrument must also read this manual. Also, those who will be operating the instrument should receive training first.

Sakura Finetek will assume no responsibility for any accident that may result from an accident caused by operating the instrument without reading this manual first. If the instrument is operated in a manner not specified in this document, the protective functions of the equipment may be lost.

● Do not apply unnecessary external forces on the instrument and accessories.

The instrument may malfunction or cause accidents.

● Avoid connecting the instrument to a transformer not manufactured by Sakura Finetek.

When moving the instrument to a region where different voltage is used, contact the Sakura Finetek Technical Support representative or local representative.

● Safety mechanisms installed in the instrument only represent the minimum measures taken

to prevent accidents. The responsibility for safe operation of the instrument lies primarily with the owner of the instrument, and persons in charge who operate the instrument.

● Do not use the instrument, if damaged, even when operating it is possible.

Injury or electric shock may result.

● Should the instrument malfunction, turn off the power and disconnect the power plug from

the power outlet. If processing is still in progress, immediately protect the tissues.

Should malfunction occur, turn off the power and contact the Sakura Finetek Technical Support representative or local representative. Disconnecting the power plug also cuts off the main power supply. When installing the instrument, select a place where the power outlet is not concealed and the power plug can be disconnected any time in case of emergency.

● Do not disassemble or modify the instrument.

The instrument may malfunction or cause accident, thus creating a dangerous situation.

● Only the specified solutions can be used with this instrument.

If any other solution is used, processing may not be performed properly and fire or failure may result.

● Do not wet the instrument.

If areas other than those instructed by "Daily Inspection and Care" get wet, current leak may occur and cause fire or electric shock. If wetted, the board, power switch, CF card and CF card slot, connection parts of various terminals art located on the right side of the instrument may cause instrument failure or data damage. If the terminal connection part LAN or bar code terminal is not used, put a protective cap over it.

Safety Precautions

(g)

WARNING

● A wrong operation may stop or interrupt the tissue processing.

Be careful not to let unauthorized personnel manipulate or tamper with the instrument while operating.

A wrong operation may stop or interrupt the tissue processing. (If the power is turned off by unauthorized personnel, the stain process may be interrupted and the staining problems may occur. Arrange the work environment so that only the operators with expert knowledge can use the instrument. (Also consider using external alarms, etc., just in case.)

● Do not bring fire sources close to the instrument.

The instrument uses organic solvents that may ignite upon contact with fire.

● Do not let foreign matter go inside the instrument.

If water, metal, paper or other foreign matter enters the air inlets (to the fan, etc.), fire, electric shock, injury of the user, instrument failure, etc., may occur. If foreign matter entered the instrument, immediately stop the instrument and contact the Sakura Finetek Technical Support representative or local representative.

● Do not operate the instrument while wearing metal accessories.

Conductors (metal pen, accessories, etc.) worn on your body may contact the instrument and cause

electric shock.

● Do not block the intake/exhaust ports and air inlet around the instrument.

Do not block the intake/exhaust ports (air inlets) with a wall or object or use the instrument in a poorly

ventilated place or with the dustproof covers still attached, because heat will be trapped in the

instrument and cause the capacity of the instrument to drop, while fire, accident, failure, etc., may also

occur due to abnormal overheating.

● Periodically check the power cord and cables and power outlet for damage and accumulation

of dust.

Foreign particle and dust accumulating on the power outlet may cause fire. Clean the power outlet

periodically.

● Do not let tissue with flammable solvent attached go inside the drying station.

Deposited flammable solvent may be heated by the drying station and vaporize, causing fire or

explosion.

● Do not install the instrument in a place used as a living area.

If a common area is used, an exhaust system is required. Provide electrostatic discharge protection in the installation location.

● Install the instrument indoors. Do not place the instrument in a location exposed to direct

sunlight, snow or rain.

This product is specified for indoor use, so do not use it outdoors. Ultraviolet rays and high

temperature may damage the instrument. Never use the instrument while exposing it to snow or rain

because it may cause electric shock or short-circuit.

Safety Precautions

(h)

WARNING

● Unplug the power cord from the power outlet and store it while the instrument is not in use.

For safety, if the instrument will not be used for an extended period of time, clean each part

and store the instrument in a place free from humidity and from dust and foreign matter.

Using the instrument in a humid place, place exposed to oil smoke or steam, or dusty place, may

cause its parts to deteriorate, short-circuit, fire, etc. The installation humidity range is 30 to 85% (non-

condensing). If you wish to install the instrument in a special place, consult the Sakura Finetek

Technical Support representative or local representative.

● If dews are formed on the instrument, do not turn on the power.

If the power is turned on while dews are formed on the instrument, current leak may occur and cause

fire or electric shock. Do not connect the instrument to a power-supply facility until dews have

evaporated and the instrument has become completely dry.

● Do not operate the power plug and switches with a wet hand. When connecting/ removing the

power plug, do so by holding the plug.

Failure to observe these instructions may result in electric shock.

● Be sure to use the supplied power cord. Also, be sure to use a correct power cord that is

suitable for the current capacity of region where the instrument is used.

If an inappropriate power cord or voltage is used, instrument malfunction due to short-circuit, fire or

voltage drop, unexpected accidents or injuries due to electric shock, etc., can occur. The preset

voltage cannot be changed by the user.

● For the power cord, a standard cord specified by local law may have to be used.

If a power cord not specified by the PSE standard, UL/CSA standard or EC standard is required,

contact the Sakura Finetek Technical Support representative or local representative.

● Do not forcibly bend, pull, twist or bundle the power cord and cables or otherwise damage the

power cord and cables.

Fire or electric shock may occur.

● Do not overload the outlet and do not use an extension cord.

Doing so may stop the operation of the instrument due to voltage drop, or fire can occur due to heat

generation.

● This instrument has been designed and tested according to CISPR11 Class A. When the

instrument is used in a home, it may cause electromagnetic interference, in which case

measures to reduce interference may have to be taken.

● Radiant waves may prevent this instrument from operating properly, so do not use the

instrument near a radiation source of strong electromagnetic waves.

● Use the instrument under the conditions (such as voltage within 10% of the rated voltage)

stated in the Specification.

Safety Precautions

(i)

CAUTION

● Do not pinch the drain hose or allow it to slacken.

Water will not be drained properly and overflow from the instrument.

● Set the solution station (tray) in the correct location.

If not, the tissues may not be soaked in the solution and staining problems may occur.

● Store the slides evenly in the basket.

If the slides are stored unevenly, basket movements are affected during the staining operation.

● Before staining is started, confirm that the end station has been set.

If the end station is not set, the basket cannot be put down and will remain gripped. If the next basket

is processed in this condition, the baskets may collide with each other and the tissues may be

damaged.

● Do not touch the moving arm.

The arm should stop automatically when the cover is opened, but it may continue to operate if a

series of operations is still ongoing. Touching the moving arm may result in injury. If you must move

the arm by hand, confirm that the arm has stopped completely and then move the arm with due

caution by following the onscreen messages on the display.

● Do not open the cover or door unless necessary.

Open the cover or door only when required by the operations explained in this manual. If the cover or

door remains open for a long period of time, organic gas may be released from the instrument. Also,

opening the cover or door during staining stops the arm, which may cause the tissues to dry up or

extend the staining time and eventually affect the stained quality of tissues.

● Be careful not to pinch your hand when opening/closing the cover or door.

Be careful not to pinch your hand between the fixed part and moving part of the cover or door or

between the cover and door.

● Do not move the instrument while operating. Move the instrument after removing all solution

bottles, paraffin containers and other components carrying liquid inside.

Moving the instrument while operating may cause the solution or paraffin to leak. Accident or failure may also occur.

● If the straining process is suspended, the process may be negatively affected depending on

the situation. Remember to resume operation.

Prolonged suspension may negatively affect the stain process. Keep the suspension time to the

minimum.

● When the expiration date of the current solution is passed, a message appears to urge

exchanging the solution. Exchange the solution properly in order to maintain the staining

quality.

● The safety data sheet of each solution used can be obtained from the supplier/manufacturer of

the solution. You can also download it from the website of respective supplier/manufacturer of

the solution.

● Do not insert a metal bar or other metal object into the hot air outlet of any drying station or

use a metal product to reset an over temperature protector.

Metal contact may cause electric shock.

Safety Precautions

(j)

CAUTION

● Do not allow any foreign object to be caught between the cover/door and the stainer.

It may cause an unexpected problem.

● Use tap water for the water supply.

Use of water containing pollutants may result in staining problems.

● Confirm that no paper or foreign object is present at the cover or outlet of any drying station.

It may result in insufficient drying.

● Do not touch the drying stations and heating stations while the instrument is operating.

These stations become very hot during operation, so touching them may result in burns.

● Securely connect the water supply hose and drain hose to make sure water will not leak.

If water leaks, the facility may be damaged.

● Before using the instrument, inspect the water supply hose and drain hose to ensure that they

are free from damage and connected properly.

It may cause an unexpected problem.

● Close the water tap after using the instrument.

If the water tap remains open, the water supply hose may accidentally come off due to water

pressure and cause water leaks.

● Clean the water supply strainer regularly. Also, close the supply source water tap before

cleaning.

If the water supply strainer is not cleaned regularly, the water supply volume will drop and washing

problems may occur. Check the flow rate after cleaning. If the water supply strainer is cleaned with the supply source tap open, water will overflow when the strainer is removed.

● Clean the drying station regularly.

If the drying solution is not cleaned regularly, paraffin will accumulate in the pan and cause the temperature to vary, resulting in staining problems.

● Clean the water supply nozzle regularly.

If the water supply nozzle is not cleaned regularly, contaminants may attach to the nozzle and enter

the tissues, causing staining problems.

● Clean the instrument thoroughly after using corrosive solutions.

Some solutions have corrosivity and other aggressive properties that damage the instrument. It is

best to avoid using these corrosive solutions, but if you must use a corrosive solution, remove the

solution from the instrument and store it properly after use and also clean the instrument thoroughly. If

this is not performed, the metal parts will corrode (rust, etc.).

[Corrosive Solutions]

Acids [hydrochloric acid (HCl), hydrobromic acid (HBr), hydrofluoric acid (HF), etc.], staining solutions

and reagent solutions containing hydrochloric acid (Schiff’s reagent, cold Schiff, sulfurous acid

solution, resorcin‐fuchsin staining solution, etc.), oxidizing chlorides [ferric chloride (FeCl3), mercuric chloride (HgCl2), cupric chloride (CuCl2), sodium hypochloride (NaOCl), etc.], tioculfates [sodium tiosulfate (Na2S2O3), etc.], organic acids [formic acid (HCOOH), acetic acid (CH3COOH), bromic acid (C204H2), lactic acid (CH3CH(OH)COOH), etc.]

Safety Precautions

(k)

CAUTION

● When the pull-up speed is increased, more solution will be brought into the next station and

accelerate solution deterioration, resulting in lower staining quality. There are also risks of

tissue contamination due to dropping solution.

● Do not stare at the laser beam from the 2D bar code reader.

For details, refer to the operating manual. The operating manual can be downloaded from the website

of the 2D bar code reader manufacturer.

● Observe the rated water pressure stated in the Specification of the instrument.

If the water pressure is high, water may leak and cause electric shock or the instrument may stop.

● Do not use any CF card other than the one that comes with the instrument. Also, run a virus

check before inserting the CF card.

● Turn off the power before performing cleaning.

Unless otherwise instructed in this manual, always turn off the power and disconnect the power plug

before cleaning the instrument. Do not spill liquid inside the instrument while cleaning it. If cleaner is

used, follow the safety precautions provided by its manufacturer and safety rules set by each test

laboratory.

● Secure the instrument with seismic anchorage (optional) to prevent it from moving/falling in

case of earthquake.

Provide fall prevention measure to prevent the instrument from moving should a disaster occur.

● Do not apply excessive force on the display.

The display may break and cause injury.

● Do not remove the warning/caution labels.

Without these labels, the operators cannot be reminded of the necessary warning/cautionary

information in the day-to-day handling of the instrument, which may result in unexpected problems.

● Do not use damaged accessories (reservoirs, baskets, basket adapters, etc.).

The accessories deteriorate from use over time. If deteriorated accessories are used, unexpected

problems may occur. Check the accessories before use.

● Do not put solution, etc., on the instrument. Also, do not spill solution on the painted surfaces,

caution/warning labels or touch screen.

If solution is spilled over the instrument, the instrument may fail or sustain damage.

● Do not spill solution inside or outside the instrument.

If solution was spilled, turn off the power to the instrument and then quickly wipe off the spilled

solution. The instrument may stop or the facility may be damaged, etc. Also, other solution may be

contaminated. Do not use any contaminated solution.

● Put the lids on other stations when exchanging solution.

If solution is spilled during exchange, other stations may be contaminated. Also, do not use, but

always replace, any contaminated station.

● Do not put any obstacle within the moving range of the arm.

Failure may occur.

Safety Precautions

(l)

CAUTION

● Do not clean the solution reservoirs and baskets using hot water or heat the solution

reservoirs containing solutions in a microwave oven.

Heating the solution reservoirs and baskets using hot water or in a microwave oven may cause the reservoirs/baskets to deform. Severely deformed baskets and solution reservoirs may rub against each other on side faces or at corners, thereby inhibiting the operation.

● Do not use any ultraviolet sterilizing lamp around the instrument.

Ultraviolet light may cause the instrument characteristics to change or deteriorate.

● Fill each solution reservoir with a specified amount of solution.

Failure to do so may cause tissues to dry up or result in staining problems.

● Replace the activated carbon filters in the fume control unit periodically.

Replace the activated carbon filters regularly in order to maintain a better environment around the

instrument. Expired activated carbon filters cannot maintain exhaust gas at an appropriate level of

concentration. When replacing the activated carbon filters, put the lids on the solution stations or

remove the solution stations by considering the possible scattering of activated carbon particles, to

prevent solution contamination. * If the instrument is connected to an external exhaust device, the activated carbon filters need not be

replaced.

● Use dedicated activated carbon filters for the fume control unit.

Use of inappropriate activated carbon filters may cause explosion of powder dust generated from

activated carbon powder.

● Inspect the instrument every 6 months.

Maintain/inspect the instrument periodically so that it operates correctly at all times. For details on

periodic inspection, contact the Sakura Finetek sales representative.

● If the instrument must be moved after installation, contact the Sakura Finetek Technical

Support representative or local representative.

Unexpected accident may occur by relocating the instrument, so contact the Sakura Finetek Technical

Support representative or local representative.

● Use designated consumable parts.

When replacing consumable parts, use those specified in this manual. Use of any other consumable

part may result in malfunction or failure.

● Close the lids of solution reservoirs whenever the instrument is not operating.

If the solution reservoir lids remain open for a prolonged period of time, organic gas may be released

to the room.

● Provide sufficient ventilation around the instrument.

Some of the solutions used in the instrument are toxic/harmful to the human body. Accordingly,

provide sufficient ventilation around the instrument.

● Install the instrument away from other electrical equipment.

Electromagnetic waves and vibration, heat and solution may affect and be affected by other systems

located nearby.

Safety Precautions

(m)

CAUTION

● Provide a proper space around the instrument.

Provide at least 10 cm of clearance around the instrument and make sure the power plug is installed

so that it can be accessed at any time.

● Thoroughly check the installation location before installing the instrument.

Install the instrument on a flat, non-slippery sturdy floor having a sufficient size. If the installation location is tilted or too small, unexpected accidents may occur.

● Do not use the instrument in a place subject to too low or high temperatures.

Use it in a place where the temperature is stable in a range of 10C to 40C.

● Be sure to connect the power plug to a power outlet with grounding bar.

Connect the power plug alone to a power outlet with grounding bar conforming to at least the Class D

grounding specification. If the instrument is not grounded properly, fire or electric shock may result

due to leak current.

● Lightning and power outage may cause the instrument to fail and stain process may not be

able to continue and adversely affect staining. If the power supply is unstable, use an

uninterruptible power supply.

Lightning may cause the instrument to fail and damage the tissues. If the instrument will be used in a

region where lightning damage is a possibility, use an uninterruptible power supply. If lightning has

caused an irreparable damage to the instrument, take out the basket from the instrument and take an

appropriate measure.

● Do not perform stain process if an extended period of power outage is scheduled. Staining

quality may drop. Staining problems may occur depending on when the power outage occurs.

In this case, use a uninterruptible power supply. If you find that the stain process has stopped due to

an unexpected power outage lasting for an extended period of time, remove the baskets from the

instrument and take appropriate action.

● Install the power cord and cables carefully by making sure the cord and cables will not

interfere with the work.

Failure to do so may result in the operator tripping over the power cord and cables and sustaining

injury.

● Securely connect the power cord and power outlet. If the power cord came off or the power

switch was turned off inadvertently, connect the power cord or turn on the power switch

without delay.

If they are loosely connected, heat may be generated at the connected part, voltage drop can occur

and the instrument may not operate normally. Furthermore, unexpected accidents or injuries can

occur. Also, the tissues may be damaged when the power is cut off during processing.

● Handle the power cord with care. Also, be aware of damage caused by mice and other small

animals.

To unplug the power cord from the power outlet, do not pull the cord, but hold the plug and then pull it

out. Inspect the power cord periodically. If any damage is found in the exterior of the power cord, stop

using it and contact the Sakura Finetek Technical Support representative or local representative.

● Before operating the instrument, be sure to check the electromagnetic environment.

Example: Remove electrostatics from the operator and humidify the room.

Safety Precautions

(n)

CAUTION

● If an error (trouble) occurs, staining problems may occur. Immediately resolve the error,

protect the tissues, and contact the Sakura Finetek Technical Support representative or local

representative.

Connect an external alarm to prevent staining problems from happening when the instrument

experiences problems. When introducing an external alarm, contact the Sakura Finetek Technical

Support representative or local representative.

● Conduct a staining test before determining the staining conditions.

● Check the staining program before the operation. If a wrong staining program is used, staining

problems may occur.

A wrong operation may interrupt the stain process or edit the program to a wrong one. If staining is started with a wrong program, staining problems may occur. Read this manual carefully and use the instrument correctly.

● Operating the instrument with an inappropriate temperature setting or time setting (long/short)

may cause staining problems. Conduct a verification test beforehand to confirm that staining

can be performed properly. If you have any question regarding staining programs, contact the

Sakura Finetek Technical Support representative or local representative.

● The allowable concentration limit of each substance in the work environment varies

depending on the air temperature, size of the room, additional load, if any, ventilation efficient

y, etc.

If danger is suspected, the Instrument Control Manager should measure the concentration of the

applicable substance in the area and confirm that the allowable limit in the work environment is not

exceeded. Follow the standard concentration limits specified by the environmental regulation of the

country/region in which the instrument is used.

● Solution may scatter and attach to your body while working. Be sure to wear protective gears while working.

In all operations including stain process, solution exchange, opening/closing of doors, cleaning and daily maintenance, the solutions used may scatter and attach to your body (eye, mucous membrane, skin, mouth, nose, etc.) or you may inhale their gases. Be sure to wear protective gears (gloves, organic solvent mask, goggles) while working.

● If any solution harmful to human health attached to your body or entered your mouth, take an appropriate action according to the SDS of the solution.

Wear protective gears (mask, gloves, goggles) during work in case anything goes wrong. Use protective gears that are resistant to all solutions on the solution list.

● You may accidentally touch an infectious source and operate the instrument with the contaminated hands. Be sure to wear protective gears when operating the instrument.

If you have operated the instrument after handling a pathogen, disinfect the instrument properly. The disinfection method varies depending on the type of pathogen, so choose a method appropriate for

the pathogen. Also, disinfect/sterilize or otherwise properly treat the protective gears used and then dispose of them as infectious waste. For the disposal method, follow the applicable regulation and guideline specified by each institution, country or region.

Safety Precautions

(o)

CAUTION

● Exercise due caution when handling solutions. Follow the relevant regulations and guidelines

in your country or region and take proper measures to protect the operator, such as wearing gloves, mask and/or safety goggles.

Some solutions are toxic or harmful to the human body. Some solutions are very flammable,

combustible, and/or harmful to human body. Use protective gears that are resistant to all solutions on the solution list.

● Sterilize any potentially infectious waste material or infectious medical waste and then

dispose of it according to the applicable regulation or guideline in the country or region.

● When the instrument is used to handle any substance presenting biological hazard, ensure

safety according to the applicable regulation or guideline in the country or region. When performing operations that involve any such substance, also wear gloves, mask, safety goggles and/or other protective gears and perform the operations inside a safety cabinet by taking appropriate measures to protect yourself as the operator.

● Handling of solutions requires expert knowledge on associated hazards due to their toxicity

and volatility, etc.

Appoint a person responsible for solutions and use solutions under the guidance/supervision of the responsible person.

● If any additional requirement on accident prevention or environmental preservation is

specified in the country/region where this instrument is used, follow the instructions under the applicable law/regulation in addition to the content of this manual to ensure compliance with the requirement in the applicable country/region.

● When disposing of used solutions, activated carbon cartridges, consumable accessories

(solution reservoirs, baskets, basket adapters, etc.), follow the applicable regulation in the country or region.

(p)

Explanation of Terms

● Mix

To cause a basket to move up and down inside a solution-filled station before or after the basket is transferred.

● Enhanced Wash

To repeat the process of immersing a basket into a solution and then removing it completely out of the solution inside a station.

● Slide

A slide made of glass on which tissues are pasted.

● Basket

A special staining basket in which slides are set. Two types of baskets, 10-slide type and 20-slide type, are available.

● Batch mode

An operation mode in which the next staining run cannot be started unless the current staining run is completed.

● Continuous mode

An operation mode in which staining runs of different tissues can be started during the current staining run.

● Solution station

A general term referring to both solution reservoirs and wash reservoirs.

● Start station

A solution station in which baskets are set at the start of staining.

● End station

A solution station in which stained baskets are stored.

● Program End (PE) station

A solution station in which baskets are temporarily held when the staining program is aborted in the middle.

Chapter 1 Basics of the System

1-1

Installation Method

Installing the Instrument

This section provides information on determining a location for, and installing the instrument. Installation should be performed by qualified service staff working exclusively with the instrument. The instrument must be installed correctly to ensure proper operation and service. Read this Operating Manual carefully before operating the instrument. Strictly observe all instructions provided in this document.

This is a precision instrument and must be handled with care. If the instrument is handled in a rough

manner or dropped, its internal components may malfunction or get damaged. Always exercise due care when handling the instrument.

Flow of Installation

 Checking the Installation Location and Transporting the Instrument

Details: P. 1-2

• Checking the installation conditions

• Checking the work area

• Transporting the instrument

● Unpacking and Checking the Installation

Details: P. 1-4

• Unpacking and installation

• Installation check

● Setting before Commencing Operation

Details: P. 1-16

• Turning on the power

Required Tools

Name

Size

Q'ty

Transport handle

Phillips screwdriver

ISO No. 2

Adjustable wrench

300 mm or more

Wrench or box wrench

24 mm across flats

Convex ruler (steel tape measure)

Capable of measuring objects of 2.5 m or larger

Level - 1

Cutter - 1

Pipe wrench

200 mm or more

Installation Environment

As is the case with all electronic devices, the instrument should not be installed in places subject to excessive temperature or humidity. Temperature and humidity should be held at constant levels to achieve the highest degree of operating stability. The ambient operating temperature of the instrument is in a range of +10 to +40C. Its ambient operating humidity is in a range of 30 to 85% in relative humidity (non-condensing). Also, locate the instrument, with at least 10 cm away from walls, in a well-ventilated place not exposed to corrosive vapor or extremely high/low temperatures or where the instrument will not come in direct contact with air flow. Install the instrument away from direct sunlight and do not install it proximity to open windows, ovens, open flames, hot plates, radiators, and dry ice baths. The instrument should be installed away from any equipment carrying high voltage or current, such as a large refrigerator or oven. Since the instrument is very heavy, the experiment bench should have sufficient bearing strength, and the instrument should be laid flat. The instrument should be installed near a power supply meeting the power facility requirements (voltage, current) specified in this Operating Manual. Be sure to ground the power outlet and wire the power properly using a dedicated cable.

If the instrument is used in a non-ventilated place, serious health risks may occur to the human body.

Installation Method

1-2

Checking the Installation Conditions

 Checking the installation

location

Confirm beforehand that the location is flat, strong and meets the dimensions of installation location (installation space) shown in the figure on the left. Confirm that no foreign objects that may block the vents of the instrument (such as hanging towels, etc.) are present in and around the installation location. Confirm that the facility-side power supply conforms to the requirements.

 Checking the facility-side power supply

Confirm that the facility-side power supply conforms to the requirements.

The instrument can support the power supplies listed below using the selector switches.

Switching of the voltage setting should only be performed by qualified service staff working exclusively with the instrument.

Facility requirements

• Single-phase 100 VAC±10%

• Single-phase 115 VAC±10%

• Single-phase 230 VAC±10%

50/60 Hz: 15 A or more 50/60 Hz: 15 A or more 50/60 Hz: 7 A or more

Power outlet with grounding bar Power outlet with grounding bar Power outlet with grounding bar

If the above facility requirements are not satisfied (such as when the power supply used is 200 VAC),

the instrument must not be used. If the instrument generates abnormality, the breaker on the facility side may actuate and other equipment may be affected; accordingly, connect each instrument to a dedicated power outlet (including a dedicated breaker on the facility side).

• Specification of the power cord supplied with the instrument

Type of power cord

Applicable standard

Length

For 100/115 VAC

UL/CSA, PSE

Approx. 2 m

For 230 VAC

Cord: CENELEC HD VDE Plug: EN IEC VDE Connector: EN VDE

Approx. 2 m

If the instrument is used in a region where the applicable standard above is not met, provide a different

power cord conforming to the standard in the location of use.

• About grounding Confirm with the person responsible for the facility that the power outlet to be used is Class-D grounded.

 Confirm that a ventilation system, local exhaust system or exhaust port leading to the outside

of the building is available on the facility side.

Although the volumes of volatile solution gases released from the instrument vary depending on the solutions and processing programs used and the environmental temperature, volumes of gases released per unit time are provided below for reference. Based on these values and the volumetric size of the installation location, check if the ventilation rate is sufficient to meet the requirements at the installation location pertaining to the concentrations of substances in the work environment. If no ventilation system is available, use a 38 or 75 exhaust duct adapter (optional) to connect the duct hose to the exhaust port of the instrument, and discharge the exhaust fume to the outside of the building.

Maximum volumes of gases released per unit time (reference)

Ethanol: 3.9×10-3 (m3/hr) Xylene: 4.8×10-4 (m3/hr)

Installation Method

1-3

Checking the Work Area

The dimensions and weight of the instrument in packed state are as follows. Check them beforehand so that sufficient space and safety can be ensured when "transporting, unpacking and installing" the instrument.

"Dimensions and weight in packed state" W: Approx. 1500 mm H: Approx. 930 mm D: Approx. 900 mm Weight: Approx. 210kg

"Instrument dimensions and weight W: Approx. 1250 mm (excluding the display) H: Approx. 630 mm D: Approx. 713 mm Weight: Approx. 150kg

Transportation

When transporting the instrument on a pallet truck, etc., one person should not work alone in order to

prevent instrument damage or accident caused by rear-end collision, dropping of the instrument, etc. When transporting the instrument, place thin iron sheets, etc., to eliminate height gaps on the floor so that the instrument will not receive impact. Pay due attention to safety. Particularly when transporting the instrument on a slope, remember that, because the instrument is heavy, stopping the instrument will not be easy once it began accelerating.

 Transporting the instrument to

the work area

• Transporting on a forklift or pallet truck

When transporting the instrument on a forklift or pallet truck, do so when the instrument is still packed, and insert the fork into the packing pallet as shown in the figure below. Refer to the palletizing size in the figure on the left and check the dimensions of the forklift or pallet truck beforehand.

 Moving to the installation location

1. Unpack the instrument by referring to the next section, "Unpacking Procedure - Unpacking the system," and move the instrument to the installation location.

2. If the instrument can be transported on a pallet truck, etc., move the whole packing pallet to near the installation location.

3. Remove the L brackets securing the instrument to the packing pallet.

4. Screw in the transport handles into the treaded parts on the left and right side faces at the bottom of the instrument. Securely screw in the transport handles all the way.

5. Hold the transport handles to lift the instrument and move it to the installation location.

When using a pallet truck, etc., for transport, confirm that the instrument and packing pallet are

secured together with L brackets, because otherwise the instrument may shift and drop from the packing pallet. When the instrument must be pushed while being transported on a pallet truck, etc., pay attention to where on the instrument to be pushed, because the exterior panels may be deformed or the acrylic cover may be damaged. (Do this by holding the transport handles at the base of the system.) Pay due attention to safety, and particularly when transporting the instrument on a slope, remember that, because the instrument is heavy, stopping the instrument will not be easy once it began accelerating. When lifting the instrument to move, be sure to secure a line of movement or footing.

Inlet

Front Face of the Instrument

Side Face

Inlet

Installation Method

1-4

Unpacking Procedure

How to remove the instrument from the packing box that has been carried in, and remove the protective materials, is explained. To perform each work safely and correctly, be sure to follow the procedure specified herein.

Confirm before unpacking the instrument, that the packing materials have not been damaged due to

external forces applied during transport. Two or more persons should always work together to unpack the instrument. Be careful not to damage/scratch the instrument when unpacking. When taking the accessory box out of the instrument, assume a natural posture.

Unpacking the System

1. Cut the bands with a cutting knife, etc. and remove the cap.

2. Remove the cushion materials put around the instrument, and take out the sleeves and polyethylene bags.

3. Remove the cushion materials inserted into the front cover from both sides, lift the front cover, and take out the cushion materials sandwiched between the front cover and the front door.

Cushion material Cushion material Cushion material

Cap

Sleeve

Ester band

Installation Method

1-5

4. Remove the tape securing the top cover, and take out the cushion materials sandwiched between the cover and the accessory box.

5. Cut the PP band and take out the accessory box.

6. Remove the cushion materials inserted underneath the display arm, and the cushion foams wrapping the instrument.

Cushion material

Tape

Accessory box

PP band

Cushion material

Cushion foam

Installation Method

1-6

Removing the Securing Members Inside the Instrument

1. Remove the cushion materials sandwiched between the robot arm and the rail.

2. Remove the tape securing the holder, and remove the cushion materials inserted underneath the holder.

3. Remove the reinforcing cushion materials on the tray, and take out the cushion foam sheet and PP band.

4. Remove all 3-position and 4-position reservoir trays stored in the system, and remove the tape securing the cushion foam sheet and the water supply nozzle.

Tape

Cushion material

PP band

Cushion foam sheet

Sheet securing the water supply nozzle

Installation Method

1-7

Switching the Power-supply Voltage Setting

This instrument supports multiple voltages, but the setting on the instrument side must be switched

according to the power-supply voltage. If the instrument is used without switching the voltage setting, not only the instrument will break down, but the user may also be exposed to danger; accordingly, contact the Sakura Finetek Technical Support representative or local representative when the instrument is installed to switch the voltage setting according to the voltage on the facility side. The factory setting is 230 V.

Checking the Accessories

Product models in the instruction sheet are as shown below. Pay attention that accessories are different depending on the model.

Model

Overview

Tissue-Tek PrismaPlus (Model Prisma-P-AD)

w/ Drying Function

Tissue-Tek PrismaPlus (Model Prisma-P-AS)

w/ Drying/Heating/Special Staining Functions

Tissue-Tek PrismaPlus (Model Prisma-P-ED)

w/ Drying Function

Tissue-Tek PrismaPlus (Model Prisma-P-ES)

w/ Drying/Heating/Special Staining Functions

Tissue-Tek PrismaPlus (Model DRS-Prisma-P-JD)

w/ Drying Function

Tissue-Tek PrismaPlus (Model DRS-Prisma-P-JS)

w/ Drying/Heating/Special Staining Functions

Tissue-Tek PrismaPlus (Model DRS-Prisma-P-JCS)

w/ Drying/Heating/Special Staining Functions

Confirm that all accessories (refer to the table below) are present and that the instrument and

accessories are free from damage, and if the instrument is damaged or any of the accessories is short or damaged, contact the Sakura Finetek sales representative.

No.

Name of part

Q'ty

Remarks

3-position reservoir tray (2-phase stainless) *3

-AD, -AS, -JD, -JS, -JCS

3-position reservoir tray

-ED. -ES

4-position reservoir tray (2-phase stainless) *3

-AD, -AS, -JD, -JS, -JCS

4-position reservoir tray

-ED. -ES

Control panel protection sheet

Comes attached to the control panel

Paraffin tray

2 7

Hot air outlet panel, drying station

Bushing (bolt cap)

Heating solution reservoir

-AS, -ES, -JS, -JCS

Water supply hose (faucet connection type)

-ED, -ES, -JD, -JS, -JCS

Nipple (1/2 inch)

1(2)

2 for -ED, -ES

Bushing (1/2 x 3/4 inch)

-ED, -ES, -JD, -JS, -JCS

Bushing (machined)

-AD, -AS

Drain hose unit (38 mm × 2 mm + cuffs)

Drain elbow

1 16

Spiral wire band

1 17

Hose clamp

Power cord 100/115 VAC power plug

Not applicable to -JCS

Power cord 230 VAC power plug

1 20

Ferrite core

For input in case of power outage

Activated carbon filter

2 22

20-slide basket

10 23

20-slide basket adapter

10 24

Nozzle

Of the four pieces, two are preinstalled on the instrument.

Wash cap

Of the four pieces, two are preinstalled on the instrument.

Cap for covering water supply port

Of the four pieces, two are preinstalled on the instrument.

Cap for LAN port

For LAN port; installed on the port.

Cap for RS232C port

For RS232C port; installed on the port.

CF card

1 30

Station labels (Start, End)

3 types/set

Basket adapter label

1 32

Operating Manual

1 33

Package insert for medical equipment

-JD, -JS

Excerpt from Unpacking/Installation Manual

1 35

Warranty registration card

-JD, -JS

10-slide basket

-AS, -ES, -JS, -JCS

10-slide basket adapter

-AS, -ES, -JS, -JCS

10-slide basket load adapter

-AS, -ES, -JS, -JCS

Special stain solution reservoir

-AS, -ES, -JS, -JCS

Lid, special stain solution reservoir

-AS, -ES, -JS, -JCS

Special stain reservoir tray (2-phase stainless)

-AS, -JS, -JCS

Special stain reservoir tray

-ES

Standard solution reservoir

-AD, -AS, -ED, -ES, -JCS

Solution reservoir with handle

-AD, -AS, -ED, -ES, -JCS

Wash reservoir

-AD, -AS, -ED, -ES, -JCS

Lid, standard solution reservoir

-AD, -AS, -ED, -ES, -JCS

Large lid, 3-position reservoir tray (2-phase stainless)

-AD, -AS, -JCS

Large lid, 3-position reservoir tray

-ED. -ES

Large lid, 4-position reservoir tray (2-phase stainless)

-AD, -AS, -JCS

Large lid, 4-position reservoir tray

-ED. -ES

51 Standard solution reservoir set (sold as a set) *2

-JD, -JS (Breakdown)

Standard solution reservoir

Solution reservoir with handle

Wash reservoir

Lid, standard solution reservoir

Small solution reservoir set (sold as a set) *2

-JD, -JS (Breakdown)

Solution reservoir with handle

Wash reservoir

Lid, standard solution reservoir

Small solution reservoir

Lid, small solution reservoir

20-slide basket load/unload adapter

Strainer 1 -ED. -ES

Street elbow (1/2 inch)

Installation Method

1-8

*1 The accessories are compatible with all models, unless the applicable models are specified in the Remarks field. *2 Select either the standard solution reservoir set or small solution reservoir set. (-JD/-JS only) *3 Housed in the instrument.

Installation Method

1-9

①

②

Checking the Installation (Securing the Instrument)

1. Confirm that the location where the instrument will be installed is flat and sufficiently strong.

2. Two or more persons work together to lift the instrument and move it to the installation location. If the instrument rattles or is inclined too much when installed, adjust the level adjusters underneath the instrument. Also when the instrument is linked to the Tissue-Tek Film or Tissue-Tek Glass g2, use the level adjusters underneath the instrument to adjust the height and inclination of the instrument.

3. Once the instrument has been installed, remove the transport handles and install the attached bushing (bolt caps) in the threaded parts on the system.

Installing the parts around the instrument

● Attach the water supply hose connect it to a water supply facility (tap water supply) meeting the

following conditions:

Pressure: Dynamic pressure = 0.098 to 0.441 MPa

Maximum static pressure = 0.74 MPa Temperature: 30C max. (non-freezing) Faucet: A faucet of general shape having a tip of 12 mm to 17 mm in size. (The

water supply hose cannot be connected to a faucet of any other size or to a

special shaped faucet such as chemical faucet or aerator faucet.) Facility requirements: Use a facility meeting the following items in order to prevent health damage

caused by flow-back:

[1] Water supply facility not directly connected to public water pipes [2] Water supply facility not directly connected to a water supply system

used for drinking water, etc.

[3] Water supply facility designed to prevent flow-back caused by reverse-

siphoning

• Water supply facility equipped with a flow-back protection mechanism meeting the law of each country/region

1. Connect a nipple o the water supply port on the instrument via a bushing, and then screw the threaded part of the water supply hose into the bushing.

2. Pull the flange on the other end of the water supply hose toward the hose and separate the water tap joint.

3. Loosen the four screws on the water tap joint.

4. Tighten the four screws uniformly while pressing the black rubber in the water tap joint against the tip of the water faucet.

5. Turn and tighten the nipple.

6. Insert the water supply hose into the water tap joint with the flange pulled back.

7. Gently pull the water supply hose by hand to confirm that it does not come off from the water tap joint.

]

Threaded part of the water hose

How to separate the water tap joint

[1]

[2]

Water tap joint

Flange on the water supply hose

Nipple Water supply port

Bushing

Nipple

Installation Method

1-10

Spiral wire band

Drain hose

Drain port

Drain elbow

Hose clamp

Drain hose

● Attaching the water drain hose

Facility requirements: [1] Sink, drain ditch or other water drain facility not subject to back pressure

[2] Water drain facility that will not negatively affect the environment or

human body even when chemicals used for staining enter the system

1. Install a spiral wire band on one end of the water drain hose, connect a drain elbow to the drain hose, and then tighten the screws.

2. Install a hose clamp to the drain port on the instrument, and then insert the drain elbow into the drain port.

3. Tighten the screws on the hose clamp using a flathead screwdriver.

4. Connect the other end of the drain hose to a sink having a minimum drain capacity of 15 L/min. (Connect the hose securely so that it will not separate from the sink.)

5. Ensure that the drain port on the instrument is positioned higher than the sink, that the drain hose has no slack in the middle, and that the outlet of the drain hose is away from the water level of the sink by at least 100 mm.

Do not extend the drain hose using a different hose. The drain elbow can be oriented to either right or

left. The drain hose contains vinyl chloride, so be careful not to spill organic solvents onto the hose. After connecting the water supply hose and drain hose, confirm by referring to P. 1-42 that water is not leaking.

● Installation of exhaust system

The instrument generates volatile solution gases, so install it where a ventilation system, local exhaust system or other exhaust facility, or exhaust port leading to the outside of the building, is available. Also, estimate the exhaust gas concentration from the reference value of maximum volume of gas released and the volumetric size of the installation location, and confirm that the ventilation rate of the installed ventilation facility is sufficient to meet the requirements pertaining to the concentrations of substances in the work environment. Ideally the instrument is installed directly near a ventilation facility, if it must be installed away from a ventilation facility; however, use a 38 or 75 exhaust duct adapter (optional) to connect the duct hose to the exhaust port of the instrument, and discharge the exhaust fume near the exhaust facility. Even when no exhaust facility is available, use a duct hose to discharge the exhaust fume to the outside of the building.

Maximum volumes of gases released per unit time (reference value)

• Ethanol: 3.9×10-3 (m3/hr)

• Xylene: 4.8×10-4 (m3/hr)

 Facility Check

• Ventilation system

• Local exhaust system

• Exhaust port

 Required Options

• Exhaust duct adapter, 38 mm dia.

• Exhaust duct adapter, 75 mm dia.

 How to Connect

1. Attach the 38 or 75 exhaust duct adapter to the exhaust port located at the back of the system on the left side.

2. Insert a duct hose into the adapter and tighten it with a wire clamp, etc.

3. Bring the open end of the duct hose to near a ventilation system or local exhaust system or to the outside of the building. The duct hose should not be longer than 10 m, and confirm that the hose is not bent or crushed in the middle. If the ambient temperature is low, drain water may collect inside the duct hose; accordingly, make sure drain water will not collect in any part of the duct hose and also install a drain pan at the tip of the duct hose or take other appropriate measure.

+ 102 hidden pages

Sakura Tissue-Tek Prisma Plus Operating Manual

Specifications and Main Features

Frequently Asked Questions

User Manual