0002783-01 Rev.B
© 2010 Sakura Finetek USA, Inc.
All Rights Reserved
Printed in U.S.A.
Manufactured for:
Sakura Finetek USA, Inc., Torrance, CA 90501 U.S.A.
Sakura Finetek Japan Co., Ltd., Tokyo, 135-0007, Japan
Sakura Finetek Europe B.V., 2804 AV Alphen aan den Rijn, NL
Made in Japan
TABLE OF CONTENTS
Section Page
1 INTRODUCTION
Safety Precautions ................................................................................................................ 1.1
General Description ............................................................................................................... 1.2
Physical Characteristics ........................................................................................................ 1.3
Specifications ........................................................................................................................ 1.7
2 INSTALLATION
General Information .............................................................................................................. 2.1
Environmental Factors........................................................................................................... 2.1
Unpacking ............................................................................................................................. 2.1
Installation of Accessories and Consumable Supplies .......................................................... 2.3
3 CUSTOMIZATION OF SETTINGS
General Information .............................................................................................................. 3.1
Explanation of the Main Menu ............................................................................................... 3.1
4 OPERATING INSTRUCTIONS
Operating Instructions........................................................................................................... 4.1
Routine Operations ............................................................................................................... 4.5
Start of Operation ............................................................................................................. 4.5
Operations Permitted during Operation ............................................................................ 4.9
End of Coverslipping ........................................................................................................ 4.11
End of Operation .............................................................................................................. 4.12
What to do before Prolonged Period of Non-operation .................................................... 4.12
Actions to Be Taken upon Power Outage during Operation .............................................. 4.13
Table of Coverslipped Conditions and Remedial Actions ................................................. 4.14
How to Operate the g2 when Linked to the Prisma .......................................................... 4.17
5 CARE OF INSTRUMENT
General Maintenance ............................................................................................................. 5.1
Inspection Methods and Cleaning .................................................................................... 5.1
Revised 3/30/2010 i
TABLE OF CONTENTS
Section Page
6 TROUBLESHOOTING
General Information .............................................................................................................. 6.1
Troubleshooting Table........................................................................................................... 6.2
Actions to take upon Generation of Errors, Cautions, and Warnings .................................... 6.3
A APPENDIX A GLOSSARY
Glossary ................................................................................................................................ A.1
B APPENDIX B WARNINGS AND CAUTIONS
Warnings ............................................................................................................................... B.1
Cautions ................................................................................................................................ B.2
C APPENDIX C NOTES ON USE
Notes on Use ......................................................................................................................... C.1
ii Revised 3/30/2010
INTRODUCTION
Section 1
Safety Precautions
Operation of the Tissue-Tek® Glas™g2 Automated
Glass Coverslipper requires expert knowledge of the
target application as well as method of use. To use the
system correctly and safely, designate a “Lead System
Operator.”
When the system is delivered, the Lead System
Operator should receive explanation on the handling of
the system directly from the sales representative.
• Read this “Safety Precautions” section
beforehand to ensure the correct use of the
system.
• The cautionary instructions provided herein are
intended to ensure that the system will be used
safely in a manner preventing the operator from
injury and property damage. These instructions
provide important safety information that must be
heeded at all times.
• Images shown in the manual may partially differ
from those of the actual product.
• In this manual, instructions pertaining to different
levels of potential hazards are classified as
warnings, cautions, and notes and are indicated
under “Warning,” “Caution”, and “Note,”
respectively. Each class of instructions is defined
below.
NOTES, CAUTIONS, WARNINGS, and other safety
related labeling are provided throughout this manual
to indicate levels of potential hazards as defined
below:
NOTE Indicates a reminder or other helpful
information.
The symbols used on the labels attached to the
system are explained below. Labels bearing one of the
following symbols provide particularly important
information to know in order to ensure safety of the
operator, improve work efficiency, and protect the system
from damage. Be sure to check these labels and
understand the specified instructions before commencing
any work.
HOT SURFACE Indicates hot surfaces. Take precautions
to prevent burns.
BIOHAZARD Possibility of infections depending on
the type of specimens processed.
Prevent infections by using Personal
Protective Equipment (PPE) as required
by OSHA and any applicable state or
local regulations.
A label bearing this symbol specifies a
cautionary item. Improper handling
against the instruction may put the
operator in danger or damage the
system. Always follow the instructions.
A label bearing this symbol specifies an
action that must be taken. Always
follows the instructions.
A label bearing this symbol specifies a
prohibited item. Always follow the
instructions.
CAUTION Indicates a potential hazard in which
failure to follow instruction may result in
damage to the Tissue-Tek
and/or other property, or may give poor
processing results.
WARNING Identifies a potential hazard in which
failure to follow instructions may result in
serious injury to the operator and/or
other personnel.
Revised 3/30/2010 1.1
®
Glas™ g2
INTRODUCTION
General Description
The automatic coverslipper Tissue-Tek® Glas™ g2
(Figure 1-A) is a system that dispenses mounting medium
onto stained histopathological and cytopathological
samples on glass slides and coverslips the samples using
cover glasses.
• Up to 240 slides (corresponding to 12 baskets) can
be coverslipped in a continuous manner.
• The dispense volume of mounting medium and
coverslipping speed can be adjusted in real time.
• Different dispense volumes of mounting medium and
coverslipping speeds can be programmed according
to different tissue thicknesses.
• For best results, it is recommended to use the
Tissue-Tek
6419 (Bottle size is 500 mL).
• Drying fans are provided to reduce the drying time
after coverslipping.
• Activated carbon filters can be used to minimize
hazardous fumes.
• Using an optional link system, link the g2 with an
automatic slide strainer (Tissue-Tek
achieve automation of all tissue operations from
staining to coverslipping.
®
Glas™ Mounting Medium, product code
®
Prisma®), to
1.2 Revised 3/30/2010
Figure 1-A
Section 1
Physical Characteristics
Figure 1-B
Figure 1-C
Front Side (Figure 1-B)
Cover .1. — Open this cover to fill cover glass or remove
an empty basket.
Loading station access door .2. —The loading station in
which baskets are placed is found behind this door.
Unloading door .3. —Receiving racks are placed behind
this door.
Power switch .4. —This switch is used to turn the system
power on/off.
Control panel .5. — This control panel is used to operate
the system.
Back Side (Figure 1-C)
Exhaust outlet .6. —Air from the interior of the system is
exhausted through this outlet. An external exhaust duct of
a diameter of 38 mm or 75 mm in size can be connected.
Circuit protector .7. —This is a safety component used
to protect against power surge.
Power inlet .8. —Connect the power cable here.
Contact terminal .9. —When the system is linked to an
automated stainer, these terminals connect to the link
cable.
Serial number label .10. — The serial number of the
system is specified on this label.
Revised 3/30/2010 1.3
INTRODUCTION
[14]
[16]
[17] [11] [10]
Instrument Interior (Figure 1-D)
[15]
[12]
[13]
Figure 1-D
Mounting medium bottle area .11. —A 500 mL bottle
containing mounting medium must be placed in this area.
For best results, use the Tissue-Tek
®
Glas™ Mounting
Medium, product code 6419.
Loading station .12. —A xylene station in which baskets
are placed. The loading station can accommodate up to
three baskets.
Loading station receiver .13. —The base that facilitates
smooth removal and insertion of the loading station.
Unloading area .14. —Receiving racks are placed in this
area. Up to 12 receiving racks can be placed.
Receiving rack .15.—Slides that have been coverslipped
are automatically placed in each receiving rack.
Carousel (empty basket carousel) .16. — After coverslipping, empty baskets are placed here. The carousel can
accommodate up to 12 baskets.
Fume control (activated carbon filter drawer) .17. —
Activated carbon filters that adsorb the fumes generated
from the system are installed here.
1.4 Revised 3/30/2010
Section 1
[25] [26]
[27]
[31] [30] [29] [28]
[27]
[21
[20
[19
[18
Instrument Interior (Figure 1-E)
[24]
Figure 1-E
Station 1 (slide retrieval) .18. — The slide retrieval arm
takes out slides from the baskets and places them in this
station. The slides that have been taken out from the
baskets are moved to station 2.
Station 2 (dispensing) .19. — At this station, mounting
medium is dispensed onto slides through the dispensing
nozzle. After mounting medium has been dispensed onto
the slides, the slides are moved to station 3.
Station 3 (coverslipping) .20. — At this station, cover
glasses are picked up and carefully placed onto slides.
The coverslipped slides are moved to station 4.
Station 4 (storage) .21. — At this station, coverslipped
slides are placed in receiving racks.
Drying fan 1 .22. — This fan removes excess xylene
after coverslipping to promote the drying of mounting
medium.
Drying fan 2 .23. — This fan removes xylene attached to
the back of the slide to promote the drying of the back of
the slide.
Cover glass holder .24. — Cover glasses are placed in
this holder.
Cover glass arm .25. — This arm picks up and retrieves
cover glasses one at a time and places them onto slides.
Dispensing nozzle down lever .26. — This lever is used
to lower the dispensing nozzle and dip it in the anti-drying
bottle.
Slide retrieval arm .27. — This arm grabs each slide and
retrieves it from a basket.
Dispensing nozzle .28. — This nozzle dispenses
mounting medium. The dispensing nozzle moves linearly
to dispense mounting medium onto a slide along a straight
line.
Waste container .29. — This container is used to remove
the excess mounting medium from the tip of the
dispensing nozzle.
Anti-drying bottle .30. — The tip of the dispensing
nozzle sits in this bottle when the instrument is idle. Put
solvent for mounting medium (xylene is recommended) in
this bottle to prevent the tip of the nozzle from clogging.
Dispensing pump reservoir .31. — Put solvent for
mounting medium (xylene is recommended) here to
prevent mounting medium from sticking to the interior of
the pump.
Revised 3/30/2010 1.5
INTRODUCTION
[2] [1]
[3]
[4]
[9]
[8]
[14]
[5]
Control Panel (Figure 1-F)
[6]
[7]
[10]
[12]
[13]
[15]
Figure 1-F
[11
POWER switch .1. — This switch is used to turn the
power on/off.
POWER lamp .2. — This lamp remains on while the
power is on.
SPEED setting knob .3. — A rotary switch for changing
the coverslipping speed. The coverslipping speed can be
changed to one of eight levels from 1 (lowest speed) to 8
(highest speed).
VOLUME setting knob .4. — A rotary switch for changing
the amount of mounting medium dispensed. The volume
of mounting medium can be changed to one of 10 levels
from 30 µL to 120 µL.
Display screen .5. — The main screen that shows the
current system status and other information required for
operation.
SIZE key .6. — The size of cover glass can be changed
using this key. The four sizes of cover glass are 40, 50,
55, or 60 mm.
PRIME key .7. — Press this key once, and mounting
medium will be dispensed by the specified volume. Press
and hold the key for 3 seconds or more, and mounting
medium will be dispensed 10 times consecutively.
STOP key .8. — Use this key to pause the coverslipping
operation or cancel the initial priming of mounting medium.
START key .9. — Use this key to start a new coverslipping operation or resume the paused coverslipping
operation.
RACK lamp .10. — This lamp illuminates when the
unloading door is opened to place or remove receiving
racks.
LOAD lamp .11. — This lamp illuminates when the
loading station access door is opened to place baskets.
EXIT key .12. — Use this key to cancel the current setting
operation without saving the changes and return to the
previous screen.
MENU key .13. — Pressing this key displays the menu
screen. Press the key twice, and the sub menu screen
will appear.
ENTER key .14. — Press this key to accept and save the
current setting.
</> keys .15. — Use these keys to select a desired item
from a list. Pressing the < key moves the cursor up or to
the left, while pressing the > key moves the cursor down
or to the right.
1.6 Revised 3/30/2010
Section 1
Specifications
Models
Model Name/Description
Tissue-Tek
6500
(115 VAC) (USA)
Tissue-Tek
6501
(100 VAC) (Asia)
Tissue-Tek® Glas™ g2 Automated Glass Coverslipper
6502
(230 VAC) (Europe)
Power Supply Requirements
Model Power Frequency Amps
6500 115 VAC ± 10% @ <15A 50/60 Hz 2.2A
6501
100 VAC + 10%/−5% @ <15A
6502
Electrical Safety
For Model 6500 –
cETL Certification
• Safety requirements UL61010A-1:2004,
• CAN/CSA-C22.2 No.61010-1:2004
For Model 6501 –
CE Marking
• Safety requirements IEC61010-1:2001
• EMC Directive IEC61326-1:2002
• In-vitro Diagnostic Directive IEC61010-2-101:2002
• Risk management ISO14971:2000
For Model 6502 –
Amendment of Pharmaceutical Affairs Law
• Safety requirements J IS C1010-1:2005
• EMC JIS C1806-1:2001
• Risk management JIS T14971:2003
Dimensions
750 (W) x 620 (D) x 750 (H) mm
®
Glas™ g2 Automated Glass Coverslipper
®
Glas™ g2 Automated Glass Coverslipper
230 VAC ± 10% @ < 7A
50/60 Hz
50/60 Hz
2.5A
1.2A
Instrument Weight
Approximately 110 kg (242.5 lbs)
Operating Conditions
Operational Temperature: 10°C to 40°C (50°F to 104°F)
Relative Humidity: 30-85% (non-condensing)
Protect from direct sunlight
Capacity
Coverslipping Speed: up to 420 slides/hour
Acceptable Dimensions for Slides
Dimensions:
25.0 to 26.0 (width) x 75.0 to 76.0 (length) mm
Thickness:
0.9 to 1.2 mm
Acceptable Dimensions for Cover Glass
Dimensions:
24 (width) x 40, 50, 55, 60 (length) mm
25 (width) x 40, 50, 55, 60 (length) mm
If 25-mm wide cover glasses are used, the system may
have to be readjusted.
Thickness:
0.9 to 1.2 mm
Compatible Solvent
Xylene
D-Limonene-based substitutes
Aliphatic-Hydrocarbon-based substitutes
Compatible Mounting Medium
Xylene-based mounting medium
D-Limonene-based mounting medium
Aliphatic-Hydrocarbon-based mounting medium
Revised 3/30/2010 1.7
INTRODUCTION
1.8 Revised 3/30/2010
INSTALLATION & SETUP
Section 2
General Information
This section provides detailed installation and setup
instructions for the Tissue-Tek
Coverslipper. The installation steps must be followed
correctly to ensure proper operation and service. Read this
Operating Manual carefully before attempting to operate
the instrument. Follow all instructions carefully.
The Tissue-Tek Glas g2 Coverslipper is a precision
instrument and must be handled accordingly. Rough
handling or dropping the instrument will disturb or damage
internal components. Always handle the instrument with
care.
Select a place where sufficient clearance can be provided around the instrument. A clearance of at least 10 cm
is required for the top and left side of the instrument. To
the right of the instrument, at least 30 cm should be
provided.
®
TM
Glas
g2 Automated Glass
Environmental Factors
Unpacking
Before unpacking the Glas g2 coverslipper, please
refer to the detailed instructions in the separate document
titled: “Tissue-Tek
call the local Sakura sales representative for assistance.
CAUTION: The instrument is very heavy and large;
therefore, it is strongly recommended that it always
be lifted and transported by at least two people, one
positioned on each side.
®
Glas™ g2 Unpacking Procedures” or
As with all sensitive electronic instruments, prolonged
exposure to excessive humidity and temperature should
be avoided. Temperature and humidity should be held
relatively constant. The ambient temperature range for
operating the instrument is 10°C to 40°C (50°F to 104°F).
The ambient operating humidity range is 30-85% relative
humidity.
Locate the instrument in a well-ventilated area,
avoiding exposure to corrosive vapors and extreme
variations in temperature or humidity. The area should be
clean and dust-free and have a firm, level surface capable
of holding at least 110 kg (242.5 lbs.) of weight. Be sure it
is near a power source that meets the electrical
requirements specified on the rating label located on the
rear of this instrument. The power receptacle must be
grounded and should be a dedicated line. Avoid proximity
to direct sunlight, open windows, sinks, ovens, hot plates,
open burners, or radiators.
Revised 3/30/2010 2.1
INSTALLATION & SETUP
Unpacking the Accessories (Figure 2-A)
When opening the accessory box, confirm that all
accessories have been included with the instrument:
a 20-Slide basket adapter (10), product code 6138
a 20-Slide baskets (10), product code 4768
eceiving racks (12), product code 6504
a R
ower cord (1), product code A40-105-11
a P
a L
oading station (1), product code N94-013-00
a B
asket receiver, loading station (1), product code
N94-014-00
a L
id, loading station (1), product code N94-015-00
a Anti-drying bottle (1), product code 6423
aste container (1), product code 6430
a W
ispensing tray (1), product code N94-041-00
a D
a M
ounting medium bottle tray (1), product code
N94-357-00
a C
over glass tray (1), product code N94-041-00
a S
lide protect seat (1), product code N94-373-00
a Priming bottle (1), product code 6433
aste bottle (1), product code 6505
a W
ire brush (1), product code D8-60-0000
a W
a L
evel (1), product code D9-60-0000
a A
ctivated carbon cartridge (1), product code 6160
®
a Tissue-Tek
code 6168
a Operating manual (1), product code 0002783-01
Options (Figure 2-B)
22 Cover glass holder (250-slide type)
24 x 40 mm (1 piece), product code N94-084-00
24 x 50 mm (1 piece), product code N94-085-00
24 x 55 mm (1 set), product code N94-086-00
24 x 60 mm (1 set), product code N94-087-00
23 Exhaust hose set 1, product code 6506
24 Exhaust hose set 2, product code 6507
Glas™g2 link system (1 set), product
Shipped separately:
• Tissue-Tek
code 6419
®
Glas™ Mounting Medium, product
Figure 2-A
Figure 2-B
2.2 Revised 3/30/2010
Section 2
Installation of Accessories and
Consumable Supplies
Installation of Accessories
This section explains how to install the various
accessories.
Using the Basket Adapter (Figures 2-C &
2-D)
1. Align the direction of the “UP SIDE” mark on the top
face of the basket adapter with the direction of the
“UP SIDE” mark on the side face of the basket.
2. Insert one tip of the basket adapter into a basket
adapter hole.
3. While gently pulling the basket adapter wire, insert
the other tip into a basket adapter hole.
NOTE: Place the slides in the basket by making sure
their tissue slide aligns with the “UP SIDE” direction.
Figure 2-C
“UP SIDE” mark
Figure 2-D
Installing the Receiving Rack (Figure 2-E)
1. Open the unloading door.
2. Grab the receiving rack (Figure 2-F) handle in such a
way that the slide unloading area faces to the right
side, and then place the receiving rack in the
recessed area of the unloading area (indicated by the
shaded box in Figure 2-G). Up to 12 receiving racks
can be placed.
3. Close the unloading door.
Front side of
receiving rack
Figure 2-E
Back side of
receiving rack
Figure 2-F
Figure 2-G
“UP SIDE” mark
Revised 3/30/2010 2.3
Recessed area where receiving racks are placed
INSTALLATION & SETUP
Hole in basket receiver
Installing the Loading Station
1. Align the direction of the basket receiver with the
2. Fill with solvent until the specified level (approx. 1.5
3. Open the loading station access door, grab the
4. Place the loading station on the loading station
5
. Place the dedicated lid on the loading station and
6. Close the loading station access door.
direction of the loading station. Insert the pin inside
the loading station into the hole in the basket receiver,
then insert the loading station all the way to the end
(Figure 2-H & Figure 2-I).
Basket receiver
Specified fluid level
Loading station pin
Loading station
Label side
Figure 2-H
Figure 2-I
View of Complete Assembly
liters) is reached.
handle, and pull out the loading station receiver.
receiver by making sure the basket receiver faces the
right side (Figure 2-J).
push the loading station back into the system (Figure
2-K).
Handle
Loading station
receiver
Label side
Figure 2-J
Figure 2-K
Lid
CAUTION: If the amount of solvent is insufficient, air
bubbles may be introduced and tissues may dry up.
2.4 Revised 3/30/2010
Section 2
Fill solvent until this
til this line just
Figure 2-N
Connecting the Power Cable
1. Turn the power switch to OFF.
2. Connect the power cable to the power inlet on the
system (Figure 2-L).
3. Connect the power plug to a power outlet with a
grounding terminal (3P).
Installing the Mounting Medium Bottle
Tray
1. Place the mounting medium bottle tray (A) by aligning it
with the pins provided on the inner face of the bottom
plate of the mounting medium storage area (Figure 2-M).
2. Place the mounting medium bottle tray (B) by aligning
it with the pins provided on the outer face of the
bottom of the mounting medium storage area (Figure
2-N).
Mounting medium
bottle tray (A)
Power inlet
Figure 2-L
Figure 2-M
Mounting medium
bottle tray (B)
Installing the Dispensing Tray
1. Turn the power switch to OFF.
2. Open the cover.
3. Place the dispensing tray in the system.
4. Fill solvent in the anti-drying bottle until just below the
lid (Figure 2-O). The dispensing nozzle should sit
above the fluid level so the solvent vapors prevent the
mounting medium at the tip of the dispensing nozzle
from solidifying. Be careful
dispensing nozzle contact the solvent.
Figure 2-O
CAUTION: Do not use toluene or any liquid containing
toluene. Instrument malfunction or failure may result.
Check the amount of solvent for mounting medium,
such as xylene, before use. Failure to do so may
cause the tip of the dispensing nozzle to stick,
resulting in incorrect coverslipping operation and
negative consequences on the tissues.
not to let the tip of the
Fill solvent un
below the lid.
Revised 3/30/2010 2.5
INSTALLATION & SETUP
Dispensing
Waste
Anti-drying bottle
5. Place the anti-drying bottle and waste container in the
dispensing tray (Figure 2-P). First, push back the
dispensing nozzle in and then place the bottle
(Figures 2-Q, 2-R, & 2-S).
tray
container
Figure 2-P
Dispensing nozzle
Dispensing tray
Figure 2-Q
Figure 2-R
Figure 2-S
Waste Container Anti-drying
bottle
6. After the anti-drying bottle and waste container have
been placed, lift the dispensing nozzle and return the
nozzle so that its tip enters the hole in the anti-drying
bottle (Figure 2-T).
Dispensing nozzle
Anti-drying bottle
Figure 2-T
2.6 Revised 3/30/2010
Section 2
Installation of Accessories and
Consumable Supplies
This section explains how to install the consumable
supplies.
Cover Glass Tray
1. Turn the power switch to OFF.
2. Open the cover.
3. Position the cover glass tray by aligning it with the
two pins provided on the side toward the front of the
instrument (Figure 2-U & 2-V).
NOTE: In certain situations such as when two cover
glasses are picked up together, the cover glass tray
prevents the cover glass or glasses that have dropped
during the retrieval operation from further dropping into the
system.
Cover glass tray
Figure 2-U
Figure 2-V
Slide Glass Sheet
1. Turn the power switch to OFF.
2. Open the cover.
3. Move the cover glass holder to the right.
4. Lift the receiving rack arm.
5. Place the sheet in the tray below the receiving rack
arm (Figure 2-W).
Slide glass sheet
Figure 2-W
Link System
By opening the aperture provided in the right side
face of the system (Figure 2-X), it is possible to link the
system with the automatic stainer (Tissue-Tek
Prisma
™). For details on installing the link system,
consult the Sakura dealer.
Aperture
Figure 2-X
®
Revised 3/30/2010 2.7
INSTALLATION & SETUP
Exhaust Hose
By opening an adapter to connect an exhaust hose is
provided at the back of the system. A hose with a
diameter of 38 or 75 mm can be connected.
Options
• Exhaust hose set 1 (38 mm type): 38 mm hose (5
m), hose bands, cuffs (Figure 2-Y)
• Exhaust hose set 2 (75 mm type): 75 mm hose (5
m), hose bands, cuffs (Figure 2-Z)
Exhaust hose set 1
Exhaust hose set 2
Figure 2-Y
Figure 2-Z
Installing the Activated Carbon Filters
1. Press on the front of the fume control door to release
the lock, and then pull out the fume control door
(Figure 2-AA).
2. Place two new activated carbon cartridges in the
activated carbon cartridge storage area. If used
activated carbon cartridges are present, remove both
cartridges and replace them with new ones.
3. Push back the fume control unit door into the system
until it clicks into place.
Exhaust
part
Activated carbon cartridge and
storage area
Figure 2-AA
2.8 Revised 3/30/2010
Section 2
sensor
Installing the Mounting Medium Bottle
The mounting medium suction tube is equipped with a
level detection sensor. Set the tube and sensor correctly.
1. Open the lid of the mounting medium bottle. Use a
500-mL bottle containing mounting medium, such as
the Tissue-Te k
code 6419.
2. Tilt the mounting medium introduction tube toward the
front of the instrument and lift the tube. (Figure 2-BB).
3. Slowly insert the mounting medium introduction tube
and level detection sensor into the mounting medium
bottle (Figure 2-BB).
CAUTION: If the mounting medium bottle is inserted
too quickly, air bubbles may enter the mounting
medium. If the system is operated continuously with
air bubbles in the mounting medium, air bubbles may
be coverslipped together with the tissues.
4. Return the mounting medium introduction tube and
level detection sensor to the vertical position, and
then lower them to the bottom. If a gap generates
between the disk-shaped cap and the bottle, adjust
the gap along the mounting medium bottle
CAUTION: When installing mounting medium for the
first time or change the type of mounting medium,
“initial priming of mounting medium” must be
performed. For the procedure to perform this
operation, refer to Section 3, “Initial Priming”.
Mounting medium
introduction tube
and level detection
Mounting
medium bottle
®
Glas™ Mounting Medium, product
Figure 2-BB
Adjusting the Gap along the Mounting
Medium Bottle
1. Loosen the two screws at the cap installation
mechanism.
2. Gently push the cap against the mounting medium
bottle and while the cap is in contact with the bottle,
tighten the two screws that were loosened (Figure 2CC).
Screws
Cause the disk-shaped cap to
make a full contact so that no
gap remains here, and then
tighten the screws to secure the
cap in position.
Figure 2-CC
Revised 3/30/2010 2.9
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