Sakura Tissue-Tek Glas g2 Operating Manual

Page 1

Page 2

0002783-01 Rev.B

Printed in U.S.A.

Manufactured for: Sakura Finetek USA, Inc., Torrance, CA 90501 U.S.A. Sakura Finetek Japan Co., Ltd., Tokyo, 135-0007, Japan Sakura Finetek Europe B.V., 2804 AV Alphen aan den Rijn, NL

Made in Japan

Page 3

TABLE OF CONTENTS

Section Page

1 INTRODUCTION

Safety Precautions ................................................................................................................ 1.1

General Description ............................................................................................................... 1.2

Physical Characteristics ........................................................................................................ 1.3

Specifications ........................................................................................................................ 1.7

2 INSTALLATION

General Information .............................................................................................................. 2.1

Environmental Factors........................................................................................................... 2.1

Unpacking ............................................................................................................................. 2.1

Installation of Accessories and Consumable Supplies .......................................................... 2.3

3 CUSTOMIZATION OF SETTINGS

General Information .............................................................................................................. 3.1

Explanation of the Main Menu ............................................................................................... 3.1

4 OPERATING INSTRUCTIONS

Operating Instructions........................................................................................................... 4.1

Routine Operations ............................................................................................................... 4.5

Start of Operation ............................................................................................................. 4.5

Operations Permitted during Operation ............................................................................ 4.9

End of Coverslipping ........................................................................................................ 4.11

End of Operation .............................................................................................................. 4.12

What to do before Prolonged Period of Non-operation .................................................... 4.12

Actions to Be Taken upon Power Outage during Operation .............................................. 4.13

Table of Coverslipped Conditions and Remedial Actions ................................................. 4.14

How to Operate the g2 when Linked to the Prisma .......................................................... 4.17

5 CARE OF INSTRUMENT

General Maintenance ............................................................................................................. 5.1

Inspection Methods and Cleaning .................................................................................... 5.1

Revised 3/30/2010 i

Page 4

TABLE OF CONTENTS

Section Page 6 TROUBLESHOOTING

General Information .............................................................................................................. 6.1

Troubleshooting Table........................................................................................................... 6.2

Actions to take upon Generation of Errors, Cautions, and Warnings .................................... 6.3

A APPENDIX A GLOSSARY

Glossary ................................................................................................................................ A.1

B APPENDIX B WARNINGS AND CAUTIONS

Warnings ............................................................................................................................... B.1

Cautions ................................................................................................................................ B.2

C APPENDIX C NOTES ON USE

Notes on Use ......................................................................................................................... C.1

ii Revised 3/30/2010

Page 5

INTRODUCTION

Section 1

Safety Precautions

Operation of the Tissue-Tek® Glas™g2 Automated Glass Coverslipper requires expert knowledge of the target application as well as method of use. To use the system correctly and safely, designate a “Lead System Operator.”

When the system is delivered, the Lead System Operator should receive explanation on the handling of the system directly from the sales representative.

• Read this “Safety Precautions” section

beforehand to ensure the correct use of the system.

• The cautionary instructions provided herein are

intended to ensure that the system will be used safely in a manner preventing the operator from injury and property damage. These instructions provide important safety information that must be heeded at all times.

• Images shown in the manual may partially differ

from those of the actual product.

• In this manual, instructions pertaining to different

levels of potential hazards are classified as warnings, cautions, and notes and are indicated under “Warning,” “Caution”, and “Note,” respectively. Each class of instructions is defined below.

NOTES, CAUTIONS, WARNINGS, and other safety related labeling are provided throughout this manual to indicate levels of potential hazards as defined below:

NOTE Indicates a reminder or other helpful

information.

The symbols used on the labels attached to the system are explained below. Labels bearing one of the following symbols provide particularly important information to know in order to ensure safety of the operator, improve work efficiency, and protect the system from damage. Be sure to check these labels and understand the specified instructions before commencing any work.

HOT SURFACE Indicates hot surfaces. Take precautions

to prevent burns.

BIOHAZARD Possibility of infections depending on

the type of specimens processed. Prevent infections by using Personal Protective Equipment (PPE) as required by OSHA and any applicable state or local regulations.

A label bearing this symbol specifies a cautionary item. Improper handling against the instruction may put the operator in danger or damage the system. Always follow the instructions.

A label bearing this symbol specifies an action that must be taken. Always follows the instructions.

A label bearing this symbol specifies a prohibited item. Always follow the instructions.

CAUTION Indicates a potential hazard in which

failure to follow instruction may result in damage to the Tissue-Tek and/or other property, or may give poor processing results.

WARNING Identifies a potential hazard in which

failure to follow instructions may result in serious injury to the operator and/or other personnel.

Revised 3/30/2010 1.1

Glas™ g2

Page 6

INTRODUCTION

General Description

The automatic coverslipper Tissue-Tek® Glas™ g2 (Figure 1-A) is a system that dispenses mounting medium onto stained histopathological and cytopathological samples on glass slides and coverslips the samples using cover glasses.

• Up to 240 slides (corresponding to 12 baskets) can

be coverslipped in a continuous manner.

• The dispense volume of mounting medium and

coverslipping speed can be adjusted in real time.

• Different dispense volumes of mounting medium and

coverslipping speeds can be programmed according

to different tissue thicknesses.

• For best results, it is recommended to use the

Tissue-Tek

6419 (Bottle size is 500 mL).

• Drying fans are provided to reduce the drying time

after coverslipping.

• Activated carbon filters can be used to minimize

hazardous fumes.

• Using an optional link system, link the g2 with an

automatic slide strainer (Tissue-Tek

achieve automation of all tissue operations from

staining to coverslipping.

Glas™ Mounting Medium, product code

Prisma®), to

1.2 Revised 3/30/2010

Figure 1-A

Page 7

Section 1

Physical Characteristics

Figure 1-B

Figure 1-C

Front Side (Figure 1-B)

Cover .1. — Open this cover to fill cover glass or remove

an empty basket.

Loading station access door .2. —The loading station in which baskets are placed is found behind this door.

Unloading door .3. —Receiving racks are placed behind this door.

Power switch .4. —This switch is used to turn the system power on/off.

Control panel .5. — This control panel is used to operate the system.

Back Side (Figure 1-C)

Exhaust outlet .6. —Air from the interior of the system is exhausted through this outlet. An external exhaust duct of a diameter of 38 mm or 75 mm in size can be connected.

Circuit protector .7. —This is a safety component used to protect against power surge.

Power inlet .8. —Connect the power cable here.

Contact terminal .9. —When the system is linked to an

automated stainer, these terminals connect to the link cable.

Serial number label .10. — The serial number of the system is specified on this label.

Revised 3/30/2010 1.3

Page 8

INTRODUCTION

[14]

[16]

[17] [11] [10]

Instrument Interior (Figure 1-D)

[15]

[12]

[13]

Figure 1-D

Mounting medium bottle area .11. —A 500 mL bottle

containing mounting medium must be placed in this area. For best results, use the Tissue-Tek

Glas™ Mounting

Medium, product code 6419.

Loading station .12. —A xylene station in which baskets are placed. The loading station can accommodate up to three baskets.

Loading station receiver .13. —The base that facilitates smooth removal and insertion of the loading station.

Unloading area .14. —Receiving racks are placed in this area. Up to 12 receiving racks can be placed.

Receiving rack .15.—Slides that have been coverslipped are automatically placed in each receiving rack.

Carousel (empty basket carousel) .16. — After coverslipping, empty baskets are placed here. The carousel can accommodate up to 12 baskets.

Fume control (activated carbon filter drawer) .17. — Activated carbon filters that adsorb the fumes generated from the system are installed here.

1.4 Revised 3/30/2010

Page 9

Section 1

[25] [26]

[27]

[31] [30] [29] [28]

[27] [21

[20

[19

[18

Instrument Interior (Figure 1-E)

[24]

Figure 1-E

Station 1 (slide retrieval) .18. — The slide retrieval arm

takes out slides from the baskets and places them in this station. The slides that have been taken out from the baskets are moved to station 2.

Station 2 (dispensing) .19. — At this station, mounting medium is dispensed onto slides through the dispensing nozzle. After mounting medium has been dispensed onto the slides, the slides are moved to station 3.

Station 3 (coverslipping) .20. — At this station, cover glasses are picked up and carefully placed onto slides. The coverslipped slides are moved to station 4.

Station 4 (storage) .21. — At this station, coverslipped slides are placed in receiving racks.

Drying fan 1 .22. — This fan removes excess xylene after coverslipping to promote the drying of mounting medium.

Drying fan 2 .23. — This fan removes xylene attached to the back of the slide to promote the drying of the back of the slide.

Cover glass holder .24. — Cover glasses are placed in this holder.

Cover glass arm .25. — This arm picks up and retrieves cover glasses one at a time and places them onto slides.

Dispensing nozzle down lever .26. — This lever is used to lower the dispensing nozzle and dip it in the anti-drying bottle.

Slide retrieval arm .27. — This arm grabs each slide and retrieves it from a basket.

Dispensing nozzle .28. — This nozzle dispenses mounting medium. The dispensing nozzle moves linearly to dispense mounting medium onto a slide along a straight line.

Waste container .29. — This container is used to remove the excess mounting medium from the tip of the dispensing nozzle.

Anti-drying bottle .30. — The tip of the dispensing nozzle sits in this bottle when the instrument is idle. Put solvent for mounting medium (xylene is recommended) in this bottle to prevent the tip of the nozzle from clogging.

Dispensing pump reservoir .31. — Put solvent for mounting medium (xylene is recommended) here to prevent mounting medium from sticking to the interior of the pump.

Revised 3/30/2010 1.5

Page 10

INTRODUCTION

[2] [1]

[3]

[4]

[9]

[8]

[14]

[5]

Control Panel (Figure 1-F)

[6]

[7]

[10]

[12]

[13]

[15]

Figure 1-F

[11

POWER switch .1. — This switch is used to turn the power on/off.

POWER lamp .2. — This lamp remains on while the power is on.

SPEED setting knob .3. — A rotary switch for changing the coverslipping speed. The coverslipping speed can be changed to one of eight levels from 1 (lowest speed) to 8 (highest speed).

VOLUME setting knob .4. — A rotary switch for changing the amount of mounting medium dispensed. The volume of mounting medium can be changed to one of 10 levels from 30 µL to 120 µL.

Display screen .5. — The main screen that shows the current system status and other information required for operation.

SIZE key .6. — The size of cover glass can be changed using this key. The four sizes of cover glass are 40, 50, 55, or 60 mm.

PRIME key .7. — Press this key once, and mounting medium will be dispensed by the specified volume. Press and hold the key for 3 seconds or more, and mounting medium will be dispensed 10 times consecutively.

STOP key .8. — Use this key to pause the coverslipping operation or cancel the initial priming of mounting medium.

START key .9. — Use this key to start a new coverslipping operation or resume the paused coverslipping operation.

RACK lamp .10. — This lamp illuminates when the unloading door is opened to place or remove receiving racks.

LOAD lamp .11. — This lamp illuminates when the loading station access door is opened to place baskets.

EXIT key .12. — Use this key to cancel the current setting operation without saving the changes and return to the previous screen.

MENU key .13. — Pressing this key displays the menu screen. Press the key twice, and the sub menu screen will appear.

ENTER key .14. — Press this key to accept and save the current setting.

</> keys .15. — Use these keys to select a desired item from a list. Pressing the < key moves the cursor up or to the left, while pressing the > key moves the cursor down or to the right.

1.6 Revised 3/30/2010

Page 11

Section 1

Specifications

Models

Model Name/Description

Tissue-Tek

6500

(115 VAC) (USA)

Tissue-Tek

6501

(100 VAC) (Asia)

Tissue-Tek® Glas™ g2 Automated Glass Coverslipper

6502

(230 VAC) (Europe)

Power Supply Requirements

Model Power Frequency Amps

6500 115 VAC ± 10% @ <15A 50/60 Hz 2.2A

6501

100 VAC + 10%/−5% @ <15A

6502

Electrical Safety

For Model 6500 –

cETL Certification

• Safety requirements UL61010A-1:2004,

• CAN/CSA-C22.2 No.61010-1:2004

For Model 6501 –

CE Marking

• Safety requirements IEC61010-1:2001

• EMC Directive IEC61326-1:2002

• In-vitro Diagnostic Directive IEC61010-2-101:2002

• Risk management ISO14971:2000

For Model 6502 –

Amendment of Pharmaceutical Affairs Law

• Safety requirements J IS C1010-1:2005

• EMC JIS C1806-1:2001

• Risk management JIS T14971:2003

Dimensions

750 (W) x 620 (D) x 750 (H) mm

Glas™ g2 Automated Glass Coverslipper

230 VAC ± 10% @ < 7A

50/60 Hz

2.5A

1.2A

Instrument Weight

Approximately 110 kg (242.5 lbs)

Operating Conditions

Operational Temperature: 10°C to 40°C (50°F to 104°F) Relative Humidity: 30-85% (non-condensing) Protect from direct sunlight

Capacity

Coverslipping Speed: up to 420 slides/hour

Acceptable Dimensions for Slides

Dimensions:

25.0 to 26.0 (width) x 75.0 to 76.0 (length) mm

Thickness:

0.9 to 1.2 mm

Acceptable Dimensions for Cover Glass

Dimensions: 24 (width) x 40, 50, 55, 60 (length) mm 25 (width) x 40, 50, 55, 60 (length) mm If 25-mm wide cover glasses are used, the system may have to be readjusted.

Thickness:

0.9 to 1.2 mm

Compatible Solvent

Xylene D-Limonene-based substitutes Aliphatic-Hydrocarbon-based substitutes

Compatible Mounting Medium

Xylene-based mounting medium D-Limonene-based mounting medium Aliphatic-Hydrocarbon-based mounting medium

Revised 3/30/2010 1.7

Page 12

INTRODUCTION

1.8 Revised 3/30/2010

Page 13

INSTALLATION & SETUP

Section 2

General Information

This section provides detailed installation and setup instructions for the Tissue-Tek Coverslipper. The installation steps must be followed correctly to ensure proper operation and service. Read this Operating Manual carefully before attempting to operate the instrument. Follow all instructions carefully.

The Tissue-Tek Glas g2 Coverslipper is a precision instrument and must be handled accordingly. Rough handling or dropping the instrument will disturb or damage internal components. Always handle the instrument with care.

Select a place where sufficient clearance can be provided around the instrument. A clearance of at least 10 cm is required for the top and left side of the instrument. To the right of the instrument, at least 30 cm should be provided.

Glas

g2 Automated Glass

Environmental Factors

Unpacking

Before unpacking the Glas g2 coverslipper, please refer to the detailed instructions in the separate document titled: “Tissue-Tek call the local Sakura sales representative for assistance.

CAUTION: The instrument is very heavy and large; therefore, it is strongly recommended that it always be lifted and transported by at least two people, one positioned on each side.

Glas™ g2 Unpacking Procedures” or

As with all sensitive electronic instruments, prolonged exposure to excessive humidity and temperature should be avoided. Temperature and humidity should be held relatively constant. The ambient temperature range for operating the instrument is 10°C to 40°C (50°F to 104°F). The ambient operating humidity range is 30-85% relative humidity.

Locate the instrument in a well-ventilated area, avoiding exposure to corrosive vapors and extreme variations in temperature or humidity. The area should be clean and dust-free and have a firm, level surface capable of holding at least 110 kg (242.5 lbs.) of weight. Be sure it is near a power source that meets the electrical requirements specified on the rating label located on the rear of this instrument. The power receptacle must be grounded and should be a dedicated line. Avoid proximity to direct sunlight, open windows, sinks, ovens, hot plates, open burners, or radiators.

Revised 3/30/2010 2.1

Page 14

INSTALLATION & SETUP

Unpacking the Accessories (Figure 2-A)

When opening the accessory box, confirm that all

accessories have been included with the instrument:

a 20-Slide basket adapter (10), product code 6138

a 20-Slide baskets (10), product code 4768

eceiving racks (12), product code 6504

a R

ower cord (1), product code A40-105-11

a P

a L

oading station (1), product code N94-013-00

a B

asket receiver, loading station (1), product code

N94-014-00

a L

id, loading station (1), product code N94-015-00

a Anti-drying bottle (1), product code 6423

aste container (1), product code 6430

a W

ispensing tray (1), product code N94-041-00

a D

a M

ounting medium bottle tray (1), product code

N94-357-00

a C

over glass tray (1), product code N94-041-00

a S

lide protect seat (1), product code N94-373-00

a Priming bottle (1), product code 6433

aste bottle (1), product code 6505

a W

ire brush (1), product code D8-60-0000

a W

a L

evel (1), product code D9-60-0000

a A

ctivated carbon cartridge (1), product code 6160

a Tissue-Tek

code 6168

a Operating manual (1), product code 0002783-01

Options (Figure 2-B)

22 Cover glass holder (250-slide type)

24 x 40 mm (1 piece), product code N94-084-00 24 x 50 mm (1 piece), product code N94-085-00 24 x 55 mm (1 set), product code N94-086-00 24 x 60 mm (1 set), product code N94-087-00

23 Exhaust hose set 1, product code 6506

24 Exhaust hose set 2, product code 6507

Glas™g2 link system (1 set), product

Shipped separately:

• Tissue-Tek

code 6419

Glas™ Mounting Medium, product

Figure 2-A

Figure 2-B

2.2 Revised 3/30/2010

Page 15

Section 2

Installation of Accessories and Consumable Supplies

Installation of Accessories

This section explains how to install the various accessories.

Using the Basket Adapter (Figures 2-C & 2-D)

1. Align the direction of the “UP SIDE” mark on the top face of the basket adapter with the direction of the “UP SIDE” mark on the side face of the basket.

2. Insert one tip of the basket adapter into a basket adapter hole.

3. While gently pulling the basket adapter wire, insert the other tip into a basket adapter hole.

NOTE: Place the slides in the basket by making sure their tissue slide aligns with the “UP SIDE” direction.

Figure 2-C

“UP SIDE” mark

Figure 2-D

Installing the Receiving Rack (Figure 2-E)

1. Open the unloading door.

2. Grab the receiving rack (Figure 2-F) handle in such a way that the slide unloading area faces to the right side, and then place the receiving rack in the recessed area of the unloading area (indicated by the shaded box in Figure 2-G). Up to 12 receiving racks can be placed.

3. Close the unloading door.

Front side of receiving rack

Figure 2-E

Back side of receiving rack

Figure 2-F

Figure 2-G

“UP SIDE” mark

Revised 3/30/2010 2.3

Recessed area where receiving racks are placed

Page 16

INSTALLATION & SETUP

Hole in basket receiver

Installing the Loading Station

1. Align the direction of the basket receiver with the

2. Fill with solvent until the specified level (approx. 1.5

3. Open the loading station access door, grab the

4. Place the loading station on the loading station

. Place the dedicated lid on the loading station and

6. Close the loading station access door.

direction of the loading station. Insert the pin inside the loading station into the hole in the basket receiver, then insert the loading station all the way to the end (Figure 2-H & Figure 2-I).

Basket receiver

Specified fluid level

Loading station pin

Loading station

Label side

Figure 2-H

Figure 2-I

View of Complete Assembly

liters) is reached.

handle, and pull out the loading station receiver.

receiver by making sure the basket receiver faces the right side (Figure 2-J).

push the loading station back into the system (Figure 2-K).

Handle

Loading station

receiver

Label side

Figure 2-J

Figure 2-K

Lid

CAUTION: If the amount of solvent is insufficient, air bubbles may be introduced and tissues may dry up.

2.4 Revised 3/30/2010

Page 17

Section 2

Fill solvent until this

til this line just

Figure 2-N

Connecting the Power Cable

1. Turn the power switch to OFF.

2. Connect the power cable to the power inlet on the system (Figure 2-L).

3. Connect the power plug to a power outlet with a grounding terminal (3P).

Installing the Mounting Medium Bottle Tray

1. Place the mounting medium bottle tray (A) by aligning it with the pins provided on the inner face of the bottom plate of the mounting medium storage area (Figure 2-M).

2. Place the mounting medium bottle tray (B) by aligning it with the pins provided on the outer face of the bottom of the mounting medium storage area (Figure 2-N).

Mounting medium

bottle tray (A)

Power inlet

Figure 2-L

Figure 2-M

Mounting medium

bottle tray (B)

Installing the Dispensing Tray

1. Turn the power switch to OFF.

2. Open the cover.

3. Place the dispensing tray in the system.

4. Fill solvent in the anti-drying bottle until just below the lid (Figure 2-O). The dispensing nozzle should sit above the fluid level so the solvent vapors prevent the mounting medium at the tip of the dispensing nozzle from solidifying. Be careful dispensing nozzle contact the solvent.

Figure 2-O

CAUTION: Do not use toluene or any liquid containing toluene. Instrument malfunction or failure may result. Check the amount of solvent for mounting medium, such as xylene, before use. Failure to do so may cause the tip of the dispensing nozzle to stick, resulting in incorrect coverslipping operation and negative consequences on the tissues.

not to let the tip of the

Fill solvent un below the lid.

Revised 3/30/2010 2.5

Page 18

INSTALLATION & SETUP

Dispensing

Waste Anti-drying bottle

5. Place the anti-drying bottle and waste container in the dispensing tray (Figure 2-P). First, push back the dispensing nozzle in and then place the bottle (Figures 2-Q, 2-R, & 2-S).

tray

container

Figure 2-P

Dispensing nozzle

Dispensing tray

Figure 2-Q

Figure 2-R

Figure 2-S

Waste Container Anti-drying

bottle

6. After the anti-drying bottle and waste container have been placed, lift the dispensing nozzle and return the nozzle so that its tip enters the hole in the anti-drying bottle (Figure 2-T).

Dispensing nozzle

Anti-drying bottle

Figure 2-T

2.6 Revised 3/30/2010

Page 19

Section 2

Installation of Accessories and Consumable Supplies

This section explains how to install the consumable supplies.

Cover Glass Tray

1. Turn the power switch to OFF.

2. Open the cover.

3. Position the cover glass tray by aligning it with the two pins provided on the side toward the front of the instrument (Figure 2-U & 2-V).

NOTE: In certain situations such as when two cover glasses are picked up together, the cover glass tray prevents the cover glass or glasses that have dropped during the retrieval operation from further dropping into the system.

Cover glass tray

Figure 2-U

Figure 2-V

Slide Glass Sheet

1. Turn the power switch to OFF.

2. Open the cover.

3. Move the cover glass holder to the right.

4. Lift the receiving rack arm.

5. Place the sheet in the tray below the receiving rack arm (Figure 2-W).

Slide glass sheet

Figure 2-W

Link System

By opening the aperture provided in the right side face of the system (Figure 2-X), it is possible to link the system with the automatic stainer (Tissue-Tek Prisma

™). For details on installing the link system,

consult the Sakura dealer.

Aperture

Figure 2-X

Revised 3/30/2010 2.7

Page 20

INSTALLATION & SETUP

Exhaust Hose

By opening an adapter to connect an exhaust hose is provided at the back of the system. A hose with a diameter of 38 or 75 mm can be connected.

Options

• Exhaust hose set 1 (38 mm type): 38 mm hose (5

m), hose bands, cuffs (Figure 2-Y)

• Exhaust hose set 2 (75 mm type): 75 mm hose (5

m), hose bands, cuffs (Figure 2-Z)

Exhaust hose set 1

Exhaust hose set 2

Figure 2-Y

Figure 2-Z

Installing the Activated Carbon Filters

1. Press on the front of the fume control door to release the lock, and then pull out the fume control door (Figure 2-AA).

2. Place two new activated carbon cartridges in the activated carbon cartridge storage area. If used activated carbon cartridges are present, remove both cartridges and replace them with new ones.

3. Push back the fume control unit door into the system until it clicks into place.

Exhaust part

Activated carbon cartridge and storage area

Figure 2-AA

2.8 Revised 3/30/2010

Page 21

Section 2

sensor

Installing the Mounting Medium Bottle

The mounting medium suction tube is equipped with a level detection sensor. Set the tube and sensor correctly.

1. Open the lid of the mounting medium bottle. Use a 500-mL bottle containing mounting medium, such as the Tissue-Te k code 6419.

2. Tilt the mounting medium introduction tube toward the front of the instrument and lift the tube. (Figure 2-BB).

3. Slowly insert the mounting medium introduction tube and level detection sensor into the mounting medium bottle (Figure 2-BB).

CAUTION: If the mounting medium bottle is inserted too quickly, air bubbles may enter the mounting medium. If the system is operated continuously with air bubbles in the mounting medium, air bubbles may be coverslipped together with the tissues.

4. Return the mounting medium introduction tube and level detection sensor to the vertical position, and then lower them to the bottom. If a gap generates between the disk-shaped cap and the bottle, adjust the gap along the mounting medium bottle

CAUTION: When installing mounting medium for the first time or change the type of mounting medium, “initial priming of mounting medium” must be performed. For the procedure to perform this operation, refer to Section 3, “Initial Priming”.

Mounting medium introduction tube and level detection

Mounting medium bottle

Glas™ Mounting Medium, product

Figure 2-BB

Adjusting the Gap along the Mounting Medium Bottle

1. Loosen the two screws at the cap installation mechanism.

2. Gently push the cap against the mounting medium bottle and while the cap is in contact with the bottle, tighten the two screws that were loosened (Figure 2CC).

Screws

Cause the disk-shaped cap to make a full contact so that no gap remains here, and then tighten the screws to secure the cap in position.

Figure 2-CC

Revised 3/30/2010 2.9

Page 22

INSTALLATION & SETUP

Cover

Cover glass

Holder hole

Placing the Cover Glasses

NOTE: Place commercial holders provided by cover glass

manufacturers directly in the system. Dedicated cover glass holders are also provided as options (sold separately). When using dedicated cover glass holders, place cover glasses in the holders (Figure 2-DD).

1. Open the instrument cover.

2. Place the cover glass holder so that the tab of the cover glass holder fits in the holder.

3. Close the instrument cover.

The following eight types of cover glasses can be used:

24 x 40, 24 x 50, 24 x 55, 24 x 60 (mm)

25 x 40, 25 x 50, 25 x 55, 25 x 60 (mm)

glass

holder

Figure 2-DD

Tab Holder hole

2.10 Revised 3/30/2010

Page 23

CUSTOMIZATION OF SETTINGS

<ME N U > 1 - 1 0 1 . COU NT

2. E XTE NDED PRI ME

3. E RROR LOG

Section 3

General Information

This section explains the operations of the main menu

screen and how to change each setting.

Explanation of the Main Menu

The menu screen allows selection of various menu items during the initial installation or checking/changing the system settings (Figure 3-A).

Pressing the MENU key will bring the menu screen. Press the < and > keys to highlight the desired item, and then press the ENTER key to select.

Menu Item Descriptions

1. COUNT- Indicates the number of slides that have

been coverslipped. The counter can be reset. See page 3.2.

2. EXTENDED PRIME- Mounting medium is dispensed

continuously from the tip of the nozzle. Select this menu item when loading mounting medium for the first time, changing the mounting medium, or when air bubbles have entered the tubing. See page 3.3.

3. ERROR LOG- Provides a log of errors that have

occurred. See page 3.4.

Figure 3-A

4. PROGRAM EDIT- Sets the names and details of

coverslipping programs that specify coverslipping conditions. Up to nine coverslipping programs can be stored in memory, each specifying a different cover glass size, coverslipping speed, and volume of mounting medium. See page 3.4.

5. PROGRAM LOCK- Prevents program changes

during coverslipping. Select “Lock” or “Unlock.” Realtime change of coverslipping speed and volume of mounting medium is enabled only when “Unlock” is selected. See page 3.5.

6. KEY SOUND- Selects whether or not to output a

sound when a key is pressed. See page 3.5.

7. END SOUND- Changes the sound indicating the

completion of coverslipping. Select a desired sound from three pattern choices. See page 3.5.

8. ALARM- Selects a desired sound, from four pattern

choices, to indicate an error condition. See page 3.6.

9. START METHOD- Selects from two start methods:

The Start Key method required pressing the start key to initiate coverslipping while the Unloading Station Access Door method will initiate coverslipping as soon as that door is closed. See page 3.6.

10. CLEANING- Cleans the interior of the mounting

medium tubing using xylene or flushes out the mounting medium left in the tube. The operation is the same as what is performed when “EXTENDED PRIME” is selected. Under “CLEANING,” however, reagent flow is at a higher speed. Do not perform this operation for the purpose of initial priming of mounting medium, because air bubbles will form. See page

3.7.

11. SOFTWARE VERSION- Displays the software

version. See page 3.7.

Revised 3/30/2010 3.1

Page 24

CUSTOMIZATION OF SETTINGS

<COUNT> COUNT:012345

[STOP] to reset

<COUNT RESET> Are you sure?

[ENTER] to reset

<ME N U > 1 - 1 0 1 . COU NT

2. E XTE NDED PRI ME

3. E RROR LOG

Viewing/Changing the Count

How to view/change the number of slides that have been coverslipped is explained (Figure 3-B).

NOTE: This menu item is available only when the system is on standby or paused, or when the system check screen is displayed.

1. Press the MENU key.

2. Press the < and > keys to highlight 1, “COUNT,” and then press the ENTER key and the counter screen appears (Figure 3-C).

3. Press the STOP ke y. The COUNT clear confirmation screen appears (Figure 3-D).

Figure 3-B

Figure 3-D: Counter Reset Confirmation Screen

The count is indicated as a 6-digit number and when the count reaches “999999,” it will return to “1.” The count increments every time a slide is coverslipped.

To manually increase or decrease the count press the

< or > key.

Figure 3-C: Counter Screen

3.2 Revised 3/30/2010

Page 25

Section 3

<EXTENDED PRI ME> [ ENTER] t o pr i me syst em [ EXI T] t o exi t

<EXTENDED PRI ME> Priming

[ S T OP ] t o st op

Initial Priming

When installing the mounting medium bottle for the first time or when introducing a new type of mounting medium, the dispense tubing must be flushed out. A series of operations performed for these purposes is called “initial priming.”

In initial priming, mounting medium is pulled out continuously from the mounting medium bottle and dispensed continuously from the tip of the dispensing nozzle.

To install mounting medium for the first time or change the type of mounting medium, also refer to “Installing/Replacing the Mounting Medium Bottle.”

NOTE: This menu item is available only when the system is on standby or paused or when the system check screen is displayed.

1. Press the MENU k e y.

2. Press the < and > keys to highlight 2, “EXTENDED

PRIME,” and then press the ENTER key (Figure 3-E).

3. The extended prime screen appears (Figure 3-F)

4. Open the loading station access door.

5. Open the cover and take out the entire dispensing

tray from the system. Remove the entire dispensing

tray and set the waste tray for initial priming at a

position below the dispensing nozzle.

6. Remove the lid of the waste bottle, and while lifting

the dispensing nozzle, place the anti-drying bottle

below the dispensing nozzle (on the two guide rails).

7. Fill the priming bottle with solvent (normally xylene),

then insert the tip of the tube into the introduction tube

(Figure 3-G). Squeeze bottle to introduce solvent into

dispensing channel. Fill channel until solvent drips

out of the dispensing nozzle.

8. When initial prime has been completed, usually all of

the solvent has been removed from the dispensing

channel and mounting medium has been introduced.

If not, run initial prime one more time.

Revised 3/30/2010 3.3

Figure 3-E: Initial Priming Screen

Figure 3-F: Screen during Dispenses

CAUTION: If the system has not been used for an extended period of time, the solvent may not be dispensed evenly when this operation is performed. In this case, the solvent must be forcibly introduced to the priming tubing using a pump bottle for initial priming. For details, contact the Sakura Customer Service Center the Sakura local distributor.

Introduction tube

Figure 3-G

Priming bottle Waste bottle

Page 26

CUSTOMIZATION OF SETTINGS

<ERROR L OG> Cl ear t he er r or l og?

[ ENTER] t o cl ear

01: 99 111111111 02: 100 111112111 03: 111 111120111 04: 200 111150111

Viewing the Error Log

Creating a Program

Up to 99 errors can be displayed, with the newest number representing the latest error.

When more than 99 errors have occurred, the oldest error will be deleted and the latest error will be added.

The displayed items are line number, error number, and count (system slide count).

NOTE: When the power is turned off, error code “99” is automatically registered in the error log.

NOTE: This menu item is available only when the system is on standby or paused or when the system check screen is displayed.

1. Press the MENU k e y.

2. Press the < and > keys to highlight 3, “ERROR LOG,”

and then press the ENTER ke y.

3. The error log screen appears (Figure 3-H).

4. Pressing the STOP key opens the error log clear

confirmation screen (Figure 3-I).

5. Press the ENTER key to clear the error log. To return

to the menu screen without clearing the error log,

press the EXIT key.

Figure 3-H: Error log Screen

Figure 3-I: Error Log Clear Screen

A program consists of three items, namely CG (cover

glass) size, volume of mounting medium, and coverslipping speed. A program name can also be assigned.

NOTE: This menu item is available only when the system is on standby or paused or when the system check screen is displayed.

1. Press the MENU k e y.

2. Press the < and > keys to highlight 4, “@PROGRAM CREATION@,” and then press the ENTER key.

3. The program name setting screen appears (Figure 3-I).

Figure 3-I: Program Name Setting

4. If no name is set, the field is blank. Enter a desired name consisting of up to eight characters.

• <, > Use these keys to change the

program number,

• SPEED This key moves the cursor inside

the name field. This field is surrounded by two brackets.

• VO LUME This key moves the cursor to

highlight the desired characters.

• ENTER Use this key to select and display in

the name field the character highlighted using the VOL UME ke y.

• SIZE Use this key to switch between

upper case and lower case characters.

• EXIT Press this key to return to the menu

screen.

from P1 to P9.

3.4 Revised 3/30/2010

Page 27

Section 3

<P1:HE-1 > SIZE:60mm SPEED:7 VOLUME:30 [ENTER] to save

<END SOUND> 1 / 2 / 3 [<][>] to select [ENTER] to save

<KEY SOUND> OFF / ON [<][>] to select [ENTER] to save

L o c k / UnLock

[<][>] to select [ENTER] to save

5. When the MENU key is pressed once again, the

program edit screen appears (Figure 3-J).

Figure 3-J: Program Edit Screen

6. Set the cover glass size, coverslipping speed and volume of mounting medium for each program.

7. Press the ENTER key to save the new settings.

Program Lock

Selecting the Key Sound

This function allows the key sound to be turned on/off.

NOTE: This menu item is available only when the system is on standby or paused or when the system check screen is displayed.

1. Press the MENU k e y.

2. Press the < and > keys to highlight 6, “KEY SOUND,” and then press the ENTER ke y.

3. The key sound screen appears (Figure 3-L).

4. Press the < and > keys to select OFF or ON.

5. Press the ENTER key to save the selected setting. To return to the menu screen without saving the change, press the EXIT key.

Access to program editing can be locked during coverslipping.

NOTE: This menu item is available only when the system is on standby or paused or when the system check screen is displayed.

1. Press the MENU key.

2. Press the < and > keys to highlight 5, “PROGRAM LOCK,” and then press the ENTER key.

3. The Lock/Unlock screen appears (Figure 3-K).

4. Press the < and > keys to select Lock or Unlock.

5. Press the ENTER key to save the new settings.

NOTE: When Lock is selected, the coverslipping speed and volume of mounting medium can no longer be changed in real time during coverslipping.

Lock

Figure 3-K: Lock/Unlock Selection Screen

Revised 3/30/2010 3.5

Figure 3-L: Key Sound Setting Screen

Selecting the Coverslipping End Sound

To indicate end of coverslipping there is a choice of three different sound patterns.

NOTE: This menu item is available only when the system is on standby or paused or when the system check screen is displayed.

1. Press the MENU k e y.

2. Press the < and > keys to highlight 7, “END SOUND,” and then press the ENTER ke y.

3. The end sound setting screen appears (Figure 3-M).

Figure 3-M: End Sound Setting Screen

Page 28

CUSTOMIZATION OF SETTINGS

<START METHOD> START KEY [<][>] to select [ENTER] to save

<ALARM> 1 / 2 / 3 / 4 [<][>] to select [ENTER] to save

4. Press the < and > keys to select a desired option from

1, 2, and 3.

• Pattern 1 A short “beep” sound is output six

times repeatedly.

• Pattern 2 An intermediate length “beep” sound is output six times repeatedly.

• Pattern 3 A long “beep” sound is output six

times repeatedly.

5. Press the ENTER key to save the selected setting. To

return to the menu screen without saving the change, press the EXIT key.

Selecting the Alarm Volume/Tone

This sets a desired volume and tone for both the alarm sound and the end sound. There are four pattern choices.

NOTE: This menu item is available only when the system is on standby or paused or when the system check screen is displayed.

1. Press the MENU k e y.

2. Press the < and > keys to highlight 8, “ALARM,” and

then press the ENTER ke y.

3. The alarm setting screen appears.

4. Press the < and > keys to select a desired option from

1, 2, 3 and 4 (Figure 3-N).

Pattern Volume Tone

Low pitch

Small

Low pitch

High pitch

Large

High pitch

Figure 3-N

5. Press the ENTER key to save the selected setting. To

return to the menu screen without saving the change,

press the EXIT key (Figure 3-O).

Figure 3-O: Alarm setting Screen

Selecting the Start Method

Select between two start methods using this function.

NOTE: This menu item is available only when the system is on standby or paused or when the system check screen is displayed.

1. Press the MENU k e y.

2. Press the < and > keys to highlight 9, “START METHOD,” and then press the ENTER k e y.

3. The start method setting screen appears (Figure 3-P).

4. Press the < and > keys to select either “START KEY” or “UNLOADING STATION ACCESS DOOR.”

• “START KEY” Operation will start when the

START key is pressed.

• “UNLOADING STATION ACCESS DOOR”

Operation will start when the unloading station access door is closed.

5. Press the ENTER key to save the selected setting. To return to the menu screen without saving the change, press the EXIT key.

Figure 3-P: Start Method Setting Screen

3.6 Revised 3/30/2010

Page 29

Section 3

<CLEANING> Set a container

[START] to start

<CLEANING> In process 10 [STOP] to stop

MAIN:N94-900-00 SUB1:N94-910-00 SUB2:N94-920-00 SUB3:N94-930-00

Cleaning the Interior of the Mounting Medium Tubing

The purpose of this procedure is to clean the interior of the mounting medium tubing using a solvent, preferably xylene. Do not use this cleaning function to prime the mounting medium as the flow rate is too high and will generate air bubbles in the line

Figure 3-R: Cleaning in Progress Screen

CAUTION: In this mode, the dispensing pump is operated at the maximum stroke just like during initial priming. Suction and dispense operations are performed. Also, do not use this mode for initial priming of mounting medium because air bubbles will generate as mounting medium is introduced.

NOTE: This menu item is available only when the system

is on standby or paused or when the system check screen is displayed.

1. Press the MENU k e y.

2. Press the < and > keys to highlight 10, “CLEANING,”

and then press the ENTER ke y.

3. The cleaning screen appears (Figure 3-Q).

4. Place a full bottle containing solvent.

5. Remove the entire dispensing tray from the system

and set the waste tray for initial priming at a position

below the dispensing nozzle.

6. Press the STA R T key to start cleaning.

7. To cancel dispensing of solvent, press the STOP key

(Figure 3-R). Once the dispensing stops, the display

returns to the menu screen.

8. Remove the waste tray for initial priming and set the

original dispensing tray again.

9. To return to the menu screen, press the EXIT key.

Viewing the Software Version

View the software version using this function.

NOTE: This menu item is available only when the system is on standby or paused or when the system check screen is displayed.

1. Press the MENU k e y.

2. Press the < and > keys to highlight 11, “SOFTWARE VERSION,” and then press the ENTER key.

3. The software version screen appears (Figure 3-S).

4. To return to the menu screen, press the EXIT key.

Figure 3-S: Software Version Screen

Figure 3-Q: Cleaning Screen

Revised 3/30/2010 3.7

Page 30

CUSTOMIZATION OF SETTINGS

Explanation of Sub Menu Items

This section explains the operations available from the sub menu screen and how to change each setting (Figure 3-T).

When the MENU key is pressed, the menu screen appears. When the MENU key is pressed again, the display switches to the sub menu screen.

The coverslipping performance of this system changes according to the various conditions such as the thickness of glass slide, whether or not the slide is coated, characteristics of mounting medium, tissue type, and temperature/humidity. So that coverslipping is performed properly, the set values of coverslipping conditions used by the system must be changed according to each situation.

The sub menu provides an adjustment menu for achieving optimal coverslipping when the present condition of coverslipping is not favorable.

Press the MENU key to open then menu screen, and then press the MENU key again to move to the sub menu screen.

Highlight a desired item using the < and > keys, and make the selection using the ENTER key.

Figure 3-T

1. SLOWDOWN POSITION- This function changes the

pressure applied when the cover glass is placed on the slide. Use this menu item mainly when air bubbles are present or mounting medium is seeping out around the slide. See page 3.9.

2. COVER GLASS ANGLE- This function changes the

separation angle when a cover glass is picked up. Use this menu item when more than one cover glass is picked up or when cover glasses present cracks. Select one of three values. If the cover glass brand is changed, the value may need to be changed. See page 3.10.

3. PICK UP SPEED- This function changes the speed at

which the glass slide is picked up. When the glass slide pickup speed is changed, the amount of xylene remaining on the slide at the time of pickup will change. Use this menu item mainly when air bubbles are present or mounting medium is seeping out from the slides. If the solvent type is changed, this value may need to be changed. See page 3.11.

4. DISPENSE POINT- This function changes the

position at which to start dispensing mounting medium onto the glass slide, in a front to back direction. Use this menu item mainly when air bubbles are present on the front side of the slide or mounting medium is seeping out from the sides. See page

3.12.

5. FIRST DISPENSE- This function adjusts the volume

of mounting medium to be dispensed at the dispense point. The default value is 20 µL. Use this menu item mainly when air bubbles are present on the front side of the slide or mounting medium is seeping out from the sides. See page 3.13.

6. DRYING FAN TIMER- This function changes the

receiving racks drying time in the unloading area after completion of coverslipping. End of drying will be signaled by an audible alarm. The default is 2 minutes. See page 3.13.

3.8 Revised 3/30/2010

Page 31

Section 3

<Slowdown pos> 60mm 005 [<][>] to select [ENTER] to save

Setting the Slowdown Position

To change the pressure applied to the cover glass during coverslipping, use this function (Figure 3-U).

NOTE: This menu item is available only when the system is on standby or paused or when the system check screen is displayed.

Quality coverslipping can be achieved by pressing on the cover glass with appropriate force. If air bubbles are present or mounting medium seeps out from the sides, take the following actions:

• If air bubbles are present, raise the slowdown

position (increase the pressure).

• If mounting medium seeps out from the sides,

lower the slowdown position (decrease the pressure).

Figure 3-U

1. Press the MENU key twice.

2. Press the < and > keys to highlight 1, “Slowdown position,” and then press the ENTER k e y. The slowdown position setting screen appears (Figure 3-V).

3. Pressing the < key decreases the value, while pressing the > key increases the value.

4. Press the ENTER key to save the selected setting. To return to the menu screen without saving the change, press the EXIT key.

Figure 3-V: Slowdown Position Setting Screen

Revised 3/30/2010 3.9

Page 32

CUSTOMIZATION OF SETTINGS

60mm:16.4゜

[<][>] to select [ENTER] to save

Setting the Cover Glass Angle

Use this function to select a desired angle at which to

pick up the cover glasses.

NOTE: This menu item is available only when the system is on standby or paused or when the system check screen is displayed.

The coverslipping arm operates at an angle when

picking up cover glasses.

This angle is called the “cover glass angle.”

Each cover glass varies depending on its brand and the storage environment. If the cover glass angle is not appropriate for the specific type of cover glass, cover glasses may crack when picked up or more than one cover glass may be picked up together. When this occurs, take the following actions:

• If two cover glasses are often picked up together,

increase the cover glass angle.

• If cover glasses often crack, reduce the cover

glass angle.

Coverslipping arm

Cover glass

1. Press the MENU key twice.

2. Press the < and > keys to highlight 2, “Cover Glass

Type,” and then press the ENTER key.

3. The cover glass angle setting screen appears (Figure

3-W).

4. Press the SIZE key and select the cover glass size for

which the angle is to be changed.

5. Press the < and > keys to change the cover glass

angle for each size.

6. Press the ENTER key to save the selected setting. To

return to the menu screen without saving the change,

press the EXIT key.

Figure 3-W: Cover Glass Angle Setting Screen

3.10 Revised 3/30/2010

Cover glass angle

Figure 3-X

Page 33

Section 3

<Pick-up speed> 1 / 2 / 3 / 4 / 5 [<][>] to select [ENTER] to save

Setting the Slide Pickup Speed

Use this function to select a desired speed at which to pick up the slide.

NOTE: This menu item is available only when the system is on standby or paused or when the system check screen is displayed.

The pickup speed setting must be changed according to the water repellency of glass slide and viscosity of mounting medium. The amount of solvent remaining on the slide can be controlled by changing the pickup speed (Figure 3-Z).

NOTE: As the pickup speed decreases the amount of solvent remaining on the slide dec

TE: As the viscosity of mounting medium increases

the amount of solvent remaining on the slide increases.

If the amount of solvent on the glass slide is not enough during coverslipping and mounting medium does not spread sufficiently, increase the pickup speed. If the amount of solvent on the glass slide is excessive during coverslipping and mounting medium runs or seeps out around the slide, decrease the pickup speed.

reases.

Figure 3-Y: Slide Pickup Speed Setting Screen

Mounting Medium and Slide Pickup Speed

Applicable mounting medium

Xylene Substitute Speed 1

Low-viscosity mounting medium

(Default)

High-viscosity mounting medium 1

High-viscosity mounting medium 2

Figure 3-Z

Slide Pickup

Speed

Speed 2

Speed 3

Speed 4

Speed 5

NOTE: In general, xylene substitutes have a slower rate of volatilization than xylene and thus tend to cause mounting medium to run or seep out around the slide. Accordingly, when using a xylene substitute, the slide pickup speed usually needs to be reduced.

1. Press the MENU key twice.

2. Press the < and > keys to highlight 3, “Pick up speed,”

and then press the ENTER ke y.

3. The pick up speed setting screen appears (Figure 3-Y).

4. Press the < and > keys to select a desired option from

1, 2, 3, 4 and 5. The smaller the value, the lower the

SG slide pickup speed becomes and the amount of

solvent attaching to the glass slide decreases. The

greater the value, the higher the SG slide pickup

speed becomes and the amount of solvent attaching

to the glass slide increases.

5. Press the ENTER key to save the selected setting. To

return to the menu screen without saving the change,

press the EXIT key.

Revised 3/30/2010 3.11

Page 34

CUSTOMIZATION OF SETTINGS

<Dispense point>60mm

[START] to start

<Dispense point>60mm 005 [<][>] to select [ENTER] to save

First dispense

Setting the Dispense Point

To select the initial position at which to start dispensing mounting medium onto the glass slide, use this function. The setting should be changed under certain conditions such as when air bubbles are present on the front end of the side of the slide or mounting medium seeps out, especially at the front end of the slide (Figure 3-AA).

An optimum point can be set for each size of cover glass.

NOTE: This menu item is available only when the system is on standby or paused or when the system check screen is displayed.

• If air bubbles enter on the front side of the slide,

move the point toward the front end of the slide.

• If mounting medium seeps out on the front side

of the slide, move the point towards the end of the slide.

1. Press the MENU key twice.

2. Press the < and > keys to highlight 4, “Dispense

point,” and then press the ENTER key.

3. The dispense start point setting screen appears

(Figure 3-BB).

4. Pressing the < key moves the dispense point towards

the front end of the slide, while pressing the > key

moves the dispense point towards the back end of the

slide.

5. Press the ENTER key to save the selected setting

(Figure 3-CC). To return to the menu screen without saving the change, press the EXIT ke y.

Figure 3-BB: Dispense Point Setting Screen

Figure 3-CC: Dispense Point Selection Screen

Figure 3-AA

3.12 Revised 3/30/2010

Page 35

Section 3

<First Dispense> Offset:0 [<][>] to select [ENTER] to save

<Drying Fan Timer> 2min. 30sec. [<][>] to select [ENTER] to save

Setting the First Dispense Volume

During coverslipping, mounting medium is dispensed twice. For the first dispense action, the nozzle remains stationary and mounting medium is dispensed at the current position. For the second dispense action, mounting medium is dispensed as the nozzle moves (Figure 3-DD).

This can change the first dispensing volume of mounting medium using this first dispense volume setting.

NOTE: This menu item is available only when the system is on standby or paused or when the system check screen is displayed.

• If air bubbles enter on the front side (counter-frost

side), increase the first dispense volume.

• If mounting medium drips at a forward position,

decrease the first dispense volume.

1. Press the MENU key twice.

2. Press the < and > keys to highlight 5, “First dispense,”

and then press the ENTER ke y.

3. The first dispense volume setting screen appears

(Figure 3-EE).

4. Pressing the < key decreases the value, while

pressing the > key increases the value.

5. Press the ENTER key to save the selected setting. To

return to the menu screen without saving the change,

press the EXIT key.

First dispense action

Dispensed along a line

Figure 3-DD

Figure 3-EE: First Dispense Volume Setting Screen

Setting the Slide Drying Time

It is possible to program the amount of time slides dry

in the unloading area.

So that the cover glass pasting positions will not shift when the receiving racks containing coverslipped slides are removed, a desired wait time can be set in 30-second increments over a range of 0 to 10 minutes to dry the slides in the unloading area. When drying is completed, a buzzer will sound to notify the completion of drying.

NOTE: This menu item is available only when the system is on standby or paused or when the system check screen is displayed.

1. Press the MENU key twice.

2. Press the < and > keys to highlight 6, “Drying Fan

Timer,” and then press the ENTER key.

3. The drying fan timer setting screen appears (Figure 3-

FF).

4. Pressing the < key decreases the value, while

pressing the > key increases the value.

5. Press the ENTER key to save the selected setting. To

return to the menu screen without saving the change,

press the EXIT key.

Figure 3-FF: Slide Drying Fan Timer Setting Screen

Revised 3/30/2010 3.13

Page 36

CUSTOMIZATION OF SETTINGS

3.14 Revised 3/30/2010

Page 37

OPERATING INSTRUCTIONS

Start of Operation

Operations permitted

for details.)

If coverslipping is to

to the standby screen End of Operation

details.

Start of Operation See page 4.5.

End of Operation See page 4.11.

Power Off See page 4.11.

Section 4

Operating Instructions

This section explains the sequence of operating procedures. The basic flow of operation is shown in the chart below.

Flow of Coverslipping Operation Chart

Pre-operational Checks See page 4.1.

• Turn on the power.

• Remove the lid of the loading station.

• Check the solvent volume.

• Cleaning spilled mounting medium.

• Place receiving racks in the unloading area.

• Confirm that no basket is left in the carousel.

• Set cover glass holders and cover glasses.

• Check the volume of mounting medium.

• Check the cover glass size, coverslipping speed,

and volume of mounting medium.

• Select a coverslipping program.

• Check if the CG size shown on the screen

matches the size of the CG holder actually set.

• Pull out the loading station and set baskets. Close the loading station access door and start coverslipping.

during coverslipping (Refer to page 4.9

• Remove the receiving racks.

• Remove the empty baskets.

• Turn off the power and place the lid on the

loading station.

Refer to P. 36 for

be continued, return

and add baskets.

Refer to P. 42 for

Pre-Operational Checks

This section explains the required steps before

operation.

Turning On the Power

1. Press the power switch on the control panel to turn on the power. If the power does not turn on, check if the power cable is connected to the power outlet.

2. Once the power has been turned on, the initial screen is displayed for approximately 5 seconds and then switches to the system check screen. The following prompts are displayed on the screen:

• Are there sufficient cover glasses? If not, add

cover glasses.

• Is the volume of mounting medium sufficient? If

not, change the mounting medium bottle.

• Are there any slides remaining on transfer arm?

3. Add/change respective items as necessary. When all

4. When Enter is pressed, the home positioning screen

5. When the home positioning is completed, the display

Remove the remaining slides, if any.

items have been reviewed, press the ENTER key to initialize instrument into the home position.

appears. Pressing stop during home positioning procedure will stop the homing process. However, the instrument needs to be in home position to start coverslipping.

switches to standby mode.

Revised 3/30/2010 4.1

Page 38

OPERATING INSTRUCTIONS

Checking the Solvent Volume

If the solvent volume in the loading station, anti-drying bottle, or dispensing pump reservoir is at or below the specified level, add solvent.

 Loading station (Figure 4-A)

The loading station can accommodate approximately 1.5 L of solvent (use of xylene is recommended). The upper and lower levels are marked inside the loading station, so add solvent to a level between these marks.

NOTE: To prevent contamination, filter or change the solvent in the loading station daily.

 Anti-drying bottle (Figure 4-B)

Add solvent to the lower limit mark just below the lid just covering the bottle.

Figure 4-A

CAUTION: If solvent runs out, the mounting medium at the tip of the dispensing nozzle may solidify and cause closing. If the mounting medium at the tip of the dispensing nozzle solidifies, coverslipping may not be performed and the tissues may sustain significant damage. Solidified mounting medium at the tip of the dispensing nozzle can also cause the system to fail. Accordingly, confirm that solvent has been added to the specified position.

 Dispensing pump reservoir (Figure 4-C)

Remove the lid and add solvent for mounting medium to the shoulder of the dispensing pump reservoir.

CAUTION: If solvent runs out, the pump that feeds the mounting medium may clog up and become defective. If the pump clogs up, coverslipping may not be performed and the tissues may sustain significant damage. Accordingly, confirm that solvent has been added to the specified position.

Figure 4-B

Figure 4-C

4.2 Revised 3/30/2010

Page 39

Section 4

Cleaning Spilled Mounting Medium

If mounting medium remains attached to the waste containers and receiving racks, coverslipping may be affected. Before coverslipping is started, clean the waste containers and receiving racks or replace them with clean ones.

CAUTION: If mounting medium remains attached to the waste container (spatula part) or receiving rack, the subsequent coverslipping process may be affected.

Placing Empty Receiving Racks in the Unloading Area

Up to 12 receiving racks can be placed in the unloading area (Figure 4-D).

1. Hold each receiving rack so that the slide unloading

area faces the right side (Figure 4-E).

2. A step is provided on the front side of the unloading

area, and there is a recessed area in which each rack

fits. Place the receiving rack in this recessed area

(Figure 4-F). Take note that if the rack is set on the

front side of the recessed area or above the step, the

system sensor will not detect the rack and this

position will not be used.

CAUTION: If the receiving racks placed in the unloading area have no empty slots, the process will stop. Be sure to place empty receiving racks.

Front side of receiving rack

Figure 4-D

Back side of

Recessed area where receiving racks are placed

receiving rack

Figure 4-E

Figure 4-F

Revised 3/30/2010 4.3

Page 40

OPERATING INSTRUCTIONS

Removal of Baskets in the Carousel

Checking the Volume of Mounting Medium

After all glass slides have moved to the coverslipping station, the empty baskets will be stored in the carousel. If the carousel has no empty slots, coverslipping will not start. Remove all baskets.

Checking the Cover Glass Size

Check the displayed information of the cover glass holder and cover glass box used for coverslipping.

The cover glass holder is available in four sizes of 40, 50, 55, and 60 mm, and a label indicating the applicable size is attached to each holder. Select an appropriate holder according to the length of the cover glass used. Up to 250 cover glasses can be placed in each cover glass holder.

Set the cover glass holder containing cover glasses by orienting the holder in such a way that the side bearing the dimension label faces the front side and the tab of the cover glass holder fits into the holder hole (Figure 4-G).

A label indicating the applicable cover glass type is attached to each cover glass holder.

Figure 4-G

Check the volume of mounting medium in the mounting medium bottle. If the level is low, change the bottle with a new mounting medium bottle.

Raise the handle and place a mounting medium bottle (500 mL) underneath. For best results use the Tissue-

Glas™ Mounting Medium, product code 6419.

Tek

When changing the mounting medium bottle, complete the operation as quickly as possible because air bubbles will enter the tubing if the handle remains raised for an extended period of time.

4.4 Revised 3/30/2010

Page 41

Section 4

Routine Operations

Start of Operation

This section explains the start of operation.

Before instrument operation begins, the following

settings must be checked.

Selecting the Program to Be Used

Select the program suitable for the tissues to be

coverslipped. Every time the < or > key is pressed, the program number changes to display the registered programs one by one.

To change the program parameter, press the MENU

key and perform each change operation. If the program is unlocked, the program details (coverslipping speed, volume of mounting medium) can be changed in real time even during coverslipping. Changes made will be saved under the same program number by overwriting the previous settings.

Checking the Dispense of Mounting Medium

Press the PRIME key to check if mounting medium is dispensed properly. Press and hold the PRIME key for 3 seconds, and mounting medium will be dispensed 10 times consecutively. If mounting medium does not come out, clean the nozzle.

 Operation to change the cover glass size

Press the SIZE key to change the cover glass size.

 Operation to change the dispensing volume

Turn the VOLUME dial to change the dispensing volume.

 Operation to change the coverslipping speed

Tu rn the SPEED dial to change the coverslipping speed.

CAUTION: Confirm that the displayed CG value matches the cover glass actually placed in the instrument.

Revised 3/30/2010 4.5

Page 42

OPERATING INSTRUCTIONS

Placing Slides in a Basket

1. Prepare slides to be coverslipped. Check the stainer station layout and prepare an appropriate basket (20slide basket or 10-slide basket) as well as a basket adapter appropriate for the basket.

2. Attach the basket adapter to the basket in such a way that the “UP SIDE” mark shown on the top of the adapter faces the same direction as the “UP SIDE” mark shown on one side of the basket. After confirming that the two “UP SIDE” marks are facing the same direction, tilt the basket adapter along the groove (Figure 4-H).

3. Next, place the slides into the basket one by one. Set the slides in the correct orientation so that their tissue side aligns with the “UP SIDE” marks. When all slides have been placed, return the tilted basket adapter to the original position.

CAUTION: Use of deteriorated, worn, damaged or deformed baskets and basket adapters can cause problems. Do not use any of these baskets and basket adapters and change deteriorated, worn, damaged, or deformed baskets/adapters with new ones.

CAUTION: Make sure slides are placed in the basket in a balanced manner. If slides are grouped on one side only, the basket may not be transferred properly during staining operation. When there are only a small number of slides, place them carefully by making sure the basket will not tilt.

Tissue side “UP SIDE” mark “UP SIDE” mark

Figure 4-H

CAUTION: Set slides in parallel with the basket partitions. If slides are tilted, they may be damaged (Figure 4-I).

CAUTION: If the basket and basket adapters are not aligned with their “UP SIDE” marks facing the same direction, coverslipping may not be performed and the tissues may sustain significant damage. The misaligned basket and basket adapters can also cause the system to fail. Accordingly, confirm that the basket/adapters are aligned properly. If the tissue side of the slide is not facing the same direction as the “UP SIDE” marks, the slide will be coverslipped on the side where tissues are not mounted. This may cause the tissues to sustain significant damage.

Insert slides in parallel.

Do not tilt slides.

Figure 4-I

4.6 Revised 3/30/2010

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Section 4

Placing a Basket in the Loading Station

1. Confirm that the LOAD lamp on the right side of the control panel is on. While the lamp is not on, baskets cannot be placed.

2. Open the loading station access door, grab the handle, and then slide out the loading station receiver. If the loading station lid is on, remove the lid.

3. Confirm that the loading station is filled with solvent to the specified level (approximately 1.5 L). If there is no solvent or when the solvent level is not sufficient, add solvent.

4. Place the basket in the loading station so that the “UP SIDE” marks on the basket and basket adapter face the right side of the loading station. When placing the basket, be careful not to tilt the basket adapter. If the basket adapter is tilted, the arm may not be able to pick up the basket. The loading station can hold up to 3 slide baskets (Figure 4-J).

Basket Loading

Place the slide in the basket by making sure the tissue side of the slide is facing the same direction as the “UP SIDE” marks (right side). If the slide is set upside down, the cover glass will be coverslipped on the opposite side where tissues are not mounted, and consequently the tissues may sustain significant damage.

Figure 4-J

5. Push back the loading station receiver into the

system and then close the loading station access door (Figures 4-K & 4-L).

Set by making sure the “UP SIDE” marks are facing the right.

Loading station receiver

Figure 4-K

Loading station access door

Figure 4-L

Revised 3/30/2010 4.7

Page 44

OPERATING INSTRUCTIONS

<IN PROCESS> RACK:01 P1:HE-12345 [60mm] [speed:8 volume:100] [STOP] to pause

Starting the Process

After the program has been selected, cover glass,

coverslipping speed and volume of mounting medium have been specified, and a basket has been placed, press the START key or open and close the unloading station door, depending on the start method selected (see page

3.7). Coverslipping will start and the display will switch to

the operation screen.

Figure 4-M: Operation Screen

The coverslipping process is as follows (Figure 4-N):

1. Slides are picked up from the basket by the slide retrieval arm and placed in station 1, one at a time.

2. Slides are moved to station 2 and mounting medium is dispensed.

3. Slides are moved to station 3 and coverslipped with cover glasses.

4. Slides are moved to station 4 and placed in a receiving rack.

5. When all slides have been placed in receiving racks, the receiving racks are moved to the unloading area.

6. The baskets that have become empty following the processing of all slides are placed in the carousel.

7. If any basket remains in the loading station, the process starts over again. If not, coverslipping ends.

4.8 Revised 3/30/2010

Figure 4-N: The Coverslipping Process

Page 45

Section 4

Operations Permitted during Operation

The following operations can be performed during

coverslipping.

• Place and remove receiving racks

• Check the program details

• Change the dispensing volume and coverslipping

speed (These items can be changed only when the program is unlocked.)

• Pause and resume

• Stop the operation

Placing and Removing Receiving Racks

If the RACK lamp is on during operation, it is possible

to place and remove receiving racks in the unloading area.

When the unloading area is opened, the removal

screen appears to show which receiving racks have been there for more than the programmed drying time and can be removed.

If “OK” is lit on the right side of a number, the

receiving rack corresponding to that number can be removed.

If “OK” is blinking on the right side of a number, the

receiving rack corresponding to that number is still being dried (Figure 4-O).

If no drying time is programmed, “OK” is lit next to all

numbers.

Figure 4-O: Retrieval Screen

In the example shown above, rack 1 has been there for more than the drying time and can be removed. Drying of rack 2 is not yet completed.

CAUTION: After opening the door, remove all completed receiving racks.

Place empty receiving racks and remove receiving racks containing slides while the RACK lamp is on. This lamp turns on when the system is stopped or a glass slide is being placed in a receiving rack.

CAUTION: This system returns receiving racks to the positions from which they were retrieved. Accordingly, do not place an empty rack in a position that is empty because the corresponding rack is being filled with slides. Refer to the screen for the available positions.

Checking the Program Details

The current program details are shown in the fourth line when the < and > keys are pressed on the control panel.

Changing the Dispensing Volume and Coverslipping Speed

It is possible to change the dispensing volume and coverslipping speed while the system is processing (assuming the program is not locked) or paused. The new values will be saved under the current program number and reflected in the process.

Change the dispensing volume by turning the VOLUME knob on the control panel.

Change the coverslipping speed by turning the SPEED knob on the control panel.

If either of these settings is changed during processing, the new setting will be in effect from the next slide to be coverslipped.

Revised 3/30/2010 4.9

Page 46

OPERATING INSTRUCTIONS

<IN PROCESS> Please wait

<PAUSE> P1:HE-12345 [60mm] [speed:8 volume:100] [START] [STOP]

Pausing the Process

Resuming the Process

To pause the process, press the STOP key on the

control panel. Note that the process cannot be paused during initial priming or while the dispensing tubing is being cleaned.

While the process is paused, it is possible to navigate menu items, remove receiving racks, change the program, change the cover glass size, perform dispense check, change the dispensing volume, change the coverslipping speed, change the mounting medium bottle.

1. Press the STOP k e y.

2. The pause preparation screen appears (Figure 4-P).

The process does not stop right away, but will

continue until all glass slides currently in the station

have been coverslipped and placed in a rack.

3. When the process is completed, the pause screen

appears and the system will pause (Figure 4-Q). To

resume the operation, press the START ke y. To

cancel the operation, press the STOP k e y.

To resume the paused process, press the START

key. The process will resume. At this time, a caution screen will appear if the loading station access door is open. Close the loading station access door and the process will resume.

Cancelling the Operation

1. To cancel the operation in progress, press the STOP

key.

2. The pause preparation screen appears. The process does not stop right away, but will continue until all glass slides currently in the station have been coverslipped and placed in a rack.

3. When the process is completed, the pause screen appears and the system will pause.

4. Press the STOP key again. The operation stop confirmation screen appears. Press the ENTER key to cancel the operation. Press the EXIT key to return to the pause screen.

Figure 4-P: Pause Preparation Screen

Figure 4-Q: Pause Screen

4.10 Revised 3/30/2010

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Section 4

End of Coverslipping

When all slides present in the loading station have been coverslipped and the receiving racks are ready for removal, a sound is output to notify the end of coverslipping. The system will stop, and will display the standby screen on the control panel. Once the system has stopped, remove the receiving racks from the unloading area. Also remove the empty baskets from the carousel.

Removing the Receiving Racks

1. When the system has stopped completely and the

RACK lamp on the right side of the control panel has

turned on, open the unloading area.

2. Remove the receiving racks containing the slides

(Figure 4-R). Receiving racks containing the slides

are projecting from the recessed area in the

unloading area so that they can be differentiated from

empty receiving racks.

3. When all receiving racks have been removed, place

empty receiving racks in the recessed area and then

close the unloading door.

Concaved part in the unloading area

Figure 4-R

CAUTION: Before moving the coverslipped slides, keep them horizontal and wait until the mounting medium dries thoroughly. Do not position the slides vertically. If the mounting medium is not dry, the slides may stick to the surfaces.

Removing Baskets

After the system has stopped completely, open the cover and remove the baskets from the carousel (Figure 4-S).

Figure 4-S

Revised 3/30/2010 4.11

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OPERATING INSTRUCTIONS

End of Operation

This section explains the operating procedure to be carried out after the system operation has ended.

1. Press the “” side of the power switch to turn off the

power.

2. Open the unloading door and check if any processed

receiving rack remains. Remove all such racks, if any,

and close the unloading door.

3. Open the unloading station access door, grab the

handle, and pull out the unloading station receiver.

Place the lid on the unloading station, push the

unloading station receiver back into the system, and

close the unloading station access door.

NOTE: Performing periodic cleaning and maintenance of

the system during the above end-of-operation processing allows the system to operate in good condition for an extended period of time.

What to Do before Prolonged Period of Non-operation

If the system will not be used for a prolonged period (one week or more), follow the steps below:

1. Turn off the power switch.

2. Disconnect the system power plug from the power

outlet.

3. Open the cover. Remove the cover glass holders and

store them in a dry place.

CAUTION: If the cover glass holders are stored in a humid place, they may cause problems, such as allowing two slides to be picked up together, the next time the holders are used.

4. Add solvent to the anti-drying bottle (Figure 4-T).

Fill solvent until this line just below the lid.

5. Push the lever down to the left (Figure 4-U). The

dispensing nozzle comes down and its tip dips into the liquid in the anti-drying bottle (Figure 4-V).

Dispensing nozzle down lever

Figure 4-U

6. To prevent mounting medium in the dispense tubing

from solidifying, confirm that a mounting medium bottle is placed.

7. Check if a sufficient amount of solvent in the antidrying bottle.

8. Clean the interior of the system.

9. Close the cover.

The tip drops deeper into

the liquid.

Normal state Dispensing nozzle down

lever is pulled

Figure 4-V

Figure 4-T

4.12 Revised 3/30/2010

Page 49

Section 4

Actions to Be Taken upon Power Outage during Operation

This section explains the procedure to be followed when a power outage occurs during operation, as well as the operating procedure to be carried out to reset the system after restoration of power.

Upon Power Outage during Operation

If a power outage occurs during operation, take the necessary action by following the procedure below. In the event of a power outage, even after the power is restored, the operation cannot be resumed from the step the system was performing immediately before the power outage.

1. Press the “” side of the power switch to turn off the

power, in order to prevent the system from moving

suddenly upon restoration of power.

2. After the power has been turned off, open all doors

and covers and take appropriate actions.

• Place the slides not yet coverslipped back in the

solvent, in order to prevent them from drying up.

• Remove the cover glasses that have been picked

up and dropped around the transfer arm, if any.

• Remove the receiving rack currently in the

receiving position, if any.

3. Close all doors and covers, and wait for the power to

restore.

Upon Restoration of Power

After the power has been restored, perform the reset

operation by following the procedure below:

1. Press the “|” side of the power switch to turn on the

power.

2. Once the power is supplied, the operator action screen appears on the control panel to notify that a power outage had occurred.

3. After confirming that no slides remain inside the system, press the EXIT key on the control panel to return to the initial screen.

4. After confirming that the LOAD lamp on the right side of the control panel is on, open the loading station access door, place baskets in the loading station, and then start the operation from the beginning.

CAUTION: The operator action screen notifying the occurrence of power outage also appears after a momentary power failure. In this case, also turn off the system power, remove the half-processed slides and basket, and then start the operation from the beginning.

Revised 3/30/2010 4.13

Page 50

OPERATING INSTRUCTIONS

Coverslipped

Table of Coverslipped Conditions and Operator Actions

To Achieve a Desired Coverslipped Condition

Check the coverslipped slides and if they are not coverslipped in a satisfactory condition, take remedial actions by referring to the table below. For the remedial actions to be taken when two or more cover glasses are picked up together, cover glasses crack when they are picked up, or the like, refer to Section 6, Tr o u bleshooting.

Condition

Large air bubbles are present on slides.

Small air bubbles are present.

Not enough mounting medium is dispensed. Increase the dispense amount of mounting medium.

Air bubbles have entered the dispensing channel of mounting medium, so mounting medium is not dispensed by the specified amount.

Mounting medium has run out.

The cover glass size does not match the applicable settings on the system side.

There is not enough or no clearing agent in the loading station.

The tissue thickness does not match the applicable settings on the system side (amount of mounting medium, coverslipping speed). Settings for amount of mounting medium and/or coverslipping speed need to be adjusted to compensate for the tissue thickness

The coverslipping applicator pressure is too weak and needs to be increased.

Mounting medium did not spread effectively because the ambient temperature was low which increases viscosity and air bubbles entered as a result.

The slide glasses are not of the specified size. Use slide glasses of the specified type.

Mounting medium has solidified on or in the tip of the dispensing nozzle and mounting medium is not being dispensed properly.

Cause Operator Action

Clear mounting medium channel by using the PRIME key or use initial priming operation to remove air bubbles.

Replace the mounting medium bottle with a full one. If the mounting-medium level detection function is not working, the system must be adjusted. Call the Customer Service Center or the Sakura dealer.

Change the cover glass size displayed on the screen according to the size of the cover glass used.

Fill the loading station with clearing agent up to the specified level. If an appropriate amount of clearing agent is not available, mounting medium may not spread properly on the slide and air bubbles may generate as a result.

Increase the amount of mounting medium. Alternatively, decrease the coverslipping speed and examine appropriate coverslipping conditions again. If the tissues do not have an even thickness, air bubbles cannot be fully eliminated.

Increase the applicator pressure of the cover glass during coverslipping. For details, consult the Customer Service Center or the Sakura dealer.

Decrease the coverslipping speed.

Increase the amount of mounting medium. Alternatively, decrease the coverslipping speed and examine appropriate coverslipping conditions again.

Specified slide glass: 26 mm (width) x 76 mm (length) x 0.9 to 1.2 mm (thickness) Adjust the system according to the slide glass used. For

details, consult the Customer Service Center or the Sakura dealer.

Remove the solidified mounting medium.

4.14 Revised 3/30/2010

Page 51

Section 4

Coverslipped

Condition

Small air bubbles are present.

Mounting medium comes out of the slide at the frosted end, etc.

The back of the slide becomes dirty with mounting medium.

Mounting medium is attached to the slide transfer station, causing the slide to not sit properly at the correct angle, so mounting medium is not dispensed properly.

With certain mounting mediums air bubbles enter if the coverslipping speed is low.

The tip of the dispensing nozzle was soaked for an extended period of time in the anti-drying bottle, so the viscosity of the mounting medium in the tip of the dispensing nozzle has dropped.

The viscosity of the mounting medium in the tip of the dispensing nozzle is high. The amount of solvent in the antidrying bottle is low.

There is not enough or no clearing agent in the loading station.

The pressure applying the cover glass during coverslipping is too weak.

The starting position of the application of mounting medium not appropriate.

The amount of mounting medium being dispensed is not appropriate.

Not enough clearing agent remains attached to the slide (when the slide is retrieved from the loading station).

It is normal for mounting medium to come out by several mm or so.

The cover glass size does not match the applicable settings on the system side.

Too much mounting medium is dispensed. Decrease the amount of mounting medium dispensed.

Too much clearing agent remains attached to the slide when the slide is retrieved from the loading station.

The application pressure of the cover glass during coverslipping is too strong.

The starting dispensing position of mounting medium is not appropriate.

The amount of mounting medium dispensed during the first dispense is not appropriate.

Mounting medium from the receiving rack was transferred back to the back of the slide.

The slide transfer stage is dirty with mounting medium, and mounting medium on the slide transfer stage has attached to the back of the slide during transfer.

Too much clearing agent remains attached to the slide (when the slide is retrieved from the loading station).

Cause Operator Action

Remove the attached mounting medium by dissolving it using xylene or other solvent. (If the mounting medium has solidified, remove it using tweezers, etc.)

Decrease the coverslipping speed.

Press the PRIME key to dispense the mounting medium.

Press the PRIME key to dispense the mounting medium. Also adjust the amount of solvent in the anti-drying bottle to an appropriate level.

Add clearing agent to the loading station up to the specified level. If an appropriate amount of clearing agent is not available, mounting medium may not spread properly on the slide and air bubbles may generate as a result.

Increase the cover glass application pressure. For details, consult the Customer Service Center or the Sakura dealer.

Change the start position for application of mounting medium. For details, consult the Customer Service Center or the Sakura dealer.

Change the first dispense amount of mounting medium. For details, consult the Customer Service Center or the Sakura dealer.

Increase the slide retrieval speed. For details, consult the Customer Service Center or the Sakura dealer.

---

Change the cover glass size displayed on the screen according to the size of the cover glass used.

Decrease the slide retrieval speed. For details, consult the Customer Service Center or the Sakura dealer.

Decrease the cover glass application pressure. For details, consult the Customer Service Center or the Sakura dealer.

Change the start position for dispensing of mounting medium. For details, consult our Customer Service Center or the Sakura dealer.

Change the first dispensing amount of mounting medium. For details, consult the Sakura dealer.

Clean the receiving rack with solvent (normally xylene).

Clean the transfer stage with solvent (normally xylene).

Decrease the slide retrieval speed. For details, consult the Customer Service Center or the Sakura dealer.

Revised 3/30/2010 4.15

Page 52

OPERATING INSTRUCTIONS

Coverslipped

Condition

The back of the slide becomes dirty with mounting medium.

The mounting position on the cover glass position deviates.

The cover glass is not covering the tissues on the slide.

The cover slide and slide are not coverslipped parallel.

Suction pad marks remain on the cover glass.

The application pressure of the cover glass during coverslipping is too strong.

The starting dispensing position of mounting medium is not appropriate.

The amount of mounting medium dispensed is not appropriate.

The drying fan is not operating. The system must be adjusted. Contact the Sakura dealer.

Mounting medium of low viscosity was used. Use mounting medium with a viscosity of approx. 500 cps.

The suction pads on the cover glass arm are dirty. Clean the suction pads.

The system is not leveled horizontally.

Too much clearing agent remains attached to the slide (when the slide is retrieved from the loading station).

The drying fan is not operating.

The cover glass holder does not match the cover glass size.

The screws on the pedestal of the cover glass holder are loose.

The cover glass made contact with the receiving rack when the coverslipped slide was placed in the receiving rack, altering the mounting position

The cover glass is too small. Change the cover glasses to a larger size.

The tissue mounting position on the slide is too high or too low.

The slide is set upside down in the basket. Make sure the frosted part is facing up.

The slide is set front-side backwards in the basket.

The cover glass size does not match the applicable settings on the system side. The width is different from the specified value.

The system is not adjusted properly.

The suction pads on the cover glass arm are dirty.

Cause Operator Action

Decrease the cover glass application pressure. For details, consult the Customer Service Center or the Sakura dealer.

Change the start position of dispense of mounting medium. For details, consult the Customer Service Center or the Sakura dealer.

Change the dispensing amount of mounting medium. For details, consult the Customer Service Center or the Sakura dealer.

Use the adjusters on the system to level the system to the installed state. For details, consult the Customer Service Center or the Sakura dealer.

Decrease the slide pickup speed.

The system must be adjusted. For details, consult the Sakura Customer Service Center or the Sakura dealer.

Set cover glasses in cover glass holders of an appropriate size.

The screws must be tightened. For details, contact the Sakura dealer.

The position of the receiving rack must be adjusted. For details, contact the Sakura dealer.

Take note that if the tissues are extremely thick, they may not be fully accommodated in the receiving rack and consequently their mounting position on the cover glass may deviate. Refrain from automatically coverslipping tissues when the slide thickness (slide glass + tissues + mounting medium layer + cover glass) becomes 2 mm or more.

Make sure the position at which tissues are mounted on the slide glass corresponds with the indicated center of the cover glass. (Refer to “Notes on Use” in Appendix C, page C.1). Select an appropriate type of cover glass according to the tissue mounting position.

Make sure the tissue side of the slide is facing the “UP SIDE” mark on the basket.

Use the specified cover glass.

The system must be adjusted. For details, consult the Customer Service Center or the Sakura dealer.

Clean the suction pads.

4.16 Revised 3/30/2010

Page 53

Section 4

How to Operate the g2 when Linked to the Prisma Tissue-Tek

This section explains how to set up the Prisma when the g2 is linked to the Prisma.

How to Set Up the Prisma

1. Select the menu in the bottom right-hand corner of

the Prisma control panel, and then select the Utility

menu tab (Figure 4-W).

2. Select System Setup in the Utility menu (Figure 4-X).

Prisma® Slide Stainer

Figure 4-W

3. Select In Use under Coverslipper Link, and select Tissue-Tek Glas under Coverslipper (Figure 4-Y).

Figure 4-Y

4. Press the EXIT key and select the Stain Process menu tab.

5. In the Stain Process menu, select Staining Mode

Selection (Figure 4-Z).

Figure 4-X

Revised 3/30/2010 4.17

Figure 4-Z

Page 54

OPERATING INSTRUCTIONS

6. Confirm that Yes is selected for Link With

Coverslipper in the System Setup tab (Figure 4-AA).

Figure 4-AA

7. Press the EXIT key to return to the monitor screen.

How to Start from the Prisma

1. Press the STA R T key on the monitor screen.

2. When the display switches to the process start screen (Figure 4-CC), select a staining program and a coverslipping program. When the program number setting key is pressed on the coverslipper, the numeric keypad screen appears (Figure 4-DD). Use the numeric keypad to enter the program number to be used by the coverslipper. “0” is entered by default. If the default setting is used, coverslipping will be performed according to the conditions currently displayed on the coverslipper.

Figure 4-BB

8. Confirm that the link station (LK) is displayed in the bottom left-hand corner of the station layout view.

Figure 4-CC

Figure 4-DD

4.18 Revised 3/30/2010

Page 55

Section 4

3. Press the ENTER key on the numeric keypad to confirm the coverslipping program, and confirm that the program number has been changed (Figure 4EE). Set (save) a coverslipping program for each staining program. When setting a coverslipping program, make sure the applicable program is already programmed on the coverslipper side.

5. After confirming the staining program and the loading

station where the baskets have been placed, press the STA R T key to start the process (Figures 4-GG & 4-HH).

Figure 4-EE

4. Open the Prisma loading station access door, place baskets in the specified loading station, and close the door (Figure 4-FF).

Figure 4-FF

Figure 4-GG

Figure 4-HH

WARNING: MAKE SURE THE SLIDES ARE EVENLY STORED IN THE BASKET. IF THE SLIDES ARE STORED UNEVENLY, THE BASKET MOVEMENT IS AFFECTED DURING THE STAINING OPERATION. TAKE SPECIAL CARE WHEN THERE ARE ONLY A SMALL NUMBER OF SLIDES. STORE THEM CAREFULLY TO PREVENT THE BASKET FROM TILTING.

Revised 3/30/2010 4.19

Page 56

OPERATING INSTRUCTIONS

4.20 Revised 3/30/2010

Page 57

CARE OF THE INSTRUMENT

Section 5

General Maintenance

Keep the exterior of the instrument free of dust at all

times. If needed, the exterior may be cleaned using a damp cloth and a mild detergent; do not use solvents of any kind on the painted exterior parts. The plastic cover may be cleaned with a glass cleaner and soft cloth.

Inspection Methods and Cleaning

To prevent system malfunctions and failures, inspect and clean the system regularly. Unless otherwise specified in this manual, carry out all inspection and cleaning tasks with the power turned off.

Cleaning Each Station and Waste Container

Clean each station and its surrounding area, as well

as each waste container, at the end of each d ay.

1. Open the cover.

2. Brush off each station and its surrounding area and remove dirt and all other foreign matters by collecting them in one location. If it facilitates the process, use adhesive tape to remove foreign matters.

3. Dissolve and remove mounting medium attached

around the stations using a cloth moistened with solvent for mounting medium. If mounting medium has already solidified, remove it using tweezers. Also remove any glass powder. If mounting medium or glass powder attaches to any moving part and subsequently solidifies, an unexpected error or failure may occur.

4. Use a cloth moistened with alcohol to remove oil from the cover glass contacting surfaces of the suction pads on the cover glass arm, and dry the pads.

5. Follow the procedure below to clean the tip of the dispensing nozzle:

• While lifting the dispensing nozzle, move the

nozzle toward the back.

• With the dispensing nozzle still lifted, wipe the tip

using a lint-free cloth.

• Pull out the dispensing nozzle while it is still

lifted, and insert its tip into the anti-drying bottle.

6. Remove each waste container and replace it with a clean container.

7. Check the spatula part of the removed waste

container for deformation or damage. When spatula part is deformed or damaged, replace the waste container with a new container. The spatula of the waste container functions as a drain board for mounting medium at the tip of the dispensing nozzle. If the spatula does not function properly as a drain board, dispensing may be affected.

8. Put solvent for mounting medium in a container and soak the waste container in the solvent for 24 hours to dissolve the attached mounting medium.

9. Remove the waste container and let it dry completely.

10. Close the cover.

Changing Solvent in the Anti-drying Bottle

Change the solvent for mounting medium remaining in the anti-drying bottle with fresh solvent once a week or whenever the solvent in the anti-drying bottle becomes cloudy.

NOTE: Solvent for mounting medium in the anti-drying bottle gets cloudy over time as it is mixed with mounting medium. If the solvent becomes cloudy, the function of the anti-drying bottle, which is to prevent the tip of the dispensing nozzle from drying, may be less effective.

1. Open the cover.

2. Remove the anti-drying bottle while lifting the dispensing nozzle (Figure 5-A).

While lifting the nozzle…

…remove the anti-drying bottle.

Figure 5-A

Revised 3/30/2010 5.1

Page 58

CARE OF THE INSTRUMENT

3. Drain solvent from the anti-drying bottle and add fresh solvent for mounting medium up to the position shown below (Figure 5-B).

4. Return the anti-drying bottle to its original position and close the cover.

Cleaning the Receiving Rack

If the slides are dirty or placed at an angle, mounting medium may be attached the receiving racks (slide racks). Clean each receiving rack once a week or whenever it becomes dirty due to attached mounting medium.

1. Put solvent for mounting medium in a container

made of a material resistant to the solvent, and soak the receiving rack in the solvent for 24 hours to dissolve the attached mounting medium.

2. Remove the receiving rack and let it dry completely.

Cleaning the Loading Station

Tissue fragments, glass powder and other foreign matters collect in the loading station. Accordingly, clean the loading station once a week.

Add solvent to this position below the lid.

Figure 5-B

Adding/Changing Solvent in the Dispensing Pump Reservoir

The dispensing pump reservoir is used to prevent the dispensing pump in the system from locking due to mounting medium. Add solvent if the remaining solvent level drops below the midway point between the shoulder of the reservoir and the line indicating the minimum level. Also change the solvent in the reservoir at least once every two months.

1. Open the cover and remove the lid of the dispensing pump reservoir. To add solvent and go to step 3 . To change solvent, go to step 2.

2. To change solvent, suction out solvent from the dispensing pump reservoir using a pipette.

3. Add solvent for mounting medium up to the shoulder of the dispensing pump reservoir.

4. Place the lid on the dispensing pump reservoir and close the cover.

Cleaning the Slide Arm

Clean the slide arm and slide detection sensor once a week or whenever they become visibly dirty. If the slide arm has become worn or starts experiencing problems picking up slides, the slide arm must be replaced. Contact the Sakura Customer Support Department or the local Sakura distributor.

1. Open the cover.

2. Clean the slide arm and slide detection sensor using a cloth moistened with alcohol.

1. Drain solvent from the basket container.

2. Disassemble the loading station. For the disassembly method, refer to “Installing the Loading Station.”

3. Clean the disassembled parts with a brush or toothbrush by applying the same type of solvent being used in the system.

4. Assemble the loading station.

CAUTION: To minimize contamination, change or filter the solvent in the loading station at the end of each working day.

5.2 Revised 3/30/2010

Page 59

TROUBLESHOOTING

General Information

The following section explains how to recover from conditions that may occur during routine operation. A Troubleshooting Table is provided, which identifies possible conditions related to the electrical and mechanical operations that could occur during routine operation of the Tissue-Tek and remedies are also included so that many isolated problems can quickly be corrected. When dealing with any problem, it is essential to determine which part of the system is the source. A systematic approach should be employed to isolate the problem.

instrument problem, or if the problem cannot be isolated or is beyond the scope of this manual, please contact the Technical Support Department by calling (800) 725-8723, option 2 (U.S. only) or call the local Sakura distributor.

Glass™ g2 Coverslipper. Possible causes

If additional assistance is required concerning an

Section 6

Revised 3/30/2010 6.1

Page 60

TROUBLESHOOTING

6.2 Revised 3/30/2010

Troubleshooting Table

Check the possible problem conditions listed in the table below and if any of them applies to the problem that has occurred, take the specified action or actions. If the problem persists or when none of the listed conditions applies, contact the Sakura dealer.

System condition

Cause

Remedial action

Nothing is displayed.

The power is not turned on.

Turn on the power.

The power plug is disconnected.

Connect the power plug.

The power is not supplied.

Check the breaker on the facility side.

The system power was cut off temporarily

(= momentary power failure).

Turn off the system power and then turn it back on.

Mounting medium is not

dispensed.

The mounting medium bottle is empty.

Replace the mounting medium bottle or add mounting

medium to the specified level or more. If the mountingmedium level detection function is not working, the equipment must be adjusted. Contact the Customer

Service Center or the Sakura distributor.

Mounting medium at the tip of the dispensing

nozzle has solidified.

Dissolve the solidified mounting medium using solvent

(usually xylene). For details, consult the Customer

Service Center or the Sakura distributor.

The system suddenly stopped operating.

A power outage occurred.

Take a remedial action according to “Actions to Be Taken upon Power Outage during Operation” in Section 4.

Cover glasses cannot be

taken out or they dropped

during coverslipping.

The suction pads on the cover glass arm have

deteriorated or are damaged.

Replace the suction pads on the cover glass arm with

new ones. Contact the Customer Service Center or the

Sakura distributor.

Cover glasses are not stored in an appropriate

environment.

Store cover glasses in a place not subject to high humidity. If stored in a humid place, cover glasses may

stick together easily.

Old cover glasses were used.

Use new cover glasses. Old cover glasses may stick

together easily.

Two or more cover glasses

are coverslipped together.

Cover glasses are not stored in an appropriate

environment.

Store cover glasses in a place not subject to high

humidity. If stored in a humid place, cover glasses may

stick together easily.

Old cover glasses were used.

Use new cover glasses. Old cover glasses may stick together easily.

The type of cover glasses used or the angle by

which the cover glass is lifted is not appropriate.

Increase the cover glass lifting angle.

Cover glasses used are not specified for automatic coverslippers.

Use cover glasses specified for automatic coverslippers.

Cover glasses get cracked. The type of cover glasses used or the angle by

which the cover glass is lifted is not appropriate.

Decrease the cover glass lifting angle.

An empty receiving rack is not picked up.

The receiving rack is not set in the specified position correctly.

Set the receiving rack properly in the concaved part of the unloading area.

The receiving rack is damaged.

Contact the Customer Service Center or the Sakura distributor.

The processing capacity drops

and every other slide is skipped. (The CG size on the screen is blinking.)

The cover-glass level detection function was

actuated, and the system switched to the slide pickup mode only after a cover glass was detected.

Press the STOP key and add cover glasses, and the

original processing capacity should be restored. If coverslipping is continued without taking the above action, the cover glass count will drop to 0 and a “No

cover glass” error will generate.

A receiving rack containing coverslipped slides remains during operation, but “OK” is

not displayed on the screen.

When the unloading door was opened, not all receiving racks whose drying time had elapsed (for which “OK” was lit) were taken out.

Open the unloading door, take out all receiving racks containing coverslipped slides and whose drying time has elapsed, and then close the unloading door.

Page 61

Section 6

Revised 3/30/2010 6.3

Actions to Take upon Generation of Errors, Cautions, and Warnings

When an error occurs, an alarm will sound immediately to alert the operator of the abnormality. If coverslipping is still possible, the system will wait until the current process is completed and then display the error screen. Do not touch the system until the error screen appears.

Representative message screens that will appear upon generation of various errors are shown below. Follow the message on the screen to perform up to four steps, remove the slides handled in the current process, and then reset the error.

1. When the error screen below appears (Figure 6-A), be sure to remove all slides on the transfer stage and then press the ENTER key. Store the removed slides in a manner preventing them from drying up, and process them together later on. The removed slides cannot be returned to the baskets in the loading station.

Figure 6-A

2. When the error screen shown below appears (Figure 6-B), press the ENTER key. If the arm is currently gripping a slide, pull out the slide before pressing the ENTER key. Store the removed slides in a manner preventing them from drying up, and process them together later on. If the arm failed to grip a slide, press the ENTER key immediately.

Figure 6-B

3. When the error screen shown below appears (Figure

6-C), retain the cover glass currently being picked up by the suction pads and then press the ENTER key. The cover glass will be released the moment the ENTER key is pressed.

Figure 6-C

4. When the error screen below appears (Figure 6-D),

remove receiving rack from rods by lifting upward to unhook it. Then press the ENTER key.

Figure 6-D

• When slides are in the receiving rack, open the

cover, and carefully remove the receiving rack by raising the rack upward and tilting the rack back towards the left as it is being pulled out so that the slides do not drop out.

• When a rack is being retrieved or unloaded

(when racks are in the unloading area) remove from the area number indicated on screen or visually associated with the position of the rods attached to the robotic arm.

• If rack is still hooked on to the rods, unhook the

rack by lifting upward and tilting the rack slightly back to the left so that the slides do not fall out.

• It might be necessary to reach behind the

receiving area to remove a rack hooked onto the rods.

<ERROR> E562 Remove the rack from the hook [ENTER]for next step

EXXX

<ERROR> E562 MOTOR:Z slide rack [ENTER] to release a cover glass

EXXX

<ERROR> E562 If a slide is on the gripper, remove [ENTER] to next step

EXXX

<ERROR> E562 MOTOR:Z slide rack Remove slides [ENTER]for next step

XXXX

EXXX

Page 62

TROUBLESHOOTING

6.4 Revised 3/30/2010

Page 63

GLOSSARY

Appendix A

Glossary of Terms

40-mm CG:

A cover glass with a length of 40 mm.

50-mm CGC:

A cover glass with a length of 50 mm.

55-mm CG:

A cover glass with a length of 55 mm.

60-mm CG:

A cover glass with a length of 60 mm.

CG:

An abbreviation for “Cover Glass.”

SG:

An abbreviation for “Slide Glass.”

Cover glass lifting angle: When the coverslipping arm lifts each cover glass, it does so at an angle so that multiple cover glasses will not be picked up together. This angle is called the “cover glass lifting angle.”

Basket: A staining basket holding slides that have not yet been coverslipped.

Receiving rack: A rack for holding slides that have been coverslipped.

Loading station: A container in which baskets are placed. Solvent is filled in the loading station to prevent tissues from drying up.

Unloading area:

An area where 12 receiving racks (3 rows x 4 levels) are placed.

Carousel: A disc-shaped storage area for receiving empty baskets.

Anti-drying:

To prevent the tip of the dispensing nozzle from clogging due to mounting medium.

Slide:

A glass slide on which tissues have been pasted.

Storing:

To move a coverslipped slide into a receiving rack.

Coverslipping: To attach a cover glass using mounting medium.

Solvent:

A liquid used to dissolve mounting medium. Xylene is mainly used for this purpose.

Dispense: Dispensing also means applying mounting medium onto a slide as if to draw a straight line.

Revised 3/30/2010 A.1

Page 64

APPENDIX A

A.2 Revised 3/30/2010

Page 65

WARNINGS AND CAUTIONS

Appendix B

Warnings

Do not bring fire sources close to the system

ignite upon contact with fire. Do not bring a fire source within 2 m of the system.

Do not bring fire sources close to the activated carbon filters.

filters may explode upon contact with fire. Do not bring a fire source within 2 m of the activated carbon filters.

Do not disassemble or modify the system.

The system may malfunction or cause an accident.

Do not open covers and doors unless necessary. If the operator must open a cover and insert a hand into the system, do so only after confirming that the system has stopped and the internal components can be operated safely

If a door must be opened and a basket set, do so only after confirming that the LOAD lamp is on and the internal components can be operated safely.

to stop when a cover is opened. However, the actual operation may not stop until the series of steps comprising the operation is completed. Touching the system while it is still operating may result in injury.

. This system uses organic solvent that may

The activated carbon

The system is designed

Do not operate the switches and connect/disconnect the power plug with wet hands. Doing so may result in electric shock.

Connect/disconnect the power plug by holding the plug.

power plug by holding any other part other than the plugs may result in fire or electric shock.

Connecting/disconnecting the

Do not forcibly bend, pull, twist or loop the power cable.

result in fire or electric shock. Should any damage or other abnormality on the power cable or plug be found, contact the Sakura dealer.

Use of a damaged power cable may

Handle reagents (organic solvents) with due care.

this system may be toxic or harmful to the human body. Accordingly, handle these reagents with due care to prevent them from spilling or scattering. Dispose of reagent properly by following the applicable regulation or guideline in your country or region.

Some reagents (organic solvents) used by

When opening/closing any cover, be careful not to pinch your hand.

to pinch your hand between the fixed and moving parts of a cover or between a cover and a door, etc. Your hand may be pinched and injured.

Do not wet the system. Wetting any part of the

system other than those specified in Section 5, “Care of the Instrument” or other applicable sections may cause fire or electric shock due to leak current.

Revised 3/30/2010 B.1

Be careful not

Page 66

APPENDIX B

Cautions

Provide sufficient airing and ventilation around the system.

(organic solvent) that is toxic and harmful to the human

body. Provide sufficient airing and ventilation around the

system.

Close the lids on containers during operation.

this system may be toxic or harmful to the human body. If

containers of these reagents remain without lids for many

hours, organic gases may fill up the room.

Some reagents (organic solvents) used by

Turn off the power before performing cleaning.

supplied may result in unexpected accidents.

Cleaning the system while the power is

Connect the power plug to a power outlet with grounding terminal.

to a power outlet with grounding terminal conforming to at

least the Class D (former Class 3) grounding specification.

If the system is not grounded, fire or electric shock may

result due to leak current.

In the event of system malfunction, turn off the power and then disconnect the power plug from the power outlet.

event of system malfunction, turn off the power and

contact the Sakura Service Center. Disconnecting the

power plug from the power outlet is one way to cut off the

main system power. In the event of abnormality,

disconnect the power plug from the power outlet. When

installing the system, select a suitable location where the

power outlet will not be concealed and the power plug can

be disconnected immediately in case of emergency.

This system uses solvent

Connect the power plug

In the

Do not touch the moving parts of the system.

operating may result in injury.

Inspect the system once every six months.

Conduct a periodic inspection every six months to ensure

that the system will be used safely and continue to

demonstrate the desired performance. Contact the Sakura

dealer for the details of periodic inspection.

Exercise due caution when handling reagent. Wear gloves, mask and/or safety goggles or take other proper measures designed to protect the operator, by following the applicable regulation or guideline in your country or region.

reagents are toxic and harmful to the human body.

Touching moving parts while the system is

Some

If any substance that presents biological hazards is handled, ensure safety by following the applicable regulation or guideline in your country or region.

Ask the dealer to install or relocate the system.

expert knowledge of the system may result in unexpected

accidents.

Installation of the system by people having no

Do not remove the warning/caution labels.

Without these labels, the operators cannot be reminded of

the necessary warning/cautionary information in the day-

to-day handling of the system, which may result in

unexpected problems.

B.2 Revised 3/30/2010

Page 67

NOTES ON USE

Notes on Use

Read this “Notes on Use” section beforehand to

ensure the correct use of the system. This section explains the items that should be heeded to prevent system failures, as well as items that should be noted to perform proper coverslipping operation.

Cautions for Preventing system Failure

Do not place any object on the system.

Doing so may result in unexpected problems.

Do not place any object on a moving part.

Doing so may result in system failure.

Appendix C

Do not spill any organic solvent onto the system.

Do not use toluene.

system malfunction or failure.

Use designated consumable parts.

any other consumable part may result in malfunction or

system failure.

Use of toluene may cause

Use of

Do not block the exhaust outlet. When the

exhaust outlet on the left side at the rear of the system is

blocked, fumes may not be exhausted properly.

Do not use any ultraviolet sterilizing lamp around the system. Ultraviolet light may cause the

system characteristics to change or deteriorate.

When opening/closing a cover or door, do so slowly.

door, the cover/door may be damaged.

If large impact is applied to a cover or

Avoid using a deteriorated or damaged basket or receiving racks.

in malfunction or failure.

Doing so may result

Do not open any cover or door unless necessary.

required in the applicable operation specified in this

manual. Once opened, the cover/door should be closed as

soon as the operation is finished.

Revised 3/30/2010 C.1

Open each cover or door only when

Page 68

APPENDIX C

Notes for Proper Coverslipping Operating

Put a sufficient amount of clearing agent (mainly xylene) in the loading station to prevent tissues from drying up.

Determine the operating conditions after a coverslipping test.

system vary depending on the tissues on the slide.

Conduct a physical coverslipping test using the system

before determining the operating conditions.

Paste tissues at the center of the pasting position on the cover glass.

not provide a function for checking the position of pasted

tissues on the glass slide. When pasting tissues on the

glass slide, make sure tissues are aligned at the center of

the cover glass (indicated by *) by taking into account the

settings of the cover glass used.

The shaded area indicates the pasting position on each cover glass.

40-mm CG 50-mm CG

The operating conditions of the

This system does

55-mm CG 60-mm CG

If very thick tissues are coverslipped in some positions, the cover glass may not be stored in the receiving rack properly. Do not use very thick tissues with this system.

C.2 Revised 3/30/2010

Sakura Tissue-Tek Glas g2 Operating Manual

Specifications and Main Features

Frequently Asked Questions

User Manual