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Protease Inhibitor Cocktails
Art. No.
3743
3749
3751
3755
3758
3760
Inhibitor
Cocktail
Standard
BroadRange
Plus
Tissue
Bacteria
His-tag
Sipment
lyophilisiert
(uncooled)
lyophilisiert
(uncooled)
in 1 ml DMSO
(cooled)
lyophilisiert
(uncooled)
lyophilisiert
(uncooled)
in 1 ml DMSO
(cooled)
Storage
-20 °C
-20 °C
-20 °C
-20 °C
-20 °C
-20 °C
Dilute in
100 ml
H2O dist. or
extract. buffer
1 ml H2O dist.
-
1 ml H2O dist.
1 ml DMSO
+ 4 ml H2O dist.
-
Stock
Solution
-*
ca. 100x
for 20 g
ca. 100x
(for 2 g)
ca. 40x
(for 20 g E. coli)
ca. 100x
(for 10 g)
Effectual for
100 ml extr.
buffer
1 - 2 g cells/
tissue
20 g cells/ tissue
ca. 2 g cells/
tissue
20 g E. coli
10 g bacterial
cells
Store Stock
4 °C
-20 °C
-20 °C
-20 °C
-20 °C
-20 °C
Shelf Life
90 days
1 months
6 months
6 months
6 months
6 months
Inhibitor
conc. in
1 x solution
1 ml stock
solution
1 ml stock
solution
1 ml stock
solution
5 ml stock
solution
1 ml stock
solution
AEBSF
1.67 mM
50 mM
100 mM
50 mM
20 mM
100 mM
Aprotinin
-
15 µM
80 µM
15 µM - -
Bestatin - -
5 mM
-
1.7 mM
5 mM
E-64 - 100 µM
1.5 mM
100 µM
200 µM
1.5 mM
EDTA
13.44 mM
50 mM - -
85 mM
-
Leupeptin
2.1 µM
100 µM
2 mM
100 µM - -
Pepstatin A
1.46 µM
-
1 mM
-
2 mM
0.2 mM
Phosphoramidon
- - - - -
2 mM
Application
Solubilisation of
the cocktail
directly in
extraction buffer
Add 10 µl stock
sol. to 1 ml
extract (max.
20 mg tissue)
Add 10 µl stock
sol. to 1 ml
extract (max.
200 mg tissue)
Add 10 µl stock
sol. to 1 ml
extract (max.
20 mg tissue)
Add 25 µl stock
sol. to 1 ml
extract (max.
100 mg E. coli)
Add 10 µl stock
sol. to 1 ml
extract (max.
100 mg bacter.)
* Due to solubility and high inhibitor concentrations in this cocktail, stock solutions of Inhibitor Cocktail Standard are hard to prepare.
A 10x stock solution may be prepared by solubilisation in 10 ml H2O, it is, however, not recommended.
Hazard and Precautionary Statements:
Please note safety data given on label and MSDS
.
Instructions for use
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Inhibitor Cocktail Standard 1 tube, plastic. 3743.1
Inhibitor Cocktail BroadRange 1 tube, plastic. 3749.1
Inhibitor Cocktail Plus 1 ml, plastic. 3751.1
Inhibitor Cocktail Tissue 1 tube, plastic. 3755.1
Inhibitor Cocktail Bacteria 1 kit, plastic. 3758.1
Inhibitor Cocktail His-tag 1 ml, plastic. 3760.1
Carl Roth GmbH + Co. KG
Schoemperlenstraße 3-5 • 76185 Karlsruhe
P.O. Box 100121 • 76231 Karlsruhe
Phone: +49 (0) 721/ 5606-0
Fax: +49 (0) 721/ 5606-149
info@carlroth.com • www.carlroth.com sse 06/2021
The company is a limited partnership with headquarters in Karlsruhe, reg. court
Mannheim HRA 100055. Roth Chemie GmbH, with headquarters in Karlsruhe, reg.
court Mannheim HRB 100428, is the personally liable partner. Managing Director:
André Houdelet. Sales tax identification number: DE 143621073.
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