Q Imaging Retiga 3000 User Manual

www.QIMAGING.com

Retiga 3000 USER MANUAL

Applicability

This document applies to the Retiga™ 3000 camera. For the latest updates, please visit www.qimaging.com.

Notice of Copyright

Trademarks and Proprietary Names

QCapture Pro™, and Retiga™ are trademarks of QImaging. PVCAM® and QImaging® are registered trademarks of Roper Scientiﬁc, Inc. Product names mentioned in this document may be trademarks or registered trademarks of QImaging or other hardware, software or service providers, and are used herein for identiﬁcation purposes only. Microsoft® and Windows® are registered trademarks in the U.S. and other countries of Microsoft Corporation and are used herein for identiﬁcation purposes only.

QImaging Corporation Address Information

19535 56th Avenue, Suite 101 Surrey, BC, Canada V3S 6K3

604.530.5800

www.qimaging.com

QImaging Technical Support

Technical support is available to all registered users of QImaging products from 9am to 5pm Pacific Standard Time.

support@qimaging.com

800.874.9789

http://www.qimaging.com/support/contact

Retiga 3000 USER MANUAL

QIMAGING LIMITED WARRANTY

Standard Product Warranty Plan

A Standard Product Warranty Plan is included with every QImaging camera purchase. This Warranty Plan includes parts and labor for two full years (starting from the shipping date of the camera). The Standard Product Warranty Plan is provided on all new and used equipment, including retired demonstration cameras.

Extended Product Warranty Plan

Extended Product Warranty Plans are conveniently priced and very easy to purchase. Available for all QImaging cameras, the Extended Product Warranty Plan includes parts and labor, and is available in one-year increments up to ﬁve years.

When you purchase an Extended Product Warranty Plan from QImaging, you are assured of our commitment to minimizing down times. Your needs are our top priority and QImaging responds immediately. The QImaging Service and Support team is focused on expediting all customer requests to provide a fast and complete solution.

QImaging also offers on-site training as well as online operational training programs. These programs are designed to get you up and running with your new camera quickly and efﬁciently.

Contact a QImaging Representative to learn more about Extended Product Warranty Plan options.

RETIGA 3000 CERTIFICATE OF CONFORMITY

Retiga 3000 USER MANUAL

Table of Contents

INTRODUCTION ............................................................................................................. 1

System Components ........................................................................................................1

Precautions ........................................................................................................................2

Environmental Conditions for Operation and Storage ......................................................... 2

Microscopes, Lenses, Tripods .............................................................................................. 2

Cleaning ............................................................................................................................3

Repair ................................................................................................................................3

INSTALLATION ............................................................................................................... 4

Host Computer Requirements ............................................................................................4

Camera Power Requirements .............................................................................................5

Installation Step 1: Install the USB 3.0 PCIe Card ................................................................5

Installation Step 2: Install the Camera Driver .......................................................................5

Installation Step 3: Connect the Camera ...........................................................................6

USING YOUR RETIGA 3000 CAMERA ............................................................................ 7

Imaging Software ............................................................................................................... 7

Basic Camera Parameters ...................................................................................................7

Exposure Time ....................................................................................................................7

Gain State ......................................................................................................................... 7

Sensor Cooling ...................................................................................................................8

Clocking Mode ..................................................................................................................8

Clearing Mode ................................................................................................................... 9

ROI / Binning .................................................................................................................... 10

Device Synchronization ...................................................................................................10

TROUBLESHOOTING .................................................................................................... 13

Resolving Problems with the Camera ................................................................................ 13

Unresolved Problems - Contacting QImaging Support ......................................................14

SPECIFICATIONS ........................................................................................................... 15

Camera Dimensions (Front) ..............................................................................................15

Camera Dimensions (Bottom) ........................................................................................... 15

Additional Camera Measurements ...................................................................................15

Connectors on Back of Camera ........................................................................................ 16

Power Supply Speciﬁcations .............................................................................................16

Focal Plane Measurement ................................................................................................. 17

Sensor Speciﬁcations ....................................................................................................... 17

Sensor Orientation ...........................................................................................................18

I/O Connector Pinout .......................................................................................................18

APPENDIX A: USB Information ................................................................................... 20

USB 2.0 Chipset Incompatibility Notice ............................................................................. 20

Determining if a USB port is on a Root Hub ...................................................................... 21

CHAPTER 1

INTRODUCTION

The Retiga 3000 is the ideal camera for high resolution snapshot ﬂuorescence documentation. With 2.8 million pixels, a

4.54μm pixel pitch and a high quantum efﬁciency of 75%, this camera provides higher resolution and sensitivity than most standard ﬂuorescence microscopy cameras. Available in monochrome or color, the Retiga 3000 uses a USB 2.0 data connection for a fast and easy installation. Additionally, low-noise electronics, cooled sensor technology, and excellent quantum efﬁciency (QE) yield superb sensitivity for low-light ﬂuorescence applications.

This manual describes the installation and conﬁguration procedures for your new Retiga 3000 color or monochrome camera. Descriptions of Retiga 3000 camera settings are also included.

System Components

Your new camera system includes:

■

Retiga 3000 camera

■

5V 4A power supply

■

1 Meter USB 2.0 cable

■

Black lens cap

■

USB ﬂash drive: PVCAM driver, QCapture Pro installer, manual, datasheet, training materials

■

USB security key (required for QCapture Pro software)

■

StarTech 2 Port PCI Express (PCIe) SuperSpeed USB 3.0 Card Adapter

Retiga 3000 USER MANUAL

Precautions

The CCD and other Retiga 3000 camera system electronics are extremely sensitive to electrostatic discharge (ESD). To avoid permanently damaging the system, please observe the following precautions:

■

If you are using high-voltage equipment (such as an arc lamp) with your camera system, be sure to turn the camera power on last and power the camera off ﬁrst.

■

Never connect or disconnect any cable while the camera system is powered on.

■

Although you should turn off the camera’s power supply before disconnecting any camera system cable, you do not need to power off your computer to detach the cables.

■

Use caution when triggering high-current switching devices (such as an arc lamp) near your system. The CCD can be permanently damaged by transient voltage spikes. If electrically noisy devices are present, an isolated, conditioned power line or dedicated isolation transformer is highly recommended.

■

Always leave at least one inch of space around the camera housing.

■

Never open the camera. There are no user-serviceable parts inside the Retiga 3000 camera. Opening the camera voids the warranty.

■

Use only the integrated USB 2.0 interface, cables, and power supply designated for this camera system. Using non-Retiga 3000 cables or power supplies may result in permanent damage to your system.

■

Do not use a C-mount lens that has optics that extend behind the ﬂange of the lens.

Environmental Conditions for Operation and Storage

Your Retiga 3000 camera system should be operated in a clean, dry environment. The camera system’s ambient operating temperature is 0°C to 30°C with 80% relative humidity, noncondensing.

Store the Retiga 3000 camera system in its original containers. To protect the system from excessive heat, cold, and moisture, store at an ambient temperature between -20°C and 60°C with a relative humidity of 0% to 90%, noncondensing.

Microscopes, Lenses, Tripods

Retiga 3000 has a standard threaded video mount and can be mounted to any microscope that accepts a standard C-mount adapter. The camera also allows you to install any lens that is compatible with a standard threaded video mount as long as its optics do not extend behind the ﬂange of the lens.

The Retiga 3000 camera can be mounted to a tripod using the tripod mounting attachment located on the bottom of the camera. See the Speciﬁcations chapter for more information.

Retiga 3000 USER MANUAL

Cleaning

Clean the exterior surfaces of the camera with a dry, lint-free cloth. To remove stains, contact QImaging Support. To clean the camera’s imaging window, use only a ﬁltered compressed-air source. Handheld cans are not recommended, as they may spray propellant onto the window. Do not touch the window.

Repair

The Retiga 3000 camera system contains no user-serviceable parts. Repairs must be done by QImaging. Should your camera system need repair, contact QImaging Support. Please save the original packing materials so you can safely ship the camera system to another location or return it for repairs if necessary.

IMPORTANT: DO NOT OPEN the camera. Opening the Retiga 3000 camera voids the warranty.

Retiga 3000 USER MANUAL

CHAPTER 2

INSTALLATION

This chapter will detail the proper installation of your Retiga 3000 camera. In order to install and use the camera, the following items are required:

■

Retiga 3000 camera (supplied)

■

USB 2.0 data cable (supplied)

■

5V 4A power supply (supplied)

■

USB “installer” ﬂash drive (supplied)

■

StarTech 2 Port PCIe SuperSpeed USB 3.0 Card Adapter (supplied)

■

110V/220V power connection

■

A PC that meets host computer requirements

Host Computer Requirements

The host computer for your Retiga 3000 camera must include:

■

Windows 7 operating system (32/64 bits)

■

2.0 GHz or faster Intel processor: either Xeon or Core i5

■

4+ GB RAM

■

250+ GB serial ATA (SATA) HDD and/or >64 GB solid state drive (SDD) for high-speed imaging and storage

■

256+ MB slot-based ATI/NVIDIA video graphics card (i.e., not an “onboard/integrated graphics” adapter)

■

CD-ROM drive or internet access to install the driver

■

One available PCIe slot

NOTE: Minimum requirements as of January 2014. Supported computer systems will change as new cameras, computer hardware, and operating systems are introduced and older models then become obsolete. For current information on recommended computer speciﬁcations, please visit:

http://www.qimaging.com/support/recommended-computer-speciﬁcations.php

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Q Imaging Retiga 3000 User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual