QIAGEN QuantiFERON Instruction Manual

Download

Sample to Insight__

626410

www.qiagen.com

QIAGEN

1122892

QuantiFERON

ELISA Instructions for Use (Handbook)

Human IFN-γ ELISA test

November 2020

2 x 96

For Research Use Only Not for use in diagnostic procedures

, 19300 Germantown Road, Germantown, MD 20874, USA

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Contents

Intended Use .............................................................................................................. 5

Principle of the Procedure ............................................................................................ 6

Time required to perform the assay ..................................................................... 6

Materials Provided ...................................................................................................... 7

Kit contents ...................................................................................................... 7

Materials required but not provided .............................................................................. 8

Warnings and Precautions ........................................................................................... 9

Safety information ............................................................................................ 9

Precautions ...................................................................................................... 9

Storage and Handling ............................................................................................... 12

ELISA kit reagents ........................................................................................... 12

Reconstituted and unused ELISA reagents .......................................................... 12

Procedure ................................................................................................................ 13

Interpretation of Results .............................................................................................. 18

Generation of standard curve .......................................................................... 18

Quality control of test ...................................................................................... 19

Analytical performance ................................................................................... 20

Technical Information ................................................................................................ 22

Clotted plasma samples .................................................................................. 22

Troubleshooting Guide .............................................................................................. 23

Symbols ................................................................................................................... 25

Contact Information .................................................................................................. 26

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Appendix A – Abbreviated Test Procedure ................................................................... 27

Ordering Information ................................................................................................ 29

Document Revision History ......................................................................................... 30

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Intended Use

The QuantiFERON ELISA (QFN) is for research use only to detect human interferon gamma (IFN-γ) in plasma by enzyme-linked immunosorbent assay (ELISA).

The QuantiFERON ELISA can be used to detect IFN-γ in plasma generated from QuantiFERON Blood Collection Tubes.

The QFN ELISA is not for use in diagnostic procedures.

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Principle of the Procedure

The concentration of IFN-γ in a human plasma sample reported in International Units per ml (IU/ml) is measured by QuantiFERON ELISA.

Time required to perform the assay

The time required to perform the QFN ELISA is estimated below. The time of testing multiple samples when batched is also indicated.

 ELISA Approx. 3 hours for one ELISA plate

(up to 88 samples) <1 hour labor Add 10–15 minutes for each extra plate

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Catalog no.

Materials Provided

Kit contents

QuantiFERON® 2 Plate Kit ELISA

Microplate Strips,12 x 8 wells

IFN-γ Standard, lyophilized

Green Diluent

Conjugate 100x Concentrate, lyophilized

Wash Buffer 20x Concentrate 1 x 100 ml

Enzyme Substrate Solution 1 x 30 ml

Enzyme Stopping Solution* 1 x 15 ml

Language Sheet 1

2 Plate kit ELISA

626410

2 sets 12 x 8-well Microplate Strips

1 x vial (8 IU/ml when reconstituted)

1 x 30 ml vial

1 x 0.3 ml, when reconstituted

* Contains sulfuric acid. See Warnings and Precautions.

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Materials required but not provided

 Calibrated variable-volume pipets*  Calibrated multichannel pipet* capable of delivering 50 µl and 100 µl with disposable

tips

 Microplate shaker†  Deionized or distilled water, 2 liters  Microplate washer (automated washer recommended)  Microplate reader† fitted with 450 nm filter and 620 nm to 650 nm reference filter  Graduated cylinder (measuring cylinder)  Low-lint absorbent towels  Plate lid

* Ensure that the instruments have been checked and calibrated according to the manufacturer’s recommendations.

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Handle human plasma as if potentially infectious.

products in accordance with federal, state, and local

Warnings and Precautions

Safety information

For Research Use Only. Not for use in diagnostic procedures.

When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. For more information, please consult the appropriate safety data sheets (SDSs). These are available online in convenient and compact PDF format at www.qiagen.com/safety where you can find, view, and print the SDS for each QIAGEN kit and kit component.

Precautions

CAUTION

Observe relevant blood and blood product handling guidelines. Dispose of samples and materials in contact with blood or blood

regulations.

(C1)

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

QuantiFERON Enzyme Stopping Solution

Contains: sulfuric acid. Warning! May be corrosive to metals.

QuantiFERON Enzyme Substrate Solution

Warning! Causes mild skin irritation. Wear protective

Contains: tartrazine. Warning! May cause an allergic skin

QuantiFERON Wash Buffer 20x Concentrate

Harmful to aquatic life with long lasting effects. Avoid release

Hazard statements

The following hazards and precautionary statements apply to components of the

QuantiFERON ELISA.

Causes skin irritation. Causes serious eye irritation. Wear protective gloves/ protective clothing/ eye protection/ face protection.

gloves/ protective clothing/ eye protection/ face protection.

QuantiFERON Green Diluent

reaction. Wear protective gloves/ protective clothing/ eye protection/ face protection.

to the environment.

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Further information

 Do not use the ELISA kit if any reagent bottle shows signs of damage or leakage prior to

use.

 Do not mix or use the Microplate Strips, IFN-γ Standard, Green Diluent, or Conjugate

100x Concentrate from different QFN ELISA batches. Other reagents (Wash Buffer 20x Concentrate, Enzyme Substrate Solution, and Enzyme Stopping Solution) can be interchanged among kits, providing the reagents are within their expiration periods and lot details are recorded.

 Discard unused reagents and biological samples in accordance with local and national

safety and environmental regulations.

 Do not use the QFN ELISA after the expiration date.  Make sure that laboratory equipment has been calibrated/validated for use.

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Storage and Handling

ELISA kit reagents

 Store ELISA kit reagents at 2–8°C.  Always protect Enzyme Substrate Solution from direct sunlight.

Reconstituted and unused ELISA reagents

For more information about how to constitute the ELISA reagents, see Procedure.

 The reconstituted kit standard may be kept for up to 3 months if stored at 2–8°C.  Note the date on which the kit standard was reconstituted.  Once reconstituted, unused Conjugate 100x Concentrate must be returned to storage at

2–8°C and must be used within 3 months.

 Note the date on which the conjugate was reconstituted.  Working strength conjugate must be used within 6 hours of preparation (see Table 1).  Working strength wash buffer may be stored at room temperature (22°C ± 5°C) for up to

2 weeks.

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Procedure

Materials required

 QuantiFERON ELISA 2 Plate Kit*

Materials required but not provided

 See Materials required but not provided.

Important points before starting

 All plasma samples and reagents, except for Conjugate 100x Concentrate, must be

brought to room temperature (22°C ± 5°C) before use. Allow at least 60 minutes for equilibration.

Procedure

1. Remove strips that are not required from the microplate frame, reseal in the foil pouch, and return to the refrigerator for storage until needed.

Note: Allow at least one strip for the QFN standards and sufficient strips for the number of subjects being tested. After use, retain frame for use with remaining strips.

2. Reconstitute the lyophilized IFN-γ Standard with the volume of deionized or distilled water indicated on the label of the Standard vial. Mix gently to minimize frothing and ensure complete solubilization.

Note: Reconstitution of the Standard with the stated volume will produce a solution with a concentration of 8.0 IU/ml. The Standard can be used to generate an 8-point Standard Curve or a 4-point Standard Curve. Each dilution within the standard curve should be run in at least duplicate.

* See Materials Provided.

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Important: The reconstitution volume of the IFN-γ Standard differs between batches. Consult the label of the standard vial to ensure that you use the correct volume of deionized or distilled water.

Use the reconstituted Kit Standard to produce a dilution series of 8 IFN-γ concentrations (see Preparation of 8-point standard curve and Figure 1), or a dilution series of 4 IFNγ (see Preparation of 4-point standard curve and Figure 2). If a dilution series other than that described is used, each laboratory should validate the alternative approach.

Note: Prepare fresh dilutions of the Kit Standard for each ELISA session (Figures 1 and 2)

 Preparation of 8-point standard curve

 Add 300 µl of the Kit Standard to tube labeled as Standard 1.  Add 150 µl of Green Diluent to 7 tubes (labeled Standard 2 to Standard 8)  To perform serial dilutions, transfer 150 µl of each standard to the next tube. Mix

each tube thoroughly before the next transfer.

 The undiluted Kit Standard serves as the highest concentration.

Figure 1. Preparation of 8-point standard curve

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

 Preparation of 4-point Standard Curve

 Label 4 tubes as S1, S2, S3, and S4.  Add 150 µl of Green Diluent (GD) to S1, S2, S3, and S4.  Add 150 µl of the Kit Standard to S1. Mix thoroughly.  Transfer 50 µl from S1 to S2. Mix thoroughly.  Transfer 50 µl from S2 to S3. Mix thoroughly.  Green Diluent alone serves as the zero standard (S4).

Figure 2. Preparation of 4-point standard curve

3. Reconstitute lyophilized Conjugate 100x Concentrate with 0.3 ml of deionized or distilled water. Mix gently to minimize frothing and ensure complete solubilization of the conjugate.

Note: Working strength conjugate is prepared by diluting the required amount of reconstituted Conjugate 100x Concentrate in Green Diluent (Table 1). Return any unused Conjugate 100x Concentrate to 2–8°C immediately after use. Use only Green Diluent.

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Table 1. Conjugate preparation

Number of strips Volume of concentrate

100x concentrate

2 10 µl 1.0 ml

3 15 µl 1.5 ml

4 20 µl 2.0 ml

5 25 µl 2.5 ml

6 30 µl 3.0 ml

7 35 µl 3.5 ml

8 40 µl 4.0 ml

9 45 µl 4.5 ml

10 50 µl 5.0 ml

11 55 µl 5.5 ml

12 60 µl 6.0 ml

Volume of Green Diluent

4. For plasma samples subsequently stored or frozen, mix samples before addition to the ELISA well.

5. Add 50 µl of freshly prepared, working strength conjugate to the required ELISA wells using a multichannel pipet.

6. Add 50 µl of test plasma sample to appropriate wells using a multichannel pipet. Then, add 50 µl of each of standards 1 to 8, or 1 to 4; depending on the standard curve format used. Assay the standards in at least duplicate.

7. Cover each plate with a lid and mix the conjugate and plasma samples/standards thoroughly using a microplate shaker for 1 minute. Avoid splashing.

8. Incubate at room temperature (22°C ± 5°C) for 120 ± 5 minutes.

Note: Plates should not be exposed to direct sunlight during incubation.

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

9. During incubation, dilute 1 part Wash Buffer 20x Concentrate with 19 parts deionized or distilled water and mix thoroughly. Sufficient Wash Buffer 20x Concentrate has been provided to prepare 2 liters of working strength wash buffer.

Note: Wash wells with 400 µl of working strength wash buffer for at least 6 cycles in a microplate washer. An automated plate washer is recommended.

Important: Thorough washing is very important to the performance of the ELISA. Make sure each well is completely filled with wash buffer for each wash cycle. It is recommended to soak wells for a period of at least 5 seconds between each cycle for best results.

Add standard laboratory disinfectant to the effluent reservoir and follow established procedures for the decontamination of potentially infectious material.

10. Tap plates face down on absorbent, low-lint towel to remove residual wash buffer. Add 100 µl of Enzyme Substrate Solution to each well, cover each plate with a lid, and mix thoroughly using a microplate shaker.

11. Incubate at room temperature (22°C ± 5°C) for 30 minutes.

Note: Plates should not be exposed to direct sunlight during incubation.

12. Following incubation, add 50 µl of Enzyme Stopping Solution to each well and mix thoroughly using a microplate shaker.

Note: Enzyme Stopping Solution should be added to wells in the same order and at approximately the same speed as used when adding the Enzyme Substrate Solution in step 13.

13. Measure the Optical Density (OD) within 5 minutes of stopping the reaction using a microplate reader fitted with a 450 nm filter as well as a 620–650 nm reference filter. OD values are used to calculate results.

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Interpretation of Results

QuantiFERON R&D Analysis Software is used to analyze raw data and calculate results. It is available at www.qiagen.com. Ensure that the most current version of the QuantiFERON R&D Analysis Software is used.

The software performs a quality control assessment of the ELISA, generates a standard curve, and provides a test result in IU/ml for each sample, as detailed in the Results section.

Generation of standard curve

As an alternative to using the QuantiFERON R&D Analysis Software, results can be determined using this method.

Determine the mean OD values of the kit standard replicates on each plate.

Construct a log (y-axis) against the log

(e)

-log

standard curve by plotting the log

(e)

of the IFN-γ concentration of the standards in IU/ml

(e)

of the mean OD

(e)

(x-axis), omitting the zero standard from these calculations. Calculate the line of best fit for the standard curve by regression analysis.

Use the standard curve to determine the IFN-γ concentration (IU/ml) for each of the test plasma samples, using the OD value of each sample.

These calculations can be performed using software packages available with microplate

readers and standard spreadsheet or statistical software (such as Microsoft

Excel® software). It is recommended that these packages be used to calculate the regression analysis, the coefficient of variation (%CV) for the standards, and the correlation coefficient (r) of the standard curve.

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Quality control of test

The accuracy of test results is dependent on the generation of an accurate standard curve. Therefore, results derived from the standards must be examined before test sample results can be interpreted.

For the ELISA to be valid:

8-point Standard

 The mean OD value for Standard 1 must be ≥1.200.  The %CV for Standards 1 to 5 replicate OD values must be ≤15%.  Replicate OD values for Standards 6 to 8 must not vary by more than 0.040 optical

density units from their mean.

 The correlation coefficient (r) calculated from the mean absorbance values of the

standards must be ≥0.98.

4-point Standard

 The mean OD value for Standard 1 must be ≥0.600.  The %CV for Standard 1 and Standard 2 replicate OD values must be ≤15%.  Replicate OD values for Standard 3 and Standard 4 must not vary by more than 0.040

optical density units from their mean.

 The correlation coefficient (r) calculated from the mean absorbance values of the

standards must be ≥0.98.

The QuantiFERON R&D Analysis Software calculates and reports these quality control parameters.

If the above criteria are not met, the run is invalid and must be repeated.

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

The mean OD value for the zero standard (Green Diluent) should be ≤0.150. If the mean OD value is >0.150, the plate washing procedure should be investigated.

Analytical performance

The QFN ELISA uses a recombinant human IFN-γ standard, which has been assayed against a reference IFN-γ preparation (NIH; Ref: Gxg 01-902-535). Results for test samples are reported in International Units (IU) relative to a standard curve prepared by testing dilution of the secondary standard supplied with the kit.

Heterophile (e.g., human anti-mouse) antibodies in serum or plasma of certain individuals are known to cause interference with immunoassays. The effect of heterophile antibodies in the QFN ELISA is minimized by the addition of normal mouse serum to the Green Diluent and the use of F(ab’)2monoclonal antibody fragments as the IFN-γ capture antibody coated to the microplate.

The QFN ELISA has been demonstrated to be linear by placing 5 replicates of 11 plasma pools of known IFN-γ concentrations randomly on the ELISA plate. The linear regression line has a slope of 1.002 ± 0.011 and a correlation coefficient of 0.99 (Figure 6).

The limit of detection of the QFN ELISA is 0.065 IU/ml and there is no evidence of a highdose hook (prozone) effect with concentrations of IFN-γ up to 10,000 IU/ml.

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Figure 3. Linearity profile of QFN ELISA determined from testing 5 replicates of 11 plasma samples of known IFN-γ concentrations.

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Technical Information

Clotted plasma samples

Should fibrin clots occur with long-term storage of plasma samples, centrifuge the samples to sediment clotted material and facilitate pipetting of plasma.

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Comments and suggestions

a) Incomplete washing of the

Wash the plate at least 6 times with 400 µl/well of wash buffer. More than 6 b) Cross-contamination of ELISA

Take care when pipetting and mixing sample to minimize risk.

c) Kit/components have expired

Ensure that the kit is used before the expiry date. Ensure reconstituted standard d) Enzyme Substrate Solution is

Discard substrate if blue coloration exists. Ensure clean reagent reservoirs are

e) Mixing of plasma in QFN

After centrifugation, avoid pipetting up and down or mixing plasma by any

Low optical density readings for standards

a) Standard dilution error

Ensure dilutions of the kit standard are prepared correctly as per this instructions b) Pipetting error

Ensure pipets are calibrated and used according to manufacturer’s instructions.

c) Incubation temperature too

Incubation of ELISA should be performed at room temperature (17–27°C).

d) Incubation time too short

Incubate the plate with the conjugate, standards, and samples for 120 ± 5

e) Incorrect plate reader filter

Plate should be read at 450 nm with a reference filter between 620 and 650

Troubleshooting Guide

This troubleshooting guide may be helpful in solving any problems that may arise. For technical assistance and more information, please see our Technical Support Center at

www.qiagen.com/support (for contact information, visit www.qiagen.com)

Nonspecific color development

plate

wells

contaminated

tubes before harvesting

low

used

washing cycles may be required depending on the washer being used. A soak time of at least 5 seconds between cycles should be used.

and Conjugate 100x Concentrate are used within 3 months of the reconstitution date.

used.

means prior to harvesting. At all times, take care not to disturb material on the surface of the gel.

for use.

minutes. Incubate the Enzyme Substrate Solution on the plate for 30 minutes.

nm.

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Comments and suggestions

f) Reagents are too cold

All reagents, with the exception of the Conjugate 100x Concentrate, must be

. This takes

g) Kit/components have expired

Ensure that the kit is used before the expiry date. Ensure reconstituted standard

months of their

High background

a) Incomplete washing of the

Wash the plate at least 6 times with 400 µl/well of wash buffer. More than 6 b) Incubation temperature is too

Incubation of the ELISA should be performed at room temperature (17–27°C).

c) Kit/components have expired

Ensure that the kit is used before the expiry date. Ensure reconstituted Standard and Conjugate 100X Concentrate are used within three months of the

d) Enzyme Substrate Solution is

Discard substrate if blue coloration exists. Ensure clean reagent reservoirs are

Nonlinear standard curve and duplicate variability

a) Incomplete washing of the

Wash the plate at least 6 times with 400 µl/well of wash buffer. More than 6 b) Standard dilution error

Ensure dilutions of the standard are prepared correctly as per this package

c) Poor mixing

Mix reagents thoroughly by inversion or gentle vortexing prior to their addition

d) Inconsistent pipetting

Sample and standard addition should be performed in a continuous manner.

brought to room temperature prior to starting the ELISA approximately one hour.

and Conjugate 100x Concentrate are used within 3 reconstitution date.

plate

high

contaminated

plate

technique or interruption during ELISA setup

washing cycles may be required depending on the washer being used. A soak time of at least 5 seconds between cycles should be used.

reconstitution date.

used.

washing cycles may be required depending on the washer being used. A soak time of at least 5 seconds between cycles should be used.

insert.

to the plate.

All reagents should be prepared prior to starting the ELISA.

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Symbol

Description

Symbols

The following table describes the symbols that may appear on the labeling or in this document:

2 x 96

Sufficient for 2 x 96 sample preparations

Legal manufacturer

Batch code

Catalog number

Use by date

Temperature limitation

Consult instructions for use

Do not reuse

Keep away from sunlight

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Contact Information

For technical assistance and more information, please call toll-free 800-362-7737, see our Technical Support Center at www.qiagen.com/contact or contact one of the QIAGEN Technical Service Departments (see back cover or visit www.qiagen.com).

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

1. Equilibrate ELISA components, with the exception of

2. Reconstitute the kit standard to 8.0 IU/ml with distilled

3. Reconstitute freeze-dried Conjugate 100x Concentrate

4. Prepare working strength conjugate in Green Diluent

5. Add 50 µl test plasma samples and 50 µl standards

6. Incubate for 120 ± 5 minutes at room temperature.

7. Wash wells at least 6 times with 400 µl/well of wash

8. Add 100 µl Enzyme Substrate Solution to wells. Mix

Appendix A – Abbreviated Test Procedure

the Conjugate 100x Concentrate, to room temperature for at least 60 minutes.

or deionized water. Prepare standard curve dilutions.

with distilled or deionized water

and add 50 µl to all wells.

to the appropriate wells. Mix using shaker.

buffer.

using shaker.

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

9. Incubate for 30 minutes at room temperature.

10. Add 50 µl Enzyme Stopping Solution to all wells. Mix

11. Read results at 450 nm with a 620–650 nm reference

12. Analyze results.

using shaker.

filter.

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Product

Relative Products

Ordering Information

Contents Cat. no.

QuantiFERON ELISA

QuantiFERON SARS-CoV-2 Starter Set

QuantiFERON Extended Set Contains SARS-CoV-2 Ag3 tube 626215

QuantiFERON Control Set Contains Nil tube, and Mitogen Tube 626015

QuantiFERON Monitor Direct Contains Monitor Direct Tube 626315

For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen.com or can be requested from QIAGEN Technical Services or your local distributor.

Contains Microtiter Plate, Conjugate (100x), IFN Gamma Standard, Green Diluent, Wash Buffer, Enzyme Substrate Solution, and Enzyme Stopping Solution

Contains SARS-CoV-2 Ag1 tube and SARS-CoV-2 Ag2 tube

626410

626115

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Document Revision History

Date Changes

R1, November 2020 Initial release

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Limited License Agreement for QuantiF ERON® ELISA

Use of this product signifies the agreement of any purchaser or user of the product to the following terms:

1. The product may be used solely in accordance with the protocols provided with the product and this handbook and for use with components contained in the kit

only. QIAGEN grants no license under any of its intellectual property to use or incorporate the enclosed components of this kit with any components not included within this kit except as described in the protocols provided with the product, this handbook, and additional protocols available at www.qiagen.com. Some of these additional protocols have been provided by QIAGEN users for QIAGEN users. These protocols have not been thoroughly tested or optimized by QIAGEN. QIAGEN neither guarantees them nor warrants that they do not infringe the rights of third-parties.

2. Other than expressly stated licenses, QIAGEN makes no warranty that this kit and/or its use(s) do not infringe the rights of third-parties.

3. This kit and its components are licensed for one-time use and may not be reused, refurbished, or resold.

4. QIAGEN spe cifically disclaims any other licenses, expressed or implied other than those expressly stated.

5. The purchaser and user of the kit agree not to take or permit anyone else to take any steps that could lead to or facilitate any acts prohibited above. QIAGEN

may enforce the prohibitions of this Limited License Agreement in any Court, and shall recover all its investigative and Court costs, including attorney fees, in any action to enforce this Limited License Agreement or any of its intellectual property rights relating to the kit and/or its components.

For updated license terms, see www.qiagen.com.

Trademarks: QIAGEN®, Sample to Insight®, QFN®, QuantiFERON® (QIAGEN Group). Registered names, trademarks etc. used in this document, even when not specifically marked as such, are not to be considered unprotected by law.

For up-to-date licensing and product-specific disclaimers, see the QuantiFERON ELISA Package Insert

QuantiFERON ELISA Instructions for Use (Handbook) 11/2020

Ordering

www.qiagen.com/shop | Technical Support support.qiagen.com | Website www.qiagen.com

QIAGEN QuantiFERON Instruction Manual

Specifications and Main Features

Frequently Asked Questions

User Manual