QIAGEN EasiCollect Instruction Manual

Sample to Insight__

Quick-Start Protocol January 2021

Instructions for EasiCollect®

Warning: for research use only. Not for use in diagnostic procedures

EasiCollect is an all-in-one device for the collection and storage of buccal cells. Cells are
captured on the foam applicator by swabbing the inside of both cheeks and then transferred

®

to an Indicating FTA

FTA Cards are treated with a proprietary chemical mix that breaks open cells and releases DNA.
The same chemical mix then protects the DNA from biological or environmental damage. The
Indicating FTA Cards also contain a dye that turns white when colorle
to the card. This provides an easy method for sample location.

Further information

 Safety Data Sheets: www.qiagen.com/safety

 Technical assistance: support.qiagen.com

Card held within the device.

ss liquids are applied

Storage

Store product at room temperature (15–25°C). After applying samples, allow the Indicating
FTA Card to dry, and then store in a multibarrier pouch (WB100036) at room temperature in
a dry environment. When stored correctly, the Indicating FTA Cards are good until the
expiration date printed on the kit box lid. Dried sample cards can be stored for long periods
of time in a multibarrier pouch with a desiccant packet (WB100003).

2

Instructions for EasiCollect 01/2021

Hinge

2-Position Stem Lock

Foam Head

Sample Area

Head Tab

FTA Card

Notes before starting

 Always wear gloves when handling biological samples, and avoid touching the surface

of the collection device to prevent contamination.

 Sample collection may be improved if subjects avoid eating, drinking, or smoking 30 min

prior to sample collection.

Figure 1. Key features of the EasiCollect device.

Procedure

Preparation of the device

1. Do not open packages before use. When collecting samples, always wear gloves.

2. Open the protective sleeve at the FTA Card end. Slide out the EasiCollect device, leaving
the foam-tip in the packaging.

3. Record identification information on the back of the FTA Card and then remove
completely from the packaging sleeve.

Instructions for EasiCollect 01/2021

3

Buccal cell collection

4.

Hold the EasiCollect as shown (
moderate pressure, run it along the gum-line and under the tongue. Once the foam

ad is wet with saliva, rub the inside of each cheek for 15 s. Ensure that you feel/see the

he
cheek protrude slightly during the collection. It is extremely important to ensure that the
foam head is coated with saliva during this step to enable good sample transfer.

5. Remove the foam head from the mouth.

6. Carefully remove the plastic protective film from the FTA Card holder (Figure 2) to expose
the FTA Card.

Figure 2. Sample uptake using the EasiCollect device.

Figure 2

). Place the foam head in the mouth, and using

Sample transfer

7. Fold the device at the hinge (1), and press the foam head onto the FTA Card (Figure 3).

8. Ensure that the foam head is held in place with the stem lock in the lowest position (2),
and the foam head under the head tab (3).

9. Leave the foam head in contact with the FTA Card for 10 s.