ProteinSimple NanoPro 1000 User Manual
NanoPro 1000 System User Guide
page 1
Copyright © 2013 ProteinSimple. All rights reserved.
ProteinSimple
3040 Oakmead Village Drive
Santa Clara, CA 95051
Toll-free: (888) 607-9692
Tel: (408) 510-5500
Fax: (408) 510-5599
email: support@proteinsimple.com
web: www.proteinsimple.com
NanoPro 1000 System User Guide
P/N 001-473
Revision 5, June 2013
For research use only. Not for use in diagnostic procedures
Patents and Trademarks
Automatic Image Capture (AIC) and digital ProteomeChip technology are covered by U.S. Patent Nos
6,995,901, 6,909,459, 6,853,454, 6,271,042, 7,166,202, and other issued and pending patents in the U.S. and
other countries. ProteinSimple, the ProteinSimple logo, the Alpha Innotech logo, Protein Forest, the Protein
Forest logo, the AIC logo, AlphaCal, AlphaImager, AlphaPart11View, AlphaQuant, AlphaSnap, AlphaSpec,
AlphaUV, AlphaView, ChemiGlow, Chromalight, dPC, digital ProteomeChip, FluorChem, MSRAT, MultiImage,
NanoPro, the intertwined helix design, iWB, Peggy, ProteomeChip, red, Sally, Simon, Simple Western, Spectra
Plex, Xpedition, XplorBright, and XplorUV are trademarks or registered trademarks of ProteinSimple. Other
marks appearing in these materials are marks of their respective owners.
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Table of Contents
page i
Chapter 1:
General Information . . . . . . . . . . . . . . . . . . . . . . . . 1
Welcome . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Simple Western Charge Separation Assays . . . . . . . . . . . 2
Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
User Attention Notifications. . . . . . . . . . . . . . . . . . . . . . 3
Chemical Hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Chemical Waste Hazards . . . . . . . . . . . . . . . . . . . . . . . . 4
Waste Production and Disposal. . . . . . . . . . . . . . . . . . 5
Material Safety Data Sheets. . . . . . . . . . . . . . . . . . . . . . 6
Instrument Safety Labels . . . . . . . . . . . . . . . . . . . . . . . . . 6
Customer Service and Technical Support . . . . . . . . . . . . . 8
Legal Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
NanoPro 1000 System Disclaimer of Warranty. . . 9
Compass Software and Authorization Server
License Agreement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Chapter 2:
Lab Requirements. . . . . . . . . . . . . . . . . . . . . . . . . . 13
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14
Space Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
Environmental Requirements. . . . . . . . . . . . . . . . . . . . . . . .16
Software and Computer Requirements . . . . . . . . . . . . . . 17
General Guidelines and Information. . . . . . . . . . . . . . . . .17
System Operation: Notification of Intended Use. 17
Lifting and Moving the System: Use Proper Lifting
Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Chapter 3:
NanoPro 1000 System . . . . . . . . . . . . . . . . . . . . . 19
Instrument Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
External Components . . . . . . . . . . . . . . . . . . . . . . . . . . .20
Internal Components . . . . . . . . . . . . . . . . . . . . . . . . . . .23
Auxiliary Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Removing and Installing Bottles . . . . . . . . . . . . . . . . 36
Connection Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
Compliance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
Safety Guidelines. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Door Interlock. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40
System Trays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40
Auxiliary Module Reservoirs . . . . . . . . . . . . . . . . . . . . .40
Physical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
Chapter 4:
Operating the NanoPro 1000 System. . . . 43
Power Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Starting a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
Step 1 - Get Ready . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Step 2 - Start the Run . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
Step 3 - Post-Run Procedures . . . . . . . . . . . . . . . . . . . .51
Stopping a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Waste and Water Bottles. . . . . . . . . . . . . . . . . . . . . . . . . . . . .52
NanoPro 1000 System User Guide
page ii
Controlling the NanoPro 1000 System . . . . . . . . . . . . . . .53
Starting a New Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .54
Cleaning After a Run Error . . . . . . . . . . . . . . . . . . . . . .56
Self Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57
Viewing and Changing System Properties . . . . . .58
Viewing Error and Test Logs. . . . . . . . . . . . . . . . . . . . . 58
System Status Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Shutdown . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Chapter 5:
Maintenance and Troubleshooting . . . . . . 61
Software Updates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
Daily . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Monthly. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
Yearly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Instrument Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Manifold Flush . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
System Decontamination. . . . . . . . . . . . . . . . . . . . . . . 66
Preparation for Storage or Shipment . . . . . . . . . . . . . . . . 68
Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .68
Shipment Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
NanoPro 1000 System User Guide
Chapter 1:
General Information
Chapter Overview
•Welcome
page 1
• Simple Western Charge Separation Assays
•Safety
• Customer Service and Technical Support
•Legal Notices
NanoPro 1000 System User Guide
page 2 Chapter 1: General Information
Welcome
Congratulations on your purchase of the NanoPro™ 1000 system. ProteinSimple welcomes you as a new
user. This user guide will cover important safety and instrument information, installation requirements, main
tenance and operating instructions.
Simple Western Charge Separation Assays
Simple Western charge separation assays take place in a capillary. The system automates all steps of the process for you including sample loading, protein separation, immunoprobing, washing, detection and data
analysis. Up to 12 samples can be analyzed simultaneously in a single cycle and up to 8 cycles (96 samples)
can be processed in a single run.
Simple Western charge assays let you do in-depth characterization of proteins. Because proteins are separated by their charge or pI, extremely small isoelectric point differences are detected - letting you see discreet changes in protein isoforms.
Native protein lysate samples and assay reagents are
prepared, loaded into an assay plate and placed in the
NanoPro 1000 system.
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The NanoPro 1000 system loads samples into the capillary automatically. Proteins and ampholytes migrate
through a separation matrix, are separated by charge
and resolve according to their expected pI values. The
separated proteins are then immobilized to the capil
lary wall via a proprietary UV capture method. Target
proteins are immunoprobed using a primary antibody
and detected using an HRP-conjugated secondary
antibody and chemiluminescent substrate. The result
ing chemiluminescent signal is detected and quantitated. Analysis is automatically performed and
quantitative results, including pI value and signal
intensity, are presented in Compass software.
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NanoPro 1000 System User Guide
Safety page 3
Safety
User Attention Notifications
Several user attention phrases are used throughout this manual. Each phrase should draw the following
level of attention from the user:
NOTE Points out useful information.
IMPORTANT Indicates information necessary for proper instrument operation.
CAUTION Cautions users regarding potentially hazardous situations in regard to user injury or dam-
age to the instrument if the information is not heeded.
!WARNING! Warns users that serious physical injury can result if warning precautions are not heeded.
Chemical Hazards
!WARNING! CHEMICAL HAZARD
Some chemicals used can be potentially hazardous, and can cause injury or illness.
• Read and understand the Material Safety Data Sheets (MSDSs) provided by the chemical manufacturer before you store, handle, or work with any chemicals or hazardous materials.
• Minimize contact with and inhalation of chemicals. Wear appropriate personal protective equipment
when handling chemicals (e.g., safety glasses, gloves, or clothing). For additional safety guidelines,
consult the MSDS.
• Do not leave chemical containers open.
• Check regularly for chemical leaks or spills. If a leak or spill occurs, follow the manufacturer's cleanup
procedures as recommended on the MSDS.
• Comply with all local, state/provincial, or national laws and regulations related to chemical storage,
handling, and disposal.
NanoPro 1000 System User Guide
page 4 Chapter 1: General Information
Chemical Waste Hazards
!WARNING! BIOHAZARD
Samples and waste bottle contents should be handled by procedures recommended in
the CDC/NIH manual: Biosafety in Microbiological and Biomedical Laboratories (BMBL).
The manual is available from the U.S. Government Printing Office or online at
http://www.cdc.gov/biosafety/publications/bmbl5/.
Depending on the samples used, waste bottle contents may constitute a biohazard. Use
precaution when emptying the waste bottle. Dispose of waste bottle contents in accordance with good laboratory practices and local, state/provincial, or national environmental and health regulations.
• Read and understand the Material Safety Data Sheets (MSDSs) provided by the manufacturers of the
chemicals in the waste container before you store, handle, or dispose of chemical waste.
• Minimize contact with chemical waste. Wear appropriate personal protective equipment when handling chemicals (e.g., safety glasses, gloves, or clothing).
• Use precaution when emptying the waste bottle.
• Dispose of waste bottle contents in accordance with good laboratory practices and local, state/provincial, or national environmental and health regulations.
NanoPro 1000 System User Guide
Safety page 5
Waste Production and Disposal
It is the responsibility of the user to properly dispose of waste. The aqueous bulk waste stream is approximately pH 8. For reference, ProteinSimple disposes of waste under the non-RCRA Hazardous Waste Liquid
code 141 (buffer containing sodium chloride surfactants). A typical waste profile for one cycle (12 capillaries)
is as follows:
NOTE: Volumes should be adjusted accordingly to account for running of additional cycles.
.
Waste Origin Volume
Trough Reagents for 1 cycle
Anolyte (10 mM) 1.0 mL
Catholyte (100 mM) 1.0 mL
Wash Buffer 3.0 mL
Water (for trough and manifold cleaning) 80.0 mL
Total waste per cycle 85.0 mL
Assay Plate Reagents*
Luminol/Peroxide Solution 0.025 mL
Antibodies in Antibody Diluent 0.075 mL
Example Cell Lysate Sample
Cell lysate
Sample Diluent
Premix
Protease inhibitors
Phosphatase inhibitors
Labeled peptides 0.036 mL
*Assumes 1 row of sample and 1 row of antibody.
NanoPro 1000 System User Guide
page 6 Chapter 1: General Information
Material Safety Data Sheets
Some chemicals used with the NanoPro 1000 system may be listed as hazardous. Warnings are displayed on
the labels of all chemicals when hazards exist.
MSDSs provide users with safety information needed to store, handle, transport and dispose of the chemicals safely. ProteinSimple recommends updating laboratory MSDS records periodically.
Material Safety Data Sheets for ProteinSimple reagents are available online at www.proteinsimple.com/literature or by calling (888) 607-9692. Otherwise, call the chemical manufacturer directly or visit their web site.
Instrument Safety Labels
The following safety labels are located on the NanoPro 1000 system. Each label will display a safety alert symbol indicating a potential safety hazard.
Symbol Description
Risk of Electric Shock.
Refer to the NanoPro 1000 System User Guide before proceeding.
Danger of hazardous waste. Use caution in these areas. This warning only applies if using
hazardous material. The NanoPro 1000 system reagents are not considered hazardous
waste. If you are using hazardous materials, please contact your field service representa
tive to place labels in the appropriate locations.
!WARNING!
If the unit is not used as specified by ProteinSimple, the overall safety will be impaired.
!WARNING!
If the unit is damaged and does not function properly, stop the unit safely and contact ProteinSimple Technical Support immediately.
!WARNING!
No user replaceable/serviceable parts except for the ionizer fan.
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NanoPro 1000 System User Guide
Safety page 7
Sharps Hazard
!WARNING! SHARPS HAZARD
Capillaries may present a potential sharps hazard. Dispose of used capillaries in biomedical waste
sharps containers.
Pinch Hazard
!WARNING! PINCH HAZARD
Do not remove the transparent front window on the NanoPro 1000 system unless instructed to do
so by ProteinSimple Technical Support personnel. The window protects users from a potential
pinch hazard that could be caused by the moving robot and gripper assembly.
Door Interlock
A door interlock is engaged when a run is started to prevent users from opening the doors during a run. This
eliminates any dangerous user interactions with the robot as well as exposure to high voltage and UV light.
A yellow indicator light indicates when the instrument is running and the interlock is engaged.
When the system status is READY and the doors are open, the robot's XZ stage motors, high-voltage power
supply and UV power supply are disabled.
!WARNING!
Do not override the door interlock. The interlock protects users from exposure to UV light and a
potential pinch hazard that could be caused by the moving system tray.
System Tray
CAUTION
Do not attempt to open or close the tray manually. Instead, use the Open Tray command from the
Instrument menu in Compass software.
NanoPro 1000 System User Guide
page 8 Chapter 1: General Information
Prior to System Operation
Ensure that all users of the NanoPro 1000 system have:
• Received instruction in general safety practices for laboratories.
• Received instruction in specific safety practices for the instrument.
• Received instruction on handling of biohazards if biohazardous materials are to be used on the system.
• Read and understood all related MSDSs.
CAUTION
Avoid using the NanoPro 1000 system in a manner not specified by ProteinSimple. While the system
has been designed to protect the user, this protection may be impaired if the instrument is used
improperly.
Customer Service and Technical Support
Te le ph on e
(408) 510-5500
(888) 607-9692 (toll free)
Fax
(408) 510-5599
E-mail
support@proteinsimple.com
Web
www.proteinsimple.com
Address
ProteinSimple
3040 Oakmead Village Drive
Santa Clara, CA 95051
USA
NanoPro 1000 System User Guide
Legal Notices page 9
Legal Notices
NOTE: Read the Legal Notices carefully before using the NanoPro 1000 system.
NanoPro 1000 System Disclaimer of Warranty
EXCEPT AS EXPRESSLY PROVIDED IN ANY ProteinSimple SOFTWARE LICENSE AGREEMENT OR QUOTATION, THE PRODUCTS SOLD AND SERVICES PROVIDED BY ProteinSimple ARE PROVIDED ON AN “AS IS” AND “AS AVAILABLE” BASIS WITHOUT WARRANTY OF ANY KIND. ProteinSimple
AND ITS SUP PLIERS DO NOT WAR RANT TH E SECURIT Y, PRI VACY, OR ACCU RACY OF ANY DATA PROVID ED VIA T HE PRODUC TS OR S ERVIC ES, AND
YOU AGREE THAT THE USE OF ANY SUCH DATA BY YOU IS AT YOUR SOLE RISK. TO THE MAXIMUM EXTENT ALLOWED UNDER APPLICABLE LAW,
ProteinSimple AND ITS SUPPLIERS DISCLAIM ANY AND ALL WARRANTIES, WHETHER EXPRESS, IMPLIED OR STATUTORY, INCLUDING, WITHOUT
LIMITATION, ANY WARRANTY OF MERCHANTABILITY, TITLE, NON-INFRINGEMENT, OR FITNESS FOR A PARTICULAR PURPOSE.
Compass Software and Authorization Server License Agreement
IMPORTANT - PLEASE READ CAREFULLY THE TERMS OF THIS COMPASS SOFTWARE AND AUTHORIZATION SERVER LICENSE AGREEMENT
(“AGREEMENT”). BY CLICKING ON THE “I AGREE” BUTTON, (1) YOU ACKNOWLEDGE THAT YOU HAVE READ, UNDERSTAND, AND AGREE TO BE
BOUND BY THIS AGREEMENT AND (2) YOU REPRESENT THAT YOU HAVE THE AUTHORITY TO ENTER INTO THIS AGREEMENT, PERSONALLY OR IF
YOU HAVE NAMED A COMPANY AS CUSTOMER, ON BEHALF OF THAT COMPANY (YOU OR ANY SUCH COMPANY, THE “CUSTOMER”), AND TO
BIND THE CUSTOMER TO THE TERMS OF THIS AGREEMENT. IF YOU DO NOT AGREE TO ALL TERMS AND CONDITIONS OF THIS AGREEMENT, OR
IF YOU DO NOT HAVE SUCH AUTHO RITY, YOU SHOULD CL ICK ON THE “CANCEL” BUTTON TO DISCONTINUE THE DOWNLOAD OF THE LICENSED
SOFTWARE.
1. Definitions
1.1 “Authorized Use Parameters” means the following usage restrictions, which restrict the operation of the Licensed Software to a
particular set of conditions: Customer shall (a) limit simultaneous use of the Licensed Software to a maximum of ten (10) Authorized
Users; and (b) use the Licensed Software only in connection with the accompanying System purchased by Customer pursuant to the
System Quotation and located at the Site.
1.2 “Authorized User” means one (1) User who initiates the execution of the Licensed Software and/or interacts with or directs the
Licensed Software in the performance of its functions. Multiple Authorized Users may work simultaneously with one installation of the
Licensed Software, as on a server, or they may each have their own installation on single-user machines, or a mix of these, provided
that in all cases the total number of simultaneous Users does not exceed the applicable Authorized Use Parameters.
1.3 “Company” means ProteinSimple.
1.4 “Documentation” means Company's then-current manuals, guides, and on-line help pages, if any, applicable to the Licensed
Software and made generally available by Company to its customers.
1.5 “Enterprise” means those organizations that have Internet addresses located at top level and second-level domain names set forth in
the System Quotation.
1.6 “Error” means a reproducible error in the Licensed Software that prevents such Licensed Software from operating substantially in
accordance with its Documentation.
1.7 “Executable Code” means the fully compiled binary version of Licensed Software that can be executed by a computer and used by
an end user without further compilation.
1.8 “Intellectual Property Rights” means all copyrights, trade secrets, patents, patent applications, moral rights, contract rights, and
other proprietary rights, but specifically excluding any trademarks or service marks.
1.9 “Licensed Software” means the Compass software program in Executable Code form, and any Updates that Company makes
available to Customer in accordance with this Agreement.
NanoPro 1000 System User Guide
page 10 Chapter 1: General Information
1.10 “Site” means the facility or campus set forth in the System Quotation.
1.11 “System” means the proprietary NP1000, NP100, Simon, Sally, or Peggy protein analysis system or any future model or successor
thereto that is provided to Customer by Company pursuant to a separate agreement between the parties (the “System Quotation”).
1.12 “Update” means those releases of the Licensed Software that Company provides to customers to correct Errors, fix bugs, or create
minor improvements, incremental features, or enhancements of existing features which Company designates by a change in the
number to the right of the first or second decimal point. Updates do not include those releases of the Licensed Software that provide
substantial new features or additional functionality which Company designates by a change in the number to the left of the first
decimal point.
1.13 “User” means any individual that has an e-mail address within the Enterprise.
2. License and Restrictions
2.1 License Grant. Subject to the terms and conditions of this Agreement and the payment of the required fees set forth in the System
Quotation, Company grants to Customer a nontransferable, nonexclusive, royalty-free, revocable, worldwide license (without the right
to sublicense) to (a) install the Licensed Software on any computer located at any Site; (b) use, execute, and display the Licensed
Software, in Executable Code form only; and (c) copy the Licensed Software and Documentation, solely as necessary to support
Authorized Users; in each of the foregoing, solely in accordance with the Documentation and the Authorized Use Parameters.
Customer agrees that it will comply with the Authorized Use Parameters.
2.2 License Restrictions. Customer acknowledges that the Licensed Software and its structure and organization constitute valuable
trade secrets of Company. Accordingly, the license granted in this Agreement is subject to the following restrictions: Customer and its
Authorized Users (a) may not reverse engineer, disassemble, decompile, or otherwise attempt to derive the source code of Licensed
Software; (b) may not modify, adapt, alter, translate, or create derivative works from the Licensed Software; (c) may not merge the
Licensed Software with other software; (d) may not use the Licensed Software in any service bureau or time-sharing arrangement,
license, sell, rent, lease, transfer, assign, distribute, host, outsource, disclose, or otherwise commercially exploit or make the Licensed
Software or Documentation available to any third party; (e) shall only make that number of exact copies of the Licensed Software and
Documentation as delivered by Company that are necessary to support Customer's use of the Licensed Software in accordance with
this Agreement; (f) shall include any titles, trademarks, and copyright and restricted rights notices that are included on or in the
Licensed Software as delivered by Company on and in any copies of the Licensed Software that it makes; and (g) shall ensure that
Customer's use of the Licensed Software does not exceed the scope of the license that Customer has purchased pursuant to this
Agreement.
2.3 Open Source Software. Certain items of independent, third-party code may be included in the Licensed Software that are subject to
open source licenses (“Open Source Software”). Such Open Source Software is licensed under the terms of the license that
accompanies such Open Source Software. Nothing in this Agreement limits Customer's rights under, or grants Customer rights that
supersede, the terms and conditions of any applicable end user license for such Open Source Software. In particular, nothing in this
Agreement restricts Customer's right to copy, modify, and distribute such Open Source Software that is subject to the terms of such
open source licenses.
2.4 Ownership. Company reserves all rights not expressly granted to Customer in this Agreement. Without limiting the generality of the
foregoing, Customer acknowledges and agrees that, except as expressly set forth in this Agreement, Company and its suppliers retain
all Intellectual Property Rights, title and interest in and to the Licensed Software and Documentation.
3. Support and Maintenance Services
3.1 Services. Subject to Customer's payment of the Services fees, as set forth in the System Quotation, and to the terms and conditions
herein, Company will use commercially reasonable efforts to provide to Customer the following support and maintenance services
(the “Services”) for the Licensed Software: (a) Company will answer technical questions concerning functions and features of the
Licensed Software; (b) Company will provide Error verification, analysis and corrective efforts for the Licensed Software; and (c)
Company will provide, without charge, Updates of the software released during the term of this Agreement. Customer will be
responsible for providing, in a manner consistent with good industry practice, all Services to Users. Customer acknowledges that
Company may not be able to correct all reported Errors. Any Update of the Licensed Software will be deemed part of the Licensed
Software and Customer will use such Updates in accordance with the requirements and obligations in this Agreement.
3.2 Service Conditions. Company's obligation to provide the Services is conditioned on Customer: (a) notifying Company of any Error
within a reasonable period of time; (b) providing Company all information relating to the Error; (c) providing access to the Licensed
Software and Customer's facility where the Licensed Software is located and informing Company of any potential hazards which may
be encountered while servicing the Licensed Software. Customer may contact Company via telephone at 1-888-607-9692 or e-mail at
NanoPro 1000 System User Guide
Legal Notices page 11
support@proteinsimple.com during the hours of 8 a.m. (Pacific Time) and 5 p.m. (Pacific Time) Monday through Friday, excluding
holidays, to report any Error. A list of standard holidays will be provided to Customer upon request. Company shall have the right to
determine in its sole discretion what corrective action Company will perform to support the Licensed Software. Company may
subcontract the Services to a third party contractor provided that Company will be responsible for the third party contractor's
compliance with this Agreement.
3.3 Service Exclusions. Company will not be obligated to provide the Services if (a) Company determines that an Error is caused by
malfunction of any hardware (other than malfunction of the System) or third party software used with the Licensed Software; or (b)
Customer has failed to incorporate the latest Update previously released to Customer.
4. Warr anty
4.1 Licensed Software Warranty. Company warrants that the Licensed Software, as properly installed, and under normal use, will
perform substantially in accordance with its Documentation during the Warranty Period. The “Warranty Period” for the Licensed
Software begins on date Customer downloads the Licensed Software and ends twelve (12) months thereafter.
4.2 Remedy. If Customer notifies Company in writing during the Warranty Period of an Error, Company will, at its expense and as its sole
obligation for any breach of the foregoing warranty, use commercially reasonable efforts to correct the Error or replace the Licensed
Software. Any Error correction or replacement of the Licensed Software will not extend the original Warranty Period. The warranty and
the remedies provided above will not apply to the Licensed Software if (a) Company determines that an Error is caused by accident,
abuse, misuse, negligence, fire, earthquake, flood, other force majeure event, failure of electrical power, the use of unauthorized
products, or unauthorized repairs or modifications; (b) Company determines that an Error is caused during or as a result of delivery; (c)
a problem arises from or is based on Company's compliance with Customer's specifications; or (d) Company determines that an Error
is caused by malfunction of any hardware (other than malfunction of the System) or third party software used with the Licensed
Software.
4.3 Disclaimer. THE WARRANTIES ABOVE ARE EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER EXPRESS, IMPLIED OR
STATUTORY, INCLUDING WITHOUT LIMITATION THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR
PURPOSE, TITLE, AND NONINFRINGEMENT.
5. Limitation of Liability. NEITHER COMPANY NOR ITS SUPPLIERS SHALL BE RESPONSIBLE OR LIABLE WITH RESPECT TO ANY SUBJECT MATTER
OF THIS AGREEMENT OR TERMS OR CONDITIONS RELATED THERETO UNDER ANY CONTRACT, NEGLIGENCE, STRICT LIABILITY OR OTHER
THEORY (A) FOR LOSS OR INACCURACY OF DATA, LOSS OF PROFITS OR COST OF PROCUREMENT OF SUBSTITUTE GOODS, SERVICES OR
TECHNOLOGY, OR (B) FOR ANY INDIRECT, INCIDENTAL OR CONSEQUENTIAL DAMAGES INCLUDING, BUT NOT LIMITED TO LOSS OF
REVENUES AND LOSS OF PROFITS. COMPANY'S AGGREGATE CUMULATIVE LIABILITY HEREUNDER SHALL NOT EXCEED THE GREATER OF FIVE
HUNDRED DOLLARS ($500.00).
6. Term and Termination
6.1 Term of Agreement. The Agreement is effective on the date Customer downloads the Licensed Software and shall remain in effect
until terminated by either party as provided in this section.
6.2 Termination For Material Breach. Either party may terminate this Agreement upon written notice if the other party materially
breaches this Agreement and fails to cure such breach within thirty (30) calendar days following receipt of written notice from the
other party specifying the breach in detail. Notwithstanding the foregoing, Company may immediately terminate this Agreement and
all licenses granted hereunder if Customer breaches Section 2 (License and Restrictions) hereof or upon termination of the System
Quotation. The foregoing rights of termination are in addition to any other rights and remedies provided in this Agreement or by law.
6.3 Effect of Termination. Upon termination of this Agreement (or termination or expiration of any license granted hereunder), all rights
of Customer to use the Licensed Software and Documentation will cease and (a) all license rights granted under this Agreement will
immediately terminate and Customer shall promptly stop all use of the Licensed Software and Documentation; (b) all Services will
terminate immediately; (c) Customer shall promptly erase all copies of the Licensed Software from Customer's computers, and destroy
all copies of the Licensed Software and Documentation on tangible media in Customer's possession or control or return such copies
to Company; and (d) upon request by Company, Customer shall certify in writing to Company that it has returned or destroyed such
Licensed Software and Documentation. The parties' rights and obligations under Sections 1 (Definitions), 2.4 (Ownership), 4.3
(Disclaimer), 5 (Limitation of Liability), 6 (Term and Termination), and 7 (General) shall survive termination of this Agreement.
7. General
NanoPro 1000 System User Guide
page 12 Chapter 1: General Information
7.1 Assignment. This Agreement and Customer's rights hereunder may not be assigned to any third party by Customer except with the
prior written approval of Company. Any attempted assignment of this Agreement or any rights or obligations hereunder will be null
and void.
7.2 Governing Law. This Agreement is made in, governed by, and shall be construed in accordance with the laws of the State of
California, without regard to any conflicts of law principles that would result in application of laws of any other jurisdiction. The United
Nations Convention on Contracts for the International Sale of Goods does not apply to this contract. Any legal action or other legal
proceeding relating to this contract or the enforcement of any provision of this contract must be brought in any state or federal court
located in Santa Clara County, California. Customer and Company expressly and irrevocably consents and submits to the jurisdiction of
such courts.
7.3 Injunctive Relief. Customer acknowledges that the Licensed Software contains valuable trade secrets and proprietary information of
Company, that any actual or threatened breach of this Agreement will cause harm to Company for which monetary damages would
be an inadequate remedy, and that injunctive relief is an appropriate remedy for such breach.
7.4 Modifications. Company reserves the right to change the terms and conditions of this Agreement or its policies relating to the
Licensed Software at any time. Company will notify Customer of any material changes to this Agreement by sending Customer an email to the last e-mail address Customer provided to Company or by prominently posting notice of the changes on Company's
website. Any material changes to this Agreement will be effective upon the earlier of thirty (30) calendar days following Company's
dispatch of an e-mail notice to Customer or thirty (30) calendar days following Company's posting of notice of the changes on
Company's website. These changes will be effective immediately for new users of our Licensed Software. Please note that at all times
Customer is responsible for providing Company with its most current e-mail address. In the event that the last e-mail address that
Customer has provided Company is not valid, or for any reason Company is not capable of delivering to Customer the notice
described above, Company's dispatch of the e-mail containing such notice will nonetheless constitute effective notice of the changes
described in the notice. If Customer does not agree with the changes to this Agreement, Customer must notify Company prior to the
effective date of the changes that Customer wishes to terminate its license to the Licensed Software. Continued use of the Licensed
Software, following notice of such changes, shall indicate Customer's acknowledgement of such changes and agreement to be bound
by the terms and conditions of such changes.
7.5 Severability. In the event any provision of this Agreement is held to be invalid or unenforceable, the remaining provisions of this
Agreement will remain in full force.
7.6 Waiv er. The waiver by either party of any default or breach of this Agreement shall not constitute a waiver of any other or subsequent
default or breach.
7.7 Export. Customer agrees not to export, reexport, or transfer, directly or indirectly, any U.S. technical data acquired from Company, or
any products utilizing such data, in violation of the United States export laws or regulations.
7.8 Force Majeure. Company shall not be liable, directly or indirectly, for any delay or failure in performance of any obligation under this
Agreement, including any delivery obligation, where such delay or failure arises or results from a cause beyond Company's reasonable
control, or beyond the reasonable control of Company's suppliers or contractors, including, but not limited to strike, boycott or other
labor disputes, embargo, governmental regulation, inability or delay in obtaining materials, acts of God, war, earthquake, fire, or flood.
In the event of such force majeure, the time for delivery or other performance will be extended for a period equal to the duration of
the delay caused thereby, provided that Company notifies Customer of the nature and duration of such force majeure event.
7.9 Entire Agreement; Notice. This Agreement constitutes the complete agreement between the parties and supersedes all prior or
contemporaneous agreements or representations, written or oral, concerning the subject matter of this Agreement. Except as
otherwise expressly provided in this Agreement, any modifications of this Agreement must be in writing and agreed to by both
parties. Company may provide any notice to Customer by e-mail. Customer may provide notice to Company by sending an e-mail to
info@proteinsimple.com or a letter by United States mail to ProteinSimple, 3040 Oakmead Village Drive, Santa Clara, CA 95051, or to
such other address as Company may specify in writing by posting the new address on the Company website.
7.10 Relationship of the Parties. The parties are acting hereunder as independent contractors and not as partners, agents, fiduciaries, or
joint venturers. Neither party has the power or authority represent, act for, bind, or otherwise create or assume any obligation on
behalf of the other party.
NanoPro 1000 System User Guide
Chapter 2:
Lab Requirements
Chapter Overview
•Introduction
page 13
• Space Requirements
• Electrical Requirements
• Environmental Requirements
• Software and Computer Requirements
• General Guidelines and Information
NanoPro 1000 System User Guide
page 14 Chapter 2: Lab Requirements
Introduction
This chapter will help you prepare the lab for the NanoPro 1000 system. Please complete the space, electrical, vacuum and environmental requirements prior to scheduling your installation.
NOTE: Please wait for an authorized ProteinSimple representative to unpack and install the NanoPro 1000
system. Do not attempt to do this as improper handling of the instrument and computer can result in personal injury or damage to the system.
Space Requirements
Please provide adequate space to accommodate the system and computer, with sufficient clearance for
heat ventilation and service accessibility. The NanoPro 1000 system is a bench-top unit that requires a sturdy
lab bench or table that can support 200 lbs (91 kg).
IMPORTANT
The NanoPro 1000 system must be on a stable surface and level at all times for proper operation.
Ensure the lab bench or table does not shift or wobble under heavy weight. Additionally, anti-vibration tables may not be used as they can alter the instrument level during operation.
NanoPro 1000 System User Guide
Space Requirements page 15
System dimensions are as follows:
Dimension Meters Feet
NanoPro 1000 System
Width 0.94 3.08
Width with doors open 1.27 4.17
Depth 0.61 2.00
Height 0.84 2.76
Auxiliary Module
Width 0.33 1.08
Depth 0.30 1.00
Height 0.51 1.67
Tubing between Auxiliary
3.00 9.84
Module and Instrument
Computer Workstation
Width 0.66 2.17
Depth 0.76 2.49
Height 0.41 1.35
Table 2-1: NanoPro 1000 system dimensions.
Dimension Meters Feet
Width 2.13 6.98
Depth 0.91 2.99
Height 1.00 3.28
Table 2-2: Recommended space requirements for the NanoPro 1000 system.
NanoPro 1000 System User Guide
page 16 Chapter 2: Lab Requirements
Electrical Requirements
The NanoPro 1000 system requires a dedicated, grounded circuit capable of delivering the appropriate current and voltage for your country. The power requirements for select countries are listed below:
Region Volts (AC) Frequency (Hz) Amps
US and Canada 120 60 5.5
Europe 240 50 2.7
Japan 100 50/60 6.5
Table 2-3: Power requirements.
In addition to the requirements listed above, ProteinSimple requires the grounded circuits terminate at the
receptacles, and receptacles must be located within 10 ft (3 m) of the instrument.
Environmental Requirements
Please provide appropriate heating and cooling to maintain a constant laboratory temperature. For optimal
performance, the lab environment must meet the following criteria:
Requirement Specification
Operating temperature range 18 - 24 °C (64 - 75 °F)
Operating humidity range 20-60% relative, non-condensing
Table 2-4: Environmental requirements.
NanoPro 1000 System User Guide
Software and Computer Requirements page 17
Software and Computer Requirements
The NanoPro 1000 system includes a computer to run the instrument and analyze resulting data using Compass software. The software is preinstalled. A CD containing Compass software is also provided with the
NanoPro 1000 system. For post-run data analysis, Compass software can also be installed on a separate
workstation, such as a user’s desktop computer. This computer must meet the minimum requirements listed
in the table below in order to run Compass software and process data.
Component Minimum Recommended
Operating System Windows XP, Windows 7 or Vista
Processor Core 2 Duo
Memory 2 GB
Free Disk Space 10 GB
Table 2-5: Computer requirements.
General Guidelines and Information
System Operation: Notification of Intended Use
NOTE: The NanoPro 1000 system is for research use only. Not for use in diagnostic procedures.
Lifting and Moving the System: Use Proper Lifting Precautions
IMPORTANT
Use caution when lifting or moving the NanoPro 1000 system. The system weight is 82 kg (180 lbs).
Two or more people are required to lift the system onto the laboratory bench. Proper lifting safety
precautions must be taken.
NanoPro 1000 System User Guide
page 18 Chapter 2: Lab Requirements
NanoPro 1000 System User Guide
Chapter 3:
NanoPro 1000 System
Chapter Overview
• Instrument Overview
page 19
• Auxiliary Module
•Compliance
• Safety Guidelines
• Physical Specifications
NanoPro 1000 System User Guide
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