ProteinSimple Maurice CE-SDS Application Manual

Maurice® CE-SDS Application Guide

Maurice CE-SDS Application Guide

Introduction

The CE-SDS Application Kit is a one-stop shop for all
your size application development needs! It includes
the CE-SDS cartridges, all sample preparation reagents
and consumables that’ll let you tackle any monoclonal
antibody. This Application Guide will help you every step
of the way.

If you add the IgG Standard, which is produced with a
known quantity of non-glycosylated heavy chain, you can
test resolution and quantitation suitability to make sure
your Maurice is ready to go.

CE-SDS Size Application Kit
contents (P/N PS-MAK02-S)

INCLUDES QTY/KIT

Maurice CE-SDS Cartridges 2

Maurice CE-SDS Cartridge Cleaning Vials 2

Maurice clear screw caps for sample and reagent vials 100

Maurice glass reagent vials, 2 mL 100

Maurice CE-SDS orange pressure caps 12

Maurice 96-well plates 10

Maurice CE-SDS Separation Matrix 15 mL

Maurice CE-SDS Running Buer – Top 10 vials

Maurice CE-SDS Running Buer – Bottom 15 mL

Maurice CE-SDS 1X Sample Buer 25 mL

Maurice CE-SDS Wash Solution 2 x 20 mL

Maurice CE-SDS Conditioning Solution 1 20 mL

Maurice CE-SDS Conditioning Solution 2 20 mL

Storage conditions

• Store the 25X Internal Standard (P/N 046-144) at 2-8 °C.

Everything else should be stored at room temperature.

• Separation and Running buer bottles should be tightly

closed to prevent evaporation.

• Running Buer

– Top vials must be stored tightly closed

in the original container with two humidifying pouches.

Ordering info

This kit can be reordered by:

• Phone: [phone], option 1

• Fax: [phone]

• Email: [e-mail]

Other things you’ll need

• Maurice CE-SDS IgG Standard, PN046-039

• Maurice CE-SDS Molecular Weight Markers, PN046-432

(optional)

• Maurice sample vials with integrated inserts, 0.2mL,

PN046-083 (optional)

• β-mercaptoethanol (>98% = 14.2 M) for reducing

conditions

• Iodoacetamide (250 mM) for alkylation at non-reducing

conditions

• Deionized (DI) water

Maurice CE-SDS 25X Internal Standard 2 vials

• Pipettes and tips

• Microcentrifuge and tubes

• Ice and ice bucket

• Vortexer

• Water bath or thermocycler

• Centrifuge with plate adapter or vial adapter (12mm,

2mL vials)

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Maurice CE-SDS Application Guide

Guidelines for great results

• Make sure you read the entire Application Guide before

getting started.

• Use fresh Conditioning Solutions, Separation Matrix, and

Wash Solution for each batch.

• When the Top Running Buer vial is still in the cartridge

insert, the cartridge MUST be kept in an upright position
at all times.

• The 25X Internal Standard, IgG Standard, and CE-SDS

Molecular Weight Markers are lyophilized. Always
reseal the foil bag containing the unopened tubes with
desiccant to prevent moisture absorption.

• Aliquot the reconstituted 25X Internal Standard solution

into appropriately sized vials and store at -80 °C for
long-term storage. For short-term storage (<1 week), the
solution can be stored at 2–8 °C.

• If you observe any precipitation in the 25X Internal

Standard solution, then leave the solution at room
temperature and stir gently until the precipitates have
dissolved completely.

• Whenever you handle the cartridge or remove it from

its packaging, make sure the cartridge inlet doesn’t
come in contact with any surfaces. A damaged inlet may
compromise the cartridge and cause a failed injection.

• Always perform the cartridge cleanup before storing,

and always store the cartridge in its original packaging at
room temperature.

• If you see any Separation Matrix sticking to the cartridge

inlet, soak the inlet for 5 min in DI water. Then wipe
the inlet using a lint-free laboratory wipe that’s been
moistened with DI water.

• Each cartridge is guaranteed for up to 100 injections and

maxium of 25 batches. The injection limit of the cartridge
is 200. Its RFID will keep track of how many injections
have been performed and how many are left for you.

• Don’t reuse reagents, vials or the clear screw caps.

Always keep the orange pressure caps paired with their
respective reagents.

• Orange pressure caps should be used for the CE-SDS

application only.

• If you want to reuse the orange pressure caps, wash

them thoroughly with DI water, soak them overnight in
DI water, rinse caps thoroughly with DI water again and
air dry them before reusing.

Application overview

A successfully dened and optimized CE-SDS method
gives you:

• A highly reproducible peak prole

• Reproducible relative migration times for each peak

• NGHC/HC baseline peak resolution (≥1.5) for the reduced

IgG sample

3

Maurice CE-SDS Application Guide

Step 1:

Step 2:

Step3:

Run ProteinSimple
reduced IgG Standard

Pass

Run sample

Yes

Current ~25 µA?

Yes

All peaks detected?

Yes

Fail

No

No

Call ProteinSimple

Tech Support

• Make sure separation reagents
are in correct vial positions

• Always use fresh reagents

• Optimize salt and protein
concentration in the sample

• Verify denaturing conditions

• Optimize injection and
separation parameters (time
and voltage)

Resolution sucient?

Yes

Method development

is complete

FIGURE 1. Method development strategy.

No

• Check conditioning reagents

• Perform a cartridge cleanup

• Optimize injection parameters
(time and voltage)

4