ProteinSimple Maurice S, Maurice C, Maurice User Manual

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User Guide for Maurice, Maurice C. and Maurice S.

ProteinSimple 3001 Orchard Parkway San Jose, CA 95134 Toll-free: (888) 607-9692 Tel: (408) 510-5500 Fax: (408) 510-5599 email: support@proteinsimple.com web: www.proteinsimple.com

User Guide for Maurice, Maurice C. and Maurice S.

P/N 046-295

Revision 10, April 2018

For research use only. Not for use in diagnostic procedures

Patents and Trademarks

ProteinSimple's Maurice and iCE technology is covered by issued and pending patents in the U.S. and other countries. For more information please see http://www.proteinsimple.com/Intellectual_Property.html.

ProteinSimple and the ProteinSimple logo are trademarks or registered trademarks of ProteinSimple. Other marks appearing in these materials are marks of their respective owners.

page 1

Chapter 1:

Let’s Get Started. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Welcome . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Maurice Systems. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Chapter 2:

Getting Your Lab Ready . . . . . . . . . . . . . . . . . . . . 3

Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Space Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Physical Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Environmental Requirements. . . . . . . . . . . . . . . . . . . . . . . . . 5

Software and Computer Requirements . . . . . . . . . . . . . . . 6

General Guidelines and Information. . . . . . . . . . . . . . . . . . 6

Intended Use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Lifting and Moving the System: Lift Maurice

Correctly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Chapter 3:

Maurice. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Maurice Systems. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

External Components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

System Door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Status Light . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10

Internal Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Cartridge Slot . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10

Sample and Reagent Platform . . . . . . . . . . . . . . . . . . 11

Rear Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

System Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15

Computer Workstation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Compass for iCE Overview . . . . . . . . . . . . . . . . . . . . . . . . . . .19

Changing the Screen View . . . . . . . . . . . . . . . . . . . . . .20

Batch Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Run Summary Screen . . . . . . . . . . . . . . . . . . . . . . . . . . .21

Analysis Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23

Screen Panes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25

Title Bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Main Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Instrument Status Bar . . . . . . . . . . . . . . . . . . . . . . . . . . .25

Screen Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26

View Bar. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Compass for iCE Status Bar. . . . . . . . . . . . . . . . . . . . . . 26

Software Menus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26

File Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27

Edit Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

View Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28

Instrument Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28

Window Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

Help Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

Changing the Compass for iCE Main Window

Layout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

Resizing the Main Compass for iCE Window . . . . 29

Resizing the Screen Tab . . . . . . . . . . . . . . . . . . . . . . . . .30

Resizing Screen Panes. . . . . . . . . . . . . . . . . . . . . . . . . . .30

Changing the Location of Screen Panes. . . . . . . . .31

Restoring the Main Window to the Default

Layout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33

Software Help. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33

Chapter 4:

Compass for iCE Overview . . . . . . . . . . . . . . . . 18

Launching Compass for iCE . . . . . . . . . . . . . . . . . . . . . . . . . 19

User Guide for Maurice, Maurice C. and Maurice S.

Checking for and Installing New Versions of Compass

for iCE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

Viewing Release Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33

page 2

Viewing the Software Log. . . . . . . . . . . . . . . . . . . . . . . . . . . .34

Compass for iCE Version Information . . . . . . . . . . . . . . . .34

Directory and File Information. . . . . . . . . . . . . . . . . . . . . . . 35

File Types. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

Chapter 5:

cIEF Batches. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

Batch Screen Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39

Batch Screen Panes . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39

Software Menus Active in the Batch Screen . . . . .40

Opening a Batch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42

Creating a New Batch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

Step 1 - Open a Template Batch. . . . . . . . . . . . . . . . .43

Step 2 - Assign Your Samples. . . . . . . . . . . . . . . . . . . .44

Step 3 - Assign Your Method Parameters. . . . . . . .47

Step 4 - Set Up Your Injections. . . . . . . . . . . . . . . . . . .51

Step 5 - Setup On-Board Mixing (Optional) . . . . .55

Step 6 - Add Programmed Pauses and Stops

(Optional). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

Step 7 - Add Batch Notes (Optional). . . . . . . . . . . . .60

Step 8 - Modify Default Analysis Parameters

(Optional). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Exporting a Method. . . . . . . . . . . . . . . . . . . . . . . . . . . . .72

Batch Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73

Chapter 6:

CE-SDS Batches . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

Batch Screen Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

Batch Screen Panes . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77

Software Menus Active in the Batch Screen . . . . .78

Opening a Batch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

Creating a New Batch. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81

Step 1 - Open a Template Batch. . . . . . . . . . . . . . . . .81

Step 2 - Assign Your Samples. . . . . . . . . . . . . . . . . . . .82

Step 3 - Assign Your Method Parameters . . . . . . . .85

Step 4 - Set Up Your Injections. . . . . . . . . . . . . . . . . . .87

Step 5 - Add Programmed Pauses and Stops

(Optional). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91

Step 6 - Add Batch Notes (Optional). . . . . . . . . . . . .92

Step 7 - Modify Default Analysis Parameters

(Optional). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92

Step 8 - Save Your Batch . . . . . . . . . . . . . . . . . . . . . . . .93

Viewing Replicate Injections . . . . . . . . . . . . . . . . . . . . . . . . .94

Batch History . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95

Step 9 - Save Your Batch . . . . . . . . . . . . . . . . . . . . . . . .61

Viewing Replicate Injections . . . . . . . . . . . . . . . . . . . . . . . . . 62

On-board Mixing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63

Batch History . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .66

Making Changes to a Batch . . . . . . . . . . . . . . . . . . . . . . . . .67

Viewing and Editing Batches in Completed Runs . . . . 68

Copying and Pasting Injection Names or Sample IDs

from other Documents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69

Importing and Exporting Methods. . . . . . . . . . . . . . . . . . . 71

Importing a Method . . . . . . . . . . . . . . . . . . . . . . . . . . . .72

User Guide for Maurice, Maurice C. and Maurice S.

Making Changes to a Batch . . . . . . . . . . . . . . . . . . . . . . . . .96

Viewing and Editing Batches in Completed Runs . . . .97

Copying and Pasting Injection Names or Sample IDs

from other Documents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .98

Importing and Exporting Methods. . . . . . . . . . . . . . . . . 100

Importing a Method . . . . . . . . . . . . . . . . . . . . . . . . . . 101

Exporting a Method. . . . . . . . . . . . . . . . . . . . . . . . . . . 101

Batch Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102

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Chapter 7: Running cIEF Applications on

Maurice and Maurice C.. . . . . . . . . . . . . . . . . . .106

Before You Throw the Switch . . . . . . . . . . . . . . . . . . . . . . .107

Power Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .107

Running cIEF Applications . . . . . . . . . . . . . . . . . . . . . . . . . .107

What You’ll Need . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .107

Step 1: Prep Your Markers, Samples and

Reagents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .108

Step 2: Prep the Cartridge . . . . . . . . . . . . . . . . . . . . . .112

Step 3: Install the Cartridge. . . . . . . . . . . . . . . . . . . . .114

Step 4: Load Samples and Reagents . . . . . . . . . . .115

Step 5: Create a Batch. . . . . . . . . . . . . . . . . . . . . . . . . .117

Step 6: Start the Batch. . . . . . . . . . . . . . . . . . . . . . . . . .131

Editing a Running Batch. . . . . . . . . . . . . . . . . . . . . . . . . . . .133

Post-batch Procedures. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .135

Checking Your Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .138

Step 3: Install the Cartridge. . . . . . . . . . . . . . . . . . . . 155

Step 4: Load Samples and Reagents. . . . . . . . . . . 157

Step 5: Create a Batch. . . . . . . . . . . . . . . . . . . . . . . . . 159

Step 6: Start the Batch. . . . . . . . . . . . . . . . . . . . . . . . . 169

Editing a Running Batch . . . . . . . . . . . . . . . . . . . . . . . . . . . 171

Post-batch Procedures. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173

Checking Your Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 177

Step 1: Check Your Internal Standard . . . . . . . . . 177

Step 2: Set Your Molecular Weight (MW)

Markers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 181

Step 3: Checking Sample Peaks . . . . . . . . . . . . . . . 185

Step 4: Assigning Peak Names. . . . . . . . . . . . . . . . . 188

Chapter 9:

Run Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 189

Run Summary Screen Overview. . . . . . . . . . . . . . . . . . . . 190

Run Summary Screen Panes . . . . . . . . . . . . . . . . . . 190

Step 1: Select Your Detection Mode . . . . . . . . . . . .138

Step 2: Check Your pI Markers . . . . . . . . . . . . . . . . . .138

Step 3: Checking Sample Peaks . . . . . . . . . . . . . . . .141

Step 4: Assigning Peak Names . . . . . . . . . . . . . . . . .144

Chapter 8: Running CE-SDS Applications on

Maurice and Maurice S. . . . . . . . . . . . . . . . . . . .145

Before You Throw the Switch . . . . . . . . . . . . . . . . . . . . . . .146

Power Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .146

Running CE-SDS Applications . . . . . . . . . . . . . . . . . . . . . .146

What You’ll Need . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .146

Step 1: Prep Your Internal Standard, Samples and

Reagents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .147

Internal Standard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .147

Step 2: Prep the Cartridge . . . . . . . . . . . . . . . . . . . . . .153

User Guide for Maurice, Maurice C. and Maurice S.

Software Menus Active in the Run Summary

Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 192

Opening Run Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 194

Opening One Run File. . . . . . . . . . . . . . . . . . . . . . . . . 194

Opening Multiple Run Files. . . . . . . . . . . . . . . . . . . . 194

Batch Injection Information . . . . . . . . . . . . . . . . . . . . . . . 195

Injection Flags . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 197

Run Status Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . 197

Viewing the Focus Series (cIEF Only) . . . . . . . . . . . . . . . 199

Viewing the Separation (CE-SDS Only) . . . . . . . . . . . . . 200

Current and Voltage Plots. . . . . . . . . . . . . . . . . . . . . . . . . . 201

Run History . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203

Viewing Multiple Events. . . . . . . . . . . . . . . . . . . . . . . 203

Copying History Info . . . . . . . . . . . . . . . . . . . . . . . . . . 205

Viewing Run Errors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 205

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Injection Reports. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .206

Example Analysis and Injection Report: CE-SDS208

Example Analysis and Injection Report: cIEF. . . .211

Switching Between Open Run Files . . . . . . . . . . . . . . . . .213

Closing Run Files. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .214

Chapter 10: Controlling Maurice, Maurice C. and

Maurice S. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .215

Instrument Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .216

Starting a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .216

Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .216

Stopping a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .219

Emergency Stop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .220

Status Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .220

Shutdown . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .221

Instrument Software (Embedded) Updates . . . . . . . . .221

Self Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .222

Viewing and Changing System Properties . . . . . . . . . .223

Checking Cartridge Status . . . . . . . . . . . . . . . . . . . . . . . . . .224

Instrument Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .225

Example Instrument Report . . . . . . . . . . . . . . . . . . . .227

Viewing Log Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .231

Runs Log . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .231

Instrument Log . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .232

Self Test Logs. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .235

Command Log. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .237

Software Menus Active in the Analysis Screen . 241

Opening Run Files. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 243

Opening One Run File. . . . . . . . . . . . . . . . . . . . . . . . . 243

Opening Multiple Run Files. . . . . . . . . . . . . . . . . . . . 243

How Run Data is Displayed . . . . . . . . . . . . . . . . . . . . . . . . 245

Experiment Pane: Batch Injection Information 245

Graph Pane: Electropherogram Data . . . . . . . . . 246

Peaks Pane: Calculated Results. . . . . . . . . . . . . . . . 246

Injections Pane: User-Specified Peak Names. . . 249

Viewing Run Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 251

Switching Between Samples and Standards Data

Views. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 251

Selecting and Displaying Injection Data . . . . . . 254

Switching Between Single and Multiple Views of

Injections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257

Hiding Injection Data . . . . . . . . . . . . . . . . . . . . . . . . . 259

Data Notifications and Warnings. . . . . . . . . . . . . . . . . . 260

Checking Your Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 261

Group Statistics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 262

Using Groups. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 262

Viewing Sample Injection Groups . . . . . . . . . . . . . 263

Viewing Statistics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 263

Hiding or Removing Injections in Group

Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 265

Copying Results Tables and Graphs. . . . . . . . . . . . . . . . 266

Copying Results Tables . . . . . . . . . . . . . . . . . . . . . . . . 266

Copying the Graph. . . . . . . . . . . . . . . . . . . . . . . . . . . . 266

Chapter 11:

CE-SDS Data Analysis . . . . . . . . . . . . . . . . . . . . .239

Analysis Screen Overview . . . . . . . . . . . . . . . . . . . . . . . . . . .240

Analysis Screen Panes. . . . . . . . . . . . . . . . . . . . . . . . . .240

User Guide for Maurice, Maurice C. and Maurice S.

Saving the Graph as an Image File . . . . . . . . . . . . 266

Exporting Run Files. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 267

Exporting Results Tables. . . . . . . . . . . . . . . . . . . . . . . 267

page 5

Exporting Raw Sample Electropherogram

Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .267

Changing Sample Protein Identification. . . . . . . . . . . .268

Adding or Removing Sample Data. . . . . . . . . . . . .268

Hiding Sample Data . . . . . . . . . . . . . . . . . . . . . . . . . . .270

Changing Peak Names for Sample Data . . . . . . .271

Changing the Electropherogram View. . . . . . . . . . . . . .272

Autoscaling the Electropherogram . . . . . . . . . . . .273

Customizing the Data Display . . . . . . . . . . . . . . . . .273

Stacking Multiple Electropherograms. . . . . . . . . .279

Overlaying Multiple Electropherograms . . . . . . .280

Zooming. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .281

Selecting Data Viewing Options . . . . . . . . . . . . . . .282

Adding and Removing Baseline Points. . . . . . . . .286

Selecting the Graph X-axis Range . . . . . . . . . . . . . .287

Closing Run Files. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .289

Analysis Settings Overview. . . . . . . . . . . . . . . . . . . . . . . . . .290

Advanced Analysis Settings. . . . . . . . . . . . . . . . . . . . . . . . .292

Internal Standard Settings . . . . . . . . . . . . . . . . . . . . .292

Changing the Default Standards Group . . . . . . 304

Modifying a Standards Group. . . . . . . . . . . . . . . . . 304

Deleting a Standards Group . . . . . . . . . . . . . . . . . . 304

Applying Standards Groups to Specific Run

Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 305

Peak Fit Analysis Settings . . . . . . . . . . . . . . . . . . . . . . . . . . 307

Range Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 308

Baseline Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 309

Peak Find Settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 309

Peak Fit Analysis Settings Groups. . . . . . . . . . . . . . 310

Creating a New Peak Fit Group. . . . . . . . . . . . . . . . 311

Changing the Default Peak Fit Group . . . . . . . . . 313

Modifying a Peak Fit Group . . . . . . . . . . . . . . . . . . . 313

Deleting a Peak Fit Group . . . . . . . . . . . . . . . . . . . . . 314

Applying Peak Fit Groups to Specific Run Data 314

Manual Peak Integration . . . . . . . . . . . . . . . . . . . . . . . . . . 317

Peak Names Settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 321

Peak Names Analysis Settings Groups. . . . . . . . . 321

Creating a Peak Names Group . . . . . . . . . . . . . . . . 322

Advanced Analysis Settings Groups . . . . . . . . . . . .293

Creating a New Analysis Group . . . . . . . . . . . . . . . .293

Changing the Default Analysis Group. . . . . . . . . .294

Modifying an Analysis Group . . . . . . . . . . . . . . . . . .295

Deleting an Analysis Group . . . . . . . . . . . . . . . . . . . .295

Applying Analysis Groups to Specific Run Data 296

Markers Analysis Settings . . . . . . . . . . . . . . . . . . . . . . . . . . .298

Markers Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .299

Changing the Injection Used for the CE-SDS MW

Markers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .299

Standards Analysis Settings Groups. . . . . . . . . . . .300

Creating a New Standards Group . . . . . . . . . . . . . .301

User Guide for Maurice, Maurice C. and Maurice S.

Modifying a Peak Names Group . . . . . . . . . . . . . . 325

Deleting a Peak Names Group . . . . . . . . . . . . . . . . 325

Applying Peak Names Groups to Run Data . . . 326

Injection Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 328

Example Analysis and Injection Report. . . . . . . . 330

Importing and Exporting Analysis Settings. . . . . . . . . 333

Importing Analysis Settings . . . . . . . . . . . . . . . . . . . 333

Exporting Analysis Settings. . . . . . . . . . . . . . . . . . . . 333

Chapter 12:

cIEF Data Analysis. . . . . . . . . . . . . . . . . . . . . . . . . 335

Analysis Screen Overview . . . . . . . . . . . . . . . . . . . . . . . . . . 337

Analysis Screen Panes . . . . . . . . . . . . . . . . . . . . . . . . . 337

page 6

Software Menus Active in the Analysis Screen . .338

Opening Run Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .340

Opening One Run File. . . . . . . . . . . . . . . . . . . . . . . . . .340

Opening Multiple Run Files. . . . . . . . . . . . . . . . . . . . .340

How Run Data is Displayed . . . . . . . . . . . . . . . . . . . . . . . . .342

Experiment Pane: Batch Injection Information .342

Graph Pane: Electropherogram Data . . . . . . . . . .342

Peaks Pane: Calculated Results. . . . . . . . . . . . . . . . .343

Injections Pane: User-Specified Peak Names . . .345

Viewing Run Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .346

Switching Between Samples and Markers Data

Views . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .346

Selecting and Displaying Injection Data . . . . . . .349

Switching Between Single and Multiple Views of

Injections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .352

Hiding Injection Data . . . . . . . . . . . . . . . . . . . . . . . . . .354

Data Notifications and Warnings. . . . . . . . . . . . . . . . . . .355

Checking Your Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .356

Group Statistics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .356

Using Groups . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .357

Viewing Sample Injection Groups . . . . . . . . . . . . . .357

Viewing Statistics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .358

Hiding or Removing Injections in Group

Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .360

Exporting Raw Sample Electropherogram

Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 362

Changing Sample Protein Identification . . . . . . . . . . . 363

Adding or Removing Sample Data. . . . . . . . . . . . 363

Hiding Sample Data . . . . . . . . . . . . . . . . . . . . . . . . . . 365

Changing Peak Names for Sample Data . . . . . . 366

Changing the Electropherogram View . . . . . . . . . . . . . 367

Autoscaling the Electropherogram. . . . . . . . . . . . 368

Customizing the Data Display . . . . . . . . . . . . . . . . 368

Stacking Multiple Electropherograms . . . . . . . . . 374

Overlaying Multiple Electropherograms. . . . . . . 375

Overlaying Fluorescence and Absorbance

Electropherograms. . . . . . . . . . . . . . . . . . . . . . . . . . . . 375

Zooming . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 377

Selecting Data Viewing Options. . . . . . . . . . . . . . . 378

Adding and Removing Baseline Points . . . . . . . . 382

Selecting the Graph X-axis Range . . . . . . . . . . . . . 383

Closing Run Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 385

Analysis Settings Overview. . . . . . . . . . . . . . . . . . . . . . . . . 386

Advanced Analysis Settings . . . . . . . . . . . . . . . . . . . . . . . . 388

pI Markers Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . 388

Advanced Analysis Settings Groups . . . . . . . . . . . 389

Creating a New Analysis Group . . . . . . . . . . . . . . . 389

Changing the Default Analysis Group. . . . . . . . . 390

Copying Results Tables and Graphs. . . . . . . . . . . . . . . . .361

Copying Results Tables. . . . . . . . . . . . . . . . . . . . . . . . .361

Copying the Graph. . . . . . . . . . . . . . . . . . . . . . . . . . . . .361

Saving the Graph as an Image File. . . . . . . . . . . . .361

Exporting Run Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .362

Exporting Results Tables . . . . . . . . . . . . . . . . . . . . . . .362

User Guide for Maurice, Maurice C. and Maurice S.

Modifying an Analysis Group. . . . . . . . . . . . . . . . . . 391

Deleting an Analysis Group . . . . . . . . . . . . . . . . . . . 391

Applying Analysis Groups to Specific Run Data 392

Detection Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 395

Changing the Detection Method. . . . . . . . . . . . . . 395

Changing the Detection Exposure . . . . . . . . . . . . 396

Peak Fit Analysis Settings . . . . . . . . . . . . . . . . . . . . . . . . . . 397

page 7

Range Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .398

Baseline Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .398

Peak Find Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . .398

Peak Fit Analysis Settings Groups . . . . . . . . . . . . . .400

Creating a New Peak Fit Group. . . . . . . . . . . . . . . . .400

Changing the Default Peak Fit Group . . . . . . . . . .402

Modifying a Peak Fit Group . . . . . . . . . . . . . . . . . . . .402

Deleting a Peak Fit Group . . . . . . . . . . . . . . . . . . . . . .403

Applying Peak Fit Groups to Specific Run Data.403

Manual Peak Integration . . . . . . . . . . . . . . . . . . . . . . . . . . .406

Peak Names Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .411

Peak Names Analysis Settings Groups. . . . . . . . . .411

Creating a Peak Names Group . . . . . . . . . . . . . . . . .412

Modifying a Peak Names Group . . . . . . . . . . . . . . .415

Deleting a Peak Names Group . . . . . . . . . . . . . . . . .415

Applying Peak Names Groups to Run Data . . . .416

pI Markers Analysis Settings. . . . . . . . . . . . . . . . . . . . . . . . .419

pI Markers Analysis Settings Groups. . . . . . . . . . . .419

Creating a New Markers Group . . . . . . . . . . . . . . . .420

Changing the Default Markers Group. . . . . . . . . .422

Modifying a Markers Group . . . . . . . . . . . . . . . . . . . .423

Deleting a Markers Group. . . . . . . . . . . . . . . . . . . . . .423

Applying Markers Groups to Specific Run Data.424

Injection Reports. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .426

Example Analysis and Injection Report. . . . . . . . .428

Importing and Exporting Analysis Settings . . . . . . . . .431

Importing Analysis Settings . . . . . . . . . . . . . . . . . . . .431

Exporting Analysis Settings . . . . . . . . . . . . . . . . . . . .431

Chapter 13:

Setting Your Preferences. . . . . . . . . . . . . . . . . 433

Customize Your Preferences . . . . . . . . . . . . . . . . . . . . . . . 434

Enabling Access Control . . . . . . . . . . . . . . . . . . . . . . . . . . . 435

Setting Data Export Options . . . . . . . . . . . . . . . . . . . . . . . 435

Setting up Automatic Injection Reports . . . . . . . . . . . . 436

Selecting Custom Plot Colors for Graph Overlay . . . 437

Grouping Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 439

Setting Up Maurice Systems to Send Tweets . . . . . . . 440

Chapter 14: Compass Access Control and

21 CFR Part 11 Compliance . . . . . . . . . . . . . . 445

Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 446

Authorization Server . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 446

Server Administration . . . . . . . . . . . . . . . . . . . . . . . . . 447

Adding Non-admin Users . . . . . . . . . . . . . . . . . . . . . 448

Adding Admin Users . . . . . . . . . . . . . . . . . . . . . . . . . . 452

Resetting User Passwords . . . . . . . . . . . . . . . . . . . . . 453

Audit Trail . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 454

Password Policy Settings . . . . . . . . . . . . . . . . . . . . . . 456

LDAP Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 456

Encryption Details. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 457

Enabling Access Control . . . . . . . . . . . . . . . . . . . . . . . . . . . 458

Logging In to Compass for iCE . . . . . . . . . . . . . . . . . . . . . 459

Locking and Unlocking the Application . . . . . . . 460

Resolving Log In Issues . . . . . . . . . . . . . . . . . . . . . . . . 460

Saving Changes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 461

Signing Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 462

Instrument Command Log . . . . . . . . . . . . . . . . . . . . . . . . 462

User Guide for Maurice, Maurice C. and Maurice S.

Run File History . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 465

page 8

Troubleshooting Problems and Suggested

Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .465

Chapter 15:

Maintenance and Troubleshooting . . . . .467

Cartridge Handling and Care . . . . . . . . . . . . . . . . . . . . . . .468

cIEF Cartridge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .469

CE-SDS Cartridge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .470

Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .471

Daily . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .471

Yearly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .471

Changing the Fuse. . . . . . . . . . . . . . . . . . . . . . . . . . . . .471

Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .473

Software Updates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .473

Instrument Software (Embedded) Updates . . . . . . . . .473

Frequently Asked Questions: cIEF Applications. . . . . .474

Frequently Asked Questions: CE-SDS Applications . .478

Maurice cIEF Consumables, Kits and Reagents 491

Maurice Systems Consumables and Reagents 493

Customer Service and Technical Support . . . . . . . . . . 493

Legal Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 495

Maurice Disclaimer of Warranty . . . . . . . . . . . . . . 495

Compass Software and Authorization Server

License Agreement . . . . . . . . . . . . . . . . . . . . . . . . . . . . 495

Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .478

cIEF Application Troubleshooting. . . . . . . . . . . . . .479

CE-SDS Application Troubleshooting . . . . . . . . . .481

Chapter 16:

General Information . . . . . . . . . . . . . . . . . . . . . .483

Compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .484

Safety Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .485

Chemical Hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . .485

Chemical Waste Hazards . . . . . . . . . . . . . . . . . . . . . .486

Waste Production and Disposal. . . . . . . . . . . . . . . .487

Safety Data Sheets . . . . . . . . . . . . . . . . . . . . . . . . . . . . .487

Instrument Safety Labels. . . . . . . . . . . . . . . . . . . . . . .488

Consumables and Reagents . . . . . . . . . . . . . . . . . . . . . . . .489

Maurice CE-SDS Consumables, Kits and

Reagents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .489

User Guide for Maurice, Maurice C. and Maurice S.

Chapter 1:

Let’s Get Started

Chapter Overview

•Welcome

page 1

•Maurice Systems

User Guide for Maurice, Maurice C. and Maurice S.

Welc ome page 2

Welcome

Congratulations on bringing Maurice into your lab! We welcome you as a new user and are excited to be a part of your work. This user guide will provide you with details on system hardware, operating the system, how to use Compass for iCE software, maintenance procedures and other useful information.

To help you get the most from you new lab addition, we've added some attention phrases to guide you through the user guide:

NOTE Points out useful information.

IMPORTANT Indicates information necessary for proper operation of Maurice systems.

CAUTION Cautions you about potentially hazardous situations that could result in injury to you or

damage to the system.

!WARNING! Warns you that serious physical injury can result if the listed precautions aren’t followed.

Maurice Systems

Maurice, Maurice C. and Maurice S. systems give you identity, purity and heterogeneity data on your biologics, and get you to results faster with short development times and simple workflows!

• They're fluent in cIEF and CE-SDS. They take cIEF up a notch, and CE-SDS is a breeze. You'll get pI and charge heterogeneity data in less than 10 minutes flat — with the added bonus of same-time absorbance and native fluorescence for sensitivity down to 0.7 μg/mL. Their size applications have the high res and wide molecular weight range you need and they're done in 35 minutes.

• They make it easy. Just pop in a ready-to-go cartridge, drop in your sample vials or a 96-well plate, and hit start — they'll do the rest!

• They're time-savers. Develop methods fast so you get to results even faster. Your cIEF and CE-SDS methods are done in a day. The icing? You can develop platform methods and use them for multiple molecules. No maintenance and clean-up needed between the two applications.

• They're dependable. Get reproducible results with tight CVs day in and day out. Your data is reliable no matter what — across samples, users, instruments or labs.

User Guide for Maurice, Maurice C. and Maurice S.

Chapter 2:

Getting Your Lab Ready

Chapter Overview

•Introduction

page 3

• Space Requirements

• Physical Specifications

• Electrical Requirements

• Environmental Requirements

• Software and Computer Requirements

• General Guidelines and Information

User Guide for Maurice, Maurice C. and Maurice S.

Introduction page 4

Introduction

This chapter will help you prepare the lab for Maurice. Please have the space, electrical and environmental requirements ready prior to scheduling your installation.

NOTE: Please wait for an authorized ProteinSimple Field Service Engineer to unpack and install Maurice for you. Don’t try doing this yourself. Handling Maurice incorrectly could cause injury to yourself or damage to the system.

Space Requirements

You need a lab bench or table that can support 100 lb (46 kg) and has enough space for both Maurice and his computer. There should be sufficient clearance for both heat ventilation and to provide access if Maurice needs service.

IMPORTANT

Maurice needs a stable surface and must remain level to work properly. The lab bench or table can’t shift or wobble under heavy weight. Don’t use anti-vibration tables either, since Maurice may not stay level while he’s working.

Dimension Meters Feet

Width 1.5 5.0

Depth 0.8 2.5

Height 0.5 1.5

Recommended space requirements for Maurice.

User Guide for Maurice, Maurice C. and Maurice S.

Physical Specifications page 5

Physical Specifications

Description Specification

Maurice’s Dimensions (Door Closed) 0.44 m x 0.42 m x 0.61m (H x W x D)

1.46’ x 1.38’ x 2.0’ (H x W x D)

Maurice’s Dimensions (Door Open) 0.44 m x 0.57 m x 0.61m (H x W x D)

1.46” x 2.43’ x 2.0’ (H x W x D)

Maurice’s Weight 46 kg (100 lb)

Computer Workstation Dimensions 0.41 m x 0.66 m x 0.76 m (H x W x D)

1.35' x 2.17' x 2.49' (H x W x D)

For indoor use only. Use up to altitudes of 1524 meters (5000 feet).

Table 2-1: Physical Specifications

Electrical Requirements

Maurice requires a dedicated, grounded circuit capable of delivering the appropriate current and voltage for your country. The power requirements for all three Maurice systems are 100 V- 240 V (AC), 50/60 Hz, 500 W.

In addition to these requirements, Maurice needs the grounded circuits terminate at the receptacles, and receptacles must be located within 10 ft (3 m) of the instrument.

Environmental Requirements

Maurice likes a consistent temperature in the lab (not too hot – not too cold). He works best when conditions stay within these ranges:

Requirement Specification

Operating temperature range 18 - 25 °C (64 - 77 °F)

Operating humidity range 20-80% relative, non-condensing

Table 2-2: Environmental requirements.

User Guide for Maurice, Maurice C. and Maurice S.

Software and Computer Requirements page 6

Software and Computer Requirements

Maurice brings his own computer to the lab with Compass for iCE software pre-installed. Compass for iCE is used to run cIEF and CE-SDS applications on Maurice and analyze resulting data. Just in case you need it, a CD containing Compass for iCE software also comes in the box. If you don't want to analyze your data at Maurice's workstation in the lab, Compass for iCE software can also be installed on a separate workstation, such as your desktop computer. Your computer must meet the recommended requirements listed below to run Compass for iCE software and process data.

Component Minimum Requirements

Operating System Windows 7

Processor Core i5

Memory 4 GB

Free Disk Space 100 GB

Ethernet Ports 2 - One is required to connect to Maurice, the other is

used for network access

USB Ports 2 - To connect the keyboard and mouse

Table 2-3: Computer requirements.

General Guidelines and Information

Intended Use

NOTE: Maurice is for research use only. Not for use in diagnostic procedures.

Lifting and Moving the System: Lift Maurice Correctly

IMPORTANT

Take all the standard precautions when lifting or moving Maurice. Since Maurice systems weigh 46 kg (100 lb), you should not lift him by yourself. Two people should lift him onto the lab bench.

User Guide for Maurice, Maurice C. and Maurice S.

Chapter 3:

Maurice

Chapter Overview

•Maurice Systems

page 7

• External Components

• Internal Components

•Rear Panel

•Computer Workstation

User Guide for Maurice, Maurice C. and Maurice S.

Maurice Systems page 8

Maurice with Computer Workstation

cIEF and CE-SDS Cartridges

Maurice C. Maurice Maurice S.

Maurice Systems

Maurice, Maurice C. and Maurice S. systems include the instrument, computer workstation, Compass for iCE software and cIEF or CE-SDS Cartridges.

All systems have the same hardware components, computer and software, the only difference between them are the applications you can run:

• Maurice: cIEF and CE-SDS applications

• Maurice C.: cIEF applications only

• Maurice S.: CE-SDS applications only

You can run samples in 96-well plates or in up to 48 sample vials with integrated 0.2 mL inserts on all three systems.

User Guide for Maurice, Maurice C. and Maurice S.

External Components page 9

Tou ch Plat e to

System Door

Status Light

Open Door

External Components

!WARNING!

You can’t replace or service any parts on Maurice systems except for the power entry fuse.

System Door

Maurice's door gives you access to the inside of the instrument to load cartridges, reagents and samples. To open the door, first make sure the status light is a steady blue. Then just touch the metal touch plate on the top of the door to open it. Close it by pushing the door until you hear the latch engage.

NOTE: Maurice’s door must be closed before starting a batch.

User Guide for Maurice, Maurice C. and Maurice S.

Internal Components page 10

Status Light

The LED on Maurice’s front panel tells you what he’s doing. Here’s what his different status lights mean:

• Start-up (magenta): You've just turned on the power and Maurice is warming up.

• Ready (steady blue): Maurice is powered on and ready to go.

• Opening Door (long blue flash followed by blue pulses): Maurice’s door is opening.

• Running (pulsing blue): Maurice is running a batch.

• Paused (blue pulse with magenta flashes): The batch is paused. You can edit the batch and open Maurice's door to add new samples or reagents.

• Trying to Open Door While Running (red flash): Maurice’s door can’t be opened when he’s running.

• Error (steady red): Maurice has detected an error. To get more information on the error, check the Status pane in the Run Summary Screen in Compass for iCE.

Internal Components

Cartridge Slot

The cartridge slot holds Maurice’s ready-to-go application cartridges. The cartridge it holds depends on the system:

• Maurice: cIEF and CE-SDS Cartridges

• Maurice C.: cIEF Cartridges only

• Maurice S.: CE-SDS Cartridges only

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Internal Components page 11

Cartridge

Slot

The lights on either side of the cartridge slot will be orange after Maurice disengages the cartridge when the door is opened at the end of a batch, and whenever the slot is empty.

The lights change to blue once a cartridge is installed correctly.

NOTE: You can find cartridge prep, installation and post-run procedures in Chapter 7, “Running cIEF Applications on Maurice and Maurice C.“ and Chapter 8, “Running CE-SDS Applications on Maurice and Maurice S.“

Sample and Reagent Platform

Maurice’s sample and reagent platform has two rows for batch reagents. These reagents are kept at room temperature.

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Internal Components page 12

• Row P (top): These reagents are loaded under pressure during the batch. Only use glass reagent vials with pressure caps in this row. Use blue pressure caps with cIEF reagents and orange pressure caps with CE-SDS reagents.

• Row N (bottom): Only use reagent vials with clear screw caps in this row.

The sample block holds either a 96-well plate or 48-vial metal insert and is temperature-controlled. You can set it to 4 °C, 10 °C, 15 °C or turn the temperature control off in Compass for iCE software.

Sample cooling turns on when the run starts, and takes a few minutes to reach the temperature setting. After a run, the sample block stays at the set temperature until you open Maurice’s door, then it shuts off until you start the next run. This prevents excess condensation.

NOTE: Because Maurice holds the sample block temperature after a run until you open the door, samples are still viable for your next run and after overnight runs.

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Internal Components page 13

96-well Plate

Insert

48-vial

Insert

OBM Reagent Positions in

96-well Plate Insert

OBM Reagent Positions in

48-vial Insert

Maurice-OBM and Maurice C.-OBM instruments will also have four additional reagent locations in the 96well plate and 48-vial metal inserts. These reagents are also temperature controlled at the same setting used for the samples.

• Column M (left): These reagents are used for on-board mixing. Only use 6 mL reagent vials in this row.

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Internal Components page 14

Power

Entry

Power

Switch

Fuse

Network Connector

NOTES: When you’re using a 96-well plate, well A1 should be in the top left corner of the insert.

You can only use V-bottom plates with the 96-well plate insert.

Remove plate lids before inserting a 96-well plate into Maurice.

You can find info on where to load reagents and samples for cIEF applications in “Step 4: Load Samples and Reagents” on page 115 and for CE-SDS applications in “Step 4: Load Samples and Reagents” on page 157.

Rear Panel

Located on Maurice’s rear panel is the power entry, power switch and network connector.

• System Power - The main system power components consist of the power input, fuse and power switch

!WARNING!

Only use the power supply cord provided with Maurice. If the cord is damaged, please contact ProteinSimple Technical Support.

!WARNING! You can't replace or service any parts on Maurice except the power entry fuse.

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Internal Components page 15

!WARNING! SHOCK HAZARD

Disconnect the power cord from Maurice’s power input to disconnect power to the instrument.

• Network connection - A 10/100/1000 Mbps Ethernet (RJ-45 connector) is used to connect Maurice to a computer or local network.

NOTE: Serial numbers are used to identify individual instruments.

System Labels

A full system label is located on the rear panel. It includes the ProteinSimple location, system model, power requirements, serial number and certification markings.

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Computer Workstation page 16

A serial number label is located on the Maurice system’s front lower right side, on the silver system base.

Computer Workstation

The PC has two built-in Ethernet ports, one is used for Maurice and the other is available for your company’s network. ProteinSimple configures one port to have a fixed IP for a local link connection to the instrument, the other is configurable by users and will typically use a DHCP for dynamic IP.

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Computer Workstation page 17

User Guide for Maurice, Maurice C. and Maurice S.

Chapter 4:

Compass for iCE Overview

Chapter Overview

• Launching Compass for iCE

• Compass for iCE Overview

page 18

•Software Menus

• Changing the Compass for iCE Main Window Layout

• Software Help

• Checking for and Installing New Versions of Compass for iCE

•Viewing Release Notes

• Viewing the Software Log

• Compass for iCE Version Information

• Directory and File Information

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Instrument

Status Bar

Screen Panes

Title bar

Screen Tabs

Status Bar

Main Menu

Launching Compass for iCE

To open Compass for iCE, just double-click the icon on the computer desktop.

Compass for iCE Overview

Compass for iCE has three main screens:

• Batch - You’ll create and review your batch.

• Run Summary - Check out the status of your run.

• Analysis - Take a look at the data from your experiment.

Each screen has these components:

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Compass for iCE Overview page 20

Changing the Screen View

To toggle between the Batch, Run Summary and Analysis screens, just click the button in the screen tab located in the upper right corner of the main window.

Batch Screen

The Batch screen is used to create, view, and edit batches. You can assign samples to 96-well plate wells or vials, create and modify methods, customize your injection list and assign methods to each of your injec tions.

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Run Summary Screen

The Run Summary screen is used to monitor status of a batch in progress, the CE-SDS separation or cIEF Focus series for each injection and the current and voltage plots for each injection.

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Analysis Screen

The Analysis screen is used to view data from your batch, including the graph view (electropherograms) and a table with your results. You can also analyze your data for completed runs.

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Compass for iCE Overview page 24

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page 25 Chapter 4: Compass for iCE Overview

Screen Panes

Each of the Batch, Run Summary and Analysis screens have multiple panes that let you view the individual components of a batch, method or data file. Each pane has a labeled tab and a unique icon. We'll describe panes specific to each screen later in the individual screen sections.

The active pane in a screen is blue. To view a pane, click in the pane or on its tab. The example below shows panes in the Batch screen, and the Graph pane is active:

Title Bar

In the title bar you will see the batch file name and the icons that allow the main Compass for iCE window to be minimized, maximized or closed.

Main Menu

Access to various software, instrument and screen operations is available through the main menu. More details on menu commands can be found in

“Software Menus” on page 26.

Instrument Status Bar

The instrument status bar is used to start batches and cleaning protocols, indicate system status and show run progress. More details on instrument control and status can be found in Maurice C. and Maurice S.“.

Chapter 10, “Controlling Maurice,

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Software Menus page 26

NOTE: You will only see the instrument status bar when Compass for iCE is connected to an instrument. There is no status bar on computer workstations that you're only using for data analysis.

Screen Tab

The screen tab lets you move between Batch, Run Summary or Analysis screens and is located in the upper right corner of the main window. Just click a button to view a screen.

View Bar

The view bar is only displayed in the Analysis screen as part of the main menu, and allows you to switch between viewing standards or sample data, data for a single injection or all injections in the batch, or grouped injection data. View bar options are in page 251 for CE-SDS applications.

“Viewing Run Data” on page 346 for cIEF applications or

Compass for iCE Status Bar

The status bar is in the lower right corner of the main window. It displays active software processes and their progress.

Software Menus

Some of the items in the Compass for iCE main menu are available in specific screens only, and menu commands change depending on which screen is active. You can find menus and commands available for each screen in the Data Analysis“ and Chapter 11, “CE-SDS Data Analysis“.

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Chapter 5, “cIEF Batches“, Chapter 6, “CE-SDS Batches“, Chapter 9, “Run Status“, Chapter 12, “c IEF

page 27 Chapter 4: Compass for iCE Overview

File Menu

The File menu contains basic file commands.

Edit Menu

The Edit menu contains basic editing commands, analysis and preferences options. Specific details on preferences are described in Chapter 13, “Setting Your Preferences“.

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Software Menus page 28

View Menu

The View menu is only available in the Analysis screen, and allows you to change how your data is displayed. For more info on view options check out for CE-SDS applications, and “Using Groups” on page 357 for cIEF applications or page 262 for CE-SDS applications.

“Viewing Run Data” on page 346 for cIEF applications or page 251

Instrument Menu

The Instrument menu is only available when the software is connected directly to your instrument. You can learn more about instrument control options in

Chapter 10, “Controlling Maurice, Maurice C. and Maurice S.“

Window Menu

The Window menu lets you to switch between the Batch, Run Summary or Analysis screens, and restore screens to the default layout.

• Batch - Displays the Batch screen where you create, view, and edit batches.

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• Run Summary - Displays the Run Summary screen which lets you view the status of a batch in progress.

• Analysis - Displays the Analysis screen that lets you view electropherograms and results and change analysis parameters

• Default Layout - Restores the individual panes in the current screen back to their default size and location.

Help Menu

The Help menu gives you access to Help, software updates, release notes and other software info.

• User Guide - Displays the User Guide for Maurice, Maurice C. and Maurice S.

• Check for Updates - Automatically checks to see if a new version of Compass for iCE is available.

• Release Notes - Displays the software release notes for the current and prior versions.

• Compass for iCE Log - Displays the software log file.

• About Compass for iCE - Displays the software version and build information.

Changing the Compass for iCE Main Window Layout

You can easily resize the main window and the individual panes in each screen. Screen panes can also be moved outside of the main window.

Resizing the Main Compass for iCE Window

To resize the main window, roll the mouse over a corner or border until the sizing arrow appears. Then just click and drag to resize.

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Changing the Compass for iCE Main Window Layout page 30

Resizing the Screen Tab

The screen tab can be sized to show all or just some of the screen buttons. To resize, roll the mouse over the left edge of the tab until the sizing arrow appears, then click and drag to resize. If a screen button is hidden, a double arrow will display in the tab. Just click to display and select the hidden screen.

Resizing Screen Panes

• To resize a pa n e - Roll the mouse over the pane border until the sizing arrow appears. Then just click and drag to resize.

• To maximize a pane - Click the maximize button in the upper right corner or double-click the tab.

The other panes in the screen will automatically minimize to pane bars in the task area along the window border.

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• To restore all minimized panes - Click Restore on the minimized pane bar.

• To restore only one minimized pane - Click the pane icon on the minimized pane bar.

• To restore a maximized pane to its original size - Double-click the tab or right click the tab and click Restore.

• To restore all panes to their original sizes - Select Window in the main menu and click Default

Layout.

Changing the Location of Screen Panes

Panes can be moved to different locations within a screen.

• To move a pane - Click on its tab and drag it to the new location. As the pane is moved, area guides will display to assist you in choosing a drop location.

Area guides with a black arrow let you know that if the pane is dropped at that location, it will be resized and relocated as an individual pane in that area of the screen.

Area guides with a folder let you know that if the pane is dropped at that location, it will be added as a new tab in an area with one or more pane tabs.

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Changing the Compass for iCE Main Window Layout page 32

Area guides with a window let you know that if the pane is dropped at that location, it will be a separate window outside the Compass for iCE main window.

This example shows the Analysis screen after moving the Graph pane:

• To detach a pane from the main window - Click on its tab and drag it outside the main Compass for iCE window or right click the tab and click Detached.

• To move a detached pane back inside the main window - Right click the tab and deselect

Detached.

• To restore all panes to their original locations - Select Window in the main menu and click

Default Layout.

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Restoring the Main Window to the Default Layout

To restore screen pane sizes and locations to the original Compass for iCE layout, select Window from the main menu and click Default Layout.

Software Help

Select Help and click User Guide to view Maurice Systems User Guide.

Checking for and Installing New Versions of Compass for iCE

The software can automatically check to see if a newer version of software is available. To do this:

1. Make sure the computer being used has an active internet connection.

2. Select Help and click Check for Updates. If an update is found, a screen will display with the new version that’s available.

3. Click Finish to start the download and install the update.

4. Follow the on-screen instructions to complete the software installation.

5. Reboot the computer before using the new version of software.

Viewing Release Notes

Select Help and click Release Notes to view a PDF with feature updates and bug fixes for new and past ver- sions of Compass for iCE. We recommend you review these notes whenever a software update is installed.

NOTE: You can contact ProteinSimple Technical Support to request the release notes for new versions of Compass for iCE before you install it.

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Viewing the Software Log page 34

Viewing the Software Log

Select Help and click Compass Log to view the software log file.

Compass for iCE Version Information

Select Help and click About Compass for iCE to view the software version and build number information.

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Directory and File Information

The main Compass for iCE directory is located in the Program Files folder, and also contains PDF files of the User Guide for Maurice, Maurice C. and Maurice S.

Batch and run files are located in the Documents folder in the User directory on your computer:

• Batches Folder - Contains all batch files that you’ve saved.

• New Batches Folder - Contains Maurice batch template files.

• Runs Folder - Contains all batch data files. Data is automatically written to this folder.

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Directory and File Information page 36

NOTE: When a Compass for iCE software update is performed, the template s in the New Batch folder are overwritten. If you have customized these batches, we recommend saving them in a unique subfolder prior to updating the software, then transferring them back to the New Batch folder after the update to avoid losing your customizations.

File Types

These file types are used by Compass for iCE:

• Batch Files - Use a *.batch file extension.

• Run Files - Use a *.mbz file extension. The default file format for run files is Date_Time_BatchName. An example run file name would be 2016-01-28_18-50-53_CE-SDS.mbz.

• Analysis Settings Files - Exported analysis settings files use a *.settings file extension.

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User Guide for Maurice, Maurice C. and Maurice S.

Chapter 5:

cIEF Batches

Chapter Overview

• Batch Screen Overview

• Opening a Batch

page 38

• Creating a New Batch

• Viewing Replicate Injections

•On-board Mixing

•Batch History

• Making Changes to a Batch

• Viewing and Editing Batches in Completed Runs

• Copying and Pasting Injection Names or Sample IDs from other Documents

• Importing and Exporting Methods

•Batch Reports

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Batch Screen Overview

You can use the Batch screen to create, view, and edit batches. To get to this screen, click the Batch screen tab:

Batch Screen Panes

The Batch screen has five panes:

• Layout - Displays a map of either the 96-well plate or 48-vial tray of batch sample locations.

• Injections - Lists the injections, sample ID, sample locations and methods that Maurice or Maurice C. will execute for each sample in the batch.

• History - Lists all batch file events from initial creation to the most current update.

• Notes - Lets you enter specific information about your batch.

• Methods - Lets you create methods and enter method parameters used in the batch.

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Batch Screen Overview page 40

Software Menus Active in the Batch Screen

These main menu items are active in the Batch screen:

•File

•Edit

• Instrument (when the software is connected to Maurice or Maurice S.)

•Window

•Help

File Menu

These File menu options are active:

• New Batch - Creates a new batch from a starter template.

• Open Batch - Opens an existing batch.

• Save/Save As - Saves the open batch.

• Export Methods - Exports method(s) from the current batch as separate files.

• Import Methods - Imports saved method(s) into the current batch.

• Batch Report - Exports a table of sample and method details for each injection in the batch as a PDF file.

• Exit - Closes Compass for iCE.

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Edit Menu

The following Edit menu options are active in the Batch screen:

• Cut - Cuts the information currently selected.

• Copy - Copies the information currently selected.

• Paste - Pastes the copied information.

NOTE: Cut, Copy and Paste work differently depending on the pane you’re in. How and when you can use them is described throughout the chapter.

• Plate Layout - Lets you select between a 96-well plate or a 48-vial tray to run samples in.

• Default Analysis - Displays the default settings that will be used to analyze the data generated with your batch.

• Preferences - Lets you set and save your preferences for Access Control, data export, graph colors, grouped data and Twitter settings. See Chapter 13, “Setting Your Preferences“ for more information.

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Opening a Batch page 42

Opening a Batch

To open an existing batch:

1. Select File in the main menu and click Open Batch.

2. A list of the last five batches opened will display. Select one of those or click Browse to open the Batches folder and select a different one.

3. To make changes to the batch, see the steps in “Creating a New Batch” on page 43. When you’re done, select File from the main menu and click Save.

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Creating a New Batch

To create a new batch, we recommend that you use one of the template methods and make any necessary modifications from there.

Step 1 - Open a Template Batch

1. Select File in the main menu and click New Batch:

NOTE: If you’re using a Maurice system, both cIEF and CE-SDS template batches are available in the menu.

2. Select Maurice cIEF. A batch using the default method will display.

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Creating a New Batch page 44

Step 2 - Assign Your Samples

The Layout pane shows the default locations of where batch reagent should be placed in your Maurice system’s samples and reagents platform, and a map of either the 96-well plate or the 48-vial tray used for samples.

The same reagent locations are used for every batch:

• P1 - 0.5% Methyl Cellulose with blue pressure cap

• P2 - Fluorescence Calibration Standard with blue pressure cap

• P3 - Water vial with blue pressure cap

• P6 - Empty vial (air) with blue pressure cap

• N1 - Water vial with clear screw cap If you are using Maurice-OBM or Maurice C.-OBM to do on-board mixing:

• N2 - Water vial with clear screw cap

• N3- Empty vial with clear screw cap

• M1 - 6 mL IEF Separation Mix vial

• M2 - N/A for 48 samples or less, add a 6 mL IEF Separation Mix vial when running more than 48

samples

• M3 - 6 mL water vial

• M4 - 6 mL water vial

1. To assign samples, select 48 vials or a 96-well plate depending on what you’re running. Clicking on the vial/plate icon toggles between formats.

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2. To select samples:

• Add samples and select methods later: Use your mouse to highlight the well or vial positions your samples are located in, then click Add. For this example we’re using vials.

NOTE: The template batch automatically adds a sample in well or vial A1 by default, but if you don’t have a sample in this position you can remove it after you’ve added new positions for your samples.

• Add samples with preassigned methods: Highlight the well or vial positions your samples are located in, then right-click and select a method.

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Creating a New Batch page 46

Either option of adding samples populates the Injections table:

To remove samples, just highlight the ones you want to remove and click Remove. They’ll also be removed in the Injections table. You can also right-click well(s) or vial(s) in the Layout pane and click

Remove Sample(s).

3. Compass for iCE can monitor the current during a separation for you, stop it if the current drops below the minimum value and reinject the sample for you automatically. Reinjections are enabled by default, but if you don’t want to use this feature just click the reinject icon to toggle it off.

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NOTES: If a sample was reinjected, the injection row will be flagged in the Injections pane in the Run Summary screen. See “Injection Flags” on page 197 for more info.

A maximum of 10 reinjections are allowed per batch.

4. The sample platform is temperature controlled with a default setting of 10 °C. To change the temperature or turn it off, click the down arrow next to the temperature and select an option.

NOTE: The two reagent rows are kept at room temperature.

Step 3 - Assign Your Method Parameters

NOTE: We recommend using the default method parameters. Please see the Maurice cIEF Method Development Guide for more information on method optimization.

The Methods pane lists all of the methods used in a batch. You can add and remove methods here and change method parameters.

To import a saved method:

1. Select File in the main menu and click Import Method.

2. Select a method file (*.method) and click OK. The imported information will display in the Method pane.

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Creating a New Batch page 48

To create a new method or make changes to an existing one:

1. In the Methods table, click the first cell in the Name column and enter a new method name if needed.

2. Click the first cell in the Separation column, then click the selection button [...] to set the pre-focusing and focusing time (in minutes) and voltage (V).

• To change time and voltage parameters: Just click in a cell under Time or Voltage and type

the new value(s).

• To add a profile step: Click Add. A new row will be added in the table. Then just type in a load

time (in minutes) and voltage value (in V).

• To remove a profile step: Select the row you want to remove and click Remove.

3. Click the first cell in the Detection column the selection button [...] to set your exposure times for absorption and fluorescence detection modes.

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• To change the exposure time: Just click in a cell under Exposure and type the new value(s) in

seconds.

NOTES: The first exposure is an instrument default setting and can’t be changed.

Fluorescence is the default detection for the remaining exposures and can’t be changed.

• To add a profile step: Click Add. A new row will be added in the table. Then just type in an expo- sure time (in seconds).

• To remove a profile step: Select the row you want to remove and click Remove.

4. Click the first cell in the Sample Load(s) column and set the load time in seconds.

NOTE: We recommend using the default Sample Load time of 55 seconds. Please contact ProteinSimple Technical Support if you have questions on the Sample Load time to use for your application.

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Creating a New Batch page 50

5. Click the first cell in the pI Markers column to select pI markers. Add new markers or remove existing ones then click OK.

NOTE: When you edit the pI markers in the method for a batch, Compass for iCE automatically creates a Markers group in the pI Markers Analysis settings for you.

• To add a pI marker: Click Add. A new row will be added in the table. Then just type in a pI and a

position (in pixels).

• To remove a pI marker: Select the row you want to remove and click Remove.

6. Optional: Click the first cell in the Ampholytes column and enter the ampholytes you’re using.

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7. Optional: Click the first cell in the Additives column and enter any additives you’re using.

8. You can now:

•Click New in the upper right corner of the Methods pane to add a new one if you want to use dif- ferent methods for different samples. Then just repeat the steps above for the new method.

• Make updates to the remaining methods by repeating the prior steps.

• Select a method and click Remove in the upper right corner of the Methods pane to delete it.

Step 4 - Set Up Your Injections

The Injection pane lists all sample injections for the batch. Any samples that were added in “Step 2 - Assign Your Samples” are automatically added to this list.

Selecting an injection in the table also selects the sample the injection will be made from in the plate or vial map in the Layout pane and vice-versa.

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1. To add sample names, click the Sample ID cell for the injection and type a name.

NOTES: Sample IDs can be copied from other documents and pasted into Compass for iCE to make batch setup faster. Check out “Copying and Pasting Injection Names or Sample IDs from other Documents” on page 69 for more info.

If you change the sample ID when the default injection name is still being used, the injection name will automatically update to match the sample ID. If you’ve already changed the injection name manually, updating the sample ID will not update the injection name.

The sample name also displays when you hover the mouse over the sample in the plate or vial map:

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2. Injection names are automatically set to ‘Sample ID_injection number’. To change the name, click the

Injection Name cell for the injection and type a name. Each injection name must be unique. If they

aren’t when you save the batch, Compass for iCE highlights any non-unique injection names so you can change them.

NOTE: Changing the injection name won't affect the sample ID.

3. Assign your methods to each injection. Just click the Method cell for the injection and select a method from the drop down menu. If you added your samples with pre-assigned methods and don’t need to make any changes, skip to the next step.

Hovering over a method name displays the method parameters:

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Creating a New Batch page 54

4. You can add or remove injections as needed using a few different options. If you don’t need to make any changes here, just skip to the next step.

• To add sequential replicate injections: Highlight an injection and click Replicate. A new injec-

tion will be added under the row you selected

• To add injections at the end of the batch: Click on any sample position in the Layout pane or

sample injection in the Injection table and click Add. A new injection using the same sample will be added to the end of the table. Assign the method if needed.

• To copy and paste injections: Right-click on an injection in the Injection table and select Copy.

Click on the injection number above where you want to insert the copied injection, right click and select Paste. The copied injection will be inserted under the row you selected.

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Step 5 - Setup On-Board Mixing (Optional)

If you’re using Maurice-OBM or Maurice C.-OBM, you can tell Compass for iCE which samples to automatically mix for you. Up to 96 samples can be on-board mixed in a batch. You can also have a mix of premixed samples and samples to on-board mix in the same batch.

NOTE: To use the OBM function, you must load 25 μL of sample in every sample vial or plate well that will be mixed on board. Maurice will automatically mix 25 μL of each sample with 100 μL of IEF Separation Mix prior to injection.

1. Make sure you’ve placed the following on-board mixing reagents in Maurice:

• N2 - Water vial with clear screw cap

• N3 - Empty vial with clear screw cap

• M1 - 6 mL IEF Separation Mix vial

• M2 - 6 mL IEF Separation Mix vial (use only when running more than 48 samples)

• M3 - 6 mL water vial

• M4 - 6 mL water vial

2. The on-board mixing function is disabled by default. Click the on-board mixing icon to toggle it on.

All sample positions will now display the mixing icon, and the on-board mixing reagent vial positions will also display on the left:

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Creating a New Batch page 56

The Injection pane will also display a Mix Bottle column showing the position of the IEF Separation Mix vial that will be used for each sample. The vial in the M1 position is used for samples 1-48, and the vial in M2 will automatically display and be used for samples 49-96:

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3. To turn on-board mixing off for specific samples, select those well(s) or vial(s), right click and select Mix-

ing Off.

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Creating a New Batch page 58

The sample positions will display solid orange indicating mixing is off, and the Mix Bottle designation in the Injections pane is removed:

Step 6 - Add Programmed Pauses and Stops (Optional)

You can program pauses and stops in the batch to automatically pause or stop after injections even when you aren't in front of Maurice.

To program a p a u se :

Pauses are helpful when you want to review injection results before continuing with the rest of the batch.

1. Highlight the injection you want to pause at and click Pause. The batch will pause after that injection is complete.

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NOTE: Maurice can tweet you when the batch pauses. See “Setting Up Maurice Systems to Send Tweets” on page 440.

2. To resume the batch, click Continue in the instrument status bar:

To stop the run after a specific injection:

1. Highlight the injection you want the batch to stop at and click Stop. Maurice will continue the batch through the selected injection, then stop the batch and perform end-run cleanup steps.

Data collected for injections completed up to the point where the batch was stopped are saved in the .mbz file.

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Creating a New Batch page 60

2. Be sure to complete your normal post-batch procedures.

Step 7 - Add Batch Notes (Optional)

1. Click on the Notes pane.

2. Click in the notes area and type any information you want to add about your batch.

Step 8 - Modify Default Analysis Parameters (Optional)

You can preset the parameters used to analyze data generated with the batch. We recommend using the default parameters for cIEF applications, but if you need to modify parameters:

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1. Select Edit from the main menu and click Default Analysis. The following screen will display:

2. Change the parameters you want to, then click OK. For detailed information on analysis parameters, please refer to “Analysis Settings Overview” on page 386.

Step 9 - Save Your Batch

1. Once all of your sample, method and injection info is entered, select File > Save. Enter any comments on the batch if you want, then click Save.

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Viewing Replicate Injections page 62

2. Enter a name for your batch then click Save.

Viewing Replicate Injections

Injections with replicates have an arrow next to the injection number. You can expand or collapse the list of replicate injections by toggling the arrow:

• To show all replicate injections in the batch, click the Expand All Injections button.

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• To hide all replicate injections in the batch, click the Collapse All Injections button.

On-board Mixing

Maurice's On-board Mixing function (OBM) can be used to automate preparation of cIEF samples by adding and aspirating the ampholyte mix into your protein samples. OBM is really useful when you need to analyze proteins using platform methods and when your proteins have a limited stability in their sample solution. It also minimizes assay complexity while maximizing mixing accuracy and consistency.

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On-board Mixing page 64

You can use OBM with samples in both 96-well plates and in vials, and on up to 96 samples in a single batch. The mixing protocol uses a fixed set of aspiration volume, speeds, and cycle parameters. The OBM protocol adds 100 μL of IEF Separation Mix to 25 μL of sample so the IEF Separation Mix components should be 1.25 x that of the final desired concentration. Reagent positions M1-M4 on Maurice’s sample and reagent platform all hold 6 mL vials that are used for on-board mixing.

• M1 and M2: Used for the IEF Separation Mix that contain the ampholytes, methyl cellulose, and excipient components of a cIEF sample solution. M1 is used for samples 1-48 and M2 is used for samples 49-96. The minimum IEF Separation Mix vial volume is 1.5 mL, and the maximum is 6.0 mL.

• M3 and M4: Used for DI water which is used in both aspirating the IEF Separation Mix and cleaning the OBM pipette between sample preparation cycles. Place two full, fresh 6 mL vials of DI water in positions M3 and M4 prior to running an OBM batch.

The on-board mixing function is disabled by default. Click the on-board mixing icon to toggle it on.

All sample positions will now display the mixing icon, and the on-board mixing reagent vial positions will also display on the left:

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To turn on-board mixing off for specific samples, select those well(s) or vial(s), right click and select Mixing

Off.

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Batch History page 66

The sample positions will display solid orange indicating mixing is off, and the Mix Bottle designation in the Injections pane is removed:

Batch History

Clicking on the History pane shows the batch event history, starting with the date and time the batch was created through the most current event. Clicking on a row in the table displays the full event details in the box under the table.

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• Date: Date and time of the batch event.

• User Name: User that initiated the event. User names only display if you’re using the Access Control feature to help satisfy the 21CFR Part 11 data security requirements. Go to “Enabling Access Control” on page 458 to learn how to set it up.

• Message: Description of the event that took place.

• Comment: Comments entered by the user when the batch was saved.

You can copy the information in the History pane to use in other documents:

1. Click the History pane to make sure it’s active.

2. Click Edit in the main menu and select Copy.

3. Open a document and click Paste.

Making Changes to a Batch

1. Select File in the main menu and click Open Batch.

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Viewing and Editing Batches in Completed Runs page 68

2. A list of the last five batches opened will display.

• Select one of these files or click Browse to open the Batch folder and select a different one.

• To open a batch used in a run file, click Browse. Go to the Run folder in the Compass for iCE directory and select the run file that uses the batch you want to edit.

3. To make changes to the batch, see the steps in “Creating a New Batch” on page 43. Then select File from the main menu and click Save or Save As.

Viewing and Editing Batches in Completed Runs

1. Click the Analysis screen and open your run file(s).

2. After the run opens, click the Batch screen to view the batch used with the run.

If you opened more than one run file, you can switch between viewing batches for each file. Just click the down arrow in the Run box and select the batch you want to view from the drop down list.

3. In completed run files, you can only make edits to the sample and method names in a batch, and these changes are saved to the run file only. Update sample and method names as needed.

4. Click the Analysis screen. Then select File from the main menu and click Save or Save As to save the new changes to the run file.

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Copying and Pasting Injection Names or Sample IDs from other Documents

Injection names and Sample IDs can be copied from other documents and pasted into the Injection pane to make batch setup faster.

To paste a list of injection names into the batch:

1. Select the list of injection names in a document (Microsoft® Word®, Excel®, etc.) and select Copy or hold the Ctrl+C keys to copy.

2. Select an injection in the Injection pane, then hold the Ctrl+V keys to paste. You can also select the injection, right click and select Paste.

The injection names are pasted into the Injection pane:

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Copying and Pasting Injection Names or Sample IDs from other Documents page 70

To paste a list of Sample IDs into the batch:

1. Select the list of Sample IDs in a document (Microsoft Word, Excel, etc.) and select Copy or hold the

Ctrl+C keys to copy.

2. Select a Sample ID in the Injection pane, then hold the Ctrl+V keys to paste. You can also select the Sample ID, right click and select Paste.

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The Sample IDs are pasted into the Injection pane.

NOTE: If you paste in new sample IDs when the default injection names are still being used, the injection names will automatically update to match the sample ID. If you’ve already changed the injection names manually, updating the sample IDs will not update the injection names.

Importing and Exporting Methods

Methods in an open batch or run file can be exported as a separate file. This lets you import the same method into another batch later, rather than having to re-enter method information manually.

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Importing and Exporting Methods page 72

Importing a Method

NOTE: Importing a method imports information into the Batch window’s Method pane only.

1. Open the batch you want to import the method into.

2. Select File in the main menu and click Import Method.

3. Select a method file (*.method) and click OK. The imported information will display in the Method pane.

Exporting a Method

NOTE: Exporting a method exports information in the Batch window’s Method pane only.

1. Open the batch you want to export the method from.

2. In the Method pane, select the method you want to export. To export more than one method at the same time, hold the Ctrl key to select multiple methods.

3. Select File in the main menu and click Export Method. The following window displays:

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4. The default directory is Compass for iCE/Batches. Change the directory if needed.

5. Enter a method file name and click Save. The settings will be saved as a *.method file.

Batch Reports

You can export a PDF file of sample and method details for each injection in the batch.

NOTES: You can have Compass for iCE generate reports for you automatically at the end of each run. See “Setting up Automatic Injection Reports” on page 436 for more information.

Any time a new report is run, it’s saved in a separate run folder. Existing reports aren’t overwritten.

1. Go to the Analysis or Run Summary screen, then click File > Open Run and select a run file (if you don’t have one open already).

2. After the run opens, go to the Batch screen.

3. Select File from the main menu and click Batch Report.

4. The report name defaults to the run file name. If you want to change it, type in the Report Name box to make updates.

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Batch Reports page 74

cIEF Batch: Maurice cIEF

Injection Injection Name Sample ID Location Method Notes

1 System Suitability_01 System Suitability A1 System Suitablity

2 mAb 11 Blank_02 mAb 11 Blank A2 mAb Method

3 mAb 11 Ref. Std._03 mAb 11 Ref. Std. A3 mAb Method

4 mAb 11 Prep 20160121_04 mAb 11 Prep 20160121 A4 mAb Method

5 mAb 11 Prep 20160121_05 mAb 11 Prep 20160121 A4 mAb Method

6 mAb 11 Prep 20160121_06 mAb 11 Prep 20160121 A4 mAb Method

7 mAb 11 Ref. Std._07 mAb 11 Ref. Std. A3 mAb Method

8 mAb 11 Blank_08 mAb 11 Blank A2 mAb Method

Injections

Methods

Name Separation Detection Sample Load (s) pI Markers Ampholytes Additives

System Suitablity 1.0 min, 1500 Volts

4.5 min, 3000 Volts

absorbance, 0.005 sec fluorescence, 3 sec fluorescence, 5 sec fluorescence, 10 sec fluorescence, 20 sec

90 3.38 pI, 250 pixels

10.17 pI, 1700 pixels

mAb Method 1.0 min, 1500 Volts

6.0 min, 3000 Volts

absorbance, 0.005 sec fluorescence, 3 sec fluorescence, 5 sec fluorescence, 10 sec fluorescence, 20 sec

90 4.05 pI, 500 pixels

9.99 pI, 1800 pixels

Batch Log

Date User Name Message Comment

01/21/2016 9:38 AM Batch created using the factory default Maurice cIEF

Created By: Jacquelyn Sun 10:37 PM Jun 11, 2017 CDT

C:\Users\Jacquelyn\Documents\Compass for iCE\Runs\2016-01-21_09-46-39_mAb11_Prep20160121_QC(0).mbz Computer: DESKTOP-C7FPQGB

Page 1 of 2

Software Version:

2.0.8, Build ID: 0606

5. The Batch Report PDF is exported to the Runs folder in the Compass for iCE directory. It’ll be in a folder with the report name used in the prior step. When the reports are done, the folder opens for you automatically.

Here’s an example Batch Report:

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cIEF Batch: Maurice cIEF

Date User Name Message Comment

01/21/2016 9:47 AM Save protocol and template changes Auto-saved

Created By: Jacquelyn Sun 10:37 PM Jun 11, 2017 CDT

C:\Users\Jacquelyn\Documents\Compass for iCE\Runs\2016-01-21_09-46-39_mAb11_Prep20160121_QC(0).mbz Computer: DESKTOP-C7FPQGB

Page 2 of 2

Software Version:

2.0.8, Build ID: 0606

User Guide for Maurice, Maurice C. and Maurice S.

Chapter 6:

CE-SDS Batches

Chapter Overview

• Batch Screen Overview

• Opening a Batch

page 76

• Creating a New Batch

• Viewing Replicate Injections

•Batch History

• Making Changes to a Batch

• Viewing and Editing Batches in Completed Runs

• Copying and Pasting Injection Names or Sample IDs from other Documents

• Importing and Exporting Methods

•Batch Reports

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Batch Screen Overview

You can use the Batch screen to create, view, and edit batches. To get to this screen, click the Batch screen tab:

Batch Screen Panes

The Batch screen has five panes:

• Layout - Displays a map of either the 96-well plate or 48-vial tray of batch sample locations.

• Injections - Lists the injections, sample ID, sample locations and methods that Maurice or Maurice S. will execute for each sample in the batch.

• History - Lists all batch file events from initial creation to the most current update.

• Notes - Lets you enter specific information about your batch.

• Methods - Lets you create methods and enter method parameters used in the batch.

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Batch Screen Overview page 78

Software Menus Active in the Batch Screen

These main menu items are active in the Batch screen:

•File

•Edit

• Instrument (when the software is connected to Maurice or Maurice S.)

•Window

•Help

File Menu

These File menu options are active:

• New Batch - Creates a new batch from a starter template.

• Open Batch - Opens an existing batch.

• Save/Save As - Saves the open batch.

• Export Methods - Exports method(s) from the current batch as separate files.

• Import Methods - Imports saved method(s) into the current batch.

• Batch Report - Exports a table of sample and method details for each injection in the batch as a PDF file.

• Exit - Closes Compass for iCE.

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Edit Menu

The following Edit menu options are active in the Batch screen:

• Cut - Cuts the information currently selected.

• Copy - Copies the information currently selected.

• Paste - Pastes the copied information.

NOTE: Cut, Copy and Paste work differently depending on the pane you’re in. How and when you can use them is described throughout the chapter.

• Plate Layout - Lets you select between a 96-well plate or a 48-vial tray to run samples in.

• Default Analysis - Displays the default settings that will be used to analyze the data generated with your batch.

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Opening a Batch page 80

Opening a Batch

To open an existing batch:

1. Select File in the main menu and click Open Batch.

2. A list of the last five batches opened will display. Select one of those or click Browse to open the Batches folder and select a different one.

3. To make changes to the batch, see the steps in “Creating a New Batch” on page 81. When you’re done, select File from the main menu and click Save.

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Creating a New Batch

To create a new batch, we recommend that you use one of the template methods and make any necessary modifications from there.

Step 1 - Open a Template Batch

1. Select File in the main menu and click New Batch:

NOTE: If you’re using a Maurice system, both cIEF and CE-SDS template batches are available in the menu.

2. Select Maurice CE-SDS. A batch using the default method will display.

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Creating a New Batch page 82

Step 2 - Assign Your Samples

The same reagent locations are used for every batch:

• P1 - Conditioning Solution 1 with orange pressure cap

• P2 - Conditioning Solution 2 with orange pressure cap

• P3 - DI water with orange pressure cap

• P4 - Separation Matrix with orange pressure cap

• P5 - Wash Solution vial with orange pressure cap

• P6 - Empty vial (air) with orange pressure cap

• N1 - Wash Solution vial with clear screw cap

• N2 - Wash Solution vial with clear screw cap

• N4 - Running Buffer - Bottom with clear screw cap

1. To assign samples, select a 96-well plate or 48 vials depending on what you’re running. Clicking on the vial/plate icon toggles between formats.

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2. To select samples:

• Add samples and select methods later: Use your mouse to highlight the well or vial positions your samples are located in, then click Add. For this example we’re using a 96-well plate.

• Add samples with preassigned methods: Highlight the well or vial positions your samples are located in, then right-click and select a method.

Either option of adding samples populates the Injections table:

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Creating a New Batch page 84

Remove Sample(s).

NOTES: If a sample was reinjected, the injection row will be flagged in the Injections pane in the Run Summary screen. See “Injection Flags” on page 197 for more info.

A maximum of 10 reinjections are allowed per batch.

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4. The sample platform is temperature controlled with a default setting of 10 °C. To change the temperature or turn it off, click the down arrow next to the temperature and select an option.

NOTE: The two reagent rows are kept at room temperature.

Step 3 - Assign Your Method Parameters

NOTE: There are three default methods. We recommend using the default method parameters for the listed sample types. Please see the Maurice CE-SDS Application Guide for more information on method optimization.

The Methods pane lists all of the methods used in a batch. You can add and remove methods here and change method parameters.

To import a saved method:

1. Select File in the main menu and click Import Method.

2. Select a method file (*.method) and click OK. The imported information will display in the Method pane.

To create a new method or make changes to an existing one:

1. In the Methods table, click the first cell in the Name column and enter a new method name if needed.

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Creating a New Batch page 86

2. Click the first cell in the Sample Load column, then click the selection button [...] to set your sample load profile time (in seconds) and voltage.

• To change time and voltage parameters: Just click in a cell under Time or Voltage and type

the new value(s).

• To add a profile step: Click Add. A new row will be added in the table. Then just type in a load

time (in seconds) and voltage value.

• To remove a profile step: Select the row you want to remove and click Remove.

3. Click the first cell in the Separation column the selection button [...] to set your separation profile parameters (in minutes) and voltage.

NOTE: Run your reduced IgG samples and IgG Standard for 25 minutes and the CE-SDS MW Markers for 30 minutes. Run your non-reduced IgG samples and IgG Standard for 35 minutes. The default separation voltage for all sample types is 5750 volts.

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• To change time and voltage parameters: Just click in a cell under Time or Voltage and type

the new value(s).

• To add a profile step: Click Add. A new row will be added in the table. Then just type in a load time (in seconds) and voltage value.

• To remove a profile step: Select the row you want to remove and click Remove.

4. You can now:

•Click New in the upper right corner of the Methods pane to add a new one if you want to use dif- ferent methods for different samples. Then just repeat the steps above for the new method.

• Make updates to the remaining methods by repeating the prior steps.

• Select a method and click Remove in the upper right corner of the Methods pane to delete it.

Step 4 - Set Up Your Injections

The Injection pane lists all sample injections for the batch. Any samples that were added in “Step 2 - Assign Your Samples” are automatically added to this list.

Selecting an injection in the table also selects the sample the injection will be made from in the plate or vial map in the Layout pane and vice-versa.

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Creating a New Batch page 88

1. To add sample names, click the Sample ID cell for the injection and type a name.

The sample name also displays when you hover the mouse over the sample in the plate or vial map:

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2. Injection names are automatically set to ‘Sample ID_injection number’. To change the name, click the

Injection Name cell for the injection and type a name. Each injection name must be unique. If they

aren’t when you save the batch, Compass for iCE highlights any non-unique injection names so you can change them.

NOTE: Changing the injection name won't affect the sample ID.

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Creating a New Batch page 90

Hovering over a method name displays the method parameters:

4. You can add or remove injections as needed using a few different options. If you don’t need to make any changes here, just skip to the next step.

• To add sequential replicate injections: Highlight an injection and click Replicate. A new injec-

tion will be added under the row you selected

• To add injections at the end of the batch: Click on any sample position in the Layout pane or

sample injection in the Injection table and click Add. A new injection using the same sample will be added to the end of the table. Assign the method if needed.

• To copy and paste injections: Right-click on an injection in the Injection table and select Copy.

Click on the injection number above where you want to insert the copied injection, right click and select Paste. The copied injection will be inserted under the row you selected.

User Guide for Maurice, Maurice C. and Maurice S.

ProteinSimple Maurice S, Maurice C, Maurice User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

User Guide for Maurice, Maurice C. and Maurice S.

Table of Contents

Welcome

Maurice Systems

Introduction

Space Requirements

Physical Specifications

Electrical Requirements

Environmental Requirements

Software and Computer Requirements

General Guidelines and Information

Intended Use

Lifting and Moving the System: Lift Maurice Correctly

Maurice Systems

System Door

External Components

Status Light

Internal Components

Cartridge Slot

Sample and Reagent Platform

Rear Panel

System Labels

Computer Workstation

Launching Compass for iCE

Compass for iCE Overview

Changing the Screen View

Batch Screen

Run Summary Screen

Analysis Screen

Screen Panes

Title Bar

Main Menu

Instrument Status Bar

Screen Tab

View Bar

Compass for iCE Status Bar

Software Menus

File Menu

Edit Menu

View Menu

Instrument Menu

Window Menu

Help Menu

Changing the Compass for iCE Main Window Layout

Resizing the Main Compass for iCE Window

Resizing the Screen Tab

Resizing Screen Panes

Changing the Location of Screen Panes

Restoring the Main Window to the Default Layout

Software Help

Checking for and Installing New Versions of Compass for iCE

Viewing Release Notes

Viewing the Software Log

Compass for iCE Version Information

Directory and File Information

File Types

Batch Screen Overview

Batch Screen Panes

Software Menus Active in the Batch Screen

Opening a Batch

Creating a New Batch

Step 1 - Open a Template Batch

Step 2 - Assign Your Samples

Step 3 - Assign Your Method Parameters

Step 4 - Set Up Your Injections

Step 5 - Setup On-Board Mixing (Optional)

Step 6 - Add Programmed Pauses and Stops (Optional)

Step 7 - Add Batch Notes (Optional)

Step 8 - Modify Default Analysis Parameters (Optional)

Step 9 - Save Your Batch

Viewing Replicate Injections

On-board Mixing

Batch History

Making Changes to a Batch

Viewing and Editing Batches in Completed Runs

Copying and Pasting Injection Names or Sample IDs from other Documents