Promega GloMax-Multi Detection System Quick Start Manual

1.

RS-232

USB

Injector system

Power

On/Off

Unpacking the

instrument

GloMax®-Multi+ Detection
System with Instinct

Setting Up Your Instrument. It’s Easy!

For the complete protocol see Technical Manual, TM340, on the CD provided with your instrument or go to:

www.promega.com/protocols and search for TM340.

CAUTION! The GloMax

two people.

1. Set the shipping box on a table, and remove the accessories and literature.

2. Carefully remove the GloMax®-Multi+ Detection System from the box and place on a flat, level, solid surface in a
dust-free location with good air circulation. Leave at least 7.5 inches (19cm) of clearance in front of the instrument to
allow the instrument door to open without hindrance.

3. Install the Instinct Software™ on a PC/Windows

®

-Multi+ Detection System weighs approximately 35lb (16kg) and should be handled by

™

Software

®

computer.

2.

What’s in

the box?

3.

Setting

up the

instrument

4.

Starting up

the instrument

B.

A.

C.

D.

1. Open the accessories box included with your shipment,
and locate the instrument power cable.

2. Connect the AC Power Adapter to the power inlet on the
back of the Instrument. Connect the power cord to the
AC Power Adapter.

3. Plug the power cord into a grounded outlet.

1. Turn On the GloMax®-Multi+ Detection System. The power switch is located

2. The instrument will initialize and calibrate for about one minute, at which point

3. In order to prevent damage to the instrument and loss of sample it is important

8785TA

96 Well

Remove

Important: When using the instrument for the first time, you must press the

“Door” button and remove the red labels attaching the Microplate Adapter to the
Microplate Sample Tray.

E.

8066TA

on the back panel next to the power connection. An orange LED located left
of the touch screen will illuminate.

the touch screen will activate and the Home screen will appear.

to have the Microplate Adapter in the correct orientation. Please refer to
Technical Manual TM340 for complete instructions.

The GloMax
Software Accessories:

A. Power Cord. B. AC Power Adapter. C. Microplate Adapter,
located inside the instrument. D. Two Optical Well Crosstalk Masks,
one for 96-well plates and one for 384-well plates. The 96-well Optical
Crosstalk Mask will be located inside the instrument. E. USB Flash
Drive (appearance may vary from Figure E).

Included but not shown: CD containing GloMax
System Technical Manual (TM340) and Instinct software, one
spare red Breakaway Tab for the plate mechanism and a small
screwdriver.

Check to make sure all accessories are included with
the instrument and are undamaged.

®

-Multi+ Detection System with Instinct

®

serial port

port

connection

inlet

-Multi+ Detection

switch

6998TB

©2011 Promega Corporation. All Rights Reserved. Printed in USA 12/11. 18284-PM-CR. Part# FB117

6-48 Well

8007MA

Please refer to Technical Manual TM340 for detailed instructions on your
specific application.

Continued on other side.

5.

8009MA

AF9FB117 1211FB117

Setting

the read

parameters

6.

Starting a run

GloMax®-Multi+ Detection System
with Instinct

From the Home screen:

• For a wizard-guided protocol setup, select the “New Protocol” button.

• Alternatively, select a preprogrammed/saved protocol by selecting the “Select Protocol” button.

• Or, select the “Instrument Control” button to set new parameters.

• Select “Help Topics” to view all help topics.

Please refer to Technical Manual TM340 for Touch Screen Basics.

1. Touch the “Plate Mapping” button and highlight the wells to be read (green is selected and gray is deselected).
For information about using the built-in curve fitting and well labeling features of the Instinct software see Technical
Manual TM340.

2. Touch the “Door” button to open the instrument. Place the microplate in the sample tray. Make sure the
A1 well is at the top right corner.

3. If installed, do not use force or push on the Microplate Sample Tray Cover to close it.
The cover will close automatically.

4. Touch the “Start” button.

A1

5. Touch the “Edit” button, and use the keypad to enter a file name.

6. Touch the “OK” button to start reading.

Important: To prevent evaporation and damage to the detector head, always remove the microplate
when finished reading.

™

Software

7.

Results

8.

Helpful

resources

To view Results:

1. From the Instrument Control screen, touch the “Results” button.

2. Touch the button, and choose a saved file to view.

To Transfer Results File(s):

1. Insert a USB flash drive into the USB port on the front of the instrument to the left of the touch screen.

2. Touch the button on the upper right corner of the screen.

3. Touch the “OK” button to export only the selected files, or touch the “Copy all Files” button to export all displayed
and undisplayed results files.

4. To copy more than one file, touch the “Files” button, touch the Multi Select button and highlight multiple files to copy.

5. Touch the “USB” button to save to USB.

6. Move the USB flash drive to a PC/Windows
files (see Technical Manual TM340 for more information about viewing the results).

Important: Only the 50 most recent results files are displayed on the touch screen. All results files are stored
on the internal memory of the instrument. To ensure that data is saved, save the results files to a USB flash
drive frequently.

Detailed instructions on setting up and operating your GloMax
Manual TM340, included on the CD that shipped with your instrument and available at:

www.promega.com/protocols Helpful sections of TM340 include:

• Touch Screen Basics

Control

®

-Multi+ Detection System with Shaking

®

-Multi+ Detection System with Temperature

• GloMax

• GloMax

• Luminometer Operation

• Visible Absorbance Photometer Operation

8957TA

8956TA

®

computer where the Instinct software has been installed to download

®

-Multi+ Detection System can be found in Technical

• Fluorometer Operation

• Switching Fluorescent Optical Kits

• Installing Injectors

• Removing and Installing the Microplate Sample

Tray Cover

• Troubleshooting

• UV-Vis Absorbance Photometer Operation, Section 12

©2011 Promega Corporation. All Rights Reserved. Printed in USA 12/11. 18284-PM-CR. Part# FB117

• Fluorometer Operation, Section 13