Promega GloMax GM2000, GloMax GM2010 Operating Manual

OPERATING MANUAL

GloMax® Navigator System

Instrucons for Use of Products GM2000 and GM2010

4/16
TM470

GloMax® Navigator System

Visit the web site to verify that you are using the most current version of this Technical Manual.

E-mail Promega Technical Services if you have questions on use of this system: [e-mail]

All technical literature is available at: www.promega.com/protocols/

1. Introduction ................................................................................................................................ 2

1.A. Description ......................................................................................................................... 2

1.B. Specifications ...................................................................................................................... 3

1.C Product Components ........................................................................................................... 5

1.D. Precautions and Special Instructions ..................................................................................... 6

1.E. Environmental Requirements ............................................................................................... 8

1.F. Inspection and Unpacking .................................................................................................... 9

2. Setting Up the GloMax

2.A. Setting Up the GloMax

2.B. Switching On the GloMax

2.C. Shutting Down the GloMax

2.D. Operating the GloMax

®

3. GloMax

Navigator User Interface Screens .................................................................................. 14

®

Navigator System ................................................................................... 10

®

Navigator Instrument and Tablet PC ................................................ 10

®

Navigator Instrument................................................................. 11

®

Navigator Instrument .............................................................. 12

®

Navigator System ............................................................................ 12

3.A. Tablet PC Home Screen ...................................................................................................... 14

3.B. Quick Read Screen ............................................................................................................. 15

3.C. Protocol Screen ................................................................................................................. 16

3.D. Results Screen ................................................................................................................... 26

3.E. Settings Screen .................................................................................................................. 30

4. Operating the Injector System (optional) ..................................................................................... 36

4.A. Priming Injectors .............................................................................................................. 37

4.B. Reverse Purge (Retrieving Unused Reagents from Injectors, optional) ................................... 38

4.C. Flushing the Injectors after Use .......................................................................................... 38

5. Cleaning and Maintenance .......................................................................................................... 39

5.A. General Instrument Care .................................................................................................... 39

5.B. General Cleaning ............................................................................................................... 39

5.C. Tablet PC Care .................................................................................................................. 39

5.D. Cleaning the Optical Head .................................................................................................. 40

5.E. Cleaning the Instrument Interior ........................................................................................ 42

5.F. Cleaning the Injectors ........................................................................................................ 43

5.G. Cleaning the Waste Collection Tray ..................................................................................... 43

5.H. Removing or Replacing Inlet and Outlet Tubing ................................................................... 43

5.I. Additional Maintenance ..................................................................................................... 44

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6. Troubleshooting......................................................................................................................... 45

6.A. Error Messages ................................................................................................................. 45

6.B. Common Problems ............................................................................................................ 46

6.C. Use of a USB Flash Drive .................................................................................................... 48

7. Appendix ................................................................................................................................... 49

7.A. Configuring the Tablet PC ................................................................................................... 49

7.B. Icons and Symbols ............................................................................................................. 54

7.C. Warranty and Service ......................................................................................................... 55

7.D. Certificate of Decontamination ........................................................................................... 56

7.E. Related Products ............................................................................................................... 57

1. Introduction

1.A. Description

The GloMax

®

Navigator System

(a)

is an easy-to-use microplate luminometer integrated with Promega chemistries
for superior assay performance. This instrument provides researchers superior luminescence sensitivity and
dynamic range for both strong and weak bioluminescence experimental samples as well as seamless integration
with Promega industry-leading bioluminescence assays, including bioluminescent gene reporter, cell-based and
biochemical assays. The luminometer features optional dual injectors to measure both glow- and flash-based
luminescent reactions in 96-well plates.

The instrument is operated by an integrated Tablet PC, which provides quick and easy navigation through the
control options. Exporting your results is simple with a variety of options, including export to your local data
network, USB flash drive and cloud-based storage location. The GloMax

®

Navigator software provides many of
the required technical elements of a part 11-compliant system (user authentication and authorization, data
integrity and protection, electronic signatures and audit trails) when used with the appropriate laboratory
workflow.

Figure 1. The GloMax® Navigator System.

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1.B. Specifications

Instrument Specifications

Detection Modes Luminescence

Read Type Glow, Flash, Kinetic, Repeat

Sample Format 96-well plates

®

Tablet PC Windows

8 Tablet PC with USB connection to instrument,
wireless connectivity to local network; export to network, cloud or
USB flash drive

Dimensions 18.6in (47.2cm) width

20.0in (50.8cm) depth

9.4in (23.9cm) height without Tablet PC mounted
18in (45.7cm) height with Tablet PC mounted

Weight Approximately 40lb (18.1kg)

Power Requirements 0.5A @ 100–240V AC, 50/60Hz (universal)

Warranty 1-year parts and labor warranty included

Regulatory Compliance For Research Use Only. Not for use in Diagnostic Procedures.

CE Compliance Pollution degree 2

Installation category II
Indoor use only
Mains supply voltage fluctuations are not to exceed ±10 percent of
the nominal supply voltage
This instrument complies with the requirements of the EU Directives
for electromagnetic compatibility (EMC Directive) and for electrical
safety (the LVD). Compliance with the relevant Directives was
demonstrated by third party testing to the appropriate EN and IEC
standards. The product bears the CE mark on its label as evidence of
compliance and an EU Declaration of Conformity for the product is
available from Promega on request.

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1.B. Specifications (continued)

Luminescence Module Specifications

Detector Top-reading, head-on photon-counting photomultiplier tube (PMT)

Wavelength Range 350–700nm

Detection Limit 1.5 × 10

–21

moles of luciferase

Dynamic Range >9 logs

Cross Talk Less than 3 × 10

–5

(white, 96-well plate, Corning Cat.# 3912)

Injector System Specifications

Number of Injectors (optional) Two injectors

Dispense Volume Range 5–200µl in 1µl increments

Injection Speed 20–500µl per second

Waste Collection Tray volume Approximately 50ml

Void Volume 500µl

Computer Requirements

Operating System Windows

®

8 or 8.1, 64 bit, with USB connection to

instrument

RAM 2GB (4GB preferred)

Disk Space 64GB

Other Requirements Microsoft.NET Framework 4.0 (full version),

Microsoft

Excel 2013 Professional

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1.B. Specifications (continued)

Additional Computer Recommendations if Using Your Own PC:

®

• Disable automatic Windows

updates and virus scans

• Disable automatic power off, sleep or hibernation settings

Disable "Fast Startup" in Windows

®

8. Set the power button to Shut Down, not Sleep.

Disable any other automatically or manually started software that would consume PC resources during
operation.

• Ensure there are no domain policies that would overwrite any of the above settings.

• Ensure that Windows

®

will not turn off USB ports to save power.

*Note: The Tablet PC that is provided with GloMax

®

Navigator has already been configured for the

settings listed above.

1.C Product Components

PRODUCT SIZE CAT.#

GloMax® Navigator System 1 each GM2000

For Research Use. Not for use in Diagnostic Procedures. Includes:

• 1 each GloMax

• 1 each Tablet PC containing GloMax

®

Navigator Instrument

®

Navigator Software

• 1 each Tablet PC AC Power Adapter

• 1 each Tablet Mount Attachment

• 1 each AC Power Adapter with detachable power cord

• 1 each USB Cable

• 1 each 2.5mm Wrench

PRODUCT SIZE CAT.#

GloMax® Navigator System with Dual Injectors 1 each GM2010

For Research Use. Not for use in Diagnostic Procedures. Includes:

• 1 each GloMax

• 1 each Tablet PC containing GloMax

®

Navigator Instrument

®

Navigator Software

• 1 each Tablet PC AC Power Adapter

• 1 each Tablet Mount Attachment

• 1 each AC Power Adapter with detachable power cord

• 1 each USB Cable

• 1 each 2.5mm Wrench

• 1 each Dual Injectors (installed)

• 1 each Reagent Bottle Holder

• 1 each Waste Collection Tray

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1.C Product Components (continued)

Available Separately

PRODUCT SIZE CAT.#

Dual Injectors and Pump Staon Upgrade for GloMax® Navigator 1 each SA1304

GloMax® Systems Luminometer Light Plate 1 each E5141

1.D. Precautions and Special Instructions

Safety Symbols and Special Markings

Danger. Hazardous voltage. Risk
of electrical shock.

Warning. Risk of personal injury
to the operator or a safety hazard
to the equipment or surrounding
area.

It is important to understand and
follow all laws regarding the safe
and proper disposal of electrical
instrumentation. Please contact
your local Promega Representative
for disposal of the instrument and
power supply. Please follow your
institutional requirements for
disposal of the accessories.

Danger. Tension dangereuse.
Risque de choc électrique.

Avertissement. Risque de
préjudice corporel pour
l’opérateur ou d’accident avec
l’instrument ou l’entourage.

Avertissement. Il est important
de comprendre et de respecter
toutes les lois relatives à la
destruction sure et correcte des
appareils électriques. Veuillez
contacter votre représentant
Promega local concernant la
destruction de l’appareil.Veuillez
respecter les exigences de votre
établissement concernant la
destruction des accessoires.

Clean up spills. Nettoyer les déversements.

13631TA

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Special Instructions

• Wipe up spills immediately.

• The GloMax

®

Navigator Instrument contains sensitive optical components and precision-aligned

mechanical assemblies. Handle with care.

• Use caution around solvents because they may damage the plastic case of the GloMax

®

Navigator

Instrument.

• Do not submerge the GloMax

• Do not expose the GloMax

®

Navigator Instrument in water.

®

Navigator Instrument to temperatures outside the specified range, as

damage may occur to the unit that will not be covered under warranty.

• Changes or modifications to this unit not expressly approved by Promega could void the user’s authority
to operate the equipment.

• Do not use this device in proximity to sources of strong electromagnetic radiation (e.g., microwave oven)
because they may interfere with the proper operation.

• Do not use this instrument for anything other than its intended use.

• Always disconnect the power before cleaning or performing routine maintenance.

• Do not disassemble the instrument further than specified in this operating manual for routine
maintenance and use.

• If the equipment is used in a manner other than that specified by Promega, the protection provided by
the equipment may be impaired.

• Do not overfill multiwell plate wells because this may lead to spills and/or damage.

• If injectors are installed, check the tubing periodically and replace as needed to avoid leaks.

• Plug instrument and Tablet PC into the same wall socket or outlet strip to assure common grounding.

• Do not leave plate in the instrument after protocol is completed.

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1.D. Precautions and Special Instructions (continued)

Precautions

Important Safety Instructions—Please save these instructions.

Power OFF the GloMax® Navigator
before accessing the interior,
installing or cleaning injectors.

Close the instrument door when the

®

GloMax

Navigator is not in use.

The GloMax® Navigator is intended
for indoor use only.

Always disconnect the power
before cleaning or performing
routine maintenance.

Always disconnect the AC Adaptor
from the power outlet when not in
use.

Mise hors tension de GloMax

®

Navigator avant d’accéder à
l’intérieur, l’installation ou le
nettoyage des injecteurs.

Fermez la porte de l’appareil
lorsque le GloMax® Navigator n’est
pas en cours d’utilisation.

Le GloMax® Navigator est destiné à
une utilisation en intérieur.

Déconnectez toujours
l’alimentation avant de nettoyer ou
d’effectuer l’entretien de routine.

Toujours débrancher l’adaptateur
secteur de la prise de courant
lorsqu’il n’est pas utilisé.

1.E. Environmental Requirements

Store and use the GloMax

®

Navigator under the following conditions:

Shipping and Storage Conditions 4–50°C, under noncondensing conditions, up to 75%

humidity

Operating Conditions 5–30°C, up to 75% humidity

Operating Altitude Conditions less than 2,000 meters above sea level

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1.F. Inspection and Unpacking

13595T

Upon receiving the GloMax

®

Navigator System, inspect it carefully and make certain all accessories are included.
Refer to the checklist shipped with the instrument for order-specific items. Save all packaging materials, if
possible, in case the instrument needs to be returned for service. If any item is damaged, contact Promega
Technical Services (E-mail: [e-mail]). Standard components are shown in Figure 2, Panel A,
below.

A B

C

A

Figure 2. GloMax® Navigator System Accessory Components. Panel A. Components shown include:
Tablet PC, Tablet PC Stylus, Tablet Fitting for mounting the Tablet PC, Tablet PC AC Power Adapter, Instrument
Power Cord, Instrument AC Power Adapter, USB Cable and 2.5mm Wrench. Panel B. Reagent Bottle Holder (for
GM2010 model only). Panel C. Waste Collection Tray (for GM2010 model only).

1. Remove the instrument and accessory box from the shipping container.

A

Figure 3. Contents of the GloMax® Navigator System accessory box.

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1.F. Inspection and Unpacking (continued)

2. Place the GloMax

®

Navigator Instrument on a flat, level surface.

3. Ensure that the instrument is placed in a location that will allow you to meet the power requirement
specifications (100–240V AC, 50/60Hz; Section 1.B).

4. Manually open the door of the instrument, and remove the interior foam packaging that prevents the optical
head from moving during shipment.

2. Setting Up the GloMax

2.A. Setting Up the GloMax

®

Navigator System

®

Navigator Instrument and Tablet PC

1. Unpack the Tablet PC from its shipping box.

2. Place the Tablet PC in the Tablet Holder on the top of the GloMax

®

Navigator Instrument in the orientation

shown in Figure 4.

13516TA

Fi

gure 4. Instrument front and Tablet PC holder setup.

3. Secure the Tablet PC in the Tablet Holder by using the 2.5mm Wrench to screw the Tablet Mount Attachment
Piece into place.

4. Ensure that the instrument On/Off switch is in the OFF position (Figure 5).

5. Using the provided USB cable, connect the Tablet PC to the USB port on the back of the instrument (Figure 5).

6. Connect the Tablet PC to its power supply, and then connect the Tablet PC Power Cable to a power outlet.

7. Connect the Instrument AC Power Adapter to the back of the instrument and to a power outlet.

Note: We recommend connecting the instrument to an uninterrupted power supply (~500VA).

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Instrument lid

Outlet tubing

Tubing guide

Injector syringe

Inlet tubing

Reagent bottle
holder location

Sample tray
latch

F

igure 5. Interior view of the GloMax® Navigator Instrument

ABCD

LED status
light

Optical head

Injector tip holder

Microplate sample
tray and cover

13542TB

13517TB

Figure 6. The back of the GloMax® Navigator Instrument. A. On/Off switch. B. Power connection.

C. USB port to connect to Tablet PC. D. Two USB ports.

2.B. Switching On the GloMax

®

Navigator Instrument

1. Switch the instrument ON.

2. An LED light on the front right corner of the GloMax

®

Navigator indicates when the instrument is ON.

3. Power up the Tablet PC by pressing and holding the power button on the perimeter of the Tablet PC.

Note: When powering ON, turn the instrument ON first and then the Tablet. A connectivity message may

appear in the software if the Tablet is powered ON before the instrument. If that occurs power OFF the
Tablet and then power ON the Tablet.

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2.B. Switching On the GloMax® Navigator Instrument (continued)

13565T

4. The Tablet PC will begin its start-up protocol. After the operating software starts, tap on the GloMax
Navigator Software Icon to launch the GloMax

5. The GloMax

®

Navigator System is now ready to use.

®

Navigator software.

®

Exit Back

F

igure 7. GloMax® Navigator software Home Screen.

2.C. Shutting Down the GloMax

®

Navigator Instrument

Tools Eject Help

A

1. Switch the instrument OFF using the On/Off switch on the back of the instrument.

2. Shut down the GloMax

3. Shut down the Tablet PC using normal Windows

®

Navigator Software by tapping on the “x” in the upper right corner (Figure 7).

®

8 shutdown procedures: Swipe in from the right edge of

the screen, select Settings, then select Power: Shut Down.

Note: When not in use for a prolonged period of time, the Tablet PC should be powered OFF.

!

If the Tablet PC is switched ON but is not connected to its power supply, it will go to Sleep mode after

15 minutes of inactivity. This does not happen if the Tablet PC is connected to its power supply.

2.D. Operating the GloMax

®

Navigator System

1. Open the door of the instrument and gently press the Microplate Sample Tray latch to release the tray cover
(Figure 5). If a lid is present, remove from plate. Insert an opaque 96-well plate such that well A1 of the
plate corresponds to the label “A1” on the plate tray (Figure 8).

2. Close the sample tray cover and the instrument door.

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Figure 8. Inserting plate into sample tray.

13596T

3. Select one of the Quick Reads protocols from the Protocols Window or from the yellow Quick Read
option on the Home screen.

4. Select the Start button at the bottom right corner of the screen.

5. Enter the name of the result file by touching inside the text box and entering a name (Figure 9).

A

Figure 9. Save Results Window.

6. Select Start to begin the protocol.

7. The protocol will begin acquiring data and will display the results in the plate format in real time on the
screen.

8. When the read is finished, an Export button will be active on the bottom left corner of the screen, if you wish
to export the results. Results are exported to the location specified in Settings -->Administrator-->Export
Folder (Section 3.E, Export Folder). If you do not export the results, but want to export them at a later time,
you can find the results by touching the Results button on the home screen.

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3. GloMax® Navigator User Interface Screens

13565TA

Exit Back

Tools Eject Help

3.A. Tablet PC Home Screen

The Home Screen on the Tablet PC is the main launching pad for interaction with the functionalities built into
the GloMax

®

Navigator Graphical User Interface Software. The Home Screen (Figure 10) contains 4 buttons:

Luminescence. This Quick Read button allows you to immediately perform a luminescence read, selecting the
plate map and Integration time.

Protocols. Pressing the Protocols button will provide you a list of all available protocols. These include preloaded
protocols as well as user-defined protocols. The user can create user-defined protocols from this screen as well.
Smart Protocols provide analysis directly after the measurement is completed.

Results. Pressing the Results button will provide the user a list of all results. The user can view results by today,
this week, this month, the last 3 months, the last 6 months, anytime this year, or all of the results.

Settings. Pressing the Settings button provides the user with instrument information and setup options. For
example, one can name the instrument, specify where files are exported on the PC or Network drive, export log
files, and access additional Tools.

Figure 10. GloMax® Navigator Home screen.

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3.A. Tablet PC Home Screen (continued)

13597TA

Select Integration
time.

Start
measurement.

Define the Plate Map. Green = selected, Gray = unselected

The Instrument name appears in the title bar of the user interface. The following navigation buttons are displayed
across the top of the user interface window:

Exit. Tapping this button allows you to exit the GloMax
Windows

®

Operating System.

®

Navigator User Interface software and returns to the

Back. Tapping this button returns you to the previous window.

Tools. Tapping this button expands a list of available tools such as accessing the injectors to Prime, Reverse Purge

and/or Flush and accessing the instrument information (About).

Eject. Tapping this button will move the Plate Tray to the Home position to add or remove a plate from the
instrument.

Help. Tapping the help button activates the context-sensitive help for the current page of the GloMax

®

Navigator

User Interface.

3.B. Quick Read Screen

Quick Reads are intended to allow users to perform a basic single-step measurement very easily. Simply define the
plate map and the integration time for luminescence. Open the door, insert your plate, and press the Start button
in the lower right corner to begin the plate read.

Figure 11. GloMax® Navigator Luminescence Quick Read Screen. On the plate map, green wells are
selected.

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3.C. Protocol Screen

13600T

Select favorites.

13599T

Touch Protocols from the Home Screen to access the list of protocols currently present in the software or create
a new protocol. The user can view user-defined protocols by selecting User, view Promega preloaded protocols by
selecting Preset, run a Promega Smart Protocol (see below for more details) or see all protocols by selecting All.
Favorite protocols can be marked by tapping the Star adjacent to the protocol name. After marking a protocol as a
“favorite”, those protocols will automatically be listed at the top of the Protocol Screen to make locating them
easier. There is no limit to the number of protocols that may be selected as favorites.

View protocols
of interest.

Create new

A

protocol.

Figure 12. GloMax® Navigator Protocol Screen.

Only user-defined protocols may be deleted, by tapping the red X at the far right of the screen. After selecting
Delete, the user will be asked to confirm the request to delete the user-defined protocol.

A

Figure

13. Delete Protocol message from the GloMax® Navigator software.

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Smart Protocols

Protocol name and estimated duration.

Smart Protocols are designed to provide the user with a guided setup of specific assays. Smart Protocols titled
“Demo” should not be run for any other purpose than a demonstration of an assay kit. Following the data
collection, the results are automatically analyzed for the user.

1. Tap the Smart Protocol button, and select the protocol of interest.

2. Follow the on-screen instructions. Insert the plate into the instrument.

3. Tap OK when ready to begin the protocol. The protocol will begin when OK is selected, so ensure the plate
has been loaded into the instrument.

4. When the read is complete, the user will be prompted to export the results. Upon selecting export, the raw
data will be exported along with an analysis of the data for the user.

Viewing Existing Protocols

1. To view details of an existing protocol, from the Protocols Screen select the protocol of interest.

2. The protocol details will appear. If any changes are made to the protocol the user has the ability to Save or
Save As and provide a new name.

Expand protocol steps
for more detail.

13598TA

Plate map for
global settings.

Save as, Save and
Start protocol.

Figure 14. GloMax® Navigator Protocol Screen.

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3.C. Protocol Screen (continued)

11630T

13601TA

Drag and drop
protocol steps
into white area.

Creating a New Protocol

Basic Protocol Steps

1. To create a new protocol, from the Protocols Screen tap the New Protocol button at the bottom of the
screen (Figure 12).

2. You will be prompted to name the protocol. You also may provide a description, if desired.

A

Figure 15. New Protocol name prompt from the GloMax® Navigator software.

3. Build your protocol by dragging and dropping protocol functions from the left panel onto the Protocol
Window.

F

igure 16. GloMax® Navigator New Protocol Screen showing drag and drop feature to create custom

protocols.

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13602T

A

11633T

Figure

17. GloMax® Navigator New Protocol screen showing a step added to the Protocol Window.

4. Select filters, integration times and plate map (see below) as you build the protocol.

5. Rearrange steps by dragging and dropping as desired.

6. Delete steps by selecting the black X in the top right corner of the step.

7. Save the protocol by selecting the Save As button on the bottom right corner of the screen. A user message
will appear indicating that the protocol was saved successfully.

A

F

igure 18. GloMax® Navigator New Protocol Successfully Saved user message.

Plate Maps

There are two ways to select a plate map for the measurement. The default is to apply the plate map to all steps
within a protocol. In the upper right corner of the Protocol Window, the user can lock or unlock the plate map.
When locked (which is the default), the plate map is applied to each step of the protocol.

When the plate map is applied to each step of the protocol, only the plate map in the upper right corner may be
modified. The plate maps within each step of the protocol are then shaded and cannot be modified.

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3.C. Protocol Screen (continued)

13604TA

Plate Map is
locked.

Select Plate Map.

Select Plate Map

for each protocol

step.

13605TA

Plate Map is
unlocked and
cannot be
applied.

Select Plate Map

for each

protocol step.

Figure 19. When the plate map is locked, the map is applied to each step of the protocol.

When the plate map is unlocked, each step of the protocol can have a different plate map. The plate map in the
upper right corner in this case is shaded and cannot be modified, whereas the plate maps within each step of the
protocol are now active and the user can modify each step as desired.

Figure

20. When the plate map is unlocked, a different plate map can be created for each step.

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Whenever a protocol is locked, the plate map that is indicated in the upper right corner will apply to each protocol
step. Notice in Figure 20, step 1 of the protocol has columns 1–6 selected, and step 2 has columns 7–12 selected.
When locking the protocol, each step will revert to whatever new plate map is selected.

Luminescence Integration Times

Integration times for luminescence reads may be selected from 0.1 to 10 seconds.

TM470 · 4/16 www.promega.com

Protocol Steps

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A variety of protocol steps can be used to customize your protocol of interest. These functions are added by
dragging the button function in the left column of the New Protocol screen into the middle of the Protocol

Window.

The functions can be expanded and contracted by selecting the black triangle at the top left corner of each
function. They may be removed by selecting the black X at the top right corner of each function.

A

Figure 21. GloMax® Navigator New Protocol Functions.

Wait Function

Use the Wait function to pause or incubate before taking a measurement. After selecting the duration time
window, a keypad will appear in which you can enter the time in hh:mm:ss format. Enter between 1 second and 72
hours in increments of 1 second.

F

igure 22. Wait function screens.

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3.C. Protocol Screen (continued)

13608TA

Inject Function

Dual Injectors are an optional accessory for the GloMax

®

Navigator Instrument. On instruments equipped with
dual injectors, you can choose which injector to use (Injector 1, Injector 2 or both), the desired injection volume
(5µl–200µl) and the injection speed (20µl/sec to 500µl/sec). Select the wells that will be injected by touching the
plate map and selecting the desired wells.

Warning: Make sure sufficient space is available in the well to accept the injected volume. Failure to do so may

!

cause an overflow of reagent and/or sample into adjacent wells or into the interior of the instrument. See Cleaning
and Maintenance (Section 5) if reagent is spilled inside the instrument.

The injectors may also be accessed from the Tool icon in the menu bar.

See Setting Up and Operating the Injector System (Section 4) for more details.

Figure 23. Inject Function.

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Prompt

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13609T

Use a message prompt to provide the user with instructions. Tap Enter Message in the prompt, and a keyboard
will appear for the user. Type a user message, then tap OK to enter it into the protocol.

A

Figure

24. Prompt Function.

When the Eject Plate Prompt button is checked, the Prompt screen shown in Figure 25 appears to the user during
protocol, with the option to open the door.

If Eject Plate is selected, the instrument will home and the user can then open the door and remove the plate. After
returning the plate into the Plate Tray and closing the door, the user may then select Continue. When Continue is
selected, the protocol continues to the next step. These prompts and commands are useful if performing a
multi-step protocol where the user must add reagent to the samples between measurements prior to continuing the
protocol.

A

Figure 25. Prompt window with the Eject Plate option.

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3.C. Protocol Screen (continued)

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13612T

Loop

Loops are used to perform a set of steps on a per-plate basis over a fixed time interval, then repeating the set of
steps a desired number of times.

Note: The interval (min) field defines the minimal interval of time for one loop iteration. If all of the operations
within the loop are completed before the specified interval time has passed, the software will wait for the specified
interval. If all of the operations take longer than the specified interval time, the software will continue to the next
iteration without waiting.

Note: Loops can be used with luminescence detection, protocol functions and plate types. A maximum of 100
loops can be performed within a single protocol.

1. Add the Loop step to the protocol by dragging and dropping the Loop icon into the protocol window.

A

F

igure 26. Loop function.

2. Select the desired number of iterations.

3. If the same plate map will be used for each step of the protocol, lock the plate map in the upper right corner

to select the wells.

If a different plate map is to be used for various steps, unlock the plate map in the upper right corner, then

select the plate map icon within each step of the loop to select the desired wells.

4. Begin building the desired workflow by dragging and dropping the desired steps inside the Loop step. They

should be contained in the Loop step.

Note: If adding an injector step inside a loop function, ensure that there is sufficient space in the well to

!

accommodate multiple additions of reagent.

5. Rearrange the steps by touching and dragging the appropriate steps.

A

Figure 27. Loop function with with a Luminescence step within the Loop container.

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Kinetics Function

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The Kinetics tool is used to perform a set of steps on a per-well basis, then repeating the set of steps a desired
number of times (i.e., inject and read one well multiple times before proceeding to the next well).

Note: Kinetics can be used with all protocol functions except for user prompt functions. Loops may not be placed
within a kinetic step, but kinetic steps may be placed within Loops. A maximum of 250 kinetic reads in 1-unit
increments can be performed within a single protocol. For example, 10 readings with a 1-second interval means
that 1 reading is taken every 1 second for 10 seconds.

1. Add the Kinetics step to the protocol by dragging and dropping the Kinetics icon onto the Protocol
Window.

A

Figure 28. Kinetics function.

2. All well-associated operations within the kinetic block must have the same well selection. The plate maps of
these operations are not individually editable.

3. Begin building the desired workflow by dragging and dropping the desired steps into the kinetic step. They
should be contained within the kinetic step.

A

F

igure 29. Kinetic Function with a Luminescence step within the Kinetic container.

4. Select the number of readings and interval (seconds) between each reading.

Note: Typically the protocol steps are completed before the completion of the Interval Time, and therefore

the instrument will pause until the completion of the Interval Time duration. However, if the protocol steps
take longer to complete than the Interval Time, the protocol will complete all steps and then immediately
continue without pausing for the Interval Time duration. Otherwise, once all steps are completed the protocol
will pause until the Interval Time duration has been reached.

5. Rearrange the steps by touching and dragging the step.

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3.D. Results Screen

13615T

View results within a group by sorting on date/time, name or user.

When running a protocol, the results will display in real-time as the protocol proceeds. Results are automatically
saved within the GloMax

®

Navigator Software. However, they can also be exported to a variety of locations,

including the local Tablet PC, to a USB flash drive, a network drive, or to a cloud location.

1. To view results from previous runs, from the Home screen, tap the Results button.

a. In the Left Navigation column the user can view results by today, this week, this month, the last three

months, the last 6 months, anytime this year or all of the results.

b. Select the result file of interest to view the results.

Select to remove

or export file.

Export or remove

1 or more results.

A

Figure 30. GloMax® Navigator Results Screen.

2. To view results in real-time as a protocol is running, the plate map will automatically appear on the screen,
and the data will appear as they are collected. If more than one measurement is taken, each result may be
displayed by selecting the step in the left navigation column.

Results are shown in a heat map format to indicate wells with higher or lower signals in comparison to

the entire plate (Figure 32). When the data are collected, the software automatically scales the color
selection, assigning the highest values red and the lowest values blue, along a gradient.

7.56e+07 1.02e+07 1.03e+06 9.82e+04 1.31e+04 3.22e+03 298

72

11834MA

Figure 31. Example GloMax® Navigator Results heat map colors.

Within the plate map, each individual sample may be viewed by tapping the well of interest.

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13619T

Select
measurement 1
or measurement 2
to view results.

Turn the heat
map on or off.

A

Figure 32. GloMax® Navigator Results screen with heat map display.

Kinetic results display a graphical representation of the data during the course of the kinetics read

(Figure 33). Data are automatically scaled based on the results within each well. Zoom in to view the
axis for each well as desired. Users can use touch-screen gestures to zoom in or out, or to pan the well
map.

Figure 33. Example of result screen for kinetic data.

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3.D. Results Screen (continued)

Figure 34. Example of individual well result for kinetic data.

3. Once a read is complete, the Results screen will appear (see Figures 32 and 33).

a. To Export data, tap the Export button (bottom left corner of screen in Figures 32 and 33).

b. The software will then open the Export folder that was selected in the Administrator settings (see

Section 3.E, Figure 45). Data may be exported to a number of locations, including a USB flash drive,
the Tablet PC C drive or a cloud-based storage.

c. Two files are automatically exported: a comma-separated value file, and a Microsoft Excel worksheet

file. If creating a file for use with a LIMS, use the CSV file and import according to your LIMS software
guidelines.

Figure 35. File Export. Two data files are exported (CSV and Excel® files).

d. To open, double-tap the file.

e. Results for the Excel

®

file open directly within Microsoft Excel® or Excel® viewer. Multiple tabs are
located in the spreadsheet to view the data in plate map format or linear format by well. All settings
used in the protocol are also displayed in the workbook. If multiple readings were taken, they each are
displayed as you scroll down the page.

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F

11656TB

13620T

igure 36. File Export to Microsoft Excel® Workbook.

Note: Colors in the Excel

®

workbook will not reflect those within the software heat map, and therefore you
will need to place the data into cells of similar shades if you wish to maintain the heat map color scheme in
the Excel

F

igure 37. File Export to Microsoft Excel® Workbook. Data with time intervals. Each time value is based on

®

workbook.

A

when the first read was started (T = 0 seconds).

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3.E. Settings Screen

13621T

13622T

A

Figure 38. The GloMax® Navigator Settings Screen.

Instrument Info

Tapping the Instrument Info button reveals a list that includes the software and firmware versions, the instrument
name and maintenance and calibration values.

A

Figure 39. Settings  Instrument Info screen located on the Settings menu. Figure 39 shows example
information only. Your instrument information may vary.

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Self Test

13623T

Each time the instrument is powered “ON”, it automatically performs a self test to check the motion of all axes.
This button allows users to manually initiate a self test.

Tools

Tools may be accessed from the Tools icon in the menu bar (shown below) or from the Settings  Tools window.

A

Figure 40. Tools icon.

Injectors

Dual Injectors are an optional accessory for the GloMax

®

Navigator Instrument.

Injector System Specifications

Number of Injectors Two Injectors

Dispense Volume Range 5–200µl in 1µl increments

Injection Speed 20–500µl/second*

Waste Collection Tray Volume Approximately 50ml

Void Volume 500µl

®

*For standard Luciferase Assay or Dual-Luciferase

Assays, we recommend an injector speed of 200µl per

second.

Warning: Make sure sufficient volume is available in the well to accept the injected volume, especially when

!

injectors are used within a loop function. Failure to do so may cause an overflow of reagent and/or sample into
adjacent wells or into the interior of the instrument.

The Injectors may also be accessed from the Tools icon in the menu bar.

See Setting Up and Operating the Injector System (Section 4 for more details).

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3.E. Settings Screen (continued)

Recovery

Recovery provides the user the ability to manually initialize the instrument axes in case of a severe plate jam. This
option is available only from the Settings --> Tools window. In the event of a plate jam, the instrument will
automatically initialize instrument axes itself when either the Door button is selected, a Protocol is started, or if
the instrument power is turned Off/On.

Initialize all axes by selecting the Start button in this screen.

13633TA

Figure 41. Recovery options.

Export Logs

The software records all actions performed by the instrument. If users experience error messages, export the log
and send it to Promega Technical Services ([e-mail]) for troubleshooting. The log file is useful
for troubleshooting events.

Upon tapping the Export Logs button, the logs will export to the Tablet PC at the following location:

C:\Exports\Logs

Figure 42. Export Logs screen.

Figure 43. Export Logs location.

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Administrator

The Administrator button allows you to customize the Instrument name, specify folder locations to which results
should be exported, and import protocols for the instrument.

13624TA

F

igure 44. Settings  Administrator screen.

Export Folder

Select the folder location to which the data should be exported. Once this is configured, the data will be exported
to this location. This includes exporting to a USB flash drive or network location.

Tap the Drive\ button at the top to view the available drive locations. Then tap the specific drive to navigate to the
folder location of interest.

Select Save when finished.

Figure 45. Settings  Administrator screen  Export Folder on the Settings menu.

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3.E Settings Screen (continued)

12724T

Instrument Name

Enter the desired name of the instrument. This feature is typically used if a laboratory has more than one GloMax
Navigator Instrument. The instrument name will be included in the results files.

Note: Changing the instrument name will force a reboot of the Tablet PC.

!

A

F

igure 46. Settings  Administrator screen  Instrument Name.

Import Protocols

When new protocols are available, the administrator may import new protocols with instructions directly from the
Promega web site: www.promega.com/resources/tools/detection-instruments-software/promega-branded-

instruments/glomax-navigator/

Download the protocol file from the web site and save to a USB flash drive, network location, local Tablet PC, etc.

From the GloMax

®

Navigator Software Import Protocols window (SettingsAdministratorImport Protocols),

navigate to the folder location and select the file.

Select on the Drive\ button at the top to view the available drive locations. Then select the specific drive to
navigate to the location of the protocol package file. Protocol package files will appear as blue rectangles in the
Select File Window. Touch the package file that you wish to import.

®

Select OK when finished.

Figure 47. Settings  Administrator screen  Import Protocols.

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Audit Records

13625T

Administrators may view a chronological sequence of actions performed with the GloMax

®

Navigator System by
selecting the Audit Records button within the Administration screen. Records can be viewed on the left side of the
screen by Today, This Week, the past 3 Months, the past 6 Months, This Year, or by All. Detailed audit records are
automatically generated and stored with the GloMax

®

Navigator software that cannot be deleted or edited in order

to provide technical elements of a part 11 compliant system when used with the appropriate laboratory workflow.

Records may be exported by selecting the Export All button on the lower left corner of the screen.

Figure 48. Audit Records screen.

Help

The context-dependent Help Screen can be accessed from any of the menu screens.

A

F

igure 49. Help Screen.

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4. Operating the Injector System (optional)

Injectors can be used with basic protocols, as well as with all Kinetic and Loop protocols.

Warning: Make sure sufficient volume is available in the well to accept the injected volume. This is especially

!

important for Kinetic and Loop protocols where multiple injection steps may occur. Failure to do so may cause an
overflow of reagent and/or sample into adjacent wells or into the interior of the instrument.

You can define the injection volume and speed, delay time and integration time for each of the two injectors using
the Protocol builder from the Home screen. This information is saved with each protocol.

The viscosity of solutions/reagents can affect the performance of the injectors.

If a protocol requires injectors, the injectors must first be primed with at least 500μl of the reagent to be injected.
Priming wets the fluid path and removes air bubbles to ensure delivery of an accurate injection volume for the
assay.

Priming is also required if an injection protocol has been interrupted for any reason (e.g., power failure).

You may need to optimize injection speed, depending on the solution/reagent you are working with. We recommend
using the default settings of 200µl per second as a starting point. From the Tools icon in the menu bar, or accessed
from Settings  Tools (Figure 38), follow the guided instructions to Prime, Reverse Purge and Flush the injectors.

Figure 50. Injector functions (prime, reverse purge and flush).

After finishing an assay, unused reagent in the fluid path can be retrieved. Select the option to Reverse Purge by
tapping the “Reverse Purge” button on the Injectors screen. To prevent clogging of the fluid lines, flush after each
use.

Note: The reagent bottle holder can accommodate four sizes of containers: a round flat-bottom bottle, a 50ml
conical tube, a 15ml conical tube and a 15ml test tube.

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4.A. Priming Injectors

13626TA

1. Place the reagent container (tube or bottle) into the reagent holder.

2. Cover the bottle opening with Parafilm

3. Insert the injector tube through the Parafilm

®

to prevent reagent evaporation.

®

and into the reagent bottle.

4. Ensure the Waste Collection Tray is in position and empty.

5. Tap the Start button adjacent to the Prime Injectors information in Figure 50.

Figure 51. Injector prime function.

6. Indicate the injector to prime. Set the volume.

Note: The minimum volume needed to prime is 500µl.

7. Tap the Start Prime button (Figure 51).

8. After priming is complete, empty the Waste Collection Tray.

Once the injectors are primed, they are ready for use according to the programmed protocol.

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4.B. Reverse Purge (Retrieving Unused Reagents from Injectors, optional)

13627TA

1. Tap the Start button adjacent to the Reverse Purge option on the Injectors screen (Figure 50).

Figure 52. Reverse Purge function.

2. Ensure the Waste Collection Tray is in position and empty.

3. Tap the Start Reverse Purge button (Figure 52).

4. Any reagents left in the fluid lines will be returned to the reagent container.

4.C. Flushing the Injectors after Use

1. Remove the sample plate from the instrument.

2. Tap the Start button adjacent to the Flush option on the Injectors screen (Figure 50).

13628TA

Figure 53. Injector Flush function.

3. Ensure the Waste Collection Tray is in position and empty.

4. Tap the Start Flush button (Figure 53).

The recommended flush protocol uses a sequence of 1 cycle (1ml) of deionized water, 1 cycle (1ml) of 70%

ethanol, 1ml of deionized water and then 1 cycle (1ml) of air to flush the fluid.

Note: Empty Waste Collection Tray when priming and flushing are complete. See Section 5.F for injector

cleaning instructions.

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5. Cleaning and Maintenance

5.A. General Instrument Care

If a well is overfilled, immediately clean up any spills.

To gain access to the inside of the instrument, manually open the door.

5.B. General Cleaning

Turn off the GloMax

®

Navigator System and disconnect the AC Power Adapter whenever the interior of the

instrument is open for cleaning and maintenance.

Residue accumulated from various reagents may inhibit proper movement of the Aperture cross talk mask.
Immediately clean up any spills. We recommend thoroughly cleaning the interior of the instrument every 30 days
as follows:

Do not allow excess solution to run off onto other electrical components, because this may cause damage to the
instrument and its electronics. Do not use solvents or abrasive cleaners.

Use a cloth dampened with deionized water to periodically wipe clean the exterior of the GloMax

®

Navigator

Instrument. Do not use solvents or abrasive cleaners.

Warning: If a fluid spill on the detector is suspected, immediately contact Promega Technical Services for

!

cleaning instructions.

5.C. Tablet PC Care

Do not use a sharp object, such as a pen, pencil, or marker to make contact with the touch screen. Sharp objects
may damage or leave a permanent mark on the LCD screen. Do not spill liquid on the screen.

To clean the Tablet PC, turn off the power. The touchscreen has been coated to make it easier to clean. You don’t
need to rub hard to remove fingerprints or oily spots. To avoid scratches, use a soft, lint-free cloth to gently wipe
the screen. You can dampen the cloth with water or an eyeglass cleaner, but don’t apply liquids directly to the
Tablet PC. Don’t use window cleaner or other chemical cleaners on the Tablet PC.

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5.D. Cleaning the Optical Head

1. Turn OFF the instrument. Disconnect the AC Power Adaptor. Do not attempt to clean the Optical Head
while the instrument is ON.

2. Push the Microplate Sample Tray to the back of the instrument and push the Optical Head from the Home
position toward the center of the instrument (Figure 54).

Optical head

Microplate sample
tray and cover

13542TC

Fi

gure 54. Interior of the GloMax® Navigator instrument.

3. Loosen the hand screws and remove the Injector Tip Holder from the Optical Head. Facing the front of the
instrument, push upward and then leftward (as viewed from the bottom of the Tip Holder to release the
Tip Holder from the Optical Head (Figure 55). Completely remove the Injector Tip Holder to take out the
Aperture.

F

igure 55. Remove the Injector Tip Holder.

4. Place your hand underneath the Optical Head. Grasp the Aperture and pull it toward you (Figure 56).

Note: Do not touch the bottom surface of the Optical Head.

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Figure 56. Remove the Aperture.

5. Soak the Injector Tip Holder and the Aperture in a 70% ethanol solution for 30 minutes to dissolve reagent
residue. Rinse with deionized water.

6. Allow the Injector Tip Holder and Aperture to air dry completely before proceeding. Alternatively, blot dry
with a lint-free, Kimwipes

®

tissue.

7. Return the Aperture to the Optical Head. Align the Aperture with the Optical Head and gently guide the
Aperture onto the Optical Head (Figure 57).

F

igure 57. Replace the Aperture.

8. Return the Injector Tip Holder to the Optical Head and tighten the hand screws. Position the holder just
above the front pins on the Optical Head. Push the holder toward the right to lock the holder securely to the
right pin of the Optical Head. Then push down and lock the holder on the left pin (Figure 58).

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5.D. Cleaning the Optical Head (continued)

Figure 58. Replace the Injector Tip Holder.

9. Return the Optical Head to the Home position by pushing the Optical Head from the center of the instrument
all the way to the right side of the instrument (Figure 54).

10. Pull the Microplate Sample Tray forward to the Home position.

11. Reconnect the AC Power Adaptor to the instrument. Turn ON the instrument.

5.E. Cleaning the Instrument Interior

Accumulated residue from various luminescent substrates may inhibit proper movement of the Optical Head.
Failure to properly maintain a clean interior may cause a mechanical error and the irretrievable loss of data. We
recommend thoroughly cleaning the interior every 30 days.

1. Turn OFF the instrument

2. Use a Kimwipes

®

tissue dampened with 70% ethanol to wipe the Microplate Sample Tray cover. Repeat.

3. Push the Microplate Sample Tray to the back of the instrument, exposing two steel guide rails upon which
the sample tray glides (Figure 54). Use a Kimwipes

®

tissue dampened with 70% ethanol to clean the guide

rails.

4. Push the Optical Head to the center of the instrument, exposing the black metal shelf on the right side of the
instrument (Figure 55).

5. Clean the black shelf with a fresh Kimwipes

®

tissue dampened with 70% ethanol. Be sure to clean the home

position for the optical head.

6. Close the instrument door and turn the instrument ON. The instrument will then return to the Home
position and is ready to use.

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5.F. Cleaning the Injectors

We recommend thoroughly cleaning the injectors every 30 days.

1. Use a prepared solution of 70% ethanol to flush the injector(s) three times. Prepare at least 10ml of ethanol
for each injector to be flushed.

2. Place the container of 70% ethanol into the bottle holder. The inlet tube should be inserted so that it reaches
the bottom of the container.

3. Ensure the Waste Collection Tray is in position and empty.

4. From the Tools screen (SettingsTools; Tools icon at top right of screen), select “Injectors”. Tap the Prime
button to initiate priming.

5. Allow the solution to sit in the fluid path for 30 minutes before flushing the injectors.

6. From the Tools screen (SettingsTools; Tools icon in upper right corner of the title bar), select “Injectors”.
Tap the Flush button to show the Flush window.

7. Select 9 flush cycles and press Start Flush to wash the lines with 70% ethanol.

8. Empty and clean the Waste Collection Tray, and place it back into position.

9. Place a container of deionized water on the bottle holder with the inlet tube inserted to reach the bottom of
the container. Prepare at least 10ml of deionized water for each injector to be flushed.

10. From the Tools screen (SettingsTools; Tools icon in upper right corner of the title bar), select “Injectors”.
Tap the Flush button to initiate flushing.

11. Select 9 flush cycles, and press Start Flush to wash the lines with deionized water.

12. Empty and clean the Waste Collection Tray, and place it back into position.

13. Flush the injector 3 times with air. A small volume of water will remain in the injector after the air purge.

5.G. Cleaning the Waste Collection Tray

The maximum volume capacity of the Waste Collection Tray is approximately 50ml. Carefully remove the Waste
Collection Tray from its position to avoid liquid spills.

After each prime and flush sequence, discard any fluid from the Waste Collection Tray, and rinse it with deionized
water.

5.H. Removing or Replacing Inlet and Outlet Tubing

Replacement tubing assembly is available. See Section 7.E for ordering information.

1. Turn OFF the instrument. Disconnect the AC Power Adaptor.

2. Push the Microplate Sample Tray to the back of the instrument and push the Optical Head from the Home
position toward the center of the instrument (Figure 54).

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5.H. Removing or Replacing Inlet and Outlet Tubing (continued)

3. Loosen the hand screws and remove the Injector Tips and Injector Tip Holder from the Optical Head.
Facing the front of the instrument, push upward and then leftward (as viewed from the bottom of the Tip
Holder to release the Tip Holder from the Optical Head (Figure 55).

4. Remove the Injector Tubing from the Injector Tip Holder.

5. Disconnect the inlet or outlet tubing from the injector port by twisting the gray fitting counterclockwise.

6. Discard the used injector tubing.

7. Twist the fitting of the replacement tubing into the correct port of the injector pump.

Note: The inlet and outlet tubing have different lengths. Tubing with one colored band is inlet tubing

(Injector 1 is red; Injector 2 is yellow). Tubing with two colored bands is outlet tubing (Injector 1 is red;
Injector 2 is yellow).

8. Insert the free end of the injector tubing into the Injector Tip Holder until the tip of the tubing has pushed
through the red holder and stops.

9. Return the Injector Tip Holder to the Optical Head and tighten the hand screws. Position the holder just
above the front pins on the Optical Head. Push the holder toward the right to lock the holder securely to the
right pin of the Optical Head. Then push down and lock the holder on the left pin (Figure 58).

10. Insert the Injector Tips into the Injector Tip Holder. Snap the Injector Tips into place.

11. Return the Optical Head to the Home position by pushing the Optical Head from the center of the instrument
all the way to the right side of the instrument (Figure 54).

12. Pull the Microplate Sample Tray forward to the Home position.

13. Reconnect the AC Power Adaptor to the instrument. Turn ON the instrument.

5.I. Additional Maintenance

Periodically wipe off the outside of the instrument with a damp cloth. Do not use solvents or abrasive cleaners to
clean the instrument. Avoid spilling liquids into the Microplate Sample Tray.

If a spill has occurred:

1. Turn OFF the instrument. Disconnect the AC Power Adaptor.

2. Wipe up any moisture inside the Microplate Sample Tray.

3. Use Kimwipes

®

tissue dampened with 70% ethanol to clean the Microplate Sample Tray.

4. Allow the instrument to completely dry before using.

5. Connect the AC Power Adaptor and turn ON the instrument.

Note: If you suspect fluids have spilled onto the light detector, please contact Promega Technical Services for
cleaning instructions.

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TM470 · 4/16 www.promega.com

6. Troubleshooting

For questions not addressed here, please contact your local Promega Branch Office or Distributor. Contact
information available at: www.promega.com. E-mail: [e-mail]

6.A. Error Messages

Error Message Description Resolution

Start Problem Invalid Value: Selection must be

Select at least one well on the plate map.

non-empty

Protocol Validation: No plate
detected in instrument

Error: Cannot connect to the
instrument. Device is not found; is it
turned off or disconnected?

Make sure the 96-well plate was inserted and
is properly seated.

Tablet PC is not connected to the instrument,
Tablet PC is OFF or Instrument is OFF.
Check to see if the Tablet PC is connected to
the instrument or turn ON the Tablet PC or
turn ON the instrument. If the error persists,
contact Promega Technical Services.

Open door
detected during
operation

Reference
position loss

Reference
position loss
(Underrun)

The door of the instrument has been
opened unexpectedly during
operation of the instrument.

See Reference position loss
(Underrun)

During an instrument operation the
tray mechanism has not been able to
move to the desired position. This
may be caused by an obstruction in
the instrument.

Manually opening the door of the instrument
during an instrument operation will cause
that operation to be aborted.

See Reference position loss (Underrun)

Attempt to remove the obstruction manually
if possible. Press “Eject” or press “Self-Test”
in setting or press “Recovery” in the Tools
menu. Any of these methods should result in
recovery of the obstructing item. Contact
Promega Technical Services if it is not
possible to resolve the issue.

Firmware read
operation
timeout

The instrument has failed to respond
to a command.

Attempt to repeat the operation if possible. If
this error recurs please contact Promega
Technical Services to help troubleshoot the
issue.

Firmware write
operation
timeout

The instrument has failed to write
values to its firmware.

Attempt to repeat the operation if possible. If
this error recurs please contact Promega
Technical Services to help troubleshoot the
issue.

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6.A. Error Messages (continued)

Error Message Description Resolution

FPGA corrupt FPGA image programming has failed,

or FPGA module is defective.

ADC status invalid Internal hardware failure has

occurred.

Head loopback
failure

No plate in
instrument

Internal hardware failure has
occurred.

No plate is present or a clear plate is
in the Sample Tray.

Please contact Promega Technical
Services for troubleshooting assistance.

Please contact Promega Technical
Services for troubleshooting assistance.

Please contact Promega Technical
Services for troubleshooting assistance.

Use an opaque white or black plate.

6.B. Common Problems

Symptoms Causes and Comments

Injectors sputter or drip Reagent buildup in injector lines. Run the flushing
protocol (Section 4.C).

Injector speed may not be optimal for the viscosity or

composition of the solutions used.

Injectors not injecting No reagent in injector lines. Confirm that the inlet tubing is
inserted into the bottom of the reagent container.

Reprime the injectors. (Section 4.A).

Injector tubing is clogged and may need to be replaced.
(Section 5.F)

Plate not detected The Microplate Sample Tray does not contain a plate or

waste tray, as appropriate. Insert a plate tray or waste tray,
as needed.

Plate stuck or jammed in instrument Press “Eject” or press “Self-Test” in Settings or press
“Recovery” in the Tools menu (Section 3.E). Any of these
methods can be used to attempt to recover the plate. If the
issue is not resolved, contact Promega Technical
Services ([e-mail]).

Tablet PC touch screen does not 1. Verify the plug is securely connected to the Tablet PC.
appear to be working 2. Verify that the instrument is plugged into the USB
port on the Tablet PC.

3. Restart the Tablet PC, and launch the GloMax

®

Navigator software.

4. If the issue is not resolved, contact Promega Technical

Services ([e-mail]).

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TM470 · 4/16 www.promega.com

Symptoms Causes and Comments

Power failure during run In the event of a power failure, turn OFF the instrument
and the Tablet PC. When power is resumed, turn the
instrument and Tablet PC back ON. The results that were
generated during the run (before the power failure) will be
saved.

Instrument problem: “door opened” 1. Instrument door was opened during a run,
message: explanation causing the reading to abort. Restart the run.

2. Something prevented the instrument door from
fully closing. Check the instrument door for
any obstructions.

3. The door sensor may be bent or broken. Contact
Promega Technical Services.

Luminescent signal is reported as SAT The luminescent output of the sample is saturating the
detector.

1. Use less sample.

2. Use a black plate.

Low luminescent signal Low activity in sample.

Use white opaque plates.

High luminescent cross talk Use high-quality white or black opaque plate.

Injector leaks The inlet or outlet tubing is not properly connected to the

injector pump. See Section 5.H for instructions on
removing and replacing tubing.

Injectors do not sit properly in the Reagent residue has built up inside the Injector Tip Holder.
Injector Tip Holder See Section 5.D for instructions on cleaning the Optical

Head.

Grinding noise during run Reagent residue has built up on the Aperture. See

Section 5.E for instructions on cleaning the interior of the
instrument.

The Microplate Sample Tray cover is open during the run.

Close the tray cover and secure with the tray latch. The
plate holder should be in the Home position.

The Microplate Sample Tray cover does not close The 96-well plate is not sitting flat in the Microplate

Sample Tray. Gently push the tray cover up to a 90 degree
angle with the Microplate Sample Tray to completely open
the tray. Place the 96-well plate inside the tray.

Remove lid from 96-well plate, place inside the Microplate

Sample Tray and close the tray cover.

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6.B. Common Problems (continued)

Symptoms Causes and Comments

Moisture appears on top of the Microplate Sample The injector tips are bent. See Sections 5.D and 5.I for
Tray cover after a run. instructions to change the tubing and clean the Optical

Head.

Injector tip holder is not seated correctly. Injector speed is

not set correctly, causing reagent drips.

The 96-well plate has overflowed inside instrument. • See Section 5.D for instructions to clean the Optical

Head.

• Check the wells selected for injection and measurement.

• Check the total injection volume in the software protocol.

• Check the volume of sample inside the well. The
maximum volume per well is 300µl.

• Thoroughly clean the interior of the instrument.

Reagent has spilled inside the instrument Wipe up spills immediately using an absorbent paper towel

or Kimwipes

®

tissue. If necessary, use a 70% ethanol

solution to remove reagent residue.

6.C. Use of a USB Flash Drive

• Insert a USB flash drive into one of the two USB ports on the back of the instrument before running any proto-

col and do not remove until the protocol is finished.

• Do not insert or remove USB flash drive while instrument is running.

• Because USB flash drives may vary from vendor to vendor or from type to type, incompatibilities may occur.

If the USB flash drive is not detected after a few seconds or if you encounter any issues with the USB flash
drive, shut down and restart the Tablet PC and the instrument, and try a different brand of USB flash drive.

• If the Tablet PC becomes unresponsive after inserting a USB flash drive, shut down the Tablet PC and

instrument, remove the USB flash drive, and restart the Tablet PC and the instrument.

48 Promega Corporaon · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax [phone]

TM470 · 4/16 www.promega.com

7. Appendix

7.A. Configuring the Tablet PC

The GloMax

®

Navigator Tablet PC touch screen allows the user to adjust various operating system configurations

and user settings as described below.

Your IT department or site Administrator should configure the Tablet PC according to the IT rules and IT procedures
pertinent to your institution. The following is a set of guidelines for your IT personnel or site Administrator.
Depending on your institution’s IT policy, you may or may not be able to configure/change the settings for the Tablet
PC. The Tablet is pre-installed with a Microsoft Excel

®

reader, but you may install the full version of Microsoft Office
if you desire. We do not recommend loading other programs onto the Tablet because these may interfere with the
instrument operation.

Note: Some institutions require anti-virus software installed on PCs within the institution. If installing an

!

antivirus program please ensure it is set to manual, not automatic, update.

Antivirus software will slow the performance of the Tablet PC and will affect the performance of the GloMax
Navigator Instrument while it is measuring samples.

Additional recommendations if using your own PC:

• Disable automatic Windows

®

updates and virus scans

• Disable automatic power off, sleep, or hibernation settings

• Disable “Fast Startup” in Windows

®

8. Set the power button to Shut Down not Sleep.

• Disable any other automatically or manually started software that would consume PC resources during

operation.

®

• Ensure there are no domain policies that would overwrite any of the above settings.

• Ensure that Windows

Note: The Tablet PC that is provided with GloMax

®

will not turn off USB ports to save power.

®

Navigator System has already been configured for the settings

listed above.

The first time you use the GloMax

®

Navigator Instrument and Tablet PC you should check the configuration of the

following options. Settings can be changed later if required by following the instructions again.

1. Touch Screen

The GloMax

®

Navigator Tablet PC has a touch screen that can be used to navigate to the various screens. Touching

and holding an icon on the Tablet for 3 seconds is equivalent to a Right Click on a normal PC.

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2. Date and Time

The date and time set on the Tablet PC are used for the instrument run log to indicate when a protocol was run on
the GloMax

a. Select the Windows

®

Navigator Instrument, as well as during kinetic runs to mark the time during each read.

®

8 “Settings” charm by swiping in from the right screen edge. Select Control Panel (or

type “control panel” in the Search tool if you do not see Control Panel).

b. In the Control Panel menu, tap the Date and Time menu item.

Note: If you are unable to see the Date and Time menu item, tap Category View in the upper right corner of

the window and choose either Large or Small icons.

c. First set your time zone by tapping the Change Time Zone button.

d. On the Time Zone Settings window, select the appropriate time zone for your location and whether you wish

the clock to automatically adjust for Daylight Savings Time.

e. Tap the OK button to save the time zone settings.

f. You will be returned to the Date and Time Settings window.

g. On the Date and Time Settings window tap the Change Date and Time button.

h. Set the appropriate date and time for your current location using the calendar and clock setting icons.

i. Tap the OK button to save the date and time settings.

j. You will be returned to the Date and Time Settings window. Tap the OK button on this window to return to

the Control Panel.

k. Close the Control Panel by tapping the red X button in the upper right-hand corner of the screen.

3. User Password and Setup

®

GloMax

Navigator software supports the following user roles.

PromegaAdministrator: Promega Administrators have the following capabilities:

• All PromegaUser capabilities

• Import protocols

• Select Export folders

• Set Instrument Name

• Export Audit Records

PromegaUser: Promega Users have the following capabilities:

• Select and run preloaded protocols

• Build user-defined protocols

• View, export results

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Assigning User Accounts to Promega Groups

The GloMax

®

Navigator Software employs the Windows® login user access levels as a security tool for adding
approved users and associated passwords. New user accounts should be created based on policies governing your
institution. Only someone with an Administrator account can assign users to Promega groups. You must close the

®

GloMax

Navigator Software to create user accounts.

1. Swipe in from the right edge of the screen and select Search, then type in “lusrmgr.msc” and press Enter.

The Local Users and Groups dialog should appear.

2. In the left-hand pane of the Computer Management window, select Local Users and Groups and double-tap

Groups.

Note: If the Local Users and Groups menu item is not visible, select the System Tools item to expand that

menu heading.

Figure 58. Selecting Groups from the Computer Management screen.

4. If you want to add Administrators, double-tap PromegaAdministrators. A ‘PromegaAdministrator’ window

will open, which can be used to add existing users to the PromegaAdministrators group. Tap the Add…
button to add users to the PromegaAdministrators group. In the “Enter the object names to select” area of
the window enter the username of the user you wish to add to this group. Tap OK on this window and tap
OK on the PromegaAdministrator Properties window to add users to the PromegaAdministrators group.
Close the Computer Management window.

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7.A. Configuring the Tablet PC (continued)

3. User Password and Setup (continued)

Figure 59. Adding users to the PromegaAdministrators group.

5. If you want to add users, tap PromegaUsers. A PromegaUsers window will open, which can be used to add
existing users to the PromegaUsers group. Tap the Add… button to add users to the PromegaUsers group. In
the “Enter the object names to select” area of the window enter the username of the user you wish to add to
this group. Tap OK on this window, and tap OK on the PromegaUsers Properties window to add users to the
PromegaUsers group. Close the Computer Management window.

Figure 60. Adding users to the PromegaUsers group.

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7.A. Configuring the Tablet PC (continued)

3. User Password and Setup (continued)

Removing User Accounts

The GloMax

®

Navigator Software employs the Windows® login user access levels as a security tool for adding
approved users and associated passwords. Removing existing users can only be performed by someone with
Administrator privileges.

1. Swipe in from the right edge of the screen and select Search, then type in “lusrmgr.msc” and press Enter.

The Local Users and Groups dialog should appear.

2. Tap Local Users and Groups and double-tap Groups.

3. If you want to remove Administrators, tap PromegaAdministrators. A ‘PromegaAdministrator’ window will

open, which can be used to remove existing users from the PromegaAdministrators group.

4. Select the user you want to remove and tap the Remove… button. Tap OK to remove selected user from the

PromegaAdministrators group. Close the Computer Management window.

5. If you want to remove users, tap PromegaUsers. A PromegaUsers window will open, which can be used to

remove users from the PromegaUsers group. Select the user to be removed and tap the Remove… button
Touch OK to remove the selected user from the PromegaUsers group.

Set Instrument Name (GloMax

Administrators can set the instrument name using the GloMax

®

Navigator Software)

®

Navigator Software by tapping the instrument
name button from the Administrator Page window (Figure 80, Home  Settings  Administrator). Administrators
can use the on-screen keyboard to manually enter the instrument name (Figure 62). The instrument name should
be entered following the procedures and rules at your site.

Note: Changing the instrument name will force a reboot of the Tablet PC.

!

13624TA

Figure 61. GloMax® Navigator Software Administrator screen.

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www.promega.com TM470 · 4/16

12724T

A

11609TA

13629TA

13630TA

11616TA

Figure 62. Instrument Name window. Administrators can manually enter the instrument name from the
Instrument Name Window.

7.B. Icons and Symbols

Home screen

Exit Software

Quick access to the Tools Screen

Home all axes

Help Screen

From any screen other than the Home screen

Home. Pressing this icon will take you to the Home Screen.

Protocol screen

Favorite. Mark to select a favorite protocol, which will then sort to the top of the page.

Yellow favorite indicates selection.

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TM470 · 4/16 www.promega.com

Protocol Editor screen

11617TA

11620TA

11621TA

Plate map is locked and applied to every step within the protocol

Plate map is unlocked, and each step of the protocol may have a different plate map selection.

Plate map

Attention, there is an error in the protocol. Touch this icon for information.

Results screen

Press to turn the heat map display “on” or “off.

Delete a result file from the Results Screen.

7.C. Warranty and Service

The GloMax

®

Navigator System comes with a one-year warranty from Promega. Additional warranty and service
agreements are available. For more information, contact Promega Technical Services. Contact information is
available at: www.promega.com. E-mail: [e-mail]

To obtain service during the warranty period, please take the following steps:

1. Contact Promega Technical Services.

2. Carry out minor adjustments or tests as suggested by your Technical Services contact.

3. If it is determined that the instrument should be returned for repair, Promega Technical Services will arrange

for service by an authorized GloMax

®

Service Agent. You will be issued a Promega return authorization
number. YOU MUST OBTAIN a Promega return authorization number (RMA number) before returning an
instrument for service.

4. Before returning the instrument, you will be responsible for cleaning it and providing a Certificate of
Decontamination (see Section 7.D). If the instrument has been exposed to any chemical, biological or
radioactive hazards, contact Promega Technical Services for decontamination instructions before shipping.

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7.D. Certificate of Decontamination

Disinfection and decontamination are required before shipping the instrument and instrument accessories for repair.
Instruments returned for service must be accompanied by a signed and dated Certificate of Decontamination, which
must be attached to the outside packaging of the instrument. Print this page, fill out, sign and return along with the
instrument for repair.

1 Clean the interior of the instrument according to the instructions in Section 5.E.

2. Clean the injectors according to the instructions in Section 5.F.

3. Clean the Waste Collection Tray according to the instructions in Section 5.G.

Failure to confirm disinfection and decontamination will result in decontamination charges before the instrument
will be serviced.

Select either (A) or (B):

A. I confirm that the returned items have not been used with body fluids or contaminated by toxic, carcino-

genic, radioactive or other hazardous materials.

B. I confirm that the returned items have been decontaminated and can be handled without exposing personnel

to health hazards.

Circle the type of material used in the instrument:

Chemical Biological Radioactive**

Briefly describe the decontamination procedure performed: ___________________________________

____________________________________________________________________________
____________________________________________________________________________

____________________________________________________________________________

Date: ____________________________________

Place: ____________________________________

Signature: _________________________________

Name (block capital letters): _______________________________________________________

** The signature of a Radiation Safety Officer is also required if the instrument was used with radioactive materials.

This instrument is certified by the undersigned to be free of radioactive contamination.

Date: ____________________________________

Place: ____________________________________

Signature: _________________________________

Name (block capital letters): _______________________________________________________

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TM470 · 4/16 www.promega.com

7.E. Related Products

GloMax

®

Navigator System Accessories

Product Size Cat.#

®

Dual Injectors and Pump Station Upgrade for GloMax

®

GloMax

Replacement Tubing Assembly for GloMax

GloMax

Systems Light Plate 1 each E5141

®

Navigator (Set of 2) 1 each GM2030

®

Navigator System Service Options

Navigator 1 each SA1304

Product Size Cat.#

®

GloMax

GloMax

GloMax

GloMax

GloMax

GloMax

GloMax

Navigator Standard Service Agreement 1 each SA1301

®

Navigator Parts Only Service Agreement 1 each SA1302

®

Navigator Basic Service Agreement 1 each SA1303

®

Navigator Preventive Maintenance 1 each SA1308

®

Navigator Installation Qualification (IQ) 1 each SA1305

®

Navigator Operational Qualification (OQ) 1 each SA1306

®

Navigator Installation and Operational Qualification (IQ/OQ) 1 each SA1307

Cell Viability, Cytotoxicity and Cell Health Assays

Product Size Cat.#

®

CellTiter-Glo

Luminescent Cell Viability Assay* 10ml G7570

BacTiter-Glo™ Microbial Cell Viability Assay* 10ml G8230

Mitochondrial ToxGlo™ Assay* 10ml G8000

CytoTox-Glo™ Cytotoxicity Assay* 10ml G9290

*Additional Sizes Available.

Apoptosis Assays

Product Size Cat.#

®

Caspase-Glo

3/7 Assay* 10ml G8091

*Additional Sizes Available.

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Reporter Assays

Product Size Cat.#

®

Dual-Glo

Dual-Luciferase

Luciferase Assay System* 10ml E2920

®

Luciferase Reporter Assay System* 100 assays E1910

Luciferase Assay System* 100 assays E1500

NanoBiT™ PPI MCS Starter System 1 each N2014

®

NanoBiT™ PPI Flexi

®

Nano-Glo

Nano-Glo

Nano-Glo

Live Cell Assay System* 100 assays N2011

®

Luciferase Assay System* 100ml N1120

®

Dual-Luciferase® Reporter Assay System* 100ml N1620

Starter System 1 each N2015

ONE-Glo™ Luciferase Assay System* 10ml E6110

™

Bright-Glo

Steady-Glo

Luciferase Assay System* 10ml E2610

®

Luciferase Assay System* 10ml E2510

Renilla Luciferase Assay System* 100 assays E2810

®

Renilla-Glo

Luciferase Assay System* 10ml E2710

ADCC Reporter Bioassay, Core Kit 1 each G7010

ADCC Reporter Bioassay, Core Kit 5X 1 each G7018

ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay* 1 plate E7110

*Additional Sizes Available

Cell Signaling Assays

Product Size Cat.#

P450-Glo™ CYP1A2 Screening System 1,000 assays V9770

P450-Glo™ CYP2C9 Screening System 1,000 assays V9790

P450-Glo™ CYP3A4 Screening System 1,000 assays V9800

P450-Glo™ CYP2C19 Screening System 1,000 assays V9880

P450-Glo™ CYP2D6 Screening System 1,000 assays V9890

ADP-Glo™ Kinase Assay* 1,000 assays V9101

®

Kinase-Glo

Kinase-Glo

Kinase-Glo

Luminescent Kinase Assay* 10ml V6711

®

Max Luminescent Kinase Assay* 10ml V6071

®

Plus Luminescent Kinase Assay* 10ml V3771

cAMP-Glo™ Assay* 300 assays (384-well plate) V1501

GloSensor™ cAMP Reagent* 25mg E1290

*Additional Sizes Available.

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(a)

Patent Pending.

© 2016 Promega Corporaon. All Rights Reserved.

Bright-Glo, Caspase-Glo, CellTiter-Glo, Dual-Glo, Dual-Luciferase, GloMax, Kinase-Glo, Nano-Glo, Renilla-Glo and SteadyGlo are a registered
trademarks of Promega Corporaon.

ADP-Glo, BacTiter-Glo, cAMP-Glo, CytoTox-Glo, GloSensor, NanoBiT, ONE-Glo, P450-Glo and ToxGlo are trademarks of Promega Corporaon.

Windows is a registered trademarks of Microso Corporaon. Kimwipes is a registered trademark of Kimberly-Clark Corporaon. Paralm is a
registered trademark of American Naonal Can Company.

Products may be covered by pending or issued patents or may have certain limitaons. Please visit our Web site for more informaon.

All prices and specicaons are subject to change without noce.

Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date
informaon on Promega products.

Promega Corporaon · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax [phone]

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