Primerdesign genesig q16 Instruction Manual

The genesig® q16

Instruction
Manual

The genesig

®

q16

instruction manual

Congratulations on purchasing the
genesig q16. The q16 is a revolutionary
instrument that brings the 21st century
technology of DNA testing to everyone.
As well as being beautiful and highly
portable, the q16 is powerful. In
combination with the genesig Easy kit
range the genesig q16 can be used to
detect and quantify over 450 different
DNA/RNA targets.

2

1|

Connecting the machine and
installing the software 04

2|

General operation
of the instrument 08

3|

Opening the software and
selecting a test type 10

4|

The main screen
layout 12

5|

Results
24

6|

Exporting
results 30

7|

What do my
results mean? 32

8|

Troubleshooting
40

3

Section 1

Unpack your genesig q16 and remove all packaging.
Insert the power cable in to the power jack in the base of
the instrument and plug into a power source.

The q16 can be operated in three ways.

If you are planning to run the instrument via a direct
connection to Mac/PC, plug in the Ethernet cable to the
base of the instrument and connect to a spare Ethernet
port on your Mac/PC. Ideally your Mac/PC should run
the most recent version of the operating system. The
genesig q16 will run on OS X version 10.7.3 and newer
releases for Mac and Windows Vista or 7, 8, 8.1 and 10
for PC.

If you have multiple genesig q16 instruments you can
connect them all to the same Mac/PC via a network
switch.

Direct connection to Mac/PC

If you are planning to run the instrument via a local area
network, plug in the Ethernet cable to the base of the
instrument and connect to a spare Ethernet port on your
network.

Direct connection to a local area network

Connecting the machine

4

Section 1

If you are planning to run the instrument in standalone
mode no Ethernet cable connection is required. Instead
a USB drive is loaded with the experiment setup data
and then inserted in to the base of the q16 via an
extension cable, which is supplied, to initiate a run.

Please ensure that you use the USB drive provided with
your instrument.

If using a USB drive from an alternative supplier the
strict minimum speed limit for compatibility with the
q16 software is 0.2MiB/s average over 10MiB of data (a
10MiB le should be written in 50 seconds or less).

Standalone operation via a USB drive

5

Section 1

Installation of software

The software requires a JavaScript runtime environment
to operate successfully. If your PC/Mac does not
currently support a current Java run-time environment
you will be prompted to download and install an update.
Follow the onscreen instructions to download and install
the update and then re-start your computer.

The q16 software is provided on the USB drive
shipped with your instrument. The latest software is
always available from http://www.genesig.com/q16­software. Open the installer le and follow the onscreen
instructions.

6

Section 1

7

General operation
of the instrument

Section 2

To open the q16 twist the lid a quarter turn anti­clockwise and lift. The lid is replaced by repeating this
operation in reverse.

The genesig q16 uses low prole 0.2ml reaction tubes
that are supplied as part of each genesig EASY kit.

Do not use alternative reaction tubes as this may
damage your instrument.

NEVER open a genesig q16 reaction tube after an
analysis run has completed. Dispose of them carefully

and ensure that they do not open by accident, as this
risks contaminating your working area and may affect
future test results.

8

Section 2

9

Section

Open the software

Section 3

Once installed double click on the q16 software icon to
open the software.

Click the ‘New’ button to begin a new test.

10

Section

Selecting a test type

Section 3

Select the type of test you wish to perform:

Species test

Select this if you wish to use a genesig EASY speciation
kit. This test will calculate the percentage of your sample
that is a particular species e.g. you can calculate what
percentage of your beefburger is horse meat.

Target detection test

Select this if you are using a genesig EASY kit for the
detection of a particular target e.g. you can test for the
presence of Salmonella, HIV or FMDV. This test will also
approximate the quantity of the target DNA present.

11

Section

The main screen layout

Section 4

There are 2 tabs in the view. One for setting up your test
and a second where your results will be displayed after
the analysis is complete:

In the setup screen there are 5 simple panels to consider;

1. Notes:

12

SectionSection 4

Your test will be given a default name but this can be
changed by clicking in the name eld and entering new
text. The Notes eld allows you to make notes on your
test if required.

2. Samples:

13

Section

The main screen layout

Section 4

In this panel you can dene how many samples you
wish to test. Use the + button to add more, and the –
button to remove them. You can alter the order of the
samples using the up and down arrows. The software
automatically limits the number of samples you can have
dependent on the capacity of the instrument.

You can alter the name of your samples by clicking in the
“Name” eld and adding new text. You can also attach
more detailed notes to a sample by clicking in the notes
eld and adding text.

Samples are coloured to make visual identication
easier. You can alter the colour associated with each
sample by double-clicking on the coloured square next
to it.

3. Tests:

2. Samples cont...

14