postnova HRFFF User Manual
HRFFF 10.000
非对称场流分离色谱
德国制造
HRFFF 10.000
Asymmetric
Asymmetric
Flow Field-Flow Fractionation
Flow Field-Flow Fractionation
advanced analytical products
Characterization of
Characterization of
Bio-/Polymers, Proteins
Bio-/Polymers, Proteins
and Nanop
and Nanop
www.postnova.com
articles
articles
Specifications
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General sample size and molar mass range:
Particles: 1 nm to 100 µm
Polymers: 10
Analysis time:
1 to 60 min, typically 15 - 20 min
Channel dimensions:
Volume : between 0,5 - 2,5 mL
Size : 335 x 60 x 40 mm
Thickness: 350 or 500 µm
Carrier liquids:
Aqueous : any aqueous liquid, pH from 2 - 11,
ionic strength from d.i.water to saline
Organic : THF, MeOH, etc.
Membranes:
Broad range of membranes available (reg.
cellulose, polycarbonate, PES, etc.);
available sizes 0,3; 1; 2; 4; 5; 10; 30; 100 kDa
respectively 10; 30; 50 nm or larger.
Detectors:
UV, RI, laser light scattering, fluorescence detection,
and more; multiple detectors in line possible.
Laminar flow range:
0 - 10 mL/min
Cross flow range:
0 - 10 mL/min
Pressure limit:
0 - 31 bar
Operating temperature:
5 - 45°C
Power requirements:
110V or 240V, 50/60 Hz; 2,5 A for basic set-up.
PC Requirements:
Windows, min. 32 MB RAM.
System software:
NovaFFF Control, NovaFFF Analyze
compatible with NovaGPC
Maintenance:
Consumables: piston seals, inline filters,
membranes.
Injection volume:
1 - 1000 µL
Standard 20 µL
optional > 1000 µL
Injected sample mass:
Up to 500 µg and more; depending on sample
characteristics and channel version (thickness);
Typical injection mass 20 - 100 µg
Injection method:
Via Rheodyne hand injections valve or automated
via PN5200 autosampler with different options e.g.
to cool samples (standard vial, microwell plates
and other sizes are available).
3
Da to 1012 Da.
post
The HRFFF 10.000 Series
is working on the basis of Asymmetric Flow Field-Flow Fractionation,
a new and efficient separation method to fractionate and characterize
particles and bio(polymers) in fluids fast and gently. The system has
a modular set-up with different pumps, detectors, autosamplers etc.
available. The 10.000 - Series system is able to separate samples
according to their size and to determine molar mass, R
g and Rh of
the separated analytes on-line utilizing different laser light scattering
systems on the basis of static and dynamic laser light scattering
(SLS/DLS).
nova
The system has a large, nearly universal separation range. Particles
can be separated from 1 nm up to 100 µm and polymers from 10
to 1012 Da. The fast and gentle separation occurs according to the
diffusion coefficient in a patented laminar flow channel without using
a stationary phase as in chromatography.
Applications of the 10.000 - Series
analytics
Biotechnology : cells, aggregates, organelles, viruses particles, etc.
Pharmaceutical : proteins, antibodies, liposomes, micelles, etc.
Environment Science : environmental particles, humic, clay colloids, etc.
Material Science : coatings, nanoparticles and high-tech materials, etc.
0,3
0,25
0,2
0,15
0,1
Signal Intensity (UV; 210 nm) / a.u.
0,05
0
0 200 400 600 800 1000 1200
Fig. 1: Fractogram of a latex particle mixture separation with the HRFFF 10.000
21 nm
Advantages of the HRFFF 10.000
51 nm
100 nm
200 nm
Retention Time / s
3
· Fast and gentle separation without a stationary phase.
· Direct sample injection, no sample preparation steps neccessary.
· On-channel concentration of diluted samples during injection.
· Flexible separation conditions, aqueous and organic solvents.
Large and easily accessable mass and size range.
·
· Fast separation of complex polymer, particle and colloid systems.
· Low shear forces during separation protect bioactivity.
· Patented separation channel for fast exchange of membrane.
· Professional and integrated system from one manufacturer.
New bench space saving module design and construction.
·
Completely automated system by using an autosampler and
·
total system control using the NovaFFF windows software.
· Various additional postnova analytics modules and components
available: autosamplers, degassers, piston/syringe pumps etc.
· Dynamic (DLS) and static (SLS) laser light scattering in one
integrated detector with flow-cell.
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