PolySciences 3025 series Instruction Manual

3025 MICROSCOPE SERIES

INSTRUCTIONS

Polysciences, Inc.

400 Valley Road
Warrington, PA 18976

[phone] / [phone]
[phone] / [phone] fax
[e-mail]
www.polysciences.com

Our catalog lists over 3,000 unique and specialty products that are used by histology professionals, lab technicians,
pathologists, chemists and scientists worldwide. Visit www.polysciences.com to learn more about our product lines.

Polysciences Europe GmbH

Handelsstrasse 3
D-69214 Eppelheim
Germany

(49) 6221-765767
(49) 6221-764620 fax
[e-mail]

Asia-Pacific Headquarters

Polysciences Asia Pacific, Inc.
2F-1, 207 DunHua N. Rd.
Taipei, Taiwan 10595

(886) 2 8712 0600
(886) 2 8712 2677 fax
[e-mail]

TABLE OF CONTENTS

Safety notes……………………………………………………………… i

Care and Maintenance…………………………………………………. ii

1. Components Illustration………………………….…………..………… 1

2. Installation of Components…………………………….………………. 4

2.1 Installation Diagram………………………………………………………...… 4

2.2 Installation Procedures…………………………………………………………5

3. Operation………………………………………………….……………..11

4. Technical Specifications……………………………...….…………….. 15

5. Trouble Shooting Guide.......................................................................... 16

SAFETY NOTES

be sure the main switch is off (“O”), remove

1. Open the shipping carton carefully to prevent any accessory, i.e. objectives or eyepieces, from

dropping and being damaged.

2. Do not discard the molded Styrofoam container; the container should be retained should the

microscope ever require reshipment.

3. Keep the instrument out of direct sunlight, high temperature or humidity, and dusty environments.

Ensure the microscope is located on a smooth, level and firm surface.

4. If any specimen solutions or other liquids splash onto the stage, objective or any other component,

disconnect the power cord immediately and wipe up the spillage. Otherwise, the instrument may

be damaged.

5. CAUTION: the lamp, lamp housing and adjacent parts will become very hot. Do not touch these

parts until they have completely cooled. Never attempt to handle a hot halogen bulb.

6. All electrical connectors (power cord) should be inserted into an electrical surge suppressor to

prevent damage due to voltage fluctuations.

7. For safety when replacing the halogen lamp or fuse,

the power cord, and replace the halogen bulb after the bulb and the lamp house has completely

cooled.

8. Confirm that the input voltage indicated on your microscope corresponds to your line voltage. The

use of a di fferent input vol tage than indicated will cause severe damage to the microscope.

i

COMPONENTS ILLUSTRATION

1

2

⑥

Video Port (optional)

⑤

10×Wide Field Eyepiece

④

Infinity Plan Objectives

①

②

Microscope Body

Trinocular Viewing Head

③

Mechanical Stage Mounting Assembly

3

Lamp House

2-2 Assembly

3025

Guide Board

Figure 1

Locking Block and Bolt

Figure 2

Fig ure 3

4

2-2-1 Installing the Mechanical Stage Mounting Assembly

1. Before installing the mechanical stage mounting device, be sure to adjust the coarse focus

knob until the guide board (Figure 1) is at its lowest position. This allows you to install

the mechanical stage easily. Use the 3.0mm hexagonal Allen wrench to connect the

mechanical stage mounting support assembly and the guide board. Ensure the connection

is tight and secure.

2. Place the mechanical stage mechanical assembly (Figure 2) onto the top of the guide

(Figure 1). Ensure the assembly (Figure 2) is completely attached to the guide board before

board

tightening the allen screw.

The mechanical stage has been factory assembled and adjusted. Disassembly of the

mechanical stage should be attempted only by a trained microscope service

technician.

5

: Do not use a

the power cord.

3025

2-2-2 Installing the Trinocular Viewing Head

Insert the trinocular viewing head (Fig.4) into the

Figure 4

microscope body (Fig. 5); then rotate the head into

its proper viewing position. Use the 2.5mm

hexagonal wrench to attach the viewing head to the

body by tightening the allen screw. Ensure the head

Bolt

is securely attached.

Figure 5

2-2-3 Installing and Replacing the Lamp

(Figure 6)

Halogen Lamp: 6 Volt 30 Watt
lamp of a different voltage or wattage.

1．Turn the power switch to the off position and remove

Bolt

Lamp House Bolt

Figure 6

2. Allow the lamp house and bulb to completely cool.

Remove the lamp housing by pulling gently.

Remove the old lamp from the socket and replace

with a 6V30W halogen lamp.

Do not touch the halogen lamp with your bare

fingers. Doing so will shorten the service life of the

lamp. Use a soft, clean cloth or lint free paper tissue.

6

3025

2-2-4 Installing the Lamp House

Jack

Figure 7

Align the lamp house bolt (Fig. 6)
with the jack on the back of the
microscope (Fig.7), then gently push the
lamp holder into the housing until they
are completely against each other
(Fig. 8).

2-2-5 Installing the Objectives

Figure 8

Figure 9

1. Rotate the coarse focus knob until the

mechanical stage is at its lowest position.

2. Install the lowest magnification objective into the

nosepiece (Fig. 9). Then in a clock-wise

direction rotate the nosepiece and install the

objectives in succeeding higher magnification

sequence (Fig. 10).



Inspect the objectives regularly for dust, dirt

and oil. Clean the objectives according to the

directions in the “Care and Maintenance”

section.

Use the 10x objective to initially focus the



image of your specimen.



To change objectives, rotate the nosepiece

until you hear a “click” sound. This ensures

the objective is centered in the optical light

path.

Figure 10

7

Eyepiece

3025

2-2-6 Installing the Eyepieces

Fig. 12 Fig. 11

Thumb

Screw

Remove the pr otective caps from t he

eyepiece tubes. Insert the eyepieces into the

eyepiece tubes (Fig. 11).

2-2-7Installing the Video Port (optional)

Remove the protective cap from the

Figure 13

vertical tube of the viewing head. Insert

the video port (Fig. 12) into the trinocular

viewing head (Fig. 13), then tighten the

thumb screw.

Figure 14

8

Condenser Adjustment

Knob

Upper Stage

Limit Control

Fine Focus

Knob

Field Diaphragm

Coarse Focus

Knob

9

Interpupillary Distance

Intensity

Indicator

Swing Out Condenser

with Iris Diaphragm

Y Stage Movement

Adjustment Knob

Tension Control

Collar

Variable Light

Adjustment Dial

X Stage Movement
Adjustment Knob

10

3025

Figure 15

Main switch

4-1 Turning on the Lamp (Figure 15)

Connect the power cord, turn on the main switch (Figur e

15) to the on position “--”

NOTE: The switch adjacent to the main switch is

non-functioning.

4-2 Adjusting Illumination Intensity (Figure 16)

Adjust the illumination intensity by rotating the variable

intensity dial.

Figure 16

 Use of the lamp at a lower intensity will prolong its

life.

4-3Adjusting Tension Adjustment Collar

(Figure 17)

 The tightness of the tension adjustment collar is

factory adjusted. If the collar loosens or the

mechanical stage drops by itself, adjust the tension

adjustment collar until the proper tension is restored.

Tension Adjustment Collar

Figure 17

Use the black adjusting wrench supplied with the

microscope to adjust the tension adjustment collar

11

3025

Turn the X and/or Y

F

igure 19

Figure 18

Diopter Collar

Figure 20

4444----4444 Placement of Specimen (Fig. 18)

Place the slide on the mechanical stage. Use the slide

holder to gently secure the slide.

stage movement adjustment knobs to position the
specimen.

 Use caution when changing objectives. Do not

allow an objective to touch a specimen slide. Doing so

may damage the objective and specimen.

4-5 Adjusting the Interpupillary Distance

(Figure 19)

The interpupillary distance range: 48mm～75mm.

While observing with two eyes, hold the left and right

eyetubes. Rotate the eyetubes around the central axis.

Adjust the interpupillary distance until the left and right

fields of vi ew coincide completely with one image.

4-6 Adjusting the Diopter (Figure 20)

1. Using the 1 0x objective and your right eye
only, observe your specimen through the right
eyepiece only and bring it into focus.

2. Then observe the specimen with your left eye
only through the left eyepiece. If the specimen
is not in focus, rotate the diopter collar (Fig.20)
until a sharp image is obtained.

The diopter range is ±5

12

3025

nt of the numerical aperture, not for brightness.

Swing Out Condenser

Figure 23

Figure 21

Figure 22

Aperture Diaphragm

Adjusting the Aperture Diaphragm

The aperture diaphragm is designed for the adjustme

Generally, reducing the diaphragm opening to 70-80% of the N.A. value of the respective objecti ve

will provide an image of acceptabl e quality. If you want to observe the image of the apertur e

diaphragm, remove one eyepiece and look through the tube. You will see a dark circle encroaching on

the bottom of the tube

4-7 Focusing (Figures 21 & 22)

Push the light path selector lever (Figure 25) completely in.

Lower the mechanical stage. With the 10x obj ective in

position, raise the mechanical stage slowly using the coarse

focusing knob until the specimen is in focus. Then rotate

the fine focus until a sharp image is obtained. Do not allow

the objective to touch the specimen.

4444----8888 Adjusting the Swing Out Condenser
(Figure 23)

The center of the condenser and the light axis of the

objective are coaxial. They have been factory adjusted

and does not need to be adjusted . The upper limit of

the condenser has also been adjusted.

Turn the condenser focus knob to adjust the
condenser. Raise the condenser when using the
higher magnification objectives, and lower the
condenser when using lower magnification
objectives.

Swing out the condenser and away from the

light path when using the 4x objective. Swing

in the condenser and into the light path when

using the 10x and higher magnification

objectives.

13

14

Specifications 3025

Main specifications

Optical System

Viewing Head

Eyepiece (Ocular) Extra Wide Field EW10X/22, tube30 matched

Nosepiece

Objectives Infinity Plan Achromat: 4x, 10x, 40x, 100x Oil

Focus System

Stage Double plate mechanical stage; 185 x 142mm; movement range: 75 x 55mm

Koehler Illumination Koehler illumination system, Aspheric collector, halogen lamp 6V30W

Condenser Swing out condenser N.A. 0.9

Configuration Table

Viewing Head Compensation Free Trinocular Head 

Eyepiece Extra Wide Field Eyepieces: EW10x/22 

Objectives

Condenser Swing out Condenser N.A. 0.9/0.25 

Video Accessories 

Video Mount

Polarization

Turret Phase Contrast

Condenser

Darkfield Condenser

Fluorescent

Attachment

Temperature Control

Device

Note: ●Standard ○ Optional

Objective Specifications

Objective Specifications

Objective SpecificationsObjective Specifications

Magnification

4X 0.10 25.42 0.17  Red

10X 0.25 11 0.17  Yell ow

40X 0.65 0.75 0.17  Blue

100X 1.25 0.21 0.17  Black and White

Infinit y Optical System

Compensation Free Trinocular Head, Inclined 30º; Diopter ±5

Interpupillar y distance: 48-75mm

Reversed Quintuple Nosepiece

Coaxial Coarse and Fine Focusing System

Sensitivity and Graduation of Fine Focus: 0.001mm

Infinite Plan objectives: 4x, 10x, 40x, 100x Oil 

Infinite Plan Objective: 20x 

C-Mount 1x 

C-Mount 0.5x 

Numerical Value

Aperture

Diaphragm

(N.A.)

Working

Distance

(mm)

Thickness of

Cover Slip

(mm)

Conjugate

Distance

(mm)

○

○

○

○

○

Objective Color

Coding

15

16

IMAGE PROBLEMS

Problem Cause Corrective Measure

Image tinged yellow Lamp intensity is too low Adjust the light intensity by
rotating the intensity control
dial

Image is too bright Lamp intensity is too high Adjust the light intensity by
rotating the intensity control
dial

Insufficient brightness Lamp intensity is too low Adjust the light intensity by
rotating the intensity control
dial

Aperture diaphragm closed Open to the proper setting
too far

Condenser position too low Position the condenser at
the upper limit

MECHANICAL PROBLEMS

Image will not focus with Slide upside down Turn the slide over so the
high power objectives cover glass faces up

Cover glass is to thick Use a 0.17mm cover glass

High power objective contacts Slide upside down Turn the slide over so the
slide when changed from cover glass faces up
low power objective
Cover glass is to thick Use a 0.17mm cover glass

Diopter adjustment is not set Readjust the diopter settings
properly

Lamp does not light when No electrical power Check power cord connection
switched on
Lamp bulb burnt out Replace bulb

Fuse blown out Replace fuse

Slippage of focus when using Tension adjustment is set too Increase the tension on the
the coarse focusing knob low focusing knobs

Fine focus is ineffective Tension adjustment is set too Loosen the tension on the
high focusing knobs

17

ORDERING INFORMATION

Cat. #

25097

Description

3025 Upright Microscope with Dual Observation 1 unit

Order online anytime at www.polysciences.com

Polysciences, Inc.

400 Valley Road
Warrington, PA 18976

Polysciences Europe GmbH

Handelsstrasse 3
D-69214 Eppelheim
Germany

Asia-Pacific Headquarters

Polysciences Asia Pacific, Inc.
2F-1, 207 DunHua N. Rd.
Taipei, Taiwan 10595

[phone] / [phone]
[phone] / [phone] fax
[e-mail]
www.polysciences.com

(49) 6221-765767
(49) 6221-764620 fax
[e-mail]

(886) 2 8712 0600
(886) 2 8712 2677 fax
[e-mail]

Our catalog lists over 3,000 unique and specialty products that are used by histology professionals, lab technicians,
pathologists, chemists and scientists worldwide. Visit www.polysciences.com to learn more about our product lines.