IntelliPlex
TM
1000 πCode Processor
User Manual
PlexBio Co., Ltd.
PlexBio Co., Ltd.
6F-1, No. 351, Yangguang St., Neihu Dist.,
Taipei City 11491, Taiwan
Tel: +886-2-2627-5878．Fax: +886-2-2627-5979
http://www.plexbio.com
A04-016 V01 (RUO)
Standard Terms and Conditions for the Use of Products
Contract
The terms and conditions herein (hereinafter referred to as the “Standard Terms”) apply to all of
the products and services provided or will be provided by PlexBio Co., Ltd. (hereinafter referred
to as “PlexBio”). Any changes to the Standard Terms shall have no binding effect on PlexBio unless
the same has been agreed upon by an authorized representative of PlexBio in writing. The “Dealer”
referred to herein represents PlexBio (if the products are purchased from PlexBio directly), or the
distributor licensed by PlexBio. PlexBio expressly disagrees with any terms or conditions made by
the buyer in the purchase order or any of such which is different from or not included in this
Standard Terms.
The buyer’s acceptance, unpacking, or use of the products or services shall constitute his/her
acknowledgment and acceptance of the Standard Terms unconditionally. Once the buyer opens
the packaging of PlexBio products or uses the products or services in any manner, the buyer
acknowledges and accepts the Standard Terms unconditionally, and also agrees that the
Standard Terms constitute the contract which has binding effect on the buyer under law. Where
the buyer disagrees with the Standard Terms, he/she shall contact PlexBio immediately to
return the products before using the products or services in any manner, and he/she shall not
use the products or services in any manner. PlexBio has the right to amend the terms and
conditions regarding specification and service without notifying the buyer in advance if no
specification or service has been specified and identified in writing before.
Warranty
The warranty terms and conditions in this Standard Terms (the “Warranty”) apply to any
instruments, spare parts and services (collectively, the “Products”) purchased by the buyer from
PlexBio directly and situated in the territory of Taiwan. The Warranty excludes any activities other
than calibration, certification and maintenance. PlexBio will not provide any Warranty toward the
sale and use of the Products outside the territory of Taiwan. The Products distributed outside the
territory of Taiwan will be sold “As is”. PlexBio will provide the buyer with quality assurance
toward the spare parts purchased from PlexBio and that are used to maintain PlexBio instruments
under the same terms and conditions herein in any countries/territories other than Taiwan
around the world.
Particularly, the Warranty provided herein excludes any products, software or hardware provided
not by PlexBio. If the product is purchased from a PlexBio’s distributor, the Warranty shall be
provided by the distributor to the buyer in writing directly.
Quality Assurance
Products: Regardless of whether or not the buyer accepts said terms and conditions, for any
Products purchased from PlexBio directly, PlexBio will ensure that the performance of all the
Products meets the product specifications provided by PlexBio within 12 months from the
delivery date on (hereinafter referred to as the quality assurance period). PlexBio is entitled to
take one of the following actions against any defects found and reported during the quality
assurance period: (1) payment refund, or (2) defect repairs or spare parts replacements. The
expenses derived from said actions shall be borne by PlexBio.
Software: PlexBio ensures that all the installed software substantially meets the functions
described in the software documentation provided by PlexBio. Notwithstanding, PlexBio does
not guarantee that the software is error-free or may not be attacked by hackers or viruses. The
quality assurance period of the software is identical with that of the PlexBio product in which the
software is installed.
Service: PlexBio ensures that, where the buyer finds any inconformity of related services and
notifies PlexBio of the same in writing within 30 days after the related services are performed;
PlexBio shall be obligated to provide necessary services, assistance and consultation to correct
the inconformity.
General clauses: The Warranty and quality assurance provided are subject to the following
limitations, including (1) the said quality assurance does not apply to consumable materials,
accessories, normal wear and tear, damageable parts and fragile parts; (2) when the buyer asks
PlexBio to provide quality assurance work beyond normal working hours, PlexBio shall be entitled
to charge the buyer additional expenses; (3) the said quality assurance does not apply to the
following circumstances: accidents, modifications, unfair use, abuse, destruction, or disassembly
without authorization, or if the buyer fails to keep or operate in the manner as required, and(or)
do unauthorized maintenance, installation or service, and(or) incorporates or integrates any
product without permission from PlexBio into the PlexBio Products, or integrates the PlexBio
Products into the buyer’s environment or products, and(or) uses other software or user interface
provided by the buyer/vendor without PlexBio’s permission; (4) for the Products sold by PlexBio
but produced by another manufacturer, the quality assurance provided by PlexBio is only valid in
the residual period in which the manufacturer provides its quality assurance; (5) once any
Products is maintained and repaired by PlexBio, the buyer acknowledges that the maintenance
work will not extend the quality assurance period or derive any new quality assurance period.
Other than the Warranties and quality assurance explicitly provided in writing in this Standard
Terms, PlexBio does not provide any other warranties, including but not limited to the warranty
toward fitness for a specific purpose, explicitly or implicitly. If the dealer determines, on the basis
of its own judgment, that the buyer has misused the Products or failed to follow the instructions
to use the Products, the warranty provided by the dealer toward the sale of the Products shall be
invalid.
PlexBio shall not be liable for any direct or incidental damage caused by the use of or failure to
use the Products, including but not limited to, the loss in the process of operation, shutdown,
loss of revenue or profit, loss of the buyer’s product or other products, in addition to the liability
to be borne by the buyer to the supplier, or by the supplier due to such loss, and the labor or
other expenses, damages or losses caused by such Products, including personal injury or loss of
property, unless the personal injury or loss of property is caused by the PlexBio’s willful conduct
or gross negligence.
Maintenance: This maintenance clause applies to Products provided by PlexBio within the
territory of Taiwan only. The maintenance includes correction, verification, and regular
maintenance works only. PlexBio does not provide any warranty to the sales outside of Taiwan.
The Products sold outside Taiwan shall be labeled “original manufactured” and provided “as-is”.
In regards of any spare parts placed outside Taiwan and used for the purpose of maintaining the
PlexBio Products, PlexBio shall provide the quality assurance hereunder.
For any Products situated outside the territories of Taiwan for which maintenance is required:
(i) The buyer shall notify PlexBio in writing of the issue of the Products immediately and also
provide the details about the issue verified.
(ii) The buyer shall contact PlexBio or the service maintenance engineers trained and qualified
by PlexBio to evaluate the problems and verify the issues, and might need to bear the related
expenses derived from the maintenance or transportation of the Products.
(iii) The buyer shall return the Products at issue to PlexBio or the distributor as per PlexBio’s
request. PlexBio might analyze the Products returned. Upon verifying that no defects exist,
PlexBio will send the Products back to the buyer and the buyer shall bear the related
expenses and freight. Notwithstanding, if PlexBio verifies that some issues exist, PlexBio will
bear the freight. The buyer is not entitled to return the Products without PlexBio’s prior
written consent.
Notice to User
The use of this instrumentation and its associated software is covered by one or more
pending US and foreign patents owned by PlexBio Co., Ltd. The purchase of this instrument
and software includes nontransferable rights to use the instrument and software to practice
the methods described therein. No general patent or other license of any kind other than
this specific right of use from purchase is granted. For further information on purchasing
licenses for other applications can be obtained from PlexBio Co., Ltd. 6F-1, No. 351,
Yangguang St., Neihu District, Taipei City 11491, Taiwan.
Trademarks
, ,DeXipherTM, IntelliPlexTM, DigiPlexTM, πCode
trademarks or registered trademarks of PlexBio Co., Ltd. All other product names and any
registered and unregistered trademarks mentioned are used for identification purposes only
and remain the exclusive property of their respective owners.
Copyright
© 2017 PlexBio Co., Ltd. All rights reserved.
TM
, PlexBioTM, are
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IntelliPlexTM 1000 Code User Manual - Jul-17
Table of Contents
Chapter 1. Instruction .......................................................................................................................... 1
Overview .......................................................................................................................................................... 1
Warnings .......................................................................................................................................................... 1
Symbols ............................................................................................................................................................ 2
Chapter 2. Safety and Regulatory Issues ............................................................................................ 3
Intended Use ................................................................................................................................................... 3
Regulatory Labels ............................................................................................................................................ 3
Testing and Certifications ....................................................................................................................................................... 4
Safety Precautions .................................................................................................................................................................... 4
Chapter 3. IntelliPlex 1000 πCode Processor Technical Overview ................................................... 6
Introduction ..................................................................................................................................................... 6
Main Characteristics ....................................................................................................................................... 7
Shaking and Incubation .......................................................................................................................................................... 7
Washing ......................................................................................................................................................................................... 7
Fluorescence Labeling ............................................................................................................................................................. 7
System Technical Specification ...................................................................................................................... 8
Chapter 4. Installation Procedure ....................................................................................................... 9
Inspect the Instrument ................................................................................................................................... 9
Instrument Unpacking ................................................................................................................................. 10
Unpack Procedures................................................................................................................................................................ 10
List of Accessories .................................................................................................................................................................. 13
Instrument Assembling ............................................................................................................................... 14
Removing the Shipping Brackets...................................................................................................................................... 14
Place the Solution Trays ....................................................................................................................................................... 16
Connecting the Components............................................................................................................................................. 17
Chapter 5. Operation Procedure ...................................................................................................... 18
Initial Startup ................................................................................................................................................ 18
Touchscreen Introduction ........................................................................................................................... 19
Predefined Assay Protocols......................................................................................................................... 20
RUN the Built-in Assay Kit ................................................................................................................................................... 20
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Create a Custom Program ........................................................................................................................... 24
Procedure Introduction ....................................................................................................................................................... 25
How to Create a Customized New Program ................................................................................................................ 29
How to Overwrite a Program ............................................................................................................................................ 32
Special Applications ..................................................................................................................................... 33
Silicone Pad Assembly (Optional) .................................................................................................................................... 33
SAPE Solution Supplement ................................................................................................................................................. 36
Chapter 6. System Setting ................................................................................................................. 37
Maintenance................................................................................................................................................. 38
Renew Tank ............................................................................................................................................................................... 39
Clean Manifold ........................................................................................................................................................................ 41
Machine Reset .............................................................................................................................................. 42
Rinse Time .................................................................................................................................................... 43
Fluid Path ...................................................................................................................................................... 44
System Update ............................................................................................................................................. 45
Assay Update ................................................................................................................................................ 45
Date/Time ..................................................................................................................................................... 46
Language ....................................................................................................................................................... 46
Service Mode ................................................................................................................................................ 47
Log Status ...................................................................................................................................................... 47
Shutdown ...................................................................................................................................................... 48
Chapter 7. Maintenance ................................................................................................................... 49
Recommended Maintenance Schedule ..................................................................................................... 49
As Needed Maintenance ............................................................................................................................. 50
Long-term Shutdown Situation ........................................................................................................................................ 50
Fuse Replacement .................................................................................................................................................................. 50
Chapter 8. Contact Information ....................................................................................................... 51
Customer Service and Sales ........................................................................................................................ 51
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IntelliPlexTM 1000 Code User Manual - Jul-17
Chapter 1. Instruction
Overview
Read this manual carefully before using IntelliPlex 1000 πCode Processor. It provides essential
information about the following aspects of IntelliPlex 1000 πCode Processor:
• Safety and Regulatory Issues
• Labeling
• Technical Information
• Installation
• Operation Procedures
• Setting
• Routine Maintenance
Warnings
When operated in a safe environment according to the instructions in this user
manual, there are no known hazards associated with the instrument. However,
the operator should be aware of certain situations that could result in serious
injury or possible damage of the instrument. The protection provided by the
instrument may be impaired if it is used in a manner not specified in this
instruction.
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Symbols
General cautions and
warnings
Caution: pinch point
Warning: Biohazard
Warning: Hot Surface
Manufacturer
Date of Manufacture
Batch code
Serial number
Waste Electrical and
Electronic Equipment
Consult instructions for
use
ETL Mark
Federal Communications
Commission (FCC) Mark
The following symbols may appear throughout this manual with safety warnings, identification,
conditions, instructions and regulatory agencies.
Table 1. List of symbols
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Chapter 2. Safety and Regulatory Issues
Please read the safety information in this chapter before using IntelliPlex 1000 πCode Processor.
The system contains electrical and mechanical components which may result in injury and damage
of the instrument if handled improperly. Do not perform procedures on IntelliPlex 1000 πCode
Processor that are not specifically described in the user manual.
Intended Use
IntelliPlex 1000 πCode Processor is a unique, user-friendly 4-in-1 workstation and is intended to
use for 96-well plate washing, shaking, incubation and automated fluorescence labeling. The
workstation is specifically designed for πCodeTM MicroDisc during the hybridization, washing and
labeling steps required before fluorescent detection by PlexBioTM 100 Fluorescent Analyzer to get
optimized results. Additionally, this workstation can also be applied for ELISA washing only.
Regulatory Labels
The following label appears on the IntelliPlex 1000 πCode Processor. It displays the model, serial
number, lot number, power requirements, electrical safety certification and information of
manufacturer.
Figure 1. Label of IntelliPlex 1000 πCode Processor
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The following symbols may appear as necessary on the instrument and user manual which
Electrical
Cautions
General
Cautions
indicate the information notes and warnings.
Testing and Certifications
IntelliPlex 1000 πCode Processor has been tested and complies with the safety requirements. The
following agency mark will be on the label.
(ETL mark) (FCC mark)
Safety Precautions
In any situation in which user may encounter following symbols, please read and understand all
the safety precaution notes before the system operation.
This message is used to provide general caution information.
Please handle with extreme care when moving or relocating system as mishandling may
damage the system.
Lift the unit from the base only. DO NOT lift by or put excessive force on the plastic case.
DO NOT put excessive force on the stage or it may become tilted or bent. This will severely
interfere with the performance of the system.
This message is provided to avoid electric shock or possible damage
to the system.
DO NOT open or remove the covers on the system peripherals.
Use only power cords supplied with the system or cords with grounded outlets properly rated
for the system.
Ensure the power cord is connected to properly grounded AC outlets only.
Users should not perform any maintenance or cleaning of the electrical components in the
system.
If any malfunction happens, please turn off the power button and then disconnect the power
cable. The main plug is used as main disconnection device, not to position the equipment so
that it is difficult to unplug the mains plug.
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Waste Electrical
And Electronic
Equipment
(WEEE)
Pinch
Point
Hot Surface
The IntelliPlex 1000 πCode Processor has modules that move during the operation. The risk
of pinching hazards are presents. Please keep hands away from the moving part and do not
put your hands under the moving part.
Please do not touch the heating thermos plate surface until it has cooled.
Biohazard
Human and animal samples in the 96-well plate may contain biohazardous, infectious
materials. Please use personal protective equipment (PPE) including gloves and laboratory
coats. Please handle with extreme care when loading the plate onto the stage to prevent
spilling.
Within the European Union, the Waste Electrical and Electronic Equipment Directive
2002/96/EC requires the properly dispose of electrical and electronic equipment when it
reaches the end of its lifecycle.
If you are disposing of a IntelliPlex 1000 πCode Processor, please contact PlexBio Technical Support
for a Return Material Authorization (RMA) number at +886-2-2627-5878.
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IntelliPlexTM 1000 Code User Manual - Jul-17
Chapter 3. IntelliPlex 1000 πCode Processor
Technical Overview
This chapter describes the technology introduction, module characteristics and technical
specifications of IntelliPlex 1000 πCode Processor.
Introduction
IntelliPlex 1000 πCode Processor is the workstation specifically designed for πCode MicroDisc
during the hybridization, washing and labeling steps required before fluorescent detection by
PlexBio 100 Fluorescent Analyzer which minimizes user’s hands-on time. Users could also create
their own custom protocols from hybridization to fluorescence labeling by adjusting the related
parameters.
Features Include:
Easy to use: User-friendly interface with imbedded processing modes for PlexBio assays or
customization.
Multifunctional: Combines aspiration, buffer dispensing, shaking, rinsing, heating, and the
magnetic force application to minimize πCode MicroDiscs loss during washing steps.
Applicability: Designed for both molecular and immunoassays.
Touchscreen: Includes a 7-inch industrial touch panel.
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Main Characteristics
The multifunctions of IntelliPlex 1000 πCode Processor are including shaking , incubation, washing
and automated fluorescence labeling optimized for πCode MicroDisc. The characteristics are
provided as follows:
Shaking and Incubation
This function is designed for shaking standard 96-well microplates in thermostatic mode.
Features:
Easy to control time and temperature for operation
Simultaneous display of set and actual time, and temperature
Heating platform for standard 96-well microplates
Built-in PlexBio assay programs with no further setting needed
Washing
This function is designed to reduce hands-on time and human error during the πCode MicroDiscs
washing process.
Features:
Combines aspiration, buffer dispensing, shaking, automatic rinsing, and a magnetic plate for
minimizing πCode MicroDiscs loss during washing steps
Simultaneous display of the current washing cycles and status
Convenience: easy for operation and maintenance
Fluorescence Labeling
This function is designed to reduce hands-on time for the fluorescence labeling process. IntelliPlex
1000 πCode Processor can work with all types of Cy3 fluorescence readers. Use of SA-PE for πCode
MicroDiscs fluorescence labeling is most recommended.
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System Technical Specification
Model
IW100
PERFORMANCE
Processing time
(full plate)
3~6.5 min (one time wash)
Shaking
Timing setting range: 1min~23 hr59 min59 sec
Max. rate: 1200rpm
Incubator
Temperature range from RT+5℃ to 60℃
Safety
Plate position sensor and door sensor
Temperature accuracy
± 0.5°C
Temperature uniformity
1°C
Shaking accuracy
≤ 10%
Dispensing accuracy
≤ ± 6%, Measured under following conditions:
8-way manifold, 150 μL, PlexBio Wash Buffer (PBST)
Dispensing uniformity
≤ 4% CV, Measured under following conditions:
8-way manifold, 150 μL, PlexBio Wash Buffer (PBST)
MicroDisc loss
Around ≥ 75% of microdiscs retained after whole processes,
Measured under following condition:
500~1500 discs/well in 150μL PlexBio Wash Buffer
Residual volume
≤ 5μl /well, Measured under following conditions:
8-way manifold, 150 μL, PlexBio Wash Buffer
OPERATION CONDITIONS
Temperature/ Humidity
18℃~32℃/ 20~80%RH, non-condensing
Altitude
Up to 2000 meters (6561 ft) above mean sea level
Others
Reliability tests
STORAGE CONDITIONS
Temperature/ Humidity
-10℃~70 ℃/ 10~80% RH non-condensing
Others
Reliability tests
PHYSICAL CHARACTERISTICS
Power
100-240V, 50-60Hz, 250W
Dimensions
Without Bottle Carrier: (W)540mm*(D)408mm*(H)456mm
With Bottle Carrier: (W)540mm*(D)554mm*(H)456mm
Weight
38 kg
Fuse rating
T 5A H 250V
OTHERS
Pollution degree
2
Method of disposal
Electronic waste
Table 2. System Technical Specification
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Chapter 4. Installation Procedure
Cautions
Fig 2. The inside package of IntelliPlex 1000 πCode Processor
This chapter provides the detailed installation procedures. Note that IntelliPlex 1000 πCode
Processor should be installed and serviced by PlexBio’s qualified service engineers only. Before
setting up and unpacking the processor, please make sure the operation environmental
requirements:
Environmental Conditions
Indoor use only
Operating temperature: 18°C to 32°C
Operating relative humidity: 20% to 80%, non-condensing
Operating altitude: up to 2000m (6561 ft) above mean sea level
Storage temperature: -10°C to 70°C
Inspect the Instrument
IntelliPlex 1000 πCode Processor arrives in a large, corrugated cardboard overpack on skids.
Inspect the shipping box, packaging, instrument and accessories for signs of damage.
The package of IntelliPlex 1000 πCode Processor may be too heavy to
lift. Please lift the instrument from the bottom and do not pull, push,
or bend the case from the side.
IntelliPlex 1000 πCode Processor needs an operation area of 0.36
square meters. The system also needs to be placed at least 10
centimeters from the wall for components connection and for
efficient cooling.
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Instrument Unpacking
The following information provides the assembling process of the instrument.
Unpack Procedures
To set up IntelliPlex 1000 πCode Processor (Fig. 3 to Fig. 8):
1. Remove the carton buckle from the package box.
2. Remove the boxes containing IntelliPlex 1000 πCode Processor. The instrument is inside a
plastic bag and surrounded by foam inserts attached to a corrugated cardboard insert.
3. Remove the surrounded foam inserts and plastic bag.
4. Place the instrument on a stable and flat bench.
5. Remove two black cushions affixed on the frame of the front door.
Fig 3. Remove the carton buckle from the package box
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Fig 4. Remove the upper carton from its package
Fig 5. Remove accessories first then remove the foam inserts from its package
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IntelliPlexTM 1000 Code User Manual - Jul-17
Fig 6. Pulling out IntelliPlex 1000 πCode Processor from its package
Fig 7. The unpacked IntelliPlex 1000 πCode Processor
Fig 8. Remove two black cushions on the front door frame
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List of Accessories
Tubes*3
Manual*1
Accessory
box *1
Waste
bottle*1
Bottle
holder*1
Buffer
bottle*2
U tray*10
V tray*10
AC fuse*2
Silicon
pad*3
Power
cord*1
Silicon
pad
fixture*1
Before proceeding with the installation, please check the accessories provided with the
accessories list.
Table 3.1 Accessories in Top EPE
Table 3.2 Accessories in the box
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Instrument Assembling
Cautions
Removing the Shipping Brackets
Users must remove the shipping brackets before connecting the
components and before operating the moving manifold.
To remove the shipping brackets, please follow the steps 1 to 6.
Step1. Open the left side access door by loosening the screws on it (Fig.9).
Fig 9. Open the left side access door
Step 2. Remove the four screws (two small ones and two large ones) of the bracket A.
Step 3. Remove the five screws of the bracket B (Fig.10).
Fig 10. Remove the screws of bracket A and B
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IntelliPlexTM 1000 Code User Manual - Jul-17
Step 4. Remove the two screws of bracket C (Fig. 11).
Cautions
Fig 11. Remove the screws of pipette bracket
Step 5. Check all the removed brackets and screws.
Please be careful not to lose and save all removed brackets and
screws for future re-packaging and transporting.
Fig 12. All removed brackets
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Place the Solution Trays
Once complete removing the shipping brackets, please place the U-Tray and V-Tray in the
dedicated proper positions before connecting the components (Fig.13) and put the left side
access door back.
Fig 13. The positions of solution trays
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Connecting the Components
To connect the components:
1. Take out all the accessories including bottles, tubes and the AC power cord.
2. Plug the power cord into the back of the instrument.
3. Ensure that the on/off switch in the rear panel of the instrument is in the off position.
4. Fill up the wash buffer bottle with wash buffer and fill up the ddH2O bottle with distilled
water.
5. Connect the buffer tubes (Wash buffer and Distilled water) to the inlet connectors on the rear
panel of the instrument and to the corresponding bottle as well. See the Fig 14 for the
connected diagrams.
Fig 14. Connected diagrams
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Chapter 5. Operation Procedure
Before operation, please connect the power cord and the corresponding bottles and tubes. Make
sure the dedicated U Tray tank for washing buffer and V Tray tank for SAPE solution are placed
accordingly. Please make sure the bottles contain enough water and enough wash buffer.
Initial Startup
After setting up preparation, turn on the power at the backside of the instrument. The system will
run a system self-test every time users turn on the power. Please make sure buffer bottles are
filled with corresponding buffer and the waste bottle is empty. Click on the OK to complete system
self-test process.
Fig 15. Interface of system self-test
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Touchscreen Introduction
1.
2.
3.
4.
There are 4 main portions on the user interface homepage: (1) Main functions; (2) Menu; (3)
Date/Time; (4) Software version.
(1) There are 4 main function buttons for users to run the operation.
- Built-in PlexBio Molecular Assay
- Built-in PlexBio Immunoassay
- Built-in PlexBio Assays in Applied Science
- Custom Program
(2) Menu is for users to run the settings and maintenance procedures.
(3) Current date and time display.
(4) General information of certifications and software versions.
Fig 16. Homepage of the user interface
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Predefined Assay Protocols
IntelliPlex 1000 πCode Processor is installed with the predefined assay protocols from PlexBio’s
assay kits. There is no need to reset the protocol conditions each time before assay starts.
RUN the Built-in Assay Kit
For running the PlexBio assay kit, please see the Fig 17 to 22 for step 1 to 6.
Step 1. Select the built-in Plexbio assay types (Molecular Assay, Immunoassay or Applied
Science).
Step 2. Select the desired rows on the interface for processing and click on the Next ( ).
Step 3. Select the corresponding assay to operate.
Step 4. Confirm the shown procedure conditions are correct and click on the button START.
Step 5. Make sure the pop-up window notices are followed and click on the START to run the
assay.
Step 6. The interface will show the real-time status on the touchscreen until the assay is
finished.
Fig 17. Select the assay types
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Fig 18. Select the desired rows for processing
Fig 19. Select the corresponding assay
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Fig 20. Procedures confirmation
Fig 21. Pop-up window confirmation
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Fig 22. Real-time display of the assay operation status
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Create a Custom Program
This section describes how users can create and edit assay procedures based on their needs. See
the following information for the editable parameters.
Fig 23. Interface of custom program
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Procedure Introduction
Incubation: Users can edit the desired incubation temperature and time on this program. The
parameters for setting are listed as below.
(A) Heating temp.: Set up the desired heating temperature by sliding the setting bar or directly
key in the number into the screen square frame. Please note the temperature setting range
are limited from RT+5°C to 60°C .
(B) Heating period: Set up the desired heating period by directly key in the number into the
screen square frame by the order of Hour (H): Minute (M): Second (S). Please note the
temperature setting range are limited from 1min to 23hr59 min59 sec.
(C) Close lid before heating: The function is designed for users to incubate the plate in the
confined incubation space for the thermostatic environment. Please click to fill the square
if necessary. Note that the function should be used with silicone pad.
(D) Shake before reaching temp.: If the function is clicked to open, the plate will be shaken for
seconds to be mixed evenly once reaching the set temperature.
Fig 24. Parameters of custom incubation procedure
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Washing: Users can setup various conditions listed below during the washing step (Fig. 25).
(A) Washing Cycles: Setup the washing cycles for maximum 4 cycles.
(B) Washing Dispensing Volume: Users can either setup the 150 uL wash buffer dispensed for
the πCode system wash or use ELISA wash function for 200 uL washing.
(C) Last Dispense Volume: This is designed for users who want to dispense the buffer of 50 uL
into wells as the final step for dispersing πCode before decoding. It is usually used before
decoding analysis, but no need for ELISA assays.
(D) Magnetic Function: Users can choose to turn on the magnetic function to separate πCode
Microdiscs to peripheral bottom of the well from supernatant. The magnetic function is no
need for the ELISA assays.
(E) Clean Tip: When dealing with different samples in one 96-well microplate, to prevent any
carryover contamination between each sample placed in different rows, Clean Tip can do
the tip cleaning every time right before the next row approach. The tip cleaning will only be
performed in the 1st cycle during the washing procedure.
Fig 25. Parameters of custom washing procedure
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SAPE Reaction: This procedure is used for the fluorescence labeling step with automated SAPE
dispensing. The dispensed SAPE is fixed with 50uL per single well at 37°C
incubation for the desired incubation time and continues with the 4 washing
cycles. The heating period could be adjusted from 1min to 23hr59min59sec.
Fig 26. Parameters of custom SAPE procedure
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IntelliPlexTM 1000 Code User Manual - Jul-17
Disperse: This function is used to minimize the πCode MicroDiscs aggregates before decoding.
Pause: Pause is used to pause the process in progress for checking well status. The system will
be stopped during the pause and a warning window will be popped up, once users want
to restart the process, click to confirm restarting the process.
Fig 27. Disperse and Pause Procedure
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How to Create a Customized New Program
Users can create a program to perform the customized assay procedures sequentially in one run.
Please see the Fig 28 to 32 for step 1 to 7.
Step 1. Click on the Custom Program on the homepage.
Step 2. Select the desired rows on the interface for the processing and click on the Next.
Step 3. Select one program for editing from Custom 1 to 6.
Step 4. Click on ADD PROCESS, and choose the desired procedure from the drop-down menu
on the right side.
Step 5. Set detailed parameters for each procedure like temperature, time, washing cycles, etc,
and save the procedure.
Step 6. Repeat Step 4 and 5 to combine all procedures in order as the custom program steps.
Step 7. Confirm all the procedures and order are correct and then click on to save the
program and run the custom assay process.
NOTE:
1. Force touch the process on the touchscreen, users could delete the specific process with the
confirmation by the pop-up notice window.
2. Before operating processes, please make sure wash buffer and SAPE solution are supplemented
to the dedicated containers.
3. During the process operation, the touchscreen will real-time display the current status.
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Fig 28. Custom Program Homepage and Row Selection
Fig 29. Custom Program 1 to 6
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Fig 30. Select desired procedures to set up a process
Fig 31. Set detailed parameters for each process
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Custom Program1
Fig 32. Custom Program Process and Real-time Display
How to Overwrite a Program
IntelliPlex 1000 πCode Processor has the default for 6 programs for users to save.
Step 1. Select the Custom Program on the homepage.
Step 2. Select the desired rows on the interface for the processing and click on to Next step.
Step 3. Select one program for editing from Custom 1 to 6 to overwrite the program.
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Special Applications
Yellow Circle: Anchor
points for silicone pad
Red Triangle: Anchor
points for thermal lid
Yellow Circle: Anchor
points for silicone pad
Red Triangle: Anchor
points for thermal lid
This section describes the applications of necessary accessories before or during the assay
procedures.
Silicone Pad Assembly (Optional)
If users concern with the designed assay conditions such as contamination possibilities and
heating environments, the usage of silicone pad could be optionally applied.
During the incubation procedures of IntelliPlex 1000 πCode Processor, the microplate sealing tape
is replaced by silicone pad (Fig 33). The holding fixture (Fig 34) is used as a tool to assist users to
connect the silicone pad and reduce the contamination possibilities.
Fig 33. Silicone Pad
Fig 34. Holding Fixture
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Please follow the operation steps listed below to connect the silicone pad:
Step 1. Take out one silicone pad without touching the inner side (bump on the inner side)
which will cover on the 96-well plate directly.
Step 2. Let the inner side of silicone pad face to the holding fixture and connect the four anchor
points (Fig 35) together. Make sure the silicone pad is snap-fit with the holding fixture
(Fig 36).
Fig 35. Direction of assembling silicone pad to the holding fixture
Fig 36. Assembled silicone pad on the holding fixture
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IntelliPlexTM 1000 Code User Manual - Jul-17
Step 3. Let the holding fixture, which assembled with silicone pad, face to thermal lid and
connect another four anchor points (Fig 37) together.
Fig 37. Connect silicone pad via holding fixture to the thermal lid
Step 4. Remove the holding fixture and make sure the silicone pad is snap-fit to the thermal lid
(Fig 38).
Fig 38. Remove holding fixture and silicone pad is connected
Note: It is not recommended to reuse the silicone pad under different assay procedures though it
could be cleaned with 70% alcohol and tolerated the temperature for up to 200℃.
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SAPE Solution Supplement
The SAPE solution supplement is necessary for the built-in PlexBio assays or the customized assays
which need SAPE reaction process. The SAPE solution tank (V Tray) position is shown as Fig. 39.
The tank can be filled with SAPE solution directly followed by the desired SAPE solution volume
calculation. Please note that the dead volume of the SAPE solution of IntelliPlex 1000 πCode
Processor is 500 µL and the minimum usage of SA-PE is one row. Normally, the needed SAPE
solution for assay reaction per well is 50 µL.
Calculation Example:
For 3 rows reaction, the SA-PE solution needed is
400 µL x 3 rows + 500 µL = 1.7mL (at least).
In order to ensure the instrument has sufficient solution to dispense, pipet extra volume into the
solution tank is recommended.
Note: It is suggested to supplement the SAPE solution by pipetting and reuse the SAPE solution
leftover are not recommended.
Fig 39. The positions of two types of tanks (modules)
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Chapter 6. System Setting
This section describes the tool menu for users to do the settings. Each setting function (Fig 40)
will be described in details as follows.
Fig 40. The menu setting list
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Maintenance
This section describes the maintenance of the buffer tank renew and manifold cleaning (Fig 41).
Fig 41. Maintenance from tool menu
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Renew Tank
Wash buffer (U Tray)
SAPE solution (V Tray)
Periodically renewing the wash buffer and SAPE solution tanks helps to keep the tanks clean and
reduce the possibility to have the salts crystals and contamination. Users could renew the tanks
manually. There are two types of tanks including the wash buffer tank (U Tray) and the SAPE
solution tank (V Tray), please see Fig 42.
Fig 42. The position of two types of tanks (modules)
There is no need to take out the fixed white modules from the instrument to renew the wash
buffer and SAPE solution tanks, the transparent tanks can be taken out from the fixed modules
directly and manually.
For the wash buffer tank (U Tray) renew, please follow the steps described below:
Step 1. Select the Setting on the menu page.
Step 2. Click on the Maintenance button.
Step 3. Choose the Renew Tank and the manifold will move backwards to reveal the tanks for
users to replace manually (Fig 43).
Note: It is recommended to renew wash buffer tank (U Tray) once a week to reduce the possibility
of contamination or the crystal clogging.
For the SAPE solution tank (V Tray) renew, users could renew the tank directly without any setting
procedures. It is recommended to renew SAPE solution tank (V Tray) every time prior to use.
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IntelliPlexTM 1000 Code User Manual - Jul-17
Fig 43. Renew wash buffer tank
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Clean Manifold
Regular rinsing helps to keep the manifold clean and reduce the possibility to have the salts
crystals. If the crystals block the manifold tips or any visible dust on the manifold tips, users could
clean the manifold manually by using the fine needles or thin iron wire. Please follow the steps
described below (Fig 44) to do the clean manifold:
Step 1. Select the Menu on the homepage.
Step 2. Click on the Maintenance button.
Step 3. Choose the Clean Manifold and the dispensing and sucking module will move forward
to reveal the manifold for users to clean manually.
Note: It is not recommended to disassemble the manifold tips by users. If there is any crystals or
dust on manifold tips which cannot cleaning away, please contact PlexBio Co., Ltd. or local
distributors for service.
Fig 44. Clean the manifold
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Machine Reset
Users can use this function to return the manifold, the thermal lid, and the magnetic plate back
to their original setting positions.
Step 1. Select the Setting on the menu.
Step 2. Choose the Machine Reset to reset the all modules
Fig 45. IntelliPlex 1000 machine reset
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Rinse Time
The rinse function is used to keep pumps and tubes wet to prevent clogging during the instrument
processing. Otherwise, the pump may fail due to the salt crystals formation from wash buffer.
Hence, users can setup the rinse time for system to do rinsing automatically.
Step 1. Select the Rinse Time on the setting menu.
Step 2. Set up the Rinse Time. If it is set as 2hr, the system will do the auto-rinse when it has
been stopped running for 2 hours.
Note: After the auto-rinse procedure, users could operate the prime step by confirming the pop-
up window, if necessary.
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Fluid Path
This function is used to rinse or prime the fluid path after the system experiences an abnormal
stop such as a crash. When an abnormal stop happens and the system reboots again, some
contamination from the fluid path may affect following procedures. It is crucial for priming or
rinsing the fluid path first. There are three options in this function including RINSE (ddH2O), PRIME
(wash buffer) and RINSE & PRIME.
Step 1. Select the Menu on the homepage.
Step 2. Select the Fluid Path on the tool menu.
Step 3. Choose RINSE, PRIME or RINSE/PRIME.
Step 4. Press OK to start running.
Fig 46. Fluid path process
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System Update
Upgrade the software in the instrument for the new versions through USB drive. If you need more
information, please contact PlexBio Co., Ltd.
Assay Update
Upgrade the assay software to renew the built-in assay protocols through USB drive. If you need
more information, please contact PlexBio Co., Ltd.
Fig 47. Update Assay Protocols
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Date/Time
Setup the current Date and Time for the instruments.
Fig 48. Date and time setting
Language
Set up the language of the user interface for English or Simplified Chinese.
Fig 49. Language setting
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IntelliPlexTM 1000 Code User Manual - Jul-17
Service Mode
The Service Mode is mainly for the factory to do the instrument service. Do not access the service
mode without any instructions.
Fig 50. Service Mode
Log Status
Users could check the system log history through the Status on the homepage.
Step 1. Select the Status on the menu.
Step 2. Choose the date to check the detailed log.
Step 3. Choose the procedure to check the detailed log.
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Shutdown
After the operation of system, users should run the program of Shutdown every time to do the
pump and tubes rinsing followed by the instrument close. Users should not turn off the power on
the instrument backside directly in case of salt crystals formation without rinsing.
Step 1. Select the Shutdown on the menu.
Step 2. Press OK to run the auto-rinse followed by the system turn-off.
Step 3. After the system is off, switch off the power button and unplug the power cord to
totally disconnect the instrument.
Fig 51. System shutdown program
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Chapter 7. Maintenance
Action
Daily
Weekly
Monthly
Yearly
Check/ Empty waste bottles
✔
Clean bottles
✔
Clean external surface
✔
Clean fluid path tubes with 70% alcohol
✔
Lubricate the stage guideway to prevent
stage positioning error or motor failure.
✔
Replace the plastic tips
✔
Replace the fluid path tubes
✔
Re-position all axes of moving modules
✔
General
Cautions
Biohazard
Please observe the following general maintenance precautions:
Personnel that use, maintain, or clean IntelliPlex 1000 πCode Processor should be trained in
standard laboratory safety practices and should follow those practices when handling the
instrument.
Samples and waste fluid may contain biohazardous material. Where
exposure to biohazardous material exists, follow appropriate biosafety procedures, use personal protective equipment, and use
ventilation devices.
Users should not perform any maintenance or cleaning of the
electrical components in the system without training.
Recommended Maintenance Schedule
In spite of the IntelliPlex 1000 πCode Processor has been systematically tested and calibrated
before shipment, preventative actions are required to maintain the properly functions. The
recommended maintenance schedule should be checked and calibrated periodically by a qualified
service technician. See the specified section of the instruction manual for further details.
For more information on Preventive Maintenance or Extended Warranties, please contact
Technical Services Department of PlexBio Co., Ltd. or qualified local distributors.
Important: The affecting factors associated with users’ assays may require performing some or all
of the procedures more frequently than recommended in the schedule.
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IntelliPlexTM 1000 Code User Manual - Jul-17
As Needed Maintenance
Long-term Shutdown Situation
If the IntelliPlex 1000 πCode Processor has been known not to operate for long time, it is
recommended to clean the fluid path, as procedures listed as follows, to reduce the possibility of
salt crystals formation in the fluid path.
Step 1. Use another buffer bottle contained 70% alcohol to replace the ddH
connect the ddH2O tube.
Step 2. Run the Rinse of Fluid Path on the setting menu for four times.
Step 3. Connect the 70% alcohol buffer to the wash buffer tube.
Step 4. Run the Prime of Fluid Path on the setting menu for four times.
Step 5. Remove all the buffer bottles to disconnect with tubes.
Step 6. Run the Rinse/Prime of Fluid Path on the setting menu for three times to empty the
tubes with volatile of alcohol.
Step 7. Run the Shutdown to turn off the instrument.
O bottle and
2
Fuse Replacement
If the fuse in the instrument is broken, please follow the next steps to replace it.
Step 1. Pull out the fuse holder from the power socket.
Step 2. Take out the broken fuse and replace it with a new one from the accessory box.
Step 3. Push the fuse holder back to its original place.
Fig52. A Fuse holder Fig53. B Fuse in the fuse holder
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IntelliPlexTM 1000 Code User Manual - Jul-17
Chapter 8. Contact Information
PlexBio Co., Ltd.
6F-1, No. 351, Yangguang St., Neihu Dist.,
Taipei City 11491, Taiwan
Customer Service and Sales
Web: http://www.plexbio.com
Phone: +886-2-2627-5878
Fax: +886-2-2627-5979
Order info: order@plexbio.com
General info: marketing@plexbio.com
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