Phenix PH50-2A42H-A, PH50-1A32H-A, PH50-1A31R-A, PH50-1A42L-A, PH50-2A43L-A User Manual

Phenix PH50 monoculaire microscoop

4503000

 Although the design of our product provided the best safety for your usage.

But, inaccuracy of operation method or neglect the usage elucidation probably
cause body hurt and the prop-erty lose. For your safety. Before using this
instrument ,please carefulness reading the instructions.

 Don’t lose the instruction manual, please saving it at near the instrument, in

order to consult at any time.

 In this instructions, the safety hint is expressed the follow sign. Please you

must obey the sign, insure the right safety operation.

Contents

1. Main Features .................................................................................................................................. 5

2. Usage and Coverage Area ............................................................................................................... 5

3. Electrical Capability ......................................................................................................................... 5

4. Working Circumstance of Microscope ............................................................................................ 6

5. Structure and Nomenclature ........................................................................................................... 7

6. Instrument’s Installation ................................................................................................................. 8

7. Operation Procedure ..................................................................................................................... 10

8. Digital Part, Installation and Useage of Parts ................................................................................ 16

9. Care and maintenance .................................................................................................................. 17

10. Replacing light bulb and fuse .................................................................................................... 18

11. Troubleshooting and Handling .................................................................................................. 20

12. Configuration table .................................................................................................................... 21

1. Main Features

PH50 series biological microscope have Mono Viewing and Bino Viewing for
choice. And can be built-in CMOS or CCD camera system equipped with USB
output ports, making use of computer image processing software and computer
multimedia technology, so that you have observed show for variety of video
figures , can capture images of static and dynamic video, with Objective
measurement and a variety of image processing functions.

2. Usage and Coverage Area

PH50 is an ideal instrument for use in clinic and hospital laboratory, research
institution, university in the research of biology, pathology, bacteriology, also for
educational laboratory sessions.

3. Electrical Capability

3.1 Using the specified power supply:

 For Domestic market: input Voltage: AC. 220V 50Hz.
 For overseas market: input voltage: AC. 110V 60Hz ～ AC. 230V 50Hz

3.2 Using Specified Light bulb, Power wire, and Fuse

 Halogen light: 12V/20W Fuse: 0.5A
 Power wire: with earthed wire

4. Working Circumstance of Microscope

This microscope is a precise optical instrument, if the usage or safekeeping isn’t
appropriate, it will cause the instrument damaged or influence precision. While
choose to use the place, please consider the following condition

 The place for this microscope should not be too bright, and direct sun shine on

the instrument should be avoided.

 The working temperature: 0℃-- 40℃. The maximum relative humidity: 85%,

the heat and humidity can stimulate the growth of mildew, which will cause the
damage to Microscopes and shorten its using life.

 Dust can deteriorate the optical performance, avoid to use under such

circumstance.

 Using the microscope On the steady and aclinic workbench.

5. Structure and Nomenclature

Fig.1

(1) Viewing head：Viewing components.
(2) Stand: The microscope’s main body.
(3) Revolving nosepiece：Used to select objectives needed(Locate with sound).
(4) Objective：Magnify specimen for the first time.
(5) Slide clip：Clip specimen.
(6) Mechanical stage：Lay specimen
(7) Condenser moving knob：Adjust the height of condenser.
(8) Power plug：Connect power supply.
(9) Fine focusing knob：Tiny focusing.
(10) Coarse focusing knob：Large focusing.
(11) Power switch：Overall power switch of an instrument.
(12) Fuse：fixup fuse.

(13) Elasticity accommodable ring.

(14) Eyepieces: Magnify specimen for the second time, put up observation.

(15) Condenser screw bolt: Fixup condenser.
(16) Axis Y moving knob：Adjust working stage transverse motion.
(17) Axis X moving knob：Adjust working stage lengthways motion.

6. Instrument’s Installation

6.1 Place the microscope on a flat and stable working table, while moving the

instrument, especially the optical parts, make sure to avoid to contact the

lenses surface with hand or artic with

grease.6.2 On the way to transport

carry the objective table, it's surface

will be covered with the protection film,

before use please tear up the prot­ected film（Fig.2）。

6.3 Place gently the viewing head into the Installation hole. Then use one hand to

press the viewing head from above while other hand fastens the screw located

at the both sides of view head.(Fig.3)

Fig.2

PROTECTION FILM

Fig.3

6.4 Turn on the power: after all above procedures, please insert the Power plug on

the wall outlet, and turn on the Power switch , make sure you are using the right

voltage.

6.5 Insert the Eyepiece: slide the eyepiece into the Eyepiece tube. (Fig.4)

Fig.4

7. Operation Procedure

7.1 Turn on the power

8.1.1 Turn the power switch(turn switch to "-" ), make the bulb give out light。

8.1.2 Then revolve the handwheel of brightness regulates to regulate the

brightness of filed .

7.2 Restoration to adjust diopter of tube

To adjust diopter of tube on the R/L eyepiece tube, Make its bottom edge with

engrave the line to align, use the same method, adjust the left diopter .

7.3 Adjusting pupil distance

By adjusting pupil distance and Diopter ring on eyepiece, to eliminate the

Parallex, and to get the sharp and comfortable viewing. While using bino head,

And field of view is two intersectant circles, by rotating the eyepiece tube, the Eye

Relief has been changed, the field of view became a round view completely

coincided. (Fig.5)

Fig.5

7.4 Mounting on the specimen

7.4.1 Turn apart the Specimen

Clips, put into the Specimen,

and use slide clip to hold it.
Release the fingers；make sure

that the viewing area right in the

middle of stage.(Fig.6)

7.4.2 Adjust the stage hand wheel

make sure that viewed field locates

objec -tive under. (Fig.7)

7.5 Using 10X objective focusing

7.5.1 By rotating the Revolving

nosepiece，make sure that lower

magnification objectives(4X or 10X)

are in the optical center, and get a

wide field of view.(Fig.8).

7.5.2 Rotating Coarse Focusing wheel，
moving the stage to the tiptop (Fig.9)。

7.5.3 Adjust Coarse focusing knob to find

sharp image. Adjust the diopter ring on

the diopter eyepiece until the image is

clear.(Fig.10)

7.6 Adjust fine focusing knob to find clear image. (Fig.11)

7.7 Rotating the Condenser moving knob, make condenser move highest and a little

lower. If you obverse dispersion image in view field , please adjust

condenser(Fig.13).

7.8 Rotating Revolving nosepiece，choose magnification that you need.

7.9 Adjust the Viewing head setscrew, in order to objective easy. (Fig.14)

7.10 If objective selected is 100X oil (spring), use oil as observing medium.

By this the quality of image can be improved and the specimen is observed

clear. (Fig.15)

7.10.1 Then move the specimen into the view filed and rotate the nosepiece to

view field.

7.10.2 Extrude a little oil from oil bottle. Dip oil onto the pot to observe.

7.10.3 Then move the specimen into the view filed and rotate the nosepiece to

view field. The interspace between top objective and coverglass must be full of
oil. Only this can the observation be done.。

7.11 When the microscope is not needed, turn off the power switch.

8. Digital Part, Installation and Useage of Parts

8.1 Take out the digital observation, Place the pin at locating hole of the observation,

Load the observation lightly, Press the upper observation softly by one hand ,

Fixing the screw on both sides of the body. At the time of installation to ensuret

digital head tight.

8.2 Put the end of USB cable into USB port of the back of the computer, the other

end into USB port of the back of equipment, software installation see "PHMIAS

family of software instruction manual" (Fig 16).

8.3 The installation of an external CCD (for TV observation or trinocular head) firstly

adjusting microscope to clear field of view, Connecting CCD base with CCD

moving into TV tube, "TV / Video" ( TV / AV) is set to "AV", as video display,

rotating adjustment ring (Fig 17), the video will be clear.

9. Care and maintenance

9.1 The power switch at the back side of Base is main power control. If the

microscope is no longer in use, please switch off the power to avoid the

electronic elements in working status. If the microscope will not be used for a

long period , please unplug the power wire from socket. Also keep various

accessories in safe places.

9.2 Using leaning gauze (or Silk, absorbent cotton )soaked with some ethanol to clean

the microscope body. After cleaning, cover it with dust cover.

9.3 Cleaning the Lenses：Use blower or soft cloth to wipe the surface dust. The

contaminated dust, finger prints can be wipe off by lens paper or soft clothe
soaked with blending (20-30% alcohol – 70-80% ether).

9.4 Cleaning the Microscope Surface：Clean with soft cloth, for severe stain, please

clean with neutral detergent.

9.5 Microscope Storage：If the microscope will not be used for quite a long period of

time, please turn off the power, cool down the light bulb, mask it with dust cover

and then put it back into packaging case. Store it in a cool, dry, clean place free

of Acid or Alkali steam. As this will cause mildew on the lenses.

9.6 Routine Inspection：In order to maintain the performance of

microscope, please conduct routine inspection and maintenance.

10. Replacing light bulb and fuse

10.1 Replacing light bulb

10.1.1 Turn off the microscope ,unplug the power cord from socket.

10.1.2 Waiting for 30min, until it cools down.

10.1.3 Screw off the light base, and pull out the light base board, and rotate at some

angel until the light base comes out. Take out the damaged bulb, and replace

with a new one. While inserting the new bulb, please make sure to get the

good contact and solid connection between light bulb and Base. While taking

bulb with hand, make sure to wear a glove to avoid the finger print being left

on the bulb. (Finger print will erode the bulb surface, lower the brightness,

shorten the bulb working life).(Fig.18)

10.2 Replace the fuse：Pull out the Fuse compartment cover, take out the damaged

fuse, and replace the new fuse and then build in on the Compartment cover.

11. Troubleshooting and Handling

Symptom

cause

Handling

Optical Parts

Uneven brightness
inside Field of view

Bad nosepiece
positioning（Different stalk of
light road）

Revolving the nosepiece, make it
into right position

Condenser position too low

Adjust the height of

Not centering with Condenser

Readjust the condenser

Wrong installation of light bulb

Check if the light bulb has been
mounted correctly

There are dirty vestige or dusts
on the specimen, condenser,
objective, eyepiece.

Wipe the relevant parts

Iris diaphragm opened too small

Make diaphragm larger

Dirty field of view

Dirty lens surface

Clean the surface

Slide cover dirty

Clean the surface

Resolution is not so

good Image is not

sharp

Slide has not been applied with slide cover

Apply with slide cover

Slide cover is too thin or too
thick

Use standard (thickness 0.17mm)
slide cover

Slide has been put on with the wrong side up.

Reverse it.

Oil on the dry objective

Wipe oil off

Oil objective without oil immersion

Immerse with oil

Dirty lenses surface (on eyepiece
and objective)

Clean the surface

There is a air bubble inside
immersed oil.

Remove the air bubble
Wrong immersion oil

Using standard oil

Iris diaphragm has been opened
too small

Enlarge the diaphragm

Condenser position too low

Adjust the height of

The single side of

picture is dark

Bad nosepiece positioning

Revolving the nosepiece, make it
into right position

The slide is higher mechanical
stage

The slide should be tight on the
stage

The image is the thin

yellow

The light bulb electric voltage is
lower

Revolve to adjust the light knob,
regulate the bright degree

Without using the blue filter

Using the blue filter

The image is ver y

bright

The light bulb electric voltage is
higher

Revolve to adjust the light knob,
regulate the bright degree

Don’t focusing at

using the higher

magnification

objectives.

Slide has been put on with the
wrong side up.

Reverse it.

Slide cover is too thick

Use standard (thickness 0.17mm)
slide cover

Objective is not hard up

Screw down objective

the left and right
image is notsuperpo­sition by the double
eyepiece viewing.

Without adjusting the Pupil
distance

Adjusting the Pupil distance

The Diopter adjuster is not good

Adjusting the Diopter

Light can not

turned on

Power was not turned on

Check if the power was switched on
,if the supplied voltage same with
specified voltage

Bad contact between power
socket and outlet

Check if the connection between
power cord and power

Light bulb have been melted

Replace light bulb

Fuse have been melted

Replace fuse

Light blink

Bad contact between light bulb
or bulb socket

Re-screw the bulb securely

Power_wire contact is bad

Check Power_wire
contact

12. Configuration table

PH50 Biological Microscope：Specification

PH50 Series

Product sequence number

1 2 3 4 5 6 7

Name

Specification

1A31
R-A

1A32
H-A

1A4
2L-A

1B4
3L-A

2A42
H-A

2A4
3L-A

3A4
3L-A

Total
Magnification

40X-640X

● ●

40X-1600X

● ● ● ●

●

Eyepiece

Wide Field WF10X/18mm

● ● ● ● ● ● ●

Wide Field WF16X/13mm

● ● ● ● ● ● ●

Lens Tube

Monocular，Inclined 45 degree，360
degrer Rotatable

● ● ●

Moncoular TV，Inclined 45
degree，360 degree Rotatable

●

Monocular，Inclined
45，360°Rotatable

Dual Observing，Inclined 45
degree，360 Rotating

genel-style binocular head，Inclined
30 degree，Interpupillary
Distance55－75mm

● ●

sliding-style binocular head，Inclined
45 degree，Interpupillary 55－75mm

Genel-style monocular head, Inclined
30 degree, Interpupillary Distance 55­75mm(w/push-and-pull rod）

●
Nosepiece
Triplicate

● ●

Quadruple

● ● ● ●

●

Achromatic
Objectives

4X，10X，40X（S）

● ● ● ● ● ● ●

100X（O,S）

● ● ● ●

●

Plan
Achromatic
Objectives

PL4X，PL10X，PL40X（S），
PL100X（O,S）

Focusing
System

Coarse and Fine with Different Axis
Coarse Adjustment:22mm, Fine
Adjustment:1.8m

Coarse and Fine Focusing Coaxal
Coarse Range:22mm, Fine Precision
Scale:0.004mm

● ● ● ● ● ● ●

Working
Stage

Monolayer Working Stage with Clips
Area:120mmX110mm

●

Monolayer Working Stage with
Sliding stage
Area:120mm×110mm
Distance：60×30mm

● ●

●

Double Layers with Mechanical
Sliding Stage
120mm×115mm，50mm×35mm

● ● ●

Condenser

Single lens，N.A=0.65
with Iris Diaphragm and Filter stand

●

Abbe Type，N.A=1.25
with Iris Diaphragm and Filter stand

● ●

●

Abbe Type，N.A=1.25
with Iris Diaphragm and handwheel
stand

● ● ●
Illumination

Critical Illumination

● ● ● ● ● ●

Lamp-house
Plan-concave mirror，φ50mm

●

Halogen
lamp，12V/20W，Brightness
Adjustable

LED 1W，rechargable, brightness
adjustable

● ●

● ●

LED 1W，non-rechargable,brightness
adjustable

Power

Onoff power，suitable for AC 96V­246V

Power adapter

● ● ● ● ● ●

Package
Carton box with inner foam

● ● ● ● ● ● ●

Aluminium Metal box

Others

TV viewing head with simple CCD
adapter

PH50 Digital Microscope

Specification for parts

PH50 Series

Name

Specifiaction

DM048U

DB048U

DB130U

DB200U

Main optical machine part

Total
Magnification

40X-1600X

● ● ●

●

Eyepiece

Wide field
eyepiece:WF10X/18mm

●

Wide field

eyepiece:WF16X/15mm

●

Wide field

eyepiece::F10X/18mm，diopter
adjustable

● ●

●

Wide field eyepiece:
WF16X/15mm
Diopter adjustable

● ●

●

Digital viewing
head
monocular，Inclined 45 degree
CMOS Camera Integrated

●

genel-style binocular head
Inclined 30 degree
Interpupillary distance:55-75mm
CMOS Camera Integrated

● ●

●

Nosepiece

Quadruple

● ● ●

●

Achromatic
Objectives

4X ● ● ●

10X ● ● ●

40X（S）

● ● ●

100X（S,O）

● ● ●

Plan Achromatic
Objectives

PL4X

●

PL10X

●

PL40X（S）

●

PL100X（S,O）

●

Stand
Focusing system

Coarse and Fine Focusing
Coaxal system:22mm，Minimal
Graduation：0.004mm

● ● ●

●

Working stage

Double layers with mechanical
sliding stage
140mm×132mm，50mm×30m
m

● ● ●

●

Condenser

Abbe type，N.A=1.25
with Iris Diaphragm and
handwheel stand

● ● ●

●

Lamp-house

LED 1W，rechargable,

● ● ●

●

brightness adjustable

Power

transformer

● ● ●

●

Package

Aluminium Metal box

● ● ●

●

Dividing ruler

0.01mm

● ● ●

●

Slides

Plant slides

● ● ●

●

CCD & CMOS

CCD Camera

470K Pixels，480 TV Line

CMOS Camera

480K Pixels，800×600

● ●

CMOS Camera

1300K Pixels，1280×1024

●

CMOS Camera

2000 Pixels，1600×1200

●

Image processing

AV-USB，720×576

Signal Output port
Video Output port

USB port

● ● ●

●

Signal Output Line
Video cable

USB signal cable

● ● ●

●

Micro Image Processing and Analyzing Software

Micro Image
Software

PHMIAS2006 Ver2.1(English
Edition)

● ● ●

●