PCRmax Ureaplasma parvum Instruction Manual

Urease complex component
UreC (ureC) gene

Ureaplasma parvum

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Ureaplasma parvum(formerly known asUreaplasma urealyticum biovar1) is a
Ureaplasma which are among the most common bacteria isolated from the human
urogenital tract.

Ureaplasmascan produce asymptomatic infections or disease characterized by an
exaggerated inflammatory response.

Ureaplasma parvumhas a circular chromosome consisting of 751,719 base pairs. The GC
content is 25% which is relatively low compared to other species.

The species prefers to grow in acidic pH between 6.0-6.5. It colonizes the human urinary
and genital tracts causing a variety of diseases that are discussed below.

Mutations within the 23Sr RNA and ribosomal proteins L4 and L22 have caused macrolide
resistance within Ureaplasma parvum. This has allowed it to survive in environments
normally hostile to other microbes such as the acidic urinary-genital tract and enabled it to
be resistant to antimicrobial drugs.

Ureaplasma parvumhas been categorized as a mucosal parasite living within the genito­urinary tracts. It is a mycoplasma and pathogenic ureolytic mollicute which can cause male
urethritis, supperative arthritis, adverse pregnancy outcomes, chorioamnionitis, surgical
wound infections, neonatal meningitis, pelvic inflammatory diseases, pyelonephritis, and
neonatal disease.

Ureaplasma parvumcan cause infertility via placental inflammation and infection of the
amniotic sac during early pregnancies. Infection of the lower lung can cause chronic lung
disease in premature babies and/or bronco-pulmonary dysplasia. Lower respiratory
infection can also cause infants with low birth weights leading to congenital pneumonia,
meningitis, and even death.

Treatment is via a prolonged dose of antibiotic such as Doxycycline.

Nucleic Acid Testing targeting the ureC gene is a reliable method of detecting U.parvum as
this is a consistent stable gene in an otherwise mutable pathogen.

Introduction to Ureaplasma parvum

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MIN

MAX

The PCR Max qPCR Kit for Ureaplasma parvum (U.parvum) genomes is designed for the
in vitro quantification of U.parvum genomes. The kit is designed to have the broadest
detection profile possible whilst remaining specific to the U.parvum genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
U.parvum sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• U.parvum specific primer/probe mix (150 reactions BROWN)

FAM labelled

• U.parvum positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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