PCRmax Tsukamurella inchonensis Instruction Manual
DNA gyrase B subunit (gyrB)
gene
Tsukamurella
inchonensis
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
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Tsukamurella species are aerobic gram-positive rod shaped bacteria from the order
Actinomycetales. It is an opportunistic pathogen and immunocomprimised individuals are
most often at risk. It has been isolated from the lungs of infected indivuals and has also
been identified as a cause of septicemia, in many cases due to infected hospital
equipment. Tsukamurella spp. exist primarily as environmental saprophytes in soil, water
and foam (thick stable scum on aeration vessels and sedimentation tanks) of activated
sludge.
Infections with Tsukamurella species have been associated with chronic lung diseases,
immune suppression (leukaemia, tumours, HIV/AIDS infection) and post-operative wound
infections. Tsukamurella were reported in four cases of catheter-related bacteraemia and
in individual cases including chronic lung infection, necrotizing tenosynovitis with
subcutaneous abscesses, cutaneous and bone infections, meningitis and peritonitis.
Introduction to Tsukamurella inchonensis
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MIN
MAX
The PCR Max qPCR Kit for Tsukamurella inchonensis (T.inchonensis) genomes is
designed for the in vitro quantification of T.inchonensis genomes. The kit is designed to
have the broadest detection profile possible whilst remaining specific to the T.inchonensis
genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
T.inchonensis sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
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Kit Contents
• T.inchonensis specific primer/probe mix (150 reactions BROWN)
FAM labelled
• T.inchonensis positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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