PCRmax Trypanosoma evansi Instruction Manual
variable surface glycoprotein
(VSG) gene
Trypanosoma evansi
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
1
Trypanosoma evansi is the causative agent of surra disease, one of the most widespread
trypanosomal diseases. The disease is endemic in many parts of Africa and Asia where
thousands of animals die during outbreaks each year. Horses and camels infected with the
disease represent a significant economic loss to the global industry. It is not known to be
zoonotic but there has been such a documented case in India. T. evansi can be identified
by its longitudinal, fusiform appearance, a flagellum that protrudes from the basal body
allowing it to swim forward, an undulating membrane and a kinetoplast, lying behind the
basal body.
T. evansi is transmitted mechanically by a number of arthropod vectors such as tabanids,
stable flies and tse tse flies. They can also be spread during nursing, copulation or
ingestion of infected tissues. Unlike other trypanosome species, T.evansi does not
undergo development in an intermediate host before reaching the adult state. In contrast, it
multiplies in a mammalian host by binary fission in the trypomastigote stage.
Animals with acute infection usually have a short clinical stage and die within weeks to
months of infection. In contrast, some chronic infections may persist for years. The
incubation period ranges from 5 to 50 days and the clinical signs may vary between
species. They may include weight loss, lethargy, anaemia, progressive paresis, enlarged
lymph nodes, petechiae of serous membranes, dependent oedema, urticarial and
exudation of the coronary bands.
Introduction to Trypanosoma evansi
2
MIN
MAX
The PCR Max qPCR Kit for Trypanosoma evansi (T.evansi) genomes is designed for the
in vitro quantification of T.evansi genomes. The kit is designed to have the broadest
detection profile possible whilst remaining specific to the T.evansi genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
T.evansi sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
3
Kit Contents
• T.evansi specific primer/probe mix (150 reactions BROWN)
FAM labelled
• T.evansi positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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