PCRmax Trichophyton mentagrophytes Instruction Manual

Heat shock protein 60 kDa
(hsp60) gene

Trichophyton
mentagrophytes

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Trichophyton mentagrophytes is a keratinophylic fungus belonging to a homogeneous
group of fungi called the dermatophytes. The dermatophytes cause a variety of cutaneous
infections in humans and animals. They belong to the genus Trichophyton and are
characterized by the development of both smooth-walled macro- and microconidia.
Macroconidia are mostly borne laterally directly on the hyphae or on short pedicels, and
are thin- or thick-walled, clavate to fusiform, and range from 4 to 8 by 8 to 50 μm in size.
Macroconidia are few or absent in many species. Microconidia are spherical, pyriform to
clavate or of irregular shape, and range from 2 to 3 by 2 to 4 μm in size.

T. mentagrophytes has at least five different variants which make up the Trichophyton
mentagrophytes complex. The organism has variable characteristics with anthropophilic
form producing sparse aerial mycelium with numerous spores. The zoophilic isolate
produces powdery or granular colonies. Microscopically the most consistent feature of T.
mentagrophytes is the production of globose microaleuriospores arranged in grape-like
clusters. T. mentagrophytes assimilates phosphorus, potassium, sodium and calcium. It
utilizes methionine but is inhibited by folic acid. The organism has been recovered from a
variety of sources such as soil, floor of swimming pools, hairs of wild boar, cats and dogs,
farm animals, foot wears, shower stalls and from human toe webs without clinical lesions.

T. mentagrophytes breaks down keratinous substrates by both chemical and mechanical
ways. Five different keratinolytic enzymes from ten strains of T. mentagrophytes have
been isolated. These enzymes are known to play a role in pathogenesis of infections
caused by these organisms in both humans and animals.

Introduction to Trichophyton mentagrophytes

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MIN

MAX

While the kit has been designed for Trichophyton mentagrophytes, due to the high level
of homology between fungi species, the assay will also detect Trichophyton verrucosum,
Arthroderma benhamiae and Trichophyton rubrum.

The PCR Max qPCR Kit for Trichophyton mentagrophytes (T.mentagrophytes) genomes is
designed for the in vitro quantification of T.mentagrophytes genomes. The kit is designed
to have the broadest detection profile possible whilst remaining specific to the T.
mentagrophytes genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
T.mentagrophytes sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• T.mentagrophytes specific primer/probe mix (150 reactions BROWN)

FAM labelled

• T.mentagrophytes positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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