PCRmax Trichomonas vaginalis Instruction Manual

Putative beta-tubulin gene

Trichomonas vaginalis

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Trichomonas vaginalis is a protozoan parasite of the Trichomonas genus. The single­celled organism has an oval shaped body with 5 flagella; four of these extend together in a
bundle out of the cell, anteriorly near the cytosome, with the fifth wrapping backwards
along the cell surface. There is also an axostyle found at the base of the flagella. This
anaerobic organism infects humans causing trichomoniasis, a disease of the female
reproductive tract.

Trichomoniasis is a sexually transmitted disease that infects the male urethra and the
female vagina or urethra. Infection of these cells is thought to be facilitated by the axostyle.
Infection may cause tissue damage resulting in inflammation. However, the infection can
be cleared with the use of antibiotics.

In men, this infection can cause a discharge from the penis or pain when passing urine. In
women, the infection can present with soreness, inflammation and itching of the vaginal
area and may include vaginal discharge or pain when passing urine. However, many cases
may be asymptomatic. In pregnant women, the presence of this organism can lead to
premature birth, low birth weight, and increased mortality. There is also a link between T.
Vaginalis infection and HIV, with infection with this organism thought to be a predisposing
factor.

Introduction to Trichomonas vaginalis

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MIN

MAX

The primers have 100% homology with the principal reference sequences with the NCBI
database for beta-tubulin (L05468.1, L05470.1, XM_001284521.1, XM_001301717.1,
XM_001311949.1, XM_001321203.1, XM_001579619.1, XM_001582993.1).

The PCR Max qPCR Kit for Trichomonas vaginalis (T.vaginalis) genomes is designed for
the in vitro quantification of T.vaginalis genomes. The kit is designed to have the broadest
detection profile possible whilst remaining specific to the T.vaginalis genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
T.vaginalis sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• T.vaginalis specific primer/probe mix (150 reactions BROWN)

FAM labelled

• T.vaginalis positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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