PCRmax Toxoplasma gondii Instruction Manual

Repeat region

Toxoplasma gondii

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Toxoplasma gondii is a species of parasitic protozoa in the genus Toxoplasma.[1] The
definitive host of T. gondii is the cat, but the parasite can be carried by the vast majority of
warm-blooded animals, including humans. The causative agent of toxoplasmosis, the
disease, is usually minor and self-limiting but can have serious or even fatal effects on a
fetus whose mother first contracts the disease during pregnancy or on an
immunocompromised human or cat.

Acute stage toxoplasma infections can be asymptomatic, but often gives flu-like symptoms
in the early acute stages, and like flu can become, in very rare cases, fatal. The acute
stage fades in a few days to months, leading to the latent stage. Latent infection is
normally asymptomatic; however, in the case of immunocompromised patients (such as
those infected with HIV or transplant recipients on immunosuppressive therapy),
toxoplasmosis can develop. The most notable manifestation of toxoplasmosis in
immunocompromised patients is toxoplasmic encephalitis, which can be deadly. If infection
with T. gondii occurs for the first time during pregnancy, the parasite can cross the
placenta, possibly leading to hydrocephalus, intracranial calcification, and chorioretinitis,
with the possibility of spontaneous abortion or intrauterine death.

Introduction to Toxoplasma gondii

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MIN

MAX

The primers have 100% homology with all reference sequences included in the NCBI
database (EF195646.1, AF487550.1, AF146527).

The PCR Max qPCR Kit for Toxoplasma gondii (T.gondii) genomes is designed for the in
vitro quantification of T.gondii genomes. The kit is designed to have the broadest
detection profile possible whilst remaining specific to the T.gondii genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
T.gondii sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• T.gondii specific primer/probe mix (150 reactions BROWN)

FAM labelled

• T.gondii positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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