PCRmax Theileria parva Instruction Manual
microneme-rhoptry antigen
(p104) gene
Theileria parva
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
1
Theileria parva is a parasitic protozoan with a small genome of 10-12 Mbp. This species
has four chromosomes and a plastome which contains the genes for the apicoplast
organelles. T. parva infects lymphocytes causing Theileriosis or East Coast fever in cattle,
African buffalo (Syncerus caffer), water buffalo (Bubalus bubalis), and waterbucks.
T.parva uses Ioxdidae ticks usually Rhipicephalus appendiculatus as a vector. Protozoal
sporozoites are formed in the salivary glands of infected ticks and are subsequently
transmitted to cattle during feeding. After transmission, the protozoan life cycle continues
resulting in Schizonts or Koch‘s blue body forming in the host lymph nodes. These
schizonts subsequently invade erythrocytes and become piroplasms. The host blood cells
are then ingested by uninfected larval ticks, once in the gut, T.parva undergoes sexual
replication and is then released to the salivary glands for infection of new hosts.
The symptoms of East Coast fever include anorexia, fever and enlargement of the lymph
nodes resulting in lymphadenopathy. Other symptoms including diarrhoea, nasal
discharge and corneal opacity are less common. Infection with this pathogen can also
cause a neurologic disorder called "turning sickness" which is the result of capillary
blockage by the infected erythrocytes.
Several drugs are available for the treatment of Theileriosis. It can also be prevented by
inoculating the animal with a high dose of sporozites harvested from ticks.
Introduction to Theileria parva
2
MIN
MAX
The PCR Max qPCR Kit for Theileria parva (T.parva ) genomes is designed for the in vitro
quantification of T.parva genomes. The kit is designed to have the broadest detection
profile possible whilst remaining specific to the T.parva genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
T.parva sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
3
Kit Contents
• T.parva specific primer/probe mix (150 reactions BROWN)
FAM labelled
• T.parva positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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