PCRmax Tannerella forsythia Instruction Manual

Heat shock protein 60 gene
(hsp60 gene)

Tannerella forsythia

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Tannerella Forsythia, formerly known as Bacteroides forsythus, is a fusiform, fastidious,
anaerobic, Gram-negative organism that resides in the deep periodontal pockets of
humans. It belongs to the genus Bacteroides and forms a part of the oral microbiota. It is
covered by a crystalline surface (S-layer) which is a 6-nm thick, closed, monolayer that is
formed by co-assembly of the two S-layer glycoproteins. This organism is synergistic with
Porphyromonas gingivalis, and the presence of both accelerates progression of the
disease. Pathogenic factors include a trypsin-like protease (PrtH) and a variety of
glycosidases. Other virulence factors include the cell surface adhesin, BspA, that mediates
adherence to fibronectin and other extracellular matrix components. The genome is
approximately 3405 kb long and contains 3034 predicted open reading frames.

Proteolytic enzymes in Tannerella Forsythia are responsible for degradation of host
proteins, providing essential amino acids, peptides and haem for growth. They contribute
to the bacterial virulence in a number of ways such as by degrading host periodontal
tissues, activating host degradative enzymes, modifying host cell proteins to expose
cryptotopes for bacterial colonization, cleaving components involved in innate and adaptive
immunity and activating components involved in clotting/fibrinolyis. They have the ability to
induce apoptosis and invade the periodontal pocket along with P.gingivalis, attacking the
host’s white blood cells. The induction of host cell apoptosis results in the elimination of
host immune cells which further supports the bacterial colonization of the periodontal
pockets. T. forsythia is generally sensitive to antibiotics that are active against anaerobes.

Periodontitis is the disruption of connective tissue attachment along with the adjacent
alveolar bone. Some symptoms include development of new spaces between the teeth,
change in the manner that the teeth fit together when biting and swelling of the gums.
Periodontitis is significantly correlated with an overgrowth of a large number of anaerobic
bacteria so this disease can be suppressed by good oral hygiene and by utilizing
antimicrobial agents that target the participating anaerobes.

Introduction to Tannerella forsythia

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MIN

MAX

The PCR Max qPCR Kit for Tannerella forsythia (T.forsythia) genomes is designed for the
in vitro quantification of T.forsythia genomes. The kit is designed to have the broadest
detection profile possible whilst remaining specific to the T.forsythia genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
T.forsythia sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• T.forsythia specific primer/probe mix (150 reactions BROWN)

FAM labelled

• T.forsythia positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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