PCRmax Streptococcus salivarius Instruction Manual

Methionine aminopeptidase
(map) gene

Streptococcus
salivarius

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Streptococcus salivarius is a species of Gram-positive cocci bacteria. S. salivarius is a
normal inhabitant of the upper respiratory tract. It may enter the blood stream by accident
during dental work or when brushing the teeth, where it has been implicated in septicemia
cases in neutropenic patients, a condition that shows abnormally low levels of neutrophils
in the blood. It is the first bacterium which colonizes the dental plaque, before being joined
by numerous other species of various genera. The genome is between 2.1 and 2.2 Mbp
with 10% of the genes are not functional due to frameshifts, nonsense mutations, deletions
or pseudogenes. S. salivarius is approximately 2 µm in size and are spherical in shape.
The cocci usually occur in pairs and short chains and are facultative anaerobes. S.
salivarius contains fimbriae on their cell surface and are involved in co-aggregation of S.
salivarius with the periodontopathogen Prevotella intermeida .

Viridans streptococci species cause most dental caries and are the most frequent cause of
subacute native valve bacterial endocarditis, typically associated with dental procedures .
S. salivarius has also been implicated in meningitis cases. Most cases of meningitis are
usually due to a complication of neurosurgical or anesthesia procedures, but can occur as
a non-iatrogenic infection.

Symptoms of S.salivarius meningitis typically begin to appear in one day with typical
meningitis sypmptoms presented. Endocaritis cases show an inflammation of the inner
layer of the heart. Streptococcus salivarius secretes a glucosltransferase (Gtf) which
forms a glucan from sucrose. S. salivarius is one of the main sources of Gtf in saliva and in
the acquired pellicle is believed to be from S. salivarius resident on the dorsum of the
tongue. Gtfs incorporated in the pellicle are known to be active and to form glucans to
which other oral streptococci, such as the mutans streptococci, are able to adhere. Some
strains of S. salivarius are being trialed for their use as a probiotic in the prevention of oral
infections. Some strains of S. salivarius are found to produce BLIS (Bacteriocin-like
Inhibitory Substances) which is an antimicrobial peptide. This peptide displays interspecies
inhibition, and inhibits Streptococcus pyogenes (which causes Strep throat infections).

Introduction to Streptococcus salivarius

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MIN

MAX

The PCR Max qPCR Kit for Streptococcus salivarius (S.salivarius) genomes is designed
for the in vitro quantification of S.salivarius genomes. The kit is designed to have the
broadest detection profile possible whilst remaining specific to the S.salivarius genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
S.salivarius sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• S.salivarius specific primer/probe mix (150 reactions BROWN)

FAM labelled

• S.salivarius positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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