PCRmax Streptococcus oralis Instruction Manual

glucosyltransferase (gtfR) gene

Streptococcus oralis

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Streptococcus oralis is a Gram-positive, non motile, α-haemolytic bacterium of the viridans
group of Streptococcus genus. The double-stranded DNA genome is circular containing
just over 2Mbp. As a part of the healthy oral flora, S. oralis is often found in plaque and
dental cavities but is also capable of causing bacterial endocarditis and septicaemia in
susceptible individuals.
S. oralis enters the bloodstream via open wounds such as those created during oral
surgery and consequently causes platelet aggregation and oxidation of iron in
haemoglobin. Protease activity targeted at host IgA weakness the immune defense.
Bacterial endocarditis presents with loss of appetite, rash, joint pain, fatigue and prolonged
fever lasting two to three days in a person with congenital heart disease. Treatment is
targeted at eradication of the bacterium from the infection site and bactericidal agents such
as Penicillin G in aqueous crystalline form are given parenterally to achieve this.
Antibiotics are commonly given to high risk individuals before procedures with a risk of
bacterial entry into bloodstream.

Introduction to Streptococcus oralis

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MIN

MAX

Our kit for S. oralis has been designed for the specific and exclusive in vitro
quantification of all S. oralis isolates and no other streptococcus species.
Glucosytranferase genes have previously been shown to be a good target sequences for
the differential detection of Streptococcus species in other clinical real time PCR based
studies (W. kim Seow et.al., 2009). The primers and probe sequences in this kit have
100% homology with a broad range of clinically relevant reference sequences based on a
comprehensive bioinformatics analysis.

The PCR Max qPCR Kit for Streptococcus oralis (S.oralis) genomes is designed for the in
vitro quantification of S.oralis genomes. The kit is designed to have the broadest detection
profile possible whilst remaining specific to the S.oralis genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
S.oralis sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• S.oralis specific primer/probe mix (150 reactions BROWN)

FAM labelled

• S.oralis positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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