PCRmax Streptococcus mutans Instruction Manual

glucosyltransferase-I (gtfb) gene

Streptococcus mutans

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Streptococcus mutans is a Gram-positive faculatively anaerobic bacterium with a genome
of over 2Mbp. The genome is arranged in a circular conformation with 3 separate plasmids
of approximately 5Kbp each. This bacterium thrives between 18 and 40°C and can be
found inhabiting the oral cavity of almost all humans.

S. mutans in the oral cavity bind to the enamel of teeth and replicate to form a biofilm. The
layer of bacteria metabolise carbohydrates into glucose and fructose with the former
allowing the bacteria to attach to the enamel surface via adhesive glucans and the latter
providing energy for bacterial replication. The fermentation of these sugars produces an
acidic environment in the mouth that decalcifies the tooth enamel resulting in cavities and
potentially tooth loss. The formation of the biofilm makes this bacterium difficult to treat
with antibiotics as their antibiotic tolerance can increase dramatically.

Introduction to Streptococcus mutans

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MIN

MAX

The glucosyltransferase-I gene has previously been identified as a highly specific market
for S. mutans (Lett Appl Microbiol. 2006 Feb; 42(2):127-31). The primers and probe have
100% homology with all reference sequences for S. mutans in the NCBI database.

AE014133.1, M17361.1, D88654.1, D88651.1, D89977.1, D88660.1, D88657.1

The PCR Max qPCR Kit for Streptococcus mutans (S.mutans) genomes is designed for
the in vitro quantification of S.mutans genomes. The kit is designed to have the broadest
detection profile possible whilst remaining specific to the S.mutans genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
S.mutans sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• S.mutans specific primer/probe mix (150 reactions BROWN)

FAM labelled

• S.mutans positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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