PCRmax Streptococcus agalactiae Instruction Manual
cAMP factor (cfb) gene
Streptococcus
agalactiae
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
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Streptococcus is a genus of non-motile, Gram-negative bacteria which are
characteristically round or oval in shape and are usually found in pairs or chains. Species
within this genus have genomes of around 2Mbp in length.
Streptococci infections are divided into one of several groups, of which groups A and B are
the
most significant, causing the most devastating human diseases.
Group B infections cause illness in people with weakened immune systems including
pregnant women, new born babies and the elderly. In most cases, the bacteria can be
found in the gastrointestinal, urinary or genital tract but do not cause symptoms unless
they infect the blood stream.
In new-born babies, early-onset Group B streptococcus (GBS) infections result in poor
feeding, lethargy and low blood pressure which can be fatal in around 12% of cases. Late
onset GBS is classified as when symptoms are shown more than 2 days after birth and
account for only 10% of the GBS cases in new born babies. Late onset infections usually
result in meningitis with septicemia.
Introduction to Streptococcus agalactiae
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MIN
MAX
The primer and probe share 100% homology with all sequences in the NCBI database at
the time of product launch.
The PCR Max qPCR Kit for Streptococcus agalactiae (S.agalactiae) genomes is designed
for the in vitro quantification of S.agalactiae genomes. The kit is designed to have the
broadest detection profile possible whilst remaining specific to the S.agalactiae genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
S.agalactiae sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
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Kit Contents
• S.agalactiae specific primer/probe mix (150 reactions BROWN)
FAM labelled
• S.agalactiae positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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