PCRmax Staphylococcus epidermidis Instruction Manual
superoxide dismutase (sodA)
gene
Staphylococcus
epidermidis
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
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Staphylococcus epidermidis is a Gram-positive, non-motile bacterium of the
Staphylococcus genus. This bacterium has a genome of 2.5Mbp in length arranged in
circular formation. S. epidermidis is part of the natural human flora and can be found on
mucosal membranes and skin but is one of the most common causes of noscomial
infections.
S. epidermidis has the ability to form a multilayer biofilms on inert objects, such as
intravenous catheters and other plastic medical devices. In this conformation, antibiotic
dispersion is much reduced. This, coupled with high antibiotic resistance due to regular
exposure results in reduced effect of antibiotic treatment.
Introduction to Staphylococcus epidermidis
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MIN
MAX
The target sequence (sodA) has previously been shown to be a good genetic marker for
S. epidermidis in other real time PCR based studies (Tadayuki Iwase et.al 2007). The
primers and probe sequences in this kit have 100% homology with a broad range of
clinically relevant reference sequences based on a comprehensive bioinformatics
analysis.
The PCR Max qPCR Kit for Staphylococcus epidermidis (S.epidermidis) genomes is
designed for the in vitro quantification of S.epidermidis genomes. The kit is designed to
have the broadest detection profile possible whilst remaining specific to the S.epidermidis
genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
S.epidermidis sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
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Kit Contents
• S.epidermidis specific primer/probe mix (150 reactions BROWN)
FAM labelled
• S.epidermidis positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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