PCRmax Staphylococcus aureus Instruction Manual
FEMB gene (chromosomal gene)
Staphylococcus aureus
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
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Staphylococcus aureus is a spherical, Gram-positive bacterium of the Staphylococcaceae
family. The genome of this species is around 2.8Mb in length. Virulence factors for S.
aureus are encoded by phages, pathogenicity islands, staphylococcus cassette
chromosome and plasmids, which contain genes for antibiotic resistance such as those
found in Methicillin-Resistant Staphylococcus aureus (MRSA).
S. aureus can be found on the skin and mucosal membranes of healthy individuals, but
any abrasion to these barriers allows the bacterium to enter the wound, colonise and
cause infection. As this bacterium lives on the skin, it can be transmitted easily between
individuals and is commonly seen in hospital acquired infections.
S. aureus can cause a variety of different infections ranging from mild skin infections such
as impetigo to invasive diseases and toxic mediated diseases. The production of
extotoxins known as Pyrogenic toxin superantigens in some strains of S. aureus can cause
toxic shock syndrome.
Introduction to Staphylococcus aureus
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MIN
MAX
FEMB is a chromosomal gene specific to S. aureus. The primers and probe have 100%
homology with all reference sequences in the NCBI database including those listed
below.
FEMB sequence specificity
CP000703.1, BA000017.4, CP000046.1, CP000253.1, CP000255.1, X17688.1,
X571857.1, BA000033.2, BA000018.3, BX571856.1
The PCR Max qPCR Kit for Staphylococcus aureus (S.aureus) genomes is designed for
the in vitro quantification of S.aureus genomes. The kit is designed to have the broadest
detection profile possible whilst remaining specific to the S.aureus genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
S.aureus sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
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Kit Contents
• S.aureus specific primer/probe mix (150 reactions BROWN)
FAM labelled
• S.aureus positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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