PCRmax Shewanella putrefaciens Instruction Manual

ferF pseudogene

Shewanella
putrefaciens

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Shewanella putrefaciens is a Gram-negative, rod-shape bacterium of the Shewanellaceae
family with a circular DNA genome of around 4.6Mbp in length. It is not usually a pathogen
of humans; however cases of benign skin and soft tissue infections, peritonitis, biliary tract
infection and bacteraemia in immuno-compromised patients have been reported. This
bacterium has also been found in fish, resulting in spoilage possibly by production of
trimethylamine.

S. putrefaciens is an opportunistic pathogen and associated with other bacteria in a wide
range of clinical infections particularly in polymicrobial bacteraemia. Naturally found in
water, aquatic environments are often implicated as the source of infection. The target of
this opportunistic infection is the skin with transfer to the bloodstream by as yet
uncharacterised mechanisms.

Predisposing factors to S. putrefaciens infection include prematurity, congenital pneumonia
and ulceration of the lower limbs. Infection in soft tissue can result in cellulitis, ulceration
and abscess formation. These infections are generally resistant to treatment with beta­lactam based antibiotics but sensitive to aminoglycosides.

Introduction to Shewanella putrefaciens

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MIN

MAX

The PCR Max qPCR Kit for Shewanella putrefaciens (S.putrefaciens) genomes is
designed for the in vitro quantification of S.putrefaciens genomes. The kit is designed to
have the broadest detection profile possible whilst remaining specific to the S.putrefaciens
genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
S.putrefaciens sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• S.putrefaciens specific primer/probe mix (150 reactions BROWN)

FAM labelled

• S.putrefaciens positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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