PCRmax Porphyromonas gingivalis Instruction Manual

Fimbrillin fimA(I) gene

Porphyromonas
gingivalis

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Porphyromonas gingivalis is an anaerobic, Gram-negative bacterium which is rod shaped
and non-motile. The circular genome has not been fully sequenced but is known to be over
2Mbp in length. Infection with this bacterium can lead to gingivitis and periodontitis.

P. gingivalis resides in the mouth within the gingival and is known to cause inflammation of
this soft tissue. This bacterium uses the fimbriae to attach to the gum layer. Once adhered
to the gingiva, the bacterium interacts with other bacteria that can be found here and in
doing so, form a biofilm known as plaque. If the plaque is left untreated, it can lead to the
formation of lesions between the tooth and the gingival layer. P. gingivalis is able to
express proteolytic enzymes that attack the ligaments that hold the tooth and gum
together. The continuation of this process eventually concludes in loosening of the tooth
from the gum and bone potentially resulting in loss of the tooth.

Infection with P. gingivalis leads to symptoms which include inflammation of the gums that
cause redness and may be painful, and bad breath.

Introduction to Porphyromonas gingivalis

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MIN

MAX

The fimbrillin gene has previously been identified as a highly specific market for P.
gingivalis (J. Periodont Res 2008; 43: 352-359). The primers and probe have 100%
homology with all reference sequences for P. gingivalis in the NCBI database.

AP009380.1, AB261608.1, AB261607.1, AB195793.1, AB195790.1, AB195789.1,
AB195788.1, AB195787.1, AB195786.1, 17799.1, D17798.1, D17797.1, D17796.1,
D17795.1, D17794.1, B004560.1, AB058848.1,

The PCR Max qPCR Kit for Porphyromonas gingivalis (P.gingivalis) genomes is designed
for the in vitro quantification of P.gingivalis genomes. The kit is designed to have the
broadest detection profile possible whilst remaining specific to the P.gingivalis genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
P.gingivalis sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• P.gingivalis specific primer/probe mix (150 reactions BROWN)

FAM labelled

• P.gingivalis positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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