PCRmax Plasmodium ovale Instruction Manual

plasmepsin gene
Plasmodium ovale
PCRmax Ltd qPCR test
TM
For general laboratory and research use only
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Plasmodium is a genus of protozoa that cause Malaria. Plasmodium species use two hosts in their lifecycle. Plasmodium ovale, which infects humans, uses Anophele mosquitoes as a vector. This species if found in West Africa, the Philippines and Indonesia.
As the mosquito bites a human, the sporozoites of the parasite are injected into the individual with the mosquito saliva. The sporozoites target the host liver where they replicate asexually creating merozoites which are subsequently released into the host circulatory system where they invade erythrocytes and undergo nuclear division causing these cells to burst. The parasite can also reproduce sexually producing gametocytes which are then able to infect the vector host when they bite the human. In the mid gut of the mosquito these mature into micro-gametes with flagella and macro-gametes that fuse together to produce a zygote which later develops into an oocyst in the space between the epithelial cells and the basal lamina. These oocysts undergo asexual replication to produce sporozoites of around 1µm in diameter which relocate to the salivary gland ready to infect another individual when the mosquito feeds again.
After an incubation period varying between 7 and 30 days, infection with P. ovale presents initially with fever, muscle pain, nausea. Symptoms including anaemia and confusion can also occur. Treatment is based on identification of the specific species causing the infection, where in the world the infection was acquired and drug resistance. P. ovale infections are most commonly treated with Primaquine which prevents the parasite being released from the liver cells into the bloodstream of the host.
Introduction to Plasmodium ovale
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MIN
MAX
The target sequence within the plasmepsin gene has very low homology with other species of plasmodia that cause malaria. The primers and probe sequences in this kit have 100% homology with a broad range of clinically relevant reference sequences based on a comprehensive bioinformatics analysis.
The PCR Max qPCR Kit for Plasmodium ovale (P.ovale) genomes is designed for the in vitro quantification of P.ovale genomes. The kit is designed to have the broadest detection profile possible whilst remaining specific to the P.ovale genome.
The primers and probe sequences in this kit have 100% homology with a broad range of P.ovale sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will answer your question.
Specificity
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Kit Contents
• P.ovale specific primer/probe mix (150 reactions BROWN)
FAM labelled
• P.ovale positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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