PCRmax Ornithobacterium rhinotracheale Instruction Manual

16S ribosomal RNA (16s) gene

Ornithobacterium
rhinotracheale

PCRmax Ltd qPCR test

TM

150 tests

For general laboratory and research use only

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Ornithobacterium rhinotracheale is a gram-negative rod-shaped bacterium previously
known as Pasteurella-like, Kingella-like or Pleomorphic Gram-Negative Rod (PGNR) and
TAXON 28. It is capable of causing respiratory disease in turkeys, chickens and other
avian species. Ornithobacterium cells are gram-negative, nonmotile, nonsporulating, short,
plump rods with chemoorganotrophic, mesophilic metabolism. When cultured on solid
media, the bacterium appears as short, and plump rods measuring 0.2-0.9 µm in width and

0.6-5 µm in length ,and less frequently as long filamentous rods or club-shaped rods. It has
a genome approximately 2.36MB in size.

The presence of O.rhinotracheale in commercial poultry and also in wild birds has been
shown to be worldwide,meaning that there is a broad potential reservoir. O.rhinotracheale
has been shown to be transmitted via aerosol transmission. It has also a secondary
infarction that affects birds that have been infected by viruses such as Turkey
rhinotracheitis virus or Newcastle Disease virus. Factors such as stress, inadequate
ventilation, poor hygiene, high ammonia levels have also been linked to the disease
manifesting itself in birds that are carriers. Transmission of O.rhinotracheale is possible not
only horizontally through aerosols but also vertically through the egg.

The clinical symptoms associated with the isolation of O. rhinotracheale included
weakness, gasping, severe dyspnoea, mucus discharge and mortality. Dissection showed
fibrinopurulent pneumonia and fibrinous inflammation of the thoracic air sacs. O.
rhinotracheale also can cause sudden deaths in young birds through infections of the
brains and the skull, featuring totally weakened skull-bones. In turkeys of 12 weeks of age
or older, O.rhinotracheale can cause acute pneumonia with mortality rates of up to 50%. O.
rhinotracheale infection in older turkeys causes paralysis through arthritis, osteitis and
osteomyelitis, commonly showing a purulent, slimy exudate in the joints of the lame birds.
From the eighteen serotypes, serotype A is predominant among the chicken-isolates (96%)
and the most frequent (54%) among the turkey isolates, which are more heterogeneously
divided. Up to now there is no explanation for these differences in distribution but it has
been shown that serotype A and C strains from chickens and serotype B, D and E strains
from turkeys have a similar virulence for both chickens and turkeys.

Introduction to Ornithobacterium rhinotracheale

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MIN

MAX

Our kit for Ornithobacterium rhinotracheale has been designed for the specific and
exclusive in vitro quantification of the Ornithobacterium rhinotracheale species including
all sub species. The 16S ribosomal gene, is the ideal target to achieve a broad based
detection profile for all subspecies within this species. The primers and probe sequences
in this kit have 100% homology with a broad range of clinically relevant reference
sequences based on a comprehensive bioinformatics analysis.

The PCR Max qPCR Kit for Ornithobacterium rhinotracheale (O.rhinotracheale) genomes
is designed for the in vitro quantification of O.rhinotracheale genomes. The kit is designed
to have the broadest detection profile possible whilst remaining specific to the O.
rhinotracheale genome.

The primers and probe sequences in this kit have 100% homology with a broad range of
O.rhinotracheale sequences based on a comprehensive bioinformatics analysis.

If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to [e-mail] and our bioinformatics team will
answer your question.

Specificity

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Kit Contents

• O.rhinotracheale specific primer/probe mix (150 reactions BROWN)

FAM labelled

• O.rhinotracheale positive control template (for Standard curve RED)

• Internal extraction control primer/probe mix (150 reactions BROWN)

VIC labelled as standard

• Internal extraction control DNA (150 reactions BLUE)

• Endogenous control primer/probe mix (150 reactions BROWN)

FAM labelled

• RNAse/DNAse free water (WHITE)

for resuspension of primer/probe mixes and internal extraction control DNA

• Template preparation buffer (YELLOW)

for resuspension of positive control template and standard curve preparation

Reagents and equipment to be supplied by the user

Real-Time PCR Instrument

DNA extraction kit

This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.

Lyophilised 2x qPCR Mastermix

This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.

Pipettors and Tips

Vortex and centrifuge

Thin walled 1.5 ml PCR reaction tubes

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