PCRmax Naegleria fowleri Instruction Manual
Naegleria fowleri
PCRmax Ltd qPCR test
TM
150 tests
For general laboratory and research use only
1
Naegleria fowleri also known as the “brain eating amoeba” is a bacteria-eating amoeba
that can potentially be pathogenic. It can cause naegleriasis, a sudden and severe brain
infection.
Naegleria fowleri is commonly found in warm freshwater and soil and is the only species
within the genus of Naegleria that is known to infect humans.
Infection does not occur through drinking contaminated water but only if the water goes up
into the nasal cavity. The amoeba travels upwards through the cavity to the brain where it
destroys the brain tissue.
Naegleria fowleri is a thermophilic organism, growing optimally at temperatures up to
115ºF (46.1ºC) and so infections are more prevalent in the summer months.
Symptoms of Naegleria fowleri infections maybe be similar to those of bacterial meningitis
in its early stages. It cannot be transmitted from one person to another and the symptoms
begin to show between 1-9 days after infection. After the onset of the symptoms the
disease progresses extremely rapidly and usually causes death within 1-12 days through
brain swelling. As a result, fast and accurate detection is critical. Only 3 people out of 138
individuals infected with Naegleria fowleri in the United states from 1962-2015 have been
known to survive, putting the fatality rate at over 97%.
Introduction to Naegleria fowleri
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MIN
MAX
The PCR Max qPCR Kit for Naegleria fowleri (N.fowleri) genomes is designed for the in
vitro quantification of N.fowleri genomes. The kit is designed to have the broadest
detection profile possible whilst remaining specific to the N.fowleri genome.
The primers and probe sequences in this kit have 100% homology with a broad range of
N.fowleri sequences based on a comprehensive bioinformatics analysis.
If you require further information, or have a specific question about the detection profile of
this kit then please send an e.mail to help@pcrmax.com and our bioinformatics team will
answer your question.
Specificity
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Kit Contents
• N.fowleri specific primer/probe mix (150 reactions BROWN)
FAM labelled
• N.fowleri positive control template (for Standard curve RED)
• Internal extraction control primer/probe mix (150 reactions BROWN)
VIC labelled as standard
• Internal extraction control DNA (150 reactions BLUE)
• Endogenous control primer/probe mix (150 reactions BROWN)
FAM labelled
• RNAse/DNAse free water (WHITE)
for resuspension of primer/probe mixes and internal extraction control DNA
• Template preparation buffer (YELLOW)
for resuspension of positive control template and standard curve preparation
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
DNA extraction kit
This kit designed to work well with all processes that yield high quality DNA with minimal
PCR inhibitors.
Lyophilised 2x qPCR Mastermix
This kit is designed to be compatible with all commercially available Mastermixes that run
with standard cycling conditions.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes
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